Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,156,591 T→G 7.8% intergenic (+73/‑32) yqhD → / → dkgA NADPH‑dependent aldehyde reductase YqhD/methylglyoxal reductase DkgA

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,156,5910TG 7.8% 139.4 / 3.5 55intergenic (+73/‑32)yqhD/dkgANADPH‑dependent aldehyde reductase YqhD/methylglyoxal reductase DkgA
Reads supporting (aligned to +/- strand):  ref base T (27/23);  new base G (3/2);  total (30/25)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.92e-01

CCCAACTGGGCGAAAATCATGACATTACGTTGGATGTCAGCCGCCGTATATACGAAGCCGCCCGCTAAGCTTTTTACGCCTCAAACTTTCGTTTTCGGGCATTTCGTCCAGACTTAAGTTCACAACACCTCACCGGAGCCTGCTCCGGTGAGTTCATATAAAGGAGGAACGTATGGCTAATCCAACCGTTATTAAGCTACAGGATGGCAATGTCATGCCCCAGCTGGGACTGGGCGTCTGGCAAGCAAGTAATGAGGAAGTAATCACCGCCATTCAAAAAG  >  NC_000913/3156451‑3156731
                                                                                                                                            |                                                                                                                                            
cccAACTGGGCGAAAATCATGACATTACGTTGGATGTCAGCCGCCGTATATACGAAGCCGCCCGCTAAGCTTTTTACGCGCTATCCCAGCCTGTTCGGGCATTTCGTCCAGACTTAAGTTCACAACACCTCACCGGAGCCTGc                                                                                                                                            >  1:203864/1‑143 (MQ=255)
    aCTGGGCGAAAATCATGACATTACGTTGGATGTCAGCCGCCGTATATACGAAGCCGCCCGCTAAGCTTTTTACGCCTCAAACTTTCGTTTTCGGGCATTTCGTCCAGACTTAAGTTCACAACACCTCACCGGAGCCTGCTCCg                                                                                                                                        <  1:240583/143‑1 (MQ=255)
      tGGGCGAAAATCATGACATTACGATGGATGTCAGCCGCCGAATATACGAAGCCGCCAGCTAAGCTTTTTACGCCTCAAACTTTCGTTTTCGGGCATTTCGTACAGACTTAAGTTCACAACACCTCAACGGAGCCTGCTCCggc                                                                                                                                      >  1:185813/1‑142 (MQ=255)
      cgggCGAACATCATGACATTACGTTGGATGTCAGCCGCCGTATATACGAAGCCGCCCGCTAAGCTTTTTACGCCTCAAACTTTCGTTTTCGGGCATTTCGTCCAGACTTAAGTTCACAACACCTCACCGGAGCCTGCTCCGGt                                                                                                                                      <  1:196025/142‑1 (MQ=255)
          cGAAAATCATGACATTACATTTGATTTCAGCCGCCGTATATACGAAGCCGCCCGCTAAGCTTTTTACGCCTCAAACTTTCGTTTTCGGGCATTTCGTCCAGACTTAAGTTCACAACACCTCACCGGAGCAGGCTCCGGTGAgg                                                                                                                                  <  1:25232/143‑2 (MQ=255)
              aaTCATGACATTACGTTGGATGTCAGCCGCCGTATATACGAAGCCGCCCGCTAAGCTTTTTACGCCTCAAACTTTCGTTTTCGGGCATTTCGTCCAGACTTAAGTTCACAACACCTCACCGGAGCCTCCTCCGGTGAGTTcct                                                                                                                              >  1:463639/1‑141 (MQ=255)
                gcaggACAGTACGTTGGATGTCAGCCGCCGTATATACGAAGCCGCCCGCTAAGCTTTTTACGCCTCAAACTTTCGTTTTCGGGCATTTCGTCCAGACTTAAGTTCACAACACCTCACCGGAGCCTGCTCCGGTGAGTTCatat                                                                                                                            <  1:345948/139‑1 (MQ=255)
                  aTGACATTACGTTGGATGTCAGCCGCCGTATATACGAAGCCGCCCGCTAAGCTTTTTACGCCTCAAACTTTCGTTTTCGGGCATTTCGTCCAGACTTAAGTTCACAACACCTCACCGGAGCCTGCTCCGGTGAGTTCatatga                                                                                                                          >  1:863599/1‑141 (MQ=255)
                   tGACATTACGTTGGATGTCAGCCGCCGTATATACGAAGCCGCCCGCTAAGCTTTTTACGCCTCAAACTTTCGTTTTCGGGCATTTCGTCCAGACTTAAGTTCACAACACCTCACCGGAGCCTGCTCCGGTGAGTTCATATaaa                                                                                                                         >  1:105198/1‑143 (MQ=255)
                    gACATTGCGTTGGATGTCAGCCGCCGTATATACGAAGCCGCCCGCTAAGCTTTTTACGCCTCAAACTTTCGTTTTCGGGCATTTCGTCCAGACTTAAGTTCACAACACCTCACCGGAGCCTGCTCCGGTGAGTTCATATAAag                                                                                                                        <  1:269269/143‑1 (MQ=255)
                    gACATTACGTTGGATGTCAGCCGCCGTATATACGAAGCCGCCCGCTAAGCTTTTTACGCCTCAAACTTTCGTTTTCGGGCATTTCGTCCAGACTTAAGTTCACAACACCTCACCGGAGCCTGCTCCGGTGAGTTCATATAAag                                                                                                                        <  2:377113/143‑1 (MQ=255)
                      cagtACGTTGGATGTCAGCCGCCGTATATACGAGGCCGCCCGCTAAGCTTTTTACGCCTCAAACTTTCGTTTTCGGGCATTTCGTCCAGACTTAAGTGCACAACACCTCACCGGAGCCTGCTCCGGTGAGTTCATATAAagga                                                                                                                      <  1:345905/140‑1 (MQ=255)
                       aTTACGTTGGATGTCAGCCGCCGTATATACGAAGCCGCCCGCTAAGCTTTTTACGCCTCAAACCTTCGTTTTCGGGCATTTCGGCCAGACTTAAGTTCACAACACCCCCCCGGAGCCTGCCCCGGTGACACCATACAAaggag                                                                                                                     >  2:205390/1‑143 (MQ=255)
                              tGGATGTCAGCCGCCGTATATACGCAGCCGCCCGCTAAGCTTTTTACGCCTCAAACTTTCGTTGTCGGGCACTTCGTCCAGACTCAAGTTCACAACACCTCACCGGAGCCTGCTCCGGTGGGGCCATCTAAAGCACGCACGtc                                                                                                              >  2:514277/1‑142 (MQ=255)
                                     ccgccgccGTATCTCCGAAGTCGCCCGCTAAGCTTTTTACGCCTCAAACTTTCGTTTTCGGGCATTTCGTCCAGACTTAAGTTCACAACACCTCACCGGAGCCTGCTCCGGTGAGTTCATATAAAGGAGGAACGTATGGCTaa                                                                                                       <  1:568046/141‑1 (MQ=255)
                                      aGCCGCCGTATATACGAAGCCGCCCGCTAAGCTTTTTACGCCTCAAACTTTCGTTTTCGGGCATTTCGTCCAGACTTAAGTTCACAACACCTCACCGGAGCCTGCTCCGGTGAGTTCATATAAAGGAGGAACGTATGGCTAAt                                                                                                      >  1:766270/1‑143 (MQ=255)
                                        ccgccgTATATACGAAGCCGCCCGCTAAGCTTTTTACGCCTCAAACTTTCGTTTTCGGGCATTTCGTCCAGACTTAAGTTCACAACACCTCACCGGAGCCTGCTCCGGTGAGTTCATATAAAGGAGGAACGTATGGCTAATcc                                                                                                    >  1:862749/1‑143 (MQ=255)
                                           ccgTATATACGAAGCCGCCCGCTAAGCTTTTTACGCCTCAAACTTTCGTTTTCGGGCATTTCGTCCAGACTTAAGTTCACAACACCTCACCGGAGCCTGCTCCGGTGAGTTCATATAAAGGAGGAACGTATGGCTAATCCAAc                                                                                                 >  1:599148/1‑143 (MQ=255)
                                               atataCGAAGCCACCCGCTAAGCTTTTTACGCCTCAAACTTTCGTTTTCGGGCATTTCGTCCAGACTTAAGTTCACAACACCTCACCGGAGCCTGCTCCGGTGAGTTCATATAAAGGAGGAACGTATGGCTAATCCAACCgct                                                                                             >  1:541712/1‑141 (MQ=255)
                                                  taCGAAGCCGCCCGCTAAGCTTTTTACGCCTCAAACTTTCGTTTTCGGGCATTTCGTCCAGACTTAAGTTCACAACACCTCACCGGAGCCTGCTCCGGTGAGTTCATATAAAGGAGGAACGTATGGCTAATCCAACCGttatt                                                                                          >  1:749683/1‑143 (MQ=255)
                                                   aCGAAGCCGCCCGCTAAGCTTTTTACGCCTCAAACTTTCGTTTTCGGGCATTTCGTCCAGACTTAAGTTCACAACACCTCACCGGAGCCTGCTCCGGTGAGTTCATATAAAGGAGGAACGTATGGCTAATCCAACCGttatta                                                                                         <  1:249588/143‑1 (MQ=255)
                                                   aCGAAGCCGCCCGCTAAGCTTTTTACGCCTCAAACTTCCGTCTTCGGGCATTTCGTCCAGACTTAAGTTCACAACACCTCACCGGAGCCTGCTCCGGTGAGTTCATATAAAGGAGGAACGTATGGCTAATCCAACCGttatta                                                                                         <  2:669222/143‑1 (MQ=255)
                                                    cGATGCCGCCCGCTATGCGTGTTCACCCTCAAACTGTCGTTTTCGGGCATTCCGTCCAGACTCAAGTTCACAACACCTCACCGGAGCCTGCTCCGGTGAGTTCATATAAAGGAGGAACGTATTGCTAATCCAACCGTTATTaa                                                                                        <  2:670686/143‑1 (MQ=255)
                                                    cGAGGCCGCCCGCTAAGCTTTGTACGCCGCCAACTTTCGTTTTCGGGCATTTCGTCCAGACTTAAGTTCACAACACCTCACCGGAGCCTGCTCCGGTGAGTTCATATAAAGGAGGAACGTATGGCTAATCCAACCGTTATTaa                                                                                        <  2:287999/143‑1 (MQ=255)
                                                    cGAAGCCGCCCGCTAAGCTTTTTACGCCTCAAACTTTCGTTTTCGGGCATTTCGTCCGGACTTAAGTTCACAACACCTCACCGGAGCCTGCTCCGGTGa                                                                                                                                    >  1:98319/1‑99 (MQ=255)
                                                    cGAAGCCGCCCGCTAAGCTTTTTACGCCTCAAACTTTCGTTTTCGGGCATTTCGTCCAGACTTAAGTTCACAACACCTCACCGGAGCCTGCTCCGGTGa                                                                                                                                    <  2:98319/99‑1 (MQ=255)
                                                                  aaGCTTTTTACGCCTCAAACTTTCGTTTTCGGGCATTTCGTCCAGACTTAAGTTCACAACACCTCACCGGAGCCTGCTCCGGTGAGTTCATATAAAGGAGGAACGTATGGCTAATCCAACCGTTATTATGCTACAGGATGGCa                                                                          >  1:56388/1‑143 (MQ=255)
                                                                   aGCTTTTTACGCCTCATACCTTCGTTTTCGGGCATTTCGTCCAGACTTAAGTTCACAACACCTCACCGGAGCCTGCTCCGGTGAGTTCATATAAAGGAGGAACGTATGGCTAATCCAACCGTTATTAAGCTACAGGATGGCaa                                                                         <  2:172047/143‑1 (MQ=255)
                                                                             gCCTCGAACTTTCGTTTGCGGGCATTTCGTCCAGACTTAAGTTCACAACACCTCGCCGGAGCCTGCTCGGGTGAGTTCATATAAAGGAGGAACGTATGGCTAATCCAACCGTTATTAAGCTACAGGATGGCAATGTCATGccc                                                               <  2:684611/143‑1 (MQ=255)
                                                                               cTCAAACTTTCGTTTTCGGGCATTTCGTCCAGACTTAAGTTCACAACACCTCACCGGAGCCTGCTCCGGTGAGTTCATATAAAGGAGGAACGTATGGCTAATCCAACCGTTATTAAGCTACAGGATGGCAATGTCATGCCCCa                                                             >  1:272649/1‑143 (MQ=255)
                                                                                tCAAACTTTCGTTTTCGGGCATTTCGTCCAGACTTAAGTTCACAACACCTCACCGGAGCCTGCTCCGGTGCGTTCATATAAAGGAGGAACGTATGGCTAATCCAACCGTTATTCAGCTACCGGCTGGCAATGTCATGCCCCAg                                                            >  1:180052/1‑143 (MQ=255)
                                                                                    aCTTTCGTTGTCGGGCATTTCGTGCAGACTTAAGTTCACACCACCTCGCCGGAGCCTGCTCCGGTGAGTTCATATAAAGGAGGAACGTATGGCTAATCCAACCGTTATTAAGCTACAGGATGGCAATGTCATGCCCCAGCTgg                                                        <  2:138544/143‑1 (MQ=255)
                                                                                      tttCGTTTTCGGGCATTTCGTCCAGACTTAAGTTCACAACACCTCACCGGAGCCTGCTCCGGTGAGTTCATATAAAGGAGGAACGTATGGCTAATCCAACCGTTATTAAGCTACAGGATGGCAATGTCATGCCCCAGCTGGGa                                                      <  2:766270/143‑1 (MQ=255)
                                                                                          gTTTTCGGGCATTTCGTCCAGACTTAAGTTCACAACCCCTCACCGGAGCCTGCTCCGGTGAGTACATCTAAAGCACGAACCTAAGGCTAAGCCAACCGTTATTAAGCTACAGGATGGCAATGTCATGCCCCAGCTGGGACTgg                                                  <  1:414456/143‑1 (MQ=255)
                                                                                           ttttCGGGCATTTCGTCCAGACTTAAGTTCACAACACCTCACCGGCGCCTGCTCCGGTGAGTTCATATAAAGGAGGCGCGTATGGCTAATCCACCCGCTATTACGCGACAGGCTGGCAAGGGCAACCCCCAGCTAGGGCTgag                                                 >  2:882746/1‑141 (MQ=255)
                                                                                           ttttCGGGCATTTCGTCCAGACTTAAGTTCACAACACCTCACCGGAGCCTGCTCCGGTGAGTTCATATAAAGGAGGAACGTATGGCTAATCCAACCGTTATTAAGCTACAGGATGGCAATGTCATGCCCCAGCTGGGACTggg                                                 >  1:608196/1‑143 (MQ=255)
                                                                                                gggCAGTTGGGCCAGACGTAAGTTCACACCACCTCACCGGGGCCTGCTCCGGTGAGTTCATATGAAGGAGGAACGTATGGCTAATCCAACCGTTATTAAGCTACAGGATGGCAATGTCATGCCCCAGCTGGGACTGGGCGTCt                                            <  1:748869/143‑1 (MQ=255)
                                                                                                 cgcacctCGTCCAGACTTAAGTCCACAACACCGCACCGGAGCCTGCCCCGGTGAGTTCATATAAAGGAGGAACGTATGGCTAATCCAACCGTTATTAAGCTACAGGATGGCAATGTCATGCCCCAGCTGGGACTGGGCGTCTg                                           <  2:522191/137‑1 (MQ=255)
                                                                                                     tttCGTCCAGACTTAAGTTCACAACACCTCACCGGAGCCTGCTCCGGTGAGTTCATATAAAGGAGGAACGTATGGCTAATCCAACCGTTATTAAGCTACAGGATGGCAATGTCATGCCCCAGCTGGGACTGGGCGTCTGgcaa                                       >  1:895730/1‑143 (MQ=255)
                                                                                                     tttCGTCCAGACTTAAGTTCACAACACCTCACCGGAGCCTGCTCCGGTGAGTTCATATAAAGGAGGAACGTATGGCTAATCCAACCGTTATTAAGCTACAGGATGGCAATGTCATGCCCCAGCTGGGACTGGGCGTCTGgcaa                                       >  2:786503/1‑143 (MQ=255)
                                                                                                             aGACTTAAGTTCACAACACCTCACCGGAGCCTGCTCCGGTGAGTTCATATAAAGGAGCAACGTATGGCTAATCCAACCGTTATTAAGCTACAGGAGGGCGATGGCATACCCCCGCTGGGAAGGGGCGGCTGGCAAGCGAGTaa                               >  2:43760/1‑143 (MQ=255)
                                                                                                              gACTTAAGTTCACAACACCGCACCGGAGCCTGCTCCGGTGAGTTCATATAAAGGAGGAACGTATGGCTAATCCAACCGTTATTAAGCTACAGGATGGCAATGTCATGCCCCAGCTGGGACTGGGCGTCTGGCAAGCAAGTAAt                              <  2:463639/143‑1 (MQ=255)
                                                                                                                   aacttCAAAACCCCGCGCCGGCGCCGGTGCCGCTGAGTTCATATAAAGGAGGAACTTATGGCTAATCCAACCGTTATTAAGCGACAGGATGGCAATGTCATGCCCCAGCTGGGACTGGGCGTCTGGCAAGCAAGTAATGAGGa                         <  2:341749/140‑1 (MQ=255)
                                                                                                                   aaGTTCACAACACCTCACCGGAGCCTGCTCCGGTGAGTTCATATAAAGGAGGAACGTATGGCTAATCCAACCGTTATTAAGCTACAGGATGGCAATGTCATGCCCCAGCTGGGACTGGGCGTCTGGCAAGCAAGTAATGAGGa                         <  1:372541/143‑1 (MQ=255)
                                                                                                                      ttCACAACACCTCACCGGAGCCTGCTCCGGTGAGTTCATATAAAGGAGGAACGTATGGCTAATCCAACCGTTATTAAGCTACAGGATGGCAATGTCATGCCCCAGCTGGGACTGGGCGTCTGGCAAGCAAGTAATGAGGAAGt                      >  1:845331/1‑143 (MQ=255)
                                                                                                                                cTCACCGGAGCCTGCTCCGGTGAGTTCATATAAAGGAGGAACGTATGGCTAATCCAACCGTTATTAAGCTACAGGATGGCAATGTCATGCCCCAGCTGGGACTGGGCGGCTGGCAAGCAACGAATGAGGAAGTAATGACCGcc            >  2:650262/1‑143 (MQ=255)
                                                                                                                                 tCACCGGAGCCTGCTCCGGGGAGTTCCTAAACAGGAGGAACGTATGGCTAATCCAACCGTTATTAAGCTACAGGATGGCGATGTCATGCCCCAGCTGGGACTGGGCGTCTGGCAAGCAAGTAATGAGGAAGTAATCACCGCCa           <  1:64346/143‑1 (MQ=255)
                                                                                                                                 tCACCGGAGCAGGCTCCGGTGAGTTCCTCTAAAGGAGGAACGTATGGCTAATCCAACCGTTATTACGCTACAGGATGGCAATGTCATGCCCCAGCTGGGACTGGGCGTCTGGCAAGCGAGGAATGAGGAAGGAGTCACCGCCa           >  1:76921/1‑143 (MQ=255)
                                                                                                                                   aCCGTAGCCTGCCCAGGTTAGTTCATATAAAGGAGGAACGTATGGCTAATCCAACCGTTATTAAGCTACAGGATGGCAATGTCATGCCCCAGCTGGGACTGGGCGTCTGGCAAGCAAGTAATGAGGAAGTAATCACCGCCAtt         <  1:446262/143‑1 (MQ=255)
                                                                                                                                   aCCGGAGCAGGCTCCGGTGAGTTCATATAAAGGAGGAACGTATGGCTAATCCAACCGTTATTAAGCTACAGGATGGCAATGTCATGCCCCAGCTGGGACTGGGCGTCTGGCAAGCACGTAATGAAGAATTAATCACCGCCAtt         >  2:215619/1‑143 (MQ=255)
                                                                                                                                   aCCGGAGCAGGCTCCGGTGAGTTCATATAAAGGAGGAACGTATGGCTAATCCAACCGTTATTAAGCTACAGGATGGCAATGTCATGCCCCAGCTGGGACTGGGCGTCTGGCAAGCAAGTAATGAGGAAGTAATCACCGCCAtt         >  1:541007/1‑143 (MQ=255)
                                                                                                                                        aGCCTGCTCCGGTGAGTTCATATAAAGGAGGAACGTATGGCTAATCCAACCGTTATTAAGCTACAGGATGGCAATGTCATGCCCCAGCTGGGACTGGGCGTCTGGCAAGCAAGTAATGAGGAAGTAATCCCCGCCATTCcaaa    >  2:365028/1‑143 (MQ=255)
                                                                                                                                          ccTGCTCCGGTGAGTTCATATAAAGGAGGAACGTATGGCTAATCCAACCGTTATTAAGCTACAGGATGGCAATGTCATGCCCCAGCTGGGACTGGGCGTCTGGCAAGCAAGTAATGCGGAAGTACTCACCGCCATTCAAAAAg  >  1:606684/1‑143 (MQ=255)
                                                                                                                                          ccTGCTCCGGTGAGTTCATATAAAGGAGGAACGTATGGCTAATCCAACCGTTATTAAGCTACAGGATGGCAATGTCATGCCCCAGCTGGGACTGGGCGTCTGGCAAGCAAGTAATGAGGAAGTAATCACCGCCATTCAACAAg  >  1:460465/1‑143 (MQ=255)
                                                                                                                                          ccTGCTCCGGTGAGTTCATATAAAGGAGGAACGTATGGCTAATCCAACCGTTATTAAGCTACAGGATGGCAATGTCATGCCCCAGCTGGGACTGGGCGTCTGGCAAGCAAGTAATGAGGAAGTAATCACCGCCATCCAAAAAg  >  2:166979/1‑143 (MQ=255)
                                                                                                                                            |                                                                                                                                            
CCCAACTGGGCGAAAATCATGACATTACGTTGGATGTCAGCCGCCGTATATACGAAGCCGCCCGCTAAGCTTTTTACGCCTCAAACTTTCGTTTTCGGGCATTTCGTCCAGACTTAAGTTCACAACACCTCACCGGAGCCTGCTCCGGTGAGTTCATATAAAGGAGGAACGTATGGCTAATCCAACCGTTATTAAGCTACAGGATGGCAATGTCATGCCCCAGCTGGGACTGGGCGTCTGGCAAGCAAGTAATGAGGAAGTAATCACCGCCATTCAAAAAG  >  NC_000913/3156451‑3156731

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑