| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_000913 | 759,250 | G→T | 100% | R182L (CGC→CTC) | sucA → | subunit of E1(0) component of 2‑oxoglutarate dehydrogenase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 759,250 | 0 | G | T | 100.0% | 71.7 / ‑6.7 | 28 | R182L (CGC→CTC) | sucA | subunit of E1(0) component of 2‑oxoglutarate dehydrogenase |
| Reads supporting (aligned to +/- strand): ref base G (0/1); new base T (13/13); total (14/14) | |||||||||||
| Rejected as polymorphism: E-value score below prediction cutoff. | |||||||||||
| Rejected as polymorphism: Frequency below/above cutoff threshold. | |||||||||||
| Rejected as polymorphism: Variant not supported by required number of reads on each strand. | |||||||||||
CTTCAACGTCGGTTCATTTGCCAGCGGCAAAGAAACCATGAAACTCGGCGAGCTGCTGGAAGCCCTCAAGCAAACCTACTGCGGCCCGATTGGTGCCGAGTATATGCACATTACCAGCACCGAAGAAAAACGCTGGATCCAACAGCGTATCGAGTCTGGTCGCGCGACTTTCAATAGCGAAGAGAAAAAACGCTTCTTAAGCGAACTGACCGCCGCTGAAGGTCTTGAACGTTACCTCGGCGCAAAATTCCCTGGCG > NC_000913/759119‑759375 | cTTCAACGTCGGTTCATTTGCCAGCGGCAAAGAAACCATGAAACTCGGCGAGCTGCTGGAAGCCCTCAAGCAAACCTACTGCGGCCCGGTTGGTGCCGAGGATATGCACATTACCAGCACGGCAGAAAAACCCTGGATcccag > 2:586970/1‑140 (MQ=255) tCGGTTCATTTGCCAGCGGCAAAGAAACCATGAAACTCGGCGAGCTGCTGGAAGCCCTCAAGCAAACCTACTGCGGCCCGATTGGTGCCGAGTATATGCACATTACCAGCACCGAAGAAAAACTCTGGATCCAACAGCGTATc < 2:604490/143‑1 (MQ=255) tgcttggCCAGCAGCAAAGAAGCCAGGAAACCCGGCGAGCTGCTGGAAGCCCTCAAGCAAACCTACTGCGGCCCGATTGGTGCCGAGTATCTGCACATTACCAGCACCGAAGAAAAACTCTGGATCCAACAGCGTATCGAGTc < 2:416220/137‑1 (MQ=255) tCATTTGCCAGCGTCAAACAATCCATGAACCTCTGCGAGCTGCTGGAAGCCCTCAAGCAAACCTACTGCTGCCCTATTGGTGCCTAGTATATGCACATTACCTGCACCGAAGAAATTCTCTGGATCCAACAGCGTATCGAGTc < 1:352670/143‑1 (MQ=255) cAGCGGCAAAGAAACCATGAAACTCGGCGAGCTGCTGGAAGCCCTCAAGCAAACCTACTGCGGCCCGATTGGTGCCGAGTATATGCACATTACCAGCACCGAAGAAAAACTCTGGATCCAACAGCGTATCGAGCCTGGTagcg > 1:279523/1‑143 (MQ=255) ggCAAAGAAACCGTGAAACTCGGCGAGTTGCTGGAAGCCCTCAAGCAAACCTACTGCGGCCCGATTGGTGCTGAGTATATGCACATTACCAGCACCGAAGAAAAACTCTGGATCCAACAGCGTATCGAGTCTGGTCGCGCGAc < 2:415752/143‑1 (MQ=255) ggCAAAGAAACCATGAAACTCGGCGAGCTGCTGGAAGCCCTCAAGCAAACCTACTGCGGCCCGATTGGTGCCGAGTATATGCACATTACCAGCACCGAAGAAAAACTCTGGATCCAACAGCGTATCGAGTCTGGTCGCGCGAc < 2:547331/143‑1 (MQ=255) cAAAGAAACCATGAAACTCGGCGAGCTGCTGGAAGCCCTCAAGCAAACCTACTGCGGCCCGCTTGGTCCCGAGTATATGCACATTACCAGCACCGCAGAAAAACTCTGGATCCACCCGCGTATCGAGTCTGGTCGCGCGACtt > 1:255327/1‑143 (MQ=255) gcatccATGCTACTCGGCGAGCTGCTGGAAGCCCTCCAGCACACCTACTCCGGCCCGAGTGGTGCCGAGTATATGCACATTACCAGCACCGAAGAAAAACTCTGGATCCAACAGCGTATCGAGTCTGGTCGCGCGACTTTCaa < 1:225273/139‑1 (MQ=255) aaaCCATGAAACTCGGCGAGCTGCTGGAAGCCCTCAAGCAAACCTACTGCGGCCCGATTGGTGCCGAGTATATGCACATTACCAGCACCGAAGAAAAACTCTGGATCCAACAGCGTATCGAGTCTGGTCGCGCGACTTTCAAt > 1:108455/1‑143 (MQ=255) aCCATGAAAGTCGGGGAGCTGCTGGAAGCCCTCAAGCAAACCTACTGCGGCCCGATTGGTGCCGAGTATATGCACATTACCAGCACCGAAGAAAAACTCTGGATCCAACAGCGTATCGAGTCTGGTCGCGCGACTTTCAATAg < 1:85741/143‑1 (MQ=255) tgcaaCTCGGCGAGCTGGTGGAAGCCCTCAAGCAAACCTACTGCGGCCCGATTGGTGCCGAGTATATGCACATGACCAGCACCGAAGAAAAACTCTGGATCCAACAGCGTATCGAGTCTGGTCGCGCGACTTTCAATAGCGaa < 1:630785/140‑1 (MQ=255) ccGGCGAGCTGCTAGACCCCCTCATGCAAACCTACTGCGGCCCGATTGGTGCCGAGTATATGCACATTCCCAGCACCGAAGAAACACTCTGGATCCAACAGCGTATCGAGTCTGGTCGCGCGACTTTCAATAGCGAAGAGaaa < 2:190082/142‑1 (MQ=255) ggCGAGCTGCTGGAAGCCCTCAAGCAAACCTACTGCGGCCCGATTGGTGCCGAGTATATGCACATTACCAGCACCGAAGAAAAACTCTGGATCCAACAGCGTATCGAGTCTGGTCGCGCGACTTTCAATAGCGAAGCGaaaaa > 1:400877/1‑143 (MQ=255) ggAAGCCCTCAAGCAAACCTACTGCGGCCCGATTGGTGCCGAGTATATGCACATTACCAGCACCGAAGAAAAACTCTGGATCCAACAGCGTATCGAGTCTAGTCGCGCGACTTTCAATAGCGAAGAGAAAAAACGCTTCTTaa > 1:54575/1‑143 (MQ=255) ggAAGCCCGCACGCAAACCTACTGCGGCCCGAGTGGTGCCGAGTATATGCACAGTACCAGCACCGAAGAAAAACTCTGGATCGAACAGAGTATCGAGTCGGGTCGCGCGACTTTCAATAGCGAAGAGAAAAAACGCTTCTTaa < 2:149269/143‑1 (MQ=255) aaGCCCTCAAGCAAACCTACTGCGGCCCGATGGGTGCCGAGTATATGCACATTACCAGCACCGAAGAAAAACTCTGGATCCAACAGCGTATCGAGTCTGGTCGCGCGACTTTCAATAGCGAAGAGAAAAAACGCTTCTTAAGc < 1:586111/143‑1 (MQ=255) aaCCTACTGCGGCCCGATTGGTGCCGAGTATATGCACATTACCAGCACCGAAGAAAAACTCTGGATCCAACAGCGTATCGAGTCTGGTCGCGCGACTTTCAAAAGCGAAGAGAAAACACGCAACTTAAACCAACTGAccgccg > 2:590522/1‑143 (MQ=255) aaCCTACTGCGGCCCGATTGGTGCCGAGTATATGCACATTACCAGCACCGAAGAAAAACTCTGGATCCAACAGCGTATCGAGTCGGGCCACGCGACTTTCAATAGCGAAGAGAAAAAACGCTTCTTAAGCGAACTGAccgccg > 1:471183/1‑143 (MQ=255) gCGGCCCGATTGGTGCCGAGTATATGCACATTACCAGCACCGAAGAAAAACTCTGGATCCAACAGCGCATCGAGTCTGGTCGCGCGACTTTCAATAGCGAACAGCAAACACGCTTCCTAAGCCAACTGACCGCCGCTGAAGGt > 2:277291/1‑143 (MQ=255) tgCCCGATTGGTGCCGAGTATATGCACATTACCAGCACCGAAGAAAAACTCTGGACCCAACAGCGTAACGAGACTGGTCGCGCGACTTTCAATAGCGAAGAGATAAAACGCTTCGTAAGCGAACTGACCGCCGCTGAAggccc > 2:617200/2‑140 (MQ=255) cGATTGGTGCCGAGTATATGCACATTACCAGCACCGAAGAAAAACTCTGGATCCAACAGCGTATCGAGTCTGGTCGCGCGACTTTCAATAGCGAAGAGAAAGAACGCTTCTTGAGCGAGCTGACCGCCGCTGAAGGTCTTGaa > 1:229906/1‑143 (MQ=255) gATGGGTGCCGATTATATGCACATTGCCGGCACCGAGGAAACACTCTGGATCCAACAGCGTAGCGAGTCTGGTCGCGCGACTTTCAATAGGGAAGAGAAAACACGCTTCTTAAGCGAACTGACCGCCGCTGAAGGTGTTGAAc < 2:263484/143‑1 (MQ=255) tGCCGAGTATATGCACATTACCAGCACCGAAGAAAAACTCTGGATCCAACAGCGTATCGAGTCTGGTCGCGCGACTTTCAATAGCGAAGAGAAAAAACGCTTCTTAAGCGAACTGACCGCCGCTGAAGGTCTTGAACGTTAcc > 1:401704/1‑143 (MQ=255) tatGCGCATTACCACCACCGAAGAAAAACGCTGGTGCCAACAGCGTATCGAGTCTGGTCGCGCGACTTTCAATAGCGAAGAGAAAAAACGCTTCTTAAGCGAACTGACCGCCGCTGAAGGTCTTGAACGTTACCTCGGCGCaa < 2:526264/143‑1 (MQ=255) cacaTTACCAGCACCGAAGAAAAACTCTGGATCCAACAGCGTATCGAGTCTGGTCGCGCGACTTTCAATAGCGAAGAGAAAAAACGCTTCTTAAGCGAACTGACCGCCGCTGAAGGTCTTGAACGTTACCTCGGCGCAAAAtt > 1:463632/1‑143 (MQ=255) ccAGCACCGAAGAAAAACTCTGGATCCAACAGCGTATCGAGTCTGGTCGCGCGACTTTCAATAGCGAAGAGAAAAAACGCTTCTTAAGCGAACTGACCGCCGCTGAAGGTCTTGAACGTTACCTCGGCGCAAAATTCCCTGgc < 2:274127/143‑1 (MQ=255) cAGCACCGAAGAAAAACTCTGGATCCAACAGCGTATCGAGTCTGGTCGCGCGACTTTCAATAGCGAAGAGAAAAAACGCTTCTTAAGCGAACTGACCGCCGCTGAAGGTCTTGAACGTTACCTCGGCGCAAAATTCCCTGgcc > 2:26865/1‑142 (MQ=255) | CTTCAACGTCGGTTCATTTGCCAGCGGCAAAGAAACCATGAAACTCGGCGAGCTGCTGGAAGCCCTCAAGCAAACCTACTGCGGCCCGATTGGTGCCGAGTATATGCACATTACCAGCACCGAAGAAAAACGCTGGATCCAACAGCGTATCGAGTCTGGTCGCGCGACTTTCAATAGCGAAGAGAAAAAACGCTTCTTAAGCGAACTGACCGCCGCTGAAGGTCTTGAACGTTACCTCGGCGCAAAATTCCCTGGCG > NC_000913/759119‑759375 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |