| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 125,387 | C→T | Q791* (CAG→TAG) | aceE → | pyruvate dehydrogenase E1 component |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 125,387 | 0 | C | T | 95.5% | 120.8 / NA | 45 | Q791* (CAG→TAG) | aceE | pyruvate dehydrogenase E1 component |
| Reads supporting (aligned to +/- strand): ref base C (1/1); new base T (15/27); total (17/28) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
AGATCCTGGCGAAAGATTACGGCGTAGGTTCTGACGTTTATAGCGTGACCTCCTTCACCGAGCTGGCGCGTGATGGTCAGGATTGTGAACGCTGGAACATGCTGCACCCGCTGGAAACTCCGCGCGTTCCGTATATCGCTCAGGTGATGAACGACGCTCCGGCAGTGGCATCTACCGACTATATGAAACTGTTCGCTGAGCAGGTCCGTACTTACGTACCGGCTGACGACTACCGCGTACTGGGTACTGATGGCTTCGGTCGTTCCGACAGCCGTGAGA > NC_000913/125247‑125525 | agaTCCTGGCGAAAGATTACGGCGTAGGTTCTGACGTTTATAGCGTGACCTCCTTCACCGAGCTGGCGCGTGATGGTCAGGATTGTGAACGCTGGAACATGCTGCACCCGCTGGAAACTCCGCGCGTTCCGTATATCGCTCAg > 2:905407/1‑143 (MQ=255) ccTGGCGAAAGGTTACGGCGTAGGTTCTGACGTTTATAGCGTGACCTCCTTCACCGAGCTGGCGCGTGATGGTCAGGATTGTGAACGCTGGAACATGCTGCACCCGCTGGAAACTCCGCGCGTTCCGTATATCGCTTAGGtga < 2:1033413/143‑1 (MQ=255) ccTGGCGAAAGATTACGGCGTAGGTTCTGACGTTTATAGCGTGACCTCCTTCACCGAGCTGGCGCGTGATGGTCAGGATTGTGAACGCTGGAACATGCTGCACCCGCTGGAAACTCCGCGCGTTCCGTATATCGCTTAGGtga < 1:519163/143‑1 (MQ=255) ggCGAAAGATTACGGCGTAGGTTCTGACGTTTATAGCGTGACCTCCTTCACCGAGCTGGCGCGGGATGGTCAGGATTGTGAACGCTGGAACATGCTGCACCCGCTGGAAACTCCGCGCGTTCCGTATATCGCTTAGGtgatga > 1:88286/1‑143 (MQ=255) cGAAAGGTGACGGCGTAGGTCGGGTGGTTTATAGCGTGTGCTCCCCTAGCGGGCTGGCGCGTGATGTTCAGGATTGTGAACGCTGGAACATGCTGCACCCGCTGGAAACTCCGCGCGTTCCTTATATCGCTTAGGTGATGAac < 2:1152731/143‑1 (MQ=255) ttACGGCGTAGGTTCTGACGTTTATAGCGTGACCTCCTTCACCGAGCTGGCGCGTGATGGTCAGGATTGTGAACGCTGGAACATGCTGCACCCGCTGGAAACTCCGCGCGTTCCGTATATCGCTAAGGTGATGAACGACGGTc > 1:459880/1‑143 (MQ=255) gTAGGTGGTGACGTGGATCGCGTGACCTCCTGCGCCGGGCTGGCGCGTGACGGTCAGGATTGTGAACGCTGGAACATGCTGCACCCGCTGGAGACGCCGCGCGTTCCGTATATCGCTTAGGTGATGAACGACGCTCCGGCAGt < 2:566493/143‑1 (MQ=255) tAGGTTCTGACGTTGATGGCGCGACCTCCTTCACCGAGCTGGCGCGTGATTGTCAGGATTGTGAACGCTGTAATATGCTGCCCCCGCTGGAAACGCCGCGCGTTCCGTATATCTTTTAGGTGATGAACGACGCTCCGGCAGTg < 2:1051759/143‑1 (MQ=255) gACGTTTTTAGCGTGACCTCCTTCACTGGGCTGGCCCGTGATGGTGAGGATTGTGACCGCTGGATCATGCGGCACCCGCTGGAAACTCCGCGCGTTCTGTATATCGCTTAGGTGATGAACGACGCTCCGGCAGTGGCATCTAc < 2:975845/143‑1 (MQ=255) cGTGACCTCCTTCACCGAGCTGGCGCGTGATGGTCAGGATTGTGAACGCTGGAACATGCTGCACCCGCTGGAAACTCCGCGCGTTCCGTATATCGCTTAGGTGATGAACGACGCTCCGGCAGTGGCATCTACCGACTATATGa < 1:1161878/143‑1 (MQ=255) ctttCACTGCGCTGGAGCGTGAGGGGCAGGATTGGGAACGCTGGAACATGCTGCACCCGCTGGAAACTCCGCGCGTTCCGTATATCGCTTAGGTGATGAACGACGCTCCGGCAGTGGCATCTACCGACTATATGAAACTGTTc < 1:395411/141‑1 (MQ=255) cctTCACCGAGCTGGCGCGTGAGGGTCAGGATTGTGAACGCTGGAACATGCTGCACCCGCTGGAAACTCCGCGCGTTCCGTATATCGCTTAGGTGATGAACGACGCTCCGGCAGTGGCATCTACCGACTATATGAAACTGTTc < 1:1140494/143‑1 (MQ=255) ctTCTCCGAGCTGGCGCGTGATGGTCAGGATTGTGAACGCTGGAACATGCTGCACCCGCTGGAAACTCCGCGCGTTCCGTATATCGCTTAGGTGATGAACGACGCTCCGGCAGTGGCATCTACCGACTATATGAAACTGTTCg < 2:230284/143‑1 (MQ=255) ctTCACCGGGCGGGCGCGGGATGGTCGGGATTGGGAGCGCTGGAAGATGCTGCACCCGCGGGAAGCTCCGCGCGTTCCGTATATCGCTTAGGTGATGAACGACGCTCCGGCAGTGGCATCTACCGACTATATGAAACTGTTCg < 1:435919/143‑1 (MQ=255) tCACCGAGCTGGCGCGTGATGGTCAGGATTGTGAACGCTGGAACATGCTGCACCCGCTGGAAACTCCGCGCGTTCCGTATATCGCTTAGGTGATGAACGACGCTCCGGCAGTGGCATCTACCGACTATATGAAACTGTTCGCt > 1:384322/1‑143 (MQ=255) cTGCCGCGTGATGGTCAGGATTGTGAACGCTGGAACATGCTGCACCCGCTGGAAACTCCGCGCGTTCCGTATATCGCTTAGGTGATGAACGACGCTCCGGCAGTGGCATCTACCGACTATATGAAACTGTTCGCTGAGCAGGt < 2:490657/143‑1 (MQ=255) gTCAGGATTGTGAACGCTGGAACATGCTGCACCCGCTGGAAACTCCGCGCGTTCCGTATACCGCTTAGGTGATGAACGACGCTCCGGCAGTGGCATCTACCGACTATATGACACTGTTCGCTGCGCAGGTCCGTACTTACGTa > 1:3215/1‑143 (MQ=255) tCAGGATTGTGAACGCTGGAACATGCTGCACCCGCTGGAAACTCCGCGCGTTCCGTATATCGCTTAGGTGATGAACGACGCTCCGGCAGTGGCATCTACCGACTATATGAAACTGTTCGCTGAGCAGGTCCGTACTTACGTAc < 1:593691/143‑1 (MQ=255) cAGGACTGTGAACGCTGGAACATGCTGCACCCGCTGGAAACTCCGCGCGTTCCGTATATCGCTTAGGTGATGAACGACGCTCCGGCAGTGGCATCTACCGACTATATGAAACTGTTCGCTGAGCAGGTCCGTACTTACGTAcc < 1:337807/143‑1 (MQ=255) aGGATTGTGAACGCTGGAACATGCTGCACCCGCTGGAAACTCCGCGCGTTCCGTATATCGCTTAGGTGATGAACGACGCTCCGGCAGTGGCATCTACCGACTATATGAAACTGTTCGCTGAGCAGGTCCGCACATACGTACCg > 2:519612/1‑143 (MQ=255) gcaTTTTGGACGCTCGAGCATGCGTCACCCGCTGGATACTCCTCGCGTTCCGTACAGCGCTTAGGTGATGAACGACGCTCCGGCAGTGGGCTCTACAGACTCTGTGAAACTGTTCGCTGAGCCGGTACGTAAGTACGGAcctg < 2:203955/141‑3 (MQ=255) gATTGTGAACGCTGGAACATGCTGCACCCGCTGGAAACTCCGCGCGTTCCGTATATCGCTTAGGTGATGAACGACGCTCCGGCAGTGGCATCTACCGACTATATGAAACTGTTCGCTGAGCAGGTCCGTAATGACGTAGCGGc > 1:114911/1‑143 (MQ=255) ctgtgAACGCTGGAACATGCGGCACCCGCTGGAAACTCCGCGCGTTCCGTATATCGCTTAGGTGATGAACGACGCTCCGGCAGTGGCATCTACCGACTATATGAAACTGTTCGCTGAGCAGGTCCGTACTTACGTACCGGCTg < 1:894442/142‑1 (MQ=255) aaCGCTGGCACAGGCTTCGCCCGACGTAACCTCCGCGCGTTCCGTGTATCGCTTAGGTGATGAACGACGCTCCGGCAGTGGCATCTACCGACTATAGGACACTGTTCGCTGAGCAGGTCCGTACTTACGTACCGGCTgacgac < 2:453598/143‑1 (MQ=255) aaCGCTGGAACATGCTGCACCCGCTGGAAACTCCGCGCGTTCCGTATATCGCTTAGGTGATGAACGACGCTCCGGCAGTGGCATCTACCGACTATATGAAACTGTTCGCTGAGCAGGTCCGTACTTACGTACCGGCTggcgac > 1:883705/1‑143 (MQ=255) cTGGAACATGCTGCACCCGCTGGAAACTCCGCGCGTTCCGTATATCGCTTAGGTGATGAACGACGCTCCGGCAGTGGCATCTACCGACTATATGAAACTGTTCGCTGAGCAGGTCCGTACTTACGTACCGGCTGACGACTAcc > 1:288678/1‑143 (MQ=255) ggaaacagctgcACCCGGTGGAAACCCCGGGCGTTGCGTACATCGCTTAGGTGATGAACGACGCTCCGGCAGCGGCATCTACCGACTATATGAAACTGTTCGCTGAGCAGGTCCGTACTTACGTACCGGCTGACGACTACcgc < 1:1202418/136‑1 (MQ=255) gAACATGCTGCACCCGCTGGAAACTCCGCGCGTTCCGTATATCGCTTAGGTGATGAACGACGCTCCGGCAGTGGCATCTACCGACTATATGAAACTGTTCGCTGAGCAGGTCCGTACTTACGTACCGGCTGACGACTACcgcg > 1:480438/1‑143 (MQ=255) aCATGCTGCACCCGCTGGAAACTCCGCGCGTTCCGTATATCGCTTAGGTGATGAACGACGCTCCGGCAGTGGCAGCTAACGACTATATGAAACTGTTCGCTGAGCAGGTGCGTACTTACGTATCGGCAGAAGAGTACcgagga > 2:670234/1‑139 (MQ=255) cATGCTGCACCCGCGGGAACCGCCGCGCGTTCCGTATATCGCTTAGGTGATGAACGACGCTCCGGCAGTGGCATCTACCGACTATATGAAACTGTTCGCTGAGCAGGTCCGTACTTACGTACCGGCTGACGACTACCGCGTAc < 1:1081066/143‑1 (MQ=255) tgctgtCCCCGCTGGAAACCGCGCGCGTTCCGTATATCGCGTAGGTGATGAACGACGCTCCGGCAGTGGCATCTACCGACTATATGAAACTGTTCGCTGAGCAGGTCCGTACTTACGTACCGGCTGACGACTACCGCGTACTg < 1:68375/143‑1 (MQ=255) gctgcACCCGCTGGAAACTCCGCGCGTTCCGTATATCGCTTAGGTGATGAACGACGCTCCGGCAGTGGCATCTACCGACTATATGAAACTGTTCGCTGAGCAGGTCCGTACTTACGTACCGGCTGACGACTACCGCGTACTgg < 2:384322/143‑1 (MQ=255) gcACCCGCTGGAACCTCCGCGCGTTCCGTATATCGCTTAGGTGATGAACGACGCTCCGGCAGTGGCATCTACCGACTATATGAAACTGTTCGCTGAGCAGGTCCGTACTTACGTACCGGCTGACGACTACCGCGTACTGGGTa < 1:337327/143‑1 (MQ=255) cACCCGCTGGAAACCCCGCGCGTGACGTATATCGCTTAGGTAATGAACGACGCTCCGGCAGTGGCATCTACCGACTATATGAAACTGTTCGCTGAGCAGGTCCGTACTTACGTACCGGCTGACGACTACCGCGTACTGGGTAc < 2:329807/143‑1 (MQ=255) tggCTGGAAACGCCGCTCGTTCCGTATATCGCTTAGGTGATGAACGACGCTCCGGCAGTGGCATCTACCGACTATATGAAACTGTTCGCTGAGCAGGTCCGTACTTACGTACCGGCTGACGACTACCGCGTACTGGGTACTGa < 2:879175/141‑1 (MQ=255) gCTGGAAACTCCGCGCGTTCCGTATATCGCTTAGGTGATGAACGACGCTCCGGCAGTGGCATCTACCGACTATATGAAACTGTTCGCTGAGCAGGTCCGTACTTACGTACCGGCTGACGACTACCGCGTACTGGGTACTGATg > 1:24475/1‑143 (MQ=255) gCTGGAAACTCCGCGCGTTCCGTATATCGCTTAGGTGATGAACGACGCTCCGGCAGTGGCATCTACCGACTATATGAAACTGTTCGCTGAGCAGGTCCGTACTTACGTACCGGCTGACGACTACCGCGTACTGGGTACTGATg > 1:506316/1‑143 (MQ=255) gAAACTCCGCGCGTTCCGTATATCGCTTAGGTGATGAACGACGCTCCGGCAGTGGCATCTACCGACTATATGAAACTGTTCGCTGAGCAGGTCCGTACTTACGTACCGGCTGACGACTACCGCGTACTGGGTACTGATGGCtt < 1:905407/143‑1 (MQ=255) gcTCCGCGCGTTCCGTATCTCGCTCTGGTGATGAACGACGCTCCGGCAGTGGCGTCTACCGACTATATGAGACTGTTCGCTGAGCAGGTCCGTACTTACGTACCGGCTGACGACTACCGCGTACTGGGTACTGATGGCTTCgg < 2:683561/142‑1 (MQ=255) gcgcgTTCCGTATATCGCTTAGGTGATGAACGACGCTCCGGCAGTGGCATCTACCGACTATATGAAACTCTTCGCTGAGCAGGTCCGTACTTACGTACCGGCTGACGACTACCCCGTACTGGGTACTGATGGCTTCGGGCGtt > 1:933471/1‑143 (MQ=255) cgcgTTCCGTATATCGCTTAGGTGATGAACGACGCTCCGGCAGTGGCATCTACCGACTATATGAAACTGTTCGCTGAGCAGGTCCGTACTTACGTACCGGCTGACGACTACCGCGTACTGGGTACTGATGCCTTCGGTCGTTc > 2:869640/1‑143 (MQ=255) atatCGCTTAGGTGATGAACGACGCTCCGGCAGTGGCATCTACCGACTATATGAAACTGTTCGCTGAGCAGGTCCGTACTTACGTACCGGCTGACGACTACCGCGTACTGGGGACTGATGGCTTCGGTCGTTCCGACAGCCGt > 1:1032331/1‑143 (MQ=255) atatCGCTTAGGTGAGGAACGACGCTCCGGCAGTGGCATCTACCGACTATATGAAACTGTTCGCTGAGCAGGTCCGTACTTACGTACCGGCTGACGACTACCGCGTACTGGGTACTGATGGCTTCGGTCGTTCCGACAGCCGt < 1:527950/143‑1 (MQ=255) atCGCTTAGGTGATGAACGACGCTCCGGCAGTGGCATCTACCGACTATATGAAACTGTTCGCTGAGCAGGTCCGTACTTACGTACCGGCTGACGACTACCGCGTACTGGGTACTGATGGCTTCGGTCGTTCCGACAGCCGTga < 1:857036/143‑1 (MQ=255) cGCTTAGGTGATGAACGACGCTCCGGCAGTGGCATCTACCGACTATATGAAACTGTTCGCTGAGCAGGTCCGTACTTACGTACCGGCCGACGACTACCGCGTACTGGGGACTGATGGCGTCGGCCGTCCCGACAGCCGTgagc > 2:948917/1‑142 (MQ=255) | AGATCCTGGCGAAAGATTACGGCGTAGGTTCTGACGTTTATAGCGTGACCTCCTTCACCGAGCTGGCGCGTGATGGTCAGGATTGTGAACGCTGGAACATGCTGCACCCGCTGGAAACTCCGCGCGTTCCGTATATCGCTCAGGTGATGAACGACGCTCCGGCAGTGGCATCTACCGACTATATGAAACTGTTCGCTGAGCAGGTCCGTACTTACGTACCGGCTGACGACTACCGCGTACTGGGTACTGATGGCTTCGGTCGTTCCGACAGCCGTGAGA > NC_000913/125247‑125525 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |