Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	696,470	C→T	noncoding (35/75 nt)	glnX ←	tRNA‑Gln

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	696,470	0	C	T	100.0%	67.0 / NA	27	noncoding (35/75 nt)	glnX	tRNA‑Gln
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (14/13);  total (14/13)

GTCGCATCCGACATTGAAGGATAAGACGTGTCAACATCGCATTCGACATTGAATGAACGCAGAAAAGCAAAAAGCTCGCCGAAGCGAGCTTTTTTAATGTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACTGGGTGCACTTACAAGGTAAGCGTCTTGAATAAATTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCAGAATCCGGTGCCTTACCGCTTGGCG  >  NC_000913/696331‑696608                                                                                                                                            |                                                                                                                                           ggcGCATCCGACATTGCAGGTGAAGACGTGTCAACATCGCATTGGACATTGAATGCACGCAGAAAAGCAACAAGCTCGCCGAAGCGAGCTTTTTTAATGTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATTAGa                                                                                                                                         <  2:657156/141‑1 (MQ=38)    gCATCCGACATTGAAGGATAAGACGTGTCAACATCGCATTCGACATTGAATGAACGCAGAAAAGCAAAAAGCTCGCCGAAGCGCGCTTTTTTACTGTGGCTGGGGTACGAGGATACGAACATCGAAATGACGGAATTAGAATc                                                                                                                                      >  1:8027/1‑143 (MQ=255)       tCCGACATTGAAGGATAAGACGTGTCAACATCGCATTCGACATTGAATGAACGCAGAAAAGCCAAAAGCTCGCCGAAGCGAGCTTTTTTAATGTGGCTGCGGTACGAGGATTCGAACCCCGGAATGCCGGAATTAGAATCCgg                                                                                                                                   >  2:538027/1‑143 (MQ=255)        ccGACATTGAAGGATAAGACTTGTCATCATCGCATTCGACATTGAATGAACGCAGAAAAGCAAAAAGCTCGCCGAAGCGAGCTTTTTTAATGTGGCTGGTGTACGAGGATTCGAACCTCGGAATGCCGGAATTAGAATCCGGt                                                                                                                                  <  2:5355/143‑1 (MQ=255)         cGACATTGAAGGATAAGACGTGTCAACATCGCATTCGACATTGAATGAACGCAGAAAAGCAAAAAGCGCGCCGAAGCGAGCTTTTTTAATGTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATTAGAATCCGGTg                                                                                                                                 <  1:522446/143‑1 (MQ=255)          gACATTGAAGGATAAGACGTGTCAACATCGCATTCGACAGTGAATGAACGCAGAAAAGCAAAAAGCTCGCCGAAGCGAGCTTTTTTAATGTGGCTGGGGTACGAGGATTCGAACCTCGGCCTGCCGGAATTAGAATCCGGTGc                                                                                                                                >  1:223188/1‑143 (MQ=255)             aTTGAAGGATAAGACGTGTCAACATCGCATTCGACATTGAATGAACGCAGAAAAGCAAAAAGCTCGCCGAAGCGAGCTTTTTTAATGTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATTAGAATCCGGTGCCtt                                                                                                                             >  1:168574/1‑143 (MQ=255)                          aCGTGTCAACATCGCATTCGACATTGAATGAACGCAGAAAAGCAAAAAGCTCGCCGAAGCGAGCTTTTTTAATGTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATTAGAATCCGGTGCCTTACCGCTTGGCGAt                                                                                                                >  2:619789/1‑143 (MQ=255)                                     tCGCATTCGACATTGAATGAACGCAGAAAAGCAAAAAGCTCGCCGAAGCGAGCTTTTTTAATGTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATTAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACTgg                                                                                                     >  2:798412/1‑143 (MQ=255)                                      cGCATTCGACATTGAATGAACGCAGAAAAGCAAAAAGCTCGCCGAAGCGAGCTTTTTTAATGTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATTAGAATCCGGTGCCTTACCGGCTGGCGATGCACCACCTggg                                                                                                    >  2:466020/1‑143 (MQ=25)                                        cATTCTACATTGAAGTAACGAAGAAAAGCAAAAATCCCGCCGAAGCGAGCTTTTTGAAGGAGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATTAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACTGGGTg                                                                                                  <  2:281134/143‑1 (MQ=18)                                              aCATTGAATGAACGCAGAAAAGCAAAAAGCTCGCCGAAGCGAGCTTTTTTAATGTGGCTGGGGTACGAGGCTTCGAACCTCGGAATGCCGGAATTAGAATCCGGTGCCTTGCCGCTTGGCGGTACCCCAACTGGGTGCGCTTa                                                                                            >  1:235949/1‑143 (MQ=25)                                                aTTGAATGAACGCAGAAAAGCAAAAAGCTCGCTGAAGCGAGCTTTTTTAATGTGGCTGGGGTACGAGGATTCGAACCGGGGAATGCCGGAATTAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACTGGGTGCACTTACa                                                                                          <  1:114092/143‑1 (MQ=255)                                                aTTGAATGAACGCAGAAAAGCAAAAAGCTCGCCGAAGCGAGCTTTTTTAATGTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATTAGAATCCGGTGCCTTACCGCTTGGCGCTACCCCAACTAGGTGCACTTACa                                                                                          >  2:556262/1‑143 (MQ=255)                                                                           gtCGCCGAAGTGAGCTTTGTTAATGTGGCTGTGGTATGAGGATTCGAACCTCGGAATGCGGGAAGTAGAATCCGGTGCCTTACCGCTTGGCGATAACCCAACTGGGTGCACTTACAAGGTAAGCGTCTTGAATAAATTGGCTg                                                               <  2:517981/142‑1 (MQ=25)                                                                                         cTTTTTTAATGTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATTAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACTGGGTGCACTTACAAGGTAAGCGTCTTGAATAAATTGGCTGGGGTACGAGGATTc                                                 >  1:651241/1‑143 (MQ=255)                                                                                            ttttAATGGGGCTGGGGTACGAGGATTGGGAGCTCGGAATGACGGAATTAGAGTGGGGTGCGTTAGGGTTTGGGGATAACGCAACTGGGTATACGTACGTGGTGGGGGTGTTGAATAAATTGGCTGGGGTACGAGGATTCGaa                                              <  2:530191/143‑1 (MQ=255)                                                                                              ttAATGTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATTAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACTGGGTTCACTTGCAAGGTAAGCGTCTTGAATAAATTGGCTGGGGTACGAGGGTTCGAAcc                                            >  1:128531/1‑143 (MQ=255)                                                                                                aaTGTGGCTGGGGTAGGAGGATTCGAACCTCGCAATGCGGGAATTAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACTGGGTGCACTTACAAGGTAAGCGTCTTGAATAAATTGGCTGGGGTACGAGGATTCGAACCTc                                          <  2:48495/143‑1 (MQ=255)                                                                                                        tGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATTAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACTGGGTGCACTTACAAGGTAAGCGTCTTGAATAAATTGGCTGGGGTACGAGGATTCGAACCTCGGAATGcc                                  <  1:417307/143‑1 (MQ=255)                                                                                                         gtggTACGAGGATTCGAAGCTCGGAATGCCGGAATTAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACTGGGTGCACTTACAAGGTAAGCGTCTTGAATAAATTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCg                                 <  1:466020/141‑1 (MQ=255)                                                                                                                 aGGATTCGAACCTCGGAATGCCGGAATTAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACTGGGTGCACTTACAAGGTAAGCGTCTTGAATAAATTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCAGa                         <  1:278187/143‑1 (MQ=255)                                                                                                                        gAACCTCGGAATGCCGGAATTAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACTGGGTGCACTTACAAGGTAAGCGTCTTGAATAAATTGGCTGGGGTACGAGGCTTCGAACCTCGGAATGCCGGAATCACAATCCGGt                  >  1:261657/1‑143 (MQ=255)                                                                                                                        caaCCTCGGAACGCCGGAATTAGAATCCGGTGCCTTCCCGCTTAGCGATACCCCAACTGGGTGCACTTACAATCTAAGCGTCTTGAATAAATTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCAGAATCCGGt                  <  1:330750/142‑1 (MQ=37)                                                                                                                                     ccGGAATTAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACTGGGTGCACTTACAAGGTAAGCGTCTTGAATAAATTGGCTGGGGTACGAGGATTCg                                                >  2:36586/1‑100 (MQ=255)                                                                                                                                     ccGGAATTAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACTGGGTGCACTTACAAGGTAAGCGTCTTGAATAAATTGGCTGGGGTACGAGGATTCg                                                <  1:36586/100‑1 (MQ=255)                                                                                                                                        gAATTAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACTGGGTGCACTTACAAGGTAAGCGGCTTGAATAAATTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAGTCAGAATCCGGTGCCGTACCGCTTGGCg  >  1:756010/1‑143 (MQ=255)                                                                                                                                            |                                                                                                                                           GTCGCATCCGACATTGAAGGATAAGACGTGTCAACATCGCATTCGACATTGAATGAACGCAGAAAAGCAAAAAGCTCGCCGAAGCGAGCTTTTTTAATGTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACTGGGTGCACTTACAAGGTAAGCGTCTTGAATAAATTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCAGAATCCGGTGCCTTACCGCTTGGCG  >  NC_000913/696331‑696608

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑