Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,981,102 T→A 11.5% V72V (GTT→GTA)  yifK → putative transporter YifK

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,981,1020TA11.5% 110.4 / 7.4 46V72V (GTT→GTA) yifKputative transporter YifK
Reads supporting (aligned to +/- strand):  ref base T (21/18);  new base A (2/3);  total (23/23)
Fisher's exact test for biased strand distribution p-value = 6.58e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.09e-01

CATTGGCGTCGGCCTGTTTATGGGGGCCGCCAGTACCCTGAAATGGGCCGGGCCATCCGTATTGTTGGCCTATATCATCGCCGGGCTGTTCGTCTTTTTCATCATGCGTTCAATGGGCGAAATGTTGTTCCTCGAACCGGTTACCGGTTCGTTCGCCGTTTATGCGCATCGTTATATGAGCCCGTTCTTTGGCTATCTCACCGCCTGGTCTTACTGGTTTATGTGGATGGCGGTGGGGATCTCTGAAATCACCGCCATTGGCGTTTATGTCCAGTTCTGGTTC  >  NC_000913/3980961‑3981243
                                                                                                                                             |                                                                                                                                             
cATTGGCGTCGGCCTGTTTATGGGGGCCGCCAGTACCCTGAAATGGGCCGGGCCATCCGTATTGTTGGCCTATATCATCGCCGGGCTGTTCGTCTTTTTCATCATGCGTTCAATGGGCGAAATGTTGTTCCTCGAaccggtta                                                                                                                                              >  1:777092/1‑143 (MQ=255)
cATTGGCGTCGGCCTGTTTATGGGGGCCGCCAGTACCCTGAAATGGGCCGGGCCATCCGTATTGTTGGCCTATATCATCGCCGGGCTGTTCGTCTTTTTCATCATGCGTTCAATGGGCGAAATGTTGTTCCTCGAaccggtta                                                                                                                                              >  1:427179/1‑143 (MQ=255)
  ttGGCGTCGGCCTGTTTATGGGGGCCGCCAGTACCCTGAAATGGGCCGGGCCATCCGTATTGTTGGCCTATATCATCGCCGGGCTGTTCGTCTTTTTCATCATGCGTTCAATGGGCGAAATGTTGTTCCTCGGaccgggtacc                                                                                                                                            >  1:644173/1‑143 (MQ=255)
      cGTCGGCCTGTTTATGGGGGCCGCCAGGACCCGGAAATGGGCCGGGCCATCCGTATTGTTGGCGTATATCATCGCCGGGCTGTTCGTCTTTTTCATCCTGCGTTCAATGGGCGAAATGTTGTTCCTCGAaccggttaccggtt                                                                                                                                        <  1:54338/143‑1 (MQ=255)
        tCGGCCTGTTTATGGGGGCCGCCAGTACCCTGAATTGGGCCGGGCCATCCGTATTGTTGGCCTATATCATCGCCGGGCTGTTCGTCTTTTTCATCATGCGTTCAATGGGCGAAATGTTGTTCCTCGAACCGGTAACCGgttcg                                                                                                                                      <  2:681856/143‑1 (MQ=255)
         cGGCCTGTTTATGGGGGCCGCCAGTACCCTGAAATGGGCCGGGCCATCCGTATTGTTGGCCTATATCATCGCCGGGCTGTTCGTCTTTTTCATCATGCGTGCAATGGGCGAAATGTTGTTCCTCGAACCGGTAACCGgttcga                                                                                                                                     <  2:797102/143‑2 (MQ=255)
             cTGTTTATGGGGGCCGCCAGTACCCTGAAATGGGCCGGGCCATCCGTATTGTTGGCCTATATCATCGCCGGGCTGTTCGTCTTTTTCATCATGCGTTCAATGGGCGAAATGTTGTTCCTCGAACCGGTTACCGGTTCGTTCGc                                                                                                                                 >  1:380890/1‑143 (MQ=255)
             cTGTTTATGGGGGCCGCCAGTACCCTGAAATGGGCCGGGCCATCCGTATTGTTGGCCTATATCATCGCCCGGCTGTTCGTCTTTTTCATCATGCCTTCAATGGGCGAAATGTTGTTCCTCGAaccggttgccggttgatgcgc                                                                                                                                 >  2:116103/1‑136 (MQ=255)
             cTGTTTATGGGGGCCGCCAGTACCCTGAAATGGGCCGGGCCATCCGTATTGTTGGCCTATATCATAGCCGGGCTGTTCGTCTTTTTCATCATGCGTTAAATGCGCGAAATGTTGTTCCTCGACCCGGTTAGCGGTTCGTCCGc                                                                                                                                 >  2:702339/1‑143 (MQ=255)
               gTTTATGGGGGCCGCCAGTACCCTGAAATGGGCCGGGCCATCCGTATTGTTGGCCTATATCATCGCCGGGCTGTTCGTCTTTTTCATCATGCGTTCAATGGGCGAAATGTTGTTCCTCGAACCGGTTACCGGTTCGTTCGCCg                                                                                                                               >  2:682197/1‑143 (MQ=255)
                 ttATGGGGGCGGCCAGTACCCTGAAATGGGCCGGGCCATCCGTATTGTTGGCCTATATCATCGCCGGGCTGTTCGTCTTTTTCATCATGCGTTCAATGGGCGAAATGTTGTTCCTCGAACCGGTTACCGGTTCGTTCGCCGtt                                                                                                                             <  1:416434/143‑1 (MQ=255)
                 ttATGGGGGCCGCCAGTACCCTGAAATGGGCCGGGCCATCCGTATTGTTGGCCTATATCATCGCCGGGCTGTTCGTCTTTTTCATCATGCGTTCAATGGGCGAAATGTTGTTCCTCGAACCGGTTACCGGTTCGTTCGCCGtt                                                                                                                             <  2:863984/143‑1 (MQ=255)
                  tATGGGGGCCGCCAGTACCCTGAAATGGGCCGGGCCATCCGTATTGTTGGCCTATATCATCGCCGGGCGGTTCGTCTTTTTCATCATGCGTTCAATGGGCGAAATGTTGTTCCTCGAACCGGTTACCGGTTCGTTCGCCGttt                                                                                                                            <  1:33099/143‑1 (MQ=255)
                  gaTGGGGGCCGCCAGTACCCTGAAATGGGCCGGGCCATCCGTATTGTGGGCCTATATCATCGCCGGGCTGTTCGTCTTTTTCATCATGCGTTCAATGGGCGAAATGTTGTTCCTCGAACCGGTTACCGGTTCGTTCGCCGttt                                                                                                                            <  1:558443/142‑1 (MQ=255)
                    tcgctctcgccAGTACCCTGAAATGGGCCGGGCCATCCGTATTGTTGGCCTATATCATCGCCGGGCTGTTCGTCTTTTTCATCATGCGTTCAATGGGCGAAATGTTGTTCCTCGAACCGGTTACCGGTTCGTTCGCCGTTTAt                                                                                                                          <  1:882324/136‑1 (MQ=255)
                      ggggCCGCCAGTACCCTGAGATGGGCCGGGCCATCCGTATTGTTGGCCTATATCATCGCCGGGCTGTTCGCCTTTTTCATCATGCGTTCAATGGGCGAAATGTTGTTCCTCGAACCGGTTACCGGTTCGTTCGCCGTTTATgc                                                                                                                        <  2:656352/143‑1 (MQ=255)
                          ccgccAGTACCCTGAAATGGGCCGGGCCATCCGTATTGTTGGCCTATATCATCGCCGGGCTGTTCGTCTTTTTCATCATGCGTTCAATGGGCGAAATGTTGTTCCTCGAACCGGTTACCGGTGCGTTCGCCGTTTATGCGCAt                                                                                                                    >  2:332622/1‑143 (MQ=255)
                           cgccAGTACCCTGAAATGGGCCGGGCCATCCGTATTGTTGGCCTATATCATCGCCGGGCTGTTCGTCTTTTTCATCATGCGTTCAATGGGCGAAATGTTGTTCCTCGAACCGGTTACCGGTTCGTTCGCCGTTTATGCGCATc                                                                                                                   >  1:48238/1‑143 (MQ=255)
                            gccAGTACCCTGAAATGGGCCGGGCCATCCGTATTGTTGGCCGATATCATCGCCGGGCTGTTCGTCTTTTTCATCATGCGTTCAATGGGCGAAATGTTGTTCCTCGAACCGGTTACCGGTTCGTTCGCCGTTTATGCGCATCg                                                                                                                  <  2:72547/143‑1 (MQ=255)
                                gTACCCTGAAATGGGCCGGGCCATCCGTATTGTTGGCCTATATCATCGCCGGGCTGTTCGTCTTTTTCATCATGCGTTCAATGGGCGAAATGTTGTTCCTCGAACCGGTTACCGGTTCGTTCGCCGTTTATGCGCATCGTtat                                                                                                              >  1:863821/1‑143 (MQ=255)
                                     cTGAAATGGGCCGGGCCATCCGTATTGTTGGCCTATATCATCGCCGGGCTGTTCGTCGTTTTCCTCATGCGTTCAATGGGCGAAATGTTGTTCCTCGAGCCGGTTACCGGTTCGTTCGACGTTTATGCGCATCGTTATATCAg                                                                                                         >  2:328756/1‑143 (MQ=255)
                                      tGAAATGGGCCGGGCCATCCGTATTGTTGGCCTATATCATCGCCGGGCTGTTCGTCTTTTTCATCATGCGTTCAATGGGCGAAATGTTGTTCCTCGAACCGGTTACCGGTTCGTTCGCCGGTTCTGCGCAGCGTTATATGAGc                                                                                                        >  1:716323/1‑143 (MQ=255)
                                               ccgggccATCCGTATTGTTGGCCTATATCATCGCCGGGCTGTTCGTCTTTTTCATCATGCGTTCAATGGGCGAAATGTTGTTCCTCGGACCGGTTACCGGTTCGTTCGGCGTTTGTGGGCATGGTCAGATGAGCCGGTTCgtt                                                                                               >  2:441554/1‑143 (MQ=255)
                                                cgggccATCCGTATTGTTGGCCTATATCATCGCCGGGCTGTTCGTCTTTTTCATCATGCGTTCAATGGGCGAAATGTTGTTCCTCGAACCGGTTACCGGTTCGTTCGCCGTTTATGCGCATCGTTATATGAGCCCGTTCTTTg                                                                                              <  1:668994/143‑1 (MQ=255)
                                                   gccATCCGTATTGTTGGCCTATATCATCGCCGGGCTGTTCGTCTTTTTCATCATGCGTTCAATGGGCGAAATGTTGTTCCTCGAACCGGTTACCGGTTCGTTCGCCGTTTATGCGCATCGTTATATGAGCCCGTTCTTTGGCt                                                                                           <  1:342232/143‑1 (MQ=255)
                                                     cATCCGTATTGTTGGCCTATCTCATCGCCGGGCTGTTCGTCTTTTTCATCATGCGTTCAATGGGCGACATGTTGTTCCTCGCACCGGTTACCGGTTCGTTCGCCGTTTATGCGCATCGTTATATGACCCCGCTCTTTCGCTAt                                                                                         >  1:255731/1‑143 (MQ=255)
                                                            aTTGTTGGCCTATATCATCGCCGGGCTGTTCGTCTTTTTCATCATGCGTTCAATGGGCGAAATGTTGTTCCTCGAACCGGTTACCGGTTCGTTCGCCGTTTATGCGCATCGTTATATGAGCCCGTTCTTTGGCTATCTCACCg                                                                                  <  1:365954/143‑1 (MQ=255)
                                                               gtttGCCTATATCATCGGCGGGCTGTTCGTCTTTTTCATCATGTGTTCAATGGGCGAAAGGTTGTTCCTTGAACCGGTTACCGGTTCGTTCGCCGTTTATGCGCATCGTTATATGAGCCCGTTCTTTGGCTATCTCACCGCCt                                                                               <  1:750927/143‑1 (MQ=255)
                                                                ttgGCCTATATCATCGCCGGGCTGTTCGTCTTTTGCATCATGTGTTCAATGGGCGAAATGTTGTTCCTCGAACCGGTTACCGGTTCGTTCGCCGTTTATGCGCATCGTTATATGAGCCCGTTCTTTGGCTATCTCACCGCCTg                                                                              >  1:247805/1‑143 (MQ=255)
                                                                  ggCCTATATCATCGCCGGGCTGTTCGTCTTTTTCATCATGCGTTCAATGGGCGAAATGTTGTTCCTCGAACCGGTAACCGgttcgag                                                                                                                                    >  1:673266/1‑85 (MQ=255)
                                                                  ggCCTATATCATCGCCGGGCTGTTCGTCTTTTTCATCATGCGTTCAATGGGCGAAATGTTGTTCCTCGAACCGGTAACCGgttcgag                                                                                                                                    <  2:673266/87‑3 (MQ=255)
                                                                       atatCATCGCCGGGCTGTTCGTCTTTTTCATCATGCGTTGAATGGGCGAAATGTTGTTCAACAAGTATGGCTGCGGTTCGTTCGCCTTTCATCCGCATCCTTATATGAGCCCGTTCTTTGGCTATCTCACCGCCTGGTCTTAc                                                                       <  1:891708/143‑1 (MQ=255)
                                                                            atcGCCGGGCTGTTCGTCTTTTTCATCATGCGTTCAATGGGCGAAATGTTGTTCCTCGAACCGGTTACCGGTTCGTTCGCCGTTTATGCGCATCGTTATATGAGCCCGTTCTTTGGCTATCTCACCGCCTGGTCTTACTGGtt                                                                  >  1:345582/1‑143 (MQ=255)
                                                                            atcGCCGGGCTGCTCGTCTTTTTCATCATGCTTTCAATGGGCGAAATGTTGTTCCTCGAACCGGTTACCGGTTCGTTCGCCGTTTATGCGCATCGTTATATGAGCCCGTTCTTTGGCTATCTCACCGCCTGGTCTTACTGGtt                                                                  <  1:638488/143‑1 (MQ=255)
                                                                                    gCTGTTCGTCTTTTTCATCATGCGTTCAATGGGCGAAATGTTGTTCCTCGAACCGGTTACCGGTTCGTTCGCCGTTTATGCGCATCGCTATATGAGCCCGTTCTTTGGCTATCTCACCGCCCCGTCTTACTGGTGTATGTGGa                                                          >  2:194387/1‑143 (MQ=255)
                                                                                        ttCGTCTTTTTCATCATGCGTTCAATGGGCGAAATGTTGTTCCTCGAACCGGTTACCGGTTCGTTCGCCGTTTATGCGCATCGTTATATGAGCCCGTTCTTTGGCTATCTCACCGCCTGGTCTTACTGGTTCATGTGGATGGc                                                      >  2:274225/1‑143 (MQ=255)
                                                                                           gTCTATTGCATCAGGCGTTCAATGGGCGAAATGTTGTGCCTCGAACCGGGTACCGGTTCGTTCGCCGTTTATGCGCATCGTTATATGAGCCCGTTCTTTGGCTATCGCACCGGCTGGTCTTACTGGTTTATGTGGATGGCGGt                                                   <  1:702339/143‑1 (MQ=255)
                                                                                                     tcatGCGTTCAATGGGCGAAATGTTGTTCCTCGAACCGGTTACCGGTTCGTTCGCCGTTTATGCGCATCGTTATATGAGCCCGTTCTTTGGCTATCTCACCGCCTGGTCTTACTGGTTTATGTGGATGGCGGTGGGGATctct                                         >  2:886218/1‑143 (MQ=255)
                                                                                                      catGCGTTCACTGCTCTAAATGTTGTTCCGCGCCCTGCTTCCCGCTTCTGTCGCCGTTTATGCGCATCGTTATATGAGGCCGTTCTTTGGCTATCTCACCGCCTGGTCTTACTGGTTTATGTGGATGGCGGTGGGGATCTCTg                                        <  2:868137/143‑1 (MQ=255)
                                                                                                       gtGCGTTCAATGGGCGAAATGTTGTTCCTCGAACCGGTTACCGGTTCGTTCGCCGTTTATGCTCATCGTTATATGAGCCCGTTCTTTGGCTATCTCACCGCCTGGTCTTACTGGTTTATGTGGATGGCGGTGGGGATCTCTGa                                       <  1:452703/142‑1 (MQ=255)
                                                                                                                ctGGGCGCACTGTTGTTCGTTGAACCGGGCACCGGTTCTTTCCCCGTTCCTGCGCAGCGTTATCTGAGCCCGTTCTTTGTCTATCTCACCGCCTGGTCTTACTGGTTTATGTGGATGGCGGTGGGGATCTCTGAAATCACCGc                              <  2:662134/142‑1 (MQ=255)
                                                                                                                aTGGGCGAAATGTTGTTCCTCGAACCGGTTACCGGTTCGTTCGCCGTTTATGCGCATCGTTATATGAGCCCGTTCTTTGGCTATCTCACCGCCTGGTCTTACTGGTTTATGTGGATGGCGGTGGGGATCTCTGAAATCACCGc                              >  2:239017/1‑143 (MQ=255)
                                                                                                                       aaaTGTTGTTCCTCGAACCGGTTACCGGTTCGTTCGCCGTTTATGCGCATCGTTATATGAGCCCGTTCTTTGGCTATCTCACCGCCTGGTCTTACTGGTTTATGTGGATGGCGGTGGGGATCTCTGAAATCACCGCCATTGGc                       >  1:555445/1‑143 (MQ=255)
                                                                                                                          tgttgtTCCTCGAACCGGTTACCGGTTCGTTCGCCGTTTATGCGCATCGTTATATGAGCCCGTTCTTTGGCTATCTCACCGCCTGGTCTTACTGGTTTATGTGGATGGCGGTGGGGATCTCTGAAATCACCGCCATTGGCGtt                    <  1:740735/143‑1 (MQ=255)
                                                                                                                           gttgttCCTCGAACCGGTTACCGGTTCGTTCGCCGTTTATGCGCATCGTTAGATGAGCCCGTTCTTTGGCAGTCTCCCAGCCTGGTCTTACGGGTGTGTATGGATGGCGGGGGAGATCTGTGGAATCACCGGCATTGGTGttc                   >  2:719289/1‑142 (MQ=255)
                                                                                                                            ttgttCCTCGAACCGGTTACCGGTTCGTTCGCCGTTTATGCGCATCGTTATATGAGCCCGTTCTTGGGCTATCTCACCGCCTGGTCTTACTGGTTTATGTGGATGGCGGTGGGGATCTCTGAAATCACCGCCATTGGCGTTTa                  >  2:673790/1‑143 (MQ=255)
                                                                                                                            ttgttCCTCGAACCGGTTACCGGTTAGTCAGGCGATTATGCGCATCGTTATGTGTGAGACGACACAACCTATCTAGATTCCTGTTCTTACTGGTTTATGTGCATTGCTGTGGCGATCTCTGCAATCACCGCCATTGGCGGTTa                  >  2:396549/1‑143 (MQ=255)
                                                                                                                               ttCCTCGAACCGGTTACCGGTTCGTTCGCCGTTTATGCGCATCGTTATATGAGCCCGTTCTTTGGCTATCTCACCGCCTGGTCTTACTGGTTTATGTGGATGGCGGTGGGGATCTCTGAAATCACCGCCATTGGCGTTTATGt               <  2:247805/143‑1 (MQ=255)
                                                                                                                               ttCCTCGAACCGGTTACAGGTTCGTTCGCCGTTTATGCGCATCGTTATATGAGCCCGTTATGTGGCTATCTCACCGCCTGGTCTTACTGGGTTATGTGGATGGCGGTGGGGATCCCTGAAATCACTGCCATTGGCGGTTgtgt               >  2:562028/1‑143 (MQ=255)
                                                                                                                                 gaacGAACCGGTAACCGGTTCGTTCGCCGTTTATGCGCATCGTTATATGAGCCCGTTCTTTGGCTATCTGACCGCCTGGTCTTAATGGATTATGTGTATGGCGGTGGGGATCTCAGCAATCACCGCCATTAGCGCTGATGtac             >  2:248807/4‑141 (MQ=255)
                                                                                                                                  cTCGAACCGGTTACCGGTTCGTTCGCCGTTTATGCGCATCGTTATATGAGCCCGTTCTTTGGCTATCTCACCGCCTGGTCTTACTGGTTTATGTGGATGGCGGTGGGGATCTCTGAAATCACCGCCATTGGCGTTTATGTCCa            <  1:637227/143‑1 (MQ=255)
                                                                                                                                        ccggttaccggttCGTTCGCCGTTTATGCGCATCGTTATATGAGCCCGTTCTTTGGCTATCTCACCGCCTGGTCTTACTGGTTTATGTGGATGGCGGTGGGGATCTCTGAAATCACCGCCATTGGCGTTTATGTCCAGTTCTg      <  1:669140/143‑1 (MQ=255)
                                                                                                                                        ccggtaaccggttCGTGCGCCGTCTATGGGCATCGGTATATGAGCCCGTTCTTTGGCTATCTCACCGCCTGGTCTTACTGGTTTATGTGGATGGCGGTGGGGATCTCTGAAATCACCGCCATTGGCGTTTATGTCCAGTTCTg      <  1:349085/143‑1 (MQ=255)
                                                                                                                                        acggttaccggttCGCTCGCCGTTTATGCGCATCGTTATATGAGCCCGTTCTTTGGCTATCTCACCGCCTGGTCTTACTGGTTTATGTGGATGGCGGTGGGGATCTCTGAAATCACCGCCATTGGCGTTTATGTCCAGTTCTg      <  1:283297/142‑1 (MQ=255)
                                                                                                                                         cggttaccggttCGTTCGCCGTTTATGCGCATCGTTATATGAGCTCGTTCTTTGGCTATCTCACCGCCTTGTCTTACTGGTTTATGTGGATGGCGGTGGGGATCTCTGAAATCACCGCCATTGGCGTTTATGTCCAGTTCTgg     <  1:141838/143‑1 (MQ=255)
                                                                                                                                          ggttaccggttCGTTCGCCGTTTATGCGCATCGTTATATGAGCCCGTTCTTTGGCTATCTCACCGCCTGGTCTTACTGGTTTATGTGGATGGCGGTGGGGATCTCTGAAATCACCGCCATTGGCGTTTATGTCCCGTTCTCGt    >  1:547376/1‑143 (MQ=255)
                                                                                                                                            ttaccggttCGTTCGCCGTTGATGCGCATCGTTATATGAGCCCGTTCTTTGGCTATCTCACCGCCTGGTCGTACTGGTTTATGTGGATGGCGGTGGGGATCTCTGAAATCACCGCCATTGGCGTTTATGTCCAGTTCTGGTTc  <  2:86375/143‑1 (MQ=255)
                                                                                                                                             |                                                                                                                                             
CATTGGCGTCGGCCTGTTTATGGGGGCCGCCAGTACCCTGAAATGGGCCGGGCCATCCGTATTGTTGGCCTATATCATCGCCGGGCTGTTCGTCTTTTTCATCATGCGTTCAATGGGCGAAATGTTGTTCCTCGAACCGGTTACCGGTTCGTTCGCCGTTTATGCGCATCGTTATATGAGCCCGTTCTTTGGCTATCTCACCGCCTGGTCTTACTGGTTTATGTGGATGGCGGTGGGGATCTCTGAAATCACCGCCATTGGCGTTTATGTCCAGTTCTGGTTC  >  NC_000913/3980961‑3981243

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑