Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,332,823 T→C 11.7% intergenic (‑88/+39) yhbE ← / ← rpmA inner membrane protein YhbE/50S ribosomal subunit protein L27

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,332,8230TC11.7% 108.2 / 6.2 50intergenic (‑88/+39)yhbE/rpmAinner membrane protein YhbE/50S ribosomal subunit protein L27
Reads supporting (aligned to +/- strand):  ref base T (15/28);  new base C (3/3);  total (19/31)
Fisher's exact test for biased strand distribution p-value = 6.56e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.01e-01

AGCAAATTGCTGTGGTGAGCGCCAAAAGAATGCCAATGCCTGCCTGCTGCTTCATACCGTATTCCCTGCAAAGCGCATTTGCGGATTAACGTCGCACCATGCGCATTTTTACCGGTTATCGAATGTAAAAAGCCCCGCAACGTGTTGCGGGGCTTTCATCCGTTACCGGGACGCGAAAAACTTATTCAGCTTCGATGCTGATAAATTTACGGTTTTTCGGGCCTTTAACTTCGAATTTCACTTTACCGTCTGCTTTAGCAAACAGAGTGTGGTCACGACCGC  >  NC_000913/3332681‑3332962
                                                                                                                                              |                                                                                                                                           
aGCAAATTGCTGTGGTGAGCACCAAAAGAATGACAATGCCTGCCTGCTGCTTGATACCGTATTCCCTGCAAAGCGCAGTTGCGGATTAACGTCGCACCATGCGCAGTTTCGCCGGTTACCGAACATAAACAGCCGCGCAGCgt                                                                                                                                             >  2:466016/1‑143 (MQ=255)
 gCAAATTGCTGTGGTGAGCGCCAAGAGAATGCCAATGCCTGCCTGCTGCTTCATACCGTATTCCCTGCAAAGCGCATTTGCGGATTAACGTCGCACCATGCGCATTTTTACCGGTTATCGAATGTAAAAAGCCCCGCAACgtg                                                                                                                                            <  2:565855/143‑1 (MQ=255)
 gCAAATTGCTGTGGTGAGCGCCAAAAGAATGCCAATGCCTGCCTGCTGCTTCATACCGTATTCCCTGCAAAGCGCATTTGCGGATTAACGTCGCACCATGCGCATTTTTACCGGTTATCGAATGTAAAAAGCCCCGCAACgtg                                                                                                                                            <  2:767328/143‑1 (MQ=255)
      ttGCTGTGGTGAGCGCCAAAAGAATGCCAATGCCTGCCTGCTGCTTCATACCGTATTCCCTGCAAAGCGCATTTGCGGATTAACGTCGCACCATGCGCATTTTTACCGGTTATCGAATGTAAAAAGCCCCGCAACGTGTTGCg                                                                                                                                       >  1:795038/1‑143 (MQ=255)
      taccTGTGGTGAGCGCCAAAAGAATGCCAATGCCTGCCTGCTGCTTCATACCGTATTCCCTGCAAAGCGCATTTGCGGATTAACGTCGCACCATGCGCATTTTTACCGGTTATCGAATGTAAAAAGCCCCGCAACGTGTTGCg                                                                                                                                       <  1:194079/140‑1 (MQ=255)
       tGCTGTGGTGAGCGCCAAAAGAATGCCAATGCCTGCCTGCTGCTTCATACCGTATTCCCTGCAAAGCGCATTTGCGGATTAACGTCGCACCATGCGCATTTTTACCGGTTATCGAATGTAAAAAGCCCCGCAACACGTTGCgg                                                                                                                                      <  2:772261/143‑1 (MQ=255)
       tGCTGTGGTGAGCGCCAAAAGAATGCCAATGCCTGCCTGCTGCTTCATACCGTATTCCCTGCAAAGCGCATTTGCGGATTAACGTCGCACCATGCGCATTTTTACCGGCTATCGAATGTAAAAAGCACCGCAACGAGTTGCgc                                                                                                                                      >  2:469775/1‑142 (MQ=255)
         cTGGGGTGAGCGCCAAAAGAATGCCAATGCCTGCCTGCTGCTTCATACCGTATTCCCTGCAAAGCGCATTTGCGGATTAACGTCGCACCATGCGCATTTTTACCGGTTATCGAATGTAAAAAGCCCCGCAACACGTTGCgggg                                                                                                                                    <  1:28148/143‑1 (MQ=255)
           gaggtgCGCCCCACAAGGATGCCAATGCCTGCCTGCTGCTTCATACCGTATTCCCTGCAAAGCGCATTTGCGGATTAACGTCGCACCATGCGCATTTTTACCGGTTATCGAATGTAAAAAGCCCCGCAACGTGTTGCGGGGCt                                                                                                                                  <  2:422206/141‑1 (MQ=255)
              gcgAGCGCCTCATGGATGCCAGTGCCTGCCTGCTGCTTCATCCCGTATTCCCTGCAAAGCGCATTTGCGGATTAACGTCGCACCATGCGCATTTTTTCCGGTTATCGAATGTAAAAAGCCCCGCAACACGTTGCGGGGCtttt                                                                                                                               <  2:48692/141‑2 (MQ=255)
               tgAGCGCCAAAAGAATGCCAATGCCTGCCTGCTGCTTCATACCGTATTCCCTGCAAAGCGCATTTGCGGATTAACGTCGCACCATGCGCATTTTTACCGGTTATCGAATGTAAAAAGCCCCGCAACGCGTTGCGGGGCTTTCa                                                                                                                              >  1:324447/1‑143 (MQ=255)
                  gcgcCAAAAGAATGCCAATGCCTGTTTGTTGTTTTATATCGTATTCCCTGCAAAGCGCATTGGCGGATGAACGGCGCACCATGCGCATTTTTACCGGTTATCGAATGTAAAAAGCCCCGCAACGTGTTGCGGGGCTTTCATcc                                                                                                                           <  1:555405/143‑1 (MQ=255)
                    gcCAAAAGAATGCCAATGCCTGCCTGCTGCTTCATACCGTATTCCCTGCAAAGCGCATTTGCGGATTAACGTCGCACCATGCGCATTTTTACCGGTTATCGAATGTAAAAAGCCCCGCAACGTGTTGCGGGGCTTTCATCCGt                                                                                                                         <  2:877921/143‑1 (MQ=255)
                      cAAAAGAATGCCAATGCCTGCCTGCTGCTTCATACCGTATTCCCTGCAAAGCGCATTTGCGGATTAACGTCGCACCATGCGCATTTTTACCGGTTATCGAATGTAAAAAGCCCCGCAACGTGTTGCGGGGCTTTCATCCGTTa                                                                                                                       >  2:710896/1‑143 (MQ=255)
                       aaaaGAATGCCAATGCCTGCCTGCTGCTTCATACCGTATTCCCTGCAAAGCGCATTTGCGGATTAACGTCGCACCATGCGCATTTTTACCGGTTATCGAATGTAAAAAGCCCCGCAACGTGTTGCGGGGCTTTCATCCGTTAc                                                                                                                      <  2:754905/143‑1 (MQ=255)
                        aaggAATCCCACTGCCTCACCGCTGCTTCATACCGTCTTCCCTGCAAACCGCATTTGCGGATTAACGTCGCACCATGCGCATTTTTACCGGTTATCGAATGTAAAAAGCCCCGCAACGTGTTGCGGGGCTTTCATCCGTTAcc                                                                                                                     <  2:521517/140‑1 (MQ=255)
                          cgAATGCCAATGCCTTCCTGCTGCTTCATACCGTATTCCCTGCAAAGCGCATTTGCGGATTAACGTCGCACCATGCGCATTTTTACCGGTTATCGAATGTAAAAAGCCCCGCAACGTGTTGCGGGGCTTTCATCCGTTACCgg                                                                                                                   <  1:354581/142‑1 (MQ=255)
                             atgccaatgccTGCCTGCTGCTTCATACCGTATTCCCTGCAAAGCGCATTTGCGGATTAACGTCGCACCATGCGCATTTTTACCGGTTATCGAATGTAACAAGCCCCGCAACGTGTTGCGGGGCTTTCTTCCGTTACCGGGAc                                                                                                                >  1:197617/1‑143 (MQ=255)
                                  agtgcctgccTGCTGCTTCATACCGTATTCCCTGCAAATCGCATTTGCGGATTAACGTCGCGCCATGCGCATTTGTGCCGGTTATCGAATGTAAAAAGCCCCGCAACGTGTTGCGGGGCTTTCATCCGTTACCGGGACGCGaa                                                                                                           <  2:375662/141‑1 (MQ=255)
                                  aatgccTGCCTGCTGCTTCATACCGTATTCCCTGCAAAGCGCATTTGCGGATTAACGTCGCACCATGCGCATTTTTACCGGTTATCGAATGTAAAAAGCCCCGCAACGTCGTGCGGGGCTTTCGTCCGTGACCGCGACGCGaa                                                                                                           >  2:663546/1‑143 (MQ=255)
                                  aaagcctgcctGCTGCTGCATACCGTATCCCCTGCAAAGCGCATTTGCGGATTAACGTCGCACCATGCGCATTTTTACCGGTTATCGAATGTAAAAAGCCCCGCAACGTGTTGCGGGGCTTTCATCCGTTACCGGGACGCGaa                                                                                                           <  1:222353/140‑1 (MQ=255)
                                    tgcctgccTGCTGCTTCATACCGTATTCCCTGCAAAGCGCATTTGTGGATTAACGTCGCACCATGCGCATTTTTACCGGTTATCGAATGTAAAAAGCCCCGCAACGTGTTGCGGGGCTTTCATCCGTTACCGGGACGCGaaaa                                                                                                         <  1:533274/143‑1 (MQ=255)
                                     gcctgcctGCTGCTTCATACCGTATTCCCTGCAAAGCGCATTTGCGGATTAACGTCGCACCATGCGCATTTTTACCGGTTATCGAATGTAAAAAGCCCCGCAACGTGTTGCGGGGCTTTCATCCGTTACCGGGACGCGaaaaa                                                                                                        <  1:499889/143‑1 (MQ=255)
                                      cccgcctgcTTCTTCATACCGGATTCCCTGCAAAGCGCATTTGCGGATTAACGTCGCACCATGCGCATTTTTACCGGTTATCGAATGTAAAAAGCCCCGCAACGTGTTGCGGGGCTTTCATCCGTTACCGGGACGCGAAAAAc                                                                                                       <  2:895793/140‑1 (MQ=255)
                                         gcctgcTGCTTCATACCGTATTCCCTGCAAAGCGCATTTGCGGATTAACGTCGCACCATGCGCATTTTTACCGGTTATCGAATGTAAAAAGCCCCGCAACGTGTTGCGGGGCTTTCATCCGTTACCGGGCAGCGAAAAACTTa                                                                                                    >  1:670805/1‑143 (MQ=255)
                                           ctgctgCTTCATACCGTATTCCCTGCAAAGCGCATTTGCGGATTAACGTCGCACCATGCGCATTTTTACCGGTTATCGAATGTAAAAAGCCCCGCAACGTGTTGCGGGGCTTTCATCCGTTACCGGGACGCGAAAAACTTAtt                                                                                                  <  1:672149/143‑1 (MQ=255)
                                           ctgctgCTTCATACCGTATTCCCTGCAAAGCGCATTTGCGAATTAACGTCGCACCATGCGCATTTTTACCGGTTATCGAATGTAAAAAGCCCCGCACCGTGTTGCGGGGCTTTCATCCGTTACCGGGACGCGAAAAACTTAtt                                                                                                  <  2:730583/143‑1 (MQ=255)
                                                  ttCATACCGTATTCCCTGCAAAGCGCATTTGCGGATTAACGTCGCACCATGCGCATTTTTACCGGTTATCGAATGTAAAAAGCCCCGCAACGTGTTGCGGGGCTTTCATCCGTTACCCGGACGCGAAAAACTTATTCAGcgcc                                                                                           >  1:141686/1‑140 (MQ=255)
                                                     acaCCGTATTCCCTGCAAAGCGCATTGGCGCATTAACGTCGCACCCTGCGCATTTTTACCGGTTATCGAATGGAAAAAGCCCCGCAACGTGTTGCGGGGCTTTCATCCGTTACCGGGACGCGAAAAACTTATTCAGCTTCGAt                                                                                        <  2:115394/141‑1 (MQ=255)
                                                                 cTGCAAAGCGCATTTGCGGATTAACGTCGCACCATGCGCATTTTTACCGGTTATCGAATGTAAAAAGCCCCGCAACGTGTTGCGGGGCTTTCATCCGTTACCGGGACGCGAAAAACTTATTCAGCTTCGATGCTGATAAAttt                                                                            >  1:875825/1‑143 (MQ=255)
                                                                  tGCAAAGCGCATTTGCGGATTAACGTCGCACCATGCGCATTTTTACCGGTTATCGAATGTAAAAAGCCCCGCAACGTGTTGCGGGGCTTTCATCCGTTACCGGGACGCGAAAAACTTATTCAGCTTCGATGCTGATAAATTTa                                                                           >  1:315461/1‑143 (MQ=255)
                                                                   gCAAAGCGCATTTGCGGATTAACGTCGCACCATGCGCATTTTTACCGGTTATCGAATGTAAAAAGCCCCGCAACGTGTTGCGGGGCTTTCATCCGTTACCGGGACGCGAAAAACTTATTCAGCTTCGATGCTGATAAATTTAc                                                                          <  1:241344/143‑1 (MQ=255)
                                                                          gcgtttGCGGATTAACGTCGCACCACGCGCATTTTTACCGGTTATCGAATGTAAAAAGCCCCGCAACGTGTTGCGGGGCTTTCATCCGTTACCGGGACGCGAAAAACTTATTCAGCTTCGATGCTGATAAATTTACGGttttt                                                                   <  1:716635/140‑1 (MQ=255)
                                                                             tttGCGGATTAACGTCGCACCATGCGCATTTTTACCGGTTATCGAATGTAAAAAGCCCCGCAACGTGTTGCGGGGCTTTCATCCGTTACCGGGACGCGAAAAACTTATTCAGCTTCGATGCTGATAAATTTACGGTTTTTCgg                                                                <  2:21512/143‑1 (MQ=255)
                                                                             tttGCGGATTAACGTCGCACCATGCGCATTTTTACCGGTTATCGAATGTAAAAAGCCCCGCAACGTGTTGCGGGGCTTTCATCCGTTACCGGGACGCGAAAAACTTATTCAGCTTCGATGCTGATAAATTTACGGTTTTTCgg                                                                >  2:604907/1‑143 (MQ=255)
                                                                               tGCGGATTAACGTCGCACCATGCGCATTTTTACCGGTTATCGAATGTAAAAAGCCCCGCAACGTGTTGCGGGGCTTTCATCCGTTACCGGGACGCGAAAAACTTATTCAGCTTCGATGCTGATAAATTTACGGTTTTTCGGGc                                                              <  2:795038/143‑1 (MQ=255)
                                                                                  gaaTTAACGTCGCACCATGCGCATTTTTACCGGTTATCGAATGTAAAAAGCCCCGCAACGTGTTGCGGGGCTTTCATCCGTTACCGGGACGCGAAAAACTTATTCAGCTTCGATGCTGATAAATTTACGGTTTTTCGGGCCtt                                                           <  1:502096/141‑1 (MQ=255)
                                                                                   gATTAACGTCGCACCATGCGCATTTTTACCGGTTATCGAATGTAAAAAGCCCCGCAACGTGTTGCGGGGCTTTCATCCGTTACCGGGACGCGAAAAACTTATTCAGCTTCGATGCTGATAAATTTACGGTTTTTCGGGCCttt                                                          >  1:614704/1‑143 (MQ=255)
                                                                                        aCGTCGCACCATGCGCATTTTTACCGGTTATCGAATGTAAAAAGCCCCGCAACGTGTTGCGGGGCTTTCATCCGTTACCGGGACGCGAAAAACTTATTCAGCTTCGATGCTGATAAATTTACGGTTTTTCGGGCCTTTAACtt                                                     >  1:460443/1‑143 (MQ=255)
                                                                                            cGCACCATGCGCATTTTTACCGGTTATCGAATGTAAAAAGCCCCGCAACGTGTTGCGGGGCTTTCATCCGTTACCGGGACGCGAAAAACTTATTCAGCTTCGATGCTGATAAATTTACGGTTTTTCGGGCCTTTAACTTCGaa                                                 <  2:241853/143‑1 (MQ=255)
                                                                                                   tgggcATGTTTACCGGTTATCGAATGTAAAAAGCCCCGCAACGTGTTGCGGGGCTTTCATCCGTTACCGGGACGCGAAAAACTTATTCAGCTTCGATGCTGATAAATTTACGGTTTTTCGGGCCTTTAACTTCGAATTTCACt                                          <  1:364229/140‑1 (MQ=255)
                                                                                                    gcgcATTTTTACCGGTTATCGAATGTAAAAAGCCCCGCAACGTGTTGCGGGGCTTTCATCCGTTACCGGGACGCGAAAAACTTATTCAGCTTCGATGCTGATAAATTTACGGTTTTTCGGGCCTTTAACTTCGAATTTCACtt                                         <  1:429876/143‑1 (MQ=255)
                                                                                                                cGGTGAGCGAATGTAAACAGCCCCGCAACGTGTTGCGGGGCTTTCATCCGTTACCGGGACGCGAAAAACTTATTCAGCTTCGATGCTGATAAATTTACGGTTTTTCGGGCCTTTAACTTCGAATTTCACTTTACCGTCTGCtt                             <  1:232523/143‑1 (MQ=255)
                                                                                                                  gTTATCGAGTGTAAAAAGCCCCGCAACGTGTTGCGGGGCTGTCATCCGTTACCGGGACGCGAAAAACTTATTCAGCTTCGATGCTGATAAATTTACGGTTTTTCGGGCCTTTAACTTCGAATTTCACTTTACCGTCTGCTTTa                           <  1:90666/143‑1 (MQ=255)
                                                                                                                      tCGAATGTAAAAAGCCCCGCAACGTGTTGCGGGGCTTTCATCCGTTACCGGGACGCGAAAAACTTATTCAGCTTCGATGCTGATAAATTTACGGTTTTTCGGGCCTTTAACTTCGAATTTTACTTTACCGTCTGCTTTAGCaa                       >  1:443627/1‑143 (MQ=255)
                                                                                                                                   gCCCCGCAACGTACTACTGTGTTGTCATCCGTTACCGGGACGCGAAATACTTATTTAGCTTCGATGCTTATAAATTTACGGTTTTTCGGGCCTCTAACTTCGAATTTCACTTTACCGTCTGCTTTAGCAAACAGAGTGTGGTc          <  2:817309/143‑1 (MQ=255)
                                                                                                                                    ccccGCAACGTGTTGCGGGGCTTTCATCCGTTACCGGGACGCGAAAAACTTATTCAGCTTCGATGCTGATAAATTTACGGTTTTTCGGGCCTTTAACTTCGAATTTCACTTTACCGTCTGCTTTAGCAAACAGAGTGTGGTCa         >  2:361511/1‑143 (MQ=255)
                                                                                                                                    ccccGCAACACGTTGCGGGGCTTTCATCCGTTACCGGGCCGCGAAAAACTTATTCAGCTTCGATGCTGATAAATTTACGGTTTTTCGGGCCTTTAACTTCGCATTTCACTTTACCGTCTGCTTTAGCAACCAGAGTCTGGGCa         >  1:686704/1‑143 (MQ=255)
                                                                                                                                    ccccGCAACACGTTGCGGGGCTTTCATCCGTTACCGGGACGCGAAAAACTTATTCAGCTTCGATGCTGATAAATTTACGGTTTTTCGGGCCTTTAACTTCGAATTTCACTTTACCGTCTGCTTTAGCAAACAGAGTGTGGTCa         >  1:863327/1‑143 (MQ=255)
                                                                                                                                     cccGCAACGTGTTGCGGGGCTTTCATCCGTTACCGGGACGCGAAAAACTTATTCAGCTTCGATGCTGATAAATTTACGGTTTTTCGGGCCTTTAACTTCGAATTTCACTTTACCGTCTGCTTTAGCAAACAGAGTGTGGTCAc        <  1:469775/143‑1 (MQ=255)
                                                                                                                                      ccGCAACGTGTTGCGGGGCTTTCATCCGTTACCGGGACGCGAAAAACTTATTCAGCTTCGATGCTGATAAATTTACGGTTTTTCGGGCCTTTAACTTCGAATTTCACTTTACCGTCTGCTTTAGCACACAGAGTGCGGTcgcg       >  2:451712/1‑143 (MQ=255)
                                                                                                                                          aaCGTGTTGCGGGGCTTTCATCCGTTACCGGGACGCGAAAAACTTATTCAGCTTCGATGCTGATAAATTTACGGTTTTTCGGGCCTTTAACTTCGAATTTCACTTTACCGTCTGCGTTAGCAAACAGAGTGTGGTCACGACCg   <  1:38634/143‑1 (MQ=255)
                                                                                                                                           aCGTGTTGCGGGGCTTTCATCCGTTACCGGGACGCGAAAAACTTATTCAGCTTCGATGCTGATAAATTTACGGTTTTTCGGGCCCTTAACTTCGATTTTCACTGTACCGTCTGCTTTTGCACACAGCGTGTGGTCACGACCGc  >  2:162780/1‑143 (MQ=255)
                                                                                                                                              |                                                                                                                                           
AGCAAATTGCTGTGGTGAGCGCCAAAAGAATGCCAATGCCTGCCTGCTGCTTCATACCGTATTCCCTGCAAAGCGCATTTGCGGATTAACGTCGCACCATGCGCATTTTTACCGGTTATCGAATGTAAAAAGCCCCGCAACGTGTTGCGGGGCTTTCATCCGTTACCGGGACGCGAAAAACTTATTCAGCTTCGATGCTGATAAATTTACGGTTTTTCGGGCCTTTAACTTCGAATTTCACTTTACCGTCTGCTTTAGCAAACAGAGTGTGGTCACGACCGC  >  NC_000913/3332681‑3332962

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑