Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,661,969 C→G 100% V55L (GTT→CTT)  iscR ← DNA‑binding transcriptional dual regulator IscR

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,661,9690CG100.0% 155.5 / ‑6.7 52V55L (GTT→CTT) iscRDNA‑binding transcriptional dual regulator IscR
Reads supporting (aligned to +/- strand):  ref base C (0/0);  major base G (28/23);  minor base T (0/1);  total (28/24)
Rejected as polymorphism: E-value score below prediction cutoff.
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

CCGCCCTGGCAGCCGCCTTTACCCTGACAACGGGTGGCATCTACAGATTCGTCAACGGCGCTAATTACTTCGCCAACGGCGATGCTGCTGGCATCTTTGCCTAACAGATAACCACCGCCTGGTCCACGTACGCTGGAAACCAGACCATTTTTACGCAGACGGGAAAACAGTTGTTCCAGATAAGAAAGGGAAATTCCCTGACGTTCGGAAATATCAGCCAACGGTACCGGGCCCGCTTCAGAGTTGAGCGCAACGTCAAGCATTGCGGTCACGGCATA  >  NC_000913/2661830‑2662107
                                                                                                                                           |                                                                                                                                          
ccgccCTGGCAGCCGCCTTTACCCTGACAACGGGTGGCATCTACAGATTCGTCAACGGCGCTAATTACTTCGCCAACGGCAATGCTGCTGGCATCTTTGCCTAACAGATAACCACCGCCTGGTCCACGTACGCTGGAAAGCAg                                                                                                                                         >  1:451364/1‑143 (MQ=255)
  gctcTGGCAGCCGCCTTTACCCTGACAACGGGTGGCATCTACAGATTCGTCAACGGCGCTAATTACTGCGCCAACGGCGATGCTGCTGGCATCTTTGCCTAACAGATAACCACCGCCTGGTCCACGTACGCTGGAAAGCAGAc                                                                                                                                       <  1:262903/140‑1 (MQ=255)
    ccTGGCAGCCGCCTTTACCCTGACAACGGGTGGCATCTACAGATTCGTCAACGGCGCTAATTACTTCGCCAACGGCGATGCTGCTGGCCTCTTTGCCTCACAGATATCCACCGCCTGGTCCACTTTAGCTGGAAAGCAGAccc                                                                                                                                     >  1:216066/1‑142 (MQ=255)
            ccgccTTTGCCCTGACAACGGGTGGCAGCTACAGATTCGTCAACGGCGCTAATTACGTCGCCAACGGCGATGCTGCTGGCATCTTTGCCTAACAGATAACCACCGCCTGGTCCACGTACGCTGGAAAGCAGACCATTTTTACg                                                                                                                             <  2:381294/143‑1 (MQ=255)
             cgccTTTACCCTGACAACGGGTGGCATCTACAGATTCGTCAACGGCGCTAATTACTTCGCCAACGGCGATGCTGCTGGCATCTTTGTCTAACAGATAACCACCGCCTGGTCCACGTACGCTGGAAAGCAGACCATTTTTACGc                                                                                                                            >  1:58658/1‑143 (MQ=255)
                 cttACCCTGCCACCGGGTGGCACCTACAGATTCGTCAACGGCGCTAATTACTTCGCCAACGGCGATGCTGCTGGCATCTTTGCCTAACAGATAACCACCGCCTGGTCCACGTACGCTGGAAAGCAGACCATTTTTACGCAGAc                                                                                                                        <  1:545012/142‑1 (MQ=255)
                      ccTGACAACGGGTGGCATCTACAGATTCGTCAACGGCGCTAATTACTTCGCCAACGGCGATGCTGCTGGCATCTTTGCCTAACAGATAACCACCGCCTGGTCCACGTACGCTGGACAGCAGACCAACTTTACGCCGACGGGaa                                                                                                                   >  2:90838/1‑143 (MQ=255)
                      ccTGACAACGGGTGGCATCTACAGATTCGTCAACGGCGCTAATTACTTCGCCAACGGCGATGCTGCTGGCATCTTTGCCTAACAGATAACCACCGCCTGGTCCACGTACGCTGGAAAGCAGACCATTTTTACGCAGACGGGaa                                                                                                                   <  1:307696/143‑1 (MQ=255)
                          aCAACGGGTGGCATCTACAGATTCGTCAACGGCGCTAATTACTTCGCCAACGGCGATGCTGCTGGCATCTTTGCCTAACAGATAACCACCGCCTGGTCCACGTACGCTGGAAAGCAGACCATTTTTACGCAGACGGGAAAACa                                                                                                               <  1:321994/143‑1 (MQ=255)
                            aaCGGGTGGCATCTACAGATTCGTCAACGGCGCTAATTACTTCGCCAACGGCGATGCTGCTGGCATCTTTGCCTAACAGATAACCACCGCCTGGTCCACGTACGCTGGAAAGCAGACCATTTTTACGCAGACGGGAAAACAgt                                                                                                             >  2:805074/1‑143 (MQ=255)
                                 gTGGCATCTACAGATTCGTCAACTGCGCTAATTACTTCGCCAACGGCGTTGCTGCTGGCATCTTTGCCTAACAGATAACCACCGCGTGGGCCACGTACGCCGGAAAGCAGACCATTTTTACGCAGACGGGAAAACAGTTGTTc                                                                                                        <  1:488901/143‑1 (MQ=255)
                                  tgggttCTACAGATTCGGCAACGGCGCTAATTACTTCGCCAACGGCGATGCTGCTGGCATCTTTGCCTAACAGATAACCACCGCCTGGTCCACGTACGCTGGAAAGCAGACCATTTTTACGCAGACGGGAAAACAGTTGTTcc                                                                                                       <  2:405022/138‑1 (MQ=255)
                                  tGGCATCTACAGATTCGTCAACGGCGCTAATTACTTCGCCAACGGCGATGCTGCTGGCATCTTTGCCTAACAGATAACCACCGCCTGGTCCACGTACGCTGGAAAGCAGACCATTTTTACGCAGACGGGAAAACAGTTGTTcc                                                                                                       >  2:461262/1‑143 (MQ=255)
                                  ggccATCTACAGACTCGTCGACGGCGCTAACTACTTCGCCAACGGCGCTGCTGCTGGCATCTTTGCATAACAGAGAACCACCGCCTGGTCCACGTACGCTGGAAAGCAGACCATTTTTACGCAGACGGGAAAACAGTTGTTcc                                                                                                       <  2:603404/140‑1 (MQ=255)
                                    gCATCTACAGATTCGTCAACGGCGCTAATTACTTCGCCAACGGCGATGCTGCTGGCATCTTTGCCTAACAGATAACCACCGCCTGGTCCACGTACGCTGGAAAGCAGACCATTTTTACGCAGACGGGAAAACAGTTGTTCCAg                                                                                                     <  1:442930/143‑1 (MQ=255)
                                     cATCTACAGATTCGTCAACGGCGCTAATTACTTCGCCAACGGCGATGCTGCTGGCATCTTTGCCTAACAGATAACCACCGCCTGGTCCACGTACGCTGGAAAGCAGACCATTTTTACGCAGACGGGAAAACAGTTGTTCCAGa                                                                                                    <  1:89355/143‑1 (MQ=255)
                                      aTCTACAGATTCGTCAACGGCGCTAATTACTTCGCCAACGGCGATGCTGCTGGCATCTTTGCCTAACAGATAACCACCGCCTGGTCCACGTACGCTGGAACGCAGACCATTTTTACGCAGACGGGAAAACAGTTGTTCCAGAt                                                                                                   >  2:704229/1‑143 (MQ=255)
                                         tACAGATTCGTCAACGGCGCTAATTACTTCGCCAACGGCGATGCTGCTGGCATCTTTGCCTAACAGATAACCACCGCCTGGTCCACGTACGCTGGAAAGCAGACCATTTTTACGCAGACGGGAAAACAGTTGTTCCAGATAAg                                                                                                >  1:45687/1‑143 (MQ=255)
                                          aCAGATTCGTCAACGGCGCTAATTACTTCGCCAACGGCGATGCTGCTGGCATCTTTGCCTAACAGATAACCACCGCCTGGTCCACGTACGCTGGAAAGCAGACCATTTTTACGCAGACGGGAAAACAGTTGTTCCAGATAAGa                                                                                               >  2:597735/1‑143 (MQ=255)
                                            ggATTCGTCAACGGCGCTAAGCACTTCGCCAACGGCGATGCTGCTGGCAGCTTTGCCTAACAGATAACCACCGCCTGGTCCACGTACGCTGGAAAGCAGACCATTTTTACGCAGACGGGAAAACAGTTGTTCCAGATAAGaaa                                                                                             <  2:476664/142‑1 (MQ=255)
                                             gATTCGTCAACGGCGCTAATTACTTCGCCAACGGCGATGCTGCTGGCATCTTTGCCTAACAGATAACCACCGCCTGGTCCACGTACGCTGGAAAGCAGACCATTTTTACGCAGACGGGAAAACAGTTGTTCCAGATAAGAAAg                                                                                            <  1:597735/143‑1 (MQ=255)
                                             gATTCGTCAACGGCGCTAATTACTTCGCCAACGGCGATGCTGCTGGCATCTTTGCCTAACAGATAACCACCGCCTGGTCCACGTACGCTGGAAAGCAGACCATGTTTACGCAGACGGGAAAACAGTCGTTCCAGATAAGAAAg                                                                                            >  2:521481/1‑143 (MQ=255)
                                              aTTCGTCAACGGCGCTAATTACTTCGCCAACGGCGATGCTGCTGGCATCTTTGCCTAACAGATAACCACCGCCTGGTCCACGTACGCTGGAAAGCAGACCATTTCTACGCAGCCGGGGAACCACTTGGTGCAGATACGACAgg                                                                                           >  2:651361/1‑143 (MQ=255)
                                                   tcgaCGGCGCTACTTACTTCGCCAAGGGCGATGCTGCTGGCTTCTTTGCCTAACAGATAACCGCTGCCTGGTCCACGTACGCTGGAAAGCAGACCATTTTTACGCAGTCGGGAAAACAGTTGTTCCAGATAAGAAAGGGAAAt                                                                                      <  2:721533/140‑1 (MQ=255)
                                                   tCAACGGCGCTAATTACTTCGCCAACGGCGATGCTGCTGGCATCTTTGCCTAACAGATAACCACCGCCTGGTCCACGTACGCTGGAAAGCAGACCATTTTTACGCAGACGGGAAAACCGTTGATCCAGACAAGCAAGGCaaac                                                                                      >  2:459711/1‑142 (MQ=255)
                                                     aaCGGCGCTAATTACTTCGCCAACGGCGATGCTGCTGGCATCTTTGCCTAACAGATAACCACCGCCTGGTCCACGTACGCTGGAAAGCAGACCATTTTTACGCAGACGGGAAAACAGTTGTTCCAGATAAGAAAGGGAAATTc                                                                                    >  1:674827/1‑143 (MQ=255)
                                                      aCGGCGCTAATTACTTCGCCAACGGCGATGCTGCTGGCATCTTTGCCTAACAGATAACCACCGCCTGGTCCACGTACGCTGGAAAGCAGACCATTTTTACGCAGACGGGAAAACAGTTGTTCCAGATAAGAAAGGGAAATTcc                                                                                   <  1:689791/143‑1 (MQ=255)
                                                        ggCGCGTATTACTTCGCCAACGGCGAGGCTGCTGGCATCTTTGCCTAACAGATAACCACCGCCTGGTCCACGTACGCTGGAAAGCAGACCATTTTTACGCAGACGGGAAAACAGTTGTTCCAGATAAGAAAGGGAAATTCCCt                                                                                 <  2:276256/143‑1 (MQ=255)
                                                        ggCGCGAAGGACTTCGCCAACGGCGATGCTGCTGGCATCTTTGCCTAACAGATAACCACCGCCTGGTCCACGTACGCTGGAAAGCAGACCATTTTTACGCAGACGGGAAAACAGTTGTTCCAGATAAGAAAGGGAAATTCCCt                                                                                 <  2:451364/143‑1 (MQ=255)
                                                               aTTACTTCGCCAACGGCGATGCTGCTGGCATCTTTGCCTAACAGATAACCACCGCCTGGTCCACGTACGCTGGAAAGCAGACCATTTTTACGCAGACGGGAAAACAGTTGTTCCAGATAAGAAAGGGAAATTCCCTGACGTTc                                                                          <  1:805074/143‑1 (MQ=255)
                                                                     tCGCCAACGGCGGTGCTGCTGGCATCTTTGCCTAACAGATAACCACCGCCTGGTCCACGTACGCTGGAAAGCAGACCATTTTTACGCAGACGGGAAAACAGTTGTTCCAGATAAGAAAGGGAAATTCCCTGACGTTCGGAAat                                                                    <  2:640303/143‑1 (MQ=255)
                                                                      cGCCAACGGCGATGCTGCTGGCATCTTTGCCTAACAGATAACCACCGCCTGGTCCACGTACGCTGGAAAGCAGACCATTTTTACGCAGACGGGCAAACAGTTGTTCCAGATAAGAAGGGGAAATTCCCTGCCGCTCGGAAata                                                                   >  1:286275/1‑143 (MQ=255)
                                                                        ccAACGGCGATGCTGCTGGCATCTTTGCCTAACAGATAACCACCGCCTGGTACACGTACGCTGGAAAGCAGAACATATTTACGCAGACGGGAAAACAGTTGTTCCAGATAGGAGAGGGAAATGCGCCGACGATCGGgaacagc                                                                 >  2:718318/1‑139 (MQ=255)
                                                                           aCGGCGATGCTGCTGGCATCTTTGCCTAACAGATAACCACCGCCTGGTCCACGTACGCTGGAAAGCAGACCATTTTTACGCAGACGGGAAAACAGTTGTTCCAGATAAGAAAGGGAAATTCCCTGACGTTCGGAAATATCAGc                                                              <  2:287069/143‑1 (MQ=255)
                                                                            cGGCGATGCTGCTGGCATCTTTGCCTAACAGATAACCACCGCCTGGTCCACGTACGCTGGAAAGCAGACCATTTTTACGCAGACGGGAACACAGTTGTTCCAGATAAGAAAGGGAAATGCCCTGACGTTCGGAACTATCAGcc                                                             >  2:805539/1‑143 (MQ=255)
                                                                                gATGCTGCTGGCATCTTTGCCTAACAGATAACCACCGCCTGGTCCACGTACGCTGGAACGCAGACCATTTTTACGCAGACGGGAAAACAGTTGTTCCAGATAAGAAAGGGAAATTCCCTGACGTTCGGAAATATCAGCCAACg                                                         >  2:852456/1‑143 (MQ=255)
                                                                                     tgcttGCCTCTTTGCCTAGCTGGTAATCTCCGTCTGGCCCTCTTATGCTGCAAAGCAGATCATTTTTACGCAGACGGGAAGACAGTTGTTCCCGATAAGAAAGGGAAATTCCCTGACGTTCGGAAATATCATCCAACGGTAcc                                                    <  2:609316/143‑1 (MQ=255)
                                                                                         ggCACCTTGGCCTAACAGAGAACCACCGCCTGGTCCACGTACGCTGGAAAGCACACCATTTTTACGCAGACGGGAAAACAGTTGTTCCAGATAATAAAGGGAAATTCCCTGACGTTCGGAAATATCAGCCAACGGTACCGGGc                                                <  1:332354/143‑1 (MQ=255)
                                                                                          gCATCTTGCCCTAACAGCTAACCGCCGGCTGGTCCACGTACGCTGGAAAGCAGACCATTTTTACGCAGTCGGGAACACCGTTGTTCCAGATAAGACAGGGAAATTCCCTGACGTTCGGAAATATCAGCCAACAGTACCGGGcc                                               <  2:499670/143‑1 (MQ=255)
                                                                                                ttGCCTAACAGATAACCACCGCCTGGTCCACGTACGCTGGAAAGCAGACCATTTTTACGCCGACGGGAAAACCGTTGTTCCAGATAAGAAAGGGAAATTCCCTGCCGTTCGGAAATATCAGCCAACGGCACCCGGCCCGCTTc                                         >  2:170677/1‑143 (MQ=255)
                                                                                                      aaCAGATAACCACCGCCGGGTCCACGGACGCTGGAAAGCAGACCATTTTTACGCAGACGGGAAAACAGTTGTTCCAGATAAGAAAGGGAAATTCCCTGACGTTCGGAAATATCAGCCAACGGTACCGGGCCCGCTTCAGAGtt                                   <  1:672104/143‑1 (MQ=255)
                                                                                                         aGATAACCACCGCCTGGTCCACGTACGCTGGAAAGCAGACCATTTTTACGCAGACGGGAAAACAGTTGTTCCAGATAAGAAAGGGAAATTCCCTGACGTTCGGAAATATCAGCCAACGGTACGGGGCCCGCTTCAGAGTTGAg                                >  1:251257/1‑143 (MQ=255)
                                                                                                         aGATAACCACCGCCTGGTCCACGTACGCTGGAAAGCAGACCATTTTTACGCAGACGGGAAAACAGTTGTTCCAGATAAGAAAGGGAAATTCCCTGACGTTCGGAAATATCAGCCAACGGTACCAGGCCCGCTTCCGAGTTGAg                                >  1:445689/1‑143 (MQ=255)
                                                                                                          gATAACCACCGCCTGGTCCACGTACGCTGGAAAGCAGACCATTTTTACGCAGACGGGAAAACAGTTGTTCCAGATAAGAAAGGGAAATTCCCTGACGTTCGGAAATATCAGCCAACGGTACCGGGCCCGCTTCAGAGTTGAgc                               >  1:626321/1‑143 (MQ=255)
                                                                                                           aTAACCACCGCCTGGTCCACGTACGCTGGAAAGCAGACCATTTTTACGCAGACGGGAAAACAGTTGTTCCAGATAAGAAAGGGAAATTCCCTGACGTTCGGAAATATCAGCCAACGGTACCGGGCCCGCCTCAGAGTTGAgcg                              >  2:199449/1‑143 (MQ=255)
                                                                                                             aaCCACCGCCTGGTCCACGTACGCTGGAAAGCAGACCATTTTTACGCAGACGGGAAAAAAGTTGTTCCAGATAAGAAAGGGAAATTCCCTGACGTTCGGAAATATCAGCCACCGGTACCGGGCCCGCTTCAGAGTTGGGCGCa                            >  1:398429/1‑143 (MQ=255)
                                                                                                               cctccgccgTGTCCGCGTACGCTGTAAATCAGCCCCTTTTCACGCAGACGGTAAATCAGTTGTTCCAGATAAGAAAGGGTAATTCCCTGACGTTCGGAAATATCAGCCAACGGTACCGGGCCCGCTTCATAGTTGAGCGCAAc                          <  2:45687/140‑1 (MQ=255)
                                                                                                               ccaccGCCTGGTCCACGTACGCTGGAAAGCAGACCATTTTTACGCAGACGGGAAAACAATTGTTCCAGATAAGAAAGGGAAATTCCCTGACGTTCGGAAATATCAGCCAACGGTACCGGGCCCGCTTCAGAGTTGAGCGCAAc                          >  2:444688/1‑143 (MQ=255)
                                                                                                                  ccGCCTGGTCCACGTACGCTGGAAAGCAGACCATTTTTACGCAGACGGGAAAACAGTTGTTCCAGATAAGAAAGGGAAATTCCCTGACGTTCGGAAATATCAGCCAACGGTACCGGGCCCGCTTCAGAGTTGAGCGCAACGTc                       >  1:677181/1‑143 (MQ=255)
                                                                                                                       tGGTCCACGTACGCTGGAAAGCAGACCATTTTTACGCAGACGGGAAAACAGTTGTTCCAGATAAGAAAGGGAAATTCCCTGACGTTCGGAAATATCAGCCAACGGTACCGGGCCCGCTTCAGAGTTGAGCGCAACGTCAAGCa                  >  1:13351/1‑143 (MQ=255)
                                                                                                                                    cTGGAAAGCAGACCATTTTTACGCAGACGGGAAAACAGTTGTTCCAGATAAGAAAGGGAAATTCCCTGACGTTCGGAAATATCAGCCAACGGTACCGGGCCCGCTTCAGAGTTGAGCGCAACGTCAAGCATTGCGGTCACGGc     >  2:703371/1‑143 (MQ=255)
                                                                                                                                       gAAAGCAGACCATTTTTACGCAGACGGGAAAACAGTTGTTCCAGATAAGAAAGGGAAATTCCCTGACGTTCGGAAATATCAGCCAACGGTACCGGGCCCGCTTCAGAGTTGAGCGCAACGTCAAGCATTGCGGTCACGGCATa  >  1:445645/1‑143 (MQ=255)
                                                                                                                                           |                                                                                                                                          
CCGCCCTGGCAGCCGCCTTTACCCTGACAACGGGTGGCATCTACAGATTCGTCAACGGCGCTAATTACTTCGCCAACGGCGATGCTGCTGGCATCTTTGCCTAACAGATAACCACCGCCTGGTCCACGTACGCTGGAAACCAGACCATTTTTACGCAGACGGGAAAACAGTTGTTCCAGATAAGAAAGGGAAATTCCCTGACGTTCGGAAATATCAGCCAACGGTACCGGGCCCGCTTCAGAGTTGAGCGCAACGTCAAGCATTGCGGTCACGGCATA  >  NC_000913/2661830‑2662107

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑