| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_000913 | 962,670 | A→G | 22.7% | K226E (AAA→GAA) | rpsA → | 30S ribosomal subunit protein S1 |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 962,670 | 0 | A | G | 22.7% | 87.1 / 23.4 | 57 | K226E (AAA→GAA) | rpsA | 30S ribosomal subunit protein S1 |
| Reads supporting (aligned to +/- strand): ref base A (21/23); new base G (7/6); total (28/29) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 7.60e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.42e-01 | |||||||||||
AGAGCGCGATCAGCTGCTGGAAAACCTGCAGGAAGGCATGGAAGTTAAAGGTATCGTTAAGAACCTCACTGACTACGGTGCATTCGTTGATCTGGGCGGCGTTGACGGCCTGCTGCACATCACTGACATGGCCTGGAAACGCGTTAAGCATCCGAGCGAAATCGTCAACGTGGGCGACGAAATCACTGTTAAAGTGCTGAAGTTCGACCGCGAACGTACCCGTGTATCCCTGGGCCTGAAACAGCTGGGCGAAGATCCGTGGGTAGCTATCGCTAA > NC_000913/962534‑962809 | agagCGCGATCAGCTGCTGGAAAACCTGCAGGAAGGCGTGGAAGTTAAAGGTATCGTTAAGAACCTCACTGACTACGGTGCATTCGTTGATCTGGGCGGCGTTGACGGCCTGCTGCACATCACTGACATGGCCTGGAAAcgcg < 2:537724/143‑1 (MQ=255) gggcgcGATCAGCTGGTCGAAAACCTGCAGGAAGGCATGGAAGTTAAAGGTATCGTTAAGAACCTCACTGACTACGGTGCATTCGTTGATCTGGGCGGCGTTGACGGCCTGCTGCACATCACTGACATGGCCTGGAAACGCGt < 2:829524/141‑1 (MQ=255) gagCGCGGTCACTTGCTGGAATACCTGCAGGAAGGCATGGAAGTTAAAGGTATCGTTAAGAACCTCACTGCCTACGGTGCATTCGTTGATCTGGGCGGCGTTGACGGCCTGCTGCACATCACTGACATGGCCTGGAAACGCGt < 2:32163/143‑1 (MQ=255) agCGCGATCAGCTGCTGGAAAACCTGCAGGAAGGCATGGAAGTTAAAGGTATCGTTAAGAACCTCACTGACTACGGTGCATTCGTTGATCTGGGCGGCGTTGACGGCCTGCTGCACATCACTGACATGGCCTGGAAACGCGtt > 1:70542/1‑143 (MQ=255) aTCAGCTGCTGGAAAACCTGCAGGAAGGCATGGAAGTTAAAGGTATCGGTAAGAACCTCACTGACTACGGTGCATTCGTTGATCTGGGCGGCGTTGACGGCCTGCTGCACATCACTGACATGGCCTGGGAACGCGTTAAGCAt > 1:460844/1‑143 (MQ=255) aGCTGCTGGAAAACCTGCAGGAAGGCATGGAAGTTAAAGGTATCGTTAAGAACCTCACTGACTACGGTGCATTGGGTGATCTGGGCGGCGTTGACGGCCTGCTGCACATCACGGACATGGCATGGACACGCGTTACGCATCCg > 1:394842/1‑143 (MQ=255) aGCTGCTGGAAAACCTGCAGGAAGGCATGGAAGTTAAAGGTATCGTTAAGAACCTCACTGACTACGGTGCATTCGTTGATCTGGGCGGCGTTGACGGCCTGCTGCACATCACTGACATGGCCTGGAAACGCGTTAAGCATCCg > 2:517293/1‑143 (MQ=255) tgctgGAAAACCTGCAGGAAGGCATGGAAGTTAAAGGTATCGTTAAGAACCTCACTGACTACGGTGCATTCGTTGATCTGGGCGGCGTTGACGGCCTGCTGCACATCACTGACATGGCCTGGGAACGCGTTCAGCATCGGAGc > 2:342090/1‑143 (MQ=255) aaaaCCTGCAGGAAGGCATGGAAGTTAACGGTATCGTTAAGAACCTCACTGACTACGGTGCATTCGTTGATCTGAGCGGCGTTGACGGCCTGCTGCACATCACTGACATGGCCTGGAAACGCGCTAAGCATCCGAGCGAAATc > 2:99159/1‑143 (MQ=255) aaaCCTGCAGGAAGGCATGGAAGTTAAAGGTATCGTTAAGAACCTCACTGACTACGGTGCATTCGTTGATCTGGGCGGCGTTGACGGCCTGCTGCACATCACTGACATGGCCTGGAAACGCGTTAAGCATCCGAGCGAAATCg > 2:645624/1‑143 (MQ=255) aaCCTGCAGGAAGGCATGGAAGTTAAAGGTATCGTTAAGAGCCTCACTGACTACGGTGCATTCGTTGATCTGGGCGGCGTTGACGGCCTGCTGCACATCACTGACATGGCCTGGAAACGCGTTAAGCATCCGAGCGAAATCGt < 2:408284/143‑1 (MQ=255) ggAAGTCAGGGAAGTTAAACGTATCGTTAAGAACCTCACTGACTACGGTGCATTCGTTGATCTGGGCGGCGTTGACGGCCTGCTGCACATCACTGACATGGCCTGGAAACGCGTTAAGCATCCGAGCGAAATCGTCAACGTgg < 2:738472/143‑1 (MQ=255) ggAAGGCATGGAAGTTAAAGGTATCGTTAAGAACCTCACTGACTACGGTGCATTCGTTGATCTGGGCGGCGTTGACGGCCTGCTGCACATCACTGACATGGCCTGGGAACGCGTTAAGCATCCGAGCGAAATCGTCAACGTgg < 1:765622/143‑1 (MQ=255) gggaTGCAAGTTAAAGGTATCGGTAAGAACCGCACTGACTACGGTTCATTCGTTGATCTGGGCGGCGTTGACGGCCTGCTGCACATCACTGACATGGCCTGGAAACGCGTTAAGCATCCGAGCGAAATCGTCAACGTGGGcga < 2:359151/140‑1 (MQ=255) gCATGGAAGTTAAAGGTATCGTTAAGAACCTCACTGACTACGGTGCATTCGTTGATCTGGGCGGCGTTGACGGCCTGCTGCACATCACTGACATGGCCTGGAAACGCGTTAAGCATCCGAGCGAAATCGTCAACGTGGGcgac > 1:460053/1‑143 (MQ=255) aTGGAAGTTAAAGGTATCGTTAAGAACCTCACTGACTACGGTGCATTCGTTGATCTGGGCGGCGTTGACGGCCTGCTGCACATCACTGACATGGCCTGGAAACGCGTTAAGCATCCGAGCGAAATCGTCAACGTGGGcgacga > 1:657825/1‑143 (MQ=255) aTGGAAGTTAAAGGTATCGTTAAGAACCTCACTGACTACGGTGAATTCGTTGATCTGGGCGGCGTTGACGGCCTGCTGCACATCACTGACATGGCCTGGAAACGCGTTAAGCATCCGAGTGAACTGGTCAAGTTGGGcgtcgg > 1:469536/1‑142 (MQ=255) aGTTAAAGGTATCGTTAAGAACCTCACTGGCTACGGTGCATTCGTTGAGCTGGGCGGCGTTGACGGCCTGCGGCACATCACTGACATGGCCTGGAAACGCGTTAAGCATCCGAGCGAAATCGTCAACGTGGGCGACGAAATCa < 2:567026/143‑1 (MQ=255) gtAAAGGTATCGGTAAGAACCTCACTGACGACGGGGCATTCGTCGATCTGGGCGGCGTTGACGGCCTGCTGCACATCACTGACATGGCCTGGAACCGCGTTAAGCATCCGAGCGAAATCGTCAACGTGGGCGACGAAATCACt < 1:225039/142‑1 (MQ=255) cGTTAAGAACCTCACTGACTACCGTGCATTCGTTGATCTGGGCGGCGTTGACGGCCTGCTGCACATCACTGCCATCGCCTGGGAACGCGTTAAGCATCCGAGCGAAATCGTCAACGTGGGCGACGAAATCACTGTTAAAGTGc < 1:15814/143‑1 (MQ=255) aaGAACCTCACTGACTACGGTGCATTCGTTGATCTGGGCGGCGTTGACGGCCTGCTGCACATCACTGACATGGCCTGGAAACGCGTTAAGCATCCGAGCGAAATCGTCAACGTGGGCGACGAAATCACTGTTAAAGTGCTGaa > 1:482920/1‑143 (MQ=255) ccTCACTGACTACGGTGCATTCGTTGATCTGGGCGGCGTTGACGGCCTGCTGCACATCACTGACATGGCCTGGAAACGCGTTAAGCATCCGAGCGAAATCGTCAACGTGGGCGACGAAATCACTGTTAAAGTGCTGAAGTTCg > 1:762659/1‑143 (MQ=255) ccTCACTGACTACGGTGCATTCGTTGATCTGGGCGGCGTTGACGGCCTGCTGCACATCACTGACATGGCCTGGAAACGCGTTAAGCATCCGAGCGAAATCGTCAACGTGGGCGACGAAATCACTGTTAAAGTGCTGAAGTTCg < 1:439154/143‑1 (MQ=255) ctctctGACTCCGGTGCATTCGTTGATCTGGGCGGCGGGGACGGGCTGCTGCACATCACTGACATGGCCTGGAAACGCGTTAAGCATCCGAGCGAAATCGTCAACGTGGGCGACGAAATCACTGTTAAAGTGCTGAAGTTCGa < 2:553495/143‑1 (MQ=255) ctcaaggACTACGGTGCATTCGTTGGTCGGGGCGGCGTTGACGGACTGCTGCACATCACTGACATGACCTGGGGACGCGTTAAGCACCCGGGCGAAATCGTCAACGTGGGCTGCTAACTCACTGTTAAAGTGCTGACGTGCGa > 2:879613/7‑143 (MQ=255) cTCACTGACTACGGTGCATTCGTTGATCTGGGCGGCTTTTACGGCCTGCTGCACATCTCTGATATTGCCTGGAAACGCGTTAAGCATCCGAGCGAAATCGTCAACGTGGGCGACGAAATCACTGTTAAAGTGCTGAAGTTCGa < 1:155961/143‑1 (MQ=255) tACGGTGCATTCGTTGATCTGGGCGGCGTTGACGGCCTGCTGCACATCACTGACATGGCCTGGGAACGCGGTAAGCATCCGAGCGAAATCGTCAACGTGGGCGACGAAATCACTGTCAAAGTGCGGAAGTTCGACCGCGAACg > 2:727561/1‑143 (MQ=255) ggTGCAGTCGTTGATCTGGGCGGCGTTGACGGCCTGCTGCACATCACTGACATGGCCTGGGAACGCGTTAAGCATCCGAGCGAAATCGTCAACGTGGGCGACGAAATCACTGTTAAAGTGCTGAAGTTCGACCGCGAACGTAc < 1:831993/143‑1 (MQ=255) tGCATTCGTTGGTCTGGCCGGCGTCGACGGCCTTCTGCACATCACTGACATGGCCTGGGAACGCGTTAAGCATCCGAGCGAAATCGTCAACGTGGGCGCCGAAATCACTGTTAAAGTGCTGAAGTTCGACCGCGAACGTAccc < 1:702543/143‑1 (MQ=255) aactCGTTGATCTGGGCGGCGTGGGCGGCCTGCTGCACATCACTGACTTGGCCTGGAAACGCGTTAAGTATCCGAGCGAAATCGTCAACGTGGGCGACGAAATCACTGTTAAAGTGCTGAAGTTCGACCGCGAACGTACCCgt < 1:119164/140‑1 (MQ=255) aTTCGTTGATCTGGGCGGCGTTGACGGCCTGCTGCACATCACTGACATGGCCTGGAAACGCGTTAAGCATCCGAGCGAAATCGTCAACGTGGGCGACGAAATCACTGTTAAAGTGCTGAAGTTCGACCGCGAACGTAGCCgtg > 1:281585/1‑143 (MQ=255) cGTTGATCTGGGCGGCGTTGACGGCCTGCTGCACATCACTGACATGGCCTGGAAACGCGTTAAGCATCCGAGCGAAATCGTCAACGTGGGCGACGAAATCACTGTTAAAGTGCTGAAGTTCGACCGCGAACGTACCCGTGTAt < 1:21876/143‑1 (MQ=255) gTTGATCTGGGCGGCGTTGACGGCCTGCTGCACATCACAGACATGGCCTGGAAACGCGTTAAGCATACGAGCGAAATCGACAACGTGGGCGACGAAATCACTGTTAAAGTGCTGAAGTTCGACCGCGAACGTACGCGTGtctc > 2:334582/1‑143 (MQ=255) gATCTGGGCGGCGTTGACGGCCTGCTGCACATCACTGACATGGCCTGGAAACGCGTTAAGCATCCGAGCGAAATCGTCAACGTGGGCGACGAAATCACTGTTAAAGTGCTGAAGTTCGACCGCGAACGTACCCGTGTATCCCt < 1:517293/143‑1 (MQ=255) aTCTGGGCGGCGTTGACGGCCTGCTGCACATCACTGACATGGCCTGGAAACGCGTTAAGCATCCGAGCGAAATCGTCAACGTGGGCGACGAAATCACTGTTAAAGTGCTGAAGTTCGACCGCGAACGTACCCGCGTATCCCTg > 2:594324/1‑143 (MQ=255) aTCTGGGCGGCGTTGACGGCCTGCTGCACATCACTAACATGGCCTGGAAACGCGTTAAGCATCCGATCGACATCATCAACGTGGGCGGCGAAATCACTGTTAAAGTGCTGAAGTTCGACCGCGAACGTACCCGTGTATCCCTg < 2:587540/143‑1 (MQ=255) cggcgTTGGCGGCCTGCAGCACATCACTGACATGGCCTGGGAACGCGTTAAGCATCCGAGCGAAATCGTCAACGTGGGCGACGAAATCACTGTTAAAGTGCTGAAGTTCGACCGCGAACGTACCCGTGTATCCCTGGGCCTGa < 2:517418/143‑1 (MQ=255) gcgTGGGCGGACGGCTGCACATCACTGACATGGCCTGGAAACGCGTTAAGCATCCGAGCGAAATCGTCAACGTGGGCGACGAAATCACTGTTAAAGTGCTGAAGTTCGACCGCGAACGTACCCGTGTATCCCTGGGCCTGaaa < 1:225042/143‑1 (MQ=255) ttGACGGCCTGCTGCACATCACTGACATGGCCTGGAAACGCGTTAAGCATCCGAGCGAAATCGTCAACGTGGGCGACGAAATCACTGTTAAAGTGCTGAAGTTCGACCGCGAACGTACCCGTGTCTCCCTGGGCCTGAAAccg > 2:476935/1‑141 (MQ=255) gACGGCCTGCTGCACATCACTGACATGGCCTGGAAACGCGTTAAGCATCCGAGCGAAATCGTCAACGTGGGCGACGAAATCACTGTTAAAGTGCTGAAGTTCGACCGCGAACGTACCCGTGTATCCCTGGGCCTGAAACAGCt < 2:482920/143‑1 (MQ=255) aCGGCCTGCTGCACATCACTGACATGGCCTGGGAACGCGTTAAGCATCCGAGCGAAATCGTCAACGTGGGCGACGAAATCACTGTTAAAGTGCTGAAGTTCGACCGCGAACGTACCCGTGTATCCCTGGGCCTGAAACAGCTg < 1:699333/143‑1 (MQ=255) ggCCTGCTTCACATCTCTGACATGGCCCGGAAACGCGTTAAGCATCCGAGCGAAATCGTCAACGTGGGCGACGAAATCACTGTTAAAGTGCTGAAGTTCGACCGCGAACGTACCCGTGTATCCCTGGGCCTGAAACAGCTggg < 2:820652/143‑1 (MQ=255) ggCCTGCTGCACATCACTGACATGGCCTGGGAACGCGTTAAGCATCCGAGCGAAATCGTCAACGTGGGCGACGAAATCACTGTTAAAGTGCTGAAGTTCGACCGCGAACGTACCCGTGTATCCCTGGGCCTGAAACAGCTggg > 1:765524/1‑143 (MQ=255) cccTGCTGCACATCACTCACATGCCCTGGAAACGCGTTAAGCATCCGAGCGAAATCGTCAACGTGGGCGACGAAATCACTGTTAAAGTGCTGAAGTTCGACCGCGAACGTACCCGTGTATCCCTGGGCCTGAAACAGCTGGGc < 2:819482/142‑1 (MQ=255) ctgctgCACATCACTGACATGGCCTGGAAACGCGTTAAGCATCCGAGCGAAATCGTCAACGTGGGCGACGAAATCACTGTTAAAGTGCTGAAGTTCGACCGCGAACGTACCCGTGTATCCCTGGGCCTGAAACAGCTGCGCGa > 2:252669/1‑143 (MQ=255) tgctgcACATCACTGACATGGCCTGGAAACGCGTTAAGCATCCGAGCGAAATCGTCAACGTGGGCGACGAAATCACTGTTAAAGTGCTGAAGTTCGACCGCGAACGTACCCGTGTATCCCTGGGCCTGAAACAGCTGGGCGaa < 1:494164/143‑1 (MQ=255) ctgcACATCACTGACATGGCCTGGAAACGCGTTAAGCATCCGCGCGAAATCGTCAACGTGGGCGACGAAATCACTGTTAAAGTGCTGAAGTTCGACCGCGAACGTACCCGTGTATCCCTGGGCCTGAAACAGCTGGGCGAAGa > 1:277598/1‑143 (MQ=255) ctgcACATCACTGACATGGCCTGGAAACGCGTTAAGCATCCGAGCGAAATCGTCAACGTGGGCGACGAAATCACTGTTAAAGTGCTGAAGTTCGACCGCGAACGTACCCGTGTATCCCTGGGCCTGAAACAGCTGGGCGAAGa > 1:278676/1‑143 (MQ=255) cttaTCCCTGATTTGGCCTGGAAACGCGTTAAGCATCCGAGCGAAAGCGGCAACGGGGGCGACGAAATCACTGTTAAAGTGCTGAAGTTCGACCGCGAACGTACCCGTGTATCCCTGGGCCTGAAACAGCTGGGCGAAGATcc < 1:13414/140‑1 (MQ=255) caTCACTGACATGGCCTGGAAACGCGTTAAGCATCCGAGCGAAATCGTCAACGTGGGCGACGAAATCACTGTTAAAGTGCTGAAGTTCGACCGCGAACGTACCCGTGTATCCCTGGGCCTGAAACAGCTGGGCGAAGATCCGt < 1:696197/143‑1 (MQ=255) ccccTGACATGGCCTGGAAACGCGTTAAGCATCCGAGCGAGATCGGCAACGTGGGCGACGAAATCACTGTTAAAGTGCTGAAGTTCGACCGCGAACGTACCCGTGTATCCCTGGGCCTGAAACAGCTGGGCGAAGATCCGTgg < 2:74725/140‑1 (MQ=255) ggCCTGGGAACGCGTTAAGCATCCGAGCGAAATCGTCAACGTGGGCGACGAAATCACTGTTAAAGTGCTGAAGTTCGACCGCGAACGTACCCGTGTATCCCTGGGCCTGAAACAGCTGGGCGAAGATCCGTGGGTAGCTAGCg > 2:201753/1‑143 (MQ=255) gCCTGGAAACGCGTTAAGCATCCGAGCGAAATCGTCAACGTGGGCGACGAAATCACTGTTAAAGTGCTGAAGTTCGACCGCGAACGTACCCGTGTATCCCTGGGCCTGAAACAGCTGGGCGAAGCGCCGTGGGTAGCTATCGc > 2:572962/1‑143 (MQ=255) gCCTGGAAACGCGTTAAGCATCCGAGCGAAATCGTCAACGTGGGCGACGAAATCACTGTTAAAGTGCTGAAGTTCGACCGCGAACGTACCCGTGTATCCCTGGGCCTCAAACAGCTGGGCGAAGATCCGTGGGTAGCTATCGc > 2:382088/1‑143 (MQ=255) cTGGAAACGCGTTAAGCATCCGAGCGAAATCGTCAACGTGGGCGACGAAATCACTGTTAAAGTGCTGAAGTTCGACCGCGAACGTACCCGTGTATCCCTGGGCCTGAAACAGCTGGGCGAAGATCCGTGGGTAGCTATCGCTa > 1:862349/1‑143 (MQ=255) tGGGAACGCGTTAAGCATCCGAGCGAAATCGTCAACGTGGGCGACGAAATCACTGTTAAAGTGCTGCAGTTCGACCGCGAACGTACCCGTGTATCAATGGGCCTGAAACAGCTGGGCGAAGATCCGAGGGTAGCTATCGCTaa > 2:135379/1‑143 (MQ=255) tGGAAACGCGTTAAGCATCCGAGCGAAATCGTCAACGTGGGCGACGAAATCACGGTTAAAGTGCTGAAGTTCGACCGCGAACGTACCCGTGTAGCCCTGGGCCTGAAAGAGCTGGGCGAGGAGCCGTGGGTAGCGATCGCtcc > 1:274051/1‑141 (MQ=255) | AGAGCGCGATCAGCTGCTGGAAAACCTGCAGGAAGGCATGGAAGTTAAAGGTATCGTTAAGAACCTCACTGACTACGGTGCATTCGTTGATCTGGGCGGCGTTGACGGCCTGCTGCACATCACTGACATGGCCTGGAAACGCGTTAAGCATCCGAGCGAAATCGTCAACGTGGGCGACGAAATCACTGTTAAAGTGCTGAAGTTCGACCGCGAACGTACCCGTGTATCCCTGGGCCTGAAACAGCTGGGCGAAGATCCGTGGGTAGCTATCGCTAA > NC_000913/962534‑962809 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |