| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 3,560,455 | +G | pseudogene (151/758 nt) | glpR ← | DNA‑binding transcriptional repressor GlpR |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 3,560,455 | 1 | . | G | 96.1% | 151.2 / ‑1.1 | 51 | pseudogene (151/758 nt) | glpR | DNA‑binding transcriptional repressor GlpR |
| Reads supporting (aligned to +/- strand): ref base . (1/1); new base G (20/29); total (21/30) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.61e-01 | |||||||||||
GATAAACAGCGTCGAGCCATTGGGGATTTGCTCCGCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCAT‑GATGG‑C‑CAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCGATAATACCGTTGTG > NC_000913/3560316‑3560587 | gATAAACAGCGTCGAGCCATTGGGGATTTGCTCCGCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGGACCGCCAT‑GATGG‑cgcc > 2:102778/1‑140 (MQ=255)gATAAACAGCGTCGAGCCATTGGGGATTTGCTCCGCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCAC‑GATGG‑cgaa > 2:141324/1‑140 (MQ=255) aCAGCGTCGAGCCATTGGGGATTTGCTCCGCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCAT‑GATGG‑CGCAGGATc < 1:765827/143‑1 (MQ=255) tCGAGCCATTGGGGATTTGCTCCGCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCAT‑GATGG‑CGCAGGATCAGGttt < 1:103259/143‑1 (MQ=255) cATTGGGGATTTGCTCCGCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCAT‑GATGT‑CGCTGTATCAGGTTTTGCTcc > 1:199561/1‑143 (MQ=255) ggtgggttGCGCCGCCACCGCGCGGGCTATGCGCTCCTGCTCTCCGGGCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCAT‑GATGG‑CGCAGGATCAGGTTTTGCTCCGCCa < 2:645430/137‑1 (MQ=255) ggggATTTGCTCCGCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCAT‑GATGG‑CGCCGGATCCGGTGTTGCTCCGCCa > 1:1196859/1‑143 (MQ=255) ggctttGGTCTGCTACTTGGCGGGCGGTGCGCTCTTTTTCTTGGGTCTGTGTGGCCTTGCGATCGTGCGACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCAT‑GATGG‑CGCAGGATCAGGTTTTGCTCCGCCAGc < 2:633413/140‑1 (MQ=255) ggATTTGCTCGGTCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGCGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCAT‑GATGG‑CGCAGGATCAGGTTTTGCTCCGCCAGc < 2:59033/143‑1 (MQ=255) gATTTGCTCCGCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGCGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCAT‑GATGG‑CGCAAGATCAGGTTGTGACCCGCCAGCt > 2:139874/1‑143 (MQ=255) tcgcGCCAGTTGGGGGGCGCAGCGCCCTTTTTCTTGGTTCTGGGTGGCCTTGCGAGCGTGCCCCGGCGTGTTAGCCGAACTGGAAGGCAGCGCCGCACCGCCAT‑GATGG‑CGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGa < 2:553043/140‑1 (MQ=255) tCCGCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGACCTGGATGGCATCGCCGCACCGCCAT‑GATGG‑CGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAg < 1:1079224/143‑1 (MQ=255) tCCGCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCAT‑GATGG‑CGCAGGATCAGGTTTTGCTCCGCCCGCTCATTgcg > 1:359070/1‑141 (MQ=255) tCCGCCACTTTGCGGGCGATGCGCTCTTTTTATTACGTCTATTTAGCCTTGCGATCGTGCCACGGCGTGTTAACCGATCGGGAGTGCGGCGCCGCACCGCCAT‑GATGG‑CGCAGGATCAGGTGTTGCTCCGCCAGCTCATTGAg > 2:144048/1‑143 (MQ=255) ctgtGCGGGCGCTGCGCTCTTCGTCTTCTGTCTGGGTGGCTTCGCGGTCGTGCCACGGCGTGTTACCCGACCTGGAAGGCAGCGCCGCACCGTCAT‑GATGG‑CGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCgcgg < 2:456252/140‑1 (MQ=255) cTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCAT‑GATGG‑CGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCgcgg < 1:469850/143‑1 (MQ=255) tgtcggggATGCGCTCGTTGTCTTCGGTCTTGGTGGCCTTGCGATCGTGCCACGGCGTGTGCACCGAACTGGAAGGCAGCGCCGCACCGCCAT‑GATGG‑TGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGa < 2:962939/136‑1 (MQ=255) tGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCAT‑GATGG‑CGCAGGATCAGGTCTTGCGCCGCCACCTCATTGATGACGCGGCGa > 2:885424/1‑143 (MQ=255) gCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCAT‑GATGG‑CGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGaa > 1:889060/1‑143 (MQ=255) gggCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGGCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCAT‑GATGG‑CGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATa < 2:470823/143‑1 (MQ=255) ggCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCAT‑GATGC‑CGCAGGATCAGGTTTTGCTCCGCCAGCGCATTGAGGGCGCGGCGACTAg > 1:97808/1‑143 (MQ=255) gcgcTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCAT‑GATGG‑CGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCg < 2:581218/143‑1 (MQ=255) ctctTGTTCTTCGGCCAGTGTGTCCGTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCAT‑GATGG‑CGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGc < 1:315572/143‑1 (MQ=255) ttcGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCAT‑GATGG‑CGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGCCGCGGCGACTAGTATGCGGGCTGCCGggga > 2:1152817/1‑143 (MQ=255) ttcGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCAT‑GATGG‑CGCAGGATCAGCTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGgaga > 1:613095/1‑143 (MQ=255) ggTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCAT‑GATGG‑CGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAAt < 1:561477/143‑1 (MQ=255) gCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCAT‑GATGG‑CGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAg < 1:1093340/143‑1 (MQ=255) ttGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCAT‑GATGG‑CGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGctc > 2:230575/1‑143 (MQ=255) ttGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCAT‑GATGG‑CGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGctc < 2:513907/143‑1 (MQ=255) gCGATCGTGCCACGGCGTGTTAACCGAACTGGAGGGCAGCGCCGCACCGCCAT‑GATGG‑CGCAGGATCAGGGTGTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAAGGCTCTACCAGCTCtt < 2:54702/143‑1 (MQ=255) gATCCTGCCAGGGTGTGTTAACCGAAGTGGAAGGCAGCGCCGCACCGCCAT‑GATGG‑CGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCg < 1:1046471/143‑1 (MQ=255) cGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCAT‑GATGG‑CGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTa < 1:703930/143‑1 (MQ=255) gCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCAGCACAGCCAT‑GATGGAG‑CAGGATCAGGTTTTGCTACGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCCCTACCAGCTCTTCggcaatg > 1:1114967/1‑138 (MQ=255) cgcGGCGTGTTGACCGAACTGGAAGGCGACGCCGCGCGGCCAT‑GATGG‑CGCAGGATCAGGTTTTGCTCCGCCAGCTCAGTGAGGTCGCGGCGACTAGTCTGCGGGATGTCGTGGCAATGCTCTACCAGCTCTTCGGTACTGAc < 2:88924/141‑1 (MQ=255) gctgggtAACCGAGCTGGAAGGCAGCGCCGTCGTGCGATACACAC‑C‑CAGGAGCGGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGTAGAAATGCTCTACCAGCTCTTCGGTACTGACATaa < 2:247026/137‑1 (MQ=255) gtgtTAACCGAACTGGAAGGCAGCGCCGCACCGCCAT‑GATGG‑CGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAAcc < 1:1073780/143‑1 (MQ=255) gtgtTAACCGAACTGGAAGGCAGCGCCGCACCGCCAT‑GATGG‑CGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAAcc < 1:575139/143‑1 (MQ=255) gtgtGAGCCAAACGGGGAGGCAGCGCCGCACCGCCAT‑GATGG‑CGCAGGATCAGGTTTTGCTCCGCCAGCTCAGTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTGCCAGCTCTTCGGTACTGACATAAcc < 2:263949/143‑1 (MQ=255) aaCCGAACTGGAAGGCAGCGCCGCACCGCCAT‑GATGG‑CGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCctgct > 1:583878/1‑143 (MQ=255) aaCTGGAAGGCAGCGCCGCACCGCCAT‑GATGG‑CGCAGGATCAGGTGTTGCTCCGCCAGCTCATTGCGGTCGCGGCGAATCGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCCCTTCGGTACTGACATCACCCTGCTGgttc > 1:417615/1‑142 (MQ=255) cTGCAAGGCAGCGCCGCACCGCCGT‑GGTGG‑CGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAAc < 2:1200991/143‑1 (MQ=255) tGGAAGGCAGCGCCGCACCGCCAT‑GATGG‑CGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAAcc > 1:682913/1‑143 (MQ=255) gcaaGGCAGCGACGCACCGCCAT‑GATGG‑CGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCa < 1:612368/141‑1 (MQ=255) gAAGGCAGCGCCGCACCGCCAT‑GATGG‑CGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGTGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAAACAg > 1:606199/1‑143 (MQ=255) cggCAGCGCCGCACCGCCAT‑GATGG‑CGCAGTATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGtt < 1:290306/142‑1 (MQ=255) cAGCGCCGCACCGCCAT‑GATGG‑CGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGGGAAGAGTCTGCGGGCTTACGGAGAAATGATCTGCCAGCGCTTAGGTCATGACAGAAGCCCGCTGGTTAGCCAGTTcta > 2:28780/1‑141 (MQ=255) cACCGCCAT‑GATGG‑CGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCCGCTGTTCAACCAGTTCGACAATACCg > 2:1157352/1‑143 (MQ=255) aCCGCCAT‑GATGG‑CGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCGATAATACCGt < 1:720436/143‑1 (MQ=255) ccGCCAT‑GATGG‑CGCAGGACCAGGTGTTGCGCCGCCAGCTCAGTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCGATAATACCGtt < 1:586079/143‑1 (MQ=255) cGCCAT‑GATGG‑CGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTAGTGACATAACCCTGCTGGTTAACCAGTTCGATAATACCGtgg > 1:1073244/1‑141 (MQ=255) cGCCAT‑GATGG‑CGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCGATAATACCGTtg > 1:1022497/1‑143 (MQ=255) gCCAT‑GATGG‑CGCAGGATCAGGTTTTGCTCCGCCAACACCTTGAGATCGCGGAGAATAGTCTGCGGGCTGAGGGAGAATTACGTTAGCGGCTACTCGGTACTGTCATAACCCTGGTGTTTAAGCAGTTCGGTAATCCCGTtgt > 1:444194/1‑143 (MQ=255) ccAT‑GATGG‑CGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCGATAATACCGTtgtg < 1:897205/143‑1 (MQ=255) | GATAAACAGCGTCGAGCCATTGGGGATTTGCTCCGCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCAT‑GATGG‑C‑CAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCGATAATACCGTTGTG > NC_000913/3560316‑3560587 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |