| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_000913 | 4,028,910 | G→C | 11.1% | Q688E (CAG→GAG) | fadB ← | dodecenoyl‑CoA delta‑isomerase, enoyl‑CoA hydratase, 3‑hydroxybutyryl‑CoA epimerase, 3‑hydroxyacyl‑CoA dehydrogenase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 4,028,910 | 0 | G | C | 11.1% | 93.4 / 5.9 | 37 | Q688E (CAG→GAG) | fadB | dodecenoyl‑CoA delta‑isomerase, enoyl‑CoA hydratase, 3‑hydroxybutyryl‑CoA epimerase, 3‑hydroxyacyl‑CoA dehydrogenase |
| Reads supporting (aligned to +/- strand): ref base G (20/12); new base C (2/2); total (23/14) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 6.34e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.99e-01 | |||||||||||
CATTGTGACTCCTTAAGCCGTTTTCAGGTCGCCAACCGGACGGGCTGGCTCAACCGGAGGATAGTACGGTTCGTTATGACGCGCTTTATTACGCAGACCTTCCGGCACTTCATACAGCGGGCCGAGGTGCTGATATTGCTGTGCCATATCGAGGTATTTTGCGCTACCGAGGGTGTCCAGCCAGCGGAACGCGCCGCCGTGGAACGGAGGGAAGCCCAGGCCGTAGACCAGCGCCATATCCGCTTCCGCCGGAGTGGCGATAATGCCTTCCTCCAGACAGCG > NC_000913/4028770‑4029051 | cATTGTGACTCCTTAAGCCGTTTTCAGGTCGCCCACCGGCCGGGCTGGCTAAACCAGCGGAGAGTACGGTTCGGTATGGCGCGCCTTATTACGCCGAGCGTTCGGCACTTCGGACAGCGGGCGGGGGTGCTGATATTGCtgtg > 1:165648/1‑143 (MQ=255) ccTTCAGCCGGTTTCAGGTGTCCAACCGGACGGGCTGGCTCAACCGGAGGATAGTACGGTTCGTTATGACGCGCTTTATTACGCAGACCTTCCGGCACTTCATACAGCGGGCCGAGGTGCTGATATTGCTGTGCCATATCGAg < 2:868681/143‑1 (MQ=255) ccGTTCTCAGTTCGCCACCCGGACGGGCTGGCTCAACCGGACCAGAGTCCGTTTCGTTATGACGCGCTTTATTACGCAGTCCTCCCGGCACTCCATACCGCGGGCCGTGGTGCTGATATTGCTGTGCCATATCGAGGTAtttt < 2:143146/143‑1 (MQ=255) cGTTTTCAGGTCGCCAACCGGACGGGCTGGCTCAACCGGAGGATAGTACGGTTCGTTATGGCGCGGGGTATTGCGCAGACCTTCCGGCACTTCATACAGCGGGCCGAGGTGCTGATATTGCTGTGCCATATCGAGGGAttttt > 1:623945/1‑142 (MQ=255) ggTCGCCAACCGGACGGGCTGGCTCAACCGGAGGATAGTACGGTTCGTTATGACGCGCTTTATTACGCAGACCTTCCGGCACTTCATACAGCGGGCCGAGGTGCTGATATTGCTTTGCCCTCTCGAGCTCTTTTGCCCTcccc > 1:598135/1‑142 (MQ=255) gTCGCCAACCGGACGGGCTGGCTCAACCGGAGGATAGTACGGTTCGTTATGACGCGCTTTATTACGCAGACCTTCCGGCACTTCATACAGCGGGCCGAGGGGCTGATATTGCTGTGCCATCTCGAGGTACTTTGGGCTACCgt > 2:836519/1‑142 (MQ=255) tccccgaCCGGACGTGCTGGCTCAACCGGAGGATAGTACGGTTCGTTATGACGCGCTTTATTACGCAGACCTTCCGGCACTTCATACAGCGGGCCGAGGTGCTGATATTGCTGTGCCATATCGAGGTATTTTGCGCTACCGAg < 2:212832/137‑1 (MQ=255) tCGCCAACCGGACGGGCTGGCTCAACCGGAGGATAGTACGGTTCGTTATGACGCGCTTTATTACGCAGACCTTCCGGCACTTCATACAGCGGGCCGAGGTGCTGATATTGCTGTGCCATATCGAGGTATTTTGCGCTACCGAg > 1:102016/1‑143 (MQ=255) ggACGGGCTGACTCAACCGGAGGATAGTACGGTTCGTTATGACGCGCTTTATTACGCAGACCTTCCGGCACTTCATACAGCGGGCCGAGGTGCTGATATTGCTGTGCCATATCGAGGTATTTTGCGCTACCGAGGGTGTccag < 1:540489/143‑1 (MQ=255) tCAACCGGAGGATAGTACGGTTCGTTATGACGCGCTTTATTACGCAGACCTTCCGGCACTTCATACAGCGGGCCGAGGTGCTGATATTGCTGTGCCATATCGAGGAAGTTTGCGCTGCCGAGGGTGTCCAGCCAGCGGAgcgc > 1:452262/1‑143 (MQ=255) cAACCGGAGGATAGTACGGTTCGTTATGACGCGCTTTATTACGCAGACCTTCCGGCACTTCATACAGCGGGCCGAGGTGCTGATATTGCTGTGCCATATCGAGGTATTTTGCGCTACCGAGGGTGTCCAGCCAGCGGAAcgcg < 2:551062/143‑1 (MQ=255) gaggaTAGTACGGTTCGTTATGACGCGCTTTATTACGCAGACCTTCCGGCACTTCATACAGCGGGCCGAGGTGCTGATATTGCTGTGCCATATCGAGGCATTTTGCGCTCCCGAGGGTGTGCAGCCACCGCAGCGCGccgccg > 2:124977/1‑143 (MQ=255) tACGGTTCGTTATGACGCGCTTTATTACGCAGACCTTCCGGCACTTCATACAGCGGGCCGAGGTGCTGATATTGCTGTGCCATATCGAGGTATTTTGCGCTACCGAGGGTGTCCAGCCAGCGGAACGCGCCGCCGTGGACCgg > 2:256776/1‑143 (MQ=255) tACGGTTCGTTATGACGCGCTTTATTACGCAGACCTTCCGGCACTTCATACAGCGGGCCGAGGTGCTGATATTGCTCTGCCATATCGAGGTATTTTGCGCTACCGAGGGTGTCCAGCCAGCGGAACGCGCCGCCGTGGAACgg > 1:812857/1‑143 (MQ=255) gaCGGTTCGTTATGACGCGCTTTATTACGCAGACCTTCCGGCACTTCATACAGCGGGCCGAGGTGCTGATATTGCTCTGCCATATCGAGGTATTTTGCGCTACCGAGGGTGTCCAGCCAGCGGAACGCGCCGCCGTGGAACgg > 1:805600/2‑143 (MQ=255) aCGGTTCGTTATGACGCGCTTTATTACGCAGACCTTCCGGCACTTCATACAGCGGGCCGAGGTGCTGATATTGCTGTGCCATATCGAGGTATTTTGCGCTACCGAgg < 2:661987/107‑1 (MQ=255) aCGGTTCGTTATGACGCGCTTTATTACGCAGACCTTCCGGCACTTCATACAGCGGGCCGAGGTGCTGATATTGCTGTGCCATATCGAGGTATTTTGCGCTACCGAgg > 1:661987/1‑107 (MQ=255) ggTTCGTTATGACGCGCTTTATTACGCAGACCTTCCGGCACTTCATACAGCGGGCCGAGGTGCTGATATTGCTGTGCCATATGGAAGTATTTTGCGCTACCGACGAGGGGCAGCCCGCGGAACGCGACGCCGGGGAACGGAgg > 2:892788/1‑143 (MQ=255) ggTTCGTTATGACGCGCTTTATTACGCAGACCTTCCGGCACTTCATACAGCGGGCCGAGGTGCTGATATTGCTGTGCCATATCGAGGTATTTTGCGCTACCGAGGGTGTCCAGCCAGCGGAACGCGCCGCCGTGGAACGGacg > 1:466622/1‑141 (MQ=255) gTTCGTTATGACGCGCTTTATTACGCAGACCTTCCGGCACTTCATACAGCGGGCCGAGGTGCTGATATTGCTCTGCCATATCGAGGTATTTTGCGCTACCGAGGGTGTCCAGCCAGCGGAACGCGCCGCCGTGGAACGGAggg < 2:812857/143‑1 (MQ=255) gTTCGTTATGACGCGCTTTATTACGCAGACCTTCCGGCACTTCATACAGCGGGCCGAGGTGCTGATATTGCTCTGCCATATCGAGGTATTTTGCGCTACCGAGGGTGTCCAGCCAGCGGAACGCGCCGCCGTGGAACGGAggg < 2:805600/143‑1 (MQ=255) tCGTGATGACGCGGTTGATGACGCGGGCCTTCCGGCACTTCATACAGCGGGCCGAGGTGCTGATATTGCTGTGCCATATCGAGGTATTTTGCGCTACCGAGGGTGTCCAGCCAGCGGAACGCGCCGCCGTGGAACGGAGGGaa < 2:461699/143‑1 (MQ=255) ccGTTATGGCGCGCTTTATGACGCAGACCTTCCGGCACTTCATACAGCGGGCCGAGGTGCTGATATTGCTGTGCCATATCGAGGTATTTTGCGCTACCGAGGGTGTCCAGCCAGCGGAACGCGCCGCCGTGGAACGGAGGGaa < 2:828639/142‑1 (MQ=255) aCGCGCTTTATTACGCAGACCTTCCGGCACTTCATACAGCGGGCCTAGGTGCTGATATTGCTGTGCCATATCGAGGTATTTTGCGCTACCGAGGGTGTCCAGCCAGCGGAACGCGCCGCCGTGGAACGGAGGGAAGCCCAGGc < 1:502868/143‑1 (MQ=255) gcgcTTTATTACGCAGACCTTCCGGCACTTCATACAGCGGGCCGAGGTGCTGATATTGCTGTGCCATATCGAGGTATTTTGCGCTACCGAGGGTGTCCCGCCCGCGGAACGCGCCGCCGTGGAACGGAGGGAAGCCCAGGCCg > 1:36000/1‑143 (MQ=255) ccGCAGCCCTCCCGGCACTTCGTACAGCGGGCCGAGGTGCCGAGATTGCTGGGCCATATCGGGGTATTTTGCGCTACCGAGGGTGTCCAGCCAGCGGAACGCGCCGCCGTGGAACGGAGGGAAGCCCAGGCCGTAGACCAgcg < 2:763177/142‑1 (MQ=255) aggcggACCTTCCGGCCCTTCATACAGCGGGCCGAGGTGCTGATATTGCTGTGCCATATCGAGGTATTTTGCGCTACCGAGGGTGTCCAGCCAGCGGAACGCGCCGCCGTGGAACGGAGGGAAGCCCAGGCCGTAGACCAgcg < 2:682538/138‑1 (MQ=255) aGACCTTCCGGCACTTCATACAGCGGGCCGAGGTGCTGATATTGCTGTGCCATATCGAGGTATTTTGCGCTACCGAGGGTGTCCAGCCAGCGGAACGCGCCGCCGTGGAACGGAGGGACGCCCCGCCCGCAGACCAGAGccct > 2:176952/1‑141 (MQ=255) aCTTCATACAGCGGGCCGAGGTGCTGATATTGCTGTGCCATATCGAGGTATTTTGCGCTACCGAGGGTGTCCAGCCAGCGGACCGCGCCGCCGTGGAACGGAGGGCAGCCCAGGCCGTAGTCCCGCGCCATATCCGCTTccgc > 1:252967/1‑143 (MQ=255) ttCATACAGCGGGCCGAGGTGCTGATATTGCTGTGCCATATCGAGGTGTTTTGCGCTACCGAGGGTGTCCAGCCAGCGGAACGCGCCGCCGTGGAACGGAGGGAAGCCCAGGCCGTAGACCGGCGGCATGACCGCGTccgccg > 2:474147/1‑143 (MQ=255) ttCATACAGCGGGCCGAGGTGCTGATATTGCTGTGCCATATCGAGGTATTTTGCGCTACCGAGGGTGTCCAGCCAGCGGAACGCGCCGCCGTGGAACGGAGGGAAGCCCAGGCCGTAGACCAGCGCCATACCCGCTTccgccg > 2:435765/1‑143 (MQ=255) gggCCGAGGTGCTGATATTGCTGTGCCATATCGAGGTATTTTGCGCTACCGAGGGTGTCCCGCCCGCGGAACGCGCCGCCGTGGAACGGAGGGAAGCCCCGGCCGTCGCCTCGCGCCGTATGTGCTTCCGCCGGTgcggcgtt > 1:232280/1‑141 (MQ=255) ccGAGGTGCTGATATTGCTGTGCCATATCGAGGTATTTCGCGCTACCGAGGGTGTCCAGCCAGCGGAACGCGCCGCCGTGGAACGGAGGGAAGCCCAGGCCGTAGACCAGCGCCATATCCGCTCCCGCCGGAGTGGCGATAAt > 2:228092/1‑143 (MQ=255) ggTGCTGATCTTGCTGTGCCATATCGAGGTACTTTGCGCTACCGAGGGTGTCCAGCCAGCGGAACGCGCCGCCGTGGAACGGAGGGAAGCCCAGGCCGTAGACCAGCGCCATATCCGCTTCCGCCGGAGTGGCGATAATGCCt < 1:193802/143‑1 (MQ=255) gCTGATATTGCTGTGCCATATCGAGGAATTTTGCGCTACCGAGGGTGTCCAGCCAGCGGAACGCGCCGCCGTGGAACGGAGGGAAGCCCAGGCCGTAGACCAGCGCCATATCCGCTTCCGCCGGAGTGGCGATAATGCCTtcc > 2:528564/1‑143 (MQ=255) cTGATATTGCTGTGCCATATCGAGGTATTTTGCGCTACCGAGGGTGTCCAGCCAGCGGAACGCGCCGCCGAGGAACGGAGGGAAGCCCAGGCCGTAGACCAGCGCCATATCCGCTTCCGCCGGAGTGGCGAGAATGCCTtccg > 1:167080/1‑142 (MQ=255) tatTGCTGTGCCATATCGAGGTATTTTGCGCTACCGAGGGTGTCCAGCCAGCGGAACGCGCCGCCGTGGAACGGAGGGAAGCCCAGGCCGTAGACCAGCGCCATATCCGCTTCCGCCGGAGTGGCGATAATGCCTTCCTCCAg > 1:633765/1‑143 (MQ=255) gCTGTGCCATATCGAGGTATTTTGCGCTACCGAGGGTGTCCAGCCAGCGGAACGCGCCGCCGTGGAACGGAGGGAAGCCCAGGCCGTAGACCAGCGCCATATCCGCTTCCGCCGGAGTGGCGATAATGCCTTCCTccagccag > 1:11018/1‑143 (MQ=255) tgtgCCATATCGAGGTATTTTGCGCTACCGAGGGTGTCCAGCCAGCGGAACGCGCCGCCGTGGAACGGAGGGAAGCCCAGGCCGTAGACCAGCGCCATATCCGCTTCCGCCGGAGTGGCGATAATGCCTTCCTCCAGACAgcg > 1:560380/1‑143 (MQ=255) | CATTGTGACTCCTTAAGCCGTTTTCAGGTCGCCAACCGGACGGGCTGGCTCAACCGGAGGATAGTACGGTTCGTTATGACGCGCTTTATTACGCAGACCTTCCGGCACTTCATACAGCGGGCCGAGGTGCTGATATTGCTGTGCCATATCGAGGTATTTTGCGCTACCGAGGGTGTCCAGCCAGCGGAACGCGCCGCCGTGGAACGGAGGGAAGCCCAGGCCGTAGACCAGCGCCATATCCGCTTCCGCCGGAGTGGCGATAATGCCTTCCTCCAGACAGCG > NC_000913/4028770‑4029051 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |