Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,637,645:1 +T 100% coding (4/1500 nt) pitA → metal phosphate:H(+) symporter PitA

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,637,6451.T97.9% 143.1 / ‑3.4 47coding (4/1500 nt)pitAmetal phosphate:H(+) symporter PitA
Reads supporting (aligned to +/- strand):  ref base . (0/1);  new base T (17/29);  total (17/30)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.12e-01
Rejected as polymorphism: E-value score below prediction cutoff.
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

ATATAAGATAATTATTTTTTGAGTGAAATCCATACAGGGGGCAAATCAAAAAAAGTCTATATTTCACTTTGCCCGCGCCGCGAAAGTCACTGATAATGCGCCGCGTTCATGTCCTCAAAATGGCGTAACGTCCTATGC‑T‑ACATTTGTTTGCTGGCCTGGATTTGCATACCGGGCTGTTATTATTGCTTGCACTGGCTTTTGTGCTGTTCTACGAAGCCATCAATGGTTTCCATGACACAGCCAACGCCGTGGCAACCGTTATCTATACCCGCGC  >  NC_000913/3637508‑3637781
                                                                                                                                          |                                                                                                                                         
atataAGATCATTATTTTTTGCGTGAAATCCATCCAGGGGGCAAATCAAAAAAAGTCTATATTTCACTTTGCCCGCGCCGCGAAAGTCACTGATAATGCGCCGCGTTCATGTCCTCAAAATGGCGTAACGTCCTATGC‑ttaca                                                                                                                                      <  1:80999/143‑5 (MQ=255)
     aGATAATTATTTTTTGAGTGAAATCCATACAGGGGGCAAATCAAAAAAAGTCTATATTTCCCTTTGCCCGCGCCGCGAAAGTCACTGATAATGCGCCGCGTTCATGTCCTCAAAATGGCGTAACGTCCTATGCTT‑ACCtttgt                                                                                                                                 >  1:285591/1‑143 (MQ=255)
         aaTTATTTTTTGAGTGAAATCCATACAGGGGGCAAATCAAAAAAAGTCTATATTTCACTTTGCCCGCGCCGCGAAAGTCACTGATCATGCGCCGCGTTCATGTCCTCAAAATGGCGTAACGTCCTATGCTT‑ACATTTGTTTGc                                                                                                                             >  1:302552/1‑143 (MQ=255)
          gtcatggtttGGGTGAAAGCCAAACAGGGGGCAAAGCAAAAAAAGTCTATATTTCACTTTGCCCGCGCCGCGAAAGTCACTGATAATGCGCCGCGTTCATGTCCTCAAAATGGCGTAACGTCCTATGCTT‑ACATTTGTTTGCt                                                                                                                            <  2:52038/136‑1 (MQ=255)
             atatgttGAGTGAAATCCATACAGGGCGCACATCAACGCTATTCTATATTGCACTTTGCCCGCGCCGCGAAAGTCACTGATAATGCGCCGCGTTCATGTCCTCAAAATGGCGTAACGTCCTATGCTT‑ACATTTGTTTGCTGGc                                                                                                                         <  2:133617/138‑1 (MQ=255)
               tttttGAGTGAAATCCATACAGGGGGCAAATCAAAAAAAGTCTCTATTTCACTTTGCCCGCGCCGCGAAAGTCACTGATACTGCGCCGCGTTCATGTCCTCAAAATGGCGTAACGTCCTATGCTT‑ACATTTGTTTGCTGGCCt                                                                                                                       <  2:269008/143‑1 (MQ=255)
                             ccATACCGGGGGCAAATCAAAAAAAGTCTATATTTCACTTGGCCCGCGCCGGCAAAGTCACTGATAATGCGCCGCGTTCATGTCCTCAAAATGGCGTAACGTCCTATGCTT‑ACATTTGTTTGCTGGCCTGGATTTGCATACCg                                                                                                         <  1:628974/143‑1 (MQ=255)
                             ccATACAGGTGGCAAATCAAAAAAAGTCTATATCTCACTTTGCCCGCGCCGCGAAAGTCACTGATAATGCGCCGCGTTCATGTCCTCAAAATGGCGTAACGTCCTATGCTT‑ACATTTGTTTGCTGGCCTGGATTTGCATACCg                                                                                                         <  2:97992/143‑1 (MQ=255)
                               aTACAGGGGGCAAATCAAAAAAAGTCTATATTTCACTTTGCCCGCGCCGCGAAAGTCACTGATAATGCGCCGCGTTCATGTCCTCAAAATGGCGTCACGTCCTATGCTT‑ACGTTCGTTTGCTGGCCTGGATTTGAATACCggg                                                                                                       >  1:193548/1‑143 (MQ=255)
                                tACAGGGGGCAAATCAAAAAAAGTCTATATTTCACTTTGCCCGCGCCGCGAAAGTCACTGATAATGCGCCGCGTTCATGTCCTCAAAATGGCCTAACGTCCTATGCTT‑ACATTTGTTTGCTGGCCTGGATTTGCATACCGGGc                                                                                                      >  1:611840/1‑143 (MQ=255)
                                     ggggCAAATCAAAAAAAGTCTATATTTCACTTTGCCCGCGCCGCGAAAGTCACTGATAATGCGCCGCGTTCATGTCCTCAAAATGGCGTAACGTCCTATGCTT‑ACATTTGTTTGCTGGCCTGGCTTTGCCTCCCGGGCTGttc                                                                                                 >  2:428479/1‑142 (MQ=255)
                                              cAAAAGAAGTCTATATGTCACGTGGGCCGCGCCGCGAAAGTCACTGATAATGCGCCGCGTTCATGTCATCTAGATGTCGTGCTGTCCTACGCTT‑ACATTTGTTTGCTGGCCCGGATTGGGATACCGGGCTGTTATTAttgctt                                                                                        <  2:458405/143‑1 (MQ=255)
                                              cAAAAAAAGTCTATATTTCACTTTGCCCGCGCCGCGAAAGTCACTGATAATGCGCCGCGTTCATGTCCTCAAAATGGCGTAACGTCCTATGCTT‑ACATTTGTTTGCTGGCCTGGATTTGCATACCGGGCTGTTATTAttgctt                                                                                        <  1:215274/143‑1 (MQ=255)
                                              cAAAAAAAGTCTATATTTCACTTTGCCCGCGCCGCGAAAGTCACTGATAATGCGCCGCGTTCATGTCCTCAAAATGGCGTAACGTCCTATGCTT‑ACATTTGTTTGCTGGCCTGGATTTGCATACCGGGCTGTTATTAttgctt                                                                                        <  1:570750/143‑1 (MQ=255)
                                               aaaataaGTCTATATTGCACATTGCCCCCGCCGCGAAAGTCACTGATAATGCGCCGCGTTCATGTCCTCAAAATGGCGTAACGTCCTATGCTT‑ACATTTGTTTGCTGGCCTGGATTTGCATACCGGGCTGTTATTAttgcttg                                                                                       <  1:606538/143‑1 (MQ=255)
                                               aaaaaaaGTCTATATTTCACTTTGCCCGCGCCGCGAAAGTCACTGATAATGCGCCGCGTTCATGTCCTCAAAATGGCGTAACGTCCTATGCTT‑ACATTTGTTTGCTGGCCTGGATTTGCATACCGGGCTGTTATTAttgcttg                                                                                       <  1:610648/143‑1 (MQ=255)
                                                aaaaaaGTCTATATTTCACTTTGCCCGCGCCGCGAAAGTCACTGATAATGCGCCGCGTTCATGTCCTCAAAATGGCGTAACGTCCTATGCTT‑ACATTTGCTTGCTGGCCTGGATTTGCATACCGGGCTGTTATTAttgcttgc                                                                                      >  2:119580/1‑143 (MQ=255)
                                                   aaaGTCTATATTTCACTTTGCCCGCGCCGCGAAAGTCACTGATAATGCGCCGCGTTCATGTCCTCAAAATGGCGTAACGTCCTATGCTT‑ACATTTGTTTGCTGGCCTGGATTTGCATACCGGGCTGTTATTATTGCTTGCACt                                                                                   <  1:582418/143‑1 (MQ=255)
                                                    taGTCTACTTTTCGCTTGTCCCGCGCCGCGAAATTCACTTATCATGCGCCGCGTTCATGTCCTCAAAATGGCGTAACGTCCTATGCTT‑ACATTTGTTTGCTTGCCTGGATTTGCATACCGGGCTGTTATTATTGCTTGCACTg                                                                                  <  2:2496/142‑1 (MQ=255)
                                                    aaGTCTATATTTCACTTTGCCCGCGCCGCGAAAGTCACTGATAATGCGCCGCGTTCATGTCCTCAAAATGGCGTAACGTCCTATGCTT‑ACATTTGTTTGCTGGCCTGGATTTGCATACCGGGCTGTTATTATTGCTTGCACTg                                                                                  <  1:249629/143‑1 (MQ=255)
                                                          atatTTCACTTTGCCCTCGCCGCGAAAGTCACTGATAACGCGCCGCGTTCATGTCCTCAAAATGGCGTAACGTCCTATGCTT‑ACATTTGTTTGCTGGCCTGGATTTGCATACCGGGCTGTTATTATTGCTTGCACTGGCtttt                                                                            <  1:501180/143‑1 (MQ=255)
                                                          atatTTCACTTTGCCCGCGCCGCGAAAGTCACTGATAATGCGCCGCGTTCATGTCCTCAAAATGGCGTAACGTCCTATGCTT‑ACATTGGTTTGCTGGCCTGGATTTGCATACCGGGCTGGTAGTATTGGTTGCGCTgggtgtt                                                                            >  1:67785/1‑138 (MQ=255)
                                                           tatTTCACTTTGCCCGCGCCGCGAAAGTCACTGATAATGCGCCGCGTTCATGTCCTCAAAATGGCGTAACGTCCTATGCTT‑ACATTTGTTTGCTGGCCTGGATTTGCATACCGGGCTGTTATTATTGCTTGCACTGGCTTTtg                                                                           <  2:518624/143‑1 (MQ=255)
                                                             tttCACTTTGCCCGCGCCGCGAAAGTCACTGATAATGCGCCGCGTTCATGTCCTCAAAATGGCGTAACGTCCTATGCTT‑ACATTTGTTTGCTGGCCTGGATTTGCATACCGGGCTGTTATTATTGCTTGCACTGGCTTTtgtg                                                                         <  2:64975/143‑1 (MQ=255)
                                                             tttCACTTTGCCCGCGCCGCGAAAGTCACTGATAATGCGCCGCGTTCATGTCCTCAAAATGGCGTAACGTCCTATGCTT‑ACATTTGTTTGCTGGCCTGGATTTGCATACCGGGCTGTTATTATTGCTTGCACTGGCTTTtgtg                                                                         >  2:287268/1‑143 (MQ=255)
                                                               tCACTTTGCCCGCGCCGCGAAAGTCACTGATAATGCGCCGCGTTCATGTCCTCAAAATGGCGTAACGTCCTATGCTT‑ACATTTGTTTGCTGGCCTGGATTTGCATACCGGGCTGTTATTATTGCTTGCACTGGCTTTTGTGCt                                                                       <  1:65834/143‑1 (MQ=255)
                                                                  cTTTGCCCGCGCCGCGAAAGTCACTGATAATGCGCCGCGTTCATGTCCTCAAAATGGCGTAACGTCCTATGCTT‑ACATTTGTTTGCTGGCCTGGATTTGCATACCGGGCTGTTATTATTGCTTGCACTGGCTTTTGTGCTGtt                                                                    <  1:516116/143‑1 (MQ=255)
                                                                  cTTTGCCCGCGCCGCGAAAGTCACTGATAATGCGCCGCGGTCATGTCCTCAAAATGGCGTAACGTCCTATGCTT‑ACATTTGTTTGCTGGCCTGGATTTGCATACCGGGCTGTTATTATTGCTTGCACTGGCTTTTGTGCTGtt                                                                    <  2:3629/143‑1 (MQ=255)
                                                                           cgtcgcgAACGTCCCTGATAATGCGCCGCGTTCCTGTCCTCAAAATGGCGTAACGTCCTATGCTT‑ACATTTGTTTGGTGGCCTGGATTTGCATACCGGGCTGTTATTATTGCTTGCACTGGCTTTTGTGCTGTTCTACGAAGc                                                           <  2:638379/140‑1 (MQ=255)
                                                                                  aaaGTCACTGATAATGCGCCGCGTTCATGTCCTCAAAATGGCGTAACGTCCTATGCTT‑ACATTTGTTTGCTGGCCTGGATTTGCATACCGGGCTGTTATTATTGCTTGCACTGGCTTTTGTGCTGTTCTACGAAGCCATCAAt                                                    <  1:161007/143‑1 (MQ=255)
                                                                                   aaGTCACTGATAATGCGCCACGTTCATGTCCTCAAAATGGCGTAACGTCCTATGCTT‑ACATTTGTTTGCTGGCGTGGATTTGCATACCGAGCGGTGATTATTACTTGCACTCGCTTTTGTGCTGTTCTACGGATCCATcggtg                                                   >  1:173067/1‑139 (MQ=255)
                                                                                        aCTGATAATGCGCCGCGTTCATGTCCTCAAAATGGCGTAACGTCCTATGCTT‑ACATTTGTTTGCTGGCCTGGATTTGCATACCGGGCTGTGATTATTGCTTGCACTGGCTCTTGTGCTGTTCCGAGAAGCCATCAATgctctc                                              >  2:452192/1‑138 (MQ=255)
                                                                                          ttgtagtGCGTCGTGTTCATGTGCTGAAAATGGCTTAACGGTGTATGCTT‑ACATTTGTGTGCTTGCCTGGATTTGCATACCGGGCTGTTATTATTGCTTGCACTGGCTTTTGTGCTTTTCTATGAAGCCATCAATGGTTTCCa                                            <  2:246770/137‑1 (MQ=255)
                                                                                            cccaTGCGCCGCGTTCATGTCCTCAAAATGGCGTAACGTCCTATGCTT‑ACATTTGTTTGCTGGCCTGGATTTGCATACCGGGCTGTTATTATTGCTTGCACTGGCTTTTGTGCTGTTTTACGAAGCCATCAATGGTTTCCATg                                          <  1:476884/140‑1 (MQ=255)
                                                                                             tAATGCGCCGCGTTCATGTCCTCAAAATGGCGTAACGTCCTATGCTT‑ACATTTGTTTGCTGGCCTGGATTTGCATACCGGGCTGTTATTATTGCTTGCACTGGCTTTTGTGCTGTTCTACGAAGCCATCAATGGTTTCCATGa                                         <  1:484274/143‑1 (MQ=255)
                                                                                               aTGCGCCGCGTTCATGTCCTCAAAATGGCGTAACGTCCTATGCTT‑ACATTTGTTTGCTGGCCTGGATTTGCATACCGGGCTGTTATTATTGCTTGCACTGGCTTTTGTGCTGTTCTACGAAGCCATCAATGGTTTCCATGaca                                       <  2:103304/143‑1 (MQ=255)
                                                                                                tGCGCCGCGTTCATGTCCTCAAAATGGCGTAACGTCCTATGCTT‑ACATTTGTTTGCTGGCCTGGATTTGCATACCGGGCTGTTATTATTGCTTGCACTGGCTTTTGTGCTGTTCTACGAAGCCATCAATGGTTTCCATGacac                                      <  2:500601/143‑1 (MQ=255)
                                                                                                  cgccgcGTTCATGTCCTCAAAATGGCGTAACGTCCTGTGCTT‑ACATTTGTTTGCTGGCCTGGATTTGCCTACCCGGCTGTTATTATTGCTTGCACTGGCTTTTGTGCTGTTCTACGAAGCCGTCACTGGGTACCGTGacacgg                                    >  2:315106/1‑141 (MQ=255)
                                                                                                    ccgcGTTCATGTCCTCAAAATGGCGTAACGTCCTATGCTT‑ACATTTGTTTGCTGGCCTGGATTTGCATACCGGGCTGTTATGATTGCTTGCACTGGCTTTTGTGCTGTTCTACGAAGCCATCAATGGTTTCCCTAACACAGcc                                  >  2:6687/1‑143 (MQ=255)
                                                                                                          gcATGTCCTCAAAATGGCGTGCCGTCCTACGC‑TGACACTGGTTTGCTGGCCTGGAGTTGCTTGCCGGGCTGTTATTATTGCTTGCACTGGCTTTCTTGCTGTTCTACGAAGCCATCAATGGTTTCCATGACACAGCCAACGcc                            <  1:183458/142‑1 (MQ=255)
                                                                                                            ttGTCCTCAAAATGGCGTAACGTCCTATGCTT‑ACATTTGTTTGCTGGCCTGGATTTGCATACCGGGCTGTTATTATTGCTTGCACTGGCTTTTGTGCTGTTCTACGAAGCCATCTCTTTTTTCCATGACACAGCCAACGCCGt                          <  1:508615/142‑1 (MQ=255)
                                                                                                               tCCTCAAAATGGCGTAACGTCCTATGCTT‑ACATTTGTTTGCTGGCCTGGATTTGCATACCGGGCTGTTATTATTGCTTGCACTGGCTTTTGTGCTGTTCTACGAAGCCATCAATGGTTTCCATGACACAGCCAACGCCGTGGc                       <  1:263114/143‑1 (MQ=255)
                                                                                                                 cTCAAAATGGCGTAACGTCCTATGCTT‑ACATTTGTTTGCTGGCCTGGATTTGCATACCGGGCTGTTATTATTGCTTGCACTGGCTTTTGTGCTGTTCTACGAAGACATCAATGGTTTCCATGACACAGCCCACGCCGTGGCaa                     >  2:376528/1‑143 (MQ=255)
                                                                                                                      aaTGGCGTAACGTCCTATGCTT‑ACATTTGTTTGCTGGCCTGGATTTGCATACCGGGCTGTTATTATTGCTTGCACTGGCTTTTGTGCTGTTCTACGAAGCCATCAATGGTTTCCATGGAGCGGCCCACCCCGTGGCACCCGtt                >  2:417772/1‑143 (MQ=255)
                                                                                                                        tGGCGTAACGTCCTATGCTT‑ACATTTGTTTGCTGGCCTGGATTTGCATACCGGGCTGTTATTATTGCTTGCACTGGCTTTTGTGCTGTTCTACGAAGCCATCAATGGTTTCCATGACACAGCCAACGCCGTGGCAACCGTTAt              <  1:18867/143‑1 (MQ=255)
                                                                                                                                cGTCCTATGCTT‑ACATTTGTTTGCTGGCCTGGATTTGCATACCGGGCTGTTATTATTGCTTGCACTGGCTTTTGTGCTGTTCTACGAAGCCATCAATGGTTTCCATGACACAGCCAACGCCGTGGCAACCGTTATCTATAccc      >  1:234435/1‑143 (MQ=255)
                                                                                                                                  tCCTATGCTT‑ACATTTGTTTGCTGGCCTGGATTTGCATACCGGGCTGTTATTATTGCTTGCACTGGCTTTTGTGCTGTTCTACGAAGCCATCAATGGTTTCCATGACACAGCCAACGCCGTGGCAACCGTTATCTACACCcgc    >  2:384556/1‑143 (MQ=255)
                                                                                                                                    cTATGCTT‑ACATTTGTTTGCTGGCCTGGATTTGCATACCGGGCTGTTATTATTGCTTGCACTGGCTTTTGTGCTGTTCTACGAAGCCATCAATGGTTTCCATGACACAGCCAACGCCGCGGCAACCGTTATCTCTACCcgcgt  >  1:67887/1‑142 (MQ=255)
                                                                                                                                          |                                                                                                                                         
ATATAAGATAATTATTTTTTGAGTGAAATCCATACAGGGGGCAAATCAAAAAAAGTCTATATTTCACTTTGCCCGCGCCGCGAAAGTCACTGATAATGCGCCGCGTTCATGTCCTCAAAATGGCGTAACGTCCTATGC‑T‑ACATTTGTTTGCTGGCCTGGATTTGCATACCGGGCTGTTATTATTGCTTGCACTGGCTTTTGTGCTGTTCTACGAAGCCATCAATGGTTTCCATGACACAGCCAACGCCGTGGCAACCGTTATCTATACCCGCGC  >  NC_000913/3637508‑3637781

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑