Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 4,426,592 T→A 9.1% intergenic (+35/+36) rplI → / ← yjfZ 50S ribosomal subunit protein L9/protein YjfZ

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,426,5920TA 9.1% 149.4 / 7.9 55intergenic (+35/+36)rplI/yjfZ50S ribosomal subunit protein L9/protein YjfZ
Reads supporting (aligned to +/- strand):  ref base T (22/28);  new base A (2/3);  total (24/31)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.79e-02

TTCGTCTGCCGAACGGCGTTCTGCGTACCACTGGCGAACACGAAGTGAGCTTCCAGGTTCACAGCGAAGTATTCGCGAAAGTGATCGT‑AAACGTAGTAGCTGAATAATTCGTTATTCAACGAGACGTAAAAACGCCGACCATTGGTCGGCGTTTTGCTTTCTA‑TTTTTCGTCAGGTATTAGTTTCGCAAGTAGATCCATTCACCATTCTCTTGTGTCCATAAGTCAGGATGGTTTGCTGGCGTTAGATACATACGAGCGCAACTGATGTCTCGCTCCTGC  >  NC_000913/4426451‑4426730
                                                                                                                                              |                                                                                                                                           
ttCGTCTGCCGAACGGCGTTCTGCGTACCACTGGCGAACACGAAGTGAGCTTCCAGGTTCACAGCGAAGTATTCGCGAAAGTGATCGT‑AAACGTAGTAGCTGAATAATTCGTTATTCAACGAGACGTAAAAACGCCGACCAtt                                                                                                                                            <  1:490014/143‑1 (MQ=255)
   gTCTGCCGAACGGCGTTCTGCGTACCACTGGCGAACACGAAGTGAGCTTCCAGGTTCACAGCGAAGTATTCGCGAAAGTGATCGT‑AAACGTAGTAGCTGAATAATTCGTTATTCAACGAGACGTAAAAACGCCGACCATTGGt                                                                                                                                         <  1:519536/143‑1 (MQ=255)
       gtcGAACGGACTGCTACGTACCACTGGCGAACACGAAGTGAGCTTCCATGTGCACAGCTTAGTATTCGCGAAAGTGATCGT‑AAACGTAGTAGCTGAATAATTCGTTATTCAACGAGACGTAAAAACGCCTACCATTGGTCGGc                                                                                                                                     <  2:180842/141‑1 (MQ=255)
       gCCGAACGGCGTTCTGCGTACCACTGGCGAACACGAAGTGAGCTTCCAGGTTCACAGCGAAGTATTCGCGAAAGTGATCGT‑AAACGTAGTAGCTGAATAATTCGTTATTCAACGAGACGTAAAAACGCCGACCAATGGTCGGc                                                                                                                                     <  1:830665/143‑1 (MQ=255)
       gCCGAACGGCGTTCTGCGTACCACTGGCGAACACGAAGTGAGCTTCCAGGTTCACAGCGAAGTATTCGCGAAAGTGATCGT‑AAACGTAGTAGCTGAATAATTCGTTATTCAACGAGACGTAAAAACGCCGACCAATGGTCGGc                                                                                                                                     <  1:452263/143‑1 (MQ=255)
        ccGAACGGCGTTCTGCGTACCACTGGCGAACACGAAGTGAGCTTCCAGGTTCACAGCGAAGTATTCGCGAAAGTGATCGT‑AAACGTAGTAGCTGAATAATTCGTTATTCAACGAGACGTAAAAACGCCGACCATTGGTCGGCg                                                                                                                                    >  1:528555/1‑143 (MQ=255)
          gAACGGCGTTCTGCGTACCACTGGCGAACACGAAGTGAGCTTCCAGGTTCACAGCGAAGTATTCGCGAAAGTGATCGT‑AAACGTAGTAGCTGAATAATTCGTTATTCAACGAGACGTAAAAACGCCGACCAATGGTCGGCGtt                                                                                                                                  <  2:745604/143‑1 (MQ=255)
          gAACGGCGTTCGGCGTACCACTGGCGAACAGGAAGTGAGCTTCCAGGTTCACAGCGAAGTATTCGCGAAAGTGATCGT‑AAACGTAGTAGCTGAATAATTCGTTATTCAACGAGACGTAAAAACGCCGACCATTGGTCGGCGtt                                                                                                                                  <  2:75596/143‑1 (MQ=255)
            aCGGCGTTCTGCGTACCACTGGCGAACACGACGTGAGCTTCCAGGTTCACAGCGAAGTATTCGCGAAAGTGATCGT‑AAACGTAGTAGCTGAATAATTCGTTATTCAACGAGACGTAAAAACGCCGACCATTGGTCGGCGtttt                                                                                                                                <  1:822211/143‑1 (MQ=255)
             gcgccttCTGCGTACCACTGGTGAACTCGAAGTGAGCTGCCGGGTTCAGAGGGCAGTACTCGGGAAAGTGATCGT‑AAACGTACTAGCGGAATAATTCGTTATTCAACGAGACGTAAAAACGCCGACCATTGGTCGGCGTTTTg                                                                                                                               <  2:325374/138‑1 (MQ=255)
             cGGCGCTCTGCGTACCACTGGCGAACACGAAGTGAGCTTCCAGGTTCACAGCGAAGTATTCGCGAAAGTGATCGT‑AAACGTAGTAGCTGAATAATTCGTTATTCAACGAGACGTAAAAACGCCGACCATTGGTCGGCGTTTTg                                                                                                                               <  1:772846/143‑1 (MQ=255)
               gCGTTCTGCGTACCACTGGCGAACACGAAGTGAGCTTCCAGGTTCACAGCGAAGTATTCGCGAAAGTGATCGT‑AACCGTAGTCGCTGAATCATTCGTTCTCCAACGAGACGTAAAAACGCCGCCCATTGATCCGCGTTTTGCt                                                                                                                             >  1:23225/1‑143 (MQ=255)
                 gTTCGGCCTACCCCTGGCGAACACGAAGTGAGCTTCCAGGTTCACAGCGAAGTATTCGCGAAAGTGATCGT‑AAACGTAGTAGCTGAATAATTCGTTATTCAACGAGACGTAAAAAAGCCGAGCATTGGTCGGCGTTTTGCttt                                                                                                                           <  2:319478/143‑1 (MQ=255)
                            cACTGGCGAACACGAAGTGAGCTTCCAGGTTCACAGCGAAGTATTCGCGAAAGTGATCGT‑AAACGTAGTAGCTGAATAATTCGTTATTCAACGAGACGTCAAAACGCAGACAGTCCAACTGCGTATTGCTTTCTA‑TTTTTCGt                                                                                                               >  1:557051/1‑143 (MQ=255)
                            cACTGGCGAACACGAAGTGAGCTTCCAGGTTCACAGCGAAGTATTCGCGAAAGTGATCGT‑AAACGTAGTAGCTGAATAATTCGATATTCAACGAGACGTAAAAACGCCGACCATTGGTCGGCGTTTTGCTTTCTG‑TTTTTCGt                                                                                                               >  2:285250/1‑143 (MQ=255)
                             aCTGGCGAACACGAAGTGAGCTTCCAGGTTCACAGCGAAGTATTCGCGAAAGTGATCGT‑AAACGTAGTAGCTGAATAATTCGTTATTCAACGAGACGTAAAAACGCCGACCATTGGTCGGCGTATGGCTTTCTA‑ttttatgta                                                                                                              >  2:209456/1‑138 (MQ=255)
                               tGGCGAACACGAAGTGAGCTTCCAGGTTCACAGCGAAGTATTCGCGAAAGTGATCGT‑AAACGTAGTAGCTGAATAATTCGTTATTCAACGAGACGTAAAAACGCCGACCATTGGTCGGCGTTTTGCTTTCTATTTTTTCGTCa                                                                                                             >  1:669589/1‑143 (MQ=255)
                                 gCGAACACGAAGTGAGCTTCCAGGTTCACAGCGAAGTATTCGCGAAAGTGATCGT‑AAACGTAGTAGCTGAATAATTCGTTATTCAACGGGGCGTAAAAACGCCGACCATTGGTCGGCGTGTTGCTTTCTA‑TTTTTCGTCAGGt                                                                                                          >  2:542787/1‑143 (MQ=255)
                                 gCGAACACGAAGTGAGCTTCCAGGTTCACAGCGAAGTATTCGCGAAAGTGATCGT‑AAACGTAGTAGCTGAATAATGCGTTATTCAACGAGACGTAAAAACGCCGACCATTGGTCGGCGTTTTGCTTTCTA‑TTTTTCGTCAGGt                                                                                                          <  1:311089/143‑1 (MQ=255)
                                          cattgtgCTTGCAGGTTCACAGCGAAGTATTCGCTAAAGTGATCGT‑AAACGTAGTAGCTGAATAATTCGTTATTCAACGAGACGTAAAAACGCCGACCATTGGTCGGCGTTTTGCTTTCTA‑TTTTTCGTCAGGTATTAGTTTc                                                                                                 <  2:832860/137‑1 (MQ=255)
                                            gTGAGCTTCCAGGTTCACAGCGAAGTATTCGCGAAAGTGATCGT‑AAACGTAGTAGCTGAATAATTCGTTATTCAACGAGACGTAAAAACGCCGACCATTGGTCGGCGTTTTGCTTTCTA‑TTTTTCGTCAGGTATTAGTTTCGc                                                                                               >  1:324785/1‑143 (MQ=255)
                                                gCTTCCAGGTTCACAGCGAAGTATTCGCGAAAGTGATCGT‑AAACGTAGTAGCTGAATAATTCGTTATTCAACGAGACGTAAAAACGCCGACCATTGGTCGGCGTTTTGCTTTCTA‑TTTTTCGTCAGGTATTAGTTTCGCAAGt                                                                                           >  1:299271/1‑143 (MQ=255)
                                                 cTTCCAGGTTCACAGCGAAATATGCGCGAAAGTGATCGT‑AAACGTAGTAGCTGAATAATTCGTTATTCAACGAGACGTAAAAACGCCGACCATTGGTCGGCGTTTTGCTTTCTA‑TTTTTCGTCAGGTATTAGTTTCGCAAGTa                                                                                          <  2:504504/143‑1 (MQ=255)
                                                  ttCCAGGTTCACAGCGAAGTATTCGCGAAAGTGATCGT‑AAACGTAGTAGCTGAATAATTCGTTATTCAACGAGACGTAAAAACGCCGACCATTGGTCGGCGTTTTGCTTTCTA‑TTTTTCTTCAGGTATTAGTTTCGCAAGTAg                                                                                         >  2:672349/1‑143 (MQ=255)
                                                    ccAGGTTCACAGCGAAGTATTCGCGAAAGTGATCGT‑AAACGTAGTAGCTGAATAATTCGTTATTCAACGAGACGTAAAAACGCCGACCATTGGTCGGCGTCTTGCTTTCTA‑TTTTTAGTCAGGGATTAGTTTCGCCAgccgat                                                                                       >  1:481673/1‑138 (MQ=255)
                                                      aGGTTCACAGCGAAGTATTCGCGAAAGTGATCGT‑AAACGTAGTAGCTGAATAATTCGTTATTCAACGAGACGTAAAAACGCCGACCATTGGTCGGCGTTTTGCTTTCTA‑TTTTTCGTCAGGTATTAGTTTCGCAAGTAGATcc                                                                                     >  1:158044/1‑143 (MQ=255)
                                                         ttCACAGCGAAGTATTCGCGAAAGTGATCGT‑AAACGTAGTAGCTGAATAATTCGTTATTCAACGAGACGTAAAAACGCCGACCATTGGTCGGCGTTTTGCTTTCTA‑TTTTTCGTCAGGTATTAGTTTCGCAAGTAGATCCAtt                                                                                  >  1:795604/1‑143 (MQ=255)
                                                           cacaGCGAAGTATTCGGTAAAGTGATCGT‑AAACGTAGTAGCTGAATAATTCGTTATTCAACGAGACGTAAAAACGCCGACCATTGGTCGGCGTTTTGCTTTCTA‑TTTTTCGTCAGGTATTAGTTTCGCAAGTAGATCCATTCa                                                                                <  1:857555/143‑1 (MQ=255)
                                                                   ggTATTCGC‑AAAGTGGTCCTGAAGCGTGGGAGCTGAATAATTCGTTATTCTACGAGACGTAAAATCGCCGACCATTGGTCGGCGTTTTGATTTTCA‑TTTTTCGTCAGGTATTAGTTTCGCAAGTAGATCCATTCACCATtctc                                                                        <  2:728790/142‑1 (MQ=255)
                                                                   atgatgcgcgAAAGTGATCGC‑AAACGTAGTAGCTGAATAATTCGTTATTCAACGAGACGTAAAAACGCCGACCATTGGTCGGCGTTTTGCTTTCTA‑TTTTTCGTCAGGTATTAGTTTCGCAAGTAGATCCATTCACCATtctc                                                                        <  1:735219/137‑1 (MQ=255)
                                                                     gaTTCTCGAAAGTGTCCGT‑AATCGTAGTGGCTGAATAAGTCGTTATTCAACGATACGTAAATACGCCGACCATTGGTCTGCGTTTTGCTTTCTA‑TTTTTCGTCAGGTATTAGTTTCTCAGGTAGATCCATTCACCATTCTCtt                                                                      <  2:21985/142‑1 (MQ=255)
                                                                       ttCGCGAAAGTGATCGT‑AAACGTAGTAGCTGAATAATTCGTTATTCACCGAGACCACAAAACGCCGACCATTGGTCGGCGTTTTGCTTTCTA‑TTTTTCGTCAGGTATTAGTTTCGCAAGTAGATCCATTCACCATTCTCTtgt                                                                    <  1:650746/143‑1 (MQ=255)
                                                                       ttCGCGAAAGTGATCGT‑AAACGTAGTAGCTGAATAATTCGTTATTCAACGAGACGTAAAAACGCCGACCATTGGTCGGCGTTTTGCTTTCTA‑TTTTTCGTCAGGTATTAGTTTCGCAAGTAGATCCATTCACCATTCTCTtgt                                                                    <  2:112256/143‑1 (MQ=255)
                                                                       tgcacgAAAGTGATCGT‑AAACGTAGTAGCTGAATAATTCGTTATTCAACGAGACGTAAAAACGCCGACGATTGGTCGGCGTTTTGCTTTCTA‑TTTTTCGTCAGGTATTAGTTTCGCAAGTAGATCCATTCACCATTCTCTtgt                                                                    <  2:505910/139‑1 (MQ=255)
                                                                          gcgAAAGTGATCGT‑AAACGAAGTAGCTGAATAATTCGTTATTCAACGAGACGTAAAAACGCCGACCATTAATCAGCATTTTGCTTTCTA‑TTTTTAGTCAGGAATTAGTTTCACAAGTAAATCCAATCACCATTCTCTTGTATc                                                                 >  2:451026/1‑143 (MQ=255)
                                                                           cgAAAGTGATCGT‑AAACGTAGTAGCTGAATAATTCGTTATTCAACGAGACGTAAAAACGCCGACCATTGGTCGGCGTTTTGCTTTCTA‑TTTTTCGTCAGGTATTAGTTTCGCAAGTAGATCCATTCACCATTCTCTTGTGTcc                                                                <  1:373365/143‑1 (MQ=255)
                                                                                  gATCGT‑AAACGTAGTATCTGAATAATTCGTTATTCAACGAGACGTAAAAACGCCGACCATTGGTCGGCGTTTTGCTTTCTA‑TTTTTCGTCAGGTATTAGTTTCGCAAGTAGATCCATTCACCATTCTCTTGTGTCCATAAGTc                                                         <  2:546205/143‑1 (MQ=255)
                                                                                   gtCGT‑ATCCGTAGGAGCTGCATCATTCGTTATTCAACGAGACGTAAAGTCGCCGACCATTGGTCGGCGTTTTGCTTTCTA‑TTTTTCGTCAGGTATTAGTTTCGCAAGTAGATCCATTCACCGTTCTCTTGTGTCCATAAGTCa                                                        <  2:584690/142‑1 (MQ=255)
                                                                                     cGT‑AAACGTAGTAGCTGAATAATTCGTTATTCAACGAGACGTAAAAACGCCGACCATTGGTCGGCGTTTTGCTTTCTA‑TTTTTCGTCAGGTATTAGTTTCGCAAGTCGATCCATTCACCATTCTCTTGTGTCCATAAGTCAgg                                                      >  1:258808/1‑143 (MQ=255)
                                                                                      gT‑AAACGTAGTAGCTGAATAATTCGTTATTCAACGAGCCGTAAAAACGCCGACCATTGGTCGGCGTTTTGCTTTCTA‑TTTTTCGTCAGGTATTAGTTTCGCACGTAGATCCATTCACCCTTCTCTTGTGTCCATAAGTCATGa                                                     >  1:397568/1‑143 (MQ=255)
                                                                                               agtagCTGAATAATTCGTTATTCAACGAGACGTAAAAACGCCGACCATTGGTCGGCGTTTTGCTTTCTA‑TTTTTCGTCAGGTATTAGTTTCGCAAGTAGATCCATTCACCATTCTCTTGTGTCCATAAGTCAGGATGGTTTGc                                             <  1:734865/143‑1 (MQ=255)
                                                                                                 tagCTGAATAATTCGTTATTCAACGAGACGTAAAAACGCCGACCATTGGTCGGCGTTTTGCTTTCTA‑TTTTTCGTCAGGTATTAGTTTCGCAAGTAGATCCATTCACCATTCTCTTGTGTCCATAAGTCAGGCTGGTTTGCTg                                           >  1:833976/1‑143 (MQ=255)
                                                                                                   gCTGAATAATTCGTTATTCAACGAGACGTAAAAACGCCGACCATTGGTCGGCGTTTTGCTTTCTA‑TTTTTCGTCAGGTATTAGTTTCGCAAGTAGATCCATTCACCATTCTCTTGTGTCCATAAGTCAGGATGGTTTGCTGGc                                         <  1:624567/143‑1 (MQ=255)
                                                                                                         taatTCGTTATTCAACGAGACGTAAAAACGCCGACCATTGGTCGGCGTTTTGCTTTCTA‑TTTTTCGTCACGTATCAGTTTCGCAGGGAGATCCATTCACCATTCTCTTGTGTCCATGAGGCAGGATGGTTTGCTGGCGTgaga                                   >  1:148825/1‑143 (MQ=255)
                                                                                                         taatTCGTGCGTCAACGAGACGTAAAAACGCCGACCATTGGTCGGCGTTTTGCTTTCTA‑TTTGTCGTCAGGTATTAGTTTCGCAAGTAGATCCATTCACCATTCTCTTGTGTCCATAAGTCAGGATGGTTTGCTGGCGTTAGa                                   <  2:49111/143‑1 (MQ=255)
                                                                                                               gTTATTCAACCAGACGTAAAAACGCAGACCATAGGTCCGCGTTTTGCTTTCTA‑TTTTTCGTCAAGTATTAGTTTCGCAAGTAGATCCATTCAACATTCTCTTGTGTCCATAAGTCACGATGGCTTGCCAGCGTTAGacaccta                             >  2:115077/1‑143 (MQ=255)
                                                                                                                   ttCAACGAGACGTAAAAACGCCGACCATTGGTCGGCGTTTTGCTTTCTA‑TTTTTCGTCAGGTATTAGTTTCGCAAGTAGATCCATTCACCATTCTCTTGTGTCCATAAGTCAGGATGGTTTGCTGGCGTTAGATACATACGAg                         >  2:497218/1‑143 (MQ=255)
                                                                                                                        cGAGACGTAAAAACGCCGTCCATTGGTCGGCGTTTTGCTTTCTC‑TTTTTCGTCAGGTATTAGTTTCGCAAGTAGATCCATTCACCATTCTCTTGTGTCCATAAGTCAGGATGGTTTGCTGGCGTTAGATACATACGAGCGCaa                    <  2:463045/143‑1 (MQ=255)
                                                                                                                        cGAGACGTAAAAACGCCGACCATTGGTCGGCGTTTTGCTTTCTA‑TTTTTCGTCAGGTATTAGTTTCGCAAGTAGATCCATTCACCATTCTCTTGTGTCCATAAGTCAGGATGGTTTGCTGGCGTTAGATACATACGAGCGCaa                    <  2:105676/143‑1 (MQ=255)
                                                                                                                              gTAAAAACGCCGACCATTGGTCGGCGTTTTGCGTGCTA‑TTTTTCGTCAGGTATGAGTTTCGCAAGTAGATCCATTCACCATCCTCGTGTGTCCATAAGTCAGGATGGTTTGCTGGCGTTGGCGAGATACGAGCGCAACTGATg              <  2:784269/143‑1 (MQ=255)
                                                                                                                               tAACAACGCCGACCATTGGTCGGCGTTTTGCTTTCTA‑TTATCCGCCAGGTATTAGTTTCGCAGGCAGCTCCATCCACCATTCTCTTGTGTCCAT‑AGTCAGGATGGTTTGCTGGCGTTAGATACATCCGAGCGCAACTGAGGtc            >  2:691829/1‑143 (MQ=255)
                                                                                                                                  agccgccgcTCATTGGTCGGCGTTTTGCTTTCTA‑TTTTTCGTCAGGTATGAGGTTCGCAAGTAGATCCATTCACCTTTCTCTTGTGTCCATACGTCAGGATGGTTTGCTGGCGTTAGATACATACGAGCGCAACTGATGtctc          <  2:172801/140‑1 (MQ=255)
                                                                                                                                     cGCCGACCAATGGTCGGCGTTTTGCTTTCTA‑TTTTTCGTCAGGTATTAGTTTCGCAAGTAGATCCATTCACCATTCTCTTGTGTCCATCAGTCAGGATGGTTTGCTGGCGTTAGATACATACGAGCGCAACTGATGTCTCGCt       >  1:18613/1‑143 (MQ=255)
                                                                                                                                     cGCCGACCAATGGTCGGCGTTTTGCTTTCTA‑TTTTTCGTCAGGTATTAGTTTCGCAAGTAGATCCATTCACCATTCTCTTGTGTCCATAAGTCAGGATGGTTTGCTGGCGTTAGATACATACGAGCGCAACTGATGTCTCGCt       >  2:702665/1‑143 (MQ=255)
                                                                                                                                         gACCATTGGTCGGCGTTTTGCTTTCTA‑TTTTTCGTCAGGTATTAGTTTCGCAAGTAGATCCATTCACCATTCTCTTGTGTCCATAAGTCAGGATGGTTTGCTGGCGTTAGATACATACGAGCGCAACTGATGTCTCGCTCCTg   >  1:436270/1‑143 (MQ=255)
                                                                                                                                          aCCGTTGGTCGGCGTTTTGCTTTCTA‑TTTTTCTTCAGGTATTAGTTTCGCAAGTAGATCCATTCACCATTCTCTTGTGTCCATAAGTCAGGATGGTTTGCTGGCGTTAGATACATACGAGCGCAACTGATGTCTCGCTCCTGc  <  2:324785/143‑1 (MQ=255)
                                                                                                                                              |                                                                                                                                           
TTCGTCTGCCGAACGGCGTTCTGCGTACCACTGGCGAACACGAAGTGAGCTTCCAGGTTCACAGCGAAGTATTCGCGAAAGTGATCGT‑AAACGTAGTAGCTGAATAATTCGTTATTCAACGAGACGTAAAAACGCCGACCATTGGTCGGCGTTTTGCTTTCTA‑TTTTTCGTCAGGTATTAGTTTCGCAAGTAGATCCATTCACCATTCTCTTGTGTCCATAAGTCAGGATGGTTTGCTGGCGTTAGATACATACGAGCGCAACTGATGTCTCGCTCCTGC  >  NC_000913/4426451‑4426730

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑