Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 699,556 C→T 7.6% intergenic (‑379/+18) asnB ← / ← umpH asparagine synthetase B/UMP phosphatase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913699,5560CT 7.6% 138.1 / 3.3 53intergenic (‑379/+18)asnB/umpHasparagine synthetase B/UMP phosphatase
Reads supporting (aligned to +/- strand):  ref base C (24/25);  new base T (2/2);  total (26/27)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.69e-01

ATTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACAAAAGCATGAAAATTCAATATATTGCAGGAGCTGCGTAGGCCTGATAAGCGTAGCGCATCAGGCAGTTTGGCGTTTGTCATCAGAGCCAACCACGTCCGCAGACGTGGTTGCTATTCAGATAACGTCGATTTCAGCGACTGACGGGTAAATCCAGCTGGGGCGGAAAGGCATACTGTCGATATCGTCGAGCGACGAAACACCAGAAAGCACCAGAATCGTCTCCAGACCTGCCTGGAAG  >  NC_000913/699415‑699697
                                                                                                                                             |                                                                                                                                             
aTTTGGTGATGCCGGGGGCGGCGGGGACGCCTTATCCGGCCTACAAGAGCATGAAAATTCAATATATTGCAGGAGCTGCGTAGGCCTGATAAGCGTAGCGCATCAGGCAGTTTGGCGTTTGTCATCAGAGCCAACCACGTCCg                                                                                                                                              <  1:526218/143‑1 (MQ=21)
 tttGTTCATGCCGGATGCGGCGTGAAGGCCTTAGCCGGCCTACAAAAGCATGAGAATTCAAGAGAGTGAAGGAGCGGCGTCGGCCTGATAAGCGTAGCGCATCAGGCAGTTTGGCGTTTGTCATCAGAGCCAACCACGTCCGc                                                                                                                                             <  1:441568/143‑1 (MQ=18)
      tCATGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACAAAAGCATGAAAATTCAATATATTGCAGGAGCTGCGTAGGCCTGATAAGCGTAGCGCATCAGGCAGTTTGGCGTTTGTCATCAGAGCCAACCACGTCCGCAGACg                                                                                                                                        <  1:492511/143‑1 (MQ=255)
          gCCGCATGCGGCGTGAACGCCTTATCCGGCCTACAAAAGCATGAAAATTCAATATATTGCAGGAGCTGCGTAGGCCTGATAAGCGTAGCGCATCAGGCAGTTTGGCGTTTGTCATCAGAGCCAACCACGTCTGCGGACGTGGt                                                                                                                                    <  1:472271/143‑1 (MQ=255)
            tggATGCGTTGTTAACGCCTTATCCGGCCTACAAAAGCATGAAAATTCAATATATTGCAGGAGCTGCGTAGGCCTGATAAGCGTAGCGCATCAGGCAGTTTGGCGTTTGTCATCAGAGCCAACCACGTCTGCGGACGTGGTTg                                                                                                                                  <  1:56079/142‑1 (MQ=21)
            cGGATGCGGGGTGAACGCCGTATCCGGACTACAAAAGCGTGAAAATTCAAGATGTTGCAGGGGCTGCGTAGGCGTGATAAGCGTAGCGCAGCAGGCAGGTGGGCGTTTGTCATCAGAGCCAACCACGTCCGCAGACGTGGTTg                                                                                                                                  <  2:775660/143‑1 (MQ=255)
              gacgcggcgTGAACGCCTTATCCGGCCTACAAAAGCATGACAATTCAATATATTGCAGGAGCTGCGTACGCCTGATAAGCGTAGCGCATCAGGCAGTTTGGCGTTTGTCATCAGAGCCAACCACGTCCGCAGACGTGGTTGCt                                                                                                                                <  1:133756/140‑1 (MQ=255)
              gATGCGGCGTGAACGCCTTATCCGGCCTACAAAAGCATGAAAATTCAATATATTGCAGGAGCTGCGTAGGCCTGATAAGCGTAGCGCATCAGGCAGTTTGGCCTTTGTCATCAGAGCCAACCACGTCCCAAGACGTGGTTGCt                                                                                                                                >  2:111875/1‑143 (MQ=255)
                 gcggcgTGAACGCCTTATCCGGCCTACAAAAGCATGAAAATTACGGGGGGTTCATGAGCTGCGTAGGCCTGATAAGCGTAGCGCATCAGGAAGTTTGGCGTTTGTCATCAGAGCCAACCACGTCCGCAGAAGTGGGTGCTAtt                                                                                                                             >  2:191079/1‑143 (MQ=21)
                    gcgTGAACGCCTTATCCGGCCTACAAAAGCATGAAAATTCAATATATTGCAGGAGCTGCGTAGGCCTGATAAGCGTAGCGCATCAGGCAGTTTGGCGTTTGTCATCAGAGCCAACCACGTCCGCAGACGTGGTTGCTATTCAg                                                                                                                          >  1:40299/1‑143 (MQ=255)
                    gcgTGAACGCCTTATCCGGCCTACAAAAGCATAAAAATTCAATATATTGCAGGAGCTGCGTAGGCCTGATAAGCGTAGCGCATCAGGCAGTTTGGCGTTTGTCATCAGAGCCAACCACGTCCGCAGACGTGGTTGCTATTCAg                                                                                                                          >  2:191990/1‑143 (MQ=255)
                      tggAACGCCTTATCCGGCCTACAAAGGCATGAAAATTCAATATATTGCAGGAGCTGCGTAGGCCTGATAAGCGTAGCGCATCAGGCAGTTTGGCGTTTGTCATCAGAGCCAACCACGTCCGCAGACGTGGTTGCTATTCAGAt                                                                                                                        <  2:456093/141‑1 (MQ=255)
                                tATCCGGCCTACAAAAGCATGAAAATTCAATATATTGCAGGAGCTGCGTAGGCCTGATAAGCGTAGCGCATCAGGCAGTTTGGCGTTTGTCATCAGAGCCAACCACGTCCGCAGACGTGGTTGCTATTCAGATAACGTCGAtt                                                                                                              >  2:881442/1‑143 (MQ=255)
                                 aTCCGGCCTACAAAAGCATGAAAATTCAATATATTGCAGGAGCTGCGTAGGCCTGATAAGCGTAGCGCATCAGGCAGTTTGGCGTTTGTCATCAGAGCCAACCACGTCCGCAGCCGGGGGTGTTATTAACAGAACGTCGAttt                                                                                                             >  2:530314/1‑143 (MQ=25)
                                                   tGAAAATTCAATATATTGCAGGAGCTGCGTCAGCAAATGTTTCGTATCGCATCAGGCAGTTTGGCGTTTGTCATCAGAGCCAACCACGTCCGCAGACGTGGTTGCTATTCAGATAACGTCGATTTCAGCGACTGACGGGTaaa                                                                                           <  2:866335/143‑1 (MQ=255)
                                                   tGAAAATTCAATATATTGCAGGAGCTGCGTAGGCCTGATAAGCGTAGGGCATCATCGAGTTTGGCGTTTATCATCAGAGCCAACCACGTCCGCAGACGTGGTTGCTATTCAGATAACGTCGATTTCAGCGACTGACGGGTaaa                                                                                           <  2:58393/143‑1 (MQ=38)
                                                    gAAAATTCAATATATTGCAGGAGCTGCGTAGGCCTGATAAGCGTAGCGCATCAGGCAGTTTGGCGTTTGTCATCAGAGCCAACCACGTCCGCAGACGTGGTTGCTATTCAGATAACGTCGATTTCAGCGACTGACGGGTAAAt                                                                                          >  1:509395/1‑143 (MQ=255)
                                                    gAAAATTCAATATATTGCAGGAGCTGCGTAGGCCTGATAAGCGTAGCGCATCAGGCAGTTTGGCGTTTGTCATCAGAGCCAACCACGTCCGCAGACGTGGTTGCTATTCAGATAACGTCGATTTCAGCGACTGACGGGTAAAt                                                                                          >  1:14196/1‑143 (MQ=255)
                                                    gAAAATTCAATATATTGCAGGAGCTGCGTAGGCCTGATAAGCGTAGCGCATCAGGAAGTTTGGCGTTTGTCATCAGAGCCAACCACGTCCGCAGACGTGGTTGCTCTTCAGATAAGGTCGCTTTCAGCGACTGACGGGTAAAt                                                                                          >  1:29826/1‑143 (MQ=255)
                                                              tatatTGCAGGAGCTGCGTAGGCCTGATAAGCGTAGCGCATCAGGCAGTTTGGCGTTTGTCATCAGAGCCAACCACGTCCGCAGACGTGGTTGCTATTCAGATAACGTCGATTTCAGCGACTGACGGGTAAATCCAGCTgggg                                                                                <  2:673009/143‑1 (MQ=255)
                                                                 atTGCAGGAGCTGCGTAGGCCTGATAAGCGTAGCGCATCAGGCAGTTTGGCGTTTGTCATCAGAGCCAACCACGTCCGCAGACGTGGTTGCTATTCAGATAACGTCGATTTCAGCGACTGACGGGTAAATCCAGCTGGGGCgg                                                                             >  1:401863/1‑143 (MQ=255)
                                                                    gCAGGAGCTGCGTAGGCCTGATAAGCGTAGCGCATCAGGCAGTTTGGCGTTTGTCATCAGAGCCAACCACGTCCGCAGACGTGGTTGCTATTCAGATAACGTCGATTTCAGCGACTGACGGGTAAATCCAGCTGGGGCGGaaa                                                                          <  2:40299/143‑1 (MQ=255)
                                                                    gCAGGAGCTGCGTAGGCCTGATAAGCGTAGCGCATCAGGCAGTTTGGCGTTTGTCATCAGAGCCAACCACGTCCGCAGACGTGGTTGCTATTCAGATAACGTCGATTTCAGCGACTGACGGGTAAATCCAGCTGGGGCGGaaa                                                                          <  2:126237/143‑1 (MQ=255)
                                                                    gCAGGAGCTGCGGAGGCCTGATAAGCGTAGCGCATCAGGCAGTTTGGCGTGTGTCATCAGAGCCAACCACGTCCGCAGACGTGGTTGCTATTCAGATAACGTCGATTTCAGCGACTGACGGGTAAATCCAGCTGGGGCGGaaa                                                                          <  1:397860/143‑1 (MQ=255)
                                                                          gCTGCGTAGGCCTGATATGCGTAGGGCAGCAGGCAGGTTGGCGTTTGTCATCAGGGCCAACCACGTCCGCAGACGTGGTTGCTATTCAGATAACGTCGATTTCAGCGACTGACGGGTAAATTCAGCTGGGGCGGAAAGGCATa                                                                    <  2:689356/143‑1 (MQ=255)
                                                                                  ggCCTGATAAGCGTAGCGCATCAGGCAGTTTGGCGTTTGTCATCAGAGCCAACCACGTCCGCAGACGTGGTTGCTATTCAGATAACGTCGATTTCAGCGACTGACGGGTAAATCCAGCTGGGGCGGAAAGGCATACTGTCGat                                                            <  2:14196/143‑1 (MQ=255)
                                                                                     cTGATAAGCGTAGCGCATCAGGCAGTTTGGCGTTTGTCATCAGCGCCAACCACGTCCGCAGACGTGGTTGCTATTCAGATAACGTCGATTTCAGCGACTGACGGGGAAATCCAGCTGGGGCGGAAAGGCATACCGTCGctatg                                                         >  1:192187/1‑142 (MQ=255)
                                                                                     cTGATAAGCGTAGCGCATCAGGCAGTTTGGCGTTTGTCATCAGAGCCAACCACGTCCGCAGACGTGGTTGCTATTCAGCTAACGTCGATTTCAGCCACTGACGCGTAAATCCAGCTGGGGCGCCAAGGCATCCTCTCgtcagc                                                         >  2:56353/1‑138 (MQ=38)
                                                                                      tGATAAGCGTAGCGCATCAGGCAGTTTGGCGTTTGTCATCAGAGCCAACCACGTCCGCAGACGTGGTTGCTATTCAGATTACGTCGCTTTCAGCGACTGACGGGTACATCCAGGTGGGGCGGAAAAGCGTACTGTCGATTtcg                                                        >  2:244121/1‑143 (MQ=38)
                                                                                      tGATAAGCGTAGCGCATCAGGCAGTTTGGCGTTTGTCATCAGAGCCAACCACGTCCGCAGACGTGGTTGCTATTCAGATAACGTCGATTTCAGCGACTGACGGGTAAAACAAGCGGGGGCGGACAGGCATACTGTCGaactcg                                                        >  2:545169/1‑138 (MQ=39)
                                                                                        aTAAGCGTAGCGCATCAGGCAGTTTGGCGTTTGTCATCAGAGCCAACCACGTCCGCAGACGTGGTTGCTATTCAGATAACATCGATTTCAGCGACTGACGGGTAAATCCAGCTGGGGCGGAAAGGCATCCTGTCGATAtcgtc                                                      >  1:448052/1‑143 (MQ=255)
                                                                                              gTAGCGCATCAGGCAGTTTGGCGTTTGTCATCAGAGCCAACCACGTCCGCAGACGTGGTTGCTATTCAGATAACGTCGATTTCAGCGACTGACGGGTAAATCCAGCTGGGGCGGAAAGGCATACTGTCGATATCGTCGAGcga                                                <  1:679211/143‑1 (MQ=255)
                                                                                                  tgcggcAGGCAGTTTGGCGTTTGGAGTCAGAGCCAACCACGTGCGCAGACGTGGTTGCTATTCAGATAACGTCGATTTCAGCGACTGACGGGTAAATCCAGCTGGGGCGGAAAGGCATACTGTCGATATCGTCGAGCGACGaa                                            <  2:326780/138‑1 (MQ=255)
                                                                                                    taTCTGGCAGTTTGGCGGTTGTCATCAGAGCCAGCCACGTCCGCAGGCGTGGTTGCTATTCAGATAACGTCGATTTCAGCGACTGACGGGTAAATCCAGCTGGGGCGGAAAGGCATACTGTCGATATCGTCGAGCGACGAAac                                          <  2:773766/142‑1 (MQ=255)
                                                                                                      tCAGGCAGTTTCGCGTTTGTCATCAGAGCCAGCCACGTCCGCAGTCGTGGTGCCTATTCCGATAACGTCGATTTCAGCGACTGACGGGTAAATAAAGATGGGGCCGCGGGATAGCGGGTCGATATCGCCGAGCGACGAAacac                                        <  2:401863/143‑1 (MQ=255)
                                                                                                      tCAGGCAGGGTGGCGTTTGTCATCAGAGCCAACCACGTCCGCAGACGTGGTTGCTATTCAGACAACGTCGATTTCAGCGACTGACGGGTAAATCCAGCTGGGGCGGAAAGGCATACTGTCGATATCGTCGAGCGACGAAacac                                        <  1:447924/143‑1 (MQ=255)
                                                                                                      tCAGGCACTGTGGCGGTTGTCATCAGCGCCAACCACTTCCGCAGACGTGGTTGCTATTCAGATAACGTCGATTTCAGCGACTGCCGGGTAAACCCAGCTGGGGCGGAAAGGCATACTGTCGATATCGTCGAGCGACGAAacac                                        <  2:340048/143‑1 (MQ=255)
                                                                                                           cAGTTTGGCGTTTGTCATCAGAGCCAACCACGTCCGCAGCCGTGGTTGCTATTCAGATAACGTCGATTTCAGCGACTGACGGGTAAATCCAGCTGGGGCGGAAAGGCATACTGTCGATATCGTCGAGCGACGAACCCCCAGaa                                   >  2:840130/1‑143 (MQ=255)
                                                                                                              tttGGCGTTTGTCATCAGAGCCAACCACGTCCGCAGACGTGGTTGCTATTCAGATAACGTCGATTTCAGCGACTGACGGGTAAATCCAGCTGGGGCGGAAAGGCCTACTGTCGATTTCGTCGTGCCCCGAAAAACCAGaaacc                                >  1:72890/1‑141 (MQ=255)
                                                                                                              tttGGCGTTTGTCATCAGAGCCAACCACGTCCGCAGACGTGGTTGCTATTCAGATAACGTCGATTTCAGCGACTGACGGGTAAATCCAGCTGGGGCGGAAAGGCATACTGTCGATATCGTCGAGCAACGAAACACCAGAAAGc                                >  1:759963/1‑143 (MQ=255)
                                                                                                                   cGTTTGTCATCAGAGCCAACCACGTCCGCAGACGTGGTTGCTATTCAGATAACGTCGATTTCAGCGACTGACGGGTAAATCCAGCTGGGGCGGAAAGGCATACTGTCGATATCGTCGAGCGACGAAACACCAGAGTGcagcag                           >  1:324835/1‑143 (MQ=255)
                                                                                                                     tttGTCATCAGAGCCAACCACGTCCGCAGACGTGGTTGCTATTCAGATAACGTCGATTTCAGCGACTGACGGGTAAATCCAGCTGGGGCGGAAAGGCATACTGTCGATATCGTCGAGCGACGAAACACCAGAAAGCACCAGaa                         >  2:417634/1‑143 (MQ=255)
                                                                                                                     tttGTCATCAGAGCCAACCACGGCCGCAGACGTGGTTGCTATTCAGATAACGTCGATTTCAGCGACTGACGGGTAAATCCAGCTGGGGCGGAAAGGCATACTGTCGATATCGTCGAGCGACGAAACACCAGAAAGCACCAGaa                         <  1:725970/143‑1 (MQ=255)
                                                                                                                            tcaGAGCCAACCACGTCCGCAGACGTGGTTGCTATTCAGATAACGTCGATTTCAGCGACTGACGGGTAAATCCAGCTGGGGCGGAAAGGCATACTGTCGATATCGTCGCGCGACGAGACACCGGAAAGCACCAGAATCGgctc                  >  1:753764/1‑143 (MQ=255)
                                                                                                                              agagCCAACCACGTCCGCAGACGTGGTTGCTATTCAGATAACGTCGATTTCAGCGACTGACGGGTAAATCCAGCTGGGGCGGAAAGGCATACTGTCGATATCGTCGAGCGACGAAACACCAGAAAGCACCAGACTCGTCTCCa                >  2:895568/1‑143 (MQ=255)
                                                                                                                              agagCCAACCACGTCCGCAGACGTGGTTGCTATTCAGATAACGTCGATTTCAGCGACTGACGGGTAAATCCAGCTGGGGCGGAAAGGCATACTGTCGATATCGTCGAGCGACGAAACACCAGAAAGCACCAGAATCGTCTCCa                >  1:704379/1‑143 (MQ=255)
                                                                                                                                agCCAACCACGTCCGCAGAGGTGGTTGCTATTCAGATAACGTCGATTTCAGCGACTGACGGGTAAATCCAGCTGGGGCGGAAAGGCATACTGTCGATATCGTCGAGCGACGAAACACCAGAAAGCACCAGAATCGTCTCCAGa              <  1:258534/143‑1 (MQ=255)
                                                                                                                                   cAACCACGTCTGCGGACGTGGTTGCTATTCAGATAACGTCGATTTCAGCGACTGACGGGTAAATCCAGCTGGGGCGGAAAGGCATAATGTCGATCTCGTCGAGCGCCGAAACACCAGAAAGCACCAAAAACGACTCCAGaacg           >  2:488490/1‑140 (MQ=255)
                                                                                                                                    aaCCACGTCTGCGGACGTGGTTGCTATTCAGATAACGTCGATTTCAGCGACTGACGGGTAAATCCAGCTGGGGCGGAAAGGCATACTGTCGATATCGTCGAGCGACGAAACACCAGAAAGCACCAGACTCGTCTCCAGAcctg          >  1:359742/1‑143 (MQ=255)
                                                                                                                                     aCCACGTCCGCAGACGTGGTTGCTATTCAGATAACGTCGATTTCAGCGACTGACGGGTAAATCCAGCTGGGGCGGAAAGGCCTACTGTCGATATCGTCGAGCGACGAAACACCAGAAAGCACCAGAATCGTCCCCAGAcctgc         >  2:205951/1‑143 (MQ=255)
                                                                                                                                      ccACGTCCGCAGACGTGGTTGCTATTCAGATAACGTCGATTTCAGCGACTGACGGGTAAATCCAGCTGGGGCGGAAAGGCATACTGTCGATATCGTCGAGCGACGAAACACCAGAAAGCACCAGAATCGTCTCCAGAcctgcc        <  1:191990/143‑1 (MQ=255)
                                                                                                                                         cGTCCGCAGACGTGGTTGCTATTCAGATAACGTCGATTTCAGCGACTGACGGGTAAATCCAGCTGGGGCGGAAAGGCATACTGTCGATATCGTCGAGCGACGAAACACCAGAAAGCACCAGAATCGTCTCCAGACCTGCCTgg     <  1:881442/143‑1 (MQ=255)
                                                                                                                                           tCCGCAGACGTGGTTGCTATTTAGTTAGCGTCGATTTCAGCTACTGACGGGTAAATCCAGCTGGGGGGGGAAGGCATACTGTCGATATCGTCGAGCGACGAAACACCAGAAAGCACCAGAATCGTCTCCAGACCTGCCTGGaa   <  1:111875/143‑1 (MQ=255)
                                                                                                                                            ccGCAGACGTGGTTGCTATTCAGATAACGTCGATTTCAGCGACTGACGGGTAAATCCAGCTGGGGCGGAAAGGCATACTGTCGATATCGTCGAGCGACGAAAGACCAGAAAGCACTAGAATCGACTCCAGAcgtgactctaag  >  1:405054/1‑138 (MQ=255)
                                                                                                                                             |                                                                                                                                             
ATTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACAAAAGCATGAAAATTCAATATATTGCAGGAGCTGCGTAGGCCTGATAAGCGTAGCGCATCAGGCAGTTTGGCGTTTGTCATCAGAGCCAACCACGTCCGCAGACGTGGTTGCTATTCAGATAACGTCGATTTCAGCGACTGACGGGTAAATCCAGCTGGGGCGGAAAGGCATACTGTCGATATCGTCGAGCGACGAAACACCAGAAAGCACCAGAATCGTCTCCAGACCTGCCTGGAAG  >  NC_000913/699415‑699697

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑