| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 3,637,646 | +T | coding (5/1500 nt) | pitA → | metal phosphate:H(+) symporter PitA |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 3,637,645 | 1 | . | T | 90.4% | 151.9 / 8.2 | 53 | coding (4/1500 nt) | pitA | metal phosphate:H(+) symporter PitA |
| Reads supporting (aligned to +/- strand): ref base . (2/3); new base T (21/26); total (24/29) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
TATATAAGATAATTATTTTTTGAGTGAAATCCATACAGGGGGCAAATCAAAAAAAGTCTATATTTCACTTTGCCCGCGCCGCGAAAGTCACTGATAATGCGCCGCGTTCATGTCCTCAAAATGGCGTAACGTCCTATG‑C‑T‑ACATTTGTTTGCTGGCCTGGATTTGCATACCGGGCTGTTATTATTGCTTGCACTGGCTTTTGTGCTGTTCTACGAAGCCATCAATGGTTTCCATGACACAGCCAACGCCGTGGCAACCGTTATCTATACCCGCGC > NC_000913/3637507‑3637781 | tatataAGAGAATTATTTTTTGAGTGAAATCCATACAGGGGGCAAATCAAAAAAAGTCTATATTTCACTTTGCCCGCGCCGCGAAAGTCACTGATAATGCGCCGCGTTCATGTCCTCAAAATGGCGTAACGTCCTATG‑C‑ttac < 1:343757/143‑4 (MQ=255) atataAGATAATTATTTTTTGAGTGAAATCCATACAGGGGGCAAATCAAAAAAAGTCTATATTTCACTTTGCCCGCGCCGCGAAAGACACTGATAATGCGCCGCGTTCATGCCCTCAAAGTGGCGTAACGCGCTATG‑cgtaca > 2:978074/1‑138 (MQ=255) taAGTTAATTAGATTGTGAGGGAAATCCATACAGGGGGCAAAACAAAAAAAGTCTATATTTCACTTTGCCCGCGCCGCGAAAGTCACTGATAATGCGCCGCGTTCATGTCCTCAAAATGGCGTAACGTCCTATG‑CTT‑ACAttt < 2:773277/143‑1 (MQ=255) taAGATAATTATTTTTTGAGTGAAATCCATACAGGGGGCAAATCAAAAAAAGTCTATATTTCACTTTGCCCGCGCCGCGAAAGTCACTGATAATGCGCCGCGTTCATGTCCTCAAAATGGCGTAACGTCCTATG‑C‑TGACAttt > 1:534466/1‑143 (MQ=255) aaTCATTTTTTGAGTGAAATCCATACAGGGGGCAAATCAAAAAAAGTCTATATTTCACTTTGCCCGCGCCGCGAAAGTCACTGATAATGCGCCGCGTTCATGTCCTCAAAATGGCGTAACGTCCTATG‑CTT‑ACATTTGTTTGc < 2:124517/143‑1 (MQ=255) ttattTTTTGAGTGAAATCCATACAGGGGGCAAATCAAAAAAAGTCTATATTTCACTTTGCCCGCGCCGCGAAAGTCACTGATAATGCGCCGCGTTCATGTCCTCAAAATGGCGTAACGTCCTATG‑CTT‑ACATTTGTTTGCTg < 2:273567/143‑1 (MQ=255) ttattTTGTGAGTGAAATCCATACAGGGGGCAAATCAAAAAAAGTCTATATTTCACTTTGCCCGCGCCGCGAAAGTCACTGATAATGCGCCGCGTTCATGTCCTCAAAATGGCGTAACGTCCTATG‑CTT‑ACATTTGTTTGCTg < 1:917049/143‑1 (MQ=255) attTTTTGAGTGAAATCCATACAGGGGGCAAATCAAAAAAAGTCTATCTTTCACTTTGCCCGCGCCGCGAAAGTCACTGATAATGCGCCGCGTTCATGTCCTCAAAATGGCGTAACGTCCTATG‑CTT‑ACATTTGTTTGCTGGc > 1:1149881/1‑143 (MQ=255) tttGAGTGAAATCCATACAGGGGGCAAATCAAAAAAAGTCTATATTTCACTTTACCCGCGCCGCGAAAGTCACTGATAATACGCCACGTTCATGTCCACAAAATGGCGTAACGTCCTATG‑CTT‑ACATCAGTTTGCTGGCGTgg > 1:140677/1‑143 (MQ=255) gAGTGAAATCCATACAGGGGGCAAATC‑AAAAAAGTCTATATTTCACTTTGCCCGCGCCGCGAAAGTCACTGATAATGCGCCGCGTTCATGTCCTCAAAATGGCGTAACGTCCTATG‑CTT‑ACATTTGTTTGCTGGCCTGGAttt < 1:950627/143‑1 (MQ=255) aggcaactCCACACGGGGGGCAAATCAAAACAAGTCTATATTTCACTTTGCCCGCGCCGCGAAAGTCACTGATAATGCGCCGCGTTCATGTCCTCAAAATGGCGTAACGTCCTATG‑CTT‑ACATTTGTTTGCTGGCCTGGAttt < 2:754698/136‑1 (MQ=255) aGTGAAATCCATACAGGGGGCAAATCAAAAAAAGTCTATATTTCACTTTGCCCGCGCCGCGAAAGTCACTGATAATGCGCCGCGTTCATGTCCTCAAAATGGCGTAACGTCCTATG‑CTT‑ACATTTGTTTGCTGGCCTGGAttt < 1:1049981/143‑1 (MQ=255) gTGAAAGCCATACAGGGGGCAAATCAAAAAAAGTCTATATTTCACTTTGCCCGCGCCGCGAAAGTCACTGATAATGCGCCGCGTTCATGTCCTCAAAATGGCGTAACGTCCTATG‑CTT‑ACATTTGTTTGCTGGCCTGGATTTg < 2:672051/143‑1 (MQ=255) tGAAATCCATACAGGGGGCAAATCAAAAAAAGTCTATATTTCACTTTGCCCGCGCCGCGAAAGTCACTGATAATGCGCCGCGTTCATGTCCTCAAAATGGCGTAACGTCCTATG‑CTT‑ACATTTGTTTGCTGGCCTGGATTTGc > 1:851855/1‑143 (MQ=255) gcgaaTCCATACAGGGGGCAAATCAAAAAAAGTCTATATTTCACGTTGCCCGCGCCGCGAAGGTCACTGATAATGCGCCGCGTTCATGTCCTCAAAATGGCGTAACGTCCTATG‑CTT‑ACATTTGTTTGCTGGCCTGGATTTGc < 1:485465/140‑1 (MQ=255) ttCCATACAGGTGGCAAATCAAAAAAAGTCTATATTTCACTTTGCCCGCGCCGCGAAAGTCACTGATAATGCGCCGCGTTCATGTCCTCAAAATGGCGTAACGTCCTATG‑CTT‑ACATTTGTTTGCTGGCCTGGATTTGCATAc < 1:311778/142‑1 (MQ=255) tACAGGGGGCAAATCAAAAAAAGTCTATATTTCACTTTGCCCGCGCCGCGAAAGTCACTGATAATGCGCCGCGTTCATGTCCTCAAAATGGCGTAACGTCCTATG‑CTT‑ACATTTGTTTGCTGGCCTGGATTTGCATACCGGGc > 1:287045/1‑143 (MQ=255) tACAGGGGGCAAATCAAAAAAAGTCTATATTTCACTTTGCCCGCGCCGCGAAAGTCACTGATAATGCGCCGCATTCATGTCCTCAAAATGGCGTAACGTCCTATG‑CTT‑ACATTTGTTCGCTGGCATGGGTTTGCACACCGGGc > 2:957697/1‑143 (MQ=255) gggggCAAATCAAAAAAAGTCTATATTTCACTTTGCCCGCGCCGCGAAAGTCACTGATAATGCGCCGCGTTCATGTCCTCAAAATGGCGTAACGTCCTATG‑CTT‑ACATTTGTTTGCTGGCCTGGATTTGCATACCGGGCTGtt < 1:862497/143‑1 (MQ=255) ggCAAATCAAAAAAAGTCGATATTTCACTTTGCCCGCGCCGCGAGAGTCACTGATAATGCGCCGCGTTCATGTCCTCGAGATGGCGTAACGTCCTATG‑CTT‑ACATTTGTTTGCTGGCCTGGATTTGCATACCGGGCTGttatt < 2:971984/143‑1 (MQ=255) aTCAAAAAAAGTCTATATTTCACTTTGCCCGCGCCGCGAAAGTCACTGATAATGCGCCGCGTTCATGTCCTCAAAATGGAGTAACGTCCTATG‑CTT‑ACATTTGTTTGCTGGCCTGGATTTGCATACCGGGCTGTTATTAttgc < 1:979125/143‑1 (MQ=255) aaaaaaGTCTATATTTCACTTTGCCCGCGCCGCGAAAGTCACTGATAATGCGCCGCGTTCATGTCCTCAAAATGGCGTAACGTCCTATG‑CTT‑ACATTTGTTTGCTGGCCTGGATTTGCATACCGGGCTGTTATTAttgcttgc > 1:760294/1‑143 (MQ=255) aaaaaGTCTATATTTCACTTTGCCCGCGCCGCGAAAGTCACTGATAATGCGCCGCGTTCATGTCCTCAAAATGGCGTAACGTCCTATG‑CTT‑ACATTTGTTTGCTGGCCTGGATTTGCATACCGGGCTGTTATTATTGCTTGCa < 1:733432/143‑1 (MQ=255) agttCACTTTGCCCGCGCCGCGAACGTCACTGATAATGCGCCGCGTTCATGTCCTCAAAATGGCGTAACGTCCTATG‑CTT‑ACATTTGTTTGCTGGCCTGGATTTGCATACCGGGCTGTTATTATTGCTTGCCCTGGCTTTtgt < 2:266450/141‑1 (MQ=255) tttCACTTTGCCCGCGCCGCGAAAGTCACTGATAATGCGCCGCGTTCATGTCCTCAAAATGGCGTAACGTCCTATG‑CTT‑ACATTTGTTTGCTGGCCTGGATTTGCATACCGGGCTGTTATTATTGCTTGCACTGGCTTTtgtg < 1:1085790/143‑1 (MQ=255) ttCACTTTGCCCGCGCCGCGAAAGTCACTGTTAGTGCGCCGCTGACATGTCCTCAAAATGGCGTAACGTCCTATG‑C‑TAACAGTCGTACGCCAGCCTCAATTGTCATACCGGGCTCTTAGTATTCCCTGCACTTCCTTTTCTGc > 2:305208/1‑143 (MQ=255) cTTTGCCCGCGCCGCGAAAGTCACTGATAATGCGCCGCGTTCATGTCCTCAAAATGGCGTAACGGCCTATG‑CGT‑ACATTTGTTTGCTGGCCTGGATTTGCATACCGGGCTGTTATTATTGCTTGCACTGGCTTGTGTGCTGtt > 1:563631/1‑143 (MQ=255) cccGCGCCGCGAAGGTCACTGATAATGCGCCGCGTTCATGTCCTCAAAATGGCGTAACGTCCTATG‑CTT‑ACATTTGTTTGCTGGCCTGGATTTGCATACCGGGCTGTTATTATTGCTTGCACTGGCTTTTGTGCTGTTCTACg < 1:204380/143‑1 (MQ=255) cgcgccgcgAAAGTCACTGATAATGCGCCGCGTTCATGTCCTCAAAATGGCGTAACGTCCTATG‑CTT‑ACATTTGTTTGCTGGCCTGGATTTGCATACCGGGCTGTTATTATTGCTTGCACTGGCTTTTGTGCTGTTCTACGaa < 1:64516/143‑1 (MQ=255) cgcgAAAGTCACTGATAATGCGCCGCGTTCATGTCCTCAAAATGGCGTAACGTCCTATG‑CTT‑ACATTTGGTTGCTGGCCTGGATTTGCATACCGGGCTGTTTTTATTGCTTGCACTGGCTTTTGTGCTGTGGTACGGAGCCag > 2:985052/1‑142 (MQ=255) tgctactGCGCTGTGTTTAGGTCCGCCAACTGGCGTAACGTCCTATG‑C‑TGACATTTGTTTGCTGGCCTGGATTTGCATACCGGGCTGTTATTATTGCTTGCACTGGCTTTTGTGCTGTTCTACGAAGCCATCAATGGTTTCCa < 2:145406/137‑1 (MQ=255) tGATAATGCGCCGCGTTCATGTCCTCAAAATGGCGTAACGTCCTATG‑CTT‑ACATTTGTTTGCTGGCCTGGATTTGCATCCCGGGCTGTTATTATTGCTTGCACTGGCTTTTGTGCTGTTCTACGAAGCCATCAATGGTTTCCa < 1:465332/143‑1 (MQ=255) tGATAATGCGCCGCGTTCATGTCCTCAAAATGGCGTAACGTCCTATG‑CTT‑ACATTTGTTTGCTGGCCTGGATTTGCATACCGGGCTGTTATTATTGCTTGCACTGGCTTTTGTGCTGTTCTACGAAGCCATCAATGGTTGCCa < 1:116140/143‑1 (MQ=255) gATAATGCGCCGCGTTCATGTCCTCAAAATGGCGTAACGTCCTATG‑CTT‑ACATTTGTGTGCTGGCCTGGATTTGCATACCGGGCTGTTATTATTGCTTGCACTGGCTTTTGTGCTGTTCTACGAAGCCATCAATGGTTTCCAt < 2:376662/143‑1 (MQ=255) cgcgTTCATGTCCTCAAAATGGCGTAACGTCCTATG‑CTT‑ACATTTGTTTGCTGGCCTGGATTTGCATACCGGGCTGTTATTATTGCTTGCACTGGCTTTTGTGCTGTTCTACGAAGCCATCAATGGTTTCCATGACACAGCCa > 2:509266/1‑143 (MQ=255) cgcgTTCATGTCCTCAAAATGGCGTAACGTCCTATG‑CTT‑ACATTTGTTTGCTGGCCTGGATTTGCATACCGGGCGGTTATTATTGCTTGCACTGGCTTTTGTGCTGTTCTACGAAGCCATCAATGGGTTCCATGACACAGccc > 2:650391/1‑142 (MQ=255) tGTCCTCCACATCGCGTAACGTGCTATGCC‑T‑CCATTTGTGTGCGGGCCTGGATTCGCATACCGGGCTGTTATTATTCCTTGCACTGGCTTTTGTGCTGTTCTACGAAGCCATCAATGGTTTCCATGACACAGCCAACGCCGTg < 1:441615/143‑1 (MQ=255) tGTCCTCAAAATGGCGTAACGTCCTATG‑CTT‑ACATTTGTTTGCTGGCCTGGATTTGCATACCGGGCTGTTATTATTGCTTGCACTGGCTTTTGTGCTGTTCTACGAAGCCATCAATGGTTTCCATGACACAGCCAACGCCGTg < 2:534466/143‑1 (MQ=255) tGTCCTCAAAATGGCGTAACGTCCTATG‑CTT‑ACATTTGTTTGCTGGCCTGGATGTGCATACCGGGCTGTTATTATTGCTTGCACTGGCTTTTGTGCTGTTCTACGAAGCCATCAATGGTTTCCATGACACAGCCAACGCCGTg < 2:828670/143‑1 (MQ=255) tCCTCAAAATGGCGTAACGTCCTATG‑CTT‑ACATTTGTTTGCTGGCCTGGATTTGCATACCGGGCTGTTATTATTGCTTGCACTGGCTTTTGTGCTGTTCTACGACGCCATCAATGGTTTCCCTGACACAGCCACCGCCGTGGc > 1:298687/1‑143 (MQ=255) cTCAAAATGGCGTAACGTCCTATG‑CTT‑ACATTTGTTTGCTGGCCTGGATTTGCATACCGGGCTGTTATTATTGCTTGCACTGGCTTTTGTGCTGTTCTACGAAGCCATCAATGGTTTCCATGACACAGCCAACGCCGTGGCaa < 1:1105655/143‑1 (MQ=255) cTCAAAATGGCGTAACGTCCTATG‑CTT‑ACATTTGTTTGCTGGCCTGGATTTGCATACCGGGCTGTTATTATTGCTTGCACTGGCTTTTGTGCTGTTCTACGAAGCCATCAATGGTTTACATGGGACGGCAAAAGCCGTGGCaa > 1:511425/1‑143 (MQ=255) cTCAAAATGGCGTAACGTCCTATG‑CTT‑ACATTTGTTTGCTGGCCTGGATTTGCATACCGGAACATTATTATTGTTTGCACTGGCTTTTGTGCTGTTCTATGTCGCCATCATTGGTTTCCTTGCAACCGCCATCCACGTGGctc > 2:479086/1‑141 (MQ=255) tCAAAATGGCGTAACGTCCTATG‑CTT‑ACATTTGTTTGCTGGCCTGGATTTGCATACCGGGCTGTTATTATTGCTTGCACTGGCTTTTGTGCTGTTCTACGAAGCCATCAATGGCTTCCATGACACAGCCAACGCCGTGGCAAc > 1:463484/1‑143 (MQ=255) aaaaTGGCGTAACGTCCTATG‑CTT‑ACATTTGTTTGCTGGCCTGGATTTGCATACCGGGCTGTTATTATTGCTTGAACTGGCTTTTGTGCTGTTCTACGAAGCCATCAATGGTTTCCATGACACAGCCAACGCCGTGGCAACCg > 1:629725/1‑143 (MQ=255) aTGGCGTAACGTCCTATT‑CTT‑ACATTTGTTTGCTGGCCTGGATTTGCATACCGGGCTGTTATTATTGCTTGCACTGGCTTTTGTGCTGTTCTACGAAGCCATCAATGGTTTCCATGACACAGCCAACGCCGTGGCAACCGTTa < 1:293754/143‑1 (MQ=255) tGGCGTAACTGCCTAGG‑C‑TGACATTTGTTTGCTGGCCTGGATTTGCATACCGGGCTGTTATTATTGCTTTCACTGGATTTGGTTCTGTTCTACGAAGCCATCAATGGTTTCCATGACACAGCCAACGCCGTGGCAACCGTTAt < 2:1111297/143‑1 (MQ=255) tGGCGTAACGTCCTATG‑CTT‑ACATTTGTTTGCTGGCCTGGATTTGCATACCGGGCTGGTATTATTGCTTGCACTAGCTTTTGTGCTGTTCTACGAAGCCATCAATGGTTTCCATGACACAGCCACCGCCGTGGCAACCGTTAt > 1:427446/1‑143 (MQ=255) gCGTAACGTCCTATG‑CTT‑ACATTTGTGTGCTGGCCTGGATTTGCATACCGGGCTGTTATTATTGCTTGCACTGGCTTTTGTGCTGTTCTACGAAGCCATCAATGGTTTCCATGACACAGCCAACGCCGTGGCAACCGTTATCt < 1:911989/143‑1 (MQ=255) gTAACGTCCTATG‑CTT‑ACATTTGTTTGCTGGCCTGGATTTGCATACCGGGCTGTTATTATTGCTTGCACTGGCTTTTGTGCTGTTCTACGAAGCCATCAATGGTTTCCCTGACCCAGCCATCGCCGTGGCAACCGTTGTCtat > 1:786448/1‑143 (MQ=255) tAACGTCCTATG‑CTT‑ACATTTGTTTGCTGGCCTGGATTTGCATACCGGGCTGTTATTATTGCTTGCACTGGCTTTTGTGCTGTTCTACGAAGCCATCAATGGTTTCCATGACACAGCCAACCCCGTGGCCACCGTTATCcata > 1:789494/1‑143 (MQ=255) aaCGTCCTATG‑CTT‑ACATTTGTTTGCTGGCCTGGATTTGCATACCGGGCTGTTATTATTGCTTGCACTGGCTTTTGTGCTGTTATACGCAGCCACCAATGGTTTCGATGTCACAGCTGACGGCGTGGCCACCGTTACCTATAc > 2:27073/1‑143 (MQ=255) gTCCTATG‑CTT‑ACATTTGTTTGCTGGCCTGGATTTGCATACCGGGCTGTTATTATTGCTTGCACTGGCTTTTGTGCTGTTCTACGAAGCCATCAATGGTTTCCATCGCACAGCCAACACCCTGACACCCGCTATCTCTACCcg > 2:610521/1‑143 (MQ=255) cTATG‑CTT‑ACATTTGTTTGCTGGCCTGGATTTGCATACCGGGCTGTTATTATTGCTTGCACTGGCTTTTGTGCTGTTCTACGAAGCCATCAATGGTTTCCATGACACAGCCACCGCCGTGGCAACCGTTTTCTATATCcgcgg > 1:529169/1‑142 (MQ=255) cTATG‑CTT‑ACATTTGTTTGCTGGACTGGATTTGCATACCGGGCTGTTATTATTGCTTGCACTGGCTTTTGTGCTGTTCTACGAAGCCATCAATGGTTTCCATGACCCAGCCAACGCCGTGGCAACCCTTAACTATACCcgcga > 2:270005/1‑142 (MQ=255) | TATATAAGATAATTATTTTTTGAGTGAAATCCATACAGGGGGCAAATCAAAAAAAGTCTATATTTCACTTTGCCCGCGCCGCGAAAGTCACTGATAATGCGCCGCGTTCATGTCCTCAAAATGGCGTAACGTCCTATG‑C‑T‑ACATTTGTTTGCTGGCCTGGATTTGCATACCGGGCTGTTATTATTGCTTGCACTGGCTTTTGTGCTGTTCTACGAAGCCATCAATGGTTTCCATGACACAGCCAACGCCGTGGCAACCGTTATCTATACCCGCGC > NC_000913/3637507‑3637781 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |