Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,041,632	T→A	V107E (GTG→GAG)	ygfZ →	folate‑binding protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,041,632	0	T	A	100.0%	118.8 / NA	43	V107E (GTG→GAG)	ygfZ	folate‑binding protein
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base A (18/25);  total (18/25)

GCCCATTGCGACGCCAAAGGTAAAATGTGGAGCAATTTACGTCTGTTCCGCGACGGCGATGGCTTTGCATGGATTGAACGGCGCAGCGTGCGTGAACCGCAGCTGACTGAACTGAAAAAATATGCGGTATTCTCTAAAGTGACCATCGCGCCAGACGACGAGCGTGTGCTGCTTGGTGTTGCCGGTTTTCAGGCGCGCGCCGCGCTGGCAAATCTCTTTAGCGAACTGCCTTCGAAAGAAAAACAGGTAGTCAAAGAAGGCGCGACCACTTTGCTATGG  >  NC_000913/3041493‑3041771                                                                                                                                            |                                                                                                                                            gccCATTGCGACGCCAAAGGAAAAATGTGGAGCAATTTACGTCTGTTCCGCGCCGGCGATGGCTTTGCATGGATTGAACGGCGCAGCGTGCGTGAACCGCAGCTGACTGAACTGAAAAAATATGCGGTATTCTCTAAAGAGAc                                                                                                                                          <  1:411184/143‑1 (MQ=255)  cccATTGCGACGCCAAAGGTAAAATGTGGAGCAATTTACGTCTGTTCCGCGACGGCGATGGCTTTGCATGGATTGAACGGCGCAGCGTGCGTGAACCGCAGCTGACTGAACTGAAAAAATATGCGGTATTCTATATAGAGAcc                                                                                                                                         <  2:745171/143‑1 (MQ=255)          gACGCCAAAGGTAAAATGTGGAGCAATTTACGTCTGTTCCGCGACGGCGATGGCTTGGCAGGGATCGAAAGCTCCAGCGTGCGTGAACCGCAGCTGACTGAACTGAAAAAATATGCGGTATTCTCTAAAGAGGCCATCGCGcc                                                                                                                                 >  2:696958/1‑143 (MQ=255)                aaaGGTAAAATGTGGAGCAATTTACGTCTGTTCCGCGACGGCGATGGCTTTGCATGGATTGAACCGCGCAGCGTGCGTGAACCGCAGCTGACTGAACTGAAAAAATGTGCGGTATTCTCTGAAGAGACCATCGCGCCAgacga                                                                                                                           >  2:656050/1‑143 (MQ=255)                  aGGTAAAATGTTGACCAATTTACGTCTGTTCCGCGACGGCGCTGGCTCTGCATGGATTGAACGGCGCAGCGTGCGTGAACCGCAGCTGACTGAACTGAAAAAATATGCGGTATTCTCTAAAGAGACCATCGCGCCAGacgacg                                                                                                                         <  1:703359/143‑1 (MQ=255)                    gTAAAATGTGGAGCAATTTACGTCTGTTCCGCGACGGCGATGGCTTTGCATGGATTGAACGGCGCAGCGTGCGTGAACCGCAGCTGACTGAACTGAAAAAAGAGGCGGTATTCTCTGAAGAGACCATCGCGCCAGACGACGAg                                                                                                                       >  1:366787/1‑143 (MQ=255)                         aggtgGAGCAATTTACGTCTGTTCCGCGACGGCGATGGCTTTGGATGGAGTGAACGGCGCAGCGTGCGTGAACCGCAGCTGACTGAACTGAAAAAATATGCGGTATTCTCTAAAGAGACCATCGCGCCAGACGACGAGCgtgt                                                                                                                  <  1:713144/141‑1 (MQ=255)                         aTGTGGAGCAATTTACGTCTGTTCCGCGACGGCGATGGCTTTGCATGGATTGAACGGCGCAGCGTGCGTGAACCGCAGCTGACTGAACTGAAAAAATATGCGGTATTCTCTAAAGAGACCATCGCGCCAGACGACGAGCgtgt                                                                                                                  <  1:683681/143‑1 (MQ=255)                          tgtgGTGCAAGGTACGTCTGTTCCGCGACGGCGATGGCTTTGCATGGATTGACCGGCGCAGCGTGCGTGAACCGCAGCTGACTGAACTGAAAAAATATGCGGTATTCTCTAAAGAGACCATCGCGCCAGACGACGAGCGtgtg                                                                                                                 <  1:658934/143‑1 (MQ=255)                              gAGCAATTTACGTCTGTTCCGCGACGGCGATGGCTTTGCATGGATTGAACGGCGCAGCGTGCGTGAACCGCAGCTGACTGAACTGAAAAGGTGCGCGGTATGCTCTAAAGAGGCCATCGCGCCGGACGAGGAGAGTGtgcggc                                                                                                             >  2:461838/1‑143 (MQ=255)                                           gtGTTCGGCGACGGCGATGGCTTTGCATGGATTGAGTGGCGCAACATGCGTGAACCGCAGCTGACTGAACTGAAAAAAGATGCGGTATTCTCGAAAGAGACCATCGCGCCAGACGACGAGCGTGTGCTGCTTGGTGTTGCCgg                                                                                                <  2:353246/142‑1 (MQ=255)                                           cTGTTCCGCGACGGCGATGGCTTTGCATGGATTGAACGGCGCAGCGTGCGTGAACCGCAGCTGACTGAACTGAAAAAATATGCGGTATTCTCTAAAGAGACCATCGCGCCAGACGACGAGCGTGTGCTGCTTGGTGTTGCCgg                                                                                                >  1:57808/1‑143 (MQ=255)                                                  gcgACGGCGATGGCTTTGCATGGATTGAACGGCGCAGCGTGCGTGAACCGCAGCTGACTGAACTGAAAAAATATGCGGTATTCTCTAAAGAGACCATCGCGCCAGACGACGAGCGTGTGCTGCTTGGTGTTGCCGGTTTTCAg                                                                                         <  2:673269/143‑1 (MQ=255)                                                   cgACGGCGATGGCTTTGCAGGGATTGAACGGCGAAGCGTGCGTCCAGCGAAGCTTACGTAGCTGTTAAAATAATCGGTATTCTCTAAAGAGACCATCGCGCCAGACGACGAGCGTGTGCTGCTTGGTGTTGCCGGTTTTCAgg                                                                                        <  2:777688/143‑1 (MQ=255)                                                          gATGGCTTTGCATGGATTGAACGGCGCAGCGTGCGTGAACCGCAGCTGACTGAACTGAAAAATTATGCGGTATACTCTAAAAAGACCATCGCGCCAGACGACGAGCGGGTGCGGCTTGGTGTGGCCGGTGTAAAGgcgcgcgc                                                                                 >  2:797683/1‑143 (MQ=255)                                                                 ttGCATGGATTGAACGGCGCAGCGTGCGTGAACCGCAGCTGACTGAACTGAAAAAATATGCGGTATTCTCTAAAGAGACCATCGCGCCAGACGACGAGCGTGTGCTGCTTGGTGTTGCCGGTTTTCAGGCGCGCGCCGCGCTg                                                                          <  1:655427/143‑1 (MQ=255)                                                                 ttGCATAGATTGAAGGGCGCAGCGTGCGTGAACCGCAGCTGACTGAACTGAAAAAATATGCGGTATTCTCTAAAGAGACCATCGCGCCAGACGACGAGCGTGTGCTGCTTGGTGTTGCCGGTTTTCAGGCGCGCGCCGCGCTg                                                                          <  2:365995/143‑1 (MQ=255)                                                                 ttGCAGGGATTGAACGGTGCGGGGTGCGGGAACCGCAGCTGACGGAACTGAAAAAATATGCGGTATTCTCTAAAGAGACCATCGCGCCAGACGACGAGCGTGTGCTGCTTGGTGTTGCCGGTTTTCAGGCGCGCGCCGCGCTg                                                                          <  2:711604/143‑1 (MQ=255)                                                                 gtGCATGGATTGAGCGGCGCAGCGTGCGTGAACCGCAGCTGACTGAACTGAAAAAATATGCGGTATTCTCTAAAGAGACCATCGCGCCAGACGACGAGCGTGTGCTGCTTGGTGTTGCCGGTTTTCAGGCGCGCGCCGCGCTg                                                                          <  2:368655/142‑1 (MQ=255)                                                                  tGCATGGATTGGACGGCGCAGCGTGCGTGAACCGCAGCTGACTGAACTGAAAAAATATGCGGTATTCTCTAAAGAGACCATCGCGCCAGACGACGAGCGTGTGCTGCTTGGTGTTGCCGGTTTTCAGGCGCGCGCCGCGCTgg                                                                         <  2:743453/143‑1 (MQ=255)                                                                       ggATGGAACGGCGCAGCGGGCGTGAACCGCAGCTGACTGAACTGAAAAAATATGCGGTATTCTCTAAAGAGACCATCGCGCCAGACGACGAGCGGGGGCTGCTTGGGGTTGCCGGTTTTCAGGCGCGCGCCGCGCTGGCAAAt                                                                    <  1:130625/143‑1 (MQ=255)                                                                          ttGAACGGCGCAGCGTGCGTGAACCGCAGCTGACTGAACTGAAAAAATATGCGGTATTCTCTAAAGAGACCATCGCGCCAGACGACGAGCGTGTGCTGCTTGGTGTTGCCGGTTTTCAGGCGCGCGCCGCGCTGGCAAAtctc                                                                 >  1:423628/1‑143 (MQ=255)                                                                           tGACCGGCGCAGCGTGCGTGAACCGCAGCTGACTGAACTGAAAAAATATGCGGTATTCTCTAAAGAGACCATCGCGCCAGACGACGAGCGTGTGCTGCTTGGTGTTGCCGGTTTTCAGGCGCGCGCCGCGCTGGCAAATctct                                                                <  2:407321/143‑1 (MQ=255)                                                                                ggCGCAGCGTGCGTGAACGGCAGCTGACTGAACTGAAAAAATATGCGGTATTCGCTAAAGAGACCATCGCGCCAGACGACCAGCGTGTGCTGATCGGTGATGCTGGATGCAAGGGGCTCGCCGCGCAGGCAAGTCTCTTGAGc                                                           >  2:564026/1‑143 (MQ=255)                                                                                   gcAGCGTGCGTGAACCGCAGCTGACTGAACTGAAAAAATATGCGGTATTCCCTAAAGAGACAATAGCGCCAGACGACGAGCGTGTGCTGCTTGGTGTTGCCGGTGTTGAGGCTCGAGGCGCCCTGCCAAATCTCTTGAGCGaa                                                        >  2:731973/1‑143 (MQ=255)                                                                                     aGCGTGCGTGAACCGCAGCTGACTGAACTGAAAAAATATGCGGTATTCTCTAAAGAGACCATCGCGCCAGACGACGAGCGTGTGCTGCTTGGTGTTGCCGGTTTTCAGGCGCGCGCCGCGCCGGCGAATCGCTTTAGCGACCt                                                      >  1:16166/1‑143 (MQ=255)                                                                                               aaCCGCAGCTGACTGAACTGAAAAAATATGCGGTATTCTCTAAAGAGACCATCGCGCCAGACGACGAGCGTGTGCTGCTTGGTGTTGCCGGTTTTCAGGCGCGCGCCGCGCTGGCAAATCTCTTTAGCGAACTGCCTTCgaaa                                            <  1:640236/143‑1 (MQ=255)                                                                                                         gactgaACGGAAACAAGTTGCGGGATTCTCTCAAGAGACCATCGCGCCAGACGCCGAGCGTGTGCTGCTTGGTGTTGCCGGTTTTCAGGCGCGCGCCGCGCTGGCAAATCTCTTTAGCGAACTGCCTTCGAAAGAAAAACAgg                                  <  2:234122/143‑1 (MQ=255)                                                                                                           ctgaactgaaAAAATATGCGGTATTCTCTAAAGAGACCATCGCGCCAGACGACGAGCGTGTGCTGCTTGGTGTTGCCGGTTTTCAGGCGCGCGCCGCGCTGGCAAATCTCTTTAGCGAACTGCCTGCGAAAGAAAAACCGGTa                                >  2:391519/1‑143 (MQ=255)                                                                                                              aactgaaAAAATATGCGGTATTCTCTAAAGAGACCATCGCGCCAGACGACGAGCGTGTGCTGCTTGGTGTTGCCGGTTTTCAGGCGCGCGCCGCGCTGGCAAATCTCTTTAGCGCACCGCCTTCGAAAGAAAGACAGGTAGTc                             >  2:328066/1‑143 (MQ=255)                                                                                                                ctgaaAAAATATGCGGTATTCTCTAAAGAGACCATCGCGCCAGACGACGAGCGTGTGCTGCTTGGTGTTGCCGGTTTTCAGGCGCGCGCCGCGCTGGCAAATCTCTTTAGCGAACTGCCTTCGAAAGAAAAACAGGTAGTCaa                           <  2:57808/143‑1 (MQ=255)                                                                                                                 tgaaAAAATATGCGGTATTCTCTAAAGAGACCATCGCGCCAGACGACGAGCGTGTGCTGCTTGGTGTTGCCGGTTTTCAGGCGCGCGCCGCGCTGGCAAATCTCTTTAGCGAACTGCCTTCGAAAGAAAAACAGGTAGTCaaa                          <  1:696958/143‑1 (MQ=255)                                                                                                                 tgaaAAAATATGCGGTATTCTCTAAAGAGACCATCGCGCCAGACGACGAGCGTGTGCTGCTTGGTGTTGCCGGCTTTCAGGCGCGCGCCGCGCTGGCAAATCTCTTTAGCGAACTGCCTTCGCAAGAACCACAGGTAGTCaga                          >  2:409536/1‑141 (MQ=255)                                                                                                                    aaaaaTATGCGGTATTCACTAGAGAGACCGTCGCGCCAGACGACGAGCGTGTGCTGCTTGGTGTTGCCGGTTGTCAGGCGCGCGCCGCGCTGGCAAATCTCTTTAGCGAACTGCCTTCGAAAGAAAAACAGGTAGTCAaagaa                       <  2:365955/143‑1 (MQ=255)                                                                                                                     agagtatGCGGTATTCTCTAAAGAGACCATCGCGCCAGACGACGAGCGTGTGCTGCTTGGTGTTGCCGGTTTTCAGGCGCGCGCCGCGCTGGCAAATCTCTTTAGCGAACTGCCTTCGAAAGAAAAACAGGTAGTCAaagaag                      <  2:366787/139‑1 (MQ=255)                                                                                                                         tatGCGGTATTCTCTAAAGAGACCATCGCGCCAGACGACGAGCGTGTGCTGCTTGGTGTTGCCGGTTTTCAGGCGCGCGCCGCGCTGGCAAATCTCTTTAGCGAACTGCCTTCGAAAGAAAAACAGGTAGTCAAAGAAGgcgc                  <  1:18/143‑1 (MQ=255)                                                                                                                           tGCGGTATTCTCTAAAGAGACCATCGCGCCAGACGACGAGCGTGTGCTGCTTGGTGTTGCCGGTTTTCAGGCGCGCGCCGCGCTGGCAAATCTCTTTAGCGAACTGCCTTCGAAAGAAAAACAGGTAGTCAAAGAAGGCGCGa                <  1:785077/143‑1 (MQ=255)                                                                                                                           tGCGGGATGCTCTAAAGAGACCAGCGCGCCAGACGACGAGCGTGTGCTGCTTGGTGTTGCCGGTTTTCAGGCGCGCGCCGCGCTGGCAAATCTCTTTAGCGAACTGCCTTCGAAAGAAAAACAGGTAGTCAAAGAAGGCGCGa                <  1:471371/143‑1 (MQ=255)                                                                                                                              ggTATTCTCTAAAGAGACCATCGCGCCAGACGACGAGCGTGTGCTGCTTGGTGTTGCCGGTTTTCAGGCGCGCGCCGCGCTGGCAAATCTCTTTAGCGAACTGCCTTCGAAAGAAAAACAGGTAGTCAAAGAAGGCGCGACCa             >  1:257826/1‑143 (MQ=255)                                                                                                                                      ctAAAGAGACCATCGCGCCAGACGACGAGCGTGTGCTGCTTGGTGTTGCCGGTTTTCAGGCGCGCGCCGCGCTGGCAAGTCTCTTTAGCGAACTGCCTTCGAAAGAAAAACAGGTAGTCAAAGAAGGCGCGACCACGTTGCTa     >  2:694098/1‑143 (MQ=255)                                                                                                                                       tAAAGAGACCATCGCGCCAGACGACGAGCGTGTGCTGCTTGGTGTTGCCGGTTTTCAGGCGCGCGCCGCGCTGGCAAATCTCTTTAGCGAACTGCCTTCGAAAGAAAAACAGGTAGTCAAAGAAGGCGCGACCACTTTGCTAt    >  1:397551/1‑143 (MQ=255)                                                                                                                                         aaGAGACCATCGCGCCAGACGACGAGCGTGTGCTGCTTGGTGTTTCCGGTTTTCAGGCGCGCGCCGCGCTGACAAATCTCTTTAGCGAACTTCATTCGAAAGAAACACAGGTAGTAAAAGAAGGCGCGACAACATTGCTaaga  >  2:640283/1‑140 (MQ=255)                                                                                                                                         aaGAGACCATCGCGCCAGACGACGAGCGTGTGCTGCTTGGTGTTGCCGGTTTTCAGGCGCGCGCCGCGCTGGCAAATCTCTTTAGCGAACTGCCTTCGAAAGAAAAACAGGTAGTCAAAGAAGGCGCGACCACTTTGCTATgg  >  2:63233/1‑143 (MQ=255)                                                                                                                                            |                                                                                                                                            GCCCATTGCGACGCCAAAGGTAAAATGTGGAGCAATTTACGTCTGTTCCGCGACGGCGATGGCTTTGCATGGATTGAACGGCGCAGCGTGCGTGAACCGCAGCTGACTGAACTGAAAAAATATGCGGTATTCTCTAAAGTGACCATCGCGCCAGACGACGAGCGTGTGCTGCTTGGTGTTGCCGGTTTTCAGGCGCGCGCCGCGCTGGCAAATCTCTTTAGCGAACTGCCTTCGAAAGAAAAACAGGTAGTCAAAGAAGGCGCGACCACTTTGCTATGG  >  NC_000913/3041493‑3041771

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑