Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
MC JC	NC_000913	3,815,859	Δ82 bp		[rph]	[rph]

Missing coverage evidence...
			seq id	start	end	size	←reads	reads→	gene	description
*	*	÷	NC_000913	3815859	3815940	82	30 [0]	[0] 29	[rph]	[rph]

New junction evidence
		seq id	position	reads (cov)	reads (cov)	score	skew	freq	annotation	gene	product
*	?	NC_000913	= 3815858	0 (0.000)	27 (0.670)	24/260	0.7	100%	intergenic (‑90/+5)	pyrE/rph	orotate phosphoribosyltransferase/truncated RNase PH
	?	NC_000913	3815941 =	0 (0.000)					coding (609/687 nt)	rph	truncated RNase PH

CAAAAAAAAGGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCGATTCCCCTCGGGCCAGAGCCAACAAGATGAGTAGCTCTTCATGGGTGAACGGCTCGCCTTCTGCCGTCCCCTGCACTTCAATGATGCGCCCGTCTTCGGTCATCACTACGTTCATGTCGGTCTCTGCGGCAGAGTCTTCAACGTATTCCAGATCGCAAACCGCTTCGCCGTTCACAATTCCGACAGAAACTGCGGCTACCATCCCT  >  NC_000913/3815800‑3816082                                                                                                                                              |                                                                                                                                              cacctgcttgctaagcgcaaattcaataaactggcgctgatatggtttcatgccttcgctcctcatcttacttttctacagacaaaaaaaaggcgactcatcagtcgccttaaaaatcagtttgccagcgccgccttctgcCg                                                                                                                                              >  1:248249‑M2/142‑143 (MQ=255) cacctgcttgctaagcgcaaattcaataaactggcgctgatatggtttcatgccttcgctcctcatcttacttttctacagacaaaaaaaaggcgactcatcagtcgccttaaaaatcagtttgccagcgccgccttctgcCg                                                                                                                                              >  2:143514‑M2/142‑143 (MQ=255)    ctgcttgctaagcgcaaattcaataaactggcgctgatatggtttcatgccttcgctcctcatcttacttttctacagacaaaaaaaaggcgactcatcagtcgccttaaaaatccgtttgccagcgccgcctcctgcCGTcc                                                                                                                                           >  1:565364‑M2/139‑143 (MQ=255)       cttgctaagcgcaaattcaataaactggcgctgatatggtttcatgccttcgctcctcatcttacttttctacagacaaaaaaaaggcgactcatcagtcgccttaaaaatcagtttgccagcgccgccttctgcCGTCCCCt                                                                                                                                        >  1:708900‑M2/136‑143 (MQ=255)          gctaagcgcaaattcaataaactgtcgctgatatggtttcatgccttcgctcctcatcttacttttctacagacaaaaaaaaggcgactcatcagtcgccttaaaaatcagtttgccagcgccgccttctgcCGTCCCCTGCa                                                                                                                                     <  2:405353‑M2/11‑1 (MQ=255)            taagcgcaaattcaataaccttcctctgatatggtttcatgccttcgctcctcatcttacttttctactgacaaaaaaaaggcgactcatcagtcgccttaaaaatcagtttgccagcgccgccttctgcCGTCCCCTGCACt                                                                                                                                   <  1:470901‑M2/13‑1 (MQ=255)                ggcaaattcaataaactggcgctgatatggtgtcatgccttcgctcctcatcttacttttctacagacaaaaaaaaggcgactcatcagtcgccttaaaaatcagtttgccagcgccgccttctgcCGTCCCCTGCACTTCaa                                                                                                                               <  2:6378‑M2/17‑1 (MQ=255)                 gcaaattcaataaactggcgctgatatggtttcatgccttcgctcctcatcttacttttctacagacaaaaaaaaggcgactcatcagtcgccttaaaaatcagtttgccagcgccgccttctgcCGTCCCCTGCACTTCAat                                                                                                                              >  1:647316‑M2/126‑143 (MQ=255)                         aataaactggcgctgatatggtttcatgccttcgctcctcatcttacttttctacagacaaaaaaaaggcgactcatcagtcgccttaaaaatcagtttgccagcgccgccttctgcCGTCCCCTGCACTTCCATGGTGCGcc                                                                                                                      >  1:760024‑M2/118‑143 (MQ=255)                               ctgtcgctgatatggtttcatgccttcgctcctcatcttacttttctacagacaaaaaaaaggcgactcatcagtcgccttaaaaatcagtttgccagcgccgccttctgcCGTCCCCTGCACTTCAATGATGCGCCCGTCtt                                                                                                                <  1:360582‑M2/32‑1 (MQ=255)                                  gcgctgagatggtttcatgccttcgctcctcatcttacttttctacagacaaaaaaaaggcgactcatcagtcgccttaaaaatcagtttgccagcgccgccttctgcCGTCCCCTGCACTTCAATGATGCGCCCGTCTTCgg                                                                                                             <  1:440591‑M2/35‑1 (MQ=255)                                             gtttcatgccttcgctcctcatcttacttttctacagacaaaaaaaaggcgactcatcagtcgccttaaaaatcagtttgccagcgccgccttctgcCGTCCCCTGCACTTCAATGATGCGCCCGTCTTCGGTCATCACTACg                                                                                                  <  2:157344‑M2/46‑1 (MQ=255)                                                                 atcttacttttctacagacaaaaaaaaggcgactcatcagtcgccttaaaaatcagttttccagcgccgccttctgcCGTCCCCTGCACTTCAATGATGCGCCCGTCTTCGGCCCTCTCTCCGTTCATGTCGGTCTCTGcgga                                                                              >  1:74462‑M2/78‑142 (MQ=255)                                                                       cttttctacaaataaataaaaggcgactcatcagtcgccttaaaaatcattttgccagcgccgccttctgcCGTCCCCTGCACTTCAATGATGCGCCCGTCTTCGGTCATCACTACGTTCATGTCGGTCTCTGCGGCAGAGTc                                                                        <  2:123355‑M2/72‑1 (MQ=255)                                                                                agacaaaaaaaaggcgactcatcagtcgccttaaaaatcagtttgccagcgccgccttctgcCGTCCCCTGCACATCAATGATGCGCCCGTCTTCGGTCATCACTACGTTCATGTCGGTCTCTGCGGCAGAGTCCGCAACGTa                                                               >  2:440519‑M2/63‑143 (MQ=255)                                                                                    aaaaaaaaggcgactcatcagtcgccttaaaaatcagtttgccagcgccgccttctgcCGTCCCCTGCACTTCAATGATGCGCCCGTCTTCGGTCATCACTACGTTCATGTCGGTCTCTGCGGCAGAGTCTTCAACGTATTcc                                                           >  1:564252‑M2/59‑143 (MQ=255)                                                                                     aaaaaaaggcgactcatcagtcgccttaaaaatcagtttgccagcgccgccttctgcCGTGCCCAGCCATTCAGTTATGGGGCTTTCTTCGGTCATCACTACGTTCATGTCGGTCTCTGCGGCAGAGTCTTCAACGTATTCCa                                                          <  1:185511‑M2/86‑1 (MQ=255)                                                                                        aaaatgcgactcatcagtcgccttaaaaatcagtttgccagcgccgccttctgcCGTCCCCTGCACTTCAATGATGCGCCCGTCTTCGGTCATCACTACGTTCATGTCGGTCTCTGCGGCAGAGTCTTCAACGTATTCCAGAt                                                       <  2:677997‑M2/89‑1 (MQ=255)                                                                                        aaaaggcgactcatcagtcgccttaaaaatcagtttgccagcgccgccttctgcCGTCCCCTGCACTTCAATGATGCGCCCGTCTTCGGTCATCACTACGTTCATGTCGGTCTCTGCGGCAGAGTCTTCAACGTATTCCAGAt                                                       <  2:248249‑M2/89‑1 (MQ=255)                                                                                               gactcagcagtcgccttaaaaatcagtttgccagcgccgccttctgcCGTCCCCTGCACTTCAATGATGCGCCCGTCTTCGGTCATCACTACGTTCATGTCGGTCTCTGCGGCAGAGTCTTCAACGTATTCCAGATCGCAAAc                                                <  1:318775‑M2/96‑1 (MQ=255)                                                                                                    gtcagtcgccttaacaatcggtttgccagcgccgcctgctgcCGTCCCCTGCACTTCAATGCTGCGCCCGTCTTCGGTCATCACTACGTTCATGTCGGTCTCTGCGGCAGAGTCTTCAACGTATTCCAGATCGCAAACCGCtt                                           <  2:789335‑M2/101‑1 (MQ=255)                                                                                                          cgccttaaaaatcagtttgccagcgccgccttctgcCGTCCCCTGCACTTCAATGATGCGCCCGTCTTCGGTCATCACTACGTTCATGTCGGTCTCTGCGGCAGAGTCTTCAACGTATTCCAGATCGCAAACCGCTTCGCCGt                                     <  1:441393‑M2/107‑1 (MQ=255)                                                                                                           gccttaaaactccgtttcccagcgccgccttctgcCGTCCCCTGCACTTCAATGATGCGCCCGTCTTCGGTCATCACTACGTTCATGTCGGTCTCTGCGGCAGAGTCTTCAACGTATTCCAGATCGCAAACCGCTTCGCCGtt                                    <  1:202838‑M2/108‑1 (MQ=255)                                                                                                           gccttaaaaatcagtttgccagcgccgccttctgcCGTCCCCTGCACTTCAATGATGCGCCCGTCTTCGGTCATCACTACGTTCATGTCGGTCTCTGCGGCAGAGTCTTCAACGTATTCCAGATCGCAAACCGCTTCGCCGtt                                    >  2:242481‑M2/36‑143 (MQ=255)                                                                                                           gcctgaaaaagcagtttgccagcgccgccttctgcCGTCCCCTGCACTTCAATGATGCGCCCGTCTTCGGTCATCACTACGTTCATGTCGGTCTCTGCGGCAGAGTCTTCAACGTATTCCAGATCGCAAACCGCTTCGCCGtt                                    <  1:770806‑M2/108‑1 (MQ=255)                                                                                                                       tgtttgcccgcgcagcctcctgcCGTCCTCTGAACTTCAAGGCTGCGCCCGTCTTCGGTCATCACTACGTTCATGTCGGTCTCTGCGGCAGAGTCTTCAACGTATTCCAGATCGCAAACCGCTTCGCCGTTCACAATTCCGAc                        <  2:615315‑M2/120‑1 (MQ=255)                                                                                                                                   ccgccttctgcCGTCCCCTGCACTTCAATGATGCGCCCGTCTTCGGTCATCACTACGTTCATGTCGGTCTCTGCGGCAGAGTCTTCAACGTATTCCAGATCGCAACCCGCTTCGCCGTTCACAATTCGGCCAGAACCTGCGGc            >  1:384816‑M2/12‑143 (MQ=255)                                                                                                                                             cCGTCCCCTGCACTTCAATGATGCGCCCGTCTTCGGTCATCACTCCGTTCATGTCGGTCTCTGCGGCAGAGTCTTCAACGTATTCCAGATTGCAAACCGCTTCGCCGTTCACAATTCCGACAGAAACTGCGGCTACCATCCCt  <  2:62639‑M2/142‑1 (MQ=255)                                                                                                                                             cCGTCCCCTGCACTTCAATGATGCGCCCGTCTTCGGTCATCACTACGTTCATGTCGGTCTCTGCGGCAGAGTCTTCACCGTATTCCAGATCGCAAACCGCTTCGCCGTTCACAATTCCGACAGAAACTGCGGCTACCACCCCt  >  2:150111‑M2/2‑143 (MQ=255)                                                                                                                                              |                                                                                                                                              CAAAAAAAAGGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCGATTCCCCTCGGGCCAGAGCCAACAAGATGAGTAGCTCTTCATGGGTGAACGGCTCGCCTTCTGCCGTCCCCTGCACTTCAATGATGCGCCCGTCTTCGGTCATCACTACGTTCATGTCGGTCTCTGCGGCAGAGTCTTCAACGTATTCCAGATCGCAAACCGCTTCGCCGTTCACAATTCCGACAGAAACTGCGGCTACCATCCCT  >  NC_000913/3815800‑3816082

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑