Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_000913	800,807	G→T	100%	A17S (GCG→TCG)	ybhH →	putative isomerase YbhH

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	800,807	0	G	T	100.0%	74.8 / NA	27	A17S (GCG→TCG)	ybhH	putative isomerase YbhH
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base T (10/17);  total (10/17)
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

TTAATCGTTGAGCTAAAGTCATTAGAGATGCTTTGCCCTTAATGTAACCAT‑ATCGCAATAAGTTATGTTTTTAAATTGAGGGCATTATTATGAAAAAAATACCCTGCGTGATGATGCGAGGTGGAACCTCGAGGGGCGCGTTCCTGTTAGCGGAACATTTACCCGAAGATCAAACGCAGCGCGATAAAATATTGATGGCAATTATGGGTTCCGGTAACGATCTGGAAATTGACGGTATTGGCGGCGGTAATCCACTGACCAGTAAAGTCGC  >  NC_000913/800670‑800940                                                                                                                                           |                                                                                                                                      ttaATCGTTGAGCTAAAGTCATTAGAGATGCTTTGCCCTTAAAG‑ATGCATGATCGCAATAAGTTATGTTTTTAAATTGAGGGCATTATTATGAAAAAAATACCCTGCGTGATGATGCGAGGTGGAACCTCGAGGGGCTCGTTc                                                                                                                                  <  1:612351/143‑1 (MQ=255)  taATCGTGAAGGTAAAGGCATGAGAGATTCGTTGGCGTTAATGTATACAT‑ATCGCAATAAGTGATGTGTTTAAAGTGAGGGCATTATGATGAAAAAAATACCCTGCGTGAGGATGCGAGGTGGAACCTCGAGGGGCTCGTTcc                                                                                                                                 <  2:468104/143‑1 (MQ=255)        ttGAGCTAAAGTCATTAGAGATGCTTTGCCCTTAATGTAAGCAT‑ATCGCAATAAGTTATGTTTTTAAATTGAGGGCATTATTAGGAAAAAAATACCCTGCGTGATGATGCGAGGTGGAAGCTCGAGGGGCTCGTCCCTGTTAg                                                                                                                           >  2:316519/1‑143 (MQ=255)         tGAGCTAAAGTCATTAGAGATGCTTTGCCCTTAATGTAACCAT‑ATCGCAATAAGTTATGTTTCTAAATTGAGGGCATTATTATGAAAAAAATACCCTGCGTGATGATGCGAGGTGGAACCTCGAGGGGCTCGTTCCTGTTAGc                                                                                                                          <  1:325949/143‑1 (MQ=255)            gCTAAAGTCATTAGAGATGCTTTGCCCTTAATGTAACCAT‑ATCGCAATAAGTTATGTTTTTAAATTGCGGGCATTATTATGAAAAAAATACCCTGCGTGATGATGCGAGGTGGAACCTCGAGGGGCTCGTTCCTGTTAGCGGa                                                                                                                       >  1:373394/1‑143 (MQ=255)                   tCATTAGAGATGCTTTGCCCTTAATGTAACCAT‑ATCGCAATAAGTTATGTTTTTAAATTGAGGGCATTATTATGAAAAAAATACCCTGCGTGATGATGCGAGGTGGAACCTCGAGGGGCTCGTTCCTGTTCGCGGACCAtttc                                                                                                                >  2:75669/1‑142 (MQ=255)                      ttAGAGATGCTTTGCCCTTAATGTAACCAT‑ATCGCAATAAGTTATGTTTTTAAATTGAGGGCATTATTATGAAAAAAATACCCTGCGTGATGATGCGAGGTGGAACCTCGAGGGGCTCGTTCCTGTTAGCGGAACATTTAccc                                                                                                             >  2:604625/1‑143 (MQ=255)                         gagaTTCTTTGACCTTAATGTAACCAT‑AGCGCAATAAGTTATGTTTTTAAATTGAGGGCATTATTATGAAAAAAATACCCTGCGTGATGATGCGAGGTGGAACCTCGAGGGGCTCGTTCCTGTTAGCGGAACATTTACCCGaa                                                                                                          <  2:394860/143‑1 (MQ=255)                                          aTGTAACCGT‑ATCGCAATAAGCTATGTTTTTAAATTGAGGGCAATAATAAAAAATAAAGACCCGGCGTGATGATGCGAGGTGGAACCTCGAGGGGCTCGTTCCTGTTAGCGGAACATTTACCCGAAGATCAAACGCAGCGCGa                                                                                         <  2:559624/143‑1 (MQ=255)                                            gTAACCAT‑ATCGCAATAAGTTATGTTTTTAAATTGAGGGCATTATTATGAAAAAAATACCCTGCGTGATGATGCGAGGTGGAACCTCGAGGGGCTCGTTCCTGTTAGCGGAACATTTACCCGAGGATTAAACGCCGTGCGATa                                                                                       >  1:292873/1‑143 (MQ=255)                                                       cGCAATAAGTTATGTTTTTAAATTGAGGGCATTATTATGAAAAAAATACCCTGCGTGATGATGCGAGGTGGAACCTCGAGGGGCTCGTTCCTGTTAGCGGAACATTTACCCGAAGATCAAACGCAGCGCGATAAAATATTGAt                                                                             <  1:113079/143‑1 (MQ=255)                                                         cAATAAGTTATGTTTTTAAATTGAGGGCATTATTATGACAAAAATACCCTGCGTGATGATGCGAGGTGGAACCTCGAGGGGCTCGTTCCTGTTAGCGGAACATTTACCCGAGGATCAGACGCAGCGCGATAAAGTATTGATgg                                                                           >  1:222245/1‑143 (MQ=255)                                                                           aaTTGAGGGCATTATTATGAAAAAAATACCCTGCGTGATGATGCGAGGTGGAACCTCGAGGGGCTCGTTCCTGTTAGCGGAACATTTACCCGAAGATCAAAAGCAGCGCGATAAAATATTGATGGCAATTATGGGTTCCGGTa                                                         >  1:436778/1‑143 (MQ=255)                                                                             ttGAGGGCATTATTATGAAAAAAATACCCTGCGTGATGATGCGAGGTGGAACCTCGAGGGGCTCGTTCCTGTTAGCGGAACATTTACCCGAAGATCAAACGCAGCGCGATAAAATATTGATGGCAATTATGGGTTCCGGTAAc                                                       <  2:557862/143‑1 (MQ=255)                                                                                 gggCATGATTATGGAAAAAATACCCTGCGTGATGATGCGAGGTGGAACCTCGAGGGGCTCGTTCCTGTTAGCGGAACATTTACCCGAAGATCAAACGCAGCGCGATAAAATATTGATGGCAATTATGGGTTCCGGTAACGATc                                                   <  2:23488/143‑1 (MQ=255)                                                                                      ttattaTGAAAAAAATACCCTGCGTGATGATGCGAGGTGGAACCTCGAGGGGCTCGTTCCTGTTAGCGGAACATTTACCCGAAGATCAAACGCAGCGCGATAAAATATTGATGGCAATTATGGGTTCCGGTAACGATCTGGaa                                              <  1:322076/143‑1 (MQ=255)                                                                                           atGAAAAAAATACCCTGCGTGATGATGCGAGGTGGAACCTCGAGGTGCTCGTTCCTGTTAACGGAACATTTACCCGAAGATCAAACGCAGCGCGATAAAATATTGATGGCAATTATGGGTTCCGGTAACGATCTGGAAATTGa                                         <  1:2369/143‑1 (MQ=255)                                                                                                aaaaaTACCCTGCGTGATGATGCGAGGTGGAACCTCGAGGGGCTCGTTCCTGTTAGCGGAACATTTACCCGAAGATCAAACGCAGCGCGATAAAATATTGATGGCAATTATGGGTTCCGGTAACGATCTGGAAATTGACGGTa                                    >  1:566466/1‑143 (MQ=255)                                                                                                         cTGCGTGATGATGCGAGGTGGAACCTCGAGGGGCTCGTTCCTGTTAGCGGAACATTTACCCGAAGATCAAACGCAGCGCGATAAAATATTGATAGCAATTATGGGTTCCGGTAACGATCTGGAAATTGACGGTATTGgcggcg                           <  2:628107/143‑1 (MQ=255)                                                                                                         cTGCGAAATGATGCGAGGTGGAAACCCGAGGGGCTCGTTCCTGTTAGCGGAACATTTACCCGAAGATCAAACGCAGCGCGATAAAATATTGATGGCAATTATGGGTTCCGGTAACGATCTGGAAATTGACGGTATTGgcggcg                           <  1:396554/143‑1 (MQ=255)                                                                                                                 tgaggCGAGGTGGAACCTCGAGGGGCTCGTTCCTGTTAGCGGAACATTTACCCGAAGATCAAACGCAGCGCGATAAAATATTGATGGCAATTATGGGTTCCGGTAACGATCTGGAAATTGACGGTATTGGCGGCGGTAATCCa                   <  2:212408/143‑1 (MQ=255)                                                                                                                  gatgCGAGGTGGAACCTCGAGGGGCTCGTTCCTGTTAGCGGAACATTTACCCGAAGATCAAACGCAGCGCGATAAAATATTGATGGCAATTATGGGTTCCGGTAACGATCTGGAAATTGACGGTATTGGCGGCGGTAATCCAc                  >  1:319377/1‑143 (MQ=255)                                                                                                                   gtggggggTGGAACCGCGGGGGGCTCGTTCCTGTTAGCGGAACATTTACCCGAAGATCAAACGCAGCGCGATAAAATATTGATGGCAATTATGGGTTCCGGTAACGATCTGGAAATTGACGGTATTGGCGGCGGTAATCCACt                 <  2:310983/137‑1 (MQ=255)                                                                                                                    tgCGAGGTGGAACCTCGAGGGGCTCGTTCCTGTTAGCGGAACATTTACCCGAAGATCAAACGCAGCGCGATAAAATATTGATGGCAATTATGGGTTCCGGTAACGATCTGGAAATTGACGGTATTGGCGGCGGTAATCCACTg                >  1:129239/1‑143 (MQ=255)                                                                                                                      cGATGTGGAACCTCGAGGGGCTCGTTCCTGTTAGCGGAACATTTACCCGAAGATCAAACGCAGCGCGATAAAATATTGATGGCAATTATGGGTTCCGGTAACGATCTGGAAATTGACGGTATTGGCGGCGGTAATCCACTGAc              <  1:237900/143‑1 (MQ=255)                                                                                                                             gAACCTCGAGGGGCTCGTTCCTGTTAGCGGAACATTTACCCGAAGATCAAACGCAGCGCGATAAAATATTGATGGCAATTATGGGTTCCGGTAACGATCTGGAAATTGACGGTATTGGCGGCGGTAATCCACTGACCAGTaaa       <  1:23391/143‑1 (MQ=255)                                                                                                                                  gcGAGGCGCTCGTGCCTGTTAGCGGAACATTTACACGAAGATCAAACGCAGCGCGATAAAATATTGATGGCAATTATGGGTTCCGGTAACGATCTGGAAATTGACGGTATTGGCGGCGGTAATCCACTGACCAGTAAAGTCGc  <  2:236980/142‑1 (MQ=255)                                                                                                                                           |                                                                                                                                      TTAATCGTTGAGCTAAAGTCATTAGAGATGCTTTGCCCTTAATGTAACCAT‑ATCGCAATAAGTTATGTTTTTAAATTGAGGGCATTATTATGAAAAAAATACCCTGCGTGATGATGCGAGGTGGAACCTCGAGGGGCGCGTTCCTGTTAGCGGAACATTTACCCGAAGATCAAACGCAGCGCGATAAAATATTGATGGCAATTATGGGTTCCGGTAACGATCTGGAAATTGACGGTATTGGCGGCGGTAATCCACTGACCAGTAAAGTCGC  >  NC_000913/800670‑800940

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑