| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_000913 | 1,177,647 | C→A | 10.8% | L110I (CTT→ATT) | lolE → | lipoprotein release complex ‑ inner membrane subunit |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 1,177,647 | 0 | C | A | 10.8% | 91.7 / 4.8 | 38 | L110I (CTT→ATT) | lolE | lipoprotein release complex ‑ inner membrane subunit |
| Reads supporting (aligned to +/- strand): ref base C (15/18); new base A (2/2); total (18/20) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.89e-01 | |||||||||||
ATGGTGAAATCGAGGCGGTGGATCAGCCGTGGACTAACTGGCAGGAAGCACTGGATCACGTGCAAAAAGTGCCGGGTATTGCCGCTGCCGCGCCGTATATCAATTTCACCGGGCTGGTGGAAAGTGGCGCGAATCTTCG‑CGCAATCCAGGTGAAGGGCGTTAACCCGCAACAGGAACAGCGTCTGAGCGCATTACCCTCGTTTGTCCAGGGCGATGCGTGGCGCAATTTTAAAGCGGGCGAACAGCAAATTATCATCGGCAAAGGCGTGGCGGATG > NC_000913/1177513‑1177788 | aTGGTGAAATCGAGGCGGTGGATCAGCCGTGGACTAACTGGCAGGAAGCACTGGATCACGTGCAAAAAGTGCCGGGTATTGCCGCTGCCGCGCCGTTTATCAATTTCACCGGGCTGGTGGAAAGTGGCGCGAATCTTCG‑CGCa < 1:712985/143‑1 (MQ=255) tGGTGAAATCGAGGCGGTGGATCAGCCGTGGACTAACTGGCAGGAAGCACTGGATCACGTGCAAAAAGTGCCGGGTATTGCCGCTGCCGCGCCGTATATCAATTTCACCGGGCTGGTGGAAAGTGGCGCGAATCTTCG‑CGCaa < 1:853354/143‑1 (MQ=255) tGGTGAAATCGAGGCGGTGGATCAGCCGTGGACTAACTGGCAGGAAGCACTGGATCACGTGCAAAAAGTGCCGGGTATTGCCGCTGCCGCGCCGTATATCAATTTCACCGGGCTGGTGGAAAGTGGCGCGAAGATTCg‑cgcca < 1:184593/143‑3 (MQ=255) tGGGGAAATCGAGGCGGCTGATCAGCCGTGGACTGACTGGCAGGAAGCACTGGATCACGTGCAAAAAGTGCCGGGTATTGCCGCTGCCGCGCCGTATATCAATTTCACCGGGCTGGTGGAAAGTGGCGCGAAGATTCg‑cgcca < 2:797550/143‑3 (MQ=255) ggTGAAAGGGAGGAGGTGGCTCAACCGGCGACGATCTGGCAGGAAGCACTGGTTCACGTGCAAAAAGTTCCGGGTATTGCCGGTGCCGCGCCGTATATCAATTTCACCGGGCTGGTGGAAAGTGGCGCGAATCTTCG‑CGCAAt < 2:320841/143‑1 (MQ=255) cgAAATCGAGGCGGTGGATCAGCCGTGGACTAACTGGCAGGAAGCAATGGCCCACGTGCAAAAAGTGCCGGGTATTGCCGCTGCCGCGCCGTATATCAATTTCACCGGGCTGGTGGAAAGTGGCGCGAATCTTCG‑CGCAATcc < 1:287265/142‑1 (MQ=255) gAAATCGAGGCGGTCGAGCATCCGGGCGCTAACTGGCAGGAAGCACTTGATCACGTGCAAAAAGTGCCGGGCATTGCCGCTGCCGCGCCGTATATCAATTTCACCGGGCTGGTGGAAAGTGGCGCGAATCTTCG‑CGCAATCCa < 1:844726/143‑1 (MQ=255) aaTCGAGGCGGTGGATCAGCCGTGGACTAACTGGCAGGAAGCACTGGATCACGTGCAAAAAGTGCCGGGTATTGCCGCTGCCGCGCCGTATATCAATTTCACCGGGATGGTGGAAAGTGGCGCGCATCTTCG‑CGCAAGccggc > 2:836564/1‑140 (MQ=255) aGGCGGTGGATCAGCCGTGGACTAACTGGCAGGAAGCACTGGATCACGTGCAAAAAGTGCCGGGTATTGCCGCTGCCGCGCCGTATCTAAATTTCACCGGGCTGGTGGAAAGTGGCGCGAATCTTCG‑CGCAATCAAGGTGAAg > 1:151397/1‑143 (MQ=255) gCGGTGGATCAGCCGTTGACTACCTGGCAGGGAGCACTGGATCACGTGCAAAACGTGCCGGGTATTGCCGCTGCCGCGCCGTATATCAATTTCACCGGGCTGGTGGAAAGTGGCGCGAATCTTCG‑CGCAATCCAGGTGAAggg < 1:321519/143‑1 (MQ=255) tAACTGGCAGGAAGCACTGGATCACGTGCAAAAAGTGCCGGGTATTGCCGCTGCCGCGCCGTATATCAATTTCACCGGGCTGGTGGAAAGTGGCGCGAATCTTCG‑CGCAATCCAGGTGAAGGGCGTTAACCCGCAACAGGAAc > 1:119794/1‑143 (MQ=255) gcaCTGGCGGGAGGCGCTGTATCACGTGCAAAAAGTCCCGGGTAGTGCCGCTGCCGCGCCGTATATCACTTTCACCGGGCTGGTGGAAAGTGGCGCGAATCTTCG‑CGCAATCCAGGTGAAGGGCGTTAACCCGCAACAGGAAc < 2:668239/141‑1 (MQ=255) cTGGCAGGAGGCACTGGATCACGTGCCAAAAGTGCCGGGTAGTGCCGCTGCCGCGCCGTATATCAATTGCACCGGGGTGGTGGAAAGTGGCGCGAATCTTCG‑CGCAATCCAGGTGAAGGGCGTTAACCCGCAACAGGAACAGc < 2:809601/143‑1 (MQ=255) cTGGCAGGAAGCACTGGATCACGTGCAAAAAGTGCCGGGTATTGCCGCTGCCGCGCCGTATATCAATTTCACCGGGCTGGTGGAAAGTGGCGCGAATCTTCG‑CGCAATCCAGGTGAAGGGCGTTAACCCGCAACAGGAACAGc < 1:383307/143‑1 (MQ=255) cTGGCAGGAAGCACTGGATCACGTGCAAAAAGTGCCGGGTATAGCCGCTGCCTCGCCCTCAATCAATTTCACCGGGCTGGTGGAAAGTGCCGCGTATGTGCC‑CGCAATCACGGGGAAGGGCGTGAACCCGCAACAGGAACCGc > 2:823341/1‑143 (MQ=255) tGGATCACGTGCAAAAAGTGCCGGGTATTGCCGCTGCCGCGCCGTATATCAATTTCACCGGGCTGGTGGAAAGTGGCGCGAATCTTCG‑CGCAATCCAGGTGAAGGGCGTTAACCCGCAACAGGAACAGCGTCTGAGCGCATTa > 1:549063/1‑143 (MQ=255) cACGTGCAAAAAGTGGCGGGTGTTGCCGCTGCCGCGCGGGATATCAAGTTCACCGGGCTGGTGGAAAGTGGCGCGAATCTTCG‑CGCAATCCAGGTGAAGGGCGTTAACCCGCAACAGGAACAGCGTCTGAGCGCATTACCCTc < 1:473809/143‑1 (MQ=255) agggccAAAACGGGCCGGGTGGTGCCGGGGGCGCGCCGGAGATCAAGTTCACCGGGCTGGTGGAAAGTGGCGCGAATCTTCG‑CGCAATCCAGGTGAAGGGCGTTAACCCGCAACAGGAACAGCGTCTGAGCGCATTACCCTCg < 2:612955/138‑1 (MQ=255) cAAAAAGTGCCGGGTATTGCCGCTGCCGCGCCGTATATCAATTTCACCGGGCTGGTGGCAAGAGGCGGGAATCTTCG‑CGCAATCCAGGTGAAGGGCGTTAACCCGCAACAGGAACAGCGTCTGAGCGCATTACCCTCGTTTGt > 1:802629/1‑143 (MQ=255) cGGGTATTGCCGCTGCCGCGCCGTATATCAATTTCACCGGGCTGGTGGAAACTGGCGCGAATCTTCG‑CGCACTCCAGGTGAAGGGCGTTAACCCGCAACAGGAACAGCGTCTGAGCGCATTACCCTCGTTTGTCCAGGGCGAt > 1:829585/1‑143 (MQ=255) ggTATTGCGGCTGCCGGGCCGTATAGCAATTTGACCGGGCTGGTGGACAGTGGCGCGCATCTTCG‑CGCAATCCAGGTGAAGGGCGTGAACCCGCAACAGGGACAGCGTCTGAGCGCATTACCCTTGTTTGTCCAGGGCGATGc < 2:457030/143‑1 (MQ=255) ggTATTGCCGCTGCCGCGCCGTATATCAATTTCACCGGGCTGGTGGAAAGTGGCGCGAATCTTCG‑CGCAATCCAGGTGAAGGGCGTTAACCCGCAACAGGAACAGCGTCTGAGCGCATTACCCTCGTTTGTCCAGGGCGATGc > 2:710436/1‑143 (MQ=255) tttGCCGCTGCCGCGCCGGATATCGATTGCGCCGGGCTGGTGGAAAGTGGCGCGAATCTTCG‑CGCAATCCAGGTGAAGGGCGTTAGCCCGCAACAGGAACAGCGTCTGAGCGCATTACCCTCGTTTGTCCAGGGCGATGCGTg < 2:203541/142‑1 (MQ=255) gccgctgccgcGCCGGATATCAATTTCACCGGGCTGGTGGACAGTGGCGCGAATCGTCG‑CGCAATCGAGGTGAAGCGCGTTAACGCGCAACAGGAACAGCGTCTGAGCGCATTACCCTCGTGTGTCCAGGGCGATGCGTGgcg > 2:754598/1‑143 (MQ=255) ctgccgcGCCGTATATCAATTTCACCGGGCTGGTGGAAAGTGGCGCGAATCTTCG‑CGCAAGCCAGGTGAAGGGCGTTAACCCGCAACAGGAACAGCGTCTGAGCGCATTACCCTCGTTTGTCCAGGGCGATGCGTGGCGCAAt < 1:424731/143‑1 (MQ=255) cggccgcGCCGTATATCAATTTCACCGGGCTGGTGGAAAGGGGCGCGAATCTTCG‑CGCAATCCAGGTGAAGGGCGTTAACCCGCAACAGGAACAGCGTCTGAGCGCATTACCCTCGTTTGTCCAGGGCGATGCGTGGCGCAAt < 1:548640/141‑1 (MQ=255) ccgcGCCGTATATCAATTTCACCGGGCTGGTGGAAAGTGGCGCGAATCTTCG‑CGCAATCCAGGTGAAGGGCGTTAACCCGCAACAGGAACAGCGTCTGAGCGCATTACCCTCGTTTGTCCCGGGCGATGGGTGGCGCAActtt > 2:557370/1‑143 (MQ=255) cgcCGTATATCAATTTCACCGGGCTGGTGGAAAGTGGCGCGAATCTTCG‑CGCAATCCAGGTGAAGGGCGTTAACCCGCAACAGGAACAGCGTCTGAGCGCATTACCCTCGTTTGTCCAGGGCGATGCGTGGCGCAATTTTaaa < 1:759966/143‑1 (MQ=255) tCAATTTCACCGGGCTGGTGGAAAGTGGCGCGAATCTTCG‑CGCAATCCAGGTGAAGGGCGTTAACCCGCAACAGGAACAGCGTCTGAGCGCATTACCCTCGTTTGTCCAGGGCGATGCGTGGCGCAATTTTAAAGCGGGCGaa < 1:678248/143‑1 (MQ=255) tCAATTTCACCGGGCTGGTGGAAAGTGGCGCGAATCTTCG‑CGCAATCCAGGTGAAGGGCGTTAACCCGCAACAGGAACAGCGTCTGAGCGCATTACCCTCGTTTGTCCAGGGCGATGCGTGGCGCAATTTTAAAGCGGGCGaa > 1:501238/1‑143 (MQ=255) tttCACCGGGCTGGTGGAAAGTGGCGCGAATCTTCG‑CGCAATCCAGGTGAAGGGCGTTAACCCGCAACAGGAACAGCGTCTGAGCGCATTACCCGCGTTTGTCCAGGGCGATGCGTGGCGCAATTGTAAAGCGGGCGAACAGc > 1:36854/1‑143 (MQ=255) aCCGGGCTGGTGGAAAGTGGCGCGAATCTTCG‑CGCAATCCAGGTGAAGGGCGTTAACCCGCAACAGGAACAGCGTCTGAGCGCATTACCCTCGTTTGTCCAGGGCGATGCGTGGCGCAATCTAAAATCGGGCGAACAGCaaag > 2:215179/1‑142 (MQ=255) cGGGCTGGTGGAAAGTGGCGCGAATCTTCG‑CGCAATCCAGGTGAAGGGCGTTAACCCGCAACCGGAACAGCGTCTGAGCGCATTACCCTCGTTTGTCCAGGGCGATGCGTGGCGCCATTTTAAAGCGGGCGAACCGCAACTTa > 1:343512/1‑143 (MQ=255) ggtggAAAGTGGCG‑AAAACTTCGCCTCAATCCAGGTGAACGGCGTTAACCCGCAACAGGAACAGCGTCTGAGCGCATTACCCTCGTTTGTCCAGGGCGATGCGTGGCGCAATTTTAAAGCGGGCGAACAGCAAATTATCATCg < 2:5098/143‑1 (MQ=255) ggAAAGTGGCGCGAATCTTCG‑CGCAATCCAGGTGAAGGGCGTTAACCAGCAACAGGAACAGCGTCTGAGCGCATTACCCTCGTTTGTCCAGGGCGATGCGTGGCGCAATTTTAAAGCGAGCGAACAGCAAATTATCATCGGCa > 1:567018/1‑143 (MQ=255) ctggattgcgcGAAGATTCG‑CGCAATCCAGGTGAAGGGCGTTAACCCGCAACAGGAACAGCGTCTGAGCGCATTACCCTCGTTTGTCCAGGGCGATGCGTGGCGCAATTTTAAAGCGGGCGAACAGCAAGTTATCCTCGGCaa > 1:45651/8‑143 (MQ=255) gattgcgcGAAGATTCG‑CGCAATCCAGGTGAAGGGCGTTAACCCGCAACAGGAACAGCGTCTGAGCGCATTACCCTCGTTTGTCCAGGGCGATGCGTGGCGCAATTTTAAAGCGGGCGCACAGCCAATTATCATCGGCAAAgg > 2:824246/5‑143 (MQ=255) agcTTCG‑CGCAATCCAGGTGAAGGGCGTTAACCCGCAACAGGAACAGCGTCTGAGCGCATTACCCTCGTTTGTCCAGGGCGATGCGTGGCGCAATTTTAAAGCGGGCGAACAGCAAATTATCATCGGCAAAGGCGTGGCGGAt < 1:132865/141‑1 (MQ=255) tCTTCG‑CGCAATCCAGGTGAAGGGCGTTAACCCGCAACAGGAACAGCGTCTGAGCGCATTACCCTCGTTTGTCCAGGGCGATGCGTGGCGCAATTTTAAAGCGGGCGAACAGCAAATTATCATCGGCAAAGGCGTGGGGGATg > 1:478723/1‑143 (MQ=255) | ATGGTGAAATCGAGGCGGTGGATCAGCCGTGGACTAACTGGCAGGAAGCACTGGATCACGTGCAAAAAGTGCCGGGTATTGCCGCTGCCGCGCCGTATATCAATTTCACCGGGCTGGTGGAAAGTGGCGCGAATCTTCG‑CGCAATCCAGGTGAAGGGCGTTAACCCGCAACAGGAACAGCGTCTGAGCGCATTACCCTCGTTTGTCCAGGGCGATGCGTGGCGCAATTTTAAAGCGGGCGAACAGCAAATTATCATCGGCAAAGGCGTGGCGGATG > NC_000913/1177513‑1177788 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |