Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 314,258 G→A 10.2% intergenic (‑453/‑99) ykgP ← / → eaeH putative oxidoreductase/putative porin domain‑containing protein EaeH

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913314,2580GA10.2% 117.7 / 5.3 49intergenic (‑453/‑99)ykgP/eaeHputative oxidoreductase/putative porin domain‑containing protein EaeH
Reads supporting (aligned to +/- strand):  ref base G (26/18);  new base A (3/2);  total (29/20)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.76e-01

CTTGCACAAAAAGCTAAATTTTCCCCCATAAATAAAAATATAATCCCGCGCCCAACCACCTGATGAGTGGCTATAGGCACTGGATATATTAGGTGGCGGTGCACTTTCTTACATAAAGGTATTTCCTTTTCTGCGGAAAAGGAAATCGGGAAATCCCCGGTTTTTCTGACAAGCAGACGCCATTATTTGTGTCTGCCTATGTTCGTTAATTCGTTCATCAGGAAATTATCTCAATGTCACATTATAAAACAGGTCATAAACAACCACGATTTCGTTA  >  NC_000913/314124‑314400
                                                                                                                                      |                                                                                                                                              
cTTGCACAACAAGTTAAATTTTCCCCCATAAATTAAAATATAATCCCGACCCCAACCTCCTGATGAGTGTCTATCGCCACTGTATATATTAGGTGGCGGTGCACTTTCTTACATAAAGGTATTTCCTTTTCTGCAGAAAAGGa                                                                                                                                        <  2:181617/143‑1 (MQ=255)
 ttGCACAAAAAGCTAAATTTTCCCCCATAAATAAAAATATAATCCCGCGCCCAACCACCTGATGGGTGGCTATAGGCACTGGATATATTAGGTGGCGGTGCACTTTCTTACATAAAGGTATTTCCTTTTCCGCAGAAAAGGaa                                                                                                                                       <  1:508888/143‑1 (MQ=255)
 ttGCACAAAAAGCTAAATTTTCCCCCATAAATAAAAATATAATCCCGCGCCCAACCACCTGATGAGTGGCTATAGGCACTGGATATATTAGGTGGCGGTGCACTTTCTTACATAAAGGTATTTCCTTTTCTGCGGAAAAGGaa                                                                                                                                       >  1:110473/1‑143 (MQ=255)
      caAAAAGCTAAATTTTCCCCCATCAATAAAAATATAATCCCGCGCCCAACCACCTGATGAGTGGCTATAGGCACTGGATCTATTAGGTGGCGGTGCACTTTATTACATCAAAGTATTTCCTTTTCTGCGGAAAAGGAAATctg                                                                                                                                  >  2:522657/1‑141 (MQ=255)
      caAAAAGCTAAATTTTCCCCCATAAATAAAAATATAATCCCGCGCCCAACCACCTGATGAGTGGCTATAGGCACTGGATATATTAGGTGGCGGTGCACTTTCTTACATAAAGGTATTTCCTTTTCTGCGGAAAAGGaatccgg                                                                                                                                  >  2:221713/1‑138 (MQ=255)
       aaaaaGCTAAATTTTCCCCCATAAATAAAAATATAATCCCGCGCCCAACCACCTGATGAGTGGCTATAGGCACTGGATATATTAGGTGGCGGTGCACTTTCTTACATAAAGGTATTTCCTTTTCTGCGGAAAAGGAAATCggg                                                                                                                                 >  2:83557/1‑143 (MQ=255)
           aGCGAAAGTTGCCCGCATAAATAAAAATATAATCCCGCGCCCAACCACCTGATGAGTGGCTATGCGCACTGGATATATTAGGTGGCGGTGCACTTTCTTACATAAAGGTATTTCCTTTTCTGCGGAAAAGGAAATCGGGaaac                                                                                                                             <  2:722829/143‑2 (MQ=255)
                caTTTTCCCCCATAAATAGAAATATAATCCCGCGCCCAACCCCCTGATGAGTGGCTACAGGCACTGGATATCTTAGGTGGCGGTGCTTGTTCTTACATAAAGGTATTTCCTTTTCTGCGGAAAAGGAAATCGGGAAATCCCCg                                                                                                                        <  2:872176/142‑1 (MQ=255)
                      ccaccgtaaatagaAAGATAGTCCCGCGCCCAGCCGCCTGAGGAGTGGCTATAGGCACTGGATATATTAGGTGGCGGTGCACTTTCTTACATAAAGGTATTTCCTTTTCTGCGGAAAAGGAAATCGGGAAATCCCCGGttttt                                                                                                                  <  2:841586/137‑1 (MQ=255)
                       ccccATAAATAAAAATATAATCCCGAGCCCAACCACCTGATGAGTGGCTATAGGCACTGGATATATTAGGTGGCGGTGCACTTTCTTACATAAAGGTATTTCCTTTTCTGCGGAAAAAGAAAACGGGAAATCACAGGTCTTTc                                                                                                                 >  2:229006/1‑143 (MQ=255)
                         ccATAAATAAAAATATAATCCCGCGCCCAACCCCCTGATGAGTGGCTATAGTCACTGGATATATTAGGTGGCGGTGCACTTTCTTACATAAAGGTATTTCCTTTTCTGCGGAAAAGGAAATCGGGAAATCACCGGTTTTTCTg                                                                                                               >  2:469570/1‑143 (MQ=255)
                          cATAAATAAAAATATAATCCCGCGCCCAACCACCTGATGAGTGGCTATAGGCACTGGATATATTAGGTGGCGGTGCACTTTCTTACATAAAGGTATTTCCTTTTCTGCGGAAAAGGAAATCGGGAAATCCCCGGTTTTTCTGa                                                                                                              <  1:417972/143‑1 (MQ=255)
                                       acaaTCCCGCGCCCTGCCGCCTGAGGAGTGGCTATAGGCACTGGATATATTAGGTGGCGGTGCACTTTCTTACATAAAGGTATTTCCTTTTCTGCGGAAAAGGAAATCGGGAAATCCCCGGTTTTTCTGACAAGCAGACGCCa                                                                                                 <  2:259335/141‑1 (MQ=255)
                                           tCCCGCGCCCAACCACCTGATGAGTGGCTATAGGCACTGGATATATTAGGTGGCGGTGCACTTTCTTACATAAAGGTATTTCCTTTTCTGCGGAAAAGGAAATCGGGAAATCCCCGGTTTTTCTGACAAGCAGACGCCattat                                                                                             >  1:588247/1‑143 (MQ=255)
                                              cgcgCCCAACCACCTGATGAGTGGCTATAGGCACTGGATATATTAGGTGGCGGTGCACTTTCTTACATAAAGGTATTTCCTTTTCAGCGGCACAGGAAATCGGGAAATGCCCAGGTTATGTGACGGGCAGACGCCAATAATtg                                                                                          >  2:260089/1‑143 (MQ=255)
                                               gcgcCCAACCACCTGATGAGTTGCTATAGGCACTGGATATATTAGGTGGCGGTGCACTTTCTTACATAAAGGTATTTCCTTTTCTGCGGAAAAGGAAATCGGGAAATCCCCGGTTTTTCTGACAAGCAGACGCCATTATTtgt                                                                                         <  2:175397/143‑1 (MQ=255)
                                                   ccAACCACCTGATGAGTGGCTATAGGCACTGGATATATTAGGTCGCGGTGCACTTTCTTACATAAAGGTATTTCCTTTTCTGCGGAAAAGGAAATCGGGAAATCCCCGGTTTTTCTGCCACGACGACGCACTTAGTTTTGTCt                                                                                     >  2:488189/1‑143 (MQ=255)
                                                   ccAACAACATGTTGTGTGGCTATAGGCACTGGATATATTAGGTGGCGGTGCACTTTCTTACATAAAGGTATTTCCTTTTCTGCGGAAAAGGAAATCGGGAAATCCCCGGTTTTTCTGACAAGCAGACGCCATTATTTGTGTCt                                                                                     <  1:326944/143‑1 (MQ=255)
                                                    cAACCACCTGATGAGTGGCTATAGGCACTGGATATATTAGGTGGCGGTGCACTTTCTTACATAAAGGTATTTCCTTTTCTGCGGAAAAGGAAATCGGGAAATCCCCGGTTTTTCTGACAGGCATACGCCATGATTTGTGTCTg                                                                                    >  2:579289/1‑143 (MQ=255)
                                                     aaCCGCCTGTTGAGTGTCTATAGGTACTGTATTTATTAGGTGGCGGTGCAGTTTCTTACATAAAGGTATTTCCTTTTCTGCGGAAATGGATATCTGGAAATCCTCGTTTTTTCTGACAAGCAGACGCCATTATTTGTGTCTGc                                                                                   <  2:379027/143‑1 (MQ=255)
                                                            tgatgaGTGGCTATAGGCACTGGATATATTAGGTGGCGGTGCACTTTCTTACATAAAGGTATTTCCTTTTCTGCGGAAAAGGAAATCGGGAAATCCCCGGTTCTTCTGAAAGGGAGACGCCAGAAAGTGGGTCTGCCTATGtt                                                                            >  1:331095/1‑143 (MQ=255)
                                                                gaGTGGCTATAGGCACTGGATATATTAGGTGGCGGTGCACTTTCTTACATAAAGGTATTTCCTTTTCTGCGGAAAAGGAAATCGGGAAATCCCCGGTTTTTCTGACAAGCAGACGCCATTATTTGTGTCTGCCTATgatgggt                                                                        >  2:251513/1‑137 (MQ=255)
                                                                 aGTGGCTATAGGCACTGGATATATTAGGTGGCGGTGCACTTTCTTACATAAAGGTATTTCCTTTTCTGCGGAAAAGGAAATCGGGAAATCCCCGGTTTTTCTGACAAGCAGACGCCATTATTTGTGTCTGCCTATGTTCGTTa                                                                       >  1:698684/1‑143 (MQ=255)
                                                                 aGTGGCTAGAGGCACTGGATATATTAGGTGGCGGTGCACTTTCTTACATAAAGGTATTTCCTTTTCTGCGGAAAAGGAAATCGGGAAATCCCCGGTTTTTCTGACAAGCAGACGCCATTATTTGTGTCTGCCTATGTTCGTTa                                                                       <  1:111638/143‑1 (MQ=255)
                                                                     gCTATAGGCACTGGATATATTAGGTGGCGGTGCACTTTCTTACATAAAGGTATTTCCTTTTCTGCGGAAAAGGAAATCGGGGCATCCCCGGTGTTTCTGACAAGCAGACGCCATTATTTGTGTCTGCCTATGTTCGTTAATTc                                                                   >  2:535056/1‑143 (MQ=255)
                                                                      ctgtgggCACTGGGTATATTAGGTGGCGGTGCACTGTCTTACATAAAGGTATTTCCTTGTCTGCGGAAAAGGAAATCGGGAAATCCCCGGTTTTTCTGACAAGCAGACGCCATTATTTGTGTCTGCCTATGTTCGTTAATTCg                                                                  <  1:396364/138‑1 (MQ=255)
                                                                                  gATATATTAGGTGGCGGTGCACTTTCTTACATAAAGGTATTTCCTTTTCTGCGGAAAAGGAAATCGGGAAATCCCCGGTTTTTCTGACAAGCAGACGCCATTATTTGTGTCTGCCTATGTTCGTTAATTCGTTCATCAGGaaa                                                      >  2:18324/1‑143 (MQ=255)
                                                                                  gATATATTAGGTGGCGGTGCACTTTCTTACATAAAGGTATTTCCTTTTCTGCGGAAAAGGAAATCGGGAAATCCCCGGTTTTTCTGACAAGCAGACGCCATTATTTGTGTCTGCCTATGTTCGTTAATTCGTTCATCAGGaaa                                                      >  2:668490/1‑143 (MQ=255)
                                                                                    tatatTAGGTGGCGGTGCACTTTCTTACATAAAGGTATTTCCTTTTCTGCGGAAAAGGAAATCGGGAAATCCCCGGTTTTTCTGACAAGCAGACGCCATTATTTGTGTCTGCCTATGTTCGTTAATTCGTTCATCAGGAAAtt                                                    >  1:222299/1‑143 (MQ=255)
                                                                                     atacTATGTGGCGGTGCACTTTCGTACATAAAGGTATTCCCTTTTCTGCGGAAAAGGAAATCGGGAAATCCCCGGTTTGTCTGACAAGCAGACGCCATTATTTGTGTCTGCCTATGTTCGTTAATTCGTTCATCAGGAAATTa                                                   <  1:358663/143‑1 (MQ=255)
                                                                                         aaGGTGGCGGTGCACCTCCTTACATAACGGTATTTCCCTTTCTGCGGAAAAGGAAATCGGGAAATCCCCGGTTTTCCTGACAAGCAGACGCCATTATTTGTGTCTGCCTATGTTCGTTAATTCGTTCATCAGGAAATTAtctc                                               <  1:34818/142‑1 (MQ=255)
                                                                                           ggTGGCGGTGCACTTTCTTACATAAAGGTATTTCCTTTTCTGCGGAAAAGGAAATCGGGAAATCCCCGGTTTTTCTGACAAGCAGACGCCATTATTTGTGTCTGCCTATGTTCGTTAATTCGCTCATCAGGAACTTATCTCaa                                             >  1:295810/1‑143 (MQ=255)
                                                                                                cGGTGCACTTTCTTACATAAAGGTATTTCCTTTTCTGCGGAAAAGGAAATCGGGAAATCCCCGGTTTTTCTGACAAGCAGACGCCATTATTTGTGTCTGCCTATGTTCGTTAATTCGTTCATCAGGAAATTATCTCAATGTca                                        <  1:16453/143‑1 (MQ=255)
                                                                                                   tGCACTTTCTTACATAAAGGTATTTCCTTTTCTGCGGAAAAGGAAATCGGGAAATCCCCGGTTTTTCTGACAAGCAGACGCCATTATTTGTGTCTGCCTATGTTCGTTAATTCGTTCATCAGGAAATTATCTCAATGTCACAt                                     <  2:110473/143‑1 (MQ=255)
                                                                                                   tGCACTTTCTTACATAAAGGTATTTCCTTTTCTGCGGAAAAGGAAATCGGGAAATCCCCGGTTTTTCTGACAAGCAGACGCCATTATTTGTGTCTGCATATGTTCGTTAATTCGTTCATCAGGAAATTATCTCAATGTCACAt                                     >  2:810499/1‑143 (MQ=255)
                                                                                                    gCACTTTCTTACATAAAGGTATTTCCTTTTCTGCGGAAAAGGAAATCGGGAAATCCCCGGTTTTTCTGACAAGCAGACGCCATTATTTGTGTCTGCCTATGTTCGTTAATTCGTTCATCAGGAAATTATCTCAATGTCACAtt                                    >  1:404185/1‑143 (MQ=255)
                                                                                                        tttCTTACATAAAGGTATTTCCTTTTCTGCGGAAAAGGCAATCGGGAAATCCCCGGTTTTTCTGACAAGCAGACGCCATTATTTGTGTCTGACTATGTTCGTTAATTCGTTCATCAGTAAATTATCTCAATGTCACATtatta                                >  2:226750/1‑141 (MQ=255)
                                                                                                             tACATAAAGGTATTTCCTTTTCTGCGGAAAAGGAAATCGGGAAATCCCCGGTTTTTCTGACAAGCAGACGCCATTATTTGTGTCTGCCTATGTTCGTTAATTCGTTCATCAGGAAATTATCTCAATGTCACATTATAAACCAg                           >  2:290665/1‑143 (MQ=255)
                                                                                                                 tAAAGGTATTTCCTTTTCTGCGGAAAAGGAAATCGGGAAAGCCCCGGTTTTTCTGACAAGCAGACGCCATTATTTGTGTCTGCCTATGTTCGTTAATTCGTTCATCAGGAAATTATCTCAATGTCACATTATAAAACAGGTCa                       <  1:221713/143‑1 (MQ=255)
                                                                                                                  aaaGGTATTTCCTTTTCTGCGGAAAAGGAAATCGGGAAATCCCCGGTTTTTCTGACAAGCAGACGCCATTATTTGTGTCTGCCTATGTTCGTTAATTCGTTCATCAGGAAATTATCTCAATGTCACATTATAAAACAGGTCAt                      <  1:668490/143‑1 (MQ=255)
                                                                                                                   aaGGTATTTCCTTTTCTGCGGAAAAGGAAATCGGGAAATCCCCGGTTTTTCTGACAAGCAGACGCCATTATTTGTGTCTGCCTATGTTCGTTAATTCGTTCATCAGGAAATTATCTCAATGTCACATTATAAAACAGGTCATa                     <  1:83557/143‑1 (MQ=255)
                                                                                                                         tttCCTTTTCTGCGGAAAAGGAAATCGGGAAATCCCCGGTTTTTCTGACAAGCAGAGGCCATTATTTGTGTCTGCCTATGTTCGTTAATTCGTTCATCAGGAAATTATCTCAATGTCACATTATAAAACAGGTCATAaacaac               >  2:881488/1‑143 (MQ=255)
                                                                                                                         tttCCTTTTCCGCAGAAAAGGAAATCGGGGAATGACCGGTTTTTCTGACAAGCAGACGCCATTATTTGTGTCTGCCTATGTTCGTTAATTCGTTCATCAGGAAATTATCTCAATGTCACATTATAAAACAGGTCATGaacaac               >  1:804844/1‑143 (MQ=255)
                                                                                                                         tttCCTTTTCCGCAGAAAAGGAAATCGGGAAATCCCCGGTTTTTCTGACAAGCAGACGCCATTATTTGTGTCTGCCTATGTTCGTTAATTCGTTCATCAGGAAATTATCTCAATGTCACATTATAAAACAGGTCATAaacaac               >  1:749136/1‑143 (MQ=255)
                                                                                                                           tCCTTTTCCGCAGAAAAGGAAATCGGGAAATCCCCGGTTTTTCTGACAAGCAGACGCCATTATTTGTGTCTGCCTATGTTCGTTAATTCGTTCATCAGGAAATTATCTCAATGTCACATTATAAAACAGGTCATAAACAACCa             >  2:593507/1‑143 (MQ=255)
                                                                                                                              ttttCTGCGGAAAAGGAAATCGGGAAATCCCCGGTTTTTCTGACAAGCAGACGCCATTATTTGTGTCTGCCTATGTTCGTTAATTCGTTCATCAGGAAATTATCTCAATGTCACATTATAAAACAGGTCATAAACAACCACGa          >  2:731207/1‑143 (MQ=255)
                                                                                                                                   ggCGGAAAAGGAAATCGGGAAATCCCCGGTGTTTCTGACAAGCAGAGGCCATTATTTGTGTCTGCCTATGTTCGTTAATTCGTTCATCAGGAAATTATCTCAATGTCACATTATAAAACAGGTCATAAACAACCACGATTTCg     <  2:325401/142‑1 (MQ=255)
                                                                                                                                     cGGAAAAGGAAATCGGGAAATCCCCGGTTTTTCTGACAAGCAGACGCCATTATTTGTGTCTGCCTATGTTCGTTAATTCGTTCATCAGGAAATTATCTCAATGTCACATTATAAAACAGGTCATAAACAACCACGATTTCGtt   >  1:483104/1‑143 (MQ=255)
                                                                                                                                     cGGAAAAGGAAATCGGGAAATCCCCGGTTTTTCTGACAAGCAGACGCCATTATTTGTGTCTGCCTATGTTCGTTAATTCGTTCATCAGGAAATTATCTCAATGTCACATTATAAAACAGGTCATAAACAACCACGATTTCGtt   >  1:479931/1‑143 (MQ=255)
                                                                                                                                      ggAAAAGGAAATCGTGAAATCCCCTGTTTTTCTGACAAGCAGACGCCATTATTTGTGTCTGCCTATGTTCGTTAATTCGTTCATCAGGAAATTATCTCAATGTCACATTATAAAACAGGTCATAAACAACCACGATTTCGTTa  <  2:819679/143‑1 (MQ=255)
                                                                                                                                      |                                                                                                                                              
CTTGCACAAAAAGCTAAATTTTCCCCCATAAATAAAAATATAATCCCGCGCCCAACCACCTGATGAGTGGCTATAGGCACTGGATATATTAGGTGGCGGTGCACTTTCTTACATAAAGGTATTTCCTTTTCTGCGGAAAAGGAAATCGGGAAATCCCCGGTTTTTCTGACAAGCAGACGCCATTATTTGTGTCTGCCTATGTTCGTTAATTCGTTCATCAGGAAATTATCTCAATGTCACATTATAAAACAGGTCATAAACAACCACGATTTCGTTA  >  NC_000913/314124‑314400

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑