Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_000913	800,807	G→T	100%	A17S (GCG→TCG)	ybhH →	putative isomerase YbhH

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	800,807	0	G	T	100.0%	97.3 / NA	35	A17S (GCG→TCG)	ybhH	putative isomerase YbhH
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base T (15/20);  total (15/20)
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

TTATTAATCGTTGAGCTAAAGTCATTAGAGATGCTTTGCCCTTAATGTAACCATATCGCAATAAGTTATGTTTTTAAATTGAGGGCATTATTATGAAAAAAATACCCTGCGTGATGATGCGAGGTGGAACCTCGAGGGGCGCGTTCCTGTTAGCGGAACATTTACCCGAAGATCAAACGCAGCGCGATAAAATATTGATGGCAATTATGGGTTCCGGTAACGATCTGGAAATTGACGGTATTGGCGGCGGTAATCCACTGACCAGTAAAGTCGC  >  NC_000913/800667‑800940                                                                                                                                             |                                                                                                                                      ttattaATCGTTGAGCTAAAGTCATTAGAGATGCTTTGCCCTTAATGTAACCATATCGCAATAAGTTATGTTTTTAAATTGAGGGCATTATTATGAAAAAAATACCCTGCGTGATGATGCGAGGTGGAACCTCGAGGGGctcg                                                                                                                                     >  1:3245/1‑143 (MQ=255)    ttaACCGTTGAGCTAAAGTCATTAGAGATGCTTTGCCCTTAATGTAACCATATCGCAATAAGTTATGTTTTTAAATTGAGGGCATTATTATGAAAAAAATACCCTGCGTGATGATGCGAGGTGGAACCTCGAGGGGCTCGTTc                                                                                                                                  <  1:485680/143‑1 (MQ=255)          gTTGAGCTACAGGCATTAGAAATGCTTTGCCCTTAAAGTAATCATATCGCAATAAGTTATGTTTTTAAATTGAGGGCATTATTAAGAAAAAAATACCCTGCGTGATGATGCGAGGTGGAACCTCGAGGGGCTCGTTCCTGTTa                                                                                                                            <  2:753121/143‑1 (MQ=255)           ttGAGCTAAAGTCATTAGAGATGCTTTGCCCTTAATGTAACAATATCGCAGTAAGTTATGTTTTTAAATTGAGGGCATTATTATGACAAAAATACCCTACCTGCTGATGCGAGGTGGAACCTCGAGGGGCTCGCTCCTGTAAg                                                                                                                           >  2:324584/1‑143 (MQ=255)                      tCATTAGAGATGCTTTGCCCTTAATGTAACCATATCGCAATAAGTTATGTTTTTAAATTGAGGGCATTATTATGAAAAAAATGTGCTACGTGATGATGCGAGGTGGAACCTCGAGGGGATCGTTCCTGTTAGCGGAACATTTa                                                                                                                >  2:535066/1‑143 (MQ=255)                         ttAGAGATGCTTTGCCCTTAATGTAACCATATCGCCATAAGTTATGTTTTTAAATTGAGGGCATTAATATGAAAAACATACCCTGCGAGATGATGCGAGGTGGAACCTCGGGGGGCTCGTTCCTGTAAGCGGAACATTTAccc                                                                                                             >  2:121969/1‑143 (MQ=255)                         ttAGAGATGCTTTGCCCTTAATGTAACCATATCGCAATAAGTTATGTTTTTAAATTGAGGGCATTATTATGAAAAAAATACCCTGCGTGATGATGCGAGGTGGAACCTCGAGGGGCTCGATCCTGTTACCGGACCACATAccc                                                                                                             >  2:610519/1‑143 (MQ=255)                             agaAGCTTTGGACTAAATGTAACCATAGCGCAATAAGATATGTGTTTAAATTGAGGGCATTATAAAGAAGAAAATACCCTGCGTGATGATGCGAGGTGGAACCTCGAGGGGCTCGTTCCTGTTAGCGGAACATTTACCCGAAg                                                                                                         <  2:741448/143‑1 (MQ=255)                               aTGCTTTGCCCTTAATGTAACCATATCGCAATAAGTTATGTTTTTAAATTGAGGGCATTATTATGAAAAAAATACCCTGCGTGATGATGCGAGGTGGAACCTCGAGGGGCTCGTTCCTGTTGGCGGAACATTTCCCCGAAGAt                                                                                                       >  1:242106/1‑143 (MQ=255)                                  cTTTGCCCTTAATGTAACCATATCGCAATAAGTTATGTTTTTAAATTGAGGGCATTATTATGAAAAAAATACTCTGCGTGATGATGCGAGGTGGAACCTCGAGGGGCTCGTTACAGGTAGCGGAAGATATACCCGAAGGTcca                                                                                                    >  2:340359/1‑141 (MQ=255)                                   ttttccattAATGTACTCTTATCGCAATAAGTTATGTTTTTAAATTGAGGGCATTATTATGAAAAAAATACCCTGCGTGATGATGCGAGGTGGAACCTCGAGGGGCTCGTTCCTGTTAGCGGAACATTTACCCGAAGATCaaa                                                                                                   <  2:756650/136‑1 (MQ=255)                                   ttcgCCCTTGACGTAACCATATCGCAATAAGTTATGTTCTTAAATTGAGGGCATTATTATGAAAAAAATACCCTGCGTGATGCTGCGAGGTGGAACCTCGAGGGGCTCGTTCCTGTTAGCGGAACATTTACCCGAAGATCaaa                                                                                                   <  1:119028/140‑1 (MQ=255)                                    ttGCCCTTAATGTAACCATATCGCAATAAGTTATGTTTTTAAATTGAGGGCATTATTATGAAAAAAATACCCTGCGTGATGATGCGAGGTGGAACCTCGAGGGGCTCGTTCCTGTTAGCGGAACATTTACCCGCAGATCAAAc                                                                                                  >  1:41879/1‑143 (MQ=255)                                         cTTAATGTAACCATATCGCAATAAGTTATGTTTTTAAATTGAGGGCATTATTATGAAAAAAATACCCTGCGTGATGATGCGAGGTGGAACCTCGAGGGGCTCGTCCCTGTTAGCGGAACATTTACCCGAAGATCAAACGCAgc                                                                                             <  2:542395/143‑1 (MQ=255)                                          ttAATGTAACCAAACGTGAATCAGTTATGTTTTTAAATTGAGGGGATTATTATGAAAAAAATACCCTGCGTGATGATGCGAGGTGGAACCTCGAGGGGCTCGTTCCTGTTAGCGGAACATTTACCCGAAGATCAAACGCAgcg                                                                                            <  2:529593/143‑1 (MQ=255)                                                         tgCAATTAGTTATGTTTTTAAGTTGAGGGTGTTATTATTAAAAATATACCCTGCGTGGTGATGCGAGGTGGAACGTCGAGGGGCTCGTTCCTGTTAGCGGAACATTTACCCGAAGATCAAACGCAGCGCGATAAAATATTGAt                                                                             <  1:239390/142‑1 (MQ=255)                                                         cGCAATAAGTTATGTTTTTAAATTGAGGGCATTATTATGAAAAAAATACCCTGCGTGATGATGCGAGGTGGAACCTAGGGGGGCTCCTCCCTGTTAGCGGAACATTTACCCGAAGATCAAACGCAGCGCGATAAAATATTGAt                                                                             <  1:847721/143‑1 (MQ=255)                                                             aTAAGTTATGTTTTTAAATTGAGGGCATTATTATGAAAAAAATACCCTGCGTGATGATGCGAGGTGGAACCTCGAGGGGCTCGTTCCTGTTAGCGGAACATTTACCCGAAGATCAAACGCAGCGCGATAAAATATTGATGGCa                                                                         >  1:140504/1‑143 (MQ=255)                                                                    aTGTTTGTGAAGTGAGGGCATTATTATGAAAAAAATACCCTGCGTGATGATGCGAGGTGGAACCTCGGGGGGCTCGTTCCTGTTAGCGGAACATTTACCCGAAGATCAAACGCAGCGCGATAAAATATTGATGGCAATTATgg                                                                  <  1:587636/143‑1 (MQ=255)                                                                          gtAAATTGAGGGCATTATTATGAAAAAAATACCCTGCGTGATGATGCGAGGTGGAACCTCGAGGGGCTCGTTCCTGTTAGCGGAACATTTACCCGAAGATCAAACGCAGCGCGATAAAATATTGATGGCAATTATGGGTTCCg                                                            <  1:738761/142‑1 (MQ=255)                                                                              aTTGAGGGAATTATTATGAAAAAAATACCCTGCGTGATGATGCGAGGTGGAACATCGAGGGGCTCGTTCCTGTTAGCGGAACATTTACCCGAAGATCAAACGCAGCGCGATAAAATATTGATGGCAATTATGGGTTCCGGTaa                                                        <  1:801272/143‑1 (MQ=255)                                                                                 gAGGGCATTATTATGAAAAAAATACCCTGCGTGATGATGCGAGGTGGAACCTCGAGGGGCTCGTTCCTGTTAGCGGAACATTTACCCGAAGATCAAACGCAGCGCGATAAAATATTGATGGCAATTATGGGTTCCGGTAACGa                                                     <  1:818378/143‑1 (MQ=255)                                                                                 gAGGGCATTAGTATGAAAAAAATACCCTGCGTGATGATGCGAGGTGGAACCTCGAGGGGCTCGTTCCTGTTAGCGGAACATTTACCCGAAGATCAAACGCAGCGCGATAAAATATTGATGGCAATTATGGGTTCCGGTAACGa                                                     <  1:818035/143‑1 (MQ=255)                                                                                        ttgttatGAAAAAAATACCCTGCGTGATGAGGCGAGGTGGAACCTCGAGGGGCTCGTTCCTGTTAGCGGAACATTTACCCGAAGATCAAACGCAGCGCGATAAAATATTGATGGCAATTATGGGTTCCGGTAACGATCTGGaa                                              <  1:364826/140‑1 (MQ=255)                                                                                        ttattaTGAAAAAAATACCCTGCGTGATGATGCGAGGTGGAACCTCGAGGGGCTCGTTCCTGTTAGCGGAACATTTACCCGAAGATCAAACGCAGCGCGATAAAATATTGATGGCAATTATGGGTTCCGGTAACGATCTGGaa                                              <  1:64250/143‑1 (MQ=255)                                                                                                          ccTGCGTGATGATGCGAGGTGGAACCTCGAGGGGCTCGTTCCTGTTAGCGGAACATTTACCCGAGGATCAAACGCAGCGCGATAAACTCTTGATGGCACATTTGGGTGAAGGGAAACACCAGGAACTTGACGGTTTTggcggc                            >  2:583364/1‑143 (MQ=255)                                                                                                          ccTGCGTGATGATGCGAGGTGGAACCTCGAGGGGCTCGTTCCTGTTAGCGGAACATTTACCCGAAGATCAAACGCAGCGCGATAAAATATTGATGGCAATTATGGGTTCCGGTAACGATCTGGAAATTGACGCTATTggcggc                            >  2:376930/1‑143 (MQ=255)                                                                                                            tGCGTGATGATGCGAGGTGGAACCTCGAGGGGCTCGTTCCTGTTAGCGGAACATTTACCCGAAGATCAAACGCAGCGCGATAAAATATTGATGGCAATTATGGGTTCCGGTAACGATCTGGAAATTGACGGTATTGGcggcgg                          <  2:610283/143‑1 (MQ=255)                                                                                                            tGCGTGATGATGCGAGGTGGAACCTCGAGGGGCTCGTTCCTGTTAGCGGAACATTTACCCGAAGATCAAACGCAGCGCGATAAAATATTGATGGCAATTATGGGTTCCGGTAACGATCTGGAAATTGACGGTATTGGcggcgg                          <  2:401062/143‑1 (MQ=255)                                                                                                             gCGTGATGATGCGAGGTGGAACCTCGAGGGGCTCGTTCCTGTTAGCGGAACATTTACCAGAAGATCAAACGCAGCGCGATAAAATATTGATGGCAATTATGAGTTCAGGTAACGATCTGGAAATTGACGGTATTGGCGGCGGt                         >  2:175193/1‑143 (MQ=255)                                                                                                                tgatgaTGCGAGGTGGAACCTCGAGGGGCTCGTTCCTGTTAGCGGAACATTTACCCGAAGATCAAACGCAGCGCGATCTGGTGCTGTTGCCAACTATGGGTTCCGGTCACGATCTGGAAATTGCCGGTATTGGCGGCGGTAAt                      >  1:203913/1‑143 (MQ=255)                                                                                                                 gatgatGCGAGGTGGAACCTCGAGGGGCTCGTTCCTGTTAGCGGAACATTTACCCGAAGATCAAACGCAGCGCGATAAAATATTGATGGCAATTATGGGTTCCGGTAACGATCTGGAAATTGACGGTATTGGCGGCGGTAATc                     <  1:878507/143‑1 (MQ=255)                                                                                                                     atgCGAGGTGGAACCTCGGGGGGCTCGTTCCTGTTCGCGGAACAGTTACCCGAAGATCAAACGCAGCGCGATAAAATATTGATGGCAATTATGGGTTCCGGTAACGATCTGGAAATTGACGGTATTGGCGGCGGTAATCCACt                 <  1:98257/143‑1 (MQ=255)                                                                                                                            gTGGAACCTCGAGGGGCTCGTTCCTGTTAGCGGAACATTTACCCGAAGATCAAACGCAGCGCGATAAAATATTGATGGCAATTATGGGTTCCGGTAACGATCTGGAAATTGACGGTATTGGCGGCGGTAATCCACTGACCAGt          >  2:369422/1‑143 (MQ=255)                                                                                                                                  ccTCGAGGGGCTCGTTCCTGTTAGCGGAACATATACACGAAGAGCAAACGCAGCGCGATAAAATATTGATGGCAATTATGGGTTCCGGTAACGATCTGGAAATTGACGGTATTGGCGGCGGTAATCCACTGACCAGTAGAGTc    >  2:191859/1‑143 (MQ=255)                                                                                                                                    tCGAGGGGCTCGTTCCTGTTAGCGGAACATTTACCCGAAGATCAAACGCAGCGCGATAAAATATTGATGGCAATTATGGGTTCCGGTAACGCTCTGGCAATTGACGGTATTGGCGGCGGTAATCCACTGACCAGTAAAGTCGc  >  1:439364/1‑143 (MQ=255)                                                                                                                                             |                                                                                                                                      TTATTAATCGTTGAGCTAAAGTCATTAGAGATGCTTTGCCCTTAATGTAACCATATCGCAATAAGTTATGTTTTTAAATTGAGGGCATTATTATGAAAAAAATACCCTGCGTGATGATGCGAGGTGGAACCTCGAGGGGCGCGTTCCTGTTAGCGGAACATTTACCCGAAGATCAAACGCAGCGCGATAAAATATTGATGGCAATTATGGGTTCCGGTAACGATCTGGAAATTGACGGTATTGGCGGCGGTAATCCACTGACCAGTAAAGTCGC  >  NC_000913/800667‑800940

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑