Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_000913	4,002,903	A→T	5.0%	C97* (TGT→TGA)	yigG ←	inner membrane protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,002,903	0	A	T	5.0%	234.4 / 3.7	80	C97* (TGT→TGA)	yigG	inner membrane protein
Reads supporting (aligned to +/- strand):  ref base A (41/35);  new base T (2/2);  total (43/37)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 2.39e-01

TTCACTTAATGAACCATCGTTCATATATTCCTTACTCATAAATACTCCATAACTATTGTTTTGATGAATCAGTAGGTGCAAGCATTAGCATACTGAAAGTGGAAAAATAACAAATCAAAAAAATCATCGAACCATTGCCTGAACAGGCAAAATCTTCGGCTATCATTGTGATGATAGAGATGATATATACTGCTAATGTACCAAAAACATAAGTTTTTATATAGATGAAACCACTATCACGGAGTCGCTGGCAATTCATGTTGATGACGAGATAATGGA  >  NC_000913/4002761‑4003039                                                                                                                                               |                                                                                                                                         ttCACTTAATGAACCATCGTTCATATATTCCTTACTCATAAATACTCCATAACTATTGTTTTGATGAATCAGTAGGTGCAAGCATTAGCATACTGAAAGTGGAAAAATAACAAATCAAAAAAATCATCGAACCATTGCCTGaa                                                                                                                                          <  1:569124/143‑1 (MQ=255)      ttAATGAACCATCGTTCATATATTCCTTACCCCTAAACACACCAAAACTATTGTTTTGATGAATCAATAAGTCCAACCACAAACACACACAAAAAACAAAAAAAACAAAACAAAAAAAACATCGAACCATTGCCTAAACAAGc                                                                                                                                     >  2:438070/1‑143 (MQ=255)        aaTGAACCATCGTCCATATATTCCTTACTCATAAATACTCCATAACCATTGTTTTGATGAATCAGTAGGTGCAAGCATTAGCATACTGAAAGTGGAAAAATAACAAATCAAAAAAATCATCGAACCATTGCCTGAACAGGCaa                                                                                                                                   <  2:158824/143‑1 (MQ=255)         aggAACCATCGTTCATATATTCCTTACTCATAAATACGCCATAACTATTGTTTTGATGAATCAGTAGGTGCAAGCATTAGCATACTGAAAGTGGAAAAATAACAAATCAAAAAAATCATCGAACCATTGCCTGAACAGGCaaa                                                                                                                                  <  2:41085/141‑1 (MQ=255)         aTGAACCATCGTTCATATATTCCTTACTCATACATACTCCATAACTATTGTTTTGATGAATCAGTAGGTGCAAGCATTAGCATACTGAAAGTGGAAAAATAACAAATCAAAAAAATCATCGAACCATTGCCTGTTCAGGCaat                                                                                                                                  <  1:583985/143‑2 (MQ=255)         aTGAACCATCGTTCATATATTCCTTACTCATAAATACTCCATAACTAGTTTTTTGATGAATCAGGAGGTGCAAGCATTAGCATACTGAAAGTGGAAAAATAACAAATCAAAAAAATCATCGAACCATTGCCTGTTCAGGCaat                                                                                                                                  <  2:9380/143‑2 (MQ=255)                aTCGTTCATATATTCCTTACTCATAAATACTCCATAACTATTGTTTTGATGAATCAGTAGGTGCAAGCATTAGCATACTGAAAGTGGAAAAATAACAAATCAAAAAAATCATCGAACCATTGCCGGAACAGGAAAAATCTTCg                                                                                                                           >  2:709519/1‑143 (MQ=255)                 acGTTCATAGATTCCTTACTCATAAATACTCCATAACTATTGTTTTGATGAATCAGTAGGTGCAAGCATTAGCATACTGAAAGTGGAAAAATAACAAATCAAAAAAATCATCGAACCATTGCCTGAACAGGCAAAATCTTCgg                                                                                                                          <  1:200950/142‑1 (MQ=255)                    ttCATATATTCCTTACTCATAAATACTCCATAACTATTGTTTTGATGAATCAGTAGGTGCAAGCATTAGCATACTGAAAGTGGAAAAATAACAAATCAAAAAAATCATCGAACCATTGCCTGAACAGGCAAAATCTTCGGCTa                                                                                                                       >  2:800995/1‑143 (MQ=255)                    ttCATATATTCCTTACTCATAAATACTCCATAACTATTGTTTTGATGAATCAGTAGGTGCAAGCATTAGCATACTGAAAGTGGAAAAATAACAAATCAAAAAAATCATCGAACCATTGCCTGAACAGGCAAAATCTTCGGCTa                                                                                                                       >  1:385985/1‑143 (MQ=255)                    ttCATATATTCCTTACTCATAAATACTCCATAACTATTGTTTTGATGAATCAGTAGGTGCAAGCATTAGCATACTGAAAGTGGAAAAAGAACAAATCAAAAAAATCATCGAACCATTGCCTGAACAGGCAAAATCTTCGGCTa                                                                                                                       >  1:508402/1‑143 (MQ=255)                     tCATATATTCCTTACTCATAAATACTCCATAACTATTGTTTTGATGAATCAGTAGGTGCAAGCATTAGCATACTGAAAGTGGAAAAATAACAAATCAAAAAAATCATCGAACCATTGCCTGAACAGGCAAAATCTTCGGCTAt                                                                                                                      >  1:361618/1‑143 (MQ=255)                     tCATATATTCCTTACTCATAAATACTCCATAACTATTGTTTTGATGAATCAGTAGGTGCAAGCATTAGCATACTGAAAGTGGAAAAATAACAAATCAAAAAAATCATCGAACCATTGCCTGAACAGGCAAAATCTTCGGCTAt                                                                                                                      <  1:427489/143‑1 (MQ=255)                       atatatTCCTTACTCATAAATACTCCATAACTATTGTTTTGATGAATCAGTAGGTGCAAGCATTAGCATACTGAAAGTGGAAAAATAACAAATCAAAAAAATCATCGAACCATTGCCTGAACAGGCAAAATCTTCGGCTATCa                                                                                                                    >  1:864813/1‑143 (MQ=255)                          tatTCCTTACTCATAAATACTCCATAACTATTGTTTTGATGAATCAGTAGGTGCAAGCATTAGCATACTGAAAGTGGAAAAATAACAAATCAAAAAAATCATCGAACCATTGCCTGAACAAGCAAAATCTTCGGCTATCAtag                                                                                                                 >  2:111133/1‑141 (MQ=255)                            ttCCTTACTCATAAATACTCCATAACTATTGTTTTGATGAATCAGTAGGTGCAAGCATTAGCATACTGAAAGTGGAAAAATAACAAATCAAAAAAATCATCGAACCATTGCCTGAACAGGCAACATCTTAGGCTATCATtgtg                                                                                                               >  2:671353/1‑143 (MQ=255)                             tCCTTACTCAAAAATACTCCATAACAATTGTTTTGATGAATCAGTAGGTGCAAGCATTAGCATACTGAAAGTGGAAAAATAACAAATCAAAAAAATCATCGAACCATTGCCTGAACAGGCAAAATCTTCGGCTATCATTGtga                                                                                                              <  2:808623/143‑1 (MQ=255)                             gccTTACCCATAAATCGTCCATAACTGTTGTTTTGATGAATCAGTAGGTGCAAGCATTAGCATACTGAAAGTGGAAAAATAACAAATCAAAAAAATCATCGAACCATTGCCTGAACAGGCAAAATCTTCGGCTATCATTGtga                                                                                                              <  2:117540/142‑1 (MQ=255)                             aacTTACACAAAAATACTCCATAACTATTGTTTTGAAGAATAAGTAGGTGCAAGCATTAGCATACTGAAAGTGGAAAAATAACAAATCAAAAAAATCATCGAACCATTGCCTGAACAGGCAAAATCTTCGGCTATCATTGtga                                                                                                              <  2:532373/141‑1 (MQ=255)                                 tACTCATAAATACTCCATAACTATTGTTTTGATGAATCAGTAGGTGCAAGCATTAGCATACTGAAAGTGGAAAAATAACAAATCAAAAAAATCATCGAACCATTGCCTGAACAGGCAAAATCTTCGGCTATCATTGTgatgat                                                                                                          <  2:512681/143‑1 (MQ=255)                                    tCATAAATACTCCATAACTATTGTTTTGATGAATCAGTAGGTGCAAGCATTACCATACTGAAAGTGGAAAAATACCAAATCAAAAAAATCATCGAACCATTGCTATAACATGCAAAATCTTCGACTATCATTTTGATGATaga                                                                                                       >  1:266501/1‑143 (MQ=255)                                      aTAAATACTCCATAACTATTGTTTTGATGAATCAGTAGGTGCAAGCATTAGCATACTGAAAGTGGAAAACTAACAAATCAAAAAAATCATCGAACCATTGCCTGAACAGGCAAAATCTTCGGCTATCCTTGTGATGATAgaga                                                                                                     >  1:9331/1‑143 (MQ=255)                                         aaTACTACATCACGATTGTGCTGATGAATCAGTAGTTGCAAGCATTAGCACACGGAAAGTGGAAAAATAACAAATCAAAAAAAGCATCGGACCATTGCCTGAACAGGCAAAATCTTCGGCTATCATTGTGATGATAGAgatga                                                                                                  <  1:162448/143‑1 (MQ=255)                                               ccATAACTATTGTTTTGATGAATCAGTAGGTGCAAGCATTAGCATACTGAAAGTGGAAAAATAACAAATCAAAAAAATCATCGAACCATTGCCTGAACAGGCAAAATCTTCGGCTATCATTGTGATGATAGAGATGAtatata                                                                                            <  1:498851/143‑1 (MQ=255)                                               ccATAACTATTGTTTTGATGAATCAGTAGGTGCAAGCATTAGCATACTGAAAGTGGAAAAATAACAAATCAAAAAAATCATCGAACCATTGCCTGAACAGGCAAAATCTTCGGCTATCATTGTGATGATAGAGATGAtatata                                                                                            >  2:511935/1‑143 (MQ=255)                                                      tATTGTTTTGATGAATCAGTAGGTGCAAGCATTAGCATACTGAAAGTGGAAAAATAACAAATCAAAAAAATCATCGAACCATTGCCTGAACAGGCAAAATCTTCGGCTATCATTGTGATGATAGAGATGATATATACTGCTaa                                                                                     <  1:221648/143‑1 (MQ=255)                                                      tATTGTTTTGATGAATCAGTAGGTGCAAGCATTAGCATACTGAAAGTGGAAAAATAACAAATCAAAAAAATCATCGAACCATTGCCTGAACAGGCAAAATCTTCGGCTATCATTGTGATGATAGAGATGATATATACTGCTaa                                                                                     >  1:656618/1‑143 (MQ=255)                                                      tATTGTTTTGATGAATCAGTAGGTGCAAGCATTAGCATACTGAAAGTGGAAAAATAACAAATCAAAAAAATCATCGAACCATTGCCTGAACAGGCAAAATCTTCGGCTATCATTGTGATGATAGAGATGATATATACTGCTaa                                                                                     >  1:716351/1‑143 (MQ=255)                                                      tATTGTTTTGATGAATCAGTAGGTGCAAGCATTAGCATACTGAAAGTGGAAAAATAACAAATCAAAAAAATCATCGAACCATTGCCTGAACAGGCAAAATCTTCGGCTATCATTGTGATGATAGAGATGATATATACTGATaa                                                                                     >  2:696398/1‑143 (MQ=255)                                                          gTTTTGATGAATCAGTAGGTGCAAGCATTAGCATACTGAAAGTGGAAAAATAACAAATCAAAAAAATCATCGAACCATTGCCTGAACAGGCAAAATCTTCGGCTATCATTGTGATGATAGAGATGATATATACTGCTAATGTa                                                                                 <  1:352401/143‑1 (MQ=255)                                                           ttttGATGAATCAGTAGGTGCAAGCATTAGCATACTGAAAGTGGAAAAATAACAAATCAAAAAAATCATCGAACCATTGCCTGAACAGGCAAAATCTTCGGCTATCATTGTGATGATAGAGATGATATATACTGCTAATGTAc                                                                                >  1:333193/1‑143 (MQ=255)                                                               gatgaATCAGTAGGTGCAAGCATTAGCATACTGAAAGTGGAAAAATAACAAATCAAAAAAATCATCGAACCATTGCCTGAACAGGCAAAATCTTCGGCTATCATTGTGATGATAGAGATGATATATACTGCTAATGTACCaaa                                                                            >  1:580689/1‑143 (MQ=255)                                                                 tgaATCAGTAGGTGCAAGCATTAGCATACTGAAAGTGGAAAAATAACAAATCAAAAAAATCATCGAACCATTGCCTGAACAGGCAAAATCTTCGGCTATCATTGTGATGATAGAGATGATATATACTGCTAATGTACCaaaaa                                                                          <  2:122664/143‑1 (MQ=255)                                                                     tCAGTAGGTGCAAGCATTAGCATACTGAAAGTGGAAAAATAACAAATCAAAAAAATCATCGAACCATTGCCTGAACAGGCAAAATCTTCGGCTATCATTGTGATGATAGAGATGATATATACTGCTAATGTACCAAAAACATa                                                                      >  1:639327/1‑143 (MQ=255)                                                                     tCAGTAGGTGCAAGCATTAGCATACTGAAAGTGGAAAAATAACAAATCAAAAAAATCATCGAACCATTGCCTGAACAGGCAAAATCTTCGGCTATCATTGTGATGATAGAGATGATATATACTGCTAATGTACCAAAAACATa                                                                      >  1:570208/1‑143 (MQ=255)                                                                      cAGTAGGTGCAAGCATTAGCATACTGAAAGTGGAAAAATAACAAATCAAAAAAATCATCGAACCATTGCCTGAACAGGCAAAATCTTCGGCTATCATTGTGATGATGGAGATGATATATACTGCTAATGTACCAAAAACATaa                                                                     <  2:121769/143‑1 (MQ=255)                                                                      cAGTAGGTGCAAGCATTAGCATACTGAAAGTGGAAAAATAACAAATCAAAAAAATCATCGAACCATTGCCTGAACAGGCAAAATCTTCGGCTATCATTGTGATGATAGAGATGATATATACTGCTAATGTACCAAAAACATaa                                                                     <  2:66850/143‑1 (MQ=255)                                                                      cAGTAGGTGCAAGCATTAGCATACTGAAAGTGGAAAAATAACAAATCAAAAAAATCATCGAACCATTGCCTGAACAGGCAAAATCTTCGGCTATCATTGTGATGATAGAGAGGATATGTACTGCTAATGTACCAAAAACATaa                                                                     >  1:45018/1‑143 (MQ=255)                                                                       agaaGGTGCAAGCATTAGCATACTGAAAGTGGAAAAATAACAAATCAAAAAAATCATCGAACCATTGCCTGAACAGGCAAAATCTTCGGCTATCATAGTGATGATAGAGATGATATATAATGCTAAAGTACCAAAAACAAAAg                                                                    >  2:338861/4‑143 (MQ=255)                                                                            gTGCAAGCATTAGCATACTGGAAGTGGAAAAATAAAAAATCAAAAACATCGTCGAAGCATTGCCTGAACAGGCAAAATCTTCGGCTATCATTGTGATGATAGAGATGATATATACTGCTAATGTACCAAAAACATAAGttttt                                                               >  1:489262/1‑143 (MQ=255)                                                                            gTGCAAGCATTAGCATACTGAAAGGGGAAAAATAACAAATCAAAAAAATCATCGAACCATTGCCTGAACAGGCAAAATCTTCGGCTATCATTGTGATGATAGAGATGATATATACTGCTAATGTACCAAAAACATAAGttttt                                                               <  2:119656/143‑1 (MQ=255)                                                                             tGCAAGCATTAGCATACTGAAAGTGGAAAAATAACAAATCAAAAAAATCATCGAACCATTGCCTGAACAGGCAAAATCTTCGGCTATCATTGTGATGATAGAGATGATATATACTGCTAATGTACCAAAAACATAAGTTTTta                                                              >  2:41892/1‑143 (MQ=255)                                                                             tGCAAGCATAAGCATACTGAAAGTGGAAAAATAACAAATCAAAAAAATCATCGAACCATTGCCTGAACAGGCAAAATCTTCGGCTATCATTGTGATGATAGAGATGATATATACTGCTAATGTACCAAAAACATAAGTTTTta                                                              <  2:93331/143‑1 (MQ=255)                                                                              gCAAGCAGTAGCATACTGAAAGTGGAAAAATAACAAATCAAAAAAATCATCGAACCATTGCCTGAACAGGCAAAATCTTCGGCTATCATTGTGATGATAGAGATGATATATACTGCTAATGTACCAAAAACATAAGTTTTtat                                                             <  2:571224/143‑1 (MQ=255)                                                                               cAAGCATTAGCATACTGAAAGTGGAAAAATAACGATTCAAAAAAATCATCGAACCATTGCCTGAACAGGCAAAATCTTCGGCTATCATTGTGATGATAGAGATGATATATACTGCTAATGTACCAAAAACATAAGTTTTtata                                                            <  2:183178/143‑1 (MQ=255)                                                                               cAAGCATTAGCATACTGAAAGTGGAAAAATAACAAATCAAAAAAATCATCGAACCATTGACTGAACAGGCAAAATCAACGGCTATCATTGTGATGATAGAGATGATATATACTGCTAATGTACCAAAAACATAAGTTTGtatc                                                            >  2:382512/1‑142 (MQ=255)                                                                                aaGCATTAGCATACTGAAAGTGGAAAAATAACAAATCAAAAAAATCATCGAACCATTGCCTGAACAGGCAAAATCTTCGGCTATCATTGTGATGATAGAGGTGATATATACTGCTAATGTACCAAAAACATAAGTTTTtatat                                                           >  1:85610/1‑143 (MQ=255)                                                                                  gCATTAGCATACTGAAAGTGGAAAAATAACAAATCAAAAAAATCATCGAACCATTGCCTGAACAGGCAAAATCTTCGGCTATCATTGTGATGATAGAGATGATATATACTGCTAATGTACCAAAAACATAAGTTTTTATATAg                                                         >  2:626182/1‑143 (MQ=255)                                                                                           tACTGAAAGTGGAAAAATAACAAATCAAAAAAATCATCGAACCATTGCCTGAACAGGCAAAATCTTCGGCTATCATTGTGATGATAGAGATGATATATACTGCTAATGTACCAAAAACATAAGTTTTTATATAGATGAAACCa                                                >  2:455714/1‑143 (MQ=255)                                                                                            aCTGAAAGTGGAAAAATAACAAATCAAAAAAATCATCGAACCATTGCCTGAACAGGCAAAATCTTCGGCTATCATTGTGATGATAGAGATGATATATACTGCTAATGTACCAAAAACATAAGTTTTTATATAGATGAAACCAc                                               >  1:501382/1‑143 (MQ=255)                                                                                            aCTGAAAGTGGAAAAATAACAAATCAAAAAAATCATCGAACCATTGCCTGAACAGGCAAAATCTTCGGCTATCATTGTGATGATAGAGATGATATAAACTGCTAATGTACCAAAAACAAAAGTTTTTATCTAGATGAAACCAc                                               >  2:584630/1‑143 (MQ=255)                                                                                             cTGAAAGTGGAAAAATAACAAATCAAAAAAATTATCGAACCATTGCCTGAACAGGCAAAATCTTCGGCTATCATTGTGATGATAGAGATGATATATACTGCTAATGTACCAAAAACATAAGGCTTTATATAGATGAAACCACt                                              >  2:469192/1‑143 (MQ=255)                                                                                              tGAAAGTGGAAAAATAACAAATCAAAAAAATCATCGAACCATTGCCTGAACAGGCAAAATCTTCGGCTATCATTGTGATGATAGAGATGATATATACTGCTAATGTACCAACAACATAAGCTTTTATATAGATGAAACCACTa                                             >  2:295954/1‑143 (MQ=255)                                                                                              tGAAAGTGGAAAAATAACAAATCAAAAAAATCATCGAACCATTGCCTGAACAGGCAAAATCTTCGGCTATCATTGTGATGATAGAGATGATATATACTGCTAATGTACCAAAAACATAAGTTTTTATATAGATGAAACCACTa                                             >  1:129639/1‑143 (MQ=255)                                                                                              tGAAAGTGGAAAAATAACAAATCAAAAAAATCATCGAACCATTGCCTGAACAGGCAAAATCTTCGGCTAGCATTATGATGATAGCGATGATAAATACTGCTAATGTGCCAAAAACAGAAGCTTTTATATAGATGAAACCACTa                                             >  1:777473/1‑143 (MQ=255)                                                                                                aaaGTGGAAAAATAACAAATCAAAAAAATCATCGAACCATTGCCTGAACAGGCAAAATCTTCGGCTATCATTGTGATGATAGAGATGATATATACTGCTAATGTACCAAAAACATAAGTTTTTATATAGATGAAACCACTATc                                           >  1:845711/1‑143 (MQ=255)                                                                                                 aaGGGGAAAAATAACAAAACAAAAAAATCATCGAACCATTGCCTGAACAGGCAAAATCTTCGGCTATCATTGTGATGATAGAGATGATATATACTGCTAATGTACCAAAAACATAAGTTTTTATATAGATGAAACCACTATCa                                          <  2:145042/143‑1 (MQ=255)                                                                                                    tGGAAAAATAACAAATCAAAAAAATCATCGAACCATTGCCTGAACCGGCAAAATCTTCGGCTATCATTGTGATGATAGAGATGATATATACTGCTAATGTACCAAAAACCTCAGTTTTTATATAGATGAAACAACTATCACgg                                       >  2:500585/1‑143 (MQ=255)                                                                                                     ggAAAAATAACAAATCAAAAAACTCATCGAACCATTGCCTGAACAGGCAAAATCTTCGGCTATCATTGTGATGATAGAGATGATATATACTGCTAATGTATCAAATACATAAGTTTTTATATAGATGAAACCACTATCACGGa                                      <  2:874818/143‑1 (MQ=255)                                                                                                     ggAAAAATAACAAATCAAAAAAATCATCGAACCATTGCCTGAACAGGCAAAATCTTCGGCTATCATTGTGATGATAGAGATGATATATACTGCTAATGTACCAAAAACATAAGTTTTTATATAGATGAAACCACTATCACGGa                                      <  2:154558/143‑1 (MQ=255)                                                                                                     ggAAAAATAACAAAACAAAAAAATCATCGAACCATTGCCTGAACAGGCAAAATCTTCGGCTATCATTGTGATGATAGAGATGATATATACTGCTAATGTACCAAAAACATAAGTTTTTATATAGATGAAACCACTATCACGGa                                      <  2:150116/143‑1 (MQ=255)                                                                                                      gAAAAATAACAAATCAAAAAAATCATCGAACCATTGCCTGAACAGGCAAAATCTTCGGCTATCATTGTGATGATAGAGATGATATATACTGCTAATGTACCAAAAACATAAGTTTTTATATAGATGAAAACACTATCACGGAg                                     >  2:810168/1‑143 (MQ=255)                                                                                                           aTAACAAATCAAAAAAAGCAGCTAACCATTGCCTGAAAAGGCAAAATCTTCGGCTATCATTGTAATGATAGAGATGATATATACTGCTAATGTACCAAAAACATAAGTTTTTATATAGATGAAACCACTATCACGGAGTCGCt                                <  2:333193/143‑1 (MQ=255)                                                                                                            tAAAAAATCAAAAAAATCATCGAACCATTGCCTGAACAGGCAAAATCTTCGGCTATCATTGTGATGATAGAGATGATATATACTGCTAATGTACCAAAAACATAAGTTTTTATATAGATGAAACCACTATCACGGAGTCGCTg                               <  1:16636/143‑1 (MQ=255)                                                                                                             aaCAAATCAAAAAAATCATCGAACCATTGCCTGAACAGGCAAAATCTTCGGCTATCATTGTGATGATAGAGATGATATATACTGCTAATGTACCAAAAACATAAGTTTTTATATAGATGAAACCACTATCACGGAGTCGCTgg                              >  2:406611/1‑143 (MQ=255)                                                                                                               cAAATCAAAAAAATGATCGAACCATTGCCTGAACAGGCAAAATCGTCGGCTATCATTGTGATGATAGAGATGATATATACTGCTAATGTACCAAAAACATAAGTTTTTATATAGATGAAACCACTATCACGGAGTCGCTGGCa                            <  2:550599/143‑1 (MQ=255)                                                                                                                aaaTCAAAAAAATCATCGAACCATTGCCTGAACAGGCAAAATCTTCGGCTATCATTGTGATGATAGAGATGATATATACTGCTAATGTACCAAAAACATAAGTTTTTATATAGATGAAACCACTATCACGGAGTCGCTGGCaa                           >  1:719415/1‑143 (MQ=255)                                                                                                                   tCAAAAAAATCATCGAACCATTGCCTGAACAGGCAAAATCTTCGGCTATCATTGTGATGATAGAGATGATATATACTGCTAATGTACCAAAAACATAAGTTTTTATATAGATGAAACCACTATCACGGAGTCGCTGGCAATTc                        <  2:656618/143‑1 (MQ=255)                                                                                                                   tCAAAAAAATCATCGAACCATTGCCTGAACAGGCAAAATCTTCGGCTATCATTGTGATGATAGAGATGATATATACTGCTAATGTACCAAAAACATAAGTTTTTATATAGATGAAACCACTATCACGGAGTCGCTGGCAATTc                        >  1:377018/1‑143 (MQ=255)                                                                                                                     ataggaaTCATCGAACCATTGCCTGAACAGGCAAAATATTCGGCTATCATGGTGATGATAGAGATGATATATACTGCTAATGTACCAAAAACATAAGTTTTTATATAGATGAAACCACTATCACGGAGACGCTGGCAATTCAt                      >  2:113280/6‑143 (MQ=255)                                                                                                                             catcGAACCATTGCCTGAACAGGCAAAATCTTCGGCTATCATTGTGATGATAAAGATGATATATACTGCTAATGTACCAAAAACATAAGTTTTTATATAGATGAAACCACTATCACGGAGTCGCTGGCAATTCATGTtgatga              <  2:9331/143‑1 (MQ=255)                                                                                                                              atcGAACCATTGCCTGAACAGGCAAAATCTTCGGCTATCATTGTGATGATAGAGATGATATATACTGCTAATGTACCAAAAACATAAGTTTTTATATAGATGAAACCACTATCACGGAGTCGCTGGCAATTCATGTTGATGAc             <  2:809872/143‑1 (MQ=255)                                                                                                                                cGAACCATTGCCTGAACAGGCAAAATCTTCGGCTATCATTGTGATGATAGAGATGATATATACTGCTAATGTACCAAAATCATAAGTTTTTATATAGATGAAACCACTATCACGGAGTCGCTGGCAATTCATGTTGATGACga           <  2:266501/143‑1 (MQ=255)                                                                                                                                cGAACCATTGCCTGAACAGGCAAAATCTTCGGCTATCATTGTGATGATAGAGATGATATATACTGCTAATGTACCAAAAACATAAGTTTTTATATAGATGAAACCACTATCACGGAGTCGCTGGCAATTCATGTTGATGACga           <  1:455714/143‑1 (MQ=255)                                                                                                                                 aagattttGCCTGTTCAGGCAAAATCTTCGGCTATCATTGTGATGATAGAGATGATATATACTGCTAATGTACCAAAAACATAAGTTTTTATATAGATGAAACCACTATCACGGAGGCGCTGGCAATTCATGTTGATGACgag          >  2:428032/7‑143 (MQ=255)                                                                                                                                  aaCCATTGCCTGAACAGGCAAAATCTTCGGCTATCATTGTGATGATAGAGATGATATATACTGCTAATGTACCAAAAACATAAGTTTTTATATAGATGAAACCACTATCACGGAGTCGCTGGCAATTCATGTTGATGACgaga         <  2:278411/143‑1 (MQ=255)                                                                                                                                     ttttGCCTGTTCAGGCAAAATCTTCGGCTATCATTGTGCTGCTAGGGATGCTATTTACTGCTCATGTACCAACAACACAAGTTCTTATATAGATGAAACCACTACCCCGGAGTCGCTCGCAATCCATGTTGATGACGAGACaa      >  1:360288/3‑143 (MQ=255)                                                                                                                                      aTTGCCTGAACAGGCAAAATCTTCGGCTATCATTGTGATGATAGAGATGATATATACTGCTAGTGTACCAAAAACATAAGTTCTTATATAGATGAGACCACTATCACGGAGGCGCTGGCAATTCATGTTGATGACGAGATAAt     >  1:122687/1‑143 (MQ=255)                                                                                                                                      aTTGCCTGAACAGGCAAAATCTTCGGCTATCATTGTGATGATAGAGATGATATATACTGCTAATGTACCAAAAACATAAGTTTTTATATAGATGAAACCACTATCACGGAGTCGCTGGCAATTCATGTTGATGACGAGATAAt     <  2:580689/143‑1 (MQ=255)                                                                                                                                         gCCTCAACAGGCAAAATCTTCGGCTATCATTGTGATGATAGAGATGATATATACTGCTAATGTACCAAAAACATAAGTTTTTATATAGATGAAACCACTATCACGGAGTCGCTGGCAATTCATGTTGATGACGAGATAATGGa  <  2:364974/143‑1 (MQ=255)                                                                                                                                         cacTGAACACGGAAACTCTTCGGCTATCATTGTGATGATACAGATGATATATACTGCTAATGTACCAAAAACATAAGTTTTTATATAGATGAAACCACTATCACGGAGTCGCTGGCAATTCATGTTGATGACGAGATAATGGa  <  1:769466/141‑1 (MQ=255)                                                                                                                                               |                                                                                                                                         TTCACTTAATGAACCATCGTTCATATATTCCTTACTCATAAATACTCCATAACTATTGTTTTGATGAATCAGTAGGTGCAAGCATTAGCATACTGAAAGTGGAAAAATAACAAATCAAAAAAATCATCGAACCATTGCCTGAACAGGCAAAATCTTCGGCTATCATTGTGATGATAGAGATGATATATACTGCTAATGTACCAAAAACATAAGTTTTTATATAGATGAAACCACTATCACGGAGTCGCTGGCAATTCATGTTGATGACGAGATAATGGA  >  NC_000913/4002761‑4003039

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑