Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 728,706 G→A 8.6% I9I (ATC→ATT)  kdpA ← K(+) transporting P‑type ATPase subunit KdpA

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913728,7060GA 8.6% 113.1 / 3.2 48I9I (ATC→ATT) kdpAK(+) transporting P‑type ATPase subunit KdpA
Reads supporting (aligned to +/- strand):  ref base G (24/19);  new base A (2/2);  total (26/22)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.48e-01

TCTCACGGTCAGAGACGCCAAGTGCGCGAAAAAGTACGCGCTCAACGCCCGTTGTACCGGGAAGAGGAATGTCATTAATCAGCCGCGCCAGCCCGCTGCCTAAAGGACGCGCCAGCACCATTAACACCAGTAAAAACGTGGCGATCAGTAAGAACCCTTGCGCAGCCATCAGAACGCCTCCGCATTGATCAGGGCATAAACCAGATAACCCAGTAATAAAAACACCAGCAATACGCCGGTTATCACGCCTGCACTCACAGTGCACCTCCAGTGGCCTAAAAG  >  NC_000913/728564‑728845
                                                                                                                                              |                                                                                                                                           
tctcACGGTCAGAGACGCCAAGTGCGCGAAAAAGTACGCGCTCAACGCCCGTTGTACCGGGAAGAGGAATGTCATTAATCAGCCGCGCCAGCCCGCTGCCTAAAGGACGCGCCAGCACCATTAACACCAGTAAAAACGTGGCg                                                                                                                                             >  1:778021/1‑143 (MQ=255)
      ggTCAGAGACGCCAAGTGCGCGAAAAAGTACGCGCTCAACGCCCGTTGTACCGGGAAGAGGAATGTCATTAATCAGCCGCGCCAGCCCGCTGCCTAAAGGACGCGCCAGCACCATTAACACCAGTAAAAACGTGGCGATCAGt                                                                                                                                       >  1:873818/1‑143 (MQ=255)
       gTCAGAGACGCCAAGTGCGCGAAAAAGTACGCGCTCAACGCCCGTTGTACCGGGAAGAGGAATGTCATTAATCAGCCGCGCCAGCCCGCTGCCTACTTGTCGCGTCAGCACCATTAACACCAGTAAAAACGTGGCGATCAGTa                                                                                                                                      <  1:696402/143‑1 (MQ=255)
                 ccAAGTGCGCGAAAAAGTACGCGCTCAACGCCCGTTGTACCGGGAAGAGGAATGTCATTAATCAGCCGCGCCAGCCCGCTGCCTAAAGGACGCGCCAGCACCATTAACACCAGTAAAAACGTGGCGATCAGTAAGAACCCTTg                                                                                                                            <  1:890617/143‑1 (MQ=255)
                   aaGTGCGCGAAAAAGTACGCGCTCAACGCCCGTTGTACCGGGAAGAGGAATGTCATTAATCAGCCGCGCCAGCCCGCTGCCTAAAGGACGCGCCAGCACCATTAACACCAGTAAAACACTGGAGACCAGTAAGACCCCTCgcc                                                                                                                          >  2:733148/1‑142 (MQ=255)
                   aaGTGCGCGAAAAAGTACGCGCTCAACGCCCGTTGTACCGGGAAGAGGAATGTCATTAATCAGCCGCGCCAGCCCGCTGCCTAAAGGACGCGCCAGCACCATTAACACCAGTAAAAACGTGGCGATCAGTAAGAACCCTTgcg                                                                                                                          >  1:447343/1‑143 (MQ=255)
                           gAAAAAGTACGCGCTCAACGCCCGTTGTACCGGGAAGAGGAATGTCATTAATCAGCCGCGCCAGCCCGCTGCCTAAGGGACGCGCCAGCACCATTAACACCACTAAAAACGTGGCGACCAGTAAGAACCCTTGCGCAGCCAta                                                                                                                  >  2:60935/1‑142 (MQ=255)
                           gAAAAAGTACGCGCTCAACGCCCGTTGTACCGGGAAGAGGAATGTCATTAATCAGCCGCGCCAGCCCGCTGCCTAAAGGACGCGCCAGCACCATTAACACCAGTAAAAACGTGGCGATCAGTAAGAACCCTTGCGCAGCCATc                                                                                                                  >  1:837675/1‑143 (MQ=255)
                           gAAAAAGTACGCGCTCAACGCCCGTTGTACCGGGAAGAGGAATGTCATTAATCAGCCGCGCCAGCCCGCTGCCTAAAGGACGCGCCAGCACCATTAACACCAGTAAAAACGTGGCGAACAGTAAGAACCCTTGCGCAGCCATc                                                                                                                  >  2:88431/1‑143 (MQ=255)
                              aaaGTACGCGCTCAACGCCCGTTGTACCGGGAAGAGGAATGTCATTAATCAGCCGCGCCAGCCCGCTGCCTAAAGGACGCGCCAGCACCATTAACACCAGTAAAAACGTGGCGCTCCGCACGAACCCTTGCGCAGCCATCAGa                                                                                                               >  2:898850/1‑143 (MQ=255)
                                  tACGCGCTCAACGCCCGTTGTACCGGGAAGAGGAATGTCATTAATCAGCCGCGCCAGCCCGCTGCCTAAAGGACGCGCCAGCACCATTAACACCAGTAAAAACGTGGCGATCAGTAAGAACCCTTGCGCAGCCATCAGAACGc                                                                                                           <  1:728838/143‑1 (MQ=255)
                                   aCGCGCTCAACGCCCGTTGTACCGGGAAGAGGAATTAAATTGATCATCCGCGCCCGCCCGCTGCCTAAATTACACGTTACTTCCATTAATACCAGTAAAAACGTGTCGATCAGTAACAACCCTTGCGCAGCCATCAGAACTcc                                                                                                          >  2:941980/1‑143 (MQ=255)
                                    cgcgCTCAACGCCCGTTGTACCGGGAAGAGGAATGTCATTAATCAGCCGCGCCCGCCCGCTGCCTCAAGGACGCGCCAGCACCATTAACACCAGTAAAAACGTGGCGACCCGTAAGAACCCTCGCGCAGCCACCAGAACGCCt                                                                                                         >  2:87161/1‑143 (MQ=255)
                                         tCAACGCCCGTTGTACCGGGAAGAGGAATGTCATTAATCAGCCGCGCCAGCCCGCTGCCTAAAGGACGCGCCAGCACCATTAACACCAGTAAAAACGTGGCGATCAGTAAGAACCCTTGCGCAGCCATCAGAACGCCTCCGCa                                                                                                    >  1:603893/1‑143 (MQ=255)
                                              gCCCGTTGTACCGGGAAGAGGAATGTCATTAATCAGCCGCGCCAGCCCGCTGCCTAAAGGACGCGCCAGCACCATTAACACCAGTAAAAACGTGGCGATCAGTAAGAACCCTTGCGCAGCCATCAGAACGCGTCCGCATTGAt                                                                                               >  1:161689/1‑143 (MQ=255)
                                               cccGTTGTACCGGGAAGAGGAATGTCATTAATCAGCCGCGCCAGCCCGCTGCCTAAAGGACGCGCCAGCACCATTAACACCAGTAAAAACGTGGCGATCAGTAAGAACCCTTGCGCAGCCATCAGAACGCCTCCGGATTGATc                                                                                              >  1:247838/1‑143 (MQ=255)
                                                  gTTGTACCGGGAAGAGGAATGTCATTAATCAGCCGCGCCAGCCCGCTGCCTAAAGGACGCGCCAGCACCATTAACACCAGTAAAAACGTGGCGATCAGTAAGAACCCTTGCGCAGCCATCAGAACGCCTCCGCATTGATCAgg                                                                                           <  2:293668/143‑1 (MQ=255)
                                                  gTTGGACCGGGAAGAGGAATGTCATTAATCAGCCGCGCCAGCCCGCTGCCTAAAGGACGCGCCAGCACCATTAACACCAGTAACAACGTGGCGATCAGTAAGAACCCTTGCGCAGCCATCAGAACGCCTCCGCATTGATCAgg                                                                                           <  1:94168/143‑1 (MQ=255)
                                                   ttGTACCGGGAAGAGGAATGTCATTAATCAGCGGTGAATCTCCTCTGCCGAAAGGACGCGCCAGCACCATTAACACCAGTAAAAACGTGGCGATCAGTAAGAACCCTTGCGCAGCCATCAGAACGCCTCCGCATTGATCAggg                                                                                          <  2:446106/143‑1 (MQ=255)
                                                    tggaCCCGGAAGAGGAACGTCATTAATCAGCCGCGCCAGCCCGCTGCCTAAAGGACGCGCCAGCACCATTAACACCAGTAAAAACGTGGCGCTCAGTAAGAACCCTTGCGCAGCCATCAGAACGCCTCCGCATTGATCAGGGc                                                                                         <  1:358788/140‑1 (MQ=255)
                                                        ccGGGAAGAGGAATGTCATTAATCAGCCGCGCCAGCCCGCTGCCTAAAGGACGCGCCAGCACCATTAACACCAGTAAAAACGTGGCGATCAGTAAGAACCCTTGCGCAGCCATCAGAACGCCTCCGCATTGATCAGGGCATaa                                                                                     >  1:711660/1‑143 (MQ=255)
                                                           ggAAGAGGAATGTCATTAATCAGCCGCGCCAGCCCGCTGCCTAAAGGACGCGCCAGCACCATTAACACCAGTAAAAACGTGGCGATCAGTAAGAACCCTTGCGCAGCCATCAGAACGCCTCCGCATTGATCAGGGCATAAAcc                                                                                  >  1:605832/1‑143 (MQ=255)
                                                             aaGAGGAATGTCATTAATCAGCCGCGCCAGCCCGCTGCCTAAAGGACGCGCCAGCACCATTAACACCAGTAAAAACGTGGCGATCAGTAAGAACCCTTGCGCAGCCATCAGAACGCCTCCGCATTGATCAGGGCATAAACCAg                                                                                >  2:314131/1‑143 (MQ=255)
                                                             aaGAGGAATGTCATGAATCAGCCGCGCCAGCCCGCTGCGTAAAGGACGCGCCAGCACCATTAACCCCAGTAAAAACGTGGCGATCAGTAAGAACCCCTGCGCAGCCATCAGAACGCCTCCGCCTTGATCAGGGCATAAACCAg                                                                                <  2:941516/143‑1 (MQ=255)
                                                                  gAATGTCATTAATCAGCCGCGCCAGCCCGCTGCCTAAAGGAAGCGCCAGCACCATTAACACCAGTAAAAACGTGGCGATCAGTAAGAACCCTTGCGCAGCCATCAGAACGCCTCCGCATTGATCAGGGCATAAACCAGATAAc                                                                           <  2:243036/143‑1 (MQ=255)
                                                                   aaTGTCATTAATCAGCCGCGCCAGCCCGCTGCCTAAAGGACGCGCCAGCACCATTAACACCAGTAAAAACGTGGCGATCAGTAAGAACCCTTGCGCAGCCATCAGAACGCCTCCGCATTGATCAGGGCATAAACCAGATAAcc                                                                          <  2:778021/143‑1 (MQ=255)
                                                                       tCATTAATCAGCCGCGCCAGCCCGCTGCCTACAAGACGCGCCAGCACCATTAACACCAGTAAAAACGTGGCGATCAGTAAGAACCCTTGCGCAGCCATCAGAACGCCCGCGCCTTGATCCGGGCACAAACCCGATCACCCAGt                                                                      >  2:495624/1‑143 (MQ=255)
                                                                          ttAATCAGCCGCGCCAGCCCGCTGCCTAAAGGACGCGCCAGCACCCTTAACACCAGTAAAAACGTGGCGATCAGGAAGAACCCTTGCGCAGCCATCAGAACGCCTCCGCATTGATCAGGGCATAAACCAGCTAACCCAGcaat                                                                   >  2:559238/1‑143 (MQ=255)
                                                                          ttAATCAGCCGCGCCAGCCCGCTGCCTAAAGGACGCGCCAGCACCATTAACACCAGTAAAAACGTGGCGATCAGTAAGAACCCTTGCGCAGCCATCAGAACGCCTCCGCATTGATCAGGGCATAAACCAGATAACCAAGtaat                                                                   >  2:78014/1‑143 (MQ=255)
                                                                                 gCCGCGCCAGCCCGCTGCCTAAAGGACGCGCCAGCACCATTAACACCAGTAAAAACGTGGCGATCAGTAAGAACCCTTGCGCAGCCATCAGAACGCCTCCGCATTGATCAGGGCATAAACCAGATAACCCAGTAATAAAAaca                                                            >  2:179057/1‑143 (MQ=255)
                                                                                       ccAGCCCCCTGCCTAAAGGACGCGCCAGCACCATTACCACCAGTAAAAACGTGGCGATCAGTCAGAACCCTTGCGCAGCCATCCAAACGCCTCCGCATTGAGCAGGAACTCAACGAGATAACCCAGTCATaacaaccacaccc                                                      >  2:366626/1‑136 (MQ=255)
                                                                                        ccgCCCCCTGCCTACCGGACGCGCCCGCACCATCAACACCAGTAAAAACGTGGCGATCAGTAAGAACCCTTGCGCAGCCATCAGAACGCCTCCGCATTGATCAGGGCATAAACCAGATAACCCAGTAATAAAAACACCAGCaa                                                     <  1:19738/141‑1 (MQ=255)
                                                                                            ccGCTGCCTAAAGGACGCGCCAGCACCATTAACACCAGTAAAAACGTGGCGATCAGTAAGAACCCTTGCGCAGCCATCAGAACGCCTCCGCATTGATCAGGGCATACACCAGATAACCCAGTAATAAAAACACCAGCAATACg                                                 >  2:621696/1‑143 (MQ=255)
                                                                                                  gcTAAAGGACGCGCCAGCACCATTACCACCAGTAAAAACGTGGCGATCAGTAAGAACCCTTGCGCAGCCATCAGAACGCCTCCGCATTGATCAGGGCATAAACCAGATAACCCAGTAATAAAAACACCAGCAATACGCCGGtt                                           <  2:574681/142‑1 (MQ=255)
                                                                                                  ccTAAAGGACGCGCCAGCACCATTAACACCAGTAAAAACGTGGCGATCAGTAAGAACCCTTGCGCAGCCATCAGAACGCCTCCGCAATGATCAGAGCATAAGCCAGATAACCCAGTAATAAAAACACCAGCCACACCGCGGtt                                           >  2:804449/1‑143 (MQ=255)
                                                                                                    tAAAGGACGCGCCAGCACCATTAACACCAGTAAAAACGTGGCGATCAGTAAGAACCCTTGCGCAGCCATCAGAACGCCTCCGCATTGATCAGGGCATAAACCAGATAACCCAGTAATAAAAACACCAGCAATACGCCGGTTAt                                         <  1:314131/143‑1 (MQ=255)
                                                                                                      caGGACGCGCCAGCACCATCAACACCAGTAAAGACGTGGTGATCTCGATGAGCCCTTGCGCAGCCATCAGAACGCCTCCGCATTGATCAGGGCATAAACCAGATAACCCAGTAATAAAAACACCAGCAATACGCCGGTTATCa                                       <  2:211008/142‑1 (MQ=255)
                                                                                                       atgACGCGCCAGCACCATTAACACCAGTAAAAACGTGGCGATCAGTAAGAACCCTTGCGCAGCCATCAGAACGCCTCCGCATTGATCAGGGCATAAACCAGATAACCCAGTAATAAAATCACCAGCAATACGCCGGTTATCAc                                      <  2:632512/141‑1 (MQ=255)
                                                                                                             cgcCAGCACCATTAACACCAGTAAAAACGTGGCGATCAGTAAGAACCCTTGCGCAGCCATCAGAACGCCTCCGCATTGATCAGGGCATAAACCAGATAACCCAGTAATAAAAACACCAGCAATACGCCGATTATCACGCCTGc                                >  1:358275/1‑143 (MQ=255)
                                                                                                              gcCAGCACCATTACCACGAGCAAAAACGGGGCGATCAGTAAGAACCCTTGCGCAGACATCAGAACGCCTCCGCATTGATCAGGGCATAAACCAGATAACCCAGTAATAAAAACACCAGCAATACGCCGGTTATCACGCCTGCa                               <  2:641871/143‑1 (MQ=255)
                                                                                                              gcCAGCACCATTAACACCAGTAAAAACGTGGCGATCAGTAAGACCCCTTGCGCAGCCATCAGAACGCCTCCGCATTGAGCAGGGCATAAACCAGATAACCCAGTAATAAAAACACCAGCAATACGCCGGTTATCACGCCTGCa                               <  1:88431/143‑1 (MQ=255)
                                                                                                                cAGCACCATTAACCCCAGTAAAAACGTGGCAATCAGTAAGAACCCTTGCGCAGCCATCAGAACGCCTCCGCATTGATCAGGGCATAAACCAGATAACCCAGTAATAAAAACACCAGCAATACGCCGGtt                                           <  1:787102/129‑1 (MQ=255)
                                                                                                                cAGCACCATTAACACCAGTAAAAACGTGGCAATCAGTAAGAACCCTTGCGCAGCCATCAGAACGCCTCCGCATTGATCAGGGCATAAACCAGATAACCCAGTAATAAAAACACCAGCAATACGCCGGtt                                           >  2:787102/1‑129 (MQ=255)
                                                                                                                cAGCACCATTAACACCAGTAAAAACGTGGCAATCAGTAAGAACCCTTGCGCAGCCATCAGAACGCCTCCGCATTGATCAGGGCATAAACCAGATAACCCAGTAATAAAAACACCAGCAATACGCCGGtt                                           >  2:787169/1‑129 (MQ=255)
                                                                                                                cAGCACCATTAACACCAGTAAAAACGTGGCAATCAGTAAGAACCCTTGCGCAGCCATCAGAACGCCTCCGCATTGATCAGGGCATAAACCAGATAACCCAGTAATAAAAACACCAGCAATACGCCGGtt                                           <  1:787169/129‑1 (MQ=255)
                                                                                                                   cACCATTAACACCAGTAAAAACGTGGCGATCAGTAAGAACCCTTGCGCAGCCATCAGAACGCCTCCGCATTGATCAGGGCATAAACCAGATAACCCAGTAATAAAAACACCAGCAATACGCCGGTGATCACGCCTGCACTccc                          >  1:83242/1‑141 (MQ=255)
                                                                                                                               cAGTAAAAACGTGGCGATCAGTAAGAACCCTTGCGCAGCCATCAGAACGCCTCCGCATTGATCAGGGCATAAACCAGATAACCCAGTAATAAAAACACCAGCAATACGCCGGTTATCACGCCTGCACTCACAGTGCACCTCCa              <  2:648516/143‑1 (MQ=255)
                                                                                                                                 agcaaaaCGGGGCGATCAGTAAGAACCCTTGCGCAGCCATCAGAACGCCTCCGCATTGATCAGGGCATAAACCAGATAACCCAGTAATAAAAACACCAGCAATACGCCGGTTATCACGCCTGCACTCACAGTGCACCTCCAGt            <  2:51722/140‑1 (MQ=255)
                                                                                                                                           ggCCATCAGTAAGGCCCCTTGCGCAGCCATCAGAACGCCTCCGCATTGATCAGGGCATAAACCAGATAACCCAGTAATAAAAACACCAGCAATACGCCGGTTATCACGCCTGCACTCACAGTGCACCTCCAGTGGCCTAAAAg  <  2:424623/143‑1 (MQ=255)
                                                                                                                                              |                                                                                                                                           
TCTCACGGTCAGAGACGCCAAGTGCGCGAAAAAGTACGCGCTCAACGCCCGTTGTACCGGGAAGAGGAATGTCATTAATCAGCCGCGCCAGCCCGCTGCCTAAAGGACGCGCCAGCACCATTAACACCAGTAAAAACGTGGCGATCAGTAAGAACCCTTGCGCAGCCATCAGAACGCCTCCGCATTGATCAGGGCATAAACCAGATAACCCAGTAATAAAAACACCAGCAATACGCCGGTTATCACGCCTGCACTCACAGTGCACCTCCAGTGGCCTAAAAG  >  NC_000913/728564‑728845

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑