| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_000913 | 4,123,110 | G→A | 100% | P77L (CCA→CTA) | ftsN ← | cell division protein FtsN |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 4,123,110 | 0 | G | A | 99.0% | 83.4 / ‑6.8 | 35 | P77L (CCA→CTA) | ftsN | cell division protein FtsN |
| Reads supporting (aligned to +/- strand): ref base G (0/0); major base A (16/17); minor base C (1/1); total (17/18) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
| Rejected as polymorphism: E-value score below prediction cutoff. | |||||||||||
| Rejected as polymorphism: Frequency below/above cutoff threshold. | |||||||||||
| Rejected as polymorphism: Variant not supported by required number of reads on each strand. | |||||||||||
GTTCAAGAAGCTGACGTTGTTCTGGTGTCAGTTGCTCCGGCGTTTTCACTTCACCACCGGCAGAAGGTTCTGTGGGCGCACGCACTCCCGGCTGGCGACTTTCCAGCTCTTTAATGTAGCGCCAGCGTTCTTCTGGTTTTGGTGGTAGTCCGTTTCCGGTCACTTTCTGGCTTTGCAGCGTCTCGGACTCTTCTTTCTTGTGATGCGTAATGAAGTACAGACCACCGATAAAGGTCACAAGAACGGCGGCAGCAATAGCGACCATAGCGGGA > NC_000913/4122970‑4123241 | gTTCAAGAAGCTGACGTTGTTCTGGTGTCAGTTGCTCCGGCGTTTTCACTTCACCACCGGCAGAAGGTTCTGTGGGCGCACGCACTCCCGGCTGGCGACTTTCCAGCTCTTTAATGTAGCGCCAGCGTTCTTCTGGTTTtagt < 2:846330/143‑1 (MQ=255) ttCAAGAAGCTGACGTTGTTCTGGTGTCAGTTGGTCCGGCGTTGTCACTTCACCACCGGCTGAAGGTTCTGTGGGCGCGCGCGCTCCGGGCTGGCGACGTTCCAGCTCTTGAATGTAGCGCCAGCGTTCTTCTGGTTTtagtg > 2:702986/1‑143 (MQ=255) ttCAAGAAGCTGACGTTGTTCTGGTGTCAGTTGCTCCGGCGTTTTCACTTCACCACCGGCAGAAGGTTCTGTGGGCGCACGCACTCCCGGCTGGCGCCTTTCCAGCTCTTTAATGTAGCGCCAGCGCTCCTCTGGTTTtagtg > 2:238262/1‑143 (MQ=255) ttCAAGAAGCTGACGTTGCTCTGGTGTCAGTTGCTCCCGCGTTTTCACTTCCACACCGGCCGAAGGTTCTGTGGGCCCACGCACTCCCGGCTGGCCAGTTTTTATTCCTTTTATGTAGCGCCAGCGTTCTTCTGGTTTtagtg < 2:316377/143‑1 (MQ=255) aagaagCTGACGTTGTTCTGGTGTCAGTTGCTCCGGCGTTTTCACTTCACCACCGGCAGAAGGTTCTGTGGGCGCACGCACTCCCGGCTGGCGACTTGCCAGGTCTTTAATGTAGCGCCCGCGTGCATCGGGTGTTAGGGGTa > 2:842083/1‑143 (MQ=255) agCTGACGTTGTTCTGGTGTCAGTTGCTCCGGCGTTTTCACTTCACCACCGGCAGAAGGTTCTGTGGGCGCACGCACTCCCGGCTGGCGACTTTCCAGCTCTTTAATGTAGCGCCAGCGTTCTTCTGGTTTTAGTGGTAGTcc < 1:395167/143‑1 (MQ=255) gCTGACGTTGTTCTGGTGTCAGTTGCTCCGGCGTTTTCACTTCACCACCGGCAGAAGGTTCTGTGGGCGCACGCACTCCCGGCTGGCGACTTTCCAGCTCTTTAATGTAGCGCCAGCGTTCTTCTGGTTTTAGTGGTAGTCCg > 1:524140/1‑143 (MQ=255) gACGTTGTTCTGGTGACAGTTGCTCAGGCACTGGCACGCCACCACCGGCAGAAGGTTCTGTGGGCGCACGCACTCCCGGCTGGCGACTCTCCAGCTCTTTAACGTCGCGCCAGCGTCCTTCTGGTTTCCGTGGTAGTCCGttt > 2:275551/1‑143 (MQ=255) gttgttGTGGTGTCCGTTGCTCCGGGGTTTTCACTTCACCACCGGCAGAAGGTTCTGTGGGCGCACACACTCCCGGCTGGCGACTTTCCAGCTCTTTAATGTAGCGCCAGCGTTCTTCTGGTTTTAGTGGTAGGCCGTTTCCg < 2:857131/143‑1 (MQ=255) tCTGGTGTCAGTTGCTCCGGCGTTTTCGCTGCGCCTGCGGCAGAGGGTTCTGTGGGCGCACGCACTCCCGGCTGGCGACTTTCCAGCTCTTTAATGTAGCGCCAGCGTTCTTCTGGTTTTAGTGGTAGTCCGTTTCCGGTCAc < 2:331683/143‑1 (MQ=255) ttGCTCCGGCGTTTTCACTTCACCACCGGCAGAAGGTTCTGTGGGCGCACGCACTCCCGGCTGGCGACTTTCCAGCTCTTTAATGTAGCGCCAGCGTTCTTCTGGTTTTAGTGGTAGTCCGTTTCCGGTCACTTTCTGGCttt > 1:808965/1‑143 (MQ=255) cTCCGGCGTTTTCACTTCACCACCGGCAGAAGGTTCTGTGGGCGCACGCACTCCCAGCTGGCGACTATCCAGCTCTTTAGTGTAGCGCCAGCGTTCTTCTGGTTTTAGTGGTAGTCCGTTTCGGGTCACTTACTTGCTTTGCa > 1:61051/1‑143 (MQ=255) ccGGCGTTTTCACTTCACCACCGGCAGAAGGTTCTGTGGGCGCACGCACTCCCGGCTGGCGACTTTCCAGCTCTTTAATGTAGCGCCAGCGTTCTTCTGGTTTTAGTGGTAGTCCGTTTCCGGTCACTTTCTGGCTTTGCAGc > 1:487688/1‑143 (MQ=255) cGTTTTCACTTCACCACCGGCAGAAGGTTCTGTGGGCGCACGCACTCCCGGCTGGCGACTTTCCAGCTCTTTAATGTAGCGCCAGCGTTCTTCTGGTTTTAGTGGTAGTCCGTTTCCGGTCCCCTTCTGGCTTCGCACCGcct > 1:171279/1‑143 (MQ=255) ttttCACTTCACCACCGGCAGAAGGTTCTGTGGGCGCACGCACTCCCGGCTGGCGACTTTCCAGCTCTTTAATGTAGCGCCAGCGTTCTTCTGGTTTTAGTGGTAGTCCGTTTCCGGTCACTTTCTGGCTTTGCAGCGTCTCg < 1:458389/143‑1 (MQ=255) ttcacttcacCACCGGCAGAAGGTTCTGTGGGCGCACGCACTCCCGGCTGGCGACTTTTCAGCGCTTTAATGTAGCGCCAGCGTTCTTCTGGTTTTAGTGGTAGTCCGTTTCCGGTCACTTTCTGGCTTTGCAGCGTCTCGGa > 1:697817/1‑143 (MQ=255) cctcggccaccGGCAGAAGGGTCTGTGGGCGCACGCGCTCCCGGCTGGCGACTTTCCAGCTCTTTAATGTGGCGCCAGCGTTTTTCTGGTTTGAGTGGTAGTCCGTTTCCGGTCACTTTCTGGCTTTGCAGCGTCTCGGActc < 2:85296/137‑1 (MQ=255) ttcacCACCGGCAGAAGGTTCTGTGGGCGGACGCACTCCCGACTGGGGACTTTCCAGCTCTTCAATGTAGCGCCAGCGTTCGTCTGGTTTTAGTGGTAGTCCGTTTCCGGTGACAATTCGGATTTGCAGAGTCTCGGATtctt > 2:218592/1‑143 (MQ=255) accGGCAGAAGGTTCTGTGGGCGCACGCACTCCCGGCTGGCGACTTTCCAGCTCTTTAATGTAGCGCCAGCGTTCTTCTGGTTTTAGTGGTAGTCCGTTTCCGGTCACTTTCTGGCTTTGCAGCGTCTCGCCCTCttctttct > 1:583900/1‑143 (MQ=255) cGGCAGGAGGTTCGCTGGGCGCGCGCTCGCCCGGCTGGCGACTGTCCAGCGCTTTAATGTAGCGCCAGCGTTCTTCTGGTTTTAGTGGTAGTCCGTTTCCGGTCACTTTCTGGCTTTGCAGCGTCTCGGACTCTTCTTTCTtg < 2:752572/143‑1 (MQ=255) ctgtacgTTCTGTTGGCGCACGCACTGCCGGTTGTGGACTTTTCAGCTCTTTTTTGTGGCGCTAGCGTTCTTCTGTTTTTCGTCGTACTCCGTTTCCTGTCACTTTCTGGCTTTGCAGCGTCTCGGACTCTTCTTTCTTGTGa < 2:125367/137‑1 (MQ=255) cAGAAGGTGCTGTGGGCGCACGCACCCCCGGCTGGCGACTTTCCAGCTCTTTCATGTAGCGCCAGCGTTCTTCTGGTTTTAGTGGTAGTCCGTTTCCGGTCACTTTCTGGCTTTGCAGCGTCTCGGACTCTTCTTTCTTGTGa < 1:238262/143‑1 (MQ=255) gtgGGCGCACGCACTCCCGGCTGGCGACTTTCCAGCTCTTTAATGTAGCGCCAGAGGTGGGCCGGTGGTACTGGTCGTCCGTTTCCGGTCACTTTCTGGCTTTGCAGCGTCTCGGACTCTGCTTTCTTGTGCTGCGTAATGac > 1:847858/1‑142 (MQ=255) gggggCGCACGGGCGCCCGGCTGGCGGCTTGCCGGCGCTTTGGTGTGGCGCCAGCGTTCTTCTGGTTTTAGTGGTAGTCCGTTTCCGGTCACTTTCTGGCTTTGCGGCGTCTCGGACTCTTCTTTCTTGTGATGCGTAATGaa < 2:266446/141‑1 (MQ=255) ggCGCACGCGGTGCCGTCTGGCGACTTTCCGGCTCTTTAATGTAGCGCCAGCGTTCTTCTGGTTTTAGTGGTAGTCCGTTTCCGGTCACTTTCTGGCTTTGCAGCGTCTCGGACTCTTCTTTCTTGTGATGCGTAATGAAGTa < 2:527358/143‑1 (MQ=255) gcacgcacTCCCGGCTGGCGACTTTCCAGCTCTTTAATGTAGCGCCAGCGTTCTTCTGGTTTTAGTGGTAGTCCGTTTCCGGTCACTTTCTGGCTTTGCAGCGTCTCGGACTCTTCTTTCTTGTGATGCGTAATGAAGTACAg < 1:348259/143‑1 (MQ=255) cgcacTCCCGGCTGGCGACTTTCCAGCTCTTTAATGTAGCGCCAGCGTTCTTCTGGTTTTAGTGGTAGTCCGTTTCCGGTCACTTTCTGGCTTTGCAGCGTCTCGGACTCTTCTTTCTTGTGATGCGTAATGAAGTCCAGacc > 2:358761/1‑143 (MQ=255) ggCTGGCGACTTTCCAGCTCTTTAATGTGGCGCCAGCGTTCTTCTGGTTTTAGTGGTAGTCCGTTTCCGGTCACTTTCTGGCTTTGCAGCGTCTCGGACTCTTCTTTCTTGTTATGCGTAATGAAGTACAGACCACCGATaaa < 2:601632/143‑1 (MQ=255) ggCGACTTTCCAGCTCTTTAATGTAGCGCCAGCGGTCTTCTGGTTTTAGTGGTAGTCCGTTTCCGGTCACTTTCTGGCTTTGCAGCGTCTCGGACTCTTCTTTCTTGTGATGCGTAATGCAGTACAGACCACCGGTAAAGGTc > 1:152059/1‑143 (MQ=255) ttCCAGCTCTTTAATGTAGCGCCAGCGTTCTTCTGGTTTTAGTGGTAGTCCGTTTCCGGTCACTTTCTGGCTTTGCAGCGTCTCGGACTCTTCTTTCTTGTGATGCGTAATGAAGTACAGACCACCAATAAAGGTCAaaaaaa > 1:521412/1‑143 (MQ=255) ccAGCTCTTTAATGTAGCGCCAGCGTTCTTCAGGTATTAGCGGCACTCCCTTTCCGGTCACTTTCTGGCTTTGCAGCGTCGCGGACTCTTCTTTCTTGTGATGCGTAATGAAGTACAGACCACCGATAGAGGGCACAAGAAcg > 1:896904/1‑143 (MQ=255) aGCTCTTTAATGTCGCGCCAGCGCTCTTCTGGTTTTAGTGGTAGTCCGTTTCCGGTCACTTTCTGGCTTTGCAGCGTCTCGGACTCTTCTTTCTTGTGATGCGTAATGAAGTACAGACCACCGATAAAGGTCACAAGAAcggc < 1:388209/143‑1 (MQ=255) tAATGTAGCGCCAGCGTTCTTCTGGTTTTAGTGGTAGTCCGTTTCCGGTCACTTTCTGGCTTTGCAGGGTCTCGGACTCTTCTTTCTTGTGATGCGTGATGAAGTCGGGACCCCCGATAAAGGTCACCAGCAcggcgctagcc > 2:600828/1‑137 (MQ=255) ggTAGCGCCTGCGTTCTTCTTGTTTTAGTGGTAGTGCGTTTCCGTTCACTTTCTGGCTTTGCAGGGTCTCGGACTCTTCTTTCTTGTGATGCGTAATGAAGTACAGACCACCGATAAAGGTCACAAGAACGGCGGCAGCAATa < 2:75788/142‑1 (MQ=255) gTAGCGCCAGCGTTCTTCTGGTTTTAGTGGTAGTCCGTTTCCGGTCACTTTCTGGCTTTGCAGCGTCTCGGACTCTTCTTTCTTGTGATGCGTAATGAAGTACAGACCACCGATAAAGGTCACAAGAACGGCGGCAGCAATAg < 1:759610/143‑1 (MQ=255) gcgcCAGCGTTCTTCTGGTCTTAGTAGTAGTTCGATGCGGGTCACTTTCTGCCTTTGCAGCTTCTCGGACTCTTCTTTCTTGTGATGCGTAATGAAGTACAGACCACCGATAAAGGACACAAGAACGGCAGCAGCAATAGCGa > 2:492585/1‑143 (MQ=255) gcgcCAGCGTTCTTCTGGGTTTAGTGGTAGTCCGTTTCCGGTCACTTTCTGGCTTTGCAGCGTCTCGGACGCTTCTTTCTTGTGATGCGTAATGAAGTACAGACCACCGATAAAGGTCACAAGAACGGCGGCAGCAATAGCGa < 1:304498/143‑1 (MQ=255) tttctGGTTTGAGTGGTAGTCCGTTTCCGGTCACTTTCTGGCTTTGCAGCGTCTCGGACTCTTCTTTCTTGTGATGCGTAATGAAGTACAGACCACCGAGAAAGGTCACAAGAACGGCGGCAGCAATAGCGACCATAGCGGga < 2:164856/142‑1 (MQ=255) cttgtGGTTTTAGTGGTAGTCTGTGTCCGGTCGCTTTCTGGTGTTGCAGCGTCTCGGACTCTTCTTTCTTGTGATGCGTAATGAAGTACAGACCACCGATAAAGGTCACAAGAACGGCGGCAGCAATAGCGACCATAGCGGga < 2:362114/143‑1 (MQ=255) | GTTCAAGAAGCTGACGTTGTTCTGGTGTCAGTTGCTCCGGCGTTTTCACTTCACCACCGGCAGAAGGTTCTGTGGGCGCACGCACTCCCGGCTGGCGACTTTCCAGCTCTTTAATGTAGCGCCAGCGTTCTTCTGGTTTTGGTGGTAGTCCGTTTCCGGTCACTTTCTGGCTTTGCAGCGTCTCGGACTCTTCTTTCTTGTGATGCGTAATGAAGTACAGACCACCGATAAAGGTCACAAGAACGGCGGCAGCAATAGCGACCATAGCGGGA > NC_000913/4122970‑4123241 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |