Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,989,940 C→T 10.4% R276H (CGC→CAC)  hemC ← hydroxymethylbilane synthase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,989,9400CT10.4% 96.9 / 3.1 40R276H (CGC→CAC) hemChydroxymethylbilane synthase
Reads supporting (aligned to +/- strand):  ref base C (19/16);  new base T (2/2);  total (22/18)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.49e-01

AGACGGGCGGGTGACAAGGATACTCATGCCGGGGCGTCTCCGTTATAGACTTCAGCGAGGATCTCGCGCGCGCCGTTATTCAGTAGCTCTTCTGCCAGCGAAATCCCCATTTGTTCGGCATCTTGCGGCGCACCGCGGCGTTCACCGCGAATAATCTGCGAACCGTCCGGCGCGCCGACCAGCGCACGCAGCCAGATTTCGCCATCAATAAGCTCGGCGTAGCTACCAATTGGCACCTGACATCCGCCTTCGAGACGGGTATTCATGGCGCGT  >  NC_000913/3989802‑3990074
                                                                                                                                          |                                                                                                                                      
agaCGGGCGGGTGACAAGGATACTCATGCCGGGGCGTCTCCGTTATAGACTTCAGCGAGGATCTCGCGCGCGCCGTTATTCAGTAGCTCTTCTGCCAGCGAAATCCCCATTTGTTCGGCATCTTGCGGCGCACCGCGGCGTTc                                                                                                                                    >  2:502836/1‑143 (MQ=255)
          gTGACAAGGATACTCATGCCGGGGCGTCTCCGTTATAGACTTCAGCGAGGATCTCGCGCGCGCCGTTATTCAGTAGCTCTTCTGCCAGCGAAATCCCCATTTGTTCGGCATCTTGCGGCGCACCGCGGCGTTCCCCGCGaata                                                                                                                          >  1:893802/1‑143 (MQ=255)
                 ggATACTCATGCCGGGGCGTCTCCGTTATAGACTTCAGCGAGGATCTCGCGCGCGCCGTTATTCAGTAGCTCTTCTGCCAGCGAAATCCCCATTTGTTCGGCATCTTGCGGCGCCCCGCGGCGTTCACCGCGACTAATCTGCg                                                                                                                   >  1:756712/1‑143 (MQ=255)
                   atccTCATGCCGGGGCGTCTCCGTTCTAGACTTCAGCGAGGATCTCGCGCGCGCCGTTATTCAGTAGCTCTTCTGCCAGCGAAATCCCCATTTGTTCGGCATCTTGCGGCGCACCGCGGCGTTCACCGCGAATAATCTGCGaa                                                                                                                 <  2:740175/140‑1 (MQ=255)
                   aTACTCATGCCGGGGCGTCTCCGTTATAGACTTCAGCGAGGATCTCGCGCGCGCCGTTATTCAGTAGCTCTTCTGCCAGCGAAATCCCCATTTGTTCGGCATCTTGCGGCGCCCCGCGGCGTTCACCGCGAATAATCTGCGaa                                                                                                                 >  1:315165/1‑143 (MQ=255)
                       tCATGCCGGGGCGTCTCCGTTATAGACTTCAGCGAGGATCTCGCGCGCGCCGGTATTCAGTAGCACGTCTGCGCGCGAAATCGCCATTTGATCGGCATTTGCCGCCGCACAGGGCCTTTCACAGCGCATAATCTGCGAACCgc                                                                                                             >  2:770584/1‑142 (MQ=255)
                        cATGCCGGGGCGTCTCCGTTATAGACTTCAGCGAGGATCTCGCGCGCGCCGTTATTCAGTAGCTCTTCTGCCAGCGAAATCCCCATTTGTTCGGCATCTTGCGGCGCACCGCGGCGTTCACCGCGAATAATCTGCGAACCGTc                                                                                                            <  2:508635/143‑1 (MQ=255)
                         atgagggggCGTCGCCGTCATAGACTTCAGCGAGGATCTCGCGCGCGCCGTGATTCAGTAGCTCTTCTGCCAGCGAAATCCCCATTTGTTCGGCAGCTTGCGGCGCACCGCGGCGTTCACCGCGAATAATCTGCGAACCGTcc                                                                                                           <  1:140024/138‑1 (MQ=255)
                         aTGCCGGGGCGTCTCCGTTATAGACTTCAGCGAGGATCTCGCGCGCGCCGTTATTCAGTAGCTCTTCTGCCAGCGAAATCCCCATTTGTTCGGCATCTTGCGGCGCACCGCGGCGTTCACCGCGAATAATCTGCGAACCGTcc                                                                                                           >  1:496792/1‑143 (MQ=255)
                          tGCCGGGGCGTCTCCGTTATAGACTTCAGCGAGGATCTCGCGCGCGCCGTTATTCAGTAGCTCTTCTGCCAGCGAAATCCCCATTTGTTCGGCATCTTGCGGCGCACCGCGGCGTTCACCGCGAATAATCTGCGAACCGTCCg                                                                                                          <  1:502836/143‑1 (MQ=255)
                             cGGGGCTTCCCGGTTATAGACTTCAGCGAGGATCCGGCGCGCGCCGTTTGTCAGTAGCTCTTCTGCCAGCGAAATCCCCATTTGTTCGGCATCTTGCGGCGCACCGCGGCGTTCACCGCGAATAATCTGCGAACCGTCCGgcg                                                                                                       <  2:653818/143‑1 (MQ=255)
                                            gtaGACGTCAGCGAGGATCTCGCGCGCGCCCTGCTTCAGTAGCTCTGCTGCCAGCGAAATCCCCATTTGTTCGGCTTCTTGCGGCGCACCGCGGCGTTCACCTCGAATAATCTGCGATCCGTCCGGCGCGCCGACCAGcgcac                                                                                        <  2:240482/142‑1 (MQ=255)
                                            ataGACTTCAGCGAGGATCTCGCGCGCGCCGTTATTCAGTAGCTCTTCTGCCAGCGAAATCCCCATTTGTTCGGCATCTTGCGGCGCACCGCGGTGTTCACCGCGAATAATCTGCGAACCGTCCGGCGCGCCGACCAGcgcac                                                                                        >  2:799350/1‑143 (MQ=255)
                                               gACTTCAGGGAGGCGCGCGTGCGCGCCGTTATTCCGTAGCTCTTCTGCCCGCGATTTGCGCATTTGTTCGGGATCTTGCGGCGCCCCGCGGCGTCCCCCGCGAATGGCCTGCGAACCGTCCGGCGCGCCGACCAGcgcacgca                                                                                     <  2:830422/143‑1 (MQ=255)
                                                  ttCAGCGAGGATCTCGCGCGCGCCGTTATTCAGTAGCTCTTCTGCCAGCGAAATCCCCATTTGTTCGGCCTCTTGCGGCGCACCGCGGCGTTCACCGCGAGTAAGCTGCGAACCGTCCGGCGCGCCGACCAGCGCCCGCAGcc                                                                                  >  1:606595/1‑143 (MQ=255)
                                                  ttCAGCGAGGATCTCGCGCGCGCCGTTATTCAGTAGCTCTTCTGCCAGCGAAATCCCCATTTGTTCGGCATCTTGCGGCGCACCGCGGGGTTCACCGCGAATAATCTGCGAACCGTCCGGCGCGCCGACCAGCGCACGCAGcc                                                                                  >  1:410211/1‑143 (MQ=255)
                                                       cGAGGATCTCGCGCGCGCCGTTATTCAGTAGCTCTTCTGCCAGCGAAATCCCCATTTGTTCGGCATCTTGCGGCGCACCGCGGTGTTCACCGCGAATAATCTGCGAACCGTCCGGCGCGCCGACCAGCGCACGCAGCCAGAtt                                                                             >  2:327205/1‑143 (MQ=255)
                                                        gAGGATCTCGCGCGCGCCGTTATTCAGTAGCTCTTCTGCCAGCGAAATCCCCATTTGTTCGGCATCTTGCGGCGCACCGCGGCGTTCACCGCGAATAATCTGCGAACCGTCCGGCGCGCCGCCCAGCGCACGCAGCCAGAttt                                                                            >  2:256151/1‑143 (MQ=255)
                                                           gATCTCGCGCGCGCCGCTATTCAGTAGCTCTTCTGCCAGCGAAATCCCCATTTGTTCGGCATCTTGCGGCGCACCGCGGCGTTCACCGCGAATAATCTGCGAACCGTCCGGCGCGCCGACCAGCGCACGCAGCCAGATTTCGc                                                                         <  1:578249/143‑1 (MQ=255)
                                                            aTCTCGCGCGCGCCGTTATTCAGTAGCTCTTCTGCCAGCGAAATCCCCATTTGTTCGGCATCTTGCGGCGCACCGCGGCGCTCACCGCGAATAATCTGCGCACCGTCCGGCGCGCCGACCAGCGCCCGCAGCCCGAGTTCGcc                                                                        >  2:811899/1‑143 (MQ=255)
                                                               tcGCGCGCGCCGTTATTCAGTAGCTCTTCTGCCAGCGAAATCCCCATTTGTTCGGCATCTTGCGGCGCACCGCGGCGTTCACCGCGCATAATCTGCGAACCGTCCGCCGCGCCGACCCGCGCACGCAGCCAGAGTTCGCCATc                                                                     >  2:301363/1‑143 (MQ=255)
                                                                     gcgcCGTTATTCAGTAGCTCTTCTGCCAGCGAAACCCCCATTTTTTCTCCATCTTGCGGCGCACCGCGGCGTTCACCGCGAATAATCTGCGAACCGTCCGGCGCGCCGACCAGCGCACGCAGCCAGATTTCGCCATCAATAAg                                                               <  1:884597/143‑1 (MQ=255)
                                                                         cGTTATTCAGTAGCTCTTCTGCCAGCGAAATCCCCATTTGTTCGGCATCTTGCGGCGCACCGCGGCGTTCACCGCGAATAATCTGCGAACCGTCCGGCGCGCCGACCAGCGCACGCAGCCAGATTTCGCCATCAATCAGCCCg                                                           >  2:494708/1‑143 (MQ=255)
                                                                            tATTCAGTAGCTCTTCTGCCAGCGAAATCCCCATTTGTTCGGCATCTTGCGGCGCACCGCGGCGTTCACCGCGAATAATCTGCGAACCGTCCGGCGCGCCGACCAGCGCACGCAGCCAGATTTCGCCATCAATAAGCTCGGCg                                                        >  1:544460/1‑143 (MQ=255)
                                                                               tCAGTAGCTCTTCTGCCAGCGAAACCCCCATTTGTTCGGCATCTTGCGGCGCACCGCGGCGTTCACCGCGAATAATCTGCGAACCGTCCGGCGCGCCGACCAGCGCACGCAGCCAGATTTCGCCATCAATAAGCTCGGCGTAg                                                     <  1:390118/143‑1 (MQ=255)
                                                                                  gTAGCTCTTCTGCCAGCGAAATCCCCATTTGTTCGGCATCTTGCGGCGCACCGCGGCGTTCACCGCGAATAATCTGCGAACCGTCCGGCGCGCCGACCAGCGCACGCAGCCAGATTTCGCCCTCAAGAAGGCCCGCGTAGCTa                                                  >  2:459090/1‑143 (MQ=255)
                                                                                      ctctTCTGCCAGCGAAATCCCCATTTGTTCGGCATCTTGCGGCGCACCGCGGTGTTCACCGCGAATAATCTGCGAACCGTCCGGCGCGCCGACCAGCGCACGCAGCCAGATTTCGCCATCAATAAGCTCGGCGTAGCTACCaa                                              <  1:327205/143‑1 (MQ=255)
                                                                                         ttctGCCAGCGAAATCCCCATTTGTTCGGCATCTTGCGGCGCACCGCGGCGTTCACCGCGAATAATCTGCGAACCGTCCGGCGCGCCGACCAGCGCACGCAGCCAGATTTCGCCATCAATAAGCTCGGCGTAGCTACCAATTg                                           <  1:726107/143‑1 (MQ=255)
                                                                                         ttctGCCAGCGAAATCCCCATTTGTTCGGCATCTTGCGGCGCACCGCGGCGTTCACCGCGAATAATCTGCGAACCGTCCGGCGCGCCGACCAACGCACGCAGCCAGACTTCGCCATCAATAAGCACGGCGTCGCTACCaaata                                           >  2:377372/1‑140 (MQ=255)
                                                                                          tctGCCATCGAAATCCCCATTTGTTCGGCATCTTGCGGCGCACCGCGGCGTTCGCCGCGAATAATCTGCGAACCGTCCGGCGCGCCGACCAGCGCACGCAGCCAGATTTCGCCATCAATAAGCTCGGCGTAGCTACCAATTgg                                          <  2:697788/143‑1 (MQ=255)
                                                                                             gCCAGCGCAAGCCCCATTTGTGCGGCATCTTGCGGCGCACCGCGGCGTTCACCGCGAATAATCTGCGAACCGTCCGGCGCGCCGACCAGCGCACGCAGCCAGATTTCGCCATCAATAAGCTCGGCGTAGCTACCAATTGGCAc                                       <  2:111552/143‑1 (MQ=255)
                                                                                              ccAGCGAAATCCCCATTTGTTCGGCATCTTGCGGCGCACCGCGGCGTTCACCGCGAATAATCTGCGAACCGTCCGGCGCGCCGACCAGCGCACGCAGCCAGATTTCGCCATCAATAAGCTCGGCGTAGCCACCAAGTGGCAcc                                      >  1:552469/1‑143 (MQ=255)
                                                                                                 gCGAAATCCCCATTTGTTCGGCATCTTGCGGCGCACCGCGGCGTTCACCGCGAATAATCTGCGAACCGTCCGGCGCGCCGACCAGCGCACGCAGCCAGATTTCGCCATCAATAAGCTCGGCGTAGCTACCAATTGGCACCTGa                                   <  1:875684/143‑1 (MQ=255)
                                                                                                          ccATTTGTTCGGCATCTTGCGGCGCACCGCGGCGTTCACCGCGAATAATCTGCGAACCGTCCGGCGCGCCGACCAGCGCACGCAGCCAGATTTCGCCATCAATAAGCTCGGCGTAGCTACCAATTGGCACCTGACATCCGCCt                          >  1:681215/1‑143 (MQ=255)
                                                                                                            tttggTTCGGCATCGTGCGGCGCACCGCGGCGTTCACCGCGAATAATCTGCGAACCGTCCGGCGCGCCGACCAGCGCACGCAGCCAGATTTCGCCATCAATAAGCTCGGCGTAGCTACCAATTGGCACCTGACATCCGCCTTc                        <  2:570685/139‑1 (MQ=255)
                                                                                                             tttGTTCGGCATCTTGCGGCGCACCGCGGCGTTCACCGCGCATAATCTGCGAACCGTCCGGCGCGCCGACCAGCGCACGCAGCCAGATTTCGCCATCAATAAGCTCGGCGTAGCTACCAATTGGCACCTGACATCCGCCTTCg                       <  1:746638/143‑1 (MQ=255)
                                                                                                                     gCATCTTGCGGCGCACCGCGGCGTTCACCGCGAATAACCTGCGAACCGTCCGGCGCGCCGAAATGCGCACGACCCCCGATTTCGCCATCCATCACCTCGGCGTAGCTAGCGCTTGCCCCCTCTCCTCCGCCTTCgacgcgcgt               >  1:562608/1‑136 (MQ=255)
                                                                                                                        tCTTGCGGCGCACCGCGGCGTTCACCGCGAATAATCTGCGAACCGTCCGGCGCGCCGACCAGCGCACGCAGCCAGATTTCGCCATCAATAAGCTCGGCGTAGCTACCAATTGGCACCTGACATCCGCCTTCGAGACGAGTact            >  1:548867/1‑141 (MQ=255)
                                                                                                                                cgcACCGCGGTGTTCACCGCGAATAATCTGCGAACCGTCCGGCGCGCCGACCAGCGCACGCAGCCAGATTTCGCCATCAATAAGCTCGGCGTAGCTACCAATTGGCACCTGACATCCGCCTTCGAGACGGGTATTCATGgcgc    <  1:799350/143‑1 (MQ=255)
                                                                                                                                  cACCGCGGCGTTCACCGCGAATAATCTGCGAACCGTCCGGCGCGCCGACCAGCGCACGCAGCCAGATTTCGCCATCAATAAGCTCGGCGTAGCTACCAATTGGCACCTGCCATCCGCCTTCGAGACGCGCCTTCATGGCGCGt  >  1:12489/1‑143 (MQ=255)
                                                                                                                                          |                                                                                                                                      
AGACGGGCGGGTGACAAGGATACTCATGCCGGGGCGTCTCCGTTATAGACTTCAGCGAGGATCTCGCGCGCGCCGTTATTCAGTAGCTCTTCTGCCAGCGAAATCCCCATTTGTTCGGCATCTTGCGGCGCACCGCGGCGTTCACCGCGAATAATCTGCGAACCGTCCGGCGCGCCGACCAGCGCACGCAGCCAGATTTCGCCATCAATAAGCTCGGCGTAGCTACCAATTGGCACCTGACATCCGCCTTCGAGACGGGTATTCATGGCGCGT  >  NC_000913/3989802‑3990074

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑