| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_000913 | 125,387 | C→T | 100% | Q791* (CAG→TAG) | aceE → | pyruvate dehydrogenase E1 component |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 125,387 | 0 | C | T | 100.0% | 97.9 / ‑6.7 | 37 | Q791* (CAG→TAG) | aceE | pyruvate dehydrogenase E1 component |
| Reads supporting (aligned to +/- strand): ref base C (0/0); major base T (16/20); minor base G (0/1); total (16/21) | |||||||||||
| Rejected as polymorphism: E-value score below prediction cutoff. | |||||||||||
| Rejected as polymorphism: Frequency below/above cutoff threshold. | |||||||||||
| Rejected as polymorphism: Variant not supported by required number of reads on each strand. | |||||||||||
AGATCCTGGCGAAAGATTACGGCGTAGGTTCTGACGTTTATAGCGTGACCTCCTTCACCGAGCTGGCGCGTGATGGTCAGGATTGTGAACGCTGGAACATGCTGCACCCGCTGGAAACTCCGCGCGTTCCGTATATCGCTCAGGTGATGAACGACGCTCCGGCAGTGGCATCTACCGACTATATGAAACTGTTCGCTGAGCAGGTCCGTACTTACGTACCGGCTGACGACTACCGCGTACTGGGTACTGATGGCTTCGGTC > NC_000913/125247‑125507 | agaTCCTGGCGAAAGATTACGGCGTAGGTTCTGACGTTTATAGCGTGACCTCCTTCACCGAGCTGGCGCGTGATGGTCAGGATTGTGAACGCTGGAACATGCTGCACCCGCTGGAAACTCCGCGCGTTCCGTATATCGCTTAg < 1:103258/143‑1 (MQ=255) aTCCTGGCGAAAGATTACGGCGTAGGTTCTGACGTTTATAGCGTGACCTCCTTCACCGAGCTGGCGCGTGATGGTCAGGATTGTGAACGCTGGAACATGCTGCACCCGCTGGAAACTCCGCGCGTTCCGCACAACGCTcttgt < 2:261961/143‑5 (MQ=255) cgggCGAAATTTTTCGTCGTAGGTTCTGATGTTTATAGCGTGACCTCCTTCACCGGGCTGGCGCGTGATGGTCAGGATTGTGAACGCTGGAACATGCGGCACCCGCTGGAAACTCCGCGCGTTCCGTATATCGCTTAGGtgat < 1:346924/141‑1 (MQ=255) cTGGCGAAAGATTACGGCGTAGGTTCTGGCGTTTATAGCGTGACCTCCTTCACCGAGCTGGCGCGTGATGGTCAGGATTGTGAACGCTGGAACATGCTGCACCCGCTGGAAACTCCGCGCGTTCCGTATATCGCTTAGGtgat < 2:10975/143‑1 (MQ=255) cGAAAGATTACGGCGTAGGTTCTGACGTTTATAGCGTGACCTCCTTCACCGAGCTGGCGCGTGATGGTCAGGATTGTGAACGCTGGAACATGCTGCACCCGCTGGAAACTAAGCGCGATACGTATATAGCTTAGGAGATGAac > 1:201647/1‑143 (MQ=255) aaaGATTACGGCGTAGGTTCTGACGTTTATAGCGTGACCTCCTTCACCGAGCTGGCGCGTGATGGTCAGGATTGTGAACGCTGGAACATGCTGCACCCGCTGGAAACTCCGCGCGTTCCGTATATCGCTTAGGCGATGAacga > 2:287459/1‑143 (MQ=255) aaaGACCACGCCGTAGGTTCTGACGTTTATAGCGTGACCTCCTTCACCGAGCTGGCGCGTGATGGTCAGGATTGTGAACGCTGGAACATGCTGCACCCGCTGGAAACTCCGCGCGTTCCGTATATCGCTTAGGTGATGAacga < 1:292416/143‑1 (MQ=255) ttgcGGCGTAGGTTCTGACGTTTATAGCGTGAACTCCTTCACCGAGCTGGCGCGTGATGGTCAGGATTGTGAACGCTGGAACATGCTGCACCCGCTGGAAACTCCGCGCGTTCCGTATATCGCTTAGGTGATGAACGACGCTc < 1:15336/140‑1 (MQ=255) ttACGGCGGAGGTTCTGACGTTTATAGCATGAGCTCATTCACAGAGGTGGCGCGGGAGGGTGAGTAGTGGGAACTCTGGCACAGGCTGCACGCGGTGGAAACTCCGCGCGTACCGTATATCGCATAGGTGGTGAACGACGCta > 2:316330/1‑142 (MQ=255) cGGCGTGGGTGCGGACGTGTATAGCGTGACCTCCTTCACCGAGCTGGCGCGCGATGGTCAGGATTGTGAACGCTGGAACATGCTGCACCCGCTGGAAACGCCGCGCGTTCCGTATATCGCTTAGGTGATGAACGACGCTCCgg < 2:122271/143‑1 (MQ=255) cGGCGTAGGTTCTGACGTTTATAGCGTGACCTCCTTCACCGAGCTGGCGCGTGATGGTCAGGATTGTGAACGCTGGAACATGCTGCACCCGCTGGAAACTCCGCGCGTTCCGTATATCGCTTAGGTGATGAACGACGCTCCgg < 2:505236/143‑1 (MQ=255) gttgcTGACGTGTATAGCGTGGCCTCCTTCACCGAGCTGGCGCGTGATGGTCAGGATTGTGAACGCTGGAACATGCTGCACCCGCTGGAAACTCCGCGCGTTCCATATATCGCTTAGGTGATGAACGACGCTCCGGCAGTGGc < 2:194588/139‑1 (MQ=255) cggcGTTTATGGTGCGGCCTCCCACACCGAGCTGGCGCGTGATGGTCAGGAGTGTGAACGCTGGAACATGCTGCACCCGCTGGAAACTCCGCGCGTTCCGTCTACCGCTTAGGTGATGAACGACGCTCCGGCAGTGGCATCTa < 2:130609/140‑1 (MQ=255) gACGTTTATAGCGTGACCGCCTGCACCGAGCTGGCGCGTGATGGTCAGGATTGTGAACGCTGGAACATGCTGCACCCGCTGGAAACTCCGCGCGTTCCGTATATCGCTTAGGTGATGAACGACGCTCCGGCAGTGGCATCTAc < 2:581003/143‑1 (MQ=255) tatgGCGTGACGTCTCTCGGCGGGCTGGGCCGGGATGGTCAGGATTGTGAACGCTGGAACATGCTGCACGCGCTGGAAACTCCGCGCGTTCCGAATAACAAATCGAGAATAAACGACGCTCCGGCAGTGGCATCTACCGACta < 2:287957/143‑1 (MQ=255) tataGCGTGACCTCCTTCACCGAGCTGGCGCGTGATGGTCAGGATTGTGAACGCTGGAACATGCTGCACCCGCTGGAAACTCCGCGCGTTCCGTATATCGCTTAGGTGATGAACGACGCTCCGGCAGTGGCATCTACCGACta < 1:218368/143‑1 (MQ=255) aGCGTGACCTCCTTCACCGAGCTGGCGCGTGATGGTCAGGATTGTGAACGCTGGAACATGCTGCACCCGCTGGAAACTCCGCGCGTTCCGTATATCGCTTAGGTGATGAACGACGCTCCGGCAGTGGCATCTACCGACTatat < 1:513237/143‑1 (MQ=255) cccgcaCCTCCTTCCCCGACCTGGCGCGTCACGGTCAGGATTGTGCACGTTGGAACCTGCTGCACCCGCCGTAAACGCCGCGCGTTCCGTATATCGCTTAGGTGATGAACGACGCTCCGGCAGTGGCATCTACCGACTATATg < 1:262940/138‑1 (MQ=255) tGACCTCCTTCACCGAGCTGGCGCGTGATGGTCAGGAGTGTGAACGCTGGAACATGCTGCACCCGCTGGAAACTCCGCGCGTTCCGTATATCGCTTAGGAGATAAACGACGCTCCGGCAGTGGCCTCTACCGACTATATGaaa > 2:190698/1‑143 (MQ=255) cctcctTCACCGAGCTGGCGCGTGATGGTCAGGATTGTGAACGCTGGAACATGCTGCACCCGCTGGAAACTCCGCGCGTGCCGTATATCGCTTAGGTGATGAACGACGCTCCGGCAGTGGCATCTACCGACTATATGCAACTg > 1:270888/1‑143 (MQ=255) ctctTCACCTAGAGGACGCGTGATGGAAAGAATTTTGAACGCTGGAACATGCTGCACCCGCTGGAAACTCCGCGCGTTCCGAATAGCGCTGAGGTGATGAACCACGCTCCGGCAGTGGCATCTACCGACGATATGAAACtttt < 1:206350/141‑1 (MQ=255) ctTCACCGAGCTGGCGCGTGATGGTCAGGATTGTGAACGCTGGAACATGCTCCACCCGCTGGAATCTCCGCGCGTTCCGTATATCGCTTAGGTGATCAACGACGCTCCGGCAGTGGCATCTACCGACTATATGAAACTGTTCg < 1:541518/143‑1 (MQ=255) cGAGCTGGCGCGTGATGGTCAGGATTGTGAACGCTGGAACATGCTGCACCCGCTGGAAACTCCGCGCGTTCCGTATATCGCTTAGGTGATGAACGACGCTCCGGCAGTGGCATCTACCGACTATATGAGACTGGGCGCTGAGc > 2:65215/1‑143 (MQ=255) ggCGCGTGATGGTCAGGATTGTGAACGCTCGAACATGCTTCACCCGCTGGAAACTCCGCGCGTTCCGTATATCGCTTAGGTGATGAACGACGCTCCGGCAGTGGCATCTACCGACTATATGAAACTGTTCGCTGAGCAGGTcc < 2:21312/143‑1 (MQ=255) aGGATTGTGAACGCTGGAACATGCTGCACCCGCTGGAAACTCCGCGCGTTCCGTATATCGCTTAGGTGATGAACGACGCTCCGGCAGTGGCATCTACCGACTATATGAAACTGTTCGCTGAGCAGGTCCGTACTTACGTACCg > 2:293900/1‑143 (MQ=255) aGGATTGTGAACGCTGGAACATGCTGCACCCGCTGGAAACTCCGCGCGTTCCGTATATCGCTTAGGTGATGAACGACGCTCCGGCAGTGGCATCTACCGACTATACGAAACCGTTCGCTGAGCAGGTCCGTACTCAGGTACCg > 2:439508/1‑143 (MQ=255) gATTGTGAACGCTGGAACATGCTGCACCCGCTGGAAACTCCGCGCGTTCCGTATATCGCTTAGGTGATGAACGCCGCTCCGGCAGTGGCATCTACCGACTATATGAAACTGTTCGCTGAGCAGGTCCGTACTTACGTACCGGc > 1:454655/1‑143 (MQ=255) tGGAACTTCCTGCCCCCGTGGGCACCTCCGCGCGTTCCGTATATCGCTTAGGTGATGAACGACGTTCGGTCAGTGACATCTACCGACTATAGTAAACTGTTCGCTGAGCAGGTCCGTACTTACGTACCGGCTGACGACTACcg < 2:350802/143‑1 (MQ=255) cATGCTGCACCCGCTGGAAACTCCGCGCGTTCCGTATATCGCTTAGGTGATGAACGACGCTCCGGCAGTGGCATCTACCGACTATATGAAACTGTTCGCTGAGCGGGTCCGTACTTACGTACCGGCTGACGACTAGcgtctac > 2:341861/1‑138 (MQ=255) gtgcggcACCGGCTGGAACCTGCGGCGGGTCCGTATATCGCTTAGGTGAGGGACGACGCTCCGGCAGTGGCATCTACCGACTAGATGAAACTGTTCGCTGAGCAGGTCCGTACTTACGTACCGGCTGACGACTACCGCGTACt < 2:484157/142‑1 (MQ=255) gcACCCGCTGGAAACTCCGCGCGTTCCGTATATCGCTTAGGTGATGAACGACGCTCCGGCAGTGGCATCTACCGACTATATGAAACTGTTCGCTGAGCAGGTCCGTACTTACGTACCGGCTGACGACTACCGCGTACTGGGTa > 1:81837/1‑143 (MQ=255) ctcccGCCGGATACTCCGCGCGGTCCGTATATCGCTTAGGTGATGAACGACGCTCCGGCAGTGGCATCTACCGACTATATGAATCTGTTCGCTGAGCAGGTCCGTACTTACGTACCGGCTGACGACTACCGCGTACTGGGTAc < 2:469040/141‑1 (MQ=255) cACCCGCTGGAAACTCCGCGCGCTCCGTATATCGCTTAGGTGATGAACGACGCTCCGGCAGTGGCATCGACCGACGATATGAAACTGTCCGCGGAGCAGGTCCGTCATTACGAACAGGAGGCCGACAACCGCGTACGGGGCAc > 2:592946/1‑143 (MQ=255) cccGCTGGAAACTCCGCGCGTTCCGTATATCGCTTAGGTGATGAACGACGCTCCGGCAGTGACATCTACCGACTATATGAAACTGTTCGCTGAGCAGGTCCGTACTTACGTACCGGCTGACGACTACCGCGTACGGGGTACTg > 2:373542/1‑143 (MQ=255) cTGGAAACTCCGCGCGTGCCGTATATCGCTTAGGTGATGAGCGACGCTCCGGCAGTGGCATCTACCGACTATATGAAACTGTTCGCTGAGCAGGTCCGTACTTACGTACCGGCTGACGACTACCGCGTACTGGGTACTGATgg < 1:188162/143‑1 (MQ=255) aaCTCCGCGCGTTCCGTATATCGCTTAGGTGATGAACGACGCTCCGGCAGTGGCATCTACCGACTATATGAAACTGTTCGCTGAGCAGGTCCGTACTTACGTACCGGCTGACGACTACCGCGTACTGGGTACTGATGGCTTCg > 2:33325/1‑143 (MQ=255) aaCTCCGCGCGTTCCGTATATCGCTTAGGTGATGAACGACGCTCCGGCAGTGGCATCTAACGACTAGATGAAACTGTTCGCTGAGCAGGTCCGTACTTACGTACCGGCTGACGACTACCGCGTGCTGGGCAATGAAGACTTCg > 2:547014/1‑143 (MQ=255) tCCGCGCGTTCCGTATATCGCTTAGGTGATGAACGACGCTCCGGCAGTGGCATCTACCGACTATATGAAACTGTTCGCTGAGCAGGTCCGTACTTACGTACCGGCTGCCGACTACCGCGTACTGGGGAGTGATGGCTTCggcc > 1:292582/1‑141 (MQ=255) | AGATCCTGGCGAAAGATTACGGCGTAGGTTCTGACGTTTATAGCGTGACCTCCTTCACCGAGCTGGCGCGTGATGGTCAGGATTGTGAACGCTGGAACATGCTGCACCCGCTGGAAACTCCGCGCGTTCCGTATATCGCTCAGGTGATGAACGACGCTCCGGCAGTGGCATCTACCGACTATATGAAACTGTTCGCTGAGCAGGTCCGTACTTACGTACCGGCTGACGACTACCGCGTACTGGGTACTGATGGCTTCGGTC > NC_000913/125247‑125507 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |