| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 2,661,969 | C→G | V55L (GTT→CTT) | iscR ← | DNA‑binding transcriptional dual regulator IscR |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 2,661,969 | 0 | C | G | 97.9% | 139.2 / NA | 47 | V55L (GTT→CTT) | iscR | DNA‑binding transcriptional dual regulator IscR |
| Reads supporting (aligned to +/- strand): ref base C (0/0); major base G (23/23); minor base A (0/1); total (23/24) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
GCCGCCCTGGCAGCCGCCTTTACCCTGACAACGGGTGGCATCTACAGATTCGTCAACGGCGCTAATTACTTCGCCAACGGCGATGCTGCTGGCATCTTTGCCTAACAGATAACCACCGCCTGGTCCACGTACGCTGGAAACCAGACCATTTTTACGCAGACGG‑GAAAACAGTTGTTCCAGATAAGAAAGGGAAATTCCCTGACGTTCGGAAATATCAGCCAACGGTACCGGGCCCGCTTCAGAGTTGAGCGCAACGTCAAGCATTGCGGTCACGG > NC_000913/2661829‑2662103 | gccgccCTGGCAGCCGCCTTTACCCTGACAACGGGTGGCATCTACAGATTCGTCAACGGCGCTAATTACTTCGCCAACGGCGATGCTGCTGGCATCTTTGCCTAACAGATAACCACCGCCTGGTCCACGTACGCTGGAAAGCa < 2:10893/143‑1 (MQ=255)gccgcaCTGGCAGCAGGCTTTACCCTGACAACGGGTGGCATCTACAGATTCGGCAACGGCGCTAATTACTTCGCCAACGGCGATGCTGCTGGCATCTTTGCCTAACAGATAACCACCGCCTGGTCCACGTACGCTGGAAAGCa < 1:243591/143‑1 (MQ=255) gccCTGGCAGCCGCCTTTACCCTGACAACGGGTGGCATCTACAGATTCGTCAACGGCGCTAATTACTTCGCCAACGGCGATGCTGCTGGCATCTTTGCCTAACAGATAACCACCGCCTGGTCGACGTACGCTGGAAAGCAGAc > 1:161006/1‑143 (MQ=255) cccTGGCAGCCGCCTTTACCCTGACAACGGGTGGCATCTACAGATTCGTCAACAGCGCTAATTACTTCGCCAACGGCGATGCTGCTGGCATCTTTGCCTAACAGATAACCACAGCCTGGTCGAGGTACCCTGGAAAGGAGAcc > 2:410828/1‑143 (MQ=255) aGCCGCCTTTACCCTGACAACGGGTGGCATCTACAGATTCGTCAACGGCGCTAATTACTTCGCCAACGGCGATGCTGCTGGCATCTTTGCCTAACAGATAACCACCTCCTGGTCCACGTACGCTGGAAAGCAGACCATTTTTa < 1:517417/143‑1 (MQ=255) tccgccTTTACCCTGACAACGGGTGGCATCTACAGATTCGTCGACGGCGCTAATTACCTCGCCAACGGCGATGCTGCTGGCATCTTTGCCTAACAAATAACCACCGCCTGGTCCACGTACGCTGGAAAGCAGACCATTTTTAc < 1:413497/142‑1 (MQ=255) gccgctTTTACTCGGACAACGGGTGGCACATACAGATTCGTCAACGGCGCTAATTACTTCGCCAACGGCGATGCTGCTGGCATCTTTGCCTAACAGATAACCACCGCCTGGTCCACGTACGCTGGAAAGCAGACCATTTTTAc < 1:48047/143‑1 (MQ=255) ccgccTTTACCCTGACAACGGGTGGCATCTACAGATTCGTCAACGGCGCTAATTACTTCGCCAACGGCGATGCTGCTGGCATCTTTGCCTAACAGATAACCACCGCCTGGTCCACGTACGCTGGAAAGCAGACCATTTTTACg > 1:160232/1‑143 (MQ=255) ccttGACAATGGGTGGCTTCTACAGATTCGTCAACGGCGCGAATTACTGCGCCAACGGCGATGCTGCTGGCATCTTTGCCTAACAGATAACCACCGCCTGGTCCACGTACGCTGGAAAGCAGACCATTTTTACGCAGACGG‑Ga < 1:612829/140‑1 (MQ=255) cccTGACAACGGGTGGCATCTACAGATTCGTCAACGGCGCTAATTACTTCGCCAACGGCGATGCTGCTGGCATCTTTGCCTAACAGATAACCACCGCCTGGTCCACGTACGCTGGAAAGCAGACCATTTTTACGCAGACGG‑Ga < 1:787404/143‑1 (MQ=255) aCGGGTGGCATCTACAGATTCGTCAACGGCGCTAATTACTTCGCCAACGGCGATGCTGCTGGCATCTTTGCCTAACAGATAACCACCGCCTGGTCCACGTACGCTGGAAAGCAGACCATTTTTACGCAGACGG‑GAAAACAgtt > 2:646855/1‑143 (MQ=255) aCGGGTGGCATCTACAGATTCGTCAACGGCGCTAATTACTTCGCCAACGGCGATGCTGCTGGCATCTTTGCCCAACAGATAACCACCGCCTGGTCCACGTACGCTGGAAAGCAGACCATTTTTACGCAGACGG‑GAAAACAgtt < 2:336751/143‑1 (MQ=255) gTGGCATCTACAGATTCGTCAACGGCGCTAATTACTTCGCCAACGGCGATGCTGCTGGCATCTTTGCCTAACAGATAACCACCGCCTGGTCCACGTACGCTGGAAAGCAGACCATTTTTACGCAGACGG‑GAAAACAGTTGTTc > 2:100234/1‑143 (MQ=255) tCTACAGACTCGTCAACGGCGCTAATTACTTCGCCAACGGCGATGCTGCTGGCATCTTTGCCTAACAGATAACCACCGCCTGGTCCACGTACGCTGGAAAGCAGACCATTTTTACGCAGACGG‑GAAAACAGTTGTTCCAGATa < 1:304560/143‑1 (MQ=255) cTACAGATTCGTCAACGGCGCTAATTACTTCGCCAACGGCGATGCTGCTGGCATCTTTGCCTAACAGATAACCACCGCCTGGTCCACGTACGCTGGAAAGCAGACCATTTTTACGCAGACGG‑GAAAACAGTTGTTCCAGATaa < 1:250009/143‑1 (MQ=255) tACAGATTCGTCAACGGCGCTAATTACTTCGCCAACGGCGATGCTGCTGGCATCTTTGCCTAACAGATAACCACCGCCTGGTAGCCGAAACGTGGAGAGGAGACCATTTTTACGCAGACGG‑GAAAACCGTTGTTCCAGATAAg > 1:368927/1‑143 (MQ=255) gTCAACGGCGCTAATTACTTCACCAACGGCGATGCTGCTGGCATCTTTGCCTAACAGATAACCACCGCCTGGTCCACGTACGCTGGAAAGCAGACCATTTTTACGCAGACGG‑GAACACAGCTGGTCCAGATAAGAAAGAGaac > 2:122766/1‑142 (MQ=255) aCGGCGCTAATTACTTCGCCAACGGCGATGCTGCTGGCATCTTTGCCTAACAGATAACCACCGCCTGGTCCACGTACGCTGGAAAGCAGACCATTTTTACGCAGACGG‑GAAAACAGTTGTTCCAGATAAGAAAGGGAAATTcc < 1:646855/143‑1 (MQ=255) gcTAATTACTTCGCCAACGGCGATGCTGCTGGCATCTTTGCCTAACGGATAACCACCGCCTGGTCCACGTACGCTGGAAAGCAGACCATTTTTACGCAGACGG‑GAAAACAGTTGTTCCAGATAAGAAAGGGAAATTCCCTGAc < 2:747800/143‑1 (MQ=255) tAATTACTTCGCCAACGGCGATGCTGCTGGCATCTTTGCCTAACAGATAACCACCGCCTGGTCCACGTACGCTGGAAAGCAGACCATTTTTACGCAGACGG‑GAAAACAGTTGTTCCAGATAAGAAAGGGAAATGCCCTGACGt > 1:129839/1‑143 (MQ=255) ttCGCCAACGGCGATGCTGCTGGCATCTTTGCCTAACAGATAACCACCGCCTGGTCCACGTACGCTGGAAAGCAGACCATTTTTACGCAGACGG‑GAAAACAGTTGTTCCAGATAAGAAAGGGAAATTCCCTGACGTTCGGaaa > 1:275390/1‑143 (MQ=255) tgcGCCACCGGCGCGGCTGCTGGCATCTTCGCCTAGCAGATAACCTCCGCCTGGTCCACGTACGATGGAAAGCAGACCATTTTTACGCAGACGG‑CAAAACAGTTGTTCCAGATAAGAACGGGAAATTCCCTGACGTTCGGaaa < 2:322179/141‑1 (MQ=255) cGCCAACGGCGATGCTGCTGGCATCTTTGCCTAACAGATAACCACCGCCTGGTCCACGTACGCTGGAAAGCAGACCATTTTTACGCAGACGG‑GAAAACAGTCGTTCCAGATAACAACGGGAAATCCCCTGACGCTCGGAAatc > 2:216041/1‑142 (MQ=255) aaaCGGCGATGCTGCTGGCATCTTTGCCTAACAGATAACCACCGCCTGGTCCACGTACGCTGGAAAGCAGACCAGTTTTACGCAGACGG‑GAAAACAGTTGTTCCAGATAAGAAAGGGAAATTCCCTGACGTTCGGAAATATCa < 2:94068/142‑1 (MQ=255) gCGACGCTGCTGGCATATTTGCCTAACAGATAACCACCGCCTGGTCCACGTACGCTGGAAAGCAGACCATTTTTACGCAGACGG‑GAAAACAGTTGTTCCAGATAAGAAAGGGAAATTCCCTGACGTTCGGAAATATCAGCCaa < 1:245414/143‑1 (MQ=255) atcctgctgGCATCTTTGCCTAACAGATAACCCCCGCCTGGTCCACGTACGCTGGAAAGCAGACCATTTTTACGCAGACGG‑GAAAACAGTTGTTCCAGATAAGAAAGGGAAATTCCCTGACGTTCGGAAATATCAGCCAACgg < 1:410828/140‑1 (MQ=255) tgctgGCATCTTTGCCTAACAGATAACCACCGCCTGGTCCACGTACGCTGGAAAGCAGACCATTTTTACGCAGACGG‑GAAAACAGTTGTTCCAGATAAGAAAGGGAAATTCCCTGACGTTCGGAAATATCAGCCAACGGGAcc > 2:4809/1‑143 (MQ=255) gCATCTTTGCCTAACAGATAACCACCGCCTGGTCCACGTACGCTGGAAAGCAGACCATTTTTACGCAGACGG‑GAAAACAGTTGTTCCAGATAAGAAAGGGAAATTCCCTGACGTTCGGAAATATCAGCCAACGGTACCGGGcc < 1:465781/143‑1 (MQ=255) tttGCCTAACAGATAACCACCGCCTGGTCCACGTACGCTGGAAAGCAGACCATTTTTACGCAGACGG‑GAAAACAGTTGTTCCAGATAAGAAAGGGAAATTCCCTGACGTTCGGAAATATCAGCCAACGGTACCGGGCCCGCtt < 1:523876/143‑1 (MQ=255) cTAACAGATAACCACCGCCTGGTCCACGTACGCTGGAAAGCAGAGCATTTTTACGCAGACGG‑GAAAACAGTTGTTCCAGATAAGAAAGGGAAATTCCCTGACGTTCGGAAATATCAGCCAACGGTACCGGGCCCGCTTCagag > 2:699044/1‑143 (MQ=255) cAGATAACCGCGGCCTGGTCCACGTACGCTGGAAAGCAGACCATTTTTACGCAGACGG‑GAAAACAGTTGTTCCAGATAAGAAAGGGAAATTCCCTGACGTTCGGAAATATCAGCCAACGGTACCGGGCCCGCTTCAGAGTTGa < 1:601650/143‑1 (MQ=255) aGATAACCACCGCCTGGTCCACGTACGCTGGAAAGCAGACCATTTTTACGCAGACGG‑GAAAACAGTTGTTCCAGATAAGAAAGGGAAATTCCCTGACGTTCGGAAATATCAGCCAACGGTACCGGGCCCGCGTCAGAGGTgcg > 1:343881/1‑141 (MQ=255) aTAACCACCGCCTGGTCCACGTACGCTGGAAAGCAGACCATTTTTACGCAGACGG‑GAAAACAGTTGTTCCAGATAAGAAAGGGAAATTCCCTGACGTTCGGAAATATCAGCCAACGGTA‑CAGGCCCGCTTCAGAGCTGAgcgc > 1:320802/1‑143 (MQ=255) tAACCACCGCCTGGTCCCCGTACGCTGGAAAGCAGACCATTTTTACGCAGACGG‑GAAAACAGTTGTTCCAGATAAGAAAGGGAAATTCCCTGACGTTCGGAAATATCAGCCAACGGTACCGGGCCCGCtt < 2:224122/130‑1 (MQ=255) tAACCACCGCCTGGTCCACGTACGCTGGAAAGCAGACCATTTTTACGCAGACGG‑GAAAACAGTTGTTCCAGATAAGAAAGGGAAATTCCCTGACGTTCGGAAATATCAGCCAACGGTACCGGGCCCGCtt > 1:224122/1‑130 (MQ=255) aaCCACCGCCTGGTCCACGTACGCTGGAAAGCAGACCATTTTTACGCAGACGG‑GAAAACAGTTGTTCCAGATAAGAAAGGGAAATTCCCTGACGTTCGGAAATATCAGCCAACGGTACCGGGCCCGCTTCAGAGTTGAgcgga > 1:95569/1‑141 (MQ=255) aaCCACCGCCTGGTCCACGTACGCTGGAAAGCAGACCATTTTTACGCAGACGG‑GAAAACAGTTGTTCCAGATAAGAAAGGGAAATTCCCTGACGTTCGGAAATATCAGCCAACGGTACCGGGCCCGCCTCAGAGTTGAGCGCa > 2:120219/1‑143 (MQ=255) aaCCACCGCCCGGTCCACGCACGCTGGAAAACAGACCATTTTTACGCAGACGG‑GAAAACAGTTGTTCCAGATAAGAAATGGAAATTCCCTGACGTTCGGAAATATCAGCCAACGGTACCGGGCCCGCTTCAGAGTTGAGCGCa < 2:449725/143‑1 (MQ=255) ccaccGCCTGGTCCACGTACGCTGGAAAGCAGACCATTTATACGCAGACGG‑GAAAACCGTTGTTCCAGATAAGAAAGGGAAATTCCCTGACGTTCGGAAATATCAGCCAACGGTACCGGGGCCGCTCCAGCGTTGAACGCAAc > 2:391622/1‑143 (MQ=255) caccGCCTGGTCCACGTACGCTGGAAAGCAGACCATTTTTACGCAGACGG‑GAAAACAGTTGTTCCAGATAAGAAAGGGAAATTCCCTGCCGTGCGGACATAACAGCCAACGGTACCGGGCCCGCTTCAGAGCAAAGCGCaaag > 2:446323/1‑141 (MQ=255) accGTCTGGTCCGCGTCCGCTGGAAAGCAGACCATTTTTACGGAGTTGGTTGTTTTAGCTG‑TCCAGATCAGAGAGCGAAATTCCCTGACGTTCCGAAATATCAGCCAACGGTACCGGGCCCGCTTCAGAGTTGAGCGCAACGt < 2:778070/143‑1 (MQ=255) ggTCCACGTACGCTGGAAAGCAGACCATTGTTACGCAGACGG‑GAAAACAGTTGTTCCAGATAAGAAAGGGAAATTCCCTGACGTTCGGAAATATCAGCCAACGGTACCGGGCCCGCTTCAGAGTTGAGCGCAACGTCAAGCAt < 1:100234/143‑1 (MQ=255) gTCCACGTACGCTGGAAAGCAGACCATTTTTACGCAGACGG‑GAAAACAGTTGTTCCAGATAAGAAAGGGAAATTCCCTGACGTTCGGAAATATCAGCCAACGGTACCGGGCCCGCTTCCGTGTTGAGCGCGACGTTAAGCAtt > 1:92825/1‑143 (MQ=255) tCCACGTACGCTGGAAAGCAGACCATTTTTACGCAGACGG‑GAAAACAGTTGTTCCAGATAAGAAAGGGAAATTCCCTGACGTTCGGAAATATCAGCCAACGGTACCGGGCCCGCTTCAGAGTTGAGCGCAACGTCAAGCATTg > 1:159939/1‑143 (MQ=255) cACGTACGCTGGAAAGCAGACCATTTTTACGCAGACGG‑GAAAACAGTTGTTCCAGATAAGAAAGGGAAATTCCCTGACGTTCGGAAATATCAGCCAACGGTCCCGGGCCCGCTTCAGAGTTGCGCGCAACGTCAAGcctcgcc > 2:85608/1‑137 (MQ=255) aCGCTGGAAAGCAGACCATTTTTACGCAGACGG‑GAAAACAGTTGTTCCAGATAAGAAAGGGAAATTCCCTGACGTTCGGAAATATCAGCCAACGGTACCGGGCCCGCTTCAGAGTTGAGCGCAACGTCAAGCATTGCGGTCAc > 1:659640/1‑143 (MQ=255) gCTGGTAAGCAGACCATGTTTACGCAGACGG‑GAAAACAGTGGTTCCAGCTAAGAAAGGGAAATTCCCTGACGTTCGGAAATAGCAGCCAACGGTACCGGGCCCGCTTCAGAGTTGAGCGCAACGTCAAGCATTGCGGTCACgg < 2:128562/143‑1 (MQ=255) | GCCGCCCTGGCAGCCGCCTTTACCCTGACAACGGGTGGCATCTACAGATTCGTCAACGGCGCTAATTACTTCGCCAACGGCGATGCTGCTGGCATCTTTGCCTAACAGATAACCACCGCCTGGTCCACGTACGCTGGAAACCAGACCATTTTTACGCAGACGG‑GAAAACAGTTGTTCCAGATAAGAAAGGGAAATTCCCTGACGTTCGGAAATATCAGCCAACGGTACCGGGCCCGCTTCAGAGTTGAGCGCAACGTCAAGCATTGCGGTCACGG > NC_000913/2661829‑2662103 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |