| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 4,123,110 | G→A | P77L (CCA→CTA) | ftsN ← | cell division protein FtsN |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 4,123,110 | 0 | G | A | 95.0% | 95.2 / NA | 40 | P77L (CCA→CTA) | ftsN | cell division protein FtsN |
| Reads supporting (aligned to +/- strand): ref base G (0/0); major base A (15/23); minor base C (2/0); total (17/23) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.74e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.97e-01 | |||||||||||
GTTCAAGAAGCTGACGTTGTTCTGGTGTCAGTTGCTCCGGCGTTTTCACTTCACCACCGGCAGAAGGTTCTGTGGGCGCACGCACTCCCGGCTGGCGACTTTCCAGCTCTTTAATGTAGCGCCAGCGTTCTTCTGGTTTTGGTGGTAGTCCGTTTCCGGTCACTTTCTGGCTTTGCAGCGTCTCGGACTCTTCTTTCTTGTGATGCGTAATGAAGTACAGACCACCGATAAAGGTCACAAGAACGGCGGCAGCAATAGCGACCATAGCGGGAGAAACCGCA > NC_000913/4122970‑4123250 | gTTCAAGAAGCTCACGTTGTTCTGGTGTCAGTTGCTCCGGCGTTTTCACTTCACCACCGGCAGAAGGTTCTGTGGACGCACGCACTCCCGGCTGGCGACTTTCCAGCTCTTTAATGTAGCGCCAGCGTTCTTCTGGTTTtagt < 1:570371/143‑1 (MQ=255) tCAAGGCGCTGACGTTGTTCTGGTGTCAGTTGCTCCGGCGTTTTCACTTCACCACCGGCAGAAGGTTCTGTGGGCGCACGCACTCCCGGCTGGCGACTTTCCAGCTCTTTAATGTAGCGCCAGCGTTCTTCTGGTTTtagtgg < 2:799182/143‑1 (MQ=255) aagaagCTGACGTTGTTCTGGTGTCAGTTGCTCCGGCGTTTTCACTTCACCACCGGCAGAAGGTTCTGTGGGCGCACGCACTCCCGGCTGGCGACTTTCCAGCTCTTTAATGTAGCGCCAGCGTTCTTCTGGTTTTAGTGGTa < 1:218582/143‑1 (MQ=255) ttgttCTGGTGTCAGTTGCTCCGGCGTTTTCACTTCACCACCGGCAGAAGGTTCTGTGGGCGCACGCACTCCCGGCTGGCGACTTTCCAGCTCTTTCCTGTAGCGCCCGCGTTCTTCTGGTTTTAGGGATAGTCCGTTGccag > 1:283035/1‑141 (MQ=255) tGGTGTCAGTTGCTCCGGCGTTTTCACTTCACCACCGGCAGAAGGTTCTGTGGGCGCACGCACTCCCGGCTGGCGACTCTCCAGCTCTTTAATGTAGCGCCAGCCTTCTTCTGGTCTTCGTGGTAATCCATTTCCgctaacgt > 1:256058/1‑136 (MQ=255) gtgtCAGTTGCTCCGGCGTTTTCACTTCACCACCGGCAGAAGGTTCTGTGGGCGCACGCACTCCCGGCTGGCGACTTTCCAGCTCTTTAATGTAGCGCCAGCGTTCTTCTGGTTTTAGTGGTAGTCCGTTTCCGGTCACTTTc > 1:595298/1‑143 (MQ=255) tCAGTTGCTCCGGCGTTTTCACTTCACCACCGGCAGAAGGTTCTGTGGGCGCACGCACTCCCGGCTGGCGACTTTCCAGCTCTTTAATGTAGCGCCAGCGTTCTTCTGGTTTTAGTGGTAGTCCGTTTCCGGTCACTTTCTgg < 1:694870/143‑1 (MQ=255) aGTTGCTCCGGCGTTTTCACTTCACCACCGGCAGAAGGTTCTGTGGGCGCACGCACTCCCGGCTGGCGACTTTCCAGCTCTTTCATGTCGCGCCAGCGTTCTTCTGGTTTTAGTGGTAGTCCGTTTCCAGTCACTTTATTGCt > 1:325018/1‑143 (MQ=255) gTTGCTCCGGCGTTTTCACTTCACCACCGGCAGAAGGTTCTGTGGGCGCACGCACTCCCGGCTGGCGCCTTTCCAGCTCTTTAATGTAGCGCCAGCGTTCTTCTGGTTTTAGTGGTCGTCCGTTTCCGGTCACTTTCTGGCtt > 1:272179/1‑143 (MQ=255) gCTCCGGCGTTTTCACTTCACCACCGGCAGAAGGTTCTGTGGGCGCACGCACTCCCGGCTGGCGACTTTCCAGCTCTTTAATGTAGCGCCAGCGTTCTTCTGGTTTTAGTGGTAGTCCGTTTCCGGTCACTTTCTGGCTTTGc < 1:187911/143‑1 (MQ=255) tCCGGCGTTTTCACTTCACCACCGGCAGAAGGTTCTGTGGGCGCACGCACTCCCGGCTGGCGACTTTCCAGCTCTTTAATGTAGCGCCAGCGTTCTTCTGGTTTTAGTGGTAGTCCGTTTCCGGTCACTTTCTGGCTTTGCAg > 1:655820/1‑143 (MQ=255) ccgccgCTTTCGCTTCACCTCCGGCAGAAGGTTCTTTGGGCTCACGCACTCCCGGCTGGCGACTTTCCAGCTCTTTCATTTAGCGCCAGCGTTCTTCTGGTTTTAGTGGTAGTCCGTTTCCGGTAACTGTGTGGCTTTGCAGc < 2:59673/143‑1 (MQ=255) cGGCGTTTTCACTTCACCACCGGCAGAAGGTTCTGTGGGCGCACGCACTCCCGGCTGGCGACTTTCCAGCTCTTTAATGTAGCGCCCGCGTGCTTCTGGTGTTCGTGGTGGTCGGTCTCCGGTCGCGTTCTGGCTTTgtcgcg > 2:705110/1‑138 (MQ=255) cGGCGTTTTCACTTCACCACCGGCAGAAGGTTCTGTGGGCGCACGCACTCCCGGCTGGCGACTTTCCAGCTCTTTAATGTAGCGCCAGCGTTCTTCTGGTTTTACTGGTCGTCCGTTTCCGGTCACTTTCTGCCTTTGccccg > 2:337136/1‑139 (MQ=255) gTTTTCACTTCACCACCGGCAGAAGGTGCTGTGGGCGCACGCACTCCCGGCTGGCGACTTTCCAGCTCTGTAATGTAGCGCCAGCGTTCTTCTGGTTTTAGTGGTAGTCCGTTTCCGGTCACTTTCTGGCTTTGCAGCGtctc < 1:337136/143‑1 (MQ=255) gTTTTCACTTCACCACCGGCAGAAGGCTCTGTGGGCGCACGCACTCCCGGCTGGCGACTTTCCAGCTCTTTAATGTAGCGCCAGCGTTCTTCTGGTTTTAGTGGTAGTCCGTTTCCGGTCACTTTCTGGCTTTGCAGCGtctc < 1:465305/143‑1 (MQ=255) ttttCACTTCACCCCCGGCAGAAGGTTCTGTGGGCGCACGCACTCCCGGCTGGCGACTTTCCAGCTCTTTAATGTGGCGCCAGCGTTCTTCTGGTTTTAGTGGTAGTCCGTTTCCGGTCACTTTCTGGCTTTGCAGCGTCTCg < 1:98540/143‑1 (MQ=255) tttCACTTCACCACCGGCAGAAGGTTCTGTGGGCGCACGCACTCCCGGCTGGCGACTTTCCAGCTCTTTAATGTAGCGCCAGCGTTCTTCTGGTTTTAGTGGTAGTCCGTTTcc < 2:291670/114‑1 (MQ=255) tttCACTTCACCACCGGCAGAAGGTTCTGTGGGCGCACGCACTCCCGGCTGGCGACTTTCCAGCTCTTTAATGTAGCGCCAGCGTTCTTCTGGTTTTAGTGGTAGTCCGTTTcc > 1:291670/1‑114 (MQ=255) tttCACTTCACCACCGGCAGAAGGTTCTGTGGGCGCACGCACTCCCGGCTGGCGACTTTCCAGCTCTTTAATGTAGCGCCAGCGTTCTTCTGGTTTTAGTGGTAGTCCGTTTCCGGTCACTTTCTGGCTTTGCAGCGTCTCgg > 1:742/1‑143 (MQ=255) cGGCAGAAGGTTCTGTGGGCGCTCGCACTCCCGGCTGGCGACTTTCCAGCTCTTTAATGTAGCGCCAGCGTTCTTCTGGTTTTAGTGGTAGTCCGTTTCCGGTCACTTTCTGGCTTTGCAGCGTCTCGGACTCTTCTTTCTtg < 1:309572/143‑1 (MQ=255) tCTGTGGGCGCACGCACTCCCGGCTGGCGACTTTCCAGCTCTTTAATGTAGCGCCAGCGTTCTTCTGGTTTTAGTGGTAGTCCGTTTCCGGTCACTTTCTGGCTTTGCAGCGTCTCGGACTCTTCTTTCTTGTGATGCGTAAt < 1:21592/143‑1 (MQ=255) cTGTGGGCGCACGCACTCCCGGCTGGCGACTTTCCAGCTCTTTAATGTAGCGCCAGCGTTCTTCTGGTTTTAGTGGTAGTCCGTTTCCGGTCACTTTCTGGCTTTGCAGCGTCTCGGACTCTTCTTTCTTATGATGCGTAATg > 1:748243/1‑143 (MQ=255) ggCGCACGCACTCCCGGCTGGCGACTTTCCAGCTCTTTAATGTAGCGCCAGCGTTCTTCTGGTTTTAGTGGTAGTCCGTTTCCGGTCACTTTCTGGCTTTGCAGCGTCTCGGACTCTTCTTTCTTGTGATGCGTAATGAAGTa < 1:604913/143‑1 (MQ=255) ggCGCACGCACTCCCGGCTGGCGACTTGCCAGGTCGTTAACGTAGCGCCAGCGTTCTTCTGGCCGTAGTGGTAGTCCGTTTCGCGTCACTTTTTGGCTTTGGAGCGGCTCGGCCTCTTCTCTCCTGTGATGCGTCATGACGtg > 2:115513/1‑142 (MQ=255) gcgcACGCGCTCCCGGCTGGCGACTTGCCAGCTCTTTAATGTAGCGCCAGCGTTCTTCTGGTTTTAGTGGTAGTCCGTTTCCGGTCACTTTCTGGCTTTGCAGCGTCTCGGACTCTTCTTTCTTGTGATGCGTAATGAAGTAc < 2:628556/143‑1 (MQ=255) tCCCGGCTGGCGACTTTCGAGCCCTTTGATGTAGCGCCAGCGTTCTTCTGGTTTTAGTGGTAGTCCGTTTCCGGTCACTTTCTGGCTTTGCAGCGTCTCGGACTCTTCTTTCTTGTGATGCGTAATGAAGTACAGACCACCGa < 2:237496/143‑1 (MQ=255) tCCCGGCTGGCGACTTTCCAGCTCTTTAATGTAGCGCCAGCGTTCGTCTGGTTTTAGTGGTAGTCCGTTTCCGGTCACTTTCTGGCTTTGCAGCGTCTCGGACTCTTCTTTCTTGTGATGCGTAATGAAGTACAGACCACCGa > 2:169966/1‑143 (MQ=255) cGGCTGGCGATTTTCCAGCTCTTTAATGTAGCGCCATCGTTCTTCTGGTTTTAGTGGTAGTCCGTTTCCGGTCACTTTCTGGCTTTGCAGCGTCTCGGACTCTTCTTTCTTGTGATGCGTAATGAAGTACAGACCACCGATaa < 2:645031/143‑1 (MQ=255) ggCGCCCTTGCAGCTCGTTAATGTAGCGCCCGCGTTCTTCTGGTTTTAGTGGTACTCCGTTTCCTGTCACTTTCTGGCTTTGCAGCGTCTCGGACTCTTCTTTCTTGTTATGCTTAATGAAGTACAGACCACCGATAAAGGTc < 1:455360/143‑1 (MQ=255) gCGTCTTTCCAGCTCTTTAATGTAGCGCCAGCGTTCTTCTGGTTTTAGTGCTAGTCCGTGCCTGGTCACTTTCTGGCTTTGCAGCGTCTCGGACTCTTCTTTCTTGTGATGCGTAATGAAGTACAGACCACCGATAAAGGTca < 2:693482/143‑1 (MQ=255) gACTTTCCAGCTCTTTAATGTAGCGCCAGCGTTCTTCTGGTTTTAGTGGTAGTCCGTTTCCGGTCACTTTCTGGCTTTGCAGCGTCTCGGACTCTTCTTTCTTGTGATTCGCAACTAAACACAGACCACCGATAAAGGTcaca < 2:655820/143‑1 (MQ=255) tttCCAGCTCTTTAATGTAGCGCCAGCGTTCTTCTGGTTTTAGTGGTAGTCCGTTTCCGGTCACTTTCTGGCTTTGCAGCGTCTCGGACTCTTCTTTCTTGTGTTGCGTGATGAAGTACAGACCACCGATCACGGGCACAAGa > 2:53515/1‑143 (MQ=255) gCTCTTTAATGTAGCGCCAGCGTTCTTCTGGTTTTAGTGGTAGTCCGTTTCCGGTCACTTTCTGGCTTTGCAGCGTCTCGGACTCTTCTTTCTTGTGATGCGTAATGAAGTACAGACCACCGATACAGGTCACAAGAAcggcg > 1:86585/1‑143 (MQ=255) ctctTTAATGTGGCGCCAGCGTTCTTCTGGTTTTAGGGGTAGTCCGTTTCCGGTCACTTTCTGGCTTTGCAGCGTCTCGGACTCTTCTTTCTTGTGATGCGTAATGAAGTACAGACCACCGATAAAGGTCACAAGAAcggcgg < 2:371178/143‑1 (MQ=255) ctctTTAATGTAGCGCCAGCGTTCTTCTGGTTTTAGTGGTAGTCCGTTTCCGGTCACTTTCTGGCTTTGCAGCGTCTCGGACTCTTCTTTCTTGTGATGCGTAATGAGGTACAGCCCACCGATAAAGGTCACAAGAAcggcgg > 1:62773/1‑143 (MQ=255) tttAATGTATCGCCAGCGTTCTTCTTGTTTTAGTGGTTGTCCGTTTCCGGTCACTTTCTGGCTTTGCAGCGTCTCGGACTCTTCTTTCTTGTGATGCGTAATGAAGTACAGACCACCGATAAAGGTCACAAGAACGGCGgcag < 1:123617/143‑1 (MQ=255) cAGCGTTCTTCTGGTTTTAGTGGTAGTCCGGTTCCGGTCACTTTCTGGCTTTGCAGCGTCTCGGACTCTTCTTTCTTGTGATGCGTAATGAAGTACAGACCACCGATAAAGGTCACAAGAACGGCGGCAGCAATAGCGACCAt < 2:595298/143‑1 (MQ=255) ttcttcTGGTTTTAGTGGTAGTCCGTTTCCGGTCACTTTCTGGCTTTGCAGCGTCTCGGACTCTTCTTTCTTGTGATGCGTAATGAAGTACAGACCACCGATAAAGGTCACAAGAACGGCGGCAGCAATAGCGACCATAGCgg < 1:169966/143‑1 (MQ=255) cttccGGTTGTAGTGGTAGTCCGTGTCCGGGCACTTTCTGGCTTTGCAGCGTCTCGGACTCTTCTTTCTTGTGATGCGTAATGAAGTACAGACCACCGATAAAGGTCACAAGAACGGCGGCAGCAATAGCGACCATAGCGGga < 1:311096/143‑1 (MQ=255) ctGGTTTTAGTGGTAGTCCGTTTCCGGTCACTTTCTGGCTTTGCAGCGTCTCGGACTCTTCTTTCTTGTGATGCGTAATGAAGTCCAGACCACCGATAAAGGTCACACGAACGGCGGCAGCAATAGCGCCCATAGCTGgagca > 2:557950/1‑141 (MQ=255) tgtttttAGTGGTAGTCCGTTTCGCGTCACTTTCTGGCTTTGCAGCGTCTCTGACTCTTGTTTCTTGTGCTTCTTAATGAACTACAGACCACCGCTAAAGGTCACAAGAACGGCGGCAGCAATAGCGACCATAGCGGGAGaaa < 2:283035/140‑1 (MQ=255) tgggtggtAGTGCGTTGGCGGTGCTTTTGTGGCTTTGCAGCGTCTGGGACTCTGCCTGCTTGTGATGCGTAAGGAAGTACAGACCACCGATAAAGGTCACAAGAACGGCGGCAGCAATAGCGACCATAGCGGGAGAAACCgca < 2:383959/141‑1 (MQ=255) | GTTCAAGAAGCTGACGTTGTTCTGGTGTCAGTTGCTCCGGCGTTTTCACTTCACCACCGGCAGAAGGTTCTGTGGGCGCACGCACTCCCGGCTGGCGACTTTCCAGCTCTTTAATGTAGCGCCAGCGTTCTTCTGGTTTTGGTGGTAGTCCGTTTCCGGTCACTTTCTGGCTTTGCAGCGTCTCGGACTCTTCTTTCTTGTGATGCGTAATGAAGTACAGACCACCGATAAAGGTCACAAGAACGGCGGCAGCAATAGCGACCATAGCGGGAGAAACCGCA > NC_000913/4122970‑4123250 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |