| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_000913 | 3,832,188 | A→T | 12.3% | intergenic (+22/+31) | yicH → / ← yicI | AsmA famly protein YicH/alpha‑D‑xyloside xylohydrolase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 3,832,188 | 0 | A | T | 12.3% | 129.5 / 10.0 | 58 | intergenic (+22/+31) | yicH/yicI | AsmA famly protein YicH/alpha‑D‑xyloside xylohydrolase |
| Reads supporting (aligned to +/- strand): ref base A (26/23); new base T (4/3); total (32/26) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.61e-01 | |||||||||||
GAAACCTACGGTTAGCGGGCAATTGCATGCCGTGAACGCCGCAAAGCAGCAAGTGACTCAAACCATGAATGCTGGCATCGTTTCCAGCGGTGAAGTTACATCGACGGAGCCGGTGCGGTAAACATCAATCTCGCCGGGTACGACTCAGACGTACCCGGCATTCCATCAATAGATTACAACGTAATTGTCAGCGCATTCCCTTGAGGCTTCACCACCAGCCCCTGCTCACTTTCAGCCTGCGAACCGTCTTGCAGACCATTTACTTTCACAACATTGCGCAGGC > NC_000913/3832046‑3832328 | gAAACCTGCGGTTAGCGGGAAATTGCACGCCGTGAACGCCGCAAAGCAGCAAGTGACTCAAACCATGAATGCTGGCATCGTTTCCAGCGGTGAAGTTACATCGACGGAGCCGGTGCGGTAAACATCAATCTCGCCGGGTACGa < 2:89295/143‑1 (MQ=255) aCCTACGGTTAGCGGGCAATTGCATGCCGTGAACGCTGCAAAGCAGCAAGTGACTCAAACCATGAATACTGGCATCGTTTCCAGCGGTGAAGGTACATCGACGGAGCCGGCGCGGTCAACAGCAATCTCGACGGGTACGACTc > 2:643733/1‑143 (MQ=255) cTACGGTTAGCGGGCAATTGCATGCCGTGAACGCCGCAAAGCAGCAAGTGACTCAAACCATGAATGCTGGCATCGTTTCCAGCGGTGAAGTTACATCGACGGAGCCGGTGCGGTAAACATCAATCTCGCCGGGTACGACTCAg > 1:221266/1‑143 (MQ=255) aCGGTTAGCGGGCAATTGCATGCCGTGGACGCTGCAAAGCAGCAAGTGACTTAAAACTAAAAACCAATCATCCCGAGCATCGGGGTAGTGACATCGACGGAGCCGGTTCGGTAAACATCAATCTCGCCGGGTCCGACTCacac > 2:188509/1‑143 (MQ=255) gCAATTGCATGCCGTGAACGCCGCAAAGCAGCAAGTGACTCAAACCATGAATGCTGGCATCGTTTCCAGCGGTGAGGTTACATCGACGGAGCCGGTGCGGTAAACATCAATCTCGCCGGGTACGGCTCAGACGTACCCgggct > 2:365017/1‑140 (MQ=255) acttGCATGCCTTGAACGCCGCAAAGCAGCAAGTGACTCAAACCATGAATGCTGGCATCGTTTCCAGCGGTGAAGTTACATCGACGGAGCCGGTGCGGTAAACATCAATCTCGCCGGGTACGTCTGAGTCGTACCCGGcgaga < 2:503609/141‑5 (MQ=255) aaTTGCATGCCGTGAACGCCGCGAAGCAGGAAGGGACTCAAACCATGAATGCGGGCATCGTTTCCAGCGGTGAAGTTACATCGACGGAGCCGGTGCGGTAAACATCAATCTCGCCGGGTACGTCTGAGTCGTACCCGGcgaga < 2:406939/143‑5 (MQ=255) ttGCATGCCGTGAACGCCGCAAAGCAGCAAGTGACTCAAACCATGAATGCTGGCATCGTTTCCAGCGGTGAAGTTACATCGACGGAGCCGGTGCGGTAAACATCAATCTCGCCGGGTACGCCTCCGACGTACCCGGCATCCCa > 1:444684/1‑143 (MQ=255) tGCATGCCGTGAACGCCGCAAAGCAGCAAGTGACTCAAACCATGAATGCTGGCATCGTTTCCAGCGGTGAAGTTACATCGACGGAGCCGGTGCGGTAAACATCAATCTCGCCGGGTACGACTCAGACGTACCCGGCATTCCAt < 1:788519/143‑1 (MQ=255) tGCAGGGCGGGAACGCCGCAAAGCAGCAAGTGACTCAAACCATGAATGCTGGCATCGTTTCCAGCGGTGAAGTTACATCGACGGAGCCGGTGCGGTAAACATCAATCTCGCCGGGTACGACTCAGACGTACCCGGCATTCCAt < 1:406203/143‑1 (MQ=255) cATGCCGTGCACGCCGCAAAGCAGCAAGTGACTCAAACCATGAATGCTGGCATCGTTTCCAGCGGTGAAGTTACATCGACGGAGCCGGTGCGGTAAACATCAATCTCGCCGGGTACGACTCAGACGTACCCGGCATTCCATCa < 1:522190/143‑1 (MQ=255) gCCGCGAACGCGGCACTGCAGTATGTGACTCAAACCATCAATGCTGGCATCGTTTCCAGCGGTGAAGTTACAGCGACGGAGCCGGTGCGGTAAACATCAATCTCGCCGGGTACGACTCAGACGTACCCGGCATTCCATCAATa < 1:159317/143‑1 (MQ=255) aCGCCGCAAAGCAGCAAGTGACTCAAACCATGAATGCTGGCATCGTTTCCAGCGGTGAAGTTACATCGACGGAGCCGGTGCGGTAAACATCAATCTCGCCGGGTACGACTCAGACGTACCCGGCATTCCATCAATAGATTACa > 1:463062/1‑143 (MQ=255) cgcAAAGCAGCAAGTGACTCAAACCATGAATGCTGGCATCGTTTCCAGCGGTGAAGTTACATCGACGGAGCCGGTGCGGTAAACATCAATCTCGCCGGGTACGACTCAGACGTCCCCGGCATTCCATCAATCAGTTACAACGt > 2:221803/1‑143 (MQ=255) cgcAAAGCAGCAAGTGACTCAAACCATGAATGCTGGCATCGTTTCCAGCGGTGAAGTTACATCGACGGAGCCGGTGCGGTAAACATCAATCTCGCCGGGTACGACTCAGACGTACCAGGCAGTCCATCAATAGATTACAACGt > 2:221843/1‑143 (MQ=255) aaGTGACTCAAACCATGAATGCTGGCATCGTTTCCAGCGGTGAAGTTACATCGACGGAGCCGGTGCGGTAAACCTCAATCTCGCCGGGTACGACTCAGACGTACCCGGCATTCCCTCAATAGATTACAACGTAATTGTCAgcg > 2:367692/1‑143 (MQ=255) aaGTGACTCAAACCATGAATGCTGGCATCGTTTCCAGCGGTGAAGTTACATCGACGGAGCCGGTGCGGTAAACATCAATCTCGCCGGGTACGACTCAGACGTACCCGGCATTCCATCAATAGATTACAACGTAATTGTCAgcg > 2:681343/1‑143 (MQ=255) aaGTGACTCAAACCATGAATGCTGGCATCGTTTCCAGCGGTGAAGTTACATCGACGGAGCCGGTGCGGTAAACATCAATCTCGCCGGGTACGACTCAGACGTACCCGGCATTCCATCAATAGATTACAACGTAATTGTCAgcg < 1:414179/143‑1 (MQ=255) tGACTCACACCATGAATGCTGGCATCGTTTCAAGCGCTGAAGGTACATCGACGGAGCCGGTGCGGTAAACATCAATCTCGCCGGGTACGACTCAGACGTACCCGGCATTCCATCAATAGATTACAACGTAATTGTCAGCGCAt < 2:865595/143‑1 (MQ=255) tGACTCAAACCATGAATGCTGGCATCGTTTCCAGCGGTGAAGTTACATCGACGGAGCCGGTGCGGTACACATCAATCTCGCCCGGTACGACTCAGACGTACCCGGCATTCCATCAATCGATTACAACCTACTTGTCACCGCAt > 2:427296/1‑143 (MQ=255) cTCAAACCATGAAGGCTGGCATCGTTTCCAGCGGTGAAGTTACATCGACGGAGCCGGTGCGGTAAACATCAATCTCGCCGGGTACGACTCAGACGTACCCGGCATTCCATCAATAGATTACAACGTAATTGTCAGCGCATTcc < 2:271355/143‑1 (MQ=255) tCAAACCATGAATGCTGGCATCGTTTCCAGCGGTGAAGTTACATCGACGGAGCCGGTGCGGTAAACATCATTCGCGCCGGGTACGACGCAGACGTAGCGGCCATTCCGTCAATAGGTTCCAAGGGAATTGTCAGCGCATTccc > 2:250464/1‑143 (MQ=255) tCAAACCATGAATGCTGGCATCGGTTCCAGCGGTGAAGTTACATCGACGGAGCCGGTGCGGTAAACAGCAATCTCGCCGGGTCGCACTCAAGCGTACGCGACATTCAATCAATAGAATGCAACGACCAAGTCAGCGCAGTccc > 2:300621/1‑143 (MQ=255) aaaCCATGAATGCTGGCATCGTTTCCAGCGGTGAAGTTACATCGACGGAGCCGGTGCGGTAAACATCAATCTCGCCGGGTACGACTCAGACGTACCCGGCATTCCATCAATAGATTACAACGTAATTGTCAGCGCATTCCCtt < 1:24396/143‑1 (MQ=255) aaaCCATCACTGCTGGCCTCGCTTCCAGCGGTGAAGTTACCACCACGTAGCTTTTGCGGTCAACAACCATCTCGCCTGGTACGACTCAGACGTACCCGGCATTCCATCAATAGATTACAACGTAATTGTCAGCGCATTCCCtt < 1:608908/143‑1 (MQ=255) aaCCATGAATGCTGGCATCGTTTCCAGCGGTGAAGTTACATCGACGGAGCCGGTGCGGTAAACATCAATCTCGCCGGGTACGACTCAGACGTACCCGGCATTCCATCAATAGATTACAACGTAATTGTCAGCGCATTCCCTTg > 1:413287/1‑143 (MQ=255) aCCATGAATGCTGGCATCGTTTCCAGCTGTGAAGTTACATCGACGGAGCCGGTGCGGTAAACATCAATCTCGCCGGGTACGACTCAGACGTACCCGGCATTCCATCAATAGATTACAACGTAATTGTCAGCGCATTCCCTTGa < 1:431299/143‑1 (MQ=255) gAATGCTGGCATCGTTTCCAGCGGTGAAGTTACATCGACGGAGCCGGTGCGGTAAACATCAACCTCGCCGGGTACGACTCAGACGTACCCGGCATTCCATCAATAGATTACAACGTAATTGTCAGCGCATTCCCTTGAGGCtt < 1:449251/143‑1 (MQ=255) aTGCTGGCATCGTTTCCAGCGGTGAAGTTACATCGACGGAGCCGGTGCGGTAAACATCAATCTCGCCGGGTACGACTCAGACGTACCCGGCATTCCATCAATAGATTACAACGTAATTGTCAGCGCATTCCCTTGAGGCTTca < 1:428544/143‑1 (MQ=255) tGCTGGCATCGTTTCCAGCGGTGAAGTTACATCGACGGAGCCGGTGCGGTAAACATCAATCTCGCCGGGTACGACTCAGACGTACCCGGCATTCCATCAATAGATTACAACGTAATTGTCAGCGCATTCCCTTGAGGCTTcac < 1:811760/143‑1 (MQ=255) tGCTGGCATCGTTTCCAGCGGTGAAGCTACATCGACGGCGCCGGTGCGGTAAACATCAATCTCGCCGGGTACGTCTCAGACGTACCCGGCATTCCATCAATAGATTACAACGTAATTGTCAGCGCATTCCCTTGAGGCTTcac < 1:86887/143‑1 (MQ=255) cTGGCATCGTTTCCAGCGGTGAAGTTACATCGACGGAGCCGGTGCGGTAAACATCAATCTCGCCGGGTACGACTCAGAc < 1:228284/79‑1 (MQ=255) cTGGCATCGTTTCCAGCGGTGAAGTTACATCGACGGAGCCGGTGCGGTAAACATCAATCTCGCCGGGTACGACTCAGAc > 2:228284/1‑79 (MQ=255) gCATCGTTTCCAGCGGTGAAGTTACATCGACGGAGCCGGTGCGGTAAACATCAATCTCGCCGGGTACGACTCAGACGTACCCGGCATTCCATCAATAGATTACAACGTAATTGTCAGCGCATCCCCTTGAGGCTTCAccacca > 1:453943/1‑143 (MQ=255) ggCGGTGAAGTTACATCGACGGGGCCGGTGCGGTAAACATCAATCTCGCCGGGTACGACTCAGACGTACCCGGCATTCCATCAATAGATTACAACGTAATTGTCAGCGCATTCCCTTGAGGCTTCACCACCAGCCCCTGCTCa < 2:752668/142‑1 (MQ=255) aGCGGTGAAGTTACATCGACGGAGCCGGTGCGGTAAACATCAATCTCGCCGGGTACGACTCAGACGTACCCGGCATTCCATACATCGATTCCAACGTAATTGTCAGCGCATTCCCTCGCGGCTTCACCACCACCCCCTGcccc > 2:279434/1‑140 (MQ=255) agtttgaTCGACGGAGCCGGTGCGGTAAACATCAATCTCGCCGGGTACGACTCAGACGTACCCGGCATTCCATCAATAGATTACAACGTAATTGTCAGCGCATTCCCTTGAGGCTTCACCACCAGCCCCTGCTCACTTTCAGc < 2:585163/137‑1 (MQ=255) tACATCGACGGAGCCGGTGCGGTAAACATCAATCTCGCCGGGTACGACTCAGACGTACCCGGCATTCCATCAATAGATTACAACGTAATTGTCAGCGCATTCCCTTGAGGCTTCACCACCAGCCCCTGCTCACTTTCAGCCTg < 1:607743/143‑1 (MQ=255) acttCGACGGAGCCGGTGCGGGAAACATCATGCTCGCCGGGTACGACTCAGGCGTACCCGGCATTCCATCAATCGATTACAACGTAATTGTCAGCGCATTCCCTTGAGGCTTCACCACCAGCCCCTGCTCACTTTCAGCCTGc < 2:682169/140‑1 (MQ=255) aTCGACGGAGCCGGTGCGGTAAACATCAATCTCGCCGGGTACGACTCAGACGTACCCGGCATTCCATCAATAGATTACAACGTAATTGTCAGCGCATTCCCTTGAGGCTTCACCACCAGCCCCTGCTCACTTTCAGCCTGCGa > 1:514747/1‑143 (MQ=255) tCGACGGAGCCGGTGCGGTAAACATCAATCTCGCCGGGTACGACTCAGACGTACCCGGCATTCCATCAATAGATTACAACGTAATTGTCAGCGCATTCCCTTGAGGCTTCACCACCCGACCCTGCGCACTTTCAGCCTGCGaa > 2:279258/1‑143 (MQ=255) ccGGTGCGGTAAACATCGATGTCTCCGGGTACGTCTCATACGTGCCGTGCAATCCATCCATAGAGTTCAACTGATTTTGCAGCGCATTCCCTTGGTGCTGCACCACCAGCCCCTGCTCACTTTCATCCTGGGCACCGTCTTGc > 2:854687/1‑143 (MQ=255) gcgCGGTAAACATCCATCTCGCCGGGTACGACTCAGACGTACCCGGCATTCCATCAATAGATTACAACGTAATTGTCAGCGCATTCCCTTGAGGTTTCACTACCAGCCCCTGCTCACTTTCAGCCTGCGAACCGTCTTGCAGa < 1:37579/141‑1 (MQ=255) cGGTAAACATCAATCTCGCCGGGTACGACTAAGCCGTACCCGCCCTGCCAACAATAGATTACAACGTAATTGTCAGCGCATGCCCTTGAGGCTTCACCACCCGCGCCTGCTCACTTTCAGCCAGCGCCCCGTCTCGCCGACCa > 1:378266/1‑143 (MQ=255) gTAAACATCAATCTCGCCGGGTACGACTCAGACGTACCCGGCATTCCATCAATAGATTACAACGTAATTGTCAGCGCATTCCCTTGAGGCTTCACCACCAGCCCCTGCACACTTGCGGCCTGCGAACCGTCTTGCGGACCAtt > 1:691859/1‑143 (MQ=255) tCAATCTCGCCGGGTACGACTCAGACGTACCCGGCATTCCATCACTAGATTACAACGTCATTCTCCGCGCACTCCCTTCATGCTTCACCACCACCCCCTGCTCACTTTCAGCCTGCGAACCCTCTTGCAGACCACTCACttgc > 2:173146/1‑141 (MQ=255) aaTCTCGCCGGGTCCGACTCAGACGTACCCGGCATTCCATCAATAGATTACAACGTAATTGTCAGCGCATTCCCTTGAGGCTTCACCACCAGCCCCTGCTCACTTTCAGCCTGCGAACCGTCTTGCAGACCATTTACTTTcac < 1:188509/143‑1 (MQ=255) aaTCTCCTCGGGTACTACCCACACGTACCCGCCATTCCTTCATTAGATTGCATCGCAATTGCAAGCTTTATCTCTTGAGGCGTCACCACCAGCCCCTGTTCACTTTCAGCCTGCGAACCGTCTTGCAGACCATTTACTTTcac < 2:58439/143‑1 (MQ=255) tcGCCGGGTACGACTCAGACGTACCCGGCATTCCATCAATAGATTACAACGTAATTGTCAGCGCATTCCCTTGAGGCTTCACCACCAGCCCCTGCTCACTTTCAGCCTGCGAACCGTCTTGCAGACCATTTACTTTCacaaca > 2:764094/1‑143 (MQ=255) tcGCCGGGGACGACTCAGACGTACCCGGCATTCCATCAATAGATTACAACGTAATTGTCAGCGCATTCCCTTGAGGCTTCACCCCCAGCACCTGCTCACTCCCAGCCTGCGCACCGTCTTGCAAACCATTTACTTTCccacca > 2:645718/1‑143 (MQ=255) gCCGGGTACGACTCAGACGTACCCGGCATTCCATCAATAGATTACAACGTAATTGTCAGCGCATTCCCTTGAGGCTTCACCACCAGCCCCTGCTCACTGTCAGCCTGCGAACCGTCTTGCAGACCATTTACTTTCACAACAtt < 1:783697/143‑1 (MQ=255) cGGGTACGTCTGAGTCGTACCCGGCCTTCCATCAATAGATTACAACGTACTTGTCAGCGCATTCCCTTGAGGCTTCACCACCAGCCCCTGCTCACTTTCAGCCTGCGAACCGTCTTGCAGACCATTTACTTTCACAACAttcc > 2:206253/1‑141 (MQ=255) cGGGTACGTCTGAGTCGTACCCGGCATTCCATCAATAGATTACAACGTAATTGTCAGCGCATTCCCTTGAGGCTTCACCACCAGCCCCTGCTCACTTTCAGCCTGCGAACCGTCTTTCAGACCATTTAATTTCACAACATTgc > 1:423124/1‑143 (MQ=255) cGGGTACGTCTGAGTCGTACCCGGCATTCCATCAATAGATTACAACGTAATTGTCAGCGCATTCCCTTGAGGCTTCACCACCAGCCCCTGCTCACCTGCAGCCTGCGAAGCGACCTCCACACCATTTACTTTCACACCATTgc > 2:669885/1‑143 (MQ=255) gggTACGACTCAGACGTACCCGGCATTCCATCAATAGATTACAACGTACTTGTCACCGCATTCCCTCGAGGCTTCACCACCCGCCCCTGCCCACCTTCAGCCTGCGCACCGCCTAGCCGACCCCTTACTTTCACAGCAtcccc > 2:533984/1‑139 (MQ=255) gggTACGACTCAGACGTACCCGGCATTCCATCAATAGATTACAACGTAATTGTCAGCGCATTCCCTTGAGGCTTCACCACCAGCCCCTGCTCGCTTTCAGCCTGCGAACCGTCTTGCAGACCATTTACTGTCCCAGCATTgcg > 1:392759/1‑143 (MQ=255) tgTACGACGCAGACGTCCCTGACATTCCATGAATAGATTACAACGTAATTGTCAGCGCATTCCCTTGATGCTTCACCACCAGCCCCTGCTCACTTTCAGCCTGCGAACCGTCTTGCAGACCATTTACTTTCACAACATTgcgc < 2:817519/142‑1 (MQ=255) tACGACTCAGACGTACCCGGCATTCCATCAATAGATTACAACGTAATTGTCAGCGCATTCCCTTGAGGCTTCACCACCAGCCCCTGCTCACTTTCAGCCTGCGAACCGTCTTGCAGACCATTTACTTTCACAACATTGCGCAg > 1:784852/1‑143 (MQ=255) cGACTCAGACGTACCCGGCATTCCATCAATAGATTACAACGTAATTGTCAGCGCATTCCCTTGAGGCTTCACCACCAGCCCCTGCTCACTTTCAGCCTGCGCACCGTCTTGCCGACCACTTACTTTCACTACTTTGCGCATGc > 1:273164/1‑143 (MQ=255) | GAAACCTACGGTTAGCGGGCAATTGCATGCCGTGAACGCCGCAAAGCAGCAAGTGACTCAAACCATGAATGCTGGCATCGTTTCCAGCGGTGAAGTTACATCGACGGAGCCGGTGCGGTAAACATCAATCTCGCCGGGTACGACTCAGACGTACCCGGCATTCCATCAATAGATTACAACGTAATTGTCAGCGCATTCCCTTGAGGCTTCACCACCAGCCCCTGCTCACTTTCAGCCTGCGAACCGTCTTGCAGACCATTTACTTTCACAACATTGCGCAGGC > NC_000913/3832046‑3832328 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |