Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
JC	NC_000913	2,392,615	Δ3 bp	57.0%	coding (425‑427/1530 nt)	nuoM ←	NADH:quinone oxidoreductase subunit M

New junction evidence
		seq id	position	reads (cov)	reads (cov)	score	skew	freq	annotation	gene	product
*	?	NC_000913	2392618 =	11 (0.240)	14 (0.330)	14/244	NT	57.0%	coding (424/1530 nt)	nuoM	NADH:quinone oxidoreductase subunit M
	?	NC_000913	= 2392614	11 (0.260)					coding (428/1530 nt)	nuoM	NADH:quinone oxidoreductase subunit M

GACCGGTCTGCTCGGTGTGCTGGCGGTACTATGTTCGTGGAAAGAGATCGAAAAATATCAGGGCTTCTTCCACCTCAACCTGATGTGGATCCTGGGCGGCGTTATCGGCGTGTTCCTTGCCATCGACATGTTCCTGTTCTTCTTCT‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  <  NC_000913/2392763‑2392618 ‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑ttcttcttctGGGAAATGATGCTGGTGCCGATGTACTTCCTGATCGCACTGTGGGGGCATAAAGCCTCTGACGGTAAAACGCGTATCACGGCGGCAACCAAGTTCTTCATTTACACCCAGGCGAGTGGCCTGGTGATGTTGATTGCCATCCT  <  NC_000913/2392614‑2392473                                                                                                                                                                                                                                                                                                  GACCGGTCTGCTCGGTGTGCTGGCGGTACTATGTTCGTGGAAAGAGATCGAAAAATATCAGGGCTTCTTCCACCTCAACCTGATGTGGATCCTGGGCGGCGTTATCGGCGTGTTCCTTGCCATCGCCATGTCCCTGCTCTTCc                                                                                                                                                   >  2:598342/1‑142  ACCGGTCTGCTCGGTGTGCTGGCGGTACTATGTTCGTGGAAAGAGATCGAAAAATATCAGGGCTTCTTCCACCTCAACCTGATGTGGATCCTGGGCGGCGTTATCGGCGTGTTCCTTGCCATCGACATGTTCCTGTTCTTCTT                                                                                                                                                  >  2:21518/1‑143   CCGGTCTGCTCGGTGTGCTGGCGGTACTATGTTCGTGGAAAGAGATCGAAAAATATCAGGGCTTCTTCCACCTCAACCTGATGTGGATCCTGGGCGGCGTTATCGGCGTGTTCCTTGCCATCGACATGTTCCTGTTCTTCTTC                                                                                                                                                 >  1:93501/1‑143    CGGTCTGCTCGGTGTGCTGGCGGTACTATGTTCGTGGAAAGAGATCGAAAAATATCAGGGCTTCTTCCACCTCAACCTGATGTGGATCCTGGGCGGCGTTATCGGCGTGTTCCTTGCCATCGACATGTTCCTGTTCTTCTTCT                                                                                                                                                >  1:288098/1‑143     GGTCTGCCCGGTGTGCTGGCGGTACTATGGTCGTGGAAAGAGATCGAAAAATATCAGGGCTTCTTCCACCTCAACCTGATGTGGATCCTGGGCGGCGTTATCGGCGTGTTCCTTGCCATCGACATGTTCCTGTTCTTCTTCTG                                                                                                                                               <  1:761343/143‑1         TGCTCGGTGTGCTGGCGGTACTATGTTCGTGGAAAGAGATCGAAAAATATCAGGGCTTCTTCCACCTCAACCTGATGTGGATCCTGGGCGGCGTTATCGGCGTGTTCCTTGCCATCGACATGTTCCTGTTCTTCTTCTGGGAA                                                                                                                                           >  1:745871/1‑143          GCTCGGTGTGCTGGCGGTACTATGTTCGTGGAAAGAGATCGAAAAATATCAGGGCTTCTTCCACCTCAACCTGATGTGGATCCTGGGCGGCGTTATCGGCGTGTTCCTTGCCATCGACATGTTCCTGTTCTTCTTCTGCGAAA                                                                                                                                          >  2:800406/1‑143                    CTGGCGGTACTATGTTCGTGGAAAGAGATCGAAAAATATCAGGGCTTCTTCCACCTCAACCTGATGTGGATCCTGGGCGGCGTTATCGGCGTGTTCCTTGCCATCGACATGTTCCTGTTCTTCTTCTGGGAAATGATGCTGGT                                                                                                                                >  1:217716/1‑143                       GCGGTACTATGTTCGTGGAAAGAGATCGAAAAATATCAGGGCTTCTTCCACCTCAACCTGATGTGGATCCTGGGCGGCGTTATCGGCGTGTTCCTTGCCATCGACATGTTCCTGTTCTTCTTCTGGGAAATGATGCTGGTGCC                                                                                                                             >  1:249966/1‑143                                 GTTCGTGGAAAGAGATCGAAAAATATCAGGGCTTCTTCCACCTCAACCTGATGTGGATCCTGGGCGGCGTTATCGGCGTGTTCCTTGCCATCGACATGTTCCTGTTCTTCTTCTGCGCAATGATGCTGGTGCCGCTGTAATTC                                                                                                                   >  2:383734/1‑143                                  TTCGTGGAAAGAGATCGAAAAATATCAGGGCTTCTTCCACCTCAACCTGATGTGGATCCTGGGCGGCGTTATCGGCGTGTTCCTTGCCATCGACATGTTCCTGTTCTTCTTCTGGGAAATGATGCTGGTGCCGATGTACTTCC                                                                                                                  >  1:385115/1‑143                                    CGTGGAAAGAGATCGAAAAATATCAGGGCTTCTTCCACCTCAACCTGATGTGGATCCTGGGGGGCGTTATCGGCGTGTTCCTTGCCATCGACATGTTCCTGCTCTTCTTCGGGGAAGTGACGCTGGTGCAGATGTACTaactg                                                                                                                >  2:176524/1‑138                                      TGGAAAGAGATCGAAAAATATCAGGGCTTCTTCCACCTCAACCTGATGTGGATCCTGGGCGGCGTTATCGGCGTGTTCCTTGCCATCGACGTGTTCCTGTTCTTCTTCTGGGAAATGATGCTGGTGCCGATGTACTTCCTGct                                                                                                              >  2:613778/1‑141                                        GAAAGAGATCGAAAAATATCAGGGCTTCTTCCACCTCAACCTGATGTGGATCCTGGACGGCGTTATCGGCGTGTTCCTTGACATCGACATGTTCCTGTTCTTCTTCTGGGAAATGATGCTGGTGCCGATGAACTTCCTGATCG                                                                                                            >  1:448748/1‑143                                                   AAAAATATCAGGGCTTCTTCCACCCCAACCTGATGTGGATCCTGGGCGGCGTTATCGGCGTGTTCCTTGCCATCGACATGTTCCTGTTCTTCTTCTGGGAAATGATGCTGGTGCCGATGTACTTCCTGATCGCACTGTGGGGG                                                                                                 <  1:744569/143‑1                                                            AGGGCTTCTTCCACCTCAACCTGATGTGGATCCTGGGCGGCGTTATCGGCGTGTCCCTTGCCATCGCCACGTTCCTGTTCTTCTGCCGGGAAATGAGGCTCGTGCCGACGTACTTCCTGATCGCACTGTGGGGGCATCAGGCC                                                                                        >  2:201020/1‑143                                                                                           CCTGGGCGGCGTTATCGGCGTGTTCCTTGCCATCGACATGTTCCTGTTCTTCTTCTGGGAAATGATGCTGGTGCCGATGTACTTCCTGATCGCACTGTGGGGGCATAAAGCCTCTGACGGTAAAACGCGTATCACGGCGGCAA                                                         <  2:745871/143‑1                                                                                                                         CATCGACATGTTCCTGTTCTTCTTCTGGGAAATGATGCTGGTGCCGATGTACTTCCTGATCGCACTGTGGGGGCATACAGCCTCTGACGGTAAAACGCGTATCACGGCGGCAACCAAGTTCTGCATTTACACCCAGGCaggtg                           >  2:27339/1‑138                                                                                                                           TCGACATGTTCCTGCTCTTCTTCTGGGATATGGTGCTGGTGCAGTGGGAGTTCCTGATCGCACTGTGGGGGCATAGAGCCTCTTTCGGTAAAACGCGTATCACGGCGGCAACCAAGTTCTTCATTTACACCCAGGCGAGTGGC                         <  2:185231/143‑1                                                                                                                                agGTTCCTGTTCTTCTTCTGGGAAATGATGCTGGTGCCGATGTACTTCCTGATCGCACTGTGGGGGCATAAAGCCTCTGACGGTAAAACGCGTATCACGGCGGCAACCAAGTTCTTCATTTACACCCAGGCGAGTGGCCTGGT                    <  1:21518/141‑1                                                                                                                                    TCCTGTTCTTCTTCTGGGAAATGATGCTGGTGCCGATGTACTTCCTGATCGCACTGTGGGGGCATAAAGCATCTGACGGTAAAACGCGTATCACGGGGGCAACCAAATTCTTCATTTACACCCAGCCGAGTGGCCTGGTGGTG                >  2:453265/1‑143                                                                                                                                      CTGTTCTTCTTCTGGGAAATGATGCTGGTGCCGATGTACTTCCTGATCGCACTGTGGGGGCATAAAGCCTCTGACGGTAAAACGCGTCTCACGGCGGGAACCAAGTTCTTCATTTACACCCAGGCGAGTGGCCTGGTGATGTg              >  1:506958/1‑142                                                                                                                                          tcgTCTTCTGGGAAATGATGCTGGTGCCGATGTACTTCCTGATCGCACTGTGGGCGCACACAGTCGCTGACGGTAAAACGCGTATCACGGCGGCAACCAAGTTCTTCATTTACACCCAGGCGAGTGGCCTGGTGATGTTGATT          <  1:652087/140‑1                                                                                                                                             TCTTCTGGGAAATGATGCTGGTGCCGATGTACTTCCTGATCGCACTGTGGGGGCATAAAGCCTCTGACGGTAAAACGCGTATCACGGCGGCAACCAAGTTCTTCATTTACACCCAGGCGAGTGGCCTGGTGATGTTGATTGCC       <  1:714884/143‑1                                                                                                                                                  TGGGAAATGATGCTGGTGCCGATGTACTTCCTGATCGCACTGTGGGGGCATAAAGCCTCTGACGGTCGAACGCGTATCACGGCGGCAACCAAGTTCTTCATTTCCACCCAGGCGAGGGGCCTAGTGATGTGCATTGCacgcct  >  2:508377/1‑137                                                                                                                                                                                                                                                                                                  GACCGGTCTGCTCGGTGTGCTGGCGGTACTATGTTCGTGGAAAGAGATCGAAAAATATCAGGGCTTCTTCCACCTCAACCTGATGTGGATCCTGGGCGGCGTTATCGGCGTGTTCCTTGCCATCGACATGTTCCTGTTCTTCTTCT‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  <  NC_000913/2392763‑2392618 ‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑ttcttcttctGGGAAATGATGCTGGTGCCGATGTACTTCCTGATCGCACTGTGGGGGCATAAAGCCTCTGACGGTAAAACGCGTATCACGGCGGCAACCAAGTTCTTCATTTACACCCAGGCGAGTGGCCTGGTGATGTTGATTGCCATCCT  <  NC_000913/2392614‑2392473

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.