Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,132,787 A→C 100% I204S (ATC→AGC)  wcaA ← putative colanic acid biosynthesis glycosyl transferase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,132,7870AC100.0% 86.6 / NA 33I204S (ATC→AGC) wcaAputative colanic acid biosynthesis glycosyl transferase
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base C (18/15);  total (18/15)
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

TGGTAGAGGGTAAACAGCTGATATTTTTTGCTGGCGCGGTCGAATTTGTCTTTGTGCTTGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGATCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGAAGATGTCGTAATCCTGTGCCGCTTTCAGTTCGGTATCGAACAAACACTCTTTGAAACGCCATGCCCAGGTAAAGACCTGG  >  NC_000913/2132652‑2132924
                                                                                                                                       |                                                                                                                                         
tGGTAGAGGGTAAACAGCTGATATTTTTTGCTGGCGCGGTCGAATTTGTCTTTGTGCTTGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGGAGTGGATATGCAGGCTCTGCGt                                                                                                                                    >  1:484623/1‑143 (MQ=255)
  gTAGAGGGTAAACAGCTGATATTTTTTGCTGGCGCGGTCGAATTTGTCTTTGTGCTTGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCg                                                                                                                                  <  1:443897/143‑1 (MQ=255)
   tAGAGGGTAAACAGCTGATATTTTTTGCTGGCGCGGTCGAATTTGTCTTTGTGCTTGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGc                                                                                                                                 <  2:250326/143‑1 (MQ=255)
      agGGTAAACAGCTGTTGTGTTTTGCTGGCGCTGTCGAATTTGTCTTTGTGCTTGCTGTAAAAGTTGAAGTACCCGGAGAATTTTTTCGGTGACTAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCctc                                                                                                                              <  2:662294/143‑1 (MQ=255)
        ggTACACAGCGGATATTTCTTGCTGGCGCGGTCGAATTTGTCTTTGTGCTTGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCtctt                                                                                                                            <  1:265680/143‑1 (MQ=255)
        ggTAAACAGCTGATATTTTTTGCTGGCGCGGTCGACTTTGTCTTTGTGCTTGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGCGATCGATATGCAGGCGCTGCGCCGCctctg                                                                                                                            >  2:789754/1‑142 (MQ=255)
              cAGCTGATATTTTTTGCTGGCGCGGTCGAATTTGTCTTTGTGCTTGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACtt                                                                                                                      >  2:280690/1‑143 (MQ=255)
              cAGCTGATATTTTTTGCTGGCGCGGTCGAATTTGTCTTTGTGCTTGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACtt                                                                                                                      >  2:734249/1‑143 (MQ=255)
                ggttatatTTTTTGCTGGCGCGGTCGAATTTGTTTTTGTGCTTGCGGTAAAAGTGGAAGTACCCGGAGAATGTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTc                                                                                                                    <  2:277182/139‑1 (MQ=255)
                 cTGATATTTTTTGCTGGCGCGGTCGAATTTGTCTTTGTGCTTGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTcc                                                                                                                   >  2:752890/1‑143 (MQ=255)
                  tGATATTTTTTGTTGGCGCGGTAGAATTTGTCTTTGTGCTTGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGAGATTGATATGCAGGCTCTGCGTAGCCTCTTCTACTTTCCa                                                                                                                  >  2:800735/1‑143 (MQ=255)
                  tGATATTTTTTGCTGGCGCGGTCGAATTTGTCTTTGTGCTTGCGGTAAAAGTGGACGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTCCTTTCCa                                                                                                                  >  1:322672/1‑143 (MQ=255)
                       ttttttGCTGGCGCGGTCGAATTTGTCTTTGTGCTTGCTGTAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGCTTGATATGCAGGCTCTGCGCCGCCCCTTTTCCTTTCCACggct                                                                                                             >  2:541974/1‑141 (MQ=255)
                       ttgtttGTTGGCGCGGTCGATTTTGTCTTTGTGCGTGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGtt                                                                                                             <  2:727514/140‑1 (MQ=255)
                          tttGCTGGCGCGGTCGAATTTGTCTTTGTGCTTGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGc                                                                                                          >  1:570655/1‑143 (MQ=255)
                               tGGCGCGGTCGAATTTGTCTTTGTGCTTGCGGTAAAAGTGGAAGTTCCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTAc                                                                                                     <  1:519259/143‑1 (MQ=255)
                               tGGCGCGGTCGAATTTGTCTTTGTGCTTGCGGTAAAAGTGGAAGTACACGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCGCTGCGTCGCCTGCTCTACTTTCCACGGATCGCCGtcc                                                                                                     >  2:896265/1‑141 (MQ=255)
                                                tCTTTGTGCTTGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTGCTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAg                                                                                    >  1:60454/1‑143 (MQ=255)
                                                     gtgCTTGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCAGCCGCAGGAAgg                                                                               >  1:281437/1‑142 (MQ=255)
                                                         ttGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGAAGATGTc                                                                           >  2:610485/1‑143 (MQ=255)
                                                                    gcggAAGCACCCGACGACGTTCTTCGGTGACGAGGTGATCTGCATTTCGCCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGAAGATGTCGTAATCCtgtg                                                                <  2:528668/141‑1 (MQ=255)
                                                                          gtgcccGGAGAATTTGTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGAAGATGTCGTAATCCTGTGCCGCtt                                                          <  2:233160/140‑1 (MQ=255)
                                                                             cccGGAGAATTTTTTCGGGGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGAAGATGTCGTAATCCTGTGCCGCTTTCa                                                       <  1:77879/143‑1 (MQ=255)
                                                                                 gagaATTTTTTCGGTGACGGGGTGCTCTGCATTTCGCCGTGATTGATATGCAGGCTCTGCGTCTCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGAAGATGTCGTAATCCTGTGCCGCTTTCAGTTc                                                   <  2:688309/143‑1 (MQ=255)
                                                                                      ttttttCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGGCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGAAGATGTCGTAATCCTGTGCCGCTTTCAGTTCGGTac                                              >  2:539312/1‑142 (MQ=255)
                                                                                          ttCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTCACACCGTTCGCCGTACTCCACCACCATCCGCAGGAAGATGTCGTAATCCTGTGCCGCTTTCAGTTCGGTATCGaa                                          <  1:561016/143‑1 (MQ=255)
                                                                                            cGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCAGCGTCGCCTCTTCTACTTTCCACGATTCGCCGTACTCCACCACAAAGCCCAGGAAGATGTAGTAAGGCTGTGACACTATCAAATAGGGAGCGaaca                                        >  2:582434/1‑143 (MQ=255)
                                                                                              gTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGAAGATGTCGGAATCCTGTGCCGCTTTCAGTGCGGTATCGagcaaa                                      >  1:305619/1‑143 (MQ=255)
                                                                                                  cGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTGCCACGGTTCGCCGTACTCCACCACCATCCGCAGGAAGATGTCGTAATCCTGTGCCGCTTTCAGTTCGGTATCGAACAAACAct                                  <  2:46785/143‑1 (MQ=255)
                                                                                                        gATCTGCATTGCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGAAGATGTCGTAATCCTGTGCCGCTTTCAGTTCGGTATCGAACAAACACTCTTTGa                            <  1:869304/143‑1 (MQ=255)
                                                                                                            tGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGAAGATGTCGTAATCCTGTGCCGCTTTCAGTTCGGTATCGAACAAACGCTCTTTGAAACg                        >  2:80261/1‑143 (MQ=255)
                                                                                                               aTTTCACCGTGGTTGATACGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGAAGATGTCGTAATCCTGTGCCGCTTTCAGTTCGGTATCGAACAAACACTCTTTGAAACGCCa                     <  2:888864/143‑1 (MQ=255)
                                                                                                                                  gCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACAACCATCCGCAGGAAGATGTCGTAATCCTGTGCCGCTTTCAGTTCGGTATCGAACAAACACTCTTTGAAACGCCATGCGCAGGTAAAGACCTgg  >  2:417844/1‑143 (MQ=255)
                                                                                                                                       |                                                                                                                                         
TGGTAGAGGGTAAACAGCTGATATTTTTTGCTGGCGCGGTCGAATTTGTCTTTGTGCTTGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGATCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGAAGATGTCGTAATCCTGTGCCGCTTTCAGTTCGGTATCGAACAAACACTCTTTGAAACGCCATGCCCAGGTAAAGACCTGG  >  NC_000913/2132652‑2132924

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑