| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_000913 | 151,519 | A→C | 12.4% | I27M (ATT→ATG) | yadK ← | putative fimbrial protein YadK |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 151,519 | 0 | A | C | 12.4% | 124.5 / 13.6 | 58 | I27M (ATT→ATG) | yadK | putative fimbrial protein YadK |
| Reads supporting (aligned to +/- strand): ref base A (28/22); new base C (3/4); total (31/27) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 6.91e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 2.13e-02 | |||||||||||
GTTGATAACTTTCAGGAGTAACATTACCTGAAAAATATCCCACCCCAACGACGCCAAGATCAATATTACCCTGGTTACTGATTCCGCTTTTGCAGGTGCTGTTTTTTACATTGGCAACAAGGTCAATATCCTGTCCAGCAATGGCTGGACAGGCGATGCAAAACAGTAATGACAGAAACAGAATTATTCTCTTCATCAGCTTACGTTGAGTGGGATGCATATATAGTTCCTGAATCTGTAAGTCTGTTAGTTATAGGTTACTGTAAATGTGGCTGGTGCAG > NC_000913/151380‑151660 | gTTGATAACTTTCAGGAGTAACATTACCTGAAAAATATCCCACCCCAACGACGCCAAGATCAATATTACCCTGGTTACTGATTCCGCTTTTGCAGGTGCTGTTTTTTACATTGGCAACAAGGTCAATATCCTGTCCAGCAATg > 1:753975/1‑143 (MQ=255) ttaTAACTGTCAGCAGTAACATTACCTGAAAAATATCCCACCCCATCGACGCCAAGATCAATATTACCCTGGTTACTGATTCCGCGTTTGCAGGTGCTGTTTTTTACATTGGCAACAAGGTCAATATCCTGTCCAGCAATGGc < 2:318111/141‑1 (MQ=255) gATACCTTTCAGGAGTAACATTACCTGAAAAATATCCCACCCCAACGACGCCAAGATCAATATTACCCTGGTTACTGATTCCGCTTTTGCAGGTGCTGTTTTTTACATTGGCAACAAGGTCAATATCCTGTCCAGCAATGGCt < 2:202326/143‑1 (MQ=255) ccttCAGGAGTAACACTACCTTAAAAATATCCCCCCCTAACGACGCCAACATCAATATTACCCTGGTTACTGATTCCGCTTTTTCAGGTGCTGTTTTTTACATTGGCAACAAGGTCAATATCCTGTCCAGCCATTGCTGGACa < 1:789277/141‑1 (MQ=255) cctgcAGGAGTAACATTACCTGAAATATATCCCACCCCAACGACGCCAAGATCAATATTACCCTGGTTACTGATTCCGCTTTTGCAGGTGCTGTTTTTTACATTGGCAACAAGGTCAATATCCTGTCCAGCCATTGCTGGACa < 2:152826/139‑1 (MQ=255) cTTTCAGGAGTAACATTACCTGAAAAATATCCCACCCCAACGACGCCAAGATCAATATTACCCTGGTTACTGATTCCGCTTTTGCAGGTGCTGTTTTTTACATTGGCAACAAGGTCAATATCCTGTCCAGCCATTGCTGGACa < 1:591174/143‑1 (MQ=255) cTTTCAGGAGTAACATTACCTGAAAAATATCCCACCCCAACGACGCCAAGATCAATATTACCCTGGTTACTGATTCCGCTTTTGCAGGTGCTGTTTTTTACATTGGCAACAAGGTCAATATCCTGTCCAGCCATTGCTGGACa < 2:69320/143‑1 (MQ=255) aGGAGTAACATTACCTGAAAAATATCCCACCCCAACGACGCCAAGATCAATATTACCCTGGTTACTGATTCCGCTTTTGCAGGTGCTGTTTTTTACATTGGCAACAAGGTCAATATCCTGTCCAGCAATGGCTGGACAGGCGa < 2:549128/143‑1 (MQ=255) ggAGTTACAGCACCTGAAAAATATCCCACCCCAGCGACGCCAAGAGCAATATTACCCTGGTTACTGATTCCGCTTTTGCAGGTGCTGTTTTTTACATTGGCAACAAGGTCAATATCCTGTCCAGCAATGGCTGGACAGGCGAt < 2:251258/143‑1 (MQ=255) gTAACATTACCTGAAAAATATCCCACCCCAACGACGCCAAGATCAATATTACCCTGGTTACTGATTCCGCTTTTGCAGGTGCTGTTTTTTACATTGGCAACAAGGTCAATATCCTGTCCAGCAACGGCTGGACAGGCGATGCa > 1:39991/1‑143 (MQ=255) tcaCATTACCTGAAAAATATCCCACCCCAACGACGCCAAGATCAATATTACCCTGGTTACTGATTCCGCTTTTGCAGGTGCTGTTTTTTACATTGGCAACAAGGTCAATATCCTGTCCAGCAATGGCTGGACAGGCGATGCaa < 2:236247/141‑1 (MQ=255) cATTACCTGAAAAATATCCCACCCCAACGACGCCAAGATCAATATTACCCTGGTTACTGATTCCGCTTTTGCAGGTGCTGTTTTTTACATTGGCAACAAGGTCAATATCCTGTCCTGCAATGGCTGGACAGGCGATGCAAAAc > 2:422153/1‑143 (MQ=255) attttcTGAAAAATAACCAACCCCAACGAGGCCAAGATCAAGATTACCCTGGTTACTGATTCCGCTTTTGCAGGTGCTGTTTTTTACATTGGCAACAAGGTCCATATCCTGTCCAGCAATGGCTGGACAGGCGATGCAAAACa < 1:350387/138‑1 (MQ=255) tGAAAAATATCCCACCCCAACGACGCCAAGATCAATATTATGCTGGTTACTGATTCCGCTTTTGCAGGTGCTGTGTTTTACATTGGCCACAAGGTCGATATCCTGTCCAGCAACGGCTGGACAGGCGATGCAAAACAGTAATg > 2:637433/1‑143 (MQ=255) aaaaaTATCCCACCCCAACGACGCCAAGATCAATATTACCCTGGTTACTGATTCCGCTTTTGCAGGTGCTGTTTTTTACATTGGCAACAAGGTCAATATCCTGTCCAGCAATGGCTGGACAGGCGATGCAAAACAGTAATGac < 2:889843/143‑1 (MQ=255) aaaTATCCCACCCCAACGACGCCAAGATCAATATTACCCTGGCTACTGATTCCGCTTTTGCAGGTGCTGTTTTTTACATTGGCAACAAGGTCAATATCCTGTCCAGCAATGGCTGGACAGGCGCTGCAAAACAGTAATGacag > 2:806962/1‑143 (MQ=255) ccccAACTACGCCAAGATCAATATTACCCTGTTTACTGATTCCGCTTTTGCTGGTGTTGTTTTTTATTTTGTCAACAAGGTCAATAGCCTGGCGAGCAATGGCTGGACAGGCGATGCAAAACAGTAATGACAGAAACAGAatt < 1:621386/143‑1 (MQ=255) cccAACGACGCCAAGATCAATATTACCCTGGTTACTGATTCCGCTTTTGCAGGTGCTGTTTTTTACATTGGCAACAAGGTCAATATCCTGTCCAGCAATGGCTGGACAGGCGATGCAAAACAGTAATGACAGAAACAGAatta > 1:745132/1‑143 (MQ=255) aaCGACGCCAAGATCAATATTACCCTGGTTACTGATTCCGCTTTTGCAGGTGCTGTTTTTTACATTGGCAACAAGGTCAATATCCTGTCCAGCAATGGCTGGACAGGCCATGCAAAACAGTAATGACAGCAACAGAATTATtc > 1:692691/1‑143 (MQ=255) gacgCCAAGATCAATATTACCCTGGTTACTGATTCCGCTTTTGCAGGTGCTGTTTTTTACATTGGCAACAAGGTCAATATCCTGTCCAGCAATGGCTGGACAGGCGATGCAAAACAGTAATGACAGAAACAGAATTATTctct > 2:415864/1‑143 (MQ=255) cAAGATCAATATTACCCTGGTTACTGATTCCGCTTTTGCAGGTGCTGTTTTTTACATTGGCAACAAGGTCAATATCCTGTCCAGCAATGGCTGGACAGGCGATGCAAAACAGTAATGACAGAAACAGAATTATTCTCTtcatc > 2:161498/1‑143 (MQ=255) aTCAATATTACCCTGGTTACTGATTCCGCTTTTGCAGGTGCTGTTTTTTACATTGGCAACAAGGTCAATATCCTGTCCAGCAATGGCTGGACAGGCGATGCAAAACAGTAATGCCAGAAACAGAATTATTCTCTTCATCAGCt > 1:21494/1‑143 (MQ=255) aaTATTACCCTGGTTACTGATTCCGCTTTTGCAGGTGCTGTTTTTTACATTGGCAACAAGGTCAATATCCTGTCCAGCAATGGCTGGACAGGCGATGCAAAACAGTAATGACCGACACCGCATTATTCTCTTCATCAGCttgc > 1:281005/1‑141 (MQ=255) cccTGGTTACTGATTCCGCTTTTGCAGGTGCTGTTTTTTACATTGGCAACAAGGTCAATATCCTGTCCAGCAATGGCTGGACAGGCGATGCAAAACAGTAATGACAGAAACAGAATTATTCTATTCATTAGCTTCCGTTGAGt > 2:880831/1‑143 (MQ=255) ccTGGTTACTGATTCCGCTTTTGCAGGTGCTGTTTTTTACATTGGCAACAAGGTCAATATCCTGTCCAGCAATGGCTGGACAGGCGATGCAAAACAGTAATGACAGAAACAGAATTATTCTCTTCATCAGCTTACGTTGAGTg > 1:309361/1‑143 (MQ=255) ttACTGATTCCGCTTTTGCAGGTGCTGTTTTTTACATTGGCAACAAGGTCAATATCCTGTCCAGCAATGGCTGGACAGGCGATGCAAAACAGTAATGCCAGAAACAGAATTATTCTCTTCATCAGCTTACGTTGAGTGGGATg < 1:702435/143‑1 (MQ=255) aCTGATTCCGCTTTTGCAGGTGCTGTTTTTTACATTGGCAACAAGGTCAATATCCTGTCGAGCAATGGCTGGACAGGCGATGCATAACAGTAATGACAGAAACAGAATTATTCTCTTCATCAGCTTACGTTGAGTGGGATGCa < 2:444121/143‑1 (MQ=255) aCTGATTCCGCTTTTGCAGGTGCTGTTTTTTACATTGGCAACAAGATCAATATCCTGTCCAGCAATGGCTGGACAGGCGATGCAAACCAGTCATGCCAGAAACAGCATTATTCTCTTCATCAGCTTACGTTGAGTCGGCTGCa > 1:781471/1‑143 (MQ=255) aCTGATTCCGCTTTTGCAGGTGCTGCTGTTTACATTGGCAACAAGGTCAATATCCTGTCCAGCAATGGCTGGACAGGCGATGCAAAACAGTAATGACAGAAACAGAATTATTCTCTTCATCAGCTTACGTTGAGTGGGATGCa < 2:644761/143‑1 (MQ=255) tGATTCCGCTTTTGCAGGTGCTGTTTTTTACATTGGCAACAAGGTCACTATCCTGTCCAGCAATGGCTGGACAGGCGGTGCAAAACAGTAATGACAGAAACAAAATTATTCTCTTCATCAGCTTACGTTGGGTGGGATGCata > 1:819385/1‑143 (MQ=255) aTTCCGCTTTTGCAGGTGCTGTTTTTTACATTGGCAACAAGGTCAATATCCTGTCCAGCAATGGCTGGACAGGCGATGCAAAACAGTAATGACAGAAACAGAATTATTCTCTTCATCAGCTTACGTTGAGGGGGATGCagata > 2:178790/1‑143 (MQ=255) gCTTTTGCAGGTGCTGTTTTTTACATTGGCAACAAGGTCAATATCCTGTCCAGCAATGGCTGGACAGGCGATGCAAAACAGTAATGACAGAAACAGAATTATTCTCTTCATCAGCTTACGTTGAGTGGGATGCATCTATAGtt > 1:623924/1‑143 (MQ=255) cTTTTGCAGGTGCTGTTTTTTACATTGGCAACAAGGTCAATATCCTGTCCAGCAATGGCTGGACAGGCGATGCAAAACAGTAATGACAGAAACAGAATTATTCTCTTCATCAGCTTACGTTGAGTGGGATGCATATATAGTTc < 1:149401/143‑1 (MQ=255) ttttGCAGGTGCGGTTGTCTACGTTGGCAACAAGGTCAATATCCTGTCCAGCAATGGCTGGACAGGCGATGCAAAACAGTAATGACAGAAACAGAATTATTCTCTTCATCAGCTTACGTTGAGTGGGATGCATATATAGTTcc < 1:26344/143‑1 (MQ=255) ctttGCAGGTGCTGCGTTTTACATTGGCAACAAGGTCAATATCCTGTCCAGCAATGGCTGGACAGGCGATGCAAAACAGTAATGACAGAAACAGAATTATTCTCTTCATCAGCTTACGTTGAGTGGGATGCATATATAGTTcc < 1:361316/142‑1 (MQ=255) tttGCAGGTGCTGTTTTTTACATTGGCAACACGGTCAATATCCTGTCCAGCAATGGCTGGACAGGCGACGCAAAACAGTAATGACAGAAACAGAATTATTCTCTTCACCCGCTACCGCTGAGTGGGAGCCATATCTAGTTCCt > 2:132552/1‑143 (MQ=255) cAGGTGCTGTTTTTTACATTGGCAACAAGGTCAATATCCTGTCCAGCAATGGCTGGACAGGCGATGCAAAACAGTAATGACAGAAACAGAATTATTCTCTTCATCAGCTTACGTTGAGTGGGATGCATATATAGTTCCTGCAt > 2:815750/1‑143 (MQ=255) cAGGTGCTGTTTTTTACATTGGCAACAAGGTCAATATCCTGTCCAGCAATGGCTGGACAGGCGATGCAAAACAGTAATGACAGAAACAGAATTATTCTCTTCATCAGCTTACGTTGAGTGGGATGCATATATAGTTCCTGAAt < 2:694658/143‑1 (MQ=255) cAGGTGCTGTTTTTTACATTGGCAACAAGGTCAATATCCTGTCCAGCAATGGCTGGACAGGCGATGCAAAACAGTAATGACAGAAACAGAATTATTCTCTTCATCAGCTTACGTTGAGTGGGATGCATATATAGTTCCTGAAt > 1:532980/1‑143 (MQ=255) gcggTTTTTTACATTGGCAACAAGGTCAATATCCTGTCCAGCAATGGCTGGACAGGCGATGCAAAACAGTAATGACAGAAACAGAATTATTCTCTTCATCAGCTTACGTTGAGTGGGATGCATATATAGTTCCTGAATCTGTa < 2:667581/140‑1 (MQ=255) cTGTTTTTTACATTGGCAACAAGGTCAATATCCTGTCCAGCAATGGCTGGACAGGCGATGCAAAACAGTAATGACAGAAACAGAATTATTCTCTTCATCAGCTTACGTTGAGTGGGATGCATATATAGTTCCTGAATCTGTaa < 1:247472/143‑1 (MQ=255) ttttttACATTGGCAACAAGGTCAATATCCTGTCCAGCAATGGCTGGACAGGCGATGCAACACAGTAATGACAGAAACAGAATTATTCTCTTCATGAGCTTCCGCTGAGTACGCTGCAAATATAGTTCCTGAATCCGTAAGTc > 2:630735/1‑143 (MQ=255) ttttttACATTGGCAACAAGGTCAATATCCTGTCCAGCAATGGCTGGACAGGCGATGCAAAACAGTAATGACAGAAACAGAATTATTCTCTTCATCAGCTTACGTTGAGTGGGATGCATATATAGTTCCTGAATCTGTAAGTc > 1:822668/1‑143 (MQ=255) cATTGGCAACAAGGTCAATATCCTGTCCAGCAATGGCTGGACAGGCGATGCAAAACAGTAATGACAGAAACAGAACTATTCTCTTCATCAGCTTACGTTGAGTGGGATGCATATATAGTTCCTGAATCTGTAAGTCTgttagt < 2:447944/143‑1 (MQ=255) ttGGCAACAAGGTCAATATCCTGTCCAGCAATGGCTGGACAGGCGATGCAAAACAGTAATGACAGAAACAGAATTATTCTCTTCATCAGCTTACGTTGAGTGGGATGCATATATAGTTCCTGAATCTGTAAGTCTgttcgtta > 1:154906/1‑143 (MQ=255) ttGGCAACAAGGTCAATATCCTGTCCAGCAATGGCTGGACAGGCGATGCAAAACAGTAATGACAGAAACAGAATGATTCTCTTCATCAGCTTACGTAGAGTGGGATGCATATATAGTTCCTGAATCTGTAAGtgtgacagtga > 2:735100/1‑136 (MQ=255) ggCAACAAGGTCAATTTCCTGCCCAGCAATGGCGTGACAGGCGATCCAAAACAGTAATGACAGAAACAGAATTATTCTCTGCATCAGCTTACGTTGAGTTGGATGCATATATAGTTCCTGAATCTGTAAGTCTCTTAGTtata < 2:714815/143‑1 (MQ=255) gCAACAAGGTCAATATCCTGTCCAGCAATGGCTGGACAGGCGATGCAAAACAGTAATGACAGAAACAGAATTATTCTCTTCATCAGCTTACGTTGAGTGGGATGCATATTTCGTTCCTGAATCTGTAAGTCTGTTAGTTATAg < 1:89890/143‑1 (MQ=255) caaGGTCAATATCCTGTCCAGCAATGGCTGGACAGGCGATGCAAAACAGTAATGACAGAAACAGAATTATTCTCTTCATCAGCTTACGTTGAGTGGGATGCATATATAGTTCCTGAATCTGTAAGTCTGTTAGTTATAGGTTa < 1:454732/143‑1 (MQ=255) aaGGTCAATATCCTGTCCAGCAATGGCTGGACAGGCGATGCAAAACAGTAATGACAGAAACAGAATTATTCTCTTCATCAGCTTACGTTGAGTGGGATGCATATATAGTTCCTGAATCTGTAAGTCTGTTAGTTATAGGTTAc > 1:205428/1‑143 (MQ=255) gTCAATATCCTGTCCAGCAATGGCTGGACAGGCGATGCAAAACAGTAATGACAGAAACAGAATTATTCTCTTCATCAGCTTACGTTGAGTGGGATGCATATATAGTTCCTGAAGCTGTAAGTCTGTTAGGTATAGGTTACTGt > 2:174220/1‑143 (MQ=255) aaTATCCTGTCCAGCAAAGGCTGGACAGGCGATGCAAAACAGTAATGACAGAAACAGAATTATTCTCTTCATCAGCTTACGTTGAGTGGGATGCATATATAGTTCCTGAATCTGTAAGTCTGTTAGTTATAGGTTACTGTaaa < 1:457762/143‑1 (MQ=255) tcgccTGTCCAGCCATTGCTGGACAGGCGATGCAAAACAGTAATGACAGAAACAGAATTATTCTCTTCATCAGCTTACGTTGAGTGGGATGCATATATAGTTCCTGAATCTGTAAGTCTGTTAGTTATAGGTTACTGTAAAtg > 2:205060/4‑143 (MQ=255) ccTGTCCTGCGATTGCTGGACAGGCGATGCAAAACAGTAATGACAGAAACAGAATTATTCTCTTCATCAGCTTACGTTGAGTGGGATGCATATATAGTTCCTGACTCTGTAAGTCTGTTAGTTATAGGTTACTGTAAATGTgg < 2:109230/143‑1 (MQ=255) ccTGTCCAGCAATGGCTGGACAGGCGATGCAAAACAGTAATGACAGAAACAGAATTATTCTCTTCATCAGCTTACGTTGAGTGGGATGCATATATAGTTCCTGAATCTGTAAGTCTGTTAGTTATAGGTTACTGTAAATGTgg > 1:770590/1‑143 (MQ=255) cTGTCCAGCCATTGCTGGACAGGCGATGCAAAACAGTAATGACAGAAACAGAATTATTCTCTTCATCAGCTTACGTTGAGTGGGATGCATATATAGTTCCTGAATCTGTAAGTCTGTTAGCTTTAGGTTACTGTCAATTTGGc > 1:142238/1‑143 (MQ=255) tGTCCAGCCATTGCTGGACAGGCGATGCAAAACAGTAATGACAGAAACAGAATTATTCTCTTCATCAGCTTACGTTGAGTGGGATGCATATATAGTTCCTGAATCTGTAAGTCTGTTAGTTATAGGTTACTGTAAATGTGGCt > 1:477345/1‑143 (MQ=255) cAATGGCTGGACAGGCGATGCAAAACAGTAATGACAGAAACAGAATTATTCTCTTCATCAGCTTACGTTGAGGGGGATGCATATATAGTTCCTGAATCTGTAAGTCTGTTAGTTATAGGTTACTGTAAATGTGGCTGGTGCAg > 1:69440/1‑143 (MQ=255) | GTTGATAACTTTCAGGAGTAACATTACCTGAAAAATATCCCACCCCAACGACGCCAAGATCAATATTACCCTGGTTACTGATTCCGCTTTTGCAGGTGCTGTTTTTTACATTGGCAACAAGGTCAATATCCTGTCCAGCAATGGCTGGACAGGCGATGCAAAACAGTAATGACAGAAACAGAATTATTCTCTTCATCAGCTTACGTTGAGTGGGATGCATATATAGTTCCTGAATCTGTAAGTCTGTTAGTTATAGGTTACTGTAAATGTGGCTGGTGCAG > NC_000913/151380‑151660 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |