Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 493,634 G→A 8.0% A515T (GCA→ACA)  dnaX → DNA polymerase III subunit tau

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913493,6340GA 8.0% 132.2 / 3.1 50A515T (GCA→ACA) dnaXDNA polymerase III subunit tau
Reads supporting (aligned to +/- strand):  ref base G (21/25);  new base A (2/2);  total (23/27)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.06e-01

GCGCCAGCCAAAAAAGAAGCGTATCGCTGGAAGGCGACCACTCCGGTGATGCAGCAAAAAGAAGTGGTCGCCACGCCGAAGGCGCTGAAAAAAGCGCTGGAACATGAAAAAACGCCGGAACTGGCGGCGAAGCTAGCGGCAGAAGCCATTGAGCGCGACCCGTGGGCGGCACAGGTGAGCCAACTTTCGCTACCAAAACTGGTCGAACAGGTGGCGTTAAATGCCTGGAAAGAGGAGAGCGACAACGCAGTATGTCTGCATTTGCGCTCCTCTCAGCGGCA  >  NC_000913/493496‑493776
                                                                                                                                          |                                                                                                                                              
gcgcCAGCCAAAAAAGAAGCGTATCGCTGGAAGGCGACCACTCCGGTGATGCAGCAAAAAGAAGTGGTCGCCACGCCGTAGGCGCTGAAAAAAGCGCTGGAACATGAAAAAACGCCGGAACTGGCGGCGAAGCTAGCGGCAGa                                                                                                                                            >  2:719132/1‑143 (MQ=255)
 cgcCAGCCAAAAAAGAAGCGTAACGCTGGAAGGCGACCACTCCGGTGATGTGGGTAGAACAACTCGTTGCGACTCCGAAGGCGCTGAAAAAAGCGCTGGAACATGAAAAAACGCCGGAACTGGCGGCGAAGCTAGCGGCAGaa                                                                                                                                           <  2:632886/143‑1 (MQ=255)
   ccagccaAAAAAGAAGCGTATCGCTGGAAGGCGACCACTCCGGTGATGCAGCAAAAAGAAGTGGTCGCCACGCCGAAGGCGCTGAAAACAGCGCTGGAACATGAAAAAACGCCGGAACTGGCGGCGAAGCTAGCGGCAGAAGc                                                                                                                                         >  2:102327/1‑143 (MQ=255)
     agccaAAAAAGAAGCGTATCGCTGGAAGGCGACCACTCCGGTGATGCAGCAAAAAGAAGTGGTCGCCACGCCGAAGGCGCTGAAAAAAGCGCTGGAACATGAAAAAACGCCGGAACTGGCGGCGAAGCTAGCGGCAGAAGCCa                                                                                                                                       <  1:856311/143‑1 (MQ=255)
         aaaaaaGAAGCGTATCGCTGGAAGGCGACCACTCCGGTGATGCAGCAAAAAGAAGTGGTCGCCACGCCGCAGGCGCCGAAAAAAGCGCTGGAACATGACAACACGCCGGAACTGGCGGCGAAGCTAGCGGCAGAAGCCAtcca                                                                                                                                   >  2:207265/1‑140 (MQ=255)
         aaaaaaGAAGCGGATGGCGGGAAGGAGACCACTCAGGGGATGCAGCAAAAAGAAGTGGTCGCCACGCCGAAGGCGCTGAAAAAAGCGCTGGAACATGAAAAAACGCCGGAACTGGCGGCGAAGCTAGCGGCAGAAGCCATTGa                                                                                                                                   <  1:340176/143‑1 (MQ=255)
                     tATCGCTGGAAGGCGACCACTCCGGTGATGCAGCAAAAAGAAGTGGTCGCCACGCCGAAGGCGCTGAAAAAAGCGCTGGAACATGAAAAAACGCCGGAACTGGCGGCGAAGCTAGCGGCAGAAGCCATTGAGCGCGACCCGTg                                                                                                                       >  1:149862/1‑143 (MQ=255)
                         gCTGGAAGGCGACCACTCCGGTGATGCAGCAAAAAGAAGTGGTCGCCACGCCGAAGGCGCTGAAAAAAGCGCTGGAACATGAAAAAACGCCGGAACTGGCGGCGAAGCTAGCGGCAGAAGCCATTGAGCGCGACCCGTGggcg                                                                                                                   >  2:737722/1‑143 (MQ=255)
                          cggcaaagCGACCACTCGGGTGATGCAGCAAAAAGAATTGGTCGCCACGCCGAAGGCGCTTAAAAAAGCTCTGGAACATGAAAAAACGCCGGAACTGGCGGCGAAGCTAGCGGCAGAAGCCATTGAGCGCGACCCGTGggcgg                                                                                                                  <  2:75098/136‑1 (MQ=255)
                                ggCGACCACTCCGGTGATGCAGCAAAAAGAAGTGGTCGCCACGCCGAAGGCGCTGAAAAAAGCGCTGGAACATGAAAAAACGCCGGAACTGGCGGCGAAGCTAGCGGCAGAAGCCATTGAGCGCGACCCGTGGGCGGCACAgg                                                                                                            >  2:163873/1‑143 (MQ=255)
                                 gCGACCACTCCGGTGATGCAGCAAAAAGAAGTGGTCGCCACGCCGAAGGCGCTGAAAAAAGCGCTGGAACATGAAAAAACGCCGGAACTGGCGGCGAAGCTAGCGGCAGAAGCCATTGAGCGCGACCCGTGGGCGGCACAGGt                                                                                                           <  1:239652/143‑1 (MQ=255)
                                   gACCACTCCGGTGATGCAGCAAAAAGAAGTGGTCGCCACGCCGAAGGCGCTGAAAAAAGCGCTGGAACATGACCAGACGCCGCAACTGGCGGCGAAGCTAGCGGCAGAAGCCATTGAGCGCGACCCGTGGGCGGCACAGGTGa                                                                                                         <  1:191420/143‑1 (MQ=255)
                                   gACCACTCCGGGGATGCAGCAAAAAGAAGTGGTCGCCACGCCGAAGGCGCTGAAAAAAGCGCTGGAACATGAAAAAACGCCGGAACTGGCGGCGAAGCTAGCGGCAGAAGCCATTGAGCGCGACCCGTGGGCGGCACAGGTGa                                                                                                         <  2:792727/143‑1 (MQ=255)
                                    aCCACTCCGGTGATGCAGCAAAAAGAAGTGGTCGCCACGCCGAAGGCGCTGAAAAAAGCGCTGGAACATGAAAAAACGCCGGAACTGGCGGCGAAGCTAGCGACAGAAGCCATTGAGCGCGACCCGTGGGCGGCACAGGTGAg                                                                                                        >  1:875161/1‑143 (MQ=255)
                                    aCCACTCCGGTGATGCAGCAAAAAGAAGTGGTCGCCACGCCGAAGGCGCTGAAAAAAGCGCTGGAACATGAAAAAACGCCGGAACTGGCGGCGAAGCTAGCGACAGAAGCCATTGAGCGCGACCCGTGGGCGGCACAGGTGAg                                                                                                        >  1:875080/1‑143 (MQ=255)
                                         tCCGGTGATGCAGCAAAAAGAAGTGGTCGCCACGCCGAAGGCGCTGAAAAAAGCGCTGGAACATGAAAAAACGCCGGAACTGGCGGCGAAGCTAGCGGCAGAAGCCATTGAGCGCGACCCGTGGGCGGCACAGGTGAGCCAAc                                                                                                   >  1:717125/1‑143 (MQ=255)
                                         tCCGGTGATGCAGCAAAAAGAAGTGGTCGCCACGCCGAAGGCGCTGAAAAAAGCGCTGGAACATGAAAAAACGCCGGAACTGGCGGCGAAGCTAGCGGCAGAAGCCATTGAGCGCGACCCGTGGGCGGCACAGGTAAGCCAAc                                                                                                   >  2:687222/1‑143 (MQ=255)
                                               gATGCAGCAAAGAGAAGTGGTCGCCACGCCGAAGGGGGTGAAAAAAGCGCGGGAACATGAAAAAACGCCGGAACTGGCGGCGAAGCTAGCGGCAGAAGCCATTGAGCGCGACCCGTGGGCGGCACAGGTGAGCCAACTTTCGc                                                                                             <  2:161496/143‑1 (MQ=255)
                                                  gcagcaAAAAGAAGTGGTCGCCACGCCGAGGGCGCGTAAAAAAGCGCTGGAACATGAAAAAACGCCGGAACTGGCGGCGAAGCTAGCGGCAGAAGCCATTGAGCGCGACCCGTGGGCGGCACAGGTGAGCCAACTTTCGCTAc                                                                                          <  1:605298/143‑1 (MQ=255)
                                                   cagcaAAAAGAAGGGGTCGACACGCCAAGGGCGCTGAAAAAAGCGCTGGAACATGAAGAAACGCCGGAACTGGCGGCGAAGCTAGCGGCAGAAGCCATTGAGCGCGACCCGTGGGCGGCACAGGTGAGCCAACTTTCGCTAcc                                                                                         <  1:62049/143‑1 (MQ=255)
                                                        aaaaGAAGTGGTCGCCACGCCGAAGGCGCTGAAAAAAGCGCTGGAACATGAAAAAACGCCGGAACTGGCGGCGAAGCTAGCGGCAGAAGCCATTGAGCGCGACCCGTGGGCGGCACGGGTGAGCCAACTTTCGCTACCAAAAc                                                                                    >  1:259520/1‑143 (MQ=255)
                                                          aagaagTGGTCGCGACGCCGAAGGCGCTGAAAAAAGCGCTGGAACATGAAAAAACGCCGGAACTGGCGGCGAAGCTAGCGGCAGAAGCCATTGAGCGCGACCCGTGGGCGGCACAGGTGAGCCAACTTTCGCTACCAAAACTg                                                                                  <  2:202343/143‑1 (MQ=255)
                                                          aagaagTGGTCGCCACGCCGAAGGCGCTGAAAAAAGCGCTGGAACATGAAAAAACGCCGGAACTGGCGGCGAAGCTAGCGGCAGAAGCCATTGAGCGCGACCCGTGGGCGGCACAGGTGAGCCAACTTTCGCTACCAAAACTg                                                                                  <  1:737722/143‑1 (MQ=255)
                                                             aagTGGTCGCCACGCCGAAGGCGCTGAAAAAAGCGCTGGAACATGAAAAAACGCCGGAACTGGCGGCGAAGCTAGCGGCAGAAGCCATTGAGCGCGACCCGTGGGCGGCACAGGTGAGCCAACTTTCGCTACCAAAACTGGTc                                                                               <  1:352399/143‑1 (MQ=255)
                                                             aagTGGTCGCCACGCCGAAGGCGCTGAAAAAAGCGCTGGAACACGAAAACACGCCGGAACTGGCGGCGAAGCTAGCGGCAGAAGCCATTGAGCGCGACCCGTGGGCGGCACAGGTGAGCCAACTTTCGCTACCAAAACTGGTc                                                                               <  2:878340/143‑1 (MQ=255)
                                                               gTGGTCGCCACGCCGAAGGCGCTGAAAAAAGCGCTGGAACATGAAAAAACGCCGGAACTGGCGGCGAAGCTAGCGGCAGAAGCCATTGAGCGCGACCCGTGGGCGGCACAGGTGAGCCAACTTTCGCTACCAAAACTGGTCGa                                                                             >  1:756022/1‑143 (MQ=255)
                                                                tGGTCGCCACGCCGAAGGCGCTGAAAAAAGCGCTGGAACATGAAAAAACGCCGGAACTGGCGGCGAAGCTAGCGGCAGAAGCCATTGAGCGCGACCCGTGGGCGGCACAGGTGAGCCAACTTTCGCTACCAAAACTGGTCGaa                                                                            >  1:740465/1‑143 (MQ=255)
                                                                     gCCACGCCGAAGGCGCTGAAAAAAGCGCTGGAACATGAAAAAACGCCGGAACTGGCGGCGAAGCTAGCGGCAGAAACCATTGAGCGCGACCCGTGGGCGGCACAGCTGAGCCAACTTTCGCTACCCAAACTGGTCGAAccgct                                                                       >  2:686878/1‑139 (MQ=255)
                                                                      ccACGCCGAAGGCGCTGAAAAAAGCGCTGGAACAGGAAAAAACGCCGGAACTGGCGGCGAAGCTAGCGGCAGAAGCCATTGAGCGCGACCCGTGGGCGGCACAGGTGAGCGAACTTGCGCTACCAAAACTCGTCGCACCGGtc                                                                      >  2:414573/1‑142 (MQ=255)
                                                                       cACGCCGAAGGCGCTGAAAAAAGCGCTGGAACATGAAAAAACGCCGGAACTGGCGGCGAAGCTAGCGGCAGAAGCCATTGAGCGCGACCCGTGGGCGGCACAGGTGAGCCAACTTTCGGTACCAAAACTGGTCGAACAGGTgg                                                                     >  1:753402/1‑143 (MQ=255)
                                                                       cACGCCGAAGGCGCTGAAAAAAGCGCTGGAACATGAAAAAACGCCGGAACTGGCGGCGAAGCTAGCGGCAGAAGCCATAGAGCGCGACCCGAGGGCGGCACAGGTGAGCCAACGTTCGCTACCACCACTCGTCGAACAGGTgc                                                                     >  2:858208/1‑142 (MQ=255)
                                                                              aaGGCGCTGAGAAAAGCGCGGGAACATGAAAAAACGCCGGAACTGGCGGCGAAGCTAGCGGCAGAAGCCATTGCGCGTGACCCGTGGGCGGCACAGGTGAGCCAACTTTCGCTACCAAAACTGGTCGAACAGGTGGCGTTaaa                                                              <  1:827129/143‑1 (MQ=255)
                                                                               aGGCGCTGAAAAAAGCGCTGGAACAGGAAAAAACGCCGGAACTGGCGGCGAAGCTAGCGGCAGAAGCCATTGAGCGCGACCCGTGGGCGGCACAGGTGAGCCAACTTTCGCTACCAAAACTGGTCGAACAGGTGGCGTTAAAt                                                             <  1:119360/143‑1 (MQ=255)
                                                                                 gcgcTGAAAAAAGCGCTGGAACATGAAAAAACGCCGGAACTGGCGGCGAAGCTAGCGGCAGAAGCCATTGAGCGCGACCCGTGGGCGGCACAGGTGAGCCAACTTTCGCTACCAAAACTGGTCGAACAGGTGGCGTTAAATGc                                                           <  1:48813/143‑1 (MQ=255)
                                                                                   gcTGAAAAAAGCGCTGGAACATGAAAACACGCCGGAACTGGCGGCGAAGCTAGCGGCAGAAGCCATTGAGCGCGACCCGTGGGCGGCACAGGTGAGCCAACTTTCGCTACCAAAACTGGTCGAACAGGTGGCGTTAAATGCCt                                                         <  1:714550/143‑1 (MQ=255)
                                                                                       caaaaaGCGCTGGAACATGAAAAAACGCCGGAACTGGCGGCGAAGCTAGCGGCAGAAGCCATTGAGCGCGACCCGTGGGCGGCACAGGTGAGCCAACTTTCGCTACCAAAACTGGTCGAACAGGTGGCGTTAAATGCCTGGaa                                                     <  1:425269/142‑1 (MQ=255)
                                                                                           aggcgcGGGACCATGAAAAACCGCCGGAACTGGCGGCGCAGCTAGCGGCAGAAGCCATTGAGCGCGACCCGTGGGCGGCACAGGTGAGCCAACTTTCGCTACCAAAACTGGTCGAACAGGTGGCGTTAAATGCCTGGAAagag                                                 <  2:305322/141‑1 (MQ=255)
                                                                                               gcTGGAACATGAAAAAACGCCGGAACTGGCGGCGAAGCTAGCGACAGAAGCCATTGAGCGCGACCCGTGGGCGGCACAGGTGAGCCAACTTTCGCTACCAAAACTGGTCGAACAGGTGGCGTTAAATGCCTGGAAAGAGgaga                                             <  2:875161/143‑1 (MQ=255)
                                                                                               gcTGGAACATGAAAAAACGCCGGAACTGGCGGCGAAGCTAGCGACAGAAGCCATTGAGCGCGACCCGTGGGCGGCACAGGTGAGCCAACTTTCGCTACAAAAACTGGTCGAACAGGTGGCGTTAAATGCCTGGAAAGAGgaga                                             <  2:875080/143‑1 (MQ=255)
                                                                                                cTGGAACATGAAAAAACGCCGGAACTGGCGGCGAAGCTAGCGGCAGAAGCCATTGAGCGCGACCCGTGGGCGGCACAGGTGAGCCAACTTTCGCTACCAAAACTGGTCGTACAGGTGGCGTTAAATGCCTGGAAAGAGGagag                                            >  1:294920/1‑143 (MQ=255)
                                                                                                 tGGAACATGAAAAAACGCCGGAACTGGCGGCGAAGCTAGCGGCAGAAGCCATTGAGCGCGACCCGTGGGCGGCACAGGTGAGCCAACTTTCGCTACCAAAACTGGTCGAACAGGTGGCGTTAAATGCCTGGAAAGAGGAGAGc                                           <  1:624644/143‑1 (MQ=255)
                                                                                                  ggAACATGAAAAAACGCCGGAACTGGCGGCGAAGCTAGCGGCAGAAGCCATTGAGCGCGACCCGTGGGCGGCACAGGTGAGCCAACTTTCGCTACCAAAACTGGTCGAACAGGTGGCGTTAAATGCCTGGAAAGAGGAGAGCg                                          <  1:580344/143‑1 (MQ=255)
                                                                                                      cATGAAAAAGCGCCGGAACTGGCGGCGAAGCTAGCGGCAGAAGCCAGTGAGCGCGACCCGTGGGCGGCACAGGTGAGCCAACTTTCGCTACCAAAACTGGTCGAACAGGTGGCGTTAAATGCCTGGAAAGAGGAGAGCGACaa                                      <  1:885437/143‑1 (MQ=255)
                                                                                                              aaCGCCGGAACTGGCGGCGAAGCTAGCGGCAGAAGCCATTGAGCGCGACCCGTGGGCGGCACAGGTGAGCCCACTTTCGCTACCAAAACTGGTCGAACAGGTGGCGTTAAATGCCTGGAAAGAGGAGAGCGACAACGCAGTac                              >  2:179691/1‑142 (MQ=255)
                                                                                                                cGCCGGAACTGGCGGCGAAGCTAGCGGCAGAAGCCATTGAGCGCGACCCGTGGGCGGCACAGGTGAGCCAACTTTCGCTACCAAAACTGGTCGAACAGGTGGCGTTAAATGCCTGGAAAGAGGAGAGCGACAACGCAGTATGt                            <  2:149862/143‑1 (MQ=255)
                                                                                                                 gCCGGAACTGGCGGCGAAGCTAGCGGCAGAAGCCATTGAGCGCGACCCGTGGGCGGCCCAGGGGGGCCAACTCGCGCTACCAACACTGGCCGAACAGGTGGCGTTAAATGCCTGGGAAGAGGAGAGCGCCAACGCAGTATGTc                           >  2:484210/1‑143 (MQ=255)
                                                                                                                         tGGCGGCGAAGCTAGCGGCAGAAGCCATTGAGCGCGACCCGTGGGCGGCACAGGTGAGCCAACTTTCGCTACCACAATTGGTCGACCAGGTGGCGTTAAATGCCTGGAAAGAGGAGAGCGACAGCGCCGTCTGTCTGcctgcg                   >  1:59580/1‑138 (MQ=255)
                                                                                                                                gAAGCTAGCGGCAGAAGCCATTGAGCGCGACCCGTGGGCGGCACAGGTGAGCCAACTTTCGCTACCAAAACTGGTCGAACAGGTGGCGTTAAATGCCTGGAAAGAGGAGAGCGACAACGCAGTATGTCTGCATTAGCGctcct            >  2:649932/1‑143 (MQ=255)
                                                                                                                                    cTAGCGGCAGGAGCCATTGAGCGCGACCCGTGGGCGGCACAGGTGAGCCAACTTTCGCTACCAAAACTGGTCGAACAGGTGGCGTTAAATGCCGGGAAGGAGGAGAGCGATAACGCAGTATGTCTGCATTTGCGCTCCTCTCa        <  1:169400/143‑1 (MQ=255)
                                                                                                                                     tAGCGGCAGAAGCCATTGAGCGCGACCCGTGGGCGGCACAGGTGAGCCAACTTTCGCCACCAAAACTGGTCGAACAGGTGGCGTTAAATGGCGGGACAGCAGAGAGAGACAACGCAGTAGGTCTGCATTTGCGATCCTCGCAg       >  2:264086/1‑143 (MQ=255)
                                                                                                                                          gCAGACGCCATTGAGCGCGACCCGGGGGCGGCACAGGTGAGCCAACTTTCGCTACCAAAACTGGTCGAACAGGTGGCGTTAAATGCCTGGAAAGAGGAGAGCGACAACGCAGTATGTCTGCATTTGCGCTCCTCTCAGCGGCa  <  2:692680/143‑1 (MQ=255)
                                                                                                                                          |                                                                                                                                              
GCGCCAGCCAAAAAAGAAGCGTATCGCTGGAAGGCGACCACTCCGGTGATGCAGCAAAAAGAAGTGGTCGCCACGCCGAAGGCGCTGAAAAAAGCGCTGGAACATGAAAAAACGCCGGAACTGGCGGCGAAGCTAGCGGCAGAAGCCATTGAGCGCGACCCGTGGGCGGCACAGGTGAGCCAACTTTCGCTACCAAAACTGGTCGAACAGGTGGCGTTAAATGCCTGGAAAGAGGAGAGCGACAACGCAGTATGTCTGCATTTGCGCTCCTCTCAGCGGCA  >  NC_000913/493496‑493776

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑