| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 2,132,787 | A→C | I204S (ATC→AGC) | wcaA ← | putative colanic acid biosynthesis glycosyl transferase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 2,132,787 | 0 | A | C | 98.2% | 159.4 / NA | 57 | I204S (ATC→AGC) | wcaA | putative colanic acid biosynthesis glycosyl transferase |
| Reads supporting (aligned to +/- strand): ref base A (0/0); major base C (26/30); minor base T (0/1); total (26/31) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.90e-01 | |||||||||||
GGATCTGGTAGAGGGTAAACAGCTGATATTTTTTGCTGGCGCGGTCGAATTTGTCTTTGTGCTTGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGATCTGCGTCGCCTCTTCTACTTTCCACGGT‑TCGCCGTACTCCACCACCATCCGCAGGAAGATGTCGTAATCCTGTGCCGCTTTCAGTTCGGTATCGAACAAACACTCTTTGAAACGCCATGCCCAGGTAAAGACCTGGTT > NC_000913/2132647‑2132926 | ggATCTGGTAGAGGGTAAACAGCTGATATTTTTTGCTGGCGCGGTCGAATTTGGCTTTGTGCTTGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATCTGCAGGCTc > 2:255127/1‑143 (MQ=255) gTAGAGGGTAAACAGCTGATATTTTTTGCTGGCGCGGTCGAATTTGTCTTTGTGCTTGCGGTAAAAGTGGAAGTACCCGGAGAGTACGGCACTTGAGAGGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCg > 1:874317/1‑143 (MQ=255) tAGAGGGTAAACAGCTGATATTTTTTGCTGGCGCGGTCGAATTTGTCTTTGTGCTTGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGc > 1:916745/1‑143 (MQ=255) ggTACACAGCTGATATTTTTTGCTGGCGCGGTCGAATTTGTATTTGTGCTTGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCtctt > 2:488692/1‑143 (MQ=255) ggTAAACAGCTGATATTTTTTGCTGGCGCGGTCGCATTTGTCTTTGTGCTTGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATGTCACCGTGATTGATATGCAGGCTCTGCGTCGCctcct > 1:529572/1‑141 (MQ=255) aCAGCTGATATTTTTTGCTGGCGCGGTCGAATTTGTATTTGTGATTGCGGTAAAAGTGGAAGTACACGGAGAATTTTTTCGGTGACGAGGTGATATGCATTTCAACGTGATTGATATGCAGGCTCTGCGTAGCCTGTTCTaat > 2:439031/1‑141 (MQ=255) ggATATTTTTTGCTGGCGCGGTCGCATTTGTGTGTGTGCTTGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTGCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCa < 2:412778/142‑1 (MQ=255) atatTTTTTGCTGGCGCGGTCGAATTTGTCTTTGTGCTTGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGGTTGATAGGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACg > 1:829867/1‑143 (MQ=255) atatTTTTTGCTGGCGCGGTCGAATTTGTCTTTGTGCTCGCGGTAAAAGTGGAAGTACCCGGAGCATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATGGATATGCAGGCTCTGCGTCGCCGCTTCTACTCTCCACg > 1:473610/1‑143 (MQ=255) atatCTTTTGCTGGCGCGGTCGAATTTGTCTTTGTGCTTGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACtttgcccg > 2:327014/1‑138 (MQ=255) tttgttGCTGGCGCGGTCGAATTTGTCTTTGTGCTTGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGt‑t < 1:733861/143‑1 (MQ=255) ttttGCTGGCGCGGTCGAATTTGTCTTTGTGCTTGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGT‑TCg < 1:852193/143‑1 (MQ=255) ttGCTGGCGCGGTCGAATTTGTCTTTGTGCTTGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGT‑TCGcc < 2:179005/143‑1 (MQ=255) ggCTGGGGCGGAGGGAGTTGGCTATGGGCGTGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGT‑TCGCCg < 1:13253/142‑1 (MQ=255) gCTGGCGCGGTCGAATTTGTCTTTGTGCTTGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGT‑TCGCCGt < 1:790906/143‑1 (MQ=255) tGGCGCGGTCGAATGTGTCTTTGTGCTTGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGT‑TCGCCGTAc < 2:803385/143‑1 (MQ=255) agctgTCGTATTTGTCTGTGTCGTTGCGGTAAAGGGGGCAGTACCTGGAGAATTTTTTCGTTGGCGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCTACGGT‑TCGCCGTACTcc < 2:30798/139‑1 (MQ=255) cgGTCGAATTTGTCTTTGTGCTTGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGT‑TCGCCGtcctcccc > 1:963571/1‑141 (MQ=255) gTCGAATTTGTCTTTGTGCTTGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGCTCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTACACGGT‑TCGCCGTACTccacca > 2:602701/1‑143 (MQ=255) gAATTTGTCTTTGTGCTTGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATTCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGT‑TCGCCGTACTccacccccc > 1:554298/1‑142 (MQ=255) aTTTGTCTTTGTGCTTGCGGTAAAAGTGGAAGTACCCGGAGAAGTTTTTCGGGGACGAGGTGATCTGCATTGCACCGTGATTGATATGCAGGCTCTGGGTGGCCTCTTCTACTTTCCACGGT‑TCGGCGCACTCCGCCCCCATc > 2:620790/1‑143 (MQ=255) tCTTTGTGATTCCTGTTAAAGTGGAAGTACCCGGAGAATTTTTTCGTTGACGATGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGTCTCTTCTACTTTCCACGGT‑TCGCCGTACTCCACCACCATCCGCAg < 2:107336/143‑1 (MQ=255) tttGTGCTTGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGC‑TCGCCGTACTCCACCACCATCCGCAGGa > 1:118145/1‑143 (MQ=255) tttGTGCTTGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTGCTACTTTCCACGGT‑TCGCCGTACTCCACCACCGTCCGCAGGa > 1:60618/1‑143 (MQ=255) tttGTGCTTGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGAATGTTCTACTTGCCACGGT‑TCGCCTCACTCCACCACCATCCGCAGGa > 1:1188795/1‑143 (MQ=255) gcatGCGTTTAAAGTTGAATCACCCCGAAAATGTTTTCGCTTACGATGTGATCGTCATCTCACCGTGATTGATATGCAGGCTCTTCGTCGCCTCTGCCACTTTCCACACT‑TCCCCTTACTCCACCACCATCCTCAGTAAGATg < 1:829329/140‑1 (MQ=255) ttGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGT‑TCGCCGTACTCCACCACCATCCGCAGGAAGATGTc > 1:985495/1‑143 (MQ=255) ttGCGGGAAGAGTGGAATTACCCGGAGAGTTTTCTCGATTACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCGACTTGCCACGGT‑TCGCCGTACTCCACCACCATCCGCAGGAAGATGTc < 2:526022/143‑1 (MQ=255) cGGTAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGT‑TCGCCGTACTCCACCACCATCCGCAGGAAGATGTCGTa < 1:240034/143‑1 (MQ=255) ggtacgaGTGGATCTGCCCGCAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGAGATGCAGGCTCTGCGTCGCCTCTTCTACTTGCCACGGT‑TCGCCGTACTCCACCACCATCCGCAGGAAGATGTCGTaa < 2:1108299/137‑1 (MQ=255) ggTAAAAGGGGAAGTACCCGGAGAATGGTTTCGGTGACGAGGTGATCTGCAGTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGT‑TCGCCGTACTCCACCACCATCCGCAGGAAGATGTCGTaa < 1:655313/143‑1 (MQ=255) gTAGAAGGGGAAGTACCCGGAGAAGTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGT‑TCGCCGTACTCCACCACCATCCGCAGGAAGATGTCGTAAt < 1:494456/143‑1 (MQ=255) aaaGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCAGCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTACACGGT‑TCGCCGTACTCCACCACCATCCGCCGGCAGATGTCGTAATCCt > 1:149008/1‑143 (MQ=255) tGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCAACGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGT‑TCGCCGTACTCCACCACCATCCGCAGGAAGATGTCGTAATCCTGTGc > 1:1107268/1‑143 (MQ=255) tgcccGGAGAACTTTCTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTACCCCTTTCCGTGGT‑TCGCCGTACTCCACCACCATCCGCAGGAAGATGTCGTAATCCTGTGCCGCttt < 1:327014/141‑1 (MQ=255) tACCCGGCGTCTTTTTTCGGTGACGCGGTTATCTGGATTTTACCGTGATTGATATGTAGGTTCTGCGTTTCCTGTTCTACTTTCCACGGT‑TCGCCGTACTCCACCACCTTCCGTAGGAAGATGTCGTAATCCTGTGCCGCttt < 2:79173/143‑1 (MQ=255) tACCCGGAGACTTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGT‑TCGCCGTACTCCACCACCATCCGCAGGAAGATGTCGTAATCCTGTGCCGCttt < 1:592409/143‑1 (MQ=255) gcccGGAGAATGTTCTCGGTGACGGGCGGATCTGCATTTCGCCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGT‑TCGCCGTACTCCACCACCATCCGCAGGAAGATGTCGTAATCCTGTGCCGCTTTc < 1:1128532/142‑1 (MQ=255) ccccGAGAATTGTTTCGGTGGCGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGT‑TCGCCGTACTCCACCACCATCCGCAGGAAGATGTCGTAATCCTGCGCCGCTTTCa < 2:918296/143‑1 (MQ=255) cccGTAGAATTTTGTCGGTGACGAGGTGATCTGCATGTCACCGTGAGTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGT‑TCGCCGTACTCCACCACCATCCGCAGGAAGATGTCGTAATCCTGTGCCGCTTTCa < 2:1135059/143‑1 (MQ=255) gcagaATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTTCGTCGCCTCTTCTACTTTCCACGGT‑TCGCCGTACTCCACCACCATCCGCAGGAAGATGTCGTAATCCTGTGCCGCTTTCAGtt < 1:248334/141‑1 (MQ=255) agagTTTTTTCGGTGACGAGGTGATCTGCCTTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGT‑TCGCCGTACTCCACCACCATCCGAAGTAAGATATCGTAATCCTGTGCCGCTTTCAGTTCg < 2:662652/143‑1 (MQ=255) ttttttCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGT‑TCGCCGTACTCCACCACCATCCGCAGGAAGATGTCGTAATCCTGTGCCGCTTTCAGTTCGGTAt > 2:40894/1‑143 (MQ=255) ttCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGT‑TCGCCGTACTCCACCACCATCCGCAGGAAGATGTCGTAATCCTGTGCCGCTTTCAGTTCGGTATCGaa < 1:426460/143‑1 (MQ=255) ttCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCGGCGTCGCCTCTTCTACTTTCCACGGT‑TCGCCGTACTCCACCACCATCCGCAGGAAGATGTCGTAATCCTGTGCCGCTTTCAGTTCGGTATCGaa < 1:810881/143‑1 (MQ=255) tgTGATCTGCATTTCACCGTGTTTGACATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGT‑TCGCCGTACTCCACCACCATCCGCAGGAAGATGTCGTAATCCTGTGCCGCTTTCAGTTCGGTATCGAACAAACACTCtt < 2:165000/142‑1 (MQ=255) ggtggtCTGCATTCCACCGTGTATGACACGCAGGCTCTCCGTCGCCTCTTCTCCTTTCCACGGT‑TCGCCGTCCTCCACCACCATCCGCAGGAAGATGCCGTAATCCTGTGCCGCTTTCAGTTCGGTAGCGAACAAACACTCtt < 2:1013325/143‑1 (MQ=255) ggTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGT‑TCGCCGTACTCCACCACCATCCGCAGGAAGATGTCGTAATCCTGTGCCGCTTTCAGTTCGGTATCGAACAAACACTCtt > 1:947067/1‑143 (MQ=255) ggTGATCTGCATTTCACCGTGAATGAAAAGCAGGCTCTGAGTCCACTC‑ACGAATGTCTGTATTGTTGCCATACACTATCACCAACCGCAGGAAGAAGTCGTAATCCGGTGCCGCTTTCAGTTCGGTATCGaataaaagcgctt > 2:252945/1‑136 (MQ=255) gTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGT‑TCGCCGTACTCCACCACCATCCGCAGGAAGATGTCGTAATCCTGTGCCGCTTTCAGTTCGGTATCGCACAAACACTCttt > 1:280391/1‑143 (MQ=255) cTGCATTCCACCCTGGTTGTTTTGCAGGCTCTTCCTCGCCTCTTCTACTTTCTACGGT‑TCGCCGTACTCCACCACCATCCGCAGGAAGATGTCGTAATCCTTTGCCGCTTTCAGTTCGGTATCGAACAAACACTCTTTGAAAc < 2:329146/143‑1 (MQ=255) cTGCAGTCCCCCGTGATTGAGATGCAGGCTCTGCGTCGCCTCGTCTACTTTCCACGGG‑TCGCCGTACTCCACCACCATCCGCATGAAGATGTCGTAATCCTGTGCCGCTTTCAGTTCGGTATCGAACAAACACTCTTTGAAAc < 2:1067829/143‑1 (MQ=255) aCCGTGATTGATATGCAGGCTCTGCGTCGCCCCTTCTACTTTCCACGGT‑TCGCCGTACTCCACCACCATCCGCAGGAAGATGTCGTAATCCTGTGCCGCTTTCAGCTCGGCACCGAACCCACCCTCTTTGAACCGCCCTGccc > 2:168225/1‑143 (MQ=255) ccGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGT‑TCGCCGTACTCCACCACCATCCGCAGGAAGATGTCGTAATCCTGTGCCGCTTTCAGTTCGGTATCGAACGAACACTCTTTGAAACGCCGTGcaca > 2:633020/1‑143 (MQ=255) gatATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGT‑TCGCCGTACTCCACCACCATCCGCAGGAAGATGTCGTAATCCTGTGCCGCTTTCAGTTCGGTATCGAACAAACACTCTTTGAAACGCCATGCCCAGGTAAAGa < 1:1045966/143‑1 (MQ=255) tatGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGT‑TCGCCGTACTTCACCACCATCCGCAGGAAGATGTCGTAATCCTGTGCCGCGTTCAGTTCGGTATCGAACAAACACTCTTTGAAACGCCATGCCCAGGTAAAGAcc < 1:389855/143‑1 (MQ=255) aGGCTCTGCGTCGCCTCCTCTACTTTCCACGGT‑TCGCCGTACTCCACCACCATCCGCAGGAAGATGTCGTAATCCTGTGCCGCTTTCAGTTCGGTATCGAACAAACACTCTTTGAAACGCCATGCCCAGGGAAAGACCAGGtc > 1:690354/1‑142 (MQ=255) | GGATCTGGTAGAGGGTAAACAGCTGATATTTTTTGCTGGCGCGGTCGAATTTGTCTTTGTGCTTGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGATCTGCGTCGCCTCTTCTACTTTCCACGGT‑TCGCCGTACTCCACCACCATCCGCAGGAAGATGTCGTAATCCTGTGCCGCTTTCAGTTCGGTATCGAACAAACACTCTTTGAAACGCCATGCCCAGGTAAAGACCTGGTT > NC_000913/2132647‑2132926 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |