| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_000913 | 2,575,770 | G→A | 10.0% | P12P (CCC→CCT) | eutS ← | putative structural protein, ethanolamine utilization microcompartment |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 2,575,770 | 0 | G | A | 10.0% | 134.7 / 2.3 | 56 | P12P (CCC→CCT) | eutS | putative structural protein, ethanolamine utilization microcompartment |
| Reads supporting (aligned to +/- strand): ref base G (23/27); new base A (3/3); total (26/30) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
CGCTAAATCACCGGCGATCATCGCCGTTTCGCCGGGAGTTAGCGTCATAATGCCAATTGCGCCCGCATCCGGAACGCCGATTTTCTTCGCCAGTTCCTCACCGGGATGCGCGATGAGATGCGCCAGTGTGACCTGTTTGCCGGGCACAAATTCCTGAATGATGCGTTCTTTATCCATGAGTCGCCGCCTGTGAAAAACTTTCGTGTATCGTGACAATTTTTCGTCAGAGTAAATAACAAAATTCGGCAAGCGCGTTTAAAAGGTGAGGTAGATCACTAAGAATG > NC_000913/2575629‑2575912 | cgcTAAACCCCCGGCGATCATCGCCGTTTCGCCGGGAGTTAGCGTCATAATGCCAATTGCGCCCGCATCCGGAACGCCGATTTTCTTCGCCAGTTCCCCACCGGGATGCGCGATGAGATGCGCCAGTGTGACCTGTTTGCCgg < 2:810468/143‑1 (MQ=255) aaTCACCGGCGATCATCGCCGTTTCGCCGGGAGTTAGCGTCATAATGCCAATTGCGCCCGCATCCGGAACGCCGATTTTCTTCGCCAGTTCCTCACCGGGATGCGCGCTGAGATGCGCCAGTGTGACCTGTTTGCCGGGcaca > 2:244914/1‑143 (MQ=255) ccGGCGATCAGCGCCGTGTCGCCGGGAGTTAGGGTCAGAAGTCCACTTGCGCCCGCATCCGGAACGCCGATTTTCTTCGCCAGTTCCTCACCGGGATGCGCGATGAGATGCGCCAGTGTGACCTGTTTGCCGGGCACAAATTc < 2:866106/143‑1 (MQ=255) ggCGCTCATCGCCGGTTCGCCGGGAGTTAGCGTCATAATGCCAATTGCGCCCGCATCCGGAACGCCGATTTTCTTCGCCAGTTCCTCACCGGGATGCGCGATGAGATGCGCCAGTGTGACCTGTTTGCCGGGCACAAATTCCt < 2:163844/143‑1 (MQ=255) ggCGATCATCGCCGTTTCGCCGGGAGTTAGCGTCATAATGCCAATTGCGCCCGCATCCGGAACGCCGATTTTCTTCGCCAGTTCCTCACCGGGATGCGCGATGAGATGCGCCAGTGTGACCTGTTTGCCGGGCACAAATTCCt < 1:189249/143‑1 (MQ=255) cGATCATCGCCGTTTCGCCGGGAGTTAGCGTCATAATGCCAATTGCGCCCGCATCCGGAACGCCGATTTTCTTCGCCAGTTCCTCACCGGGATGCGCGATGAGATGCGCCAGTGTGACCTGTTTGCCGGGCACAAATTCCTGa < 1:3960/143‑1 (MQ=255) gATCATCGCCGTTTCGCCGGGAGTTAGCGTCATAATGCCAATTGCGCCCGCATCCGGAACGCCGATTTTCTTCGCCAGTTCCTCACCGGGATGCGCGATGAGATGCGCCAGTGTGACCTGTTTGCCGGGCACAAATTCCTGaa < 1:377325/143‑1 (MQ=255) tcGCCGTTTCGCCGGGAGTTAGCGTTGTATTGCCAATTGCGCCCGCATCCGGAACGCCGATTTTCTTCGCCAGTTCCTCACCGGGATGCGCGATGAGATGCGCCAGTGTGACCTGTTTGCCGGGCACAAATTCCTGAatgatg > 2:543533/1‑143 (MQ=255) ccGGGAGTTAGCGTCATTATGCCAATTGCGTCCGCATCCGGAACGCCGATTTTCTTCTCCAGTTCCTCACCGGGATGCGCGATGAGATGCGCCAGTGTGACCTGTTTGCCGGGCACAAATTCCTGAATGATGCGTTCTTTATc < 2:674988/143‑1 (MQ=255) ccGGGAGTTAGCGTCATAATGCCAATTGCGCCCGCATCCGGAACGCCGATTTTCTTCGCCAGTTCCTCACCGGGATGCGCGATGAGATGCGCCAGTGTGACCTGTTTGCCGGGCACAAATTCCTGAATGTAGCCTTCTTTATc > 2:825475/1‑143 (MQ=255) ccGGGAGTTAGCGTCATAATGCCAATTGCGCCCGCATCCGGAACGCCGATTTTCTTCGCCAGTTCCTCACCGGGATGCGCGATGAGATGCGCCAGTGTGACCTGTTTGCCGGGCACAAATTCCTGAATGATGCGTTCTTTATc > 2:585684/1‑143 (MQ=255) gggAGTTAGCGTCATAATGCCAATTGCGCCCGCATCCGGAACGCCGATTTTCTTCGCCAGTTCCTCACCGGGATGCTCGATGAGATGCGACAGCACCCAGTGGTTGCTAGGAAAGAATTCCAGAATGATGCGTTCTTTATCCa > 2:308083/1‑143 (MQ=255) gggAGTTAGCGTCATAATGCCAATTGCGCCCGCATCCGGAACGCCGATTTTCTTCGCCAGTTCCTCACCGGGATGCGCGATGAGATGCGCCAGTGTGACCTGTTTGCCGGGCACAAATTCCTGAATGATGCGTTCTTTATCCa > 1:379689/1‑143 (MQ=255) gggAGTTAGCGTCATAATGCCAATTGCGCCCGCATCCGGAACGCCGATTTTCTTCGCCAGTTCCTCACCGGGATGCGCGATGAGATGCGCCAGTGTGACCTGTTTGCCAGGCACAAATTCCTGAATGATGCGTTCTTTATCCa > 2:773362/1‑143 (MQ=255) gggAGTTAGCGTCATAATGCCAATTGCGCCCGCATCCGGAACGCCGATTTTCTTCGCCAGTTCCTCACCGGGATGCGCGATGAGATGCGCCAGTGTGACCTGTTTGCCAGGCACAAAGTCCTGAAGGATGCGTTCATTATCCa > 2:306961/1‑143 (MQ=255) ggcgTTGGCATCATAATGCCAATCGCGCCCGCACTCGGGACGCCGATTTTCTTCGCCAGTTCCTCACCGGGATGCGCGCTTAGATGCGCCAGTGTGACCTGTTTGCCGGGCACAAATTCCTGAATGATGCGTTCTTTATCCAt < 2:896356/140‑1 (MQ=255) cgtcgtAATGCGAATTGCGCGCGCATCCGGAACGCCTATTTTCTTCGCTAGTGCCTCACCGGGATGCGCGATGATATGCGCCAGTGTGACCTGTTTGCCGGGCACAAATTCCTGAATGATGCGTTCTTTATCCATGAGTcgcc < 2:619922/143‑1 (MQ=255) gTCATAATGCCAATTGCGCCCGCAGCCGGAACGCCGATTTTCTTCGCCAGTTCCTCACCGGGATGCGCGATGAGATGCGCCAGTGTGACCTGTTTGCCGGGCACAAATTCCTGAATGATGCGTTCTTTATCCATGAGTcgccg < 2:891775/143‑1 (MQ=255) tCATAATGCCAATTGCGCCCGCATCCGGAACGCCGATTTTCTTCGCCAGTTCCTCACCGGGATGCGCGATGAGATGCGCCAGTGTGACCTGTTTGCCGGGCACACATTCCTGAATGATGTGTTCTTTCTCCATGAGTctgcgc > 1:437353/1‑138 (MQ=255) gCCAATTGCGCCCGCATCCGGAACGCCGATTTTCTTCGCCAGTTCCTCCCCGGGACGCGCGACTAGATGCGCCAGTGTGCCCTGTTTGCCGGGCACAAATTCCTGACTGATGCGCTCTTTAGCCATCGCTCGCCGCCtgtgca > 1:34333/1‑141 (MQ=255) gCCAATTGCGCCCGCATCCGGAACGCCGATTTTCTTCGCCAGTTCCTCACCGGGAGGCGCGATGAGATGCGCCAGTGTGACCTGTTTGCCGGGCACAAATTCCTGAATGATGCGTTCTTTATCCATGAGTCGCCGCCTGTGaa > 1:295145/1‑143 (MQ=255) ccAATTGCGCCCGCATCCGGAACGCCGATTTTCTTCGCCAGTTCCTCACCGGGATGCGCGATGAGATGCGCCAGTGTGACCTGTTTGCCGGGCACAAATTCCTGAATGATGCGTTCTTTATCCATGAGTCGCCGCCTGTGaaa < 1:50246/143‑1 (MQ=255) ccAATTGCGCCCGCATCCGGAACGCCGATTTTCTTCGCCAGTTCCTCACCGGGATGCGCGATGAGATGCGCCAGTGTGACCTGTTTGCCGGGCACAAATTCATGAATGATGCGTTCTTTATCCATGAGTCGCCGCCCGTGaaa > 2:478347/1‑143 (MQ=255) aaTTGCGCCCGCATCCGGAACGCCGATTTTCTTCGCCAGTTCCTCACCGGGATGCGCGATTAGATGCGCCAGTGTGACCTGTTTGCCGGGCACAACTTCCTGAATGATGCGTTCTTTATCCATGAGTCGCCGCCTGTGaaaaa > 1:457600/1‑143 (MQ=255) tGCGCCCGCATCCGGAACGCCGATTTTCTTCGCCAGTTCCTCACCGGGATGCGCGATGAGATGCGCCAGTGTGACCTGTTTGCCGTGCACAAATTCCTGAATGATGCGTTCTTTATCCATGAGTCGCCGCCTGTGAAAAACtt > 1:183820/1‑143 (MQ=255) tGCGCCCGCATCCGGAACGCCGATTTTCTTCGCCAGTTCCTCACCGGGATGCGCGATGAGATGCGCCAGTGTGACCTGTTTGCCGGGCACAAATTCCTGAATGATGCGTTCTTTATCCATGAGTCGCCGCCTGTGAAAAGCtt > 1:255562/1‑143 (MQ=255) cgcCCGCATCCGGAACGCCGATTTTCTTCGCCAGTTCCTCACCGGGATGCGCGATGAGATGCGCCAGTGTGACCTGTTTGCCGGGCACAAATTCCTGAATGATGCGTTCTTTATCCATGAGTCGCCGCCTGTGAAAAACTTTc < 1:630759/143‑1 (MQ=255) gcCCGCATCCGGAACGCCGATTTTCTTCGCCAGTTCCTCACCGGGATGCGCGATGAGATGCGCCAGTGTGACCTGTTTGCCGGGCACAAATTCCTGAATGATGCGTTCTTTATCCATGAGTGGCCGCCTGTGAAACACTTTCg > 1:821559/1‑143 (MQ=255) ccGGAACGCCGATTTTCTTCGCCAGTTCCTCACCGGGATGCGCGATGAGATGCGCCAGTGTGACCTGTTTGCCGGGCACAAATTCCTGAATGATGCGATCTTTATCCATGACTCCCCGCCTGTGAACAACTTTCGTGTCTCGt > 1:115996/1‑143 (MQ=255) ttcGCCAGTTCCTCACCGGGATGCGCGATGAGATGCGCCAGTGTGACCTGTTTGCCGGGCACAAATTCCTGAATGATGCGTTCTTTATCCATGAGTCGCCGCCTGTGAAAAACTTTCGTGTATCGTGACAATTTTTCGTCaga < 2:426225/143‑1 (MQ=255) gtcGCCAGTTCCTCACCGGGATGCGCGATGAGATGCGCCAGTGTGACCTGTTTGCCGGGCACAAATTCCTGAATGTTGCGTTCTTTATCCATGAGTCGCCGCCTGTGAAAAACTTTCGTGTATCGTGACAATTTTTCGTCaga < 2:21152/142‑1 (MQ=255) ccAGTTCCTCACCGGGATGCGCGATGAGATGCGCCAGTGTGACATGTTTGCCGGGCACAAATTCCTGAATGATGCGTTCTTTATCCATGAGTCGCCGCCTGTGAAAAACTTTCGTGTATCGTGACAATTTTTCGTCAGAGTaa < 2:800943/143‑1 (MQ=255) ttCCTCACCGGGATGCGCGATGAGATGCGCCAGTGTGACCTGTTTGCCGGGCACAAATTCCTGAATGATGCGTTCTTTATCCATGAGTCGCCGCCTGTGAAAAACTTTCGTGTATCGTGACAATTTTTCGTCAGAGTAAATaa > 1:821219/1‑143 (MQ=255) tgccacACCGGGATGCGCGATGAGATGCGCCAGTGTGACCTGTTTGCCGGGCACAAATTCCTGAATGATGCGTTCTTTATCCATGAGTCGCCGCCTGTGAAAAACTTTCGTGTATCGTGACAATTTTTCGTCAGAGTAAATaa < 1:819572/138‑1 (MQ=255) tCACCGGGATGCGCGATGAGATGCGCCAGTGTGACCTGTTTGCCGGGCACAAATTCCTGAATGATGCGTTCTTTATCCATGAGTCGCCGCCTGTGAAAAACTTTCGTGTATCGTGACAATTTTTCGTCAGAGTAAATAACaaa > 2:55998/1‑143 (MQ=255) aCCGGGATGCGCGATGAGATGCGCCAGTGTGACCTGTTTGCCGGGCACAAATTCCTGAATGATGCGTTCTTTATCCATGAGTCGCCGCCTGTGAAAAACTTTCGTGTATCGTGACACTTTTTCGTCAGAGTAAATAACAAAAt > 2:889316/1‑143 (MQ=255) ccGGGATGCGCGATGAGATGCGCCAGTGTGACCTGTTTGCCGGGCACAAATTCCTGAATGATGCGTTCTTTATCCATGAGTCGCCGCCTGTGAAAAACTTTCGTGTATCGTGACAATTTTTCGTCAGAGTAAATAACAAAAtt < 1:723339/143‑1 (MQ=255) ccGGGATGCGCGATGAGATGCGCCAGTGTGACCTGTTTGCCGGGCACAAATTCCTGAATGATGCGTTCTTTATCCATGAGTCGCCGCCTGTGAAAAACTTTCGTGTATCGTGACAATTTTTCGTCAGAGTAAATAACAAAAtt > 2:476230/1‑143 (MQ=255) ctggATGCGCGATGAGATGCGCCAGTGTGACCTGTTTGCCGGGCACAAATTCCTGAATGATGCGTTCTTTATCCATGAGTCGCCGCCTGTGAAAAACTTTCGTGTATCGTGACAATTTTTCGTCAGAGTAAATAACAAAATTc < 1:694738/141‑1 (MQ=255) cGGGATGCGCGATGAGATGCGCCAGTGTGACCTGTTTGCCGGGCACAAATTCCTGAATGATGCGTTCTTTATCCATGAGTCGCCGCCTGTGAAAAACTTTCGTGTATCGTGACAATTTTTCGTCAGAGTAAATAACAAAAtcc > 2:551763/1‑141 (MQ=255) gggATGCGCGATGAGATGCGTCAGTGTGACCTGTTTGCCGGGCACAAATTCCTGAATGATGCGTTCTTTATCCATGAGTCGCCGCCTGTGAAAAACTTTCGTGTATCGTGACAATTTTTCGTCAGAGTAAATAACAAAATTCg < 1:772652/143‑1 (MQ=255) ggATGCGCGATGAGATGCGCCAGTGTGACCTGTTTGCCGGGCACAAATTCCTGAATGATGCGTTCTTTATCCATGAGTCGCCGCCTGTGAAAAACTTTCGTGTATCGTGACAATTTTTCGTCAGAGTAAATAACAAAATTCgg < 2:52584/143‑1 (MQ=255) aTGCGCGATGAGATGCGCCAGTGTGACCTGTTTGCCGGGCACAAATTCCTGAATGATGCGTTCTTTATCCATGAGTCGCCGCCTGTGAAAAACTTTCGTGTATCGTGACAATTTTTCGTCAGAGTAAATAACAAAATTCGGCa < 1:244914/143‑1 (MQ=255) tGCGCGATGAGATGCGCCAGTGTGACCTGTTTGCCGGGCACAAATTTCTGAATGATTCGTTCTTTATCCATGAGTCGCCGCCTGTGAAAAACTTTCGTGTATCGTGACAATTTTTCGTCAGAGTAAATAACAAAATTCGGCaa < 1:212799/143‑1 (MQ=255) tGCGCGATGAGATGCGCCAGTGTGACCTGTTTGCCGGGCACAAATTCCTGAATGATGCGTTCTTTATCCATGAGTCGCCGCCTGTGAAAAACTTTCGTGTATCGTGACAATTTTTCGTCAGAGTAAATAACAAAATTCGGCaa < 1:181625/143‑1 (MQ=255) gATGAGATGCGCCAGTGTGACCTGTTTGCCGGGCACAAATTCCTGAATGATGCGTTCTTTATCCATGAGTCGCCGCCTGTGAAAAACTTTCGTGTATCGTGACAATGTTTGGTCAGAGTAAATAACAAAATTCGGCAAGcgcg > 2:240349/1‑143 (MQ=255) aTGCGCCAGTGTGACCTGTTTGCCGGGCACAAATTCCTGAATGATGCGTTCTTTATCCATGAGTCGCCGCCTGTGAAAAACTTTCGTGTATCGTGACAATTTTTCGTCCGCGTAAATAACAAAATTCGGCAAGCGCGTTTaaa > 2:83874/1‑143 (MQ=255) gcgcCAGTGTGACCTGTTTGCCAGGCACAAATTCCTGAATGATGCGTTCTTTATCCATGAGTCGCCGCCTGTGAAAAACTTTCGTGTATCGTGACAATTTTTCGTCAGAGTAAATAACAAAATTCGGCAAGCGCGTTTAAAAg < 1:306961/143‑1 (MQ=255) gcgcCAGTGTGACCTGTTTGCCAGGCACAAATTCCTGAATGATGCGTTCTTTATCCATGAGTCGCCGCCTGTGAAAAACTTTCGTGTATCGTGACAATTTTTCGTCAGAGTAAATAACAAAATTCGGCAAGCGCGTTTAAAAg < 1:308083/143‑1 (MQ=255) gcgcCAGTGTGACCTGTTTGCCAGGCACAAATTCCTGAATGATGCGTTCTTTATCCATGAGTCGCCGCCTGTGAAAAACTTTCGTGTATCGTGACAATTTTTCGTCAGAGTAAATAACAAAATTCGGCAAGCGCGTTTAAAAg < 1:773362/143‑1 (MQ=255) ggcAGTGTGACCTGTTTGCCGGGCACAAATTCCTGAATGATGCGTTCTTTATCCATGAGTCGCCGCCTGTGAAAAACTTTCGTGTATCGTGACAATTTTTCGTCAGAGTAAATAACAAAATTCGGCAAGCGCGTTTAAAAGGt < 2:526722/141‑1 (MQ=255) gcCAGTGTGACCTGTTTGCCGGGCACAAATTCCTGAATGATGCGTTCTTTATCCATGAGTCGCCGCCTGTGAAAAACTTTCGTGTATCGTGACAATTTTTCGTCAGAGTAAATAACAAAATTCGGCAAGCGCGTTTAAAAGGt < 1:259571/143‑1 (MQ=255) ccAGTGTGACCTGTTTGCCGGGCACAAATTCCTGAATGATGCGTTCTTTATCCATGAGTCGCCGCCTGTGAAAAACTTTCGTGTATCGTGACAATTTTTCGTCAGAGTAAATAACAAAATTCGGCAAGCGCGTTTAAAAGGTg < 1:119354/143‑1 (MQ=255) accagaaCCTGTTTGCCGGGCACAAATTCCTGAATGATGCGTTCTTTATCCATGAGTCGCCGCCTGTGAAAAACTTTCGTGTATCGTGACAATTTTTCGTCAGAGTAAATAACAAAATTCGGCAAGCGCGTTTAAAAGGTGAg < 1:547697/137‑1 (MQ=255) tgtgACCTGTTTGCCGGGCACAAATTCCTGAATGATGCGTTCTTTATCCATGAGTCGCCGCCTGTGAAAAACTTTCGTGTATCGTGACAATTTTTCGTCAGAGTAAATAACAAAATTCGGCAAGCGCGTTTAAAAGGTGAGGt < 1:190104/143‑1 (MQ=255) cTGTTTGCCGGGCACAAATTCCTGAATGATGCGTTATTTATCCATGAGTCGCCGCCTGTGAAAAACTTTCGTGTATCGTGACAATTTTTCGTCAGAGTAAATAACAAAATTCGGCAAGCGCGTTTAAAAGGTGAGGTAGATCa > 1:170124/1‑143 (MQ=255) cGGGCACAAATTCCTGAATGATGCGTTCTTTATCCATGAGTCGCCGCCTGTGAAAAACTTTCGTGTATCGTGACAATTTTTCGTCAGAGTAAATAACAAAATTCGGCAAGCGCGTTTAAAAGGTGAGGTAGATCACTACGAAt > 1:171238/1‑143 (MQ=255) gggCACAAATTCCTGAATGATGCGTTCTTTATCCATGAGTCGCCGCCTGTGAAAAACTTTCGTGTATCGTGACAATTTTTCGTCAGAGTAAATAACAAAATTCGGCAAGCGCGTTTAAAAGGTGAGGTAGATCACTAAGAATg < 1:105352/143‑1 (MQ=255) | CGCTAAATCACCGGCGATCATCGCCGTTTCGCCGGGAGTTAGCGTCATAATGCCAATTGCGCCCGCATCCGGAACGCCGATTTTCTTCGCCAGTTCCTCACCGGGATGCGCGATGAGATGCGCCAGTGTGACCTGTTTGCCGGGCACAAATTCCTGAATGATGCGTTCTTTATCCATGAGTCGCCGCCTGTGAAAAACTTTCGTGTATCGTGACAATTTTTCGTCAGAGTAAATAACAAAATTCGGCAAGCGCGTTTAAAAGGTGAGGTAGATCACTAAGAATG > NC_000913/2575629‑2575912 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |