| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_000913 | 53,379 | T→C | 13.0% | E13G (GAG→GGG) | pdxA ← | 4‑hydroxythreonine‑4‑phosphate dehydrogenase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 53,379 | 0 | T | C | 13.0% | 89.8 / 6.1 | 46 | E13G (GAG→GGG) | pdxA | 4‑hydroxythreonine‑4‑phosphate dehydrogenase |
| Reads supporting (aligned to +/- strand): ref base T (20/19); new base C (5/2); total (25/21) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.28e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.42e-01 | |||||||||||
AAGGGCGGAGGGTGAGCGGCAAACCGAGCATCGCTGCCCGGTTGGTAAGGAGAGTGGCATCGGCACAAACAACCAGTTCGACCGGCCACTCACGCTGTGCAAGCTGGACAACTAAGTCCGGGCCAATCCCGGCGGGCTCGCCGGGAGTGATCACAACACGTTGGGTTTTAACCATTAGTTGCTCAGGATTTTAACGTAGGCGCTGGCACGTTGTTCCTGCATCCAGCTTGCTGCTTCTTCCGAGAACTTACGGTTCATCAGCATGCGGTATG > NC_000913/53242‑53513 | gaGGGCGGGGGGTGAGCGGCAAACCGAGCAGCGCTGCCCGGTTGGTAAGGAGAGTGGCGTCGGCGCAAACAGCCAGTTCGACCGGCCACTCACGCTGTGCAAGCTGGACAACTAAGTCCGGGCCAATCCCGGCGGGCTCGCCg < 2:219925/142‑1 (MQ=255) ggCGGAGGGTGAGCGGCAAACCGAGCATCGCTGCCCGGTTGGTAAGGGGAGTGGCATCGGCACAAACAACCAGTTCGACCGGCCACTCACGCTGTGCAAGCTGGACAACTAAGTCCGGGCCAATCCCGGCGAGCCCGCCGGGa < 1:455807/143‑1 (MQ=255) ggAGGGTGAGCGGCAAACCGAGCATCGCTGCCCGGTTGGTAAGGAGAGTGGCATCGGCACAAACAACCAGTGCGACCGGCCACTCACGCTGTGCAAGCTGGACAACTAAGTCCGGGCCAATCCCGGCGAGCCCGCCGGGATTg < 1:409648/143‑1 (MQ=255) aGGGTGAGCGGCAAACCGAGCATCGCTGCCCGGTTGGTAAGGAGAGTGGCATCGGCACAAACAACCAGTTCGACCGGCCACTCACGCTGTGCAAGCTGGACAACTAAGTCCGGGCCAGTCCCGGCGGGCTCGCCGGGAGTGAt > 1:627763/1‑143 (MQ=255) gTGAGCGCCAAACCGAGCACCGCTGCCCGGTTGGTAAGGAGAGTGGCATCGGCACAAACAACCAGTTCGACCGGCCACTCACGCTGTGCACGCTGGAAAACTAAGTCCGGTCCAATCCCGGCGGGCTCGCCGGGAGTGATcac < 1:169869/143‑1 (MQ=255) aGCGGCAAACCGAGCATCGCTGCCCGGTTGGTAAGGAGAGTGGCATCGGCACAAACAACCAGTTCGACCGGCCACTCACGCTGTGCAAGCTGGACAACTAAGTCCGGGCCAATCCCCGCGGCCCCGCCGAGGGTGagcgggac > 2:772478/1‑136 (MQ=255) ggCAAACCGAGCATCGCTGCCCGGTTGGTAAGGAGAGTGGCATCGGCACAAACAACCAGTGCGACCGGCCACTCACGCTGTGCAAGCTGGACAACTAAGTAAGAGCCAATCCCGGCGGGCTCGCGGGAGGGGGACACAACACg > 2:690473/1‑143 (MQ=255) gCAAACCGAGCATCGCTGCCCGGTTGGTAAGGAGAGTGGCATCGGCACAAACAACCAGTTCGACCGGCCACTCACGCTGTGCAAGCTGGACAACTAAGTCCGGGCCAATCCCGGCGGGCTCGCCGGGAGGGATGACAACACGt > 1:195981/1‑143 (MQ=255) aCCGAGCATCGCTGCCCGGTTCGTAAACCGAATAAGGTTTTGACGAACAACCAGTTCGACCGGCCACTCACGCTGTGCAAGCTGGACAACTAAGTCCGGGCCAATCCCGGCGGGCTCGCCGGGAGTGATCACAACACGTTggg < 1:842939/143‑1 (MQ=255) ccGAGCATCGCTGCCCGGTTGGTAAGGAGAGTGGCATCGGCACAAACAACCAGTTCGACCGGCCACTCACGCTGTGCAAGCTGGACAACTAAGTCCGGGCCAATCCCGGCGGGCTCGCCGGGAGTGATCACAACACGTTGGGt < 1:516943/143‑1 (MQ=255) cgcgCAGCGCGGCCCGGTTGGTAAGGAGAGTGGCATCGGCACAAACAACCAGTTCGACCGGCCCCTCACGCTGTGCAGGCTGGACAACTAAGTCCGGGCCAATCCCGGCGGGCTCGCCGGGAGTGATCACAACACGTTGGGtt < 1:399488/140‑1 (MQ=255) cATCGCTGCCCGGTTGGTAAGGAGAGTGGCATCGGCACAAACAACCAGTTCGACCGGCCACTCACGCTGTGCAAGCTGGACAACTAAGTCCGGGCCAATCCCGGCGGGCTCGCCGGGAGTGATCACAACACGTTGGGTTTTaa < 1:353342/143‑1 (MQ=255) aTCGCTGCCCGGTTGGTAAGGAGAGTGGCATCGGCACAAACAACCAGTTCGACCGGCCACTCACGCTGTGCAAGCTGGACAACTAAGTCCGGGCCAATCCCGGCGGGCTCGCCGGGAGCGATCCCAACCCGTTGGGTCTTCAc > 2:11086/1‑143 (MQ=255) tCGCTGCCCGGGTAGTAAGGAGAGTGGCATCGGCACAAACAACCAGTTCGACCGGCCACTCACGCTGTGCAAGCTGGACAACTAAGTCCGGGCCAATCCCGGCGGGCTCGCCGGGAGTGATCACAACACGTTGGGTTTTAAcc < 1:316690/143‑1 (MQ=255) cccGGTTGGTAAGGAGAGTGGCATCGGCACAAACAACCAGTTCGACCGGCCACTCACGCTGTGCAAGCTGGACAACTAAGTCCGGGCCGATCCCGGCGGGCTCGCCGGGAGTGATCACGACACGTTGGGTTTTAACCATTAGt > 1:32559/1‑143 (MQ=255) cccGGTTGGTAAGGAGAGTGGCATCGGCACAAACAACCAGTTCGACCGATCTCTCACGCTGTGCAAGCTGGACAACTAAGTCCGGGCCAATCCCGGCGGGCTCGCCGGGAGTGATCACAACACGTTGGGTTTTAACCACTAGt > 2:636476/1‑143 (MQ=255) ccGGTTGGTAAGGAGAGTGGCATCGGCACAAACAACCAGTTCGACCGGCCACTCACGATGTGCAAGCTGGACAACTAAGTCCGGGCCAATCCCGGCGGGCTCGGCGGGAGTGATCACAACACGTTGGGTTTTAGCCATTAGtt > 2:82166/1‑143 (MQ=255) ttcgTAAGGAGAGTGGCATCGCCACAAACACCCAGTTCGACCGGCCACTCACGCTGTGCAAGCTGGACAACTAAGTCCGGGCCAATCCCGGCGGGCTCGCCGGGAGTGATCACAACACGTTGGGTTTTAACCATTAGTTGCTc < 1:269705/140‑1 (MQ=255) cggcaaGGAGAGTGGCATCGGCACAAACAACCAGTTCGACCGGCCACTCACGCTGTGCAAGCTGGACAACTAAGTCCGGGCCAATCCCGGCGGGCTCGCCGGGAGTGATCACAACACGTTGGGTTTTAACCATTAGTTGCTCa < 1:248928/139‑1 (MQ=255) tAAGTAGAGTGGCATCGGCACAAACAACCAGTTCGACCGGCCACTCACGCTGTGCAAGCTGGACAACTAAGTCCGGGCCAATCCCGGCGGGCTCGCCGGGAGTGATCACAACACGTTGGGTTTTAACCATTAGTTGCTCAGGa < 1:373434/143‑1 (MQ=255) ggAGAGTGGCATCGGCACAAACAACCAGTTCGACCGGCCACTCACGCTGTGCAAGCTGGACAACTAAGTCCGGGCCAATCCCGGCGGGCTCGCCGGGCGTGATCACAACACGTTGGGTTTTAACCATTAGTTGCTCCGGCttt > 1:340252/1‑143 (MQ=255) gagaGTGGCATCGGCACAAACAACCAGTTCGACCGGCCACTCACGCTGTGCAAGCTGGGCAACTAAGTCCGGGCCAATCCCGGCGGGCCCGCCGGGAGGGAACACAACACGTCGGGCCTTAACCGCGAGTTGCTCAggcatct > 2:3305/1‑138 (MQ=255) acaacCAGTTCGACCGGCCACTCACGCTGTGCAAGCTGGACAACTAAGTCCGGGCCAATCCCGGCGGGCTCGCCGGGAGTGATCACAACACGTTGGGTTTTAACCATTAGTTGCTCAGGATTTTAACGTGGGCGCTGGCACgt > 1:399192/1‑143 (MQ=255) taGTTCGACCGGCCACTCACGCTGTGCAAGCTGGACAACTAAGTCCGGGCCAATCCCGGCGGGCTCGCCGGGAGTGATCACAACACGTTGGGTTTTAACCATTAGTTGCTCAGGATTTTAACGTAGGCGCTGGCACGTTGTTc < 1:855864/142‑1 (MQ=255) gTTCGACCGGCCACTCAGGCGGTGCAAGCTGGACAACTAAGTCCGGGCCAATCCCGGCGGGCTCGCCGGGAGTGATCACAACACGTTGGGTTTTAACCATTAGTTGCTCAGGATTTTAACGTAGGCGCTGGCACGTTGTTCCt < 2:859231/143‑1 (MQ=255) ccGGCCACTCACGCTGTGCAAGCTGGGCAACTAAGTCCGGGCCAGTCCCGGCGGGCTCGCCGGGAGTGATCACAACACGTTGGGTTTTAACCATTAGTTGCTCAGGATTTTAACGTAGGCGCTGGCACGTTGTTCAAGCATcc < 2:834687/143‑1 (MQ=255) ccGGCCACTCACGCTGTGCAAGCTGGACAACTAAGTCCGGGCCAATCCCGGCGGGCTCGCCGGGAGTGATCCCAACACGTTGGGTGTGAACCATGAGTTGCTCACGATATTAACGGAGGCGCGGCCACGTTGTTccgcgctca > 2:55740/1‑136 (MQ=255) ggCCACTCACGCTGTGCAAGCTGGACAACTAAGTCCGGGCCAATCCCGGCGGGCTCGCCGGGAGTGATCACAACACGTTGGGTTCTAACCATTAGTTGGCCAGGAGTTTGACGTACGCGCCGGCACGTTGTTCGTGCATcccg > 2:99483/1‑141 (MQ=255) gCCACTCACGCTGTGCAAGCTGGACAACTAAGTCCGGGCCAATCCCGGCGGGCTCGCCGGGAGTGATCACAACACGTTGGGTTTTAACCCTTCGTTGCGTAGGATTTTAACGTAGGCGCCAGCACGTTGTTCCTGCATCCAGc > 2:307736/1‑143 (MQ=255) aCTCACGCTGTGCAAGCTGGACAACTAAGTCCGGGCCAATCCCGGCGGGCTCGCCGGGAGTGATCACAACACGTTGGGTTTTAACCATTAGTTGCTCAGGATTTTAACGTAGGCGCTGGCACGTTGTTGCTGCCTGCAGCTtg > 1:116504/1‑143 (MQ=255) tCACGCTGTGCAAGCTGGACAACTAAGTCCGTGCCAATCCCGGCGGGCTCGCCGGGAGTGATCACAACACGTTGGGTTTTAACCATTAGTTGCTCAGGATTTTAACGTAGGCGCTGGCACGTTGTTCCTGCATCCAGCTtgct < 1:504253/143‑1 (MQ=255) gCTGTGCAAGCTGGACAACTAAGTCCGGGCCAATCCCGGCGGGCTCGCCGGGAGTGATCACAACACGTTGGGTTTTAACCATTAGTTGCTCAGGATTTTAACGTAGGCGCTGGCACGTTGTTCCTGCATCCAGCTTGCTGctt > 1:803634/1‑143 (MQ=255) aaGCTGGACAACTAAGTCCGGGCCAATCCCGGCGGGCTCGCCGGGAGTGATCACAACACGTTGGGTTTTAACCATTAGTTGCTCAGGATTTTAACGTAGGCGCTGGCACGTTGTTCCTGCATCCAGCTTGCTGCTTCTTCCga < 1:101636/143‑1 (MQ=255) aaGATGGACAACTAAGTCCGGGCCAATCACGGCGCGCTCGCCGGGAGTGATCACAACACGTTGGGTCTTAACCATTAGTTGACCAGGATTTTAACGTCGGCGCTGGCACGCTGTTCCTCCATGCAGCTTACCGCTACTTACgc > 2:594948/1‑142 (MQ=255) ggACAACTAAGTCCGGGCCAATCCCGGCGGGCTCGCCGGGAGTGATCACAACACGCTGGGTTTTAACCATTGGTTGATCAGGCTTTTAACGTAGGCGCTGGCACGTTGTTCCTGCATCCAGCTTGCTGCTTCGTCCGAGAACt > 2:239309/1‑143 (MQ=255) gcaggtAAGTCCGGGCCAACCCCGGCGGGCTCGCCGGGAGTGCTCACAACACGTTGGGTTTTAACCATTAGTTGCTCAGGATTTTAACGTAGGCGCTGGCACGTTGTTCCTGCATCCAGCTTGCTGCTTCTTCCGAGAACTTa < 1:502168/138‑1 (MQ=255) cTAAGTCCGGGCCAATCCCGGCGGGCTCGCCGGGAGTGATCCCCACACGTTGAGTTTTAACCAGTAAGCGCCCAGGATCGTAACGAAGGCGCTGGCACGTTGGTCCTGCCTCCAGCTTGCTGCTTCTTACGCGAACTATCGGt > 2:159175/1‑143 (MQ=255) cTAAGTCCGGGCCAATCCCGGCGGGCTCGCCGGGAGTGATCACAACACGTTGGGTTTTAACCATTAGTTGCTCAGGATTTTAACGTAGGCGCTGGCACGTTGTTCCTGCATCCAGCTTGCTGCTTCTTCCGAGAACTTACGGt > 2:644196/1‑143 (MQ=255) ccGGGCCAATCCCGGCGGGCTCGCCGGGAGTGATCACAACACGTTGGGTTTTAACCATTAGTTGCTCAGGATTTTAACGTAGGCGCTGGCACGTTGTTCCTGCATCCAGCTTGCTGCTTCTTCCGAGACCTTACGGTtcatca < 1:750924/143‑1 (MQ=255) gagCCAATCCCGGCGGGCTCGCCGGGAGTGATCACAACACGTTGGGTTTTAACCATTAGTTGCTCAGGATTTTAACGTAGGCGCTGGCACGTTGTTCCTGCATCCAGCTTGCTGCTTCTTCCGAGAACTTACGGTTCATCAGc < 1:150252/141‑1 (MQ=255) actCCCGGCGAGCCCGCCGGGAGTGATCACAACACGTTGGGTTTTAACCATTAGTTGCTCAGGATTTTAACGTAGGCGCTGGCACGTTGTTCCTGCATCCAGCTTGCTGCTTCTTCCGAGAACTTACGGTTCATCAGCATGCg > 2:569601/3‑143 (MQ=255) actCCCGGCGAGCCCGCCGGGAGTGATCACAACACGTTGGGTTTTAACCATTAGTTGCTCAGGATTTTAACGTAGGCGCTGGCACGTTGTTCCTGCATCCACCTTGCTGCTTCTTCCGAGAACTTGCGGTTCATCCGCAGGCg > 1:807448/3‑143 (MQ=255) aTCCCGGCGGGCTCGCCGGGAGTGATCACAACACGTTGGGTTTTAACCATTAGTTGCTCAGGATTTTAACGTAGGCGCTGGCACGTTGTTCCTGCATCCAGCTTGCTGCTTCTTCCGAGAACTTACGGTTCATCAGCCTGcag < 2:364431/143‑3 (MQ=255) tCCCGGCGAGCCCGCCGGGAGTGATCACAACACGTTGGGTTTTAACCATTAGTTGCTCAGGATTTTAACGTAGGCGCTGGCACGTTGTTCCTGCATCCAGCTTGCTGCTTCTTCCGAGAACGTACGGTTCATCAGCATGCGGt > 2:390182/1‑143 (MQ=255) ccGGCGGGCTCGCCGGGAGTGATCACAACACGTTGGGTTTTAACCACTAGTTGCTCAGGATTTTAACGCAGGCGCTGGCACGTTGTTCCTCCATCCAGCTTGCTGCTTCTTCCGAGCACGTACGGTTCATCAGCGTGCgggaa > 2:674079/1‑140 (MQ=255) cggcggGCTCGCCGGGAGTGATCACAACACGTTGGGTTTTAACCATTAGTTGCTCAGGATTTTAACGTAGGCGCTGGCACGTTGTTCCTGCATCCAGCTTGCTGCTTCTTCCGAGAACTTACGGTTCGTCAGCATGCGGtgtg > 1:539245/1‑143 (MQ=255) | AAGGGCGGAGGGTGAGCGGCAAACCGAGCATCGCTGCCCGGTTGGTAAGGAGAGTGGCATCGGCACAAACAACCAGTTCGACCGGCCACTCACGCTGTGCAAGCTGGACAACTAAGTCCGGGCCAATCCCGGCGGGCTCGCCGGGAGTGATCACAACACGTTGGGTTTTAACCATTAGTTGCTCAGGATTTTAACGTAGGCGCTGGCACGTTGTTCCTGCATCCAGCTTGCTGCTTCTTCCGAGAACTTACGGTTCATCAGCATGCGGTATG > NC_000913/53242‑53513 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |