Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	800,807	G→T	A17S (GCG→TCG)	ybhH →	putative isomerase YbhH

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	800,807	0	G	T	98.6%	206.1 / ‑5.7	71	A17S (GCG→TCG)	ybhH	putative isomerase YbhH
Reads supporting (aligned to +/- strand):  ref base G (0/0);  major base T (31/39);  minor base A (0/1);  total (31/40)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.44e-01

TATTAATCGTTGAGCTAAAGTCATTAGAGATGCTTTGCCCTTAATGTAACCATATCGCAATAAGTTATGTTTTTAAATTGAGGGCATTATTATGAAAAAAATACCCTGCGTGATGATGCGAGGTGGAACCTCGAGGGGCGCGTTCCTGTTAGCGGAACATTTACCCGAAGATCAAACGCAGCGCGATAAAATATTGATGGCAATTATGGGTTCCGGTAACGATCTGGAAATTGACGGTATTGGCGGCGGTAATCCACTGACCAGTAAAGTCGCCATTATT  >  NC_000913/800668‑800947                                                                                                                                            |                                                                                                                                             tattaATCGTTGAGCTAAAGTCATTAGAGATGCTTTGCCCTTAATGTAACCATATCGCAATAAGTTATGTTTTTAAATTGAGGGCATTATTATGCAAAAAATACCCTGCGTGATGATGCGAGGTGGAACCTCGAGGGGCTCGt                                                                                                                                           >  1:818070/1‑143 (MQ=255)  attaATCGTTGAGCTAAAGTCATTAGAGATGCTTTGCCCTTAATGTAACCATATCGCAATAAGTTATGTTTTTAAATTGAGGGCATTATTATGAACAAAGGTTTCTGCGTGATGATGCGAGGTGGAACCTCGAGGGGCTCGtt                                                                                                                                          >  2:847098/1‑143 (MQ=255)   taagacGTGGAGGTAAAGTCAGTAGAGATGCTCTTGCTTTAATGTAGTGTAATTTCAAGTGGTGATGTGCAAAAAGTGCGGGCATTAGTATGAAAAAAACACCCTGCGTGATGATGCGAGGTGGAACCTCGAGGGGCTCGTTc                                                                                                                                         <  2:850820/138‑1 (MQ=255)     aagcGTTGAGCGAAAGTCATTAGAGATGCTTTGCCCTTAATGTAACCATATCGCAATAAGTTATGTTTTTAAATTGAGGGCATTATTATGAAAAAAATACCCTGCGTGATGATGCGAGGTGGAACCTCGAGGGGCTCGTTCCt                                                                                                                                       <  1:884493/140‑1 (MQ=255)     aaTCTTTGATCTAAAGTCATTAGAGATGCATTTCCCTTAATTTAACCATATCTCAATAATTTATTTTTCTAAAACAAAAACAATAAAAAAAAAAAAATACCCTGCGTGATGATGCGAGGTGGAACCTCGAGGGGCTCGTTCCt                                                                                                                                       <  1:588487/143‑1 (MQ=255)     aaTCGTTGAGCTAAAGTCATTAGAGATGCTTTGCCCTTAATGTAACCATATCGCAATAAGTTATGTTTTTAAATTGAGGGCATTATTATGAAAAAAATACCCTGCGTGATGATGCGAGGTGGAACCTCGAGGGGCTCGTTCCt                                                                                                                                       >  1:74312/1‑143 (MQ=255)         gTTGAGCTAAAGTCATTAGAGATGCTTTGCCCTTAATGTAACCATATCGCAATAAGTTATGTTTTTAAATTGAGGGCATTATTATGAAAAAAATCCCCTGCGTGATGCTGCGAGGTGGAACCTCGAGGGGCTCGTTCCTGTTa                                                                                                                                   >  1:3241/1‑143 (MQ=255)         gTTGAGCTAAAGTCATTAGAGATGCTTTGCCCTTAATGTAACCATATCGCAATAAGTTATGTTTTTAAATTGAGGGCATTATTATGAAAAAAATACCCTGCGTGATGATGCGAGGTGGAACCTCGAGGGGCTCGTTCCTGTTa                                                                                                                                   <  1:825844/143‑1 (MQ=255)         gTTGAGATAAAGTTATTAGAGATGCTTTGTATTTAATGTAACCATATCGCAATAAGTTATGTTTTTAAATTGAGGGCATTATTATGAAAAAAATACCCTGCGTGATGATGCGAGGTGGAACCTCGAGGGGCTCGTTCCTGTTa                                                                                                                                   <  2:465920/143‑1 (MQ=255)          ttaggCTAAAGTGAGTAGAGATGCTTTGCCCTTAATGTAACCATATCGAAAGAAGTTATGTTTTGAAATTGAGGGCATTATTATGAAAAAAATACCCTGCGTGATGATGCGAGGTGGAACCTCGAGGGGCTCGTTCCTGTTAg                                                                                                                                  <  2:767752/139‑1 (MQ=255)            gAGCTAAAGTCATTAGAGATGCTTTGCCCTTAATGTAACCATATCGCAATAAGTTATGTTTTTAAATTGAGGGCATTATTATGAAAAAAATACCCTGCGTGATGATGCGAGGTGGAACCTCGAGGGGCTCGTTCCTGTTAGCg                                                                                                                                <  1:76456/143‑1 (MQ=255)                  aaGTCATTAGAGATGCTTTGCCCTTTATGTAACCATATCGCAATAAGTTATGTTTTTAAATTGAGGGCATTATTATGAAAAAAATACCCTGCGTGATGATGCGAGGTGGAACCTCGAGGGGCTCGTTCCTGTTAGCGGAACAt                                                                                                                          >  2:371936/1‑143 (MQ=255)                       aTTAGCGATGCTTTGCCCTTAATGTAACCATATCGCAATAAGTTATGTTTTTAAATTGAGGGCATTATTATGAAAAAAATACCCTGCGTGATGATGCGAGGTGGAACCTCGAGGGGCTCGTTCCTGTTAGCGGAACATTTAcc                                                                                                                     <  1:1024034/143‑1 (MQ=255)                         tAGAGATGCTTTGCCCTTAATGTAACCATATCGCACTAAGTTATGTTTTTAAATTGAGGGCATTATTATGAAAAAAATACCCTGCGTGATGATGCGAGGTGGAACCTCGAGGGGCTCGTTCCTGTTAGCGGAACATTTACCCg                                                                                                                   >  1:631867/1‑143 (MQ=255)                           gagaTGCTTTGCCCTTAATGTAACCATATCGCAATAAGTTATGTTTTTAAATTGAGGGCATTATTATGAAAAAAATACCCTGCGTGATGATGCGAGGTGGAACCTCGAGGGGCTCGTTCCTGTTAGCGGAACGTTTACCCGaa                                                                                                                 >  1:470868/1‑143 (MQ=255)                            agaTGCTTTGCCCTTAATGTAACCATATCGCAATAAGTTATGTTTTTAAATTGAGGGCATTATTATGAAAAAAATACCCTGCGTGATGATGCGAGGTGGAACCTCGAGGGGCTCGTTCCTGTTAGCGGAACATTTACCCGAAg                                                                                                                >  1:115105/1‑143 (MQ=255)                            agaTGCGTGGCGCGTAATGGAAGCAGAGGGCAATAAGTTATGGGTGTAAATTGAGGGCATGATTATGAAAAGAAGACCCTGCGTGATGATGCGAGGTGGAACCTCGAGGGGCTCGTTCCTGTTAGCGGAACATTTACCCGAAg                                                                                                                <  2:166614/143‑1 (MQ=255)                                  tttGCCCTTAATGTAACCATATCGCAATAAGTTATGTTTTTAAATTGAGGGCATTATTATGAAAAAAATACCCTGCGTGATGATGCGAGGTGGAACCTCGAGGGGCTCGTTCCTGTTAGCGGAACATTTACCCGAAGATCaaa                                                                                                          <  2:537468/143‑1 (MQ=255)                                      cccTGAATGGAACCAGAGCCGAATGAGTTATGGTGTTAGATTCAGTGCATTATTATGAAAAGAATACCCTGCGTGATGATGCGAGGTTGAACCTCGAGGGGCTCGTTCCTGTTAGCGGAACATTTACCCGAAGATCAAACGCa                                                                                                      <  2:1138951/143‑1 (MQ=255)                                       ccTTAATGTAACCATATCGCAATAAGTTATGTTTTTAAATTGAGGGCATTATTATGAAAAAAATACCCTGCGTGATGATGCGAGGTGGAACCTCGAGGGGCTCGTTCCTGTTAGCGGAACATTTACCAGAAAAACAAACCCAg                                                                                                     >  1:740968/1‑143 (MQ=255)                                        cTTAATGTAACCATATCGCAATAAGTTATGTTTTTAAATTGAGGGCATTATTATGAAAAAAATACCCTGCGTGATGATGCGAGGTGGAACCTCGAGGGGCTCGTTCCTGTTAGCGGAACATTTACCCGAAGATCAAACGCAgc                                                                                                    >  1:231574/1‑143 (MQ=255)                                              gTAACCATATCGCAATAAGTTATGTTTTTAAATTGAGGGCATTATTATGAAAAAAATACCCTGCGTGATGATGCGAGGTGGAACCTCGAGGGGCTCGTTCCTGTTAGCGGAACATTTACCCGAAGATCAAACGCAGCGCGATa                                                                                              <  1:118316/143‑1 (MQ=255)                                                   cATATCGCAATAAGTTATGTTTTTAAATTGAGGGCATTATTATGAAAAAAATACCCTGCGTGATGATGCGAGGTGGAACCTCGAGGGGCTCGTTCCTGTTAGCGGAACATTTACCCGAAGATCAAACGCAGCGCGATAAActa                                                                                         >  1:371501/1‑143 (MQ=255)                                                   cATATCGAAATAAGTTATGTTTTTAAATAGAGGGCATTATTATGAAAAAAATACCCTGCGTGATGATGCGAGGTGGAACCTCGAGGGGCTCGTTCCTGTTAGCGGAACATTTACCCGAAGATCAAACGCAGCGCGATAAAata                                                                                         <  1:731527/143‑1 (MQ=255)                                                    atatCGCAATAAGTTATGTTTTTAAATTGAGGGCATTATTATGAAAAAAATACCCTGCGTGATGATGCGAGGTGGAACCTCGAGGGGCTCGTTCCTGTTAGCGGAACATTTACCCGAAGATCAAACGCCGCGCGATCAAatcc                                                                                        >  1:433435/1‑141 (MQ=255)                                                    atatCGCAATAAGTTATGTTTTTAAATTGAGGGCATTATTATGAAAAAAATACCCTGCGTGATGATGCGAGGTGGAACCTCGAGGGGCTCGTTCCTGTTAGCGGAACATTTACCCGAAGATCAAACGCAGCGCGATAAAatat                                                                                        >  2:740401/1‑143 (MQ=255)                                                     tatCGCAATAAGTTATGTTTTTAAATTGAGGGCATTATTATGAAAAAAATACCCTGCGTGATGATGCGAGGTGGAACCTCGAGGGGCTCGTTCCTGTTAGCGGAACATTTACCCGAAGATCAAACGCAGCGCGATAAAATAtt                                                                                       <  1:1056834/143‑1 (MQ=255)                                                        cGCAATAAGTTATGTTTTTAAATTGAGGGCATTATTATTAAAAAAATACCCTGCGTGATGATGCGAGGTGGAACCTCGAGGGGCTCGTTCCTGTTAGCGGAACATTTACCCGAAGATCAAACGCAGCGCGATAAAATATTGAt                                                                                    <  1:694633/143‑1 (MQ=255)                                                         gCAATAAGTTATGTTTTTAAATTGAGGGCATTATTATGAAAAAAATACCCTGCGTGATGATGCGAGGTGGAACCTCGAGGGGCTCGTTCCTGTTAGCGGCACATTTACCCGAAGATCAAACGCAGCGCGATTACATCttgttg                                                                                   >  1:68364/1‑143 (MQ=255)                                                             tAAGTTATGTTTTTAAATTGAGGGCATTATTATGAAAAAAATACCCTGCGTGATGATGCGAGGTGGAACCTCGAGGGGCTCGTTCCTGTTAGCGGAACATTTACCCGAAGGTCAAACGCAGCGCGATAAAATATTGATGGCac                                                                               >  2:752102/1‑142 (MQ=255)                                                                  tggtatattAAATTGAGGGGATTATTATGAAGAAAGGACCCTGGTTGGTGATGCGAGGTGAAACCTCGAGGGGCTCGTTCCTGTTAGCGGAACATTTACGCGAAGAGCAAACGCAGCGCGATAAAATATTGATGGCAATTATg                                                                          <  2:967090/136‑1 (MQ=255)                                                                   aggTTTTTAAATTGAGGGCATTATTATGAAAAAAATAACCTGAGTGATGATGCGAGGTGGAACCTCGAGAGGATCCTTCCTGTTAGCAGAACAATTACCCGAAGATCCAACGCAGCACGATCAACGACTGATGGCCACTAtca                                                                         >  2:496628/3‑141 (MQ=255)                                                                   aTGTTTTTAAATTGAGGGCATTATTATGAAAAAAATACCCTGCGTGATGATGCGAGGTGGAACAAAGAGGGGCTCGTTCCTGTTAGCGGAACATTTACCCGAAGATCAAACGCAGAGCGATAAAAAATTGATGGCAATAATgg                                                                         >  2:1172840/1‑143 (MQ=255)                                                                        tttAAATTGAGGGCATTATTATGAAAAAAATACCCTGCGTGATGATGCGAGGTGGAACCTCGAGGGGCTCGTTCCTGTTAGCGGAACATTTACCCGAAGATCAAACGCAGCGCGAGAGAATATTGATGGCAATTATGGGTTca                                                                    >  1:1013993/1‑142 (MQ=255)                                                                         ttAAATTGAGGGCATTATTATGAAACCAACAAAAGCCGTTATTATGCGAGGTGGAACCTCGAGGGGCTCGTTCCTGTTAGCGGAACATTTACCCGAAGATCAAACGCAGCGCGATAAAATATTGATGGCAATTATGGGTTCCg                                                                   <  1:813180/143‑1 (MQ=255)                                                                           aaaTTGAGGGCATTATTATGAAAAAAATACCCTGCGTGATTATGCGAGGTGGAACCTCGAGGGGCTCGTTCCTGTTAGCGGAACATTTACCCGAAGAGCAAACGCAGCGCGATAAAATATTGGTGGCAACGATGGGTTCCGGt                                                                 >  1:149287/1‑143 (MQ=255)                                                                            aaTTGAGGGCATTATTATGAAAAAAATACCCTGCGTGATGATGCGAGGTGGAACCTCGAGGGGCTCGTTCCTGTTAGCGGAACATTTACCCGAAGATCAAACGCAGCGCGATAAAATATTGATGGCAATAATGGGTTGCGGtc                                                                >  2:332635/1‑142 (MQ=255)                                                                             attagtggCAGTGTTATGCAAAAAAGACCCTGGGTGATGATGCGAGGTGGAACCTCGAGGGGCTCGTTCCTGTTAGCGGAACATTTACCCGAAGATCAAACGCAGCGCGATAAAATATTGATGGCAATTATGGGTTCCGGTaa                                                               <  2:510881/137‑1 (MQ=255)                                                                             aTTGGGCGCATTATTATGAAAAAAATACCCTGCGTGATGATGCGAGGTGGAACCTCGAGAAACATCTCCAAACGAATTAAGTGTTTTCCCCAAGACCAAACCCAGCGCGATAAAATATTGATGGCAATTATGGGTTCCGGTaa                                                               <  1:881085/143‑1 (MQ=255)                                                                             aTTGAGGGCATTATTATGAAAAAAATACCCTGCGTGATGATGCGAGGTGGAACCTCGAGGGGCTCGTTCGTGTTAGCGGAACATTTACCCGAAGATCAAACGCGGCGCGATAAAATATAGATGGCAATTATAGGTTCCGGCaa                                                               >  2:374868/1‑143 (MQ=255)                                                                                   ggCATTATGATGAAAAAAATACCCTGCGTGATGATGCGAGGTGGAACCTCGAGGGGCTCGTTCCTGTTAGCGGAACATTTACCCGAAGATCAAACGCAGCGCGATAAAATATTGATGGCAATTATGGGTTCCGGTAACGATCt                                                         <  1:559900/143‑1 (MQ=255)                                                                                        tattatGAAAAAAATACCCTGCGTGATTATGCGAGGTGGAACCTCGAGGGGCTCGTTCCTGTTAGCGGAACATTTACCCGAAGATCAAACGCAGCGCGATAAAATATTGATGGCAATTATGGGTTCCGGTAACGATCTGGaaa                                                    <  1:713673/143‑1 (MQ=255)                                                                                        gattatGAAAAACATCCCCTGCGTGATGATGCGAGGTGGAACCTCGAGGGGCTCGTTCCTGTTAGCGGAACATTTACCCGAAGATCAAACGCAGCGCGATAAAATATTGATGGCAATTCTGGGTTCCGGTAACGATCTGGaaa                                                    <  1:208506/142‑1 (MQ=255)                                                                                          ttatGAAAAAAATACCCTGCGTGATGATGCGAGGTGGAACCTCGAGGGGCTCGTTCCTGTTAGCGGAACATTTACCCGAAGATCAAACGCAGCGCGATAAAATATTGATGGCAATTATGGGTTCCGGTAACGATCTGGAAAtt                                                  <  1:371936/143‑1 (MQ=255)                                                                                           tatGAAAAAAATACCCTGCGTGATGATGCGAGGTGGAACCTCGAGGGGCTCGTTCCTGTTAGCGGAACATTTACCCGAAGATCAAACGCAGCGCGATAAAATATTGATGGCAATTATGGGTGCCGGTAGCGATCTGGAACttc                                                 >  2:281354/1‑142 (MQ=255)                                                                                            agcaaaaaaaTACCCTGCGTGATGATGCGAGGTGGAACCTCGAGGGGCTCGTGCCTGTTAGCGGAACATTGACCCGAAGATCAAACGCAGCGCGATAAAATATTGATGGCAATTATGGGTTCCGGTAACGATCTGGAAATTGa                                                <  2:1134029/140‑1 (MQ=255)                                                                                             tGAAAAAACTGCCCTGCGTGATGATGCGAGGTGGAACCTCGAGGGGCTCGTGCCTGTTAGCGGAACATTTACCCGAAGATCAAACGCAGCGCGATAAAATATTGATGGCAATTATGGGTTCCGGTAACGATCTGGAAATTGAc                                               <  2:1183401/143‑1 (MQ=255)                                                                                              gAAAAAAATACCCTGCGTGATGATGCGAGGTGGAACCTCGAGGGGCTCGTTCCTGTTAGCGGAACATTTACCCGAAGATCAAACGCAGCGCGATAAAATATTGATGGCAATTATGGGTTCCGGTAACGATCTGGAAATTGACg                                              <  2:322721/143‑1 (MQ=255)                                                                                                      tACCCTGCGTGATGATGCGAGGTGGAACCTCGAGGGGCTCGTTCCTGTTAGCGGCACATTTACCCGAAGATCAAACGCAGCGCGATAAAATATTGATGGCAATTATGGGTTCCGGTAACGATCTGGAAATTGACgggagtgct                                      >  1:436594/1‑136 (MQ=255)                                                                                                      tACCCTGCGTGAGGATGCGAGGTGGAACCTCGAGTGGCTCGTTCCTATGTGCTGAACATTTACCCGAAGATCAAACGCAGCGCGATAAAATATTGATGGCAATTATGGGTTCCGGTAACGATCTGGAAATTGACGGTATTggc                                      <  2:474038/143‑1 (MQ=255)                                                                                                         ccTGCGTGATGATGCGAGGTGGAACCTCGAGGGGCTCGTTCCTGTTAGCGGAACATTTACCCGAAGATCAAACGCAGCGCGATAAAATATTGATGGCAATTATGGGTTCCGGGAACGATCTGGAAATTGACGGTATTggcggc                                   >  2:931277/1‑143 (MQ=255)                                                                                                           tGCGTGATGATGCGAGGTGGAACCTCGAGGGGCTCGTTCCTGTTAGCGGAACATTTACCCGAAGATCAAACGCAGCGCGATAAAATATTGATGGCACTTATGGGTTCCGGTCCCCATCTGGAAATTACCGGTATTGGcggcgg                                 >  1:39405/1‑143 (MQ=255)                                                                                                              gTGAGGATGCGAGGTGGATCCTCGGGGGGCTCGTGCGTTTTAGCGTAACATTTACCCGAAGATCAAACGCAGCGTGTTAAAATATTGATGGCAATTATGGGTTCCGGTAACGATCTGGAAATTGACGGTATTGGCGGCGGTaa                              <  2:556041/143‑1 (MQ=255)                                                                                                               tgatgaGGCGAGGTGGAACCTCGAGGGGCTCGTTCCTGTTAGCGGAACATTTACCCGAAGATCAAACGCAGCGCGATAAAATATTGATGGCAATTATGGGTTCCGGTAACGATCTGGAAATTGACGGTATTGGCGGCGGTAAt                             <  1:763563/143‑1 (MQ=255)                                                                                                                gatgatGCGAGGTGGAACCGCGAGGGGCTCGTTCCTGTTAGCGGAACATTTACCCGAAGATCAAACGCAGCGCGATAAAATATTGATGGCAATTATGGGTTCCGGTAACGATCTGGAAATTGACGGTATTGGCGGCGGTAATc                            <  1:178457/143‑1 (MQ=255)                                                                                                                gatgatGCGAGGGGGAACCTCGAGGGGCTCGTTCCTGTTAGCGGAACATTTACCCGAAGATCAAACGCAGCGCGATAAAATATTGATGGCAATTATGGGTTCCGGTAACGATCTGGAAATTGACGGTATTGGCGGCGGTAATc                            <  1:1165507/143‑1 (MQ=255)                                                                                                                  tgatgCGAGGTGGAACCTCGAGGGGCTCGTTCCTGTTAGCGGAACATTTACCCGAAGATCAAACGCAGCGCGATAAGATATTGAGGGCAATTATGGGTTCCGGTAACGATCTGGAAATTGACGGTATTGGCGGCGGTAATCCa                          <  1:717943/143‑1 (MQ=255)                                                                                                                  tgaggCGAGGTGGAACCCCGAGGGGCTCGTTCCTGTTAGCGGAACATTTCCCCGAAGATCAATCGCAGCGCGATAAAATATTGATGGCAATTATGGGTTCCGGTAACGATCTGGAAATTGACGGTATTGGCGGCGGTAATCCa                          <  1:604137/143‑1 (MQ=255)                                                                                                                   gatgCGAGGTGGAACCTCGAGGGGCTCGTTCCTGTTAGCGGAACATTTACCCGAAGATCAAACGCAGCGCGATAAAATATTGATGGCAATTATGGGTTCCGGTAACGATCTGGAAATTGACGGTATTGGCGGCGGTAATCCAc                         <  1:705616/143‑1 (MQ=255)                                                                                                                    gtgCGAGGTGGCACCTCGAGGGGCTCGTTCCTGTTAGCGGAACATTTACCCGAAGATCAAACGCAGCGCGATAAACTATTGATGGCAATTATGGGTTCCGGTAACGATCTGGAAATTGACGGTATTGGCGGCGGTAATCCACt                        <  1:889036/142‑1 (MQ=255)                                                                                                                    atgCGAGGTGGAACCTCGAGGGGCTCGTTCCTGTTAGCGGAACATTTTCCCGAAGATCAAAAGCAGCGCGACAAAATATTGATGGCAATTATGGGTTCCGGTAACGATCTGGAAATTGACGGTCAGGGCGGCGGTAACCCCCt                        >  2:892467/1‑143 (MQ=255)                                                                                                                    atgCGAGGTGGAACCTCGAGGGGCTCGTTCCTGTTAGCGGAACATTTACCCGAAGATCAAACGCAGCGCGATAAAATATTGATGTCAATTATGGGTTCCGGTAACGATCTGGAAATTGACGGTATTGGCGGCGGTAATCCACt                        <  1:851362/143‑1 (MQ=255)                                                                                                                    atgCGAGGTGGAACCTCGAGGGGCTCGTTCCTGTTAGCGGAACATTTACCCGAAGAGCAAACGCAGCGCGATAAAATATTGATGGCAATTATGGGTTCCGGTAACGATCTGGAAATTGACGGTATTGGCGGCGGTAGTCCACt                        >  2:34372/1‑143 (MQ=255)                                                                                                                    atgCGAGGTGGAACCTCGAGGGGCTCGTGCCGGTTAGGGGAACATTTACCCTGATGTCAAACGCAGTGCTGTAAAATTTCGATGGCAATTATGGGTTCCGGTAACGATCTGGAAATTGACGGTATTGGCGGCGGTAATCCACt                        <  2:850507/143‑1 (MQ=255)                                                                                                                       cGAGGTGGAACCTCGAGGGGCTCGTTCCTGTTAGCGGAACATTTACCCGAAGATCAAACGCAGCGCGATAAAATATTGATGGCAATTATGGGTTCCGGTAACGATCTGGAACTTGACGGTATTGTCGTCGGTACTCCACTgcc                     >  1:1038886/1‑141 (MQ=255)                                                                                                                       cGAGCTGGAACCTCGAGGGGCTCGTTCCTGTTAGCGGAACATTTACCCGAAGATCAAACGCAGCGCGATAAAATATTGATGGCAATTATGGGTTCCGGTAACGATCTGGAAATTGACGGTATTGGCGGCGGTAATCCACTGAc                     <  2:743035/143‑1 (MQ=255)                                                                                                                       acaGGTGGAAACGCGGGGGGCTCGTAGCTGTTAGCGGAACATTTAACCGAAGATCAAACGCAGCGCGATAAAATATTGATGGCAATTATGGGTTCCGGTAACGAGCTGGAAATTGACGGTATTGGCGGCGGTAATCCACTGAc                     <  2:213525/141‑1 (MQ=255)                                                                                                                           gTGGAACCTCGAGGGGCTCGTTCCTGTTAGCGGAACATTTACCCGAAGATCAAACGCAGCGCGCTACAATATTGATGGCAATTATGGGTTCCGGTAACGATCTGGAAATTGACGGTATTGGCGGCGGTAATCCACTGACCAGt                 >  1:189998/1‑143 (MQ=255)                                                                                                                                   tCGAGGGGCTCGTTCCTGTTAGCGGAACATTTACCCGAAGATCAAACGCAGCGCGATAAAATATTGATGGCAATTATGGGTTCCGGTAACGATCTGGAAATTGACGGTATTGGCGGCGGTAACCCACTGACCAGTCACGTCGc         >  1:434967/1‑143 (MQ=255)                                                                                                                                    cGAGGGGCTCGTTCCTGTTAGCGGAACATTTACCCGAAGATCAAACGCAGCGCGATAAAATATTGATGGCAATTATGGGTTCCGGTAACGATCTGGAAATTGACGGTATTGGCGGCGGTAATCCACTGACCAGTAAAGTCGcc        >  2:994073/1‑143 (MQ=255)                                                                                                                                    cGAGGCGCTCGTTCCTGTTAGCGGAACATTTACCCGAAGATCAAACGCAGCGCGATAAAATATTGATGGCAATTATGGGTTCCGGTAACGATCTGGAAATTGACGGTATTGGCGGCGGTAATCCACTGACCAGTAAAGTCGcc        <  2:396527/143‑1 (MQ=255)                                                                                                                                          gcgcGTTCCTGTTAGCGGAACATTTACCCGAAGATCAAACGCAGCGCGATAAAATATTGATGGCAATTATGGGTTCCGGTAACGATCTGGAAATTGACGGTATTGGCGGCGGTAATCCACTGACCAGTAAAGTCGCCattatt  <  1:431390/143‑1 (MQ=255)                                                                                                                                            |                                                                                                                                             TATTAATCGTTGAGCTAAAGTCATTAGAGATGCTTTGCCCTTAATGTAACCATATCGCAATAAGTTATGTTTTTAAATTGAGGGCATTATTATGAAAAAAATACCCTGCGTGATGATGCGAGGTGGAACCTCGAGGGGCGCGTTCCTGTTAGCGGAACATTTACCCGAAGATCAAACGCAGCGCGATAAAATATTGATGGCAATTATGGGTTCCGGTAACGATCTGGAAATTGACGGTATTGGCGGCGGTAATCCACTGACCAGTAAAGTCGCCATTATT  >  NC_000913/800668‑800947

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑