Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,352,800 C→A V76F (GTC→TTC)  arcB ← sensory histidine kinase ArcB

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,352,8000CA96.9% 88.9 / ‑3.7 32V76F (GTC→TTC) arcBsensory histidine kinase ArcB
Reads supporting (aligned to +/- strand):  ref base C (1/0);  new base A (11/20);  total (12/20)
Fisher's exact test for biased strand distribution p-value = 3.75e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.83e-01

CGAACGGCAATTTCCTGATTTAGCTGGGCAATATTATCTTTTAACTGAACGTTGAGGCTCAAATCGCGCTCGCGCATCTCCTCCAGTTTTTGCACCAGCCGTGACAGACGTTGTCGTGACTCCTCCAGTTGCTCGACGACCACCGATAGAAAGTAGACCGCCCACGGCGTAATCAGCAAACCAAAGAAGATAGAACGAATAACATCAATGCTTTCGACCTGACCATGCAGCACCATGGTTACCGCCATTTGTACCACAATGGCAAGAACGACGAG  >  NC_000913/3352661‑3352935
                                                                                                                                           |                                                                                                                                       
cGAACGGCAATTTCCTGATTTAGCTGGGCAATATTATCTTTTAACTGAACGTTGAGGCTCAAATCGCGCTCGCGCATCTCCTCCAGTTTTTGCACCAGCCGTGACAGACGTTGTCGTGACTCCTCCAGTTGCTCGACGaacac                                                                                                                                      <  1:76777/143‑1 (MQ=255)
             ccTGATTTAGCTGGGCAATATTATCTTTTAACTGAACGTTGAGGCTCAAATCGCGCTCGCGCATCTCCTCCAGTTTTTGCACCAGCCGTGACAGACGTTGTCGTGACTCCTCCAGTTGCTCGACGAACACCGATCGAAAGTAg                                                                                                                         >  1:237826/1‑143 (MQ=255)
               tGATTTAGCTGGGCAATATTATCTTTTAACTGAACGTTGAGGCTCAAATCGCGCTCGCGCATCTCCTCCAGTTTTTGCACCAGCCGTGACAGACGTTGTCGTGACTCCTCCAGTTGCTCGACGAACAACGATAGAAAGTAGAc                                                                                                                       >  1:507236/1‑143 (MQ=255)
                         gggCACTATTATCTTGTAACGGCACGTGGAGGCTCAAAGCGCGCTCGCGCATCTCCTCCAGTTGTTGCACCAGCCGTGACAGACGTTGTCGTGACTCCTCCAGTTGCTCGACGAACACCGATAGAAAGTAGACCGCCCACGGc                                                                                                             <  1:233088/143‑1 (MQ=255)
                         gggCAATATTATCTTTTAACTGAACGTTGAGGCTCAAATCGCGCTCGCGCATCTCCTCCAGTTTTTGCACCAGCCGTGACAGACGTTGTCGTGACTCCTCCAGTTGCTCGACGAACACAGATAGAAAGTAGACCGCCCACGGc                                                                                                             >  1:767058/1‑143 (MQ=255)
                              acattatCTTTTAACTGAACGTTGAGGCTCAAATCGCGCTCGCGCATCTCCTCCAGTTTTTGCACCAGCCGTGACAGACGTTGTCGTGACTCCTCCAGTTGCTCGACGAACACCGATAGAAAGTAGACCGCCCACGGCGTAAt                                                                                                        <  2:151909/141‑1 (MQ=255)
                                     cTTTTAACTGAACGTTGAGGCTCAAATCGTGCTCGCGCATCTCCTCCAGTTTTTGCACCAGCCGTGACCGACAATGCCGTGAATCCTACAGTTGCTCGACGAACACCGATAGAAAGTAGACCGCCCACGGCGTAATCAGCaaa                                                                                                 <  1:459038/143‑1 (MQ=255)
                                          aaCTGAACGTTGAGGCTCAAATCGCGCTCGCGCATCTCCTCCAGTTTTTGCACCAGCCGTGACAGACGTTGTCGTGACTCCTCCAGTTGCTCGACGAACACAGATAGAAAGTAGACCGCCCACGGCGTAATCAGCAAACCaaa                                                                                            <  2:767058/143‑1 (MQ=255)
                                            cTGAACGTTGAGGCTCAAATCGCGCTCGCGCATCTCCTCCAGTTTTTGCACCAGCCGTGACAGACGTTGTCGTGACTCCTCCAGTTGCTCGACGACCACCGATAGAAAGTAGACCGCCCACGGCGTAATCAGCAAACCAaaga                                                                                          >  1:628055/1‑143 (MQ=255)
                                                  gTTGAGGCTCAAATCGCGCTCGCGCATCTCCTCCAGTTTTTGCACCAGCCGTGACAGACGTTGTCGTGACTCCTCCAGTTGCTCGACGAACACCGATAGAAAGTAGACCGCCCACGGCGTAATCAGCAAACCAAAGAAGATAg                                                                                    >  1:318128/1‑143 (MQ=255)
                                                                 gtgcgcgcgcCGCTCCTCCAGTTTTTGCACCAGCCGTGACAGACGGTGTCGTGACTCCTCCAGTTGCTCGACGAACACCGATAGAAAGTAGACCGCCCACGGCGTAATCAGCAAACCAAAGAAGATAGAACGAATAACATCaa                                                                     <  2:273164/138‑1 (MQ=255)
                                                                      cgcgcATCTCCTCCAGTTTTTGCACCAGCCGTGACAGACGTTGTCGTGACTCCTCCAGTGGCTCGACGAACACCGATAGAAAGTAGACCGCCCACGGCGTAATCAGCAAACCAAAGAAGATAGAACGAATAACATCAATGCtt                                                                <  1:506178/143‑1 (MQ=255)
                                                                       gcgcATCTCCTCCAGTTTTTGCACCAGCCGTGACAGACGTTGTCGTGACTCCTCCAGTTGCTCGACGAACACCGCTAGAAAGTAGACCGCCCACGGCGTAATCAGCAAACCAAAGAAGATAGAACGAATAACATCAATGCttt                                                               >  1:456357/1‑143 (MQ=255)
                                                                        cgcATCTCCTCCATTTGTGGTACCAGCCGGTACATACGTTGTCTCTGCTCCTCCAGTTGCTCGACGAACACCGATAGCAAGTAGACCGCCCACTGCGTAATCAGCAAACCCAAGAAGATAGAACGAATAACATCAATGCTTTc                                                              <  2:383086/143‑1 (MQ=255)
                                                                          cATCTCCTCCAGTTTTTGCACCAGCCGTGACAGACGTTGTCGTGACTCCTCCAGTTGCTCGACGAACACCGATAGAAAGTAGACCGCCCACGGCGTAATCAGCAAACCAAAGAAGATAGAACGAATAACATCAATGCTTTCGa                                                            >  1:482250/1‑143 (MQ=255)
                                                                           aTCTCCTCCAGTTTTTGCACCAGCCGTGACAGACGTTGGCGTGACTCCTCCAGTTGCTCGACGAACACCGATAGAAAGTAGACCGCCCACGGCGTAATCAGCAAACCAAAGAAGATAGAACGAATAACATCAATGCTTTCGAc                                                           <  2:237826/143‑1 (MQ=255)
                                                                               cctcgcttttgtGCACCCCTCGGGACATACTTTGTCGTGACTCCTCCAGTTGCTCGACGAACACCGATATATAGTAGACCGCCCACTGCGTAATCAGCAAACCAAAGAAGATAGAACGAATAACATCAATGCTTTCGACCTGa                                                       <  2:405503/136‑1 (MQ=255)
                                                                               cctccAGTGTTCCAACCCGCCGTGACAGACGTTGTCGTGACTCCTCCAGTTGCTCGACGAACACCGATAGAAAGTAGACCGCCCACGGCGTAATCAGCAAACCAAAGAAGATAGAACGAATAACATCAATGCTTTCGACCTGa                                                       <  2:234303/143‑1 (MQ=255)
                                                                                 tccAGTTTTTGCACCAGCCGTGACAGACGTTGTCGTGACTCCTCCAGTTGCTCGACGAACACCGATAGAAAGTAGACCGCCCACGGCGTAATCAGCAAACCAAAGAAGATAGAACGAATAACATCAATGCTTTCGACCTGAcc                                                     >  1:156472/1‑143 (MQ=255)
                                                                                     gTTTTCGCACCAGCCTGGACAGACGTTCTGCGGGCTCCTCCAGTTGCTCGACGAACACCGATAGAAAGTAGACCGCCCACGGCGTAATCAGCAAACCAAAGAAGATAGAACGAATAACATCAATGCTTTCGACCTGACCATgc                                                 <  2:384806/143‑1 (MQ=255)
                                                                                        tttGCACCAGCCGTGACAGACGTTGTCGTGACTCCTCCAGTTGCTCGACGAACACCGATAGAAAGTAGACCGCCCACGGCGTAATCAGCAAACCAAAGAAGATAGAACGAATAACATCAATGCTTTCGACCTGACCATgcagc                                              >  1:124124/1‑143 (MQ=255)
                                                                                           gCACCAGCCGTGACAGACGTTGTCGTGACTCCTCCAGTTGCTCGACGAACACCGATAGAAAGTAGACCGCCCACGGCGTAATCAGCAAACCAAAGAAGATAGAACGAATAACATCAATGCTTTCGACCTGACCATGCAGCAcc                                           <  2:318128/143‑1 (MQ=255)
                                                                                             aCCAGCCGTGACAGACGTTGTCGTGACTCCTCCAGTTGCTCGACAAACACCTAGAGAAAGTAGCCCGCCCACGGCGTAATCAGCACACCAAAGAAGATAGAACAACTAACATCAATGCTTCCGACCGCAAAATGCAGCACCAt                                         >  2:569949/1‑143 (MQ=255)
                                                                                              ccAGCCGTGACAGACGTTGTCGTGACGCCTCCAGTTGCTCGACGAACACCGATAGAAAGTAGACCGCCCACGGCGTAATCAGCAAACCAAAGAAGATAGAACGAATAACATCAATGCTTTCGACCTGACCATGCAGCACCATg                                        <  1:127972/143‑1 (MQ=255)
                                                                                                     tcaaaGACGTTGTCGTGTCGCCTCCAGTTGCTCGACGAACACCGATGGAAAGTAGACCGCCCACGGCGTAATCAGCAAACCAAAGAAGATAGAACGAATAACATCAATGCTTTCGACCTGACCATGCAGCACCATGGTTACCg                                 <  2:46564/139‑1 (MQ=255)
                                                                                                      gACAGACGTTGTCGTGACTCCTCCAGTTGCTCGACGAACACCGATAGAAAGTAGACCGCCCACGGCGTAATCAGCAAACCAAAGAAGATAGAACGAATAACGTCAGACAGTGCTTCCGCACCATGCAGCACTATGGTTACCGc                                <  2:173755/143‑1 (MQ=255)
                                                                                                         aGACGTTGTCGTGACTCCTCCAGTTGCTCGACGAACACCGATAGAAAGTAGACCGCCCACGGCGTAATCAGCAAACCAAAGAAGATAGAACGAATAACATCAATGCTTTCGACCTGACCATGCAGCACCATGGTTACCGCCAt                             >  1:354161/1‑143 (MQ=255)
                                                                                                           aCGTTGTCGTTACTCCTCCAGTTGCTCGACGAACACCGATAGAAAGTAGACCGCCCACGGCGTAATCAGCAAACCAAAGAAGATAGAACGAATAACATCAATGCTTTCGACCTGACCATGCAGCACCATGGTTACCGCCAttt                           <  2:548460/143‑1 (MQ=255)
                                                                                                             gTTGTCGTGACTCCTCCAGGTGCTCGACGAACACCGATAGAAAGTAGACCGCCCACGGCGTAATCAGCAAACCGAAGAAGATAGAACGAATAACATCAATGCTTTCGATCTGACCATGCAGCACCATGGTTACCGCCATTTGt                         <  2:335101/143‑1 (MQ=255)
                                                                                                              ctGTCGGGACTCCTCCAGTTGCTCGACGAACACCGATAGAAAGTAGACCGCCCACGGCGTAATCAGCAAACCAAAGAAGATAGAACGAATAACATCAATGCTTTCGACCTGACCATGCAGCACCATGGTTACCGCCATTTGTa                        <  2:427683/142‑1 (MQ=255)
                                                                                                                  cGTGCCTCTTCCAGTTGCTCGACGAACACCGATAGAAAGTAGACCGCCCACGGCGTAATCAGCAAACCAAAGAAGATAGAACGAATAACATCAATGCTTTCGACCTGACCATGCAGCACCATGGTTACCGCCATTTGTACcac                    <  2:305157/143‑1 (MQ=255)
                                                                                                                        tcctccAGTTGCTCGACGAACACCGATAGAAAGTAGACCGCCCACGGCGTAATCAGCAAACCAAAGAAGATAGAACGAATAACATCAATGCTTTCGACCTGACCATGCAGCACCATGGTTACCGCCATTTGTACCACAATGGc              >  1:302858/1‑143 (MQ=255)
                                                                                                                                    tCGACGAACACCGATAGAAAGTAGACCGCCCACGGCGTAATCAGCAAACCAAAGAAGATAGAACGAATAACATCAATGCTTTCGACCTGACCATGCAGCACCATGGTTACCGCCATTTGTACCACAATGGCAAGAACGACGAg  <  1:481068/143‑1 (MQ=255)
                                                                                                                                           |                                                                                                                                       
CGAACGGCAATTTCCTGATTTAGCTGGGCAATATTATCTTTTAACTGAACGTTGAGGCTCAAATCGCGCTCGCGCATCTCCTCCAGTTTTTGCACCAGCCGTGACAGACGTTGTCGTGACTCCTCCAGTTGCTCGACGACCACCGATAGAAAGTAGACCGCCCACGGCGTAATCAGCAAACCAAAGAAGATAGAACGAATAACATCAATGCTTTCGACCTGACCATGCAGCACCATGGTTACCGCCATTTGTACCACAATGGCAAGAACGACGAG  >  NC_000913/3352661‑3352935

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑