| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_000913 | 285,887 | C→A | 6.8% | L165M (CTG→ATG) | yagG → | putative D‑xylonate transporter YagG |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 285,887 | 0 | C | A | 6.8% | 161.8 / 2.1 | 59 | L165M (CTG→ATG) | yagG | putative D‑xylonate transporter YagG |
| Reads supporting (aligned to +/- strand): ref base C (27/28); new base A (2/2); total (29/30) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.54e-01 | |||||||||||
CCTGGTCTACACCTTCGTTAACGTGCCGTACTGCGCCATGCCGGGCGTCATCACCGCCGACCCGAAAGAGCGTCACGCCCTGCAGTCCTGGCGCTTCTTCCTGGCGGCGGCGGGCTCGCTCGCTATCAGCGGCATCGCGCTGCCGCTGGTGAGCATCATCGGCAAAGGGGACGAGCAGGTGGGCTACTTCGGCGCCATGTGCGTGCTGGGGCTGAGCGGCGTGGTGCTGCTCTACGTCTGCTTCTTCACGACCAAAGAGCGCTACACCTTTGAGGTG > NC_000913/285748‑286024 | ccTGGTCTACACCTTCGTTAACGTGCCGTACTGCGCCATGCCGGGCGTCATCACCGCCGACCCGAAAGCGCGTCCCGCCCTGCAGTCCTGGCGCTTCTTCCTGGCGGCGGCGGGCTCGCTCGCGATCGGCGGCACCGCGCGGc > 2:854388/1‑143 (MQ=255)ccTGGTCTACACCTTCGTTAACGTGCCGTACTGCGCCATGCCGGGCGTCATCACCGCCGACCCGAAAGAGCATCGCGCCCTGCAGTCCTGGCGCTCCTTCCGCGCGGCGGCGCGCTCGCTCGCAATCCGCGCCATCGGGCCGc > 2:328725/1‑143 (MQ=255) ggTCGACACCTTCGTTAACGGGCAGTACTGCGCCACGCCGGGCGTCATCACCGCCGACCCGAAAGAGCGTCACGCCCTGCAGTCCTGGCGCTTCTTCCTGGCGGCGGCGGGCTCGCTCGCTATCAGCGGCAGCGCGATGCCGc < 1:428441/143‑1 (MQ=255) tcgtctccTTCGTTAGTGTGCCGTACTGCGCCATGCCGGGCGTCATCACCGCCGACCCGAAAGAGCGTCACTCCCTGCAGTTCTGGCGCTTCTTCCTGGCGGCGGCGGGCTCGCTCGCTATCAGCGGTAGCGCGATGCCGCTg < 2:687862/137‑1 (MQ=255) tCTACACCGTCGTTACCGTGCCGTACTGCGCCATGGCGGGCGTCATCACCGCCGACCCGAAAGAGCGTCACGCCCTGCAGTCCTGGCGCTTCTTCCTGGCGGCGGCGGGCTCGCTCGCTATCAGCGGCATCGCGCTGCCGCTg < 2:645906/143‑1 (MQ=255) cTACACCTTCGTTAACGTGCCGTACTGCGCCATGCCGGGCGTCATCACCGCCGACCCGAAAGAGCGTCACGCCCTGCAGTCCTGGCGCTTCTTCCTGGCGGCGGCGGGCTCGCTCGCTATCAGCGGGATCGcgctggcgctgg > 1:10040/1‑143 (MQ=255) tACACCTTCGTTAACGTGCCGTACTGCGCCATGCCGGGCGTCATCACCGCCGACCCGAAAGAGCGTCACGCCCTGCAGTCCTGGCGCTTCTTCCTGGCGGCGGCGGGCTCGCTCGCTATCAGCGGCAGCGCGCTGCCGCTGGt > 1:656369/1‑143 (MQ=255) tccTTCGTTGACGTGCCTTACTGTGCCCTGCCGTGCGTCATCACCGCCGACCCGATAGTGCGTCACGTCCTGCATTCCTGGCGCTTCTGCCTGGCGGCGGCGGGCTCGCTCGCTATCAGCGGTATCGCGCTGCCGCTGGTGAg < 2:124251/142‑1 (MQ=255) acCTTCGTTAACGTGCCGTACTGCGCCTTGCCGGGCGTCGTCCCCGCCGACCCGGAAGAGCGTCACGCCCTGCAGTCCTGGCGCTTCTTCCTGGCGGCGGCGGGCTCGCTCGCTATCAGCGGCATCGCGCTGCCGCTGGTGAg < 1:430009/143‑1 (MQ=255) ccTTCGTTAACGTGCCGTACTGCGCCATGCCGGGCGTCATCACCGCCGACCCGAAAGAGCGTCACGCCCTGCAGTCCTGGCGCTTCTTCCTGGCGGCGGCGGGCTCGCTCGCTATCAGCGGCATCGCGCTGCCGCTGGTGAGc < 1:875087/143‑1 (MQ=255) tAACGTGCCGTACTGCGCCATGCCGGGCGTCATCACCGCCGACCCGAAAGAGCGTCACGCCCTGCAGTCCTGGCGCTTCTTCCTGGCGGCGGCGGGCTCGCTCGCTATCAGCGGCATCGCGCTGCCGCTGGTGAGCATCATCg < 2:220881/143‑1 (MQ=255) ccGTACTGCGCCATGCCGGGCGTCATCACCGCCGACCCGAAAGAGCGTCACGCCCTGCAGTCCTGGCGCTTCTTCCTGGCGGCGGCGGGCTCGCTCGCTATCAGCGGCATCGCGCTGCCGCTGGTGAGCATCATCGGCAAAgg < 1:29548/143‑1 (MQ=255) gTACTGCGCCATGCCGGGCGTCATCACCGCCGACCCGAAAGAGCGTCACGCCCTGCAGTCCTGGCGCTTCTTCCTGGCGGCGGCGGGCTCGCTCGCTCTCAGCGGCATCGCGCTGCCGCTGGTGAGCATCATCGGCAAAgggg > 1:481586/1‑143 (MQ=255) actcggcCATGGCGGGCGTCATCACCGCCGACCCGAACGAGCGTCACGCCCTGCAGTCCTGGCGCTTCTTCCTGGGGGCGGCGGGCTCGCTCGCTATCAGCGGCATCGCGCTGCCGCTGGTGATCATCATCGGCAAAGGGGAc < 2:861521/138‑1 (MQ=255) aCTGCGCCATGCCGGGCGTCATCACCGCCGACCCGAATGAGCGTCACGCCCTGCAGTCCTGGCGCTTCTTCCTGGCGGCGGCGGGCTCGCTCGCTATCAGCGGCACCGCGCTGCCGCTGGTGAGCGTCATCGGCCAAGGGGAc > 2:135602/1‑143 (MQ=255) tGCGCCATGCCGGGCGTCATCACCGCCGACCCGAAAGAGCGTGACGCCCTGCAGTCCTGACGCGGCTTCCTGGCGGCGGCGGGCTCGCTCGCTATCAGCGGCATCGCGCTGCCGCTGGTGAGCATCATCGGCAAAGGGGACGa < 1:331362/143‑1 (MQ=255) tGCGCCATGCCGGGCGTCATCACCGCCGACCCGAAAGAGCGTCACGCCCTGCAGTCCTGGCGCTTCTTCCTGGCGGCGGCGGGCTCGCTCGCTATCAGCGGCATCGCGCTGCCGCTGGTGCGCGTCATCGGCAAAGGGGGCGa > 1:118546/1‑143 (MQ=255) gcgcCATGCCGGGCGTCATCACCGCCGACCCGAAAGAGCGTCACGCCCTGCAGTCCTGGCGCTTCTTCCTGGCGGCGGCGGGCTCGCTCGCTATCAGCGGCATCGCGCTGCCGCTGGTGAGCATCATCGGCCAAGGGGACGAg > 1:547494/1‑143 (MQ=255) ccATGCCGGGCGTCATCACCGCCGACCCGAAAGAGCGTCACGCCCTGCAGTCCTGGCGCTTCTTCCTGGCGGCGGCGGGCTCGCTCGCTATCAGCGGCATCGCGCTGCCGCTGGTGAGCATCATCGGCAAAGGGGACGAGCAg > 1:768705/1‑143 (MQ=255) ccGGGCGTCATCACCGCCGACCCGAAAGAGCGTCACGTCCTGCAGTCCTGGCGCTTCTTCCTGGCGGCGGCGGGCTCGCTCGCTATCAGCGGCATCGCGCTGCCGCTGGTGAGCATCATCGGCAAAGGGGACGAGCAgggggg > 1:496586/1‑143 (MQ=255) ccGGGCGTCATCACCGCCGACCCGAAAGAGCGTCACGCCCTGCAGTCCTGGCGCTTCTTCCTGGCGGCGGCGGGCTCGCTCGCTATCAGCGGCATCGCGCTGCCGCTGGTGAGCATCATCGGCAAAGGGGACGAGCAGGTggg > 1:101917/1‑143 (MQ=255) ccGGGCGTCATCACCGCCGACCCGAAAGAGCGTCACGCCCTGCAGTCCTGGCGCTTCTTCCTGGCGGCGGCGGGCTCGCTCGCTATCAGCGGCATCGCGCTGCCGCTGGTGAGCATCATCGGCAAAGGGGACGAGCAGGTggg < 1:402982/143‑1 (MQ=255) catcaCCGCCGACCCGAAAGAGCGTCACGCCCTGCAGTCCTGGCGCTTCTTCCTGGCGGCGGCGGGCTCGCTCGCTATCAGCGGCCTCGCGCTGCCGCGGGGGAGCGTCGTCGGCAAAGGGGGCGCGCAGGTGGGATGCTTCg > 1:133872/1‑143 (MQ=255) tcaCCGCCGACCCGAAAGAGCGTCACGCCCTGCAGTCCTGGCGCTTCTTCCTGGCGGCGGCGGGCTCGCTCGCTATCAGCGGCATCGCGCTGCCGCTGGTGAGCATCATCGGCAAAGGGGACGAGCAGGTGGGCTACTTCGgc > 1:522043/1‑143 (MQ=255) caCCGCCGACCCGAAAGAGCGTCACGCCCTGCAGTCCTGGCGCTTCTTCCTGGCGGCGGCGGGCTCGCTCGCTATCAGCGGCATCGCGCTGCCGCTGGTGAGCATCATCGGCAGAGGGGACGAGCAGGTGGGCTACTTCGgcg > 1:738581/1‑143 (MQ=255) ggACCCGAACGAGCGCCAGGGCCTGCTGTCCTGGCGCTTCTTCCTGGCGGCGGCGGGCTTGCTCGCTATCAGCGGCATCGCGCGGCCGCTGGTGAGCATCATCGGCAAAGGGGACGAGCAGGTGGGCTACTTCGGCGCCAtgt < 2:850969/142‑1 (MQ=255) aCCCGAAAGAGCGTCACGCCCTGCAGTCCTGGCGCTTCTTCCTGGCGGCGGCGGGCTCGCTCGCTATCAGCGGCATCGCGCTGCCGCTGGTGAGCATCATCGGCAAAGGGGACGAGCAGGTGGGCTACTTCGGCGCCATgtgc < 1:766496/143‑1 (MQ=255) ccGAAAGAGCGTCACGCCCTGCAGTCCTGGCGCTTCTTCCTGGCGGCGGCGGGCTCGCTCGCTATCAGCGGCAGCGCGCTGCCGCTGGGGAGCATCAGCGGCAAAGGGGACGAGCAGGTGGGCCACCTCCGCCCCATgcgcgg > 2:161861/1‑142 (MQ=255) cGAAAGAGCGTCACGCCCTGCAGTCCTGGCGCTTCTTCCTGGCGGCGGCGGGCTCGCTCGCTATCAGCGGCATCGCGCTGCCGCTGGTGAGCATCATCGGCAAAGGGGACGAGCAGGTGGGCTACTTCGGCGCCATgtgcgtg < 1:693538/143‑1 (MQ=255) agagCGTCACGCCCTGCAGTCCTGGCGCTTCTTCCTGGCGGCGGCGGGCTCGCTCGCTATCAGCGGCATCGCGCTGCCGCTGGTGAGCATCATCGGCAAAGGGGACGAGCAGGTGGGCTACTTCGGCGCCATGTGCGTGCTgg > 1:720700/1‑143 (MQ=255) cgCGTCACGCCCTGCAGTCCTGGCGCTTCTTCCTGGCCGCGGCGGGCTCGCTCGCTATCAGCGGCATCGCGCTGCCGCTGGTGAGCATCATCGGCAAAGGGGACGAGCAGGTGGGCTACTTCGGCGCCATGTGCGTGCTgggg < 1:349950/142‑1 (MQ=255) cGTCACGCCCTGCAGTCCTGGCGCTTCTTCCTGGCGGCGGCGGGCTCGCTCGCTATCAGCGGCATCGCGCTGCCGCTGGTGAGCATCATCGGCAAAGGGGACGAGCGGGGGGGCTACTTCGGCGCCATGTGCGTGCTGGGGCt > 1:693995/1‑143 (MQ=255) gTCACGCCCTGCAGTCCTGGCGCTTCTTCCTGGCGGCGGCGGGCTCGCTCGCTATCAGCGGCATCGCGCTGCCGCTGGTGAGCATCATCGGCAAAGGGGACGAGCAGGTGGGCTACTTCGGCGCCATGTGCGTGCTGGAGCTg > 1:619893/1‑143 (MQ=255) cGCCCTGCAGTCCTGGCGCTGCTGCCTGGCGGCGGCGGGCTCGCTCGCTATCAGCGGCATCGCGCTGCCGCTGGTGAGCATCATCGGCAAAGGGGACGAGCAGGTGGGCTACTTCGGCGCCATGTGCGTGCTGGGGCTGAgcg < 2:215760/143‑1 (MQ=255) tGCAGTCCTGGCGCTTCTTCCTGGCGGCGGCGGGCTCGCTCGCTATCAGCGGCATCGCGCTGCCGCTGGTGAGCATCATCGGCAAAGGGGACGAGCAGGTGGGCTACTTCGGCGCCATGCGCGTGCTGGGGCTGAgcggcgcg > 2:209250/1‑141 (MQ=255) ggccggccTGGCGCTTCTTCCTGGCGGCGGCGGGCTCGCTCGCTATCTGCGGCATCGGGCTGCCGCTGGTGAGCATCATCGGCAAAGGGGACGAGCAGGTGGGCTACTTCGGCGCCATGTGCGTGCTGGGGCTGAGCGGCgtg < 2:480296/137‑1 (MQ=255) aGTCCTGGCGCTTCTTCCTGGCGGCGGCGGGCTCGCTCGCTATCAGCGGCATCGCGCTGCCGCTGGTGAGCATCATCGGCAAAGGGGACGAGCAGGTGGGCTACTTCGGCGCCATGTGCGTGCTGGGGCTGAGCGGCgtggtg < 1:309387/143‑1 (MQ=255) tCCTGGCGCTTCTTCCTGGCGGCGGCGGGCTCGCTCGCTATCAGCGGCATCGCGCTGCCGCTGGTGAGCCTCATCGGCAAAGGGGACGAGCAGGTGGGCTACTTCGGCGCCATGTGCGTGCTGGGGCTGAGCGGCGTGGtgct > 1:37141/1‑143 (MQ=255) ccTGGCGCTTCTTCCTGGGGGCGGCGGGCTCGCTCGCTATCAGCGGTACCGCGCTGCCGCTGGTGAGCATCATCGGCAAAGGGGACGAGCAGGTGGGCTACTTCGGCGCCATGTGCGTGCTGGGGCTGAGCGGCGTGGtgctg < 1:678000/143‑1 (MQ=255) ggCGCTTCTGCCTGGCGGCGGCGGGCTCGCCCGCTATCAGCGGCATCGCGCTGCCGCTGGTGAGCATCATCGGCAAAGGGGACGAGCAGGTGGGCTACTGCGGCGCCATGTGCGTGCTGGGGCTGAGCGGCGTGGTGCTGctc < 2:459759/143‑1 (MQ=255) gtgcTTCTTCCTGGCGGCGGCGGGCTCGCTCGCTATCAGCGGCATCGCGCTGCCGCTGGTGAGCATCATCGGCAAAGGGGACGAGCAGGTGGGCTACTTCGGCGCCATGTGCGTGCTGGGGCTGAGCGGCGTGGtgctcccct > 2:462180/3‑138 (MQ=255) cttcttCCTGGCGGCGGCGGGCTCGCTCGCTATCAGCGGCATCGCGCTGCCGCTGGTGAGCATCATCGGCAAAGGGGACGAGCAGGTGGGCTACTTCGGCGCCATGTGCGTGCTGGGGCTGAGCGGCGTGGTGCTGCTCTACg > 1:28024/1‑143 (MQ=255) ttcttcCTGGCGGCGGCGGGCTCGCTCGCTATCAGCGGCATCGCGCTGCCGCTGGTGAGCATCATCGGCAAAGGGGACGAGCAGGTGGGCTACTTCGGCGCCATGTGCGTGCTGGGGCTGAGCGGCGTGGTGCTGCTCTACGt < 1:252455/143‑1 (MQ=255) tcCTGGCGGCGGCGGGCTCGCTCGCTATCAGCGGCATCGCGCTGCCGCTGGTGAGCATCATCGGCAAAGGGGACGAGCAGGTGGGCTACTTCGGCGCCATGTGCGTGCTGGGGCTGCGCGGCGGGTGGCTGCTCTACGTCTGc > 1:235514/1‑143 (MQ=255) tcCTGGCGGCGGCGGGCTCGCTCGCTATCAGCGGCATCGCGCTGCCGCTGGTGAGCATCATCGGCAAAGGGGACGAGCAGGTGGGCTACTTCGGCGCCATGTGCGTGCTGGGGCTGAGCGGCGTGGTGCTGCTCTACGTCCGc > 1:197466/1‑143 (MQ=255) tcCGGGCGGCGGCGGGCTCGCGCGCTATCAGCGGCATCGCGCTGCCGCTGGTGAGCAGCATCGGCAAAGGGGACGAGCAGGTGGGCGACTTCGGCGCCATGTGCGTGCTGGGGCTGAGCGGCGTGGTGCTGCTCTACGTCTGc < 1:207708/143‑1 (MQ=255) ggggggcggcggGCGCGCACGCTGGGAGCGGCACCCCGCTGCCGCGGGTGAGCATCATCGGCAAAGGGGACGCGCATGTGGGCTACTTCGGCGCCATGTTCGTGCTGGGGCTGAGCGGCGTGGTGCTGCTCTACGTCTGcttc < 1:156210/139‑1 (MQ=255) ggcggcggcGGGCTCGCTCGCTATCAGCGGCATCGCGCTGCCGCTGGTGAGCATCATCGGCAAAGGGGACGAGCAGGTGGGCTACTTCGGCGCCATGTGCGTGTGGGGGCTGAGCGGCGTGGTGCTGCGCTACGTCTGcttct > 1:43039/1‑143 (MQ=255) gcggcggGCTCTCTCGCTATCAGCGTCATCGCTCTGCCGCTTGTGAGCATCATCGGCGACGGGGACGAGCAGGTGGGCTACTGCGGCGCCATGTGCGTGCTGGGGCTGAGCGGCGTGGTGCTGCTCTACGTCTGCTTCTTCAc < 2:451581/143‑1 (MQ=255) ggcggGCTCGCTCGCTATCAGCGGCATCGCGCTGCCGCTGGTGAGCATCATCGGCAAAGGGGACGAGCAGGTGGGCTACTTCGGCGCCATGTGCGTGCTGGGGCTGAGCGGCGTGGTGCTGCTCTACGTCTGCTTCTTCACGa < 1:182908/143‑1 (MQ=255) gcggGCTCGCTCGCTATCAGCGGCATCGCGCTGCCGCTGGTGAGCATCATCGGCAAAGGGGACGAGCAGGTGGGCTACTTCGGCGCCATGTGCGTGCTGGGGCTGAGCGGCGTGGTGCTGCTCTACGTCTGCTTCTTCACGAc < 1:854388/143‑1 (MQ=255) cggGCTCGCTCGCTATCAGCGGCATCGCGCTGCCGCTGGTGAGCATCATCGGCAAAGGGGACGAGCAGGTGGGCTACTTCGGCGCCATGTGCGTGCTGGGGCTGAGCGGCGTGGTGCTGCTCTCCGTCTGCTTCGTCACGGcc > 2:522955/1‑143 (MQ=255) gtccgCGGGATCGCGCTGTCGCTGGTGAGCATGAGCGGCAAAGGGGACGAGCAGGTGGGCTACTTCGGCGCCATGTGCGTGCTGGGGCTGAGCGGCGTGGTGCTGCTCTACGTCTGCTTCTTCACGACCAAAGAGCGCTacac < 2:426383/139‑1 (MQ=255) accAGCGGCAGCGCGATGCCGCTGATGAGCATCATCGGCAAAGGGGACGAGCAGGTGGGCTACTTCGGCGCCATGTGCGTGCTGGGGCTGAGCGGCGTGGTGCTGCTCTACGTCTGCTTCTTCACGGCTAAAGAGCGCTacac > 1:181042/3‑143 (MQ=255) aGCGGCATCGCGCGGCCGCGGGTGAGCATCAGCGGCAAAGGGGACGAGCAGGTGGGCTACTTCGGCGCCATGTGCGTGCTGGGGCTGAGCGGCGTGGTGCTGCTCTACGTCTGCTGCTTCACGACCAAAGAGCGCTACACCtt < 1:144893/143‑1 (MQ=255) aGCGGCAGCGCGATGCCGCTGGTGAGCATCATCGGCAAAGGGGACGAGCAGGTGGGCTACTTCGGCGACATGTGCGTGCTGGGGCTGAGCGGCGTGGTGCTGCTCTACGTCTGCTTCTTCACGACCAAAGAGCGCTACACCtt > 1:698373/1‑143 (MQ=255) gCATCGCGCTGCCGCTGGTGAGCATCATCGGCAAAGGGGACGAGCAGGTGGGCTACTTCGCCGCCATGTGCGTGCTGGGGCTGAGCGGCGTGGTGCTGCTCTACGTCTGCTTCTTCACGACCAAAGAGCGCTACACCTTTGAg < 2:311191/143‑1 (MQ=255) aTCGCGCTGCCGCTGGTGAGCATCATCGGCAAAGGGGACGAGCAGGTGGGCTACTTCGGCGCCATGTGCGTGCTGGGGCTGAGCGGCGTGGTGCTGCTCTACGTCTGCTTCTTCACGACCAAAGAGCGCTACACCTTTGAGGt > 1:470339/1‑143 (MQ=255) tCGCGCTGCCGCTGGTGAGCATCATCGGCAAAGGGGACGAGCAGGTGGGCTACTTCGGCGCCATGTGCGTGCTGGGGCTGAGCGGCGTGGTGCTGCTCTACGTCTGCTTCTTCACGACCAAAGAGCGCTACACCTTTGAGGTg < 1:255923/143‑1 (MQ=255) tCGCGCTGCCGCTGGTGAGCATCATCGGCAAAGGGGACGAGCAGGTGGGCTACTTCGGCGCCATGTGCGTGCTGGGGCTGAGCGGCGTCGTGATGACCAGCACCCATGTCTTCAAGACCGAAGAGCGCTACACCTTTGAGGTg > 1:647002/1‑143 (MQ=255) | CCTGGTCTACACCTTCGTTAACGTGCCGTACTGCGCCATGCCGGGCGTCATCACCGCCGACCCGAAAGAGCGTCACGCCCTGCAGTCCTGGCGCTTCTTCCTGGCGGCGGCGGGCTCGCTCGCTATCAGCGGCATCGCGCTGCCGCTGGTGAGCATCATCGGCAAAGGGGACGAGCAGGTGGGCTACTTCGGCGCCATGTGCGTGCTGGGGCTGAGCGGCGTGGTGCTGCTCTACGTCTGCTTCTTCACGACCAAAGAGCGCTACACCTTTGAGGTG > NC_000913/285748‑286024 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |