| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 759,250 | G→T | R182L (CGC→CTC) | sucA → | subunit of E1(0) component of 2‑oxoglutarate dehydrogenase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 759,250 | 0 | G | T | 97.1% | 92.8 / NA | 36 | R182L (CGC→CTC) | sucA | subunit of E1(0) component of 2‑oxoglutarate dehydrogenase |
| Reads supporting (aligned to +/- strand): ref base G (0/1); new base T (17/17); total (18/18) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
CAGGAGACCTTCAACGTCGGTTCATTTGCCAGCGGCAAAGAAACCATGAAACTCGGCGAGCTGCTGGAAGCCCTCAAGCAAACCTACTGCGGCCCGATTGGTGCCGAGTATATGCACATTACCAGCACCGAAGAAAAACGCTGGATCCAACAGCGTATCGAGTCTGGTCGCGCGACTTTCAATAGCGAAGAGAAAAAACGCTTCTTAAGCGAACTGACCGCCGCTGAAGGTCTTGAACGTTACCTCGGCGCAAAATTCCCTGGCGCAAAACG > NC_000913/759111‑759382 | cAGGAGACCTTCAACGTCGCTTGATTCGCGAGCGAGACAGAAATCAGGAAACTCGGCGGGCTGCTGGAGGCCCTCAAGCAAACCTACGGCGGCCCGATTGGTGCCGGGTATATGCACATTACCAGCACCGAAGAAAAACTCTg < 2:159405/143‑1 (MQ=255) cTTCAACGTCGGTTCATTTGCCAGCGGCAAAGAAACCATGAAACTCGGCGAGCTGCTGGAAGCCCTCAAGCAAACCTACTGCGGCCCGATTGGTGCCGAGTATATGCACATTACCAGCACCGAAGAAAAACTCTGGATCCAAc > 1:259915/1‑143 (MQ=255) ttCAAGGTCGGTTCATTTGCCAGCGGCAAAGAAACCATGAAACTCGGCGAGCTGCTGGAAGCCCTCAAGCAAACCTACTGCGGCCCGATTGGTGCCGAGTATATGCACATTACCAGCACCGAAGAAAAACTCTGGATCCAACa < 2:458131/143‑1 (MQ=255) cAATGTCGGTTCATTTGCCAGCCGCAAAGAAATCATGAAACTCGGAGAGCTGCTGTAAGCCATCAAGCAATCCTACTTCGGCCCGATTGGTGCCGAGTATATGCACATTTGCAGCACCGAAGAAAAACTCTGGATCCAACAGc < 2:368896/143‑1 (MQ=255) cAACGTCGGTTCATTTGCCAGCGGCAAAGAAACCATGAAACTCGGCGAGCTGCTGGAAGCCCTCAAGCAAACCTACTGCGGCCCGATTGGTGCCGAGTATATGCACATTACCAGCACCGAAGAAAAACTCTGGAGCCAACAGc > 1:380962/1‑143 (MQ=255) cATTTGCCAGCGGCAAAGAAACCATGAAACTCGGCGAGCTGCTGGATGCCCTCAAGCAAACCTACTGCGGCGCGATTGGTGCCGAGTATAGGCACATTACCAGGAGCGAAGAAAAACTCTGGATGAGACAGCGTGTCGAGGCt > 1:403990/1‑143 (MQ=255) aTTTGCCAGCGGCAAAGACACCATGAAACTCGGCGAGCTGCTGGAAGCCCTCAAGCAAACCTACTGCGGCCCGATTGGTGCCGAGTATATGCACATTACCAGCACCGAAGAAAAACTCTGGATCCAACAGCGTATCGAGTCTg < 1:314372/143‑1 (MQ=255) cggtggCAAAGAAACCATGAAACTCGGCGCGCCGCTGAAAGCCCTCAAGCAAACCTCCTGCGGCCCGATTGGTGCCGAGTATATGCACATTACCAGCACCGAAGAAAATCTCTGGATCCAACAGCGTATCGAGTCTGGTcgcg < 1:216035/139‑1 (MQ=255) cAAAGAAACCATGAAACTCGGCGAGCTGCTGGAAGCCCTCAAGCAAACCTACTGCGGCCCGATTGGTGCCGAGTATATGCACATTACCAGCACCGAAGAAACACACTGGATCCAACATCGTATCGAGTCTGGTCGCGCCACtt > 1:541116/1‑143 (MQ=255) aggcaaCCATGAAACTCGGCGGGCTGCGGGAAGCCCTCAATCAAACCTACTGCGGCCCGATTGGTGCCGAGTATATGCACATTACCAGCACCGAAGAAAAACTCTGGATCCAACAGCGTATCGAGTCTGGTCGCGCGACTTTc < 2:348165/139‑1 (MQ=255) cATGAAACTCGGCGAGCTGCTGGAAGCCCTCAAGCAAAACTACTGCGGCACGATTGGTGCCGAGTATATGCACATTACCAGCACCGAAGAAAAACTCTGGATCCAACAGCGTGTCGAGACTGGTCGCGCGCCTGTCAATcgcg > 1:244030/1‑143 (MQ=255) aTGGAACTCGGCGACCTGCGGGAAGCCCTCAAGCTAACCTAATACGGCCCGAGTGGTGCCGAGTATATGCACATTACCAGCACCGAAGAAAAACTCTGGATCCAACAGCGTATCGAGTCTGGTCGCGCGACTTTCAATAGCGa < 2:476028/143‑1 (MQ=255) gAAACTCGGCGAGCTGCTGGAAGCCCTCAAGCAAACCTACTGCGGCCCGATTGGTGCCGAGTATATGCACATTACCAGCACCGAAGAAAAACTCTGGATCCAACAGCGTATCGAGTCTGGTCGCGCGACTTTCAATAGCGAag > 1:62656/1‑143 (MQ=255) gagcccggcggGCTGGTGGGGGTGCTCGAGCATACCTACTGCGGCCCGAGTGGTGCGGCGTATATGCACATTACCAGCGCCGAAGAAAAACGCTGGATACAACAGCGGATCGAGGCTGGTCGCGCGACTTTCAATAGCGAaga < 2:516814/138‑1 (MQ=255) gcGGCGAGCTGCTGGAAGCCCTCAAGCAAACCTACGGCGGCCCGATTGGTGCCGAGTATATGCACATTACCAGCACCGAAGAAAAACTCTGGATCCAACAGCGTATCGAGTCTAGTCGCGCGACTTTCAATAGCGAAGAGaaa < 2:655119/142‑1 (MQ=255) cGAGCTGCTGGAAGCCCTCAAGCAAACCTACTGCGGCCCGATTGGTGCCGAGTATATGCACATTACCAGCACCGAAGAAAAACTCTGGATCCAACAGCGTATCGAGCCTCGTCGCGCGACTGTCAATAGCGAAGAGgaaaaaa > 2:118302/1‑142 (MQ=255) ggAAGCCCTCAAGCAAACCTACTGCGGCCCGATTGGTGCCGAGTATATGCACATTACCAGCACCGAAGAAAAACTCTGGATCCAACAGCGTATCCAGTCTGGTAGCGCGACTTTCAATAGAGAAGAGAAAAAACGCTTCTTaa > 2:243465/1‑143 (MQ=255) gAAGCCCTCAAGCAAACCTACTGCGGCCCGATTGGTGCCGAGTATATGCACATTACCAGCACCGAAGAAAAACTCTGGATCCAACAGCGCATCGAGTCTGGTCGCGCGACTTTCAATAGCGAAGAGAAAAAACGCTTCTTAAg > 2:699347/1‑143 (MQ=255) ccccccaGCAACCCTACCGCGGCCCGATTGGTGCCGAGTATATGCACATTACCAGCACCGAAGAAAAACTCTGGATTCAACAGCGTATCGAGTCTGGTCGCGCGACTTTCAATAGCGAAGAGAAAAAACGCTTCTTAAGCGaa < 1:517637/137‑1 (MQ=255) cccTCAAGCAAACCTACTGCGGCCCGATTGGTGCCGAGTATATGCACATTACCAGCACCGAAGAAAAACTCTGGATCCCACAGCGTCTCGAGTCTGGTCGCGCGACTTTCCATAGCGAAGAGAAAAAACGCGCCTTAAGCGaa > 1:65101/1‑143 (MQ=255) cTCAAGCAAACCTACTGCGGCCCGATTGGTGCCGAGTATATGCACATTACCAGCACCGAAGAAAAACTCTGGATCCAACAGCGTATCGAGTCTGGTCGCGCGACTTTCAATAGCGAAGAGAAAAAACGCTTCTTAAGCGGACt > 1:81526/1‑143 (MQ=255) aaGCAAACCTACTGCGGCCCGATTGGTGCCGAGTATATGCACATTACCAGCACCGAAGAAAAACTCTGGATCCAACAGCGTATCGAGTCTGGTCGCGCGACTTTCAATAGCGAAGAGAAAAAACGCTTCTTAAGCGAACTGAc < 1:542781/143‑1 (MQ=255) aGCAAACCTACTGCGGCCCGATTGGTGCCGAGTATATGCACATTACCAGCACCGAAGAAAAACTCTGGATCCAACAGCGTATCGAGTCTGGTCGCGCGACTTTCAATAGCGACGAGAAAAAACGCTTCTTAAGCGAACTGAcc > 1:308084/1‑143 (MQ=255) aCCTACTGCGGCCCGATTGGTGCCGAGTATATGCACATTACCAGCACCGAAGAAAAACTCTGGATCCAACAGCGTATCGAGTCTGGTCGCGCGACTTTCAATAGCGAAGAGAAAAAACGCTTCTTAAGCGAACTGACcgccgc < 2:259915/143‑1 (MQ=255) aCTGCGGGCCGATTGGGGCCGAGTATATGCACATTAGCGGGAACGAAGAAAATCTCTGGATCCAACAGCGTATCGAGTCTGGTCGCGCGACTTTCAATAGCGAAGAGAAAAAACGCTTCTTAAGCGAACTGACCGCCGCTGaa < 2:353265/143‑1 (MQ=255) aTTGGTGCCGAGTATATGCACATTACCAGCACCGAAGAAAAACTCTGGATCCAACAGCGTATCGAGTCTGGTCGCGCGACTTTCAATAGCCAAGAGAAAAAACGCTTCTTAAGCGAACTGACCGCCGCGGAAGGGCGTGAGCg > 2:258588/1‑143 (MQ=255) aTTGGTGCCGAGTATATGCACATTACCAGCACCGAAGAAAAACTCTGGATCCAACAGAGTATCGGGTCTGGTCGCGCGACTTTCAATAGCGAAGAGAAAAAACGCTTCTTAAGCGCAATGACCGCCGTTGAAGGTGTTGAGCg > 1:330384/1‑143 (MQ=255) ggTGCCGAGTATATCCACAGTACGAGCCCCGAAGAAAACCTCTGGATCCAACAGCGTATCGAGTCTGGTCGCGCGACTTTCAATAGCGAAGAGAAAAAACGCTTCTTAAGCGAACTGACCGCCGCTGAAGGTCTTGAACGTTa < 2:330384/143‑1 (MQ=255) gTGCCGAGTATATGCACATTACCAGCACCGAAGAAAAAATATGGATACAAGAGGGTATCGAGTCTGGTCGCGCGACTTTCAATAGCGAAGAGAAAAAACGCTTCTTAAGCGAACTGACCGCCGCTGTAGGTCTGGAACGttgc > 1:338597/1‑141 (MQ=255) cGAGTATATGCACATTACCCGCACCGAAGAAAAACTCTGGATCCAACAGCGTATCGAGTCTGGTCGCGCGACTTTCAATAGCGAAGAGAAAAAACGCTTCTTAAGCGAACTGACCGCCGCTGAAGGTCTTGAACGTTACCTCg < 1:112099/143‑1 (MQ=255) cGAGTATATGCACATTACCAGCACCGAAGAAAAACTCTGGATCCAACAGCGTATCGAGTCTGGTCGCGCGACTTTCAATAGCGAAGAGAAAAAACGCTTCTTAAGCGAACTGACCGCCGCTGAAGGTCTTGAACGTTACCTCg > 1:447169/1‑143 (MQ=255) caTTACCAGCACCGAAGAAAAACTCTGGATCCAACAGCGTATCGAGTCTGGTCGCGCGACTTTCAATAGCGAAGAGAAAAAACGCTTCTTAAGCGAACTGACCGCCGCTGAAGGTCTTGAACGTTACCTCGGCGCAAAATTcc < 2:62656/143‑1 (MQ=255) ttgccAGCACCGAAGAATAGCTCTGGATCCAACAGCGTATCGAGTCTGGTCGCGCGACTTTCAATAGCGAAGAGAAAAAACGCTTCTTAAGCGAACTGACCGCCGCTGAAGGTCTTGAACGTTACCTCGGCGCAAAATTCCCt < 2:648342/140‑1 (MQ=255) cAGCACCGAAGAAAAACTCTGGATCCAACAGCGTATCGAGTCTGGTCGCGCGACTTTCAATAGCGAAGAGAAAAAACGCTTCTTAAGCGAACTGACCGCCGCTGAAGGTCTTGAACGTTACCTCGGCGCAAAATTCCCTGgcg > 1:288487/1‑143 (MQ=255) gaagaaACGCTCTGGATCCAACAGCGTATCGAGTCTGGTCGCGCGACTTTCAATAGCGAAGAGAAAAAACGCTTCTTAAGCGAACTGACCGCCGCTGAAGGTCTTGAACGTTACCTCGGCGCAAAATTCCCTGGCGCAAAACg < 2:709936/143‑1 (MQ=255) gaagaaAAACTCTGGATCCAACAGCGTATCGAGTCAGGTCACGCGACTTTCAATAGCCAAGAGAAAAAACGCTTCTTAAGCGAACTGACCGCCGCTGAAGGTCTTGACCGTTACCTCGGCGCAAAATTCCCTGGCGCAAAACg > 2:607945/1‑143 (MQ=255) | CAGGAGACCTTCAACGTCGGTTCATTTGCCAGCGGCAAAGAAACCATGAAACTCGGCGAGCTGCTGGAAGCCCTCAAGCAAACCTACTGCGGCCCGATTGGTGCCGAGTATATGCACATTACCAGCACCGAAGAAAAACGCTGGATCCAACAGCGTATCGAGTCTGGTCGCGCGACTTTCAATAGCGAAGAGAAAAAACGCTTCTTAAGCGAACTGACCGCCGCTGAAGGTCTTGAACGTTACCTCGGCGCAAAATTCCCTGGCGCAAAACG > NC_000913/759111‑759382 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |