| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_000913 | 3,041,632 | T→A | 100% | V107E (GTG→GAG) | ygfZ → | folate‑binding protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 3,041,632 | 0 | T | A | 99.7% | 157.6 / ‑7.1 | 58 | V107E (GTG→GAG) | ygfZ | folate‑binding protein |
| Reads supporting (aligned to +/- strand): ref base T (0/1); major base A (32/23); minor base C (1/0); total (33/25) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.49e-01 | |||||||||||
| Rejected as polymorphism: E-value score below prediction cutoff. | |||||||||||
| Rejected as polymorphism: Frequency below/above cutoff threshold. | |||||||||||
| Rejected as polymorphism: Variant not supported by required number of reads on each strand. | |||||||||||
GCCCATTGCGACGCCAAAGGTAAAATGTGGAGCAATTTACGTCTGTTCCGCGACG‑GCGATGGCTTTGCATGGATTG‑AACGGCGCAGCGTGCGTGAACCGCAGCTGACTGAACTGAAAAAATATGCGGTATTCTCTAAAGTGACCATCGCGCCAGACGACGAGCGTGTGCTGCTTGGTGTTGCCGGTTTTCAGGCGCGCGCCGCGCTGGCAAATCTCTTTAGCGAACTGCCTTCGAAAGAAAAACAGGTAGTCAAAGAAGGCGCGACCACTTTGCTATGGT > NC_000913/3041493‑3041772 | gccCATTGCGACGCCAAAGGTAAAATGTGGAGCAATTTACGTCTGTTCCGCGACG‑GCGATGGCTTTGCATGGATTG‑AACGGCGCAGCGTGCGTGAACCGCAGCTGACTGAACTGAAAAAATATGCGGTATTCTCTAAAGAGAc > 1:525992/1‑143 (MQ=255)gccCATTGCGACGCCAAAGGTAAAATGTGGAGCAATTTACGTCTGTTCCGCGACG‑GCGATGGCTTTGCATGGATTG‑AACGGCGCAGCGTGCGTGAACCGCAGCTGACTGAACTGAAAAAATATGCGGTATTCTCTAAAGAGAc > 1:220750/1‑143 (MQ=255) ccAAAGGTAAAATGTGGAGCAATTTACGTCTGTTCCGCGACG‑GCGATGGCTTTGCATGGATTG‑AACGGCGCAGCGTGCGTGAACCGCAGCTGCCTGAACTGAAAAAATATGCGGTATTCTATAAAGAGACCATCGCGCCAgac > 1:15201/1‑143 (MQ=255) cAAAGGTAAAATGTGGAGCAATTTACGTCTGTTCCGCGACG‑GCGATGGCTTTGCATGGATTG‑AACGGCGCAGCGTGCGTGAACCGCAGCTGACTGAACTGAAAAAATATGCGGTATTCTCTAAAGAGACCATCGCGCCAgacg > 1:640213/1‑143 (MQ=255) cAAAGGTAAAATGTGGAGCAATTTACGTCTGTGCCGCGACG‑GCGATGGCTTTGCATGGATTG‑AACGGCGCAGCGTGCGTGAACCGCAGCTGACTGAACTGAAAAAATATGCGGTATTCTCTAAAGAGACCATCGCGCCAgacg < 1:437608/143‑1 (MQ=255) cAAAGGTAAAATGTGGAGCAATTTACGTCTGCT‑GGATATGCTCTATGGCTTTGCATGGATTG‑AACGGCGCAGCGTGCGTGAACCGCAGCTGACTGAACTGAAAAAATATGCGGTATTCTCTAAAGAGACCATCGCGCCAgacg < 2:866337/143‑1 (MQ=255) aaaGGTAAAATGTGGAGCAATTTACGTCTGTTCCGCGACG‑GCGACGGC‑TACCACGGCCAGAAACGGCGCAGCGTGCGTGAACCGCAGCTGACTGAACTGAAAAAATATGCGGTATTCTCTAAAGAGACCATCGCGCCAgacga < 1:888002/143‑1 (MQ=255) gTAACATGTGGAGCAATTTCTGTCTGTTCCGCGACG‑GCGATTGCTTTGCATGGATTG‑AACGGCGCAGCGTGCGTGAACCGCAGCTGACTGAACTGAAAAAATATGCGGTATTCTCTAAAGAGACCATCGCGCCAGACGACGAg < 2:46115/143‑1 (MQ=255) aaaTGTGGAGCAATTTACGTCTGTTCCGCGACG‑GCGATGGCTTTGCATGGATTG‑AACGGCGCAGCGTGCGTGAACCGCAGCTGACTGAACTGAAAAAATATGCGGTATTCTCTACAGCGACCATCGCGCCAGACGAGGAGCgt > 1:102605/1‑143 (MQ=255) aaaTGTGGAGCAATTTACGTCTGTTCCGCGACG‑GCGATGGCTTTGCATGGATTG‑AACGGCGCAGCGTGCGTGAACCGCAGCTGACTGAACTGAAAAAATATGCGGTATTCTCTAAAGAGACCATCGCGCCAGACGACGAGCgt > 1:895154/1‑143 (MQ=255) tgGAGCAATTTACGTCTGTTCCGCGACG‑GCGATGGCTTTGCATGGATTG‑AACGGCGCAGCGTGCGTGAACCGCAGCTGACTGAACTGAAAAAATATGCGGTATTCTCTAAAGAGACCATCGCGCCAGACGACGAGCGTGcgct > 2:309731/1‑143 (MQ=255) ttttACTTTTTTTCCGCCACG‑GCTATGGCTTTGCATGGATTG‑AACGGCGCAGCGTGCGTGAACCGCAGCTGACTGAACTGAAAAAATATGCGGTATTCTCTAAAGAGACCATCGCGCCAGACGACGAGCGTGTGCTGCTTGgt < 1:839241/142‑1 (MQ=255) tttACGTCTGTTCCGCGACG‑GCGATGGCTTTGCATGGATTG‑AACGGCGCAGCGTGCGTGAACCGCAGCTGACTGAACTGAAAAAATATGCGGTATTCTCTAAAGAGACCATCGCGCCAGACGACGAGCGTGTGCTGCTTGgtg > 1:7553/1‑143 (MQ=255) tACGTCTGTTCCGCGACG‑GCGATGGCTTTGCATGGATTG‑AACGGCGCAGCGTGCGTGAACCGCAGCTGACTGCACTGCAAAAATATGCGGTATTCTCTAAAGAGACCATCGCGCCAGACGACGAGCGTGGGCTGCTTGGCGtt > 2:524361/1‑143 (MQ=255) aCGTCTGTTCCGCGACG‑GCGATGGCTTTGCATGGATTG‑AACGGCGCAGCGTGCGTGAACCGCAGCTGACTGAACTGAAAAAATATGCGGTATTCTCTAAAGAGACCATCGCGCCAGACGACGAGCGTGTGCTGCTTGGTGTTg > 1:217295/1‑143 (MQ=255) gTCTGTTCCGCGACG‑GCGATGGCTTTGCATGGATTG‑AACGGCGCAGCGTGCGTGAACCGCAGCTGACTGAACTGAAACAATATCCGGCATTCCCTAAAGAGACCATCGCGCCAGACGACGAGCGTGTGCTGCTTGGTGTTGcc > 2:632386/1‑143 (MQ=255) tCTGTTCCGCGACG‑GCGATGGCTTTGCATGGATTG‑AACGGCGCAGCGTGCGTGAACCGCAGCTGACTGAACTGAAAAAATATGCGGTATTCTCTAAAGAGACCATCGCGCCAGACGACGAGCGTGTGCTGCTTGGTGTTGCCg > 1:554518/1‑143 (MQ=255) tCTGTTCCGCGACG‑GCGATGGCTTTGCATGGATTG‑AACGGCGCAGCGTGCGTGAACCGCAGCTGACTGAACTGAAAAAATATGCGGTATTCTCTAAAGAGACCATCGCGCCAGACGACGAGCGTGTGCTGCTTGGTGTTGCCg > 1:554574/1‑143 (MQ=255) tCTGCTCCGCGACG‑GCGATGGCTTTGCATGGATTG‑AACGGCGCAGCGTGCGTGAACCGCAGCTGACTGAACTGAAAAAATATGCGGTATTCTCTAAAGAGACCATCGCGCCAGACGACGAGCGTGTGCTGCTTGGTGTTGCCg < 1:284677/143‑1 (MQ=255) tGTTCCGCGACG‑GCGATGGCTTTGCATGGATTG‑AACGGCGCAGCGTGCGTGAACCGCAGCTGACTGAACTGAAAAAATATGCGGTATTCTCTAAAGAGACCATCGCGCCAGACGATGAGCGTGTGCTGCTTGGTGTTGCCggg > 2:842281/1‑142 (MQ=255) tGTTCCGCGACG‑GCGATGGCTTTGCATGGATTG‑AACGGCGCAGCGTGCGTGAACCGCAGCTGACTGAACTGAAAAAATATGCGGTATTCTCTAAAGAGACCATCGCGCCAGACGACGCGCGTGTGCTGCTTGGTGTGGCCggc > 2:269572/1‑142 (MQ=255) gATGGCTTTGCATGGATTG‑AACGGCGCAGCGTGCGTGAACCGCAGCTGACTGAACTGAAAAAATATGCGGTATTCTCTAAAGAGACCATCGCGCCAGACGACGAGCGTGTGCTGCTTGGTGTTGCCGGTCTTCAGgcgcgcgc > 1:144049/1‑143 (MQ=255) ttGCATGGATTG‑AACGGCGCAGCGTGCGTGAACCGCAGCTGACTGAACTGAAAAAATATGCGGTATTCTCTAAAGAGACCATCGCGCCAGACGACGAGCGTCTGCTGCTTGGTGTTGCCGGTTTTCAGGCGCGCGCCGCGCTg > 2:222641/1‑143 (MQ=255) aTGGATTG‑AACGGCGCAGCGTGCGTGAACCGCAGCTGACTGAACTGAAAAAATATGCGGTATTCTCTAAAGAGACCATCGCGCCAGACGACGAGCGTGTGCTGCTTGGTGTTGCCGGTTTTCAGGCGCGCGCCGCGCTGGCaa > 1:57044/1‑143 (MQ=255) tGGATTG‑AACGGCGCAGCGTGCGTGAACCGCAGCTGACTGAACTGAAAAAATATGCGGTATTCTCTAAAGAGACCATCGCGCCAGACGACGAGCGTGTGCTGCTTGGTGTTGCCGGTTTTCAGGCGCGCGCCGCGCTGGCaaa > 1:208299/1‑143 (MQ=255) ggATTG‑GACGGCGCAGCGTGCGTGAACCGCAGCTGACTGAACTGAAAAAATATGCGGTATTCTCTAAAGAGACCATCGCGCCAGACGACGAGCGTGTGCTGCTTGGTGTTGCCGGTTTTCAGGCGCGCGCCGCGCTGGCAAAt < 2:709316/143‑1 (MQ=255) gATTG‑AACGGCGCAGCGTGCGTGAACCGCAGCTGACTGAACTGAAAAAATATGCGGTATTCTCTAAAGAGACCATCGCGCCAGACGACGAGCGTGTGCTGCTTGGTGTTGCCGGTTTTCAGGCGCGCGCCGCGCTGACAAAtc < 1:60017/143‑1 (MQ=255) ttG‑AACGGCGCAGCGTGCGTGAACCGCAGCTGACTGAACTGAAAAAATATGCGGTATTCTCTAAAGAGACCATCGCGCCAGACGACGAGCGTGTGCTGCCTGGTGTTGCCGGTCTTCCGGCGCCCGCCGCCCTGCCaaagccc > 2:648207/1‑139 (MQ=255) tG‑AACGGCGCAGCGTGCGTGAACCGCAGCTGACTGAACTGAAAAAATATGCGGTATTCTCTAAAGAGACCATCGCGCCAGACGACGAGCGTGTGCTGCTTGGTGTTGCCGGGTTTCAGGCGCGCGTCGCGCTGGCAActctct > 2:684941/1‑143 (MQ=255) aaCGGCGCAGCGTGCGTGAACCGCAGCTGACTGAACTGAAAAAATATGCGGTATTCTCTAAAGAGACCATCGCGCCAGACGACGAGCGTGTGCTGCTTGGTGTTGCCGGTTTTCAGGCGCGCGCCGCGCTGGCAAATCTCttt < 1:369738/143‑1 (MQ=255) aCGGCGCAGCGTGCGTGAACCGCAGCTGACTGAACTGAAAAAATATGCGGTATTCTCTAAAGAGACCATCGCGCCAGACGACGAGCGTGTGCTGCTTGGTGTTGCCGGTTTTCAGGCGCGCGCCGCGCTGGCAAATCTCTTTa < 1:427085/143‑1 (MQ=255) aCGGCGCAGCGTGCGTGAACCGCAGCTGACTGAACTGAAAAAATATGCGGTATTCTCTAAAGAGACCATCGCGCCAGACGACGAGCGTGTGCTGCTTGGTGTTGCCGGTTTTCAGGCGCGCGCCGCGCTGGCAAATCTCTGTa > 2:611847/1‑143 (MQ=255) cGGCGCAGCGTGCGTGAACCGCAGCTGACTGAACTGAAAAAATATGCGGTATTCTCTAAAGAGACCATCGCGCCAGACGACGAGCGTGTGCTGCTTGGTGTTGCCGGTTTTCAGGCGCGCGCCGCGCTGGCAAATCTCTTTAg < 1:674491/143‑1 (MQ=255) ggCGCAGCGTGCGTGAACCGCAGCTGACTGAACTGAAAAAATATGCGGTATTCACTAAAGAGACCATCGCAGCCGACGACGGGCGTGAGCGGCCTGGTGTTGCCGGATATCAGGCGCGCGCCGCGCCGGCAAAACACATTAGc > 2:775815/1‑143 (MQ=255) cgcAGCGTGCGTGAACCGCAGCTGACTGAACTGAAAAAATATGCGGTATTCTCTAAAGAGACCATCGCGCCAGACGACGAGCGTGTGCTGCTTGGTGTTGCCGGTTTTCAGGCGCGCGCCGCGCTGGCAAATCTCTTTAGCGa > 1:350310/1‑143 (MQ=255) gcAGCGTGCGTGAACCGCAGCTGACTGAACTGAAAAAATATGCGGTATTCTCTAAAGAGACCATCGCGCCAGACGACGAGCGTGTGCTGCTTGGTGTTGCCGGTTTTCAGGCGCGCGCCGCGCTGGCAAATCTCTTTAGCGaa < 1:366041/143‑1 (MQ=255) cAGCGTGCGTGAACCGCAGCGGACTGAACTGAAAAAATATGCGGTATTCTCTAAAGAGACCATCGCGCCAGACGGCGAGCGTGTGCTGCTTGGTGTTGCCGGTTTTCAGGCGCGCGCCGCGCTGGCAAATCTCTTTAGCGAAc < 2:551580/143‑1 (MQ=255) cgtgACCCTCGGCGGACTGAACTGAAATAATATTCGGTATTCTCTAACGAGACCATCGCGCTAGACGACGAGCGTGTGCTGCTTGGGGTTGCCGGTTTTCAGGCGCGCGCCGCGCTGGCAAATCTCTTTAGCGAACTGCCTTc < 2:659117/143‑1 (MQ=255) gtgAACCGCAGCTGACTGAACTGAAAAAATATGCGGTATTCTCTAAAGAGACCATCGCGCCAGACGACGAGCGTGTGCTGCTTGGTGTTGCCGGTTTTCAGGCGCGCGCCGCGCTGGGCGACCGCTTTAGCGAACTGCCTTCg > 2:140479/1‑143 (MQ=255) tgAACCGCAGCTGACTGAACTGAAAAAATATGCGGTATTCTCTAAAGAGACCATCGCGCCAGACGACGAGCGTGTGCTGCTTGGTGTTGCCGGTTTTCAGGCGCGCGCCGCGCTGGCAAATCTCTTTAGCGAACTGCCTTCga > 2:891850/1‑143 (MQ=255) gAACCGCAGCTGACTGAACTGAAAAAATATGCGGTATTCTCTAAAGAGACCATCGCGCCAGACGACGAGCGTGTGCTGCTTGGTGTTGCCGGTTTTCAGGCGCGCGCCGCGCTGGCAAATCTCTTTAGCGAACTGCCTTCgaa < 1:320941/143‑1 (MQ=255) aaCCGCAGCCGACGGAACTGAAAAAATATGCGGTATTCTCTAAAGAGACCATCGCGCCAGACGACGAGCGTGTGCTGCTTGGTGTTGCCGGTTTTCAGGCGCGCGCCGCGCTGGCAAATCTCTTTAGCGAACTGCCTTCgaaa < 1:857693/143‑1 (MQ=255) aCCGCAGCTGACTGAACTGAAAAAATATGCGGTATTCTCTAAAGAGACCATCGCGCCAGACGACGAGCGTGTGCTGCTTGGTGTTGCCGGTTTTCAGGCGCGCGCCGCGCTGGCAAATCTCTTTAGCGAACTGCCTTCgaaag < 1:460926/143‑1 (MQ=255) cGCAGCTGACTGAACTGAAAAAATATGCGGTATTCTCTAAAGAGACCATCGCGCCAGACGACGAGCGTGTGCTGCTTGGTGTTGCCGGTTTTCAGGCGCGCGCCGCGCTGGCAAATCTCTTTATCGAACTGCCTTCgaaagaa > 1:38673/1‑143 (MQ=255) cGCAGCTGACTGAACTGAAAAAATATGCGGTATTCTCTAAAGAGACCATCGCGCCAGACGACGAGCGTGTGCTGCTTGGTGTTGCCGGTTTTCAGGCGCGCGCCGCGCTGGCAAATCTCTTTAGCGAACTGCCTTCgaaagaa > 1:694311/1‑143 (MQ=255) gCAGCTGGGTGAACTGAAAAAATATGCGGTATTCTCTAAAGAGACCATCGCGCCAGACGACGAGCGTGTGCTGCTTGGTGTTGCCGGTTTTCAGGCGCGCGCCGCGCTGGCAAATCTCTTTAGCGAACTGCCTTCgaaagaaa < 2:648539/143‑1 (MQ=255) tgactgaACTGAAAAAATATGCGGTATTCACTAAAGAGACCATCGCGCCAGACGACGAGCGTGTGCTGCTTGGTGTTGCCGGTTTTCAGGCGCGCGCCGCGCTGGCAAATCTCTCTATCGCACTGGCTTCGCACGAACAACAg > 2:163131/1‑143 (MQ=255) tgaaAAAATATGCGGTATTCTCTAAAGAGACCATCGCACCAGACGACGCGCGTGTGCTGCTTGGTGTTGCCGGAGTTCAGGCGCGCGCCGCGCTGGCAAATCTCTTTAGCGAACCGCCCTCGAAAGACAAACAGGTAGtgaga > 2:107966/1‑139 (MQ=255) aaaaaaTATGCGGTATTCTCTAAAGAGACCATCGCGCCAGACGACGAGCGTGTGCTGCTTGGTGTTGCCGGTTTTCAGGCGCGCGCCGCGCTGGCAAATCTCTTTAGCGAACTGCCTTCGAAAGAAAAACAGGTAGTCAaaga > 2:711414/1‑143 (MQ=255) aaaaaTATGCGGTATTCTCTAAAGAGACCATCGCGCCAGACGACGAGCGTGTGCTGCTTGGTGTTGCCGGTTTTCAGGCGCGCGCCGCGCTGGCAAATCTCTTTAGCGAACTGCCTTCGAAAGAAAAACAGGTAGTCAaagaa < 2:895154/143‑1 (MQ=255) aaaaTATGCGGTATTCTCTAAAGAGACCATCGCGCCAGACGACGAGCGTGTGCTGCTTGGTGTTGCCGGTTTTCAGGCGCGCGCCGCGCTGGCAAATCTCTTTAGCGAACTGCCTTCGAAAGAAAAACAGGTAGTCAaagaag < 1:520626/143‑1 (MQ=255) tGCGGTAGTCTCTAAAGAGACCATCGCGCCAGACGACGAGCGTGTGCTGCTTGGTGTTGCCGGTTTTCAGGCGCGCGCCGCGCTGGCAAATCTCTTTAGCGAACTGCCTTCGAAAGAAAAACAGGTAGTCAAAGAAGGCGCGa < 2:679835/143‑1 (MQ=255) tctcTAAAGAGTTCATCGCGCCAGACTCCCAGCTTGTGCTGCTTGGTGTTGCCTGTTTTCAGGCGCGCGCCGCGCTGGCAAATCTCTTTAGCGAACTGCCTTCGAAAGAAAAACAGGTAGTCAAAGAAGGCGCGACCACTTTg < 1:876297/143‑1 (MQ=255) tctAAAGAGACCATCGCGCCGGGCGACGAGCGTGTGCTGCTTGGTGTTGCCGGTTTTCAGGCGCGCGCCGCGCTGGCAAATCTCTTTAGCGAACTGCCTTCGAAAGAAAAACAGGTAGTCAAAGAAGGCGCGACCACTTTGCt < 1:211707/143‑1 (MQ=255) tctAAAGAGACCATCGCGCCAGACGACGAGCGTGTGCTGCTTGGTGTTGCCGGTTTTCAGGCGCGCGCCGCGCTGGCAAATCTCTTTAGCGAACTGCCTTCGAAAGAAAAACAGGTAGTCAAAGAAGGCGCGACCACTTTGCt > 1:819702/1‑143 (MQ=255) tctAAAGAGACCATCGCGCCAGACGACGAGCGTGTGCTGATTGGTGTTGCCGGTTTTCAGGCGCGCGCCGCGCTGGCAAATCTCTTTAGCGAACTGCCTTCGAAAGAAAAACAAGTAGTCAAAAAAAGCGCAACCAGTTTACt > 2:23342/1‑143 (MQ=255) aaaGGGACCATCGCGCCAGACGACGAGCGTGTGCTGCTTGGTGTTGCCGGTTTTCAGGCGCGCGCCGCGCTGGCAAATCTCTTTAGCGAACTGCCTTCGAAAGAAAAACAGGTAGTCAAAGAAGGCGCGACCACTTTGCTATg < 1:269572/143‑1 (MQ=255) aGTGACCATCGCGCCAGTCGACGTGCGTGTGCTGCTTGGTGTGGCCGGTTTTCAGGCGCGCGCCGCGCTGGCAAATCTCTTTAGCGAACTGCCTTCGAAAGAAAAACAGGTGGTCAAAGTAGGCGCGACCACTTTGCTATGGt < 1:233542/143‑1 (MQ=255) | GCCCATTGCGACGCCAAAGGTAAAATGTGGAGCAATTTACGTCTGTTCCGCGACG‑GCGATGGCTTTGCATGGATTG‑AACGGCGCAGCGTGCGTGAACCGCAGCTGACTGAACTGAAAAAATATGCGGTATTCTCTAAAGTGACCATCGCGCCAGACGACGAGCGTGTGCTGCTTGGTGTTGCCGGTTTTCAGGCGCGCGCCGCGCTGGCAAATCTCTTTAGCGAACTGCCTTCGAAAGAAAAACAGGTAGTCAAAGAAGGCGCGACCACTTTGCTATGGT > NC_000913/3041493‑3041772 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |