Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	800,807	G→T	A17S (GCG→TCG)	ybhH →	putative isomerase YbhH

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	800,807	0	G	T	100.0%	126.3 / NA	41	A17S (GCG→TCG)	ybhH	putative isomerase YbhH
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base T (19/22);  total (19/22)

TTATTAATCGTTGAGCTAAAGTCATTAGAGATGCTTTGCCCTTAATGTAACCATATCGCAATAAGTTATGTTTTTAAATTGAGGGCATTATTATGAAAAAAATACCCTGCGTGATGATGCGAGGTGGAACCTCGAGGGGCGCGTTCCTGTTAGCGGAACATTTACCCGAAGATCAAACGCAGCGCGATAAAATATTGATGGCAATTATGGGTTCCGGTAACGATCTGGAAATTGACGGTATTGGCGGCGGTAATCCACTGACCAGTAAAGTCGC  >  NC_000913/800667‑800940                                                                                                                                             |                                                                                                                                      ttattaATCGTTGAGCTAAAGTCATTAGAGATGCTTTGCCCTTAATGTAACCATATCGCAATAAGTTATGTTTTTAAATTGAGGGCATTATTATGAAAAAAATACCCTGCGTGATGATGCGAGGTGGAACCTCGAGGGGctcg                                                                                                                                     >  2:27359/1‑143 (MQ=255)        tCGTTGATCTAAAGTCATTAGAGATGCTTTGCCCTTAAGTTAACCATATAGCAATAAGTTATGTTTTTAAATTGAGGGCATTATTATGAAAAAAATACCCTGCGTGATGATGCGAGGTGGAACCTCGAGGGGCTCGTTCCTGt                                                                                                                              <  1:610275/143‑1 (MQ=255)              aGCTAAAGTCATTAGAGATGCTTTGCCCTTAATGTAACCATATCGCAATAAGTTATGTTTTTAAATTGAGGGCATTATTATGAAAAAAATACCCTGCGTGATGATGCGAGGTGGAACCTCGAGGGGCTCGTTCCTGTTAGCgg                                                                                                                        <  1:621914/143‑1 (MQ=255)              aGCTAAAGTCATTAGAGATGCTTTGCCCTTAATGTAACCATATCGCAATAAGTTATGTTTTTAAATTGAGGGCATTATTATGAAAAAAATACCCTGCGTGATGATGCGAGGTGGAACCTCGAGGGGCTCGTTCCTGTTAGCgg                                                                                                                        <  1:621829/143‑1 (MQ=255)              aGCTAAAGTCATTAGAGATGCTTTGCCCTTAATGTAACCATATCGCAATAAGTTATGTTTTTAAATTGAGGGCATTATTATGAAAAAAATACCCTGCGTGATGATGCGAGGTGGAACCTCGAGGGGCTCGTTCCTGTTAGCgg                                                                                                                        >  1:755438/1‑143 (MQ=255)               gCTAAAGTCATTAGAGATGCTTTGCCCTTAATGTAACCATATCGCAATAAGTTATGTTTTTAAATTGAGGGCATTATTATGAAAAAAATACCCTGCGTGATGATGCGAGGTGGAACCTCGAGGGGCTCGTTCCTGTTAGCGGa                                                                                                                       >  1:59903/1‑143 (MQ=255)               gCTAAAGTCATTAGAGATGCTTTGCCCTTAATGTAACCATATCGCAATAAGTTATGTTTTTAAATTGAGGGCATTATTATGAAAAAAATACCCTGCGTGAGGATGCGAGGTGGAACCTCGAGGGGCTCGTTCCTGTTAGCGGa                                                                                                                       >  1:278537/1‑143 (MQ=255)                 tAAAGTCATTAGAGATGCTTTGCCCTTAATGTAACCATATCGCAATAAGTTATGTTTTTAAATTGAGGGCATTAATATGAAAAAAATACCCTGCGTGATGATGCGAGGTGGAACCTCGAGGGGCTCGTTCCTGCTAGCGGCAc                                                                                                                     >  1:544570/1‑143 (MQ=255)                  aaaGTCATTAGAGATGCTTTGCCCTTAATGTAACCATATCGCAATAAGTTATGTTTTTAAATTGAGGGCATTATTATGAAAAAAATACCCTGCGTGATGATGCGAGGTGGAACCTCGAGGGGCTCGTTCCTGTTAGCGGAACa                                                                                                                    >  1:710686/1‑143 (MQ=255)                      tCATTAGAGCTGCTTTGCCCTTAATGTAACCATATCGCAATAAGTTATGTTTTTAAATTGAGGGCATTATTATGAAAAAAATACCCTGCGTGATGATGCGAGGTGGAACCTCGAGGGGCTCGTTCCTGTTAGCGGAACATTTa                                                                                                                <  2:460685/143‑1 (MQ=255)                                   cttGCCCGTAATGGAACCATATCGCAATAAGTTATGTTTTTAAATTGAGGGCATTATTATGAAAAAAATACCCTGCGTGATGATGCGAGGTGGAACCTCGAGGGGCTCGTTCCTGTTAGCGGAACATTTACCCGAAGATCaaa                                                                                                   <  1:379434/142‑1 (MQ=255)                                       ccattAATGTAACCATATCGCAATAAGTTATGTTTTTAAATTGAGGGCATTATTATGAAAAAAATACCCTGCGTGATGATGCGAGGTGGAACCTCGAGGGGCTCGTTCCTGTTAGCGGAACATTTACCCGAAGATCAAACGCa                                                                                               <  1:252709/140‑1 (MQ=255)                                         cTTAATGTAACCATATCGCAATAAGTTATGTTTTTAAATTGAGGGCATTATTATGAAAAAAATACCCTGCGTGATGATGCGAGGTGGAACCTCGAGGGGCTCGTTCCTGTTAGCGGAACATTTACCCGAAGATCAAACGCAgc                                                                                             >  1:4942/1‑143 (MQ=255)                                          ttAATGTAACCATATCGTAATAAGTTATGTTTTTAAATTGAGGGCATTATTATGAAAAAAATACCCTGCGTGATGATGCGAGGTGGAACCTCGAGGGGCTCGTTCCTGTTAGCGGAACATTTACCCGCAGATCAAACGCAgcg                                                                                            <  2:673406/143‑1 (MQ=255)                                             aTGTAACCATATCGCAATAAGTTATGTTTTTAAATTGAGGGCATTATTATTAAAAAAATACCCTGCGTGATGATGCGAGGTGGAACCTCGAGGGGCTCGTTCCTGTTAGCGGAACATTTACCCGAAGATCAAACGCAGCGCGa                                                                                         >  1:339605/1‑143 (MQ=255)                                                         ggaaataatTTATGTGTTGAAATTGATTGCATTATTATGAAAAAAATACCCTGCGTGATGATGCGAGGTGGAACCTCGAGGGGCTCGTTCCTGTTAGCGGAACATTTACCCGAAGATCAAACGCAGCGCGATAAAATATTGAt                                                                             <  2:669787/140‑1 (MQ=255)                                                           cAATAAGTTATGTTTTTAAATTGAGGGCATTATTATGAAAAAAATACCCTGCGTGATGATGCGAGGTGGAACCTCGAGGGGCTCGTTCCTGTTAGCGGAACATTTACCCGAAGATCAAACGCAGCGCGATAAAATATTGATgg                                                                           <  1:775449/143‑1 (MQ=255)                                                              tAAGTTATGTTTTTAAATTGAGGGCATTATTATGAAAAAAATACCCTGCGTGATGATGCGAGGTGGAACCTCGAGGGGCTCGTTCCTGTTAGCGGAACATTTACCCGAAGATCAAACGCAGCGCGATAAAATATTGATGGCaa                                                                        >  2:520718/1‑143 (MQ=255)                                                                  ttATGTTTTTAATTTGAGGGCATTATTATGAGAAAAATACCGTGCGTGATGATGCGAGGTGGAACCGCGAGGGGCTCGTTCCTGTTAGCGGAACATTTACCCGAAGATCAAACGCAGCGCGATAAAATATTGATGGCAATTAt                                                                    <  1:389142/143‑1 (MQ=255)                                                                  ttATGTTTTTAAATTGAGGGCATTATTATGAAAAAAATACCCTGCGTGATGATGCGAGGTGGAACCTCGAGGGGCTCGTTCCTGTTAGCGGAACATTTACCCGAAGATCAAACGCAGCGCGATAAAATATTGATGGCAATTAt                                                                    >  1:531303/1‑143 (MQ=255)                                                                    aTGTTTTTAAATTGAGGGCATTATTATGAAAAAAATACCCTGCGTGATGATGCGAGGTGGAACCTCGAGGGGCTCGTTCCTGTTAGCGGAACATTTACCCGAAGATCAAACGCAGCGCGATAAAATATTGATGGCAATTATgg                                                                  <  2:755438/143‑1 (MQ=255)                                                                     tGTTTTTAAATTGAGGGCATTATTATGAAAAAAATACCCTCCTCGATGATGCGAGGTGAAACCTCGAGAGGCTCGATCCTATTAGCGGAACATTTACCCGAAGATCAAACGCAGCGCGATAAAATATTGATGGCAAATATggg                                                                 >  1:800669/1‑143 (MQ=255)                                                                        tgttACATTGGGGGCATTATTATGGAAACAATACCCTCAGTGACAATGCGAGGTGGAACCTCGAGGGGCTCGGTCCTGTTAGCGGAACATTTACCCGAAGATCAAACGCAGCGCGATAAAATATTGCTGGCAATTATGGGTTc                                                              <  2:574989/141‑1 (MQ=255)                                                                         tttAAATTGAGGGCATTATTATGAAAAAAATACCCTGCGTGATGATGCGAGGTGGAACCTCGAGGGGCTCGTTCCTGTTAGCGGAACATTTACCCGAAGATCAAACGCAGCGCGATAAAATATTGATGGCAATTATGGATTcc                                                             >  2:695868/1‑143 (MQ=255)                                                                         tttAAATTGAGGGCATTATTATGAAAAAAATACCCTGCGTGATGATGCGAGGTGGAACCTCGAGGGGCTCGTTCCTGTTAGCGGAACATTTAACCGAAGATCAAACGCAGCGCGATAAAATATTGATGGCAATTATGGGTTcc                                                             >  1:730803/1‑143 (MQ=255)                                                                              aTTGAGGGCATTATTATGAAAAAAATACCCGGCGTGATGATGCGAGGTGGAACCTCGAGGGGCTCGTTCCTGTTAGCGGAACATTTACCCGAAGATCAAACGCAGCGCGATAAAATATTGATGGCAATTATGGGTTCCGGTaa                                                        <  1:360452/143‑1 (MQ=255)                                                                                   gggCATTATTATGAAAAAAATACCCTGCGTGATGATGCGAGGTGGAACCTCGAGGGGCTCGTTCCTGTTAGCGGAACATTTACCCGAAGATCAAACGCAGCGCGATAAAATATTGATGGCAATTATGGGTTCCGGTAACGATc                                                   <  1:531927/143‑1 (MQ=255)                                                                                        ttattaTGAAAAAAAGACCCTGCGTGATGGTGCGAGGTGGAACCTCGAGGGGCTCGTTCCTGTTAGCGGAACATTTACCCGAAGATCAAACGCAGCGCGATAAAATATTGATGGCAATTATGGGTTCCGGTAACGATCTGGaa                                              <  2:618906/143‑1 (MQ=255)                                                                                          attatGAAAAAAATACCCTGCGTGATGATGCGAGGTGGAACCTCGAGGAGCTCGTTCCTGTTAGCGGAACATTTACCCGAAGATCAAACGCAGCGCGATAAAATATTGATGGCGATTATGGGTTCCGGTAACGATCTGGAAAt                                            >  1:110395/1‑143 (MQ=255)                                                                                           tgatGAAAAAAATACCCTGCGTGATGATGCGAGGTGGAACCTCGAGGGGCTCGTTCCTGTTAGCGGAACATTTACCCGAAGATCAAACGCAGCGCGATAAAATATTGATGGCAATTATGGGTTCCGGTAACGATCTGGAAAtt                                           <  2:751018/141‑1 (MQ=255)                                                                                             atGAAAAAAATACCCTGCGTGATGATGCGAGGTGGAACCTCGAGGGGCTCGTTCCTGTTAGCGGAACATTTACCCGAAGATCAAACGCAGCGCGATAAAATATTGATGTCAATTATGGGTTCCGGTAACGATCTGGAAATTGa                                         >  1:81686/1‑143 (MQ=255)                                                                                             atGAAAAAAATACCCTGCGTGATGATGCGAGGTGGAACCTCGAGGGGCTCGTTCCTGTTAGCGGAACATTTACCCGAAGATCAAACGCAGCGCGATAAAATATTGATGGCAATTATGGGTTCCGGTAACGATCTGGAAATTGa                                         >  1:525766/1‑143 (MQ=255)                                                                                              tGAAAAAAATACCCCGCATGATGATGCGAGGTGGAACCTCGAGGGGCTCGTTCCTGTTAGCGGAACATTTACCCGAAGATCAAACGCAGCGCGATAAAATATTGATGGCAATTATGGGTTCCGGTAACGATCTGGAAATTGAc                                        <  1:45589/143‑1 (MQ=255)                                                                                                 aaaaaaTACCCTGCGTGATGATGCGAGGTGGAACCTCGAGGGGCTCGTTCCTGTTAGCGGAACATTTACCCGAAGATCAAACGCAGCGCGATAAAATATTGATGGCAATTATGGGTTCCGGTAACGGTCTGGAAATTGACGGt                                     >  1:90576/1‑143 (MQ=255)                                                                                                   aaaaTACCCTGCGTGATGATGCGAGGTGGAACCTCGAGGGGCTCGTTCCTGTTAGCGGAACATTTACCCGAAGATCAAACGCAGCGCGATAAAATATTGATGGCAATTATGGGTTCCGGTAACGATCTGGAAATTGACGGTAt                                   <  1:763075/143‑1 (MQ=255)                                                                                                               gTGATGATGCGAGGTGGAACCTCGAGGGGCTCGTTCCTGGTAGCGGAACATTTACCCGAAGATCAAACGCAGCGCGATAAAATATTGATGGCAATTATGGGTTCCGGTAACGATCTGGAAATTGACGGTATTGGCGGCGGTaa                       <  1:27359/143‑1 (MQ=255)                                                                                                                   tgattCGAGGTGGATCCTCGTGTGGCTCATTCCTGTTAGCGGAACCTTTACCCGAAGATCAAACGCAGCGCGATAAAATATTGATGGCAATTATGGGTTCCGGTAACGATCTGGAAATTGACGGTATTGGCGGCGGTAATCCa                   <  1:131935/143‑1 (MQ=255)                                                                                                                     gtgCGAGGAGGAACCTCGAGGGGCTCCCTCCTAGTGGCTGAATATCTACCCGAAGATCAAACGCAGCGCGATAAAATATTGATGTCAATTATGGGTTCCGGTAACGATCTGGAAATTGACGGTATTGGCGGCGGTAATCCACt                 <  1:752910/142‑1 (MQ=255)                                                                                                                         gAGGTGGAACCTCGAGGGGCTCGGTCCTGTTAGCGGAACATTTACCCGAAGATCAAACGCAGCGCGAAAAAATATTGATGGCAATTATGGGTTCCGGTAACGATCTAGAAATTGACGGTATTGGCGGCGGAACTCCCCTGAcc             >  2:727651/1‑143 (MQ=255)                                                                                                                          aGGTGGAACCTCGAGGGGCTCGTTCCTGTTAGCGGAACATTTACCCGAAGATCAAACGCAGCGCGATAAAATATTGATGGCAATTATGGGTTCCGGTAACGATCTGGAAATTGACGGTATTGGCGGCGGTAATCCACTGACCa            >  1:676578/1‑143 (MQ=255)                                                                                                                               gAACCTCGAGGGGCTCGTTCCTGTTAGCGGAACATTTACCCGAAGATCAAACGCAGCGCGATAAAATATTGATGGCAATTATGGGTTCCGGCAACGATCTGGAAATTGACGGTATTGGCGGCGGTAATCCACTGACCAGTaag       >  1:125993/1‑142 (MQ=255)                                                                                                                                    tCGAGGGGCTCGTTCCTGTTAGCGGAACATTTACCCGAAGATCAAACGCAGCGCGATAGAATATTGATGGCAATTATGGGTTCCGGTAACGATCTGGAAATTGACGGTATTGGCGGCGGTAATCCACTGACCAGTAAAGTCGc  <  2:798/143‑1 (MQ=255)                                                                                                                                             |                                                                                                                                      TTATTAATCGTTGAGCTAAAGTCATTAGAGATGCTTTGCCCTTAATGTAACCATATCGCAATAAGTTATGTTTTTAAATTGAGGGCATTATTATGAAAAAAATACCCTGCGTGATGATGCGAGGTGGAACCTCGAGGGGCGCGTTCCTGTTAGCGGAACATTTACCCGAAGATCAAACGCAGCGCGATAAAATATTGATGGCAATTATGGGTTCCGGTAACGATCTGGAAATTGACGGTATTGGCGGCGGTAATCCACTGACCAGTAAAGTCGC  >  NC_000913/800667‑800940

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑