| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_000913 | 1,388,284 | A→T | 14.7% | intergenic (+23/+21) | tyrR → / ← tpx | DNA‑binding transcriptional dual regulator TyrR/lipid hydroperoxide peroxidase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 1,388,284 | 0 | A | T | 14.7% | 90.5 / 11.8 | 41 | intergenic (+23/+21) | tyrR/tpx | DNA‑binding transcriptional dual regulator TyrR/lipid hydroperoxide peroxidase |
| Reads supporting (aligned to +/- strand): ref base A (20/15); new base T (2/4); total (22/19) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 3.90e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 2.50e-02 | |||||||||||
TTTATCGCAATTATCCCAGCACGCGCAAACTGGCAAAACGTCTCGGCGTTTCACATACCGCGATTGCCAATAAGTTGCGGGAATATGGTCTGAGTCAGAAGAAGAACGAAGAGTAAGCGCGAATATGCCTGATGGTGCAACACCATCAGGCATATTAAATTATGCTTTCAGTACAGCCAGAGCTGCTTCGTAATCCGGCTCGGTGGTGATTTCATCCACCAGCTGGCTGAAAATCACATTGTCATTTTCGTCAATAACCACAACGGCACGCGC > NC_000913/1388146‑1388418 | tttATCGCAATTATCCCAGCACGCGCAAGCTGGCAAAACGTCTCGGCGTTTCACATACCGCGATTGCCAATAAGTTGCGGGAATATGGTCTGAGTCAGAAGAAGAACGAAGAGTAAGCGCGAATATGCCTGATGGAGCAacgc > 2:618108/1‑141 (MQ=255)tgtATCGCAATTATCCCAGCACGCGGAAACTGGCAAAACGGCTCGGCGTTTCACATACCGCGATTGCCAATAAGTTGCGGGAATATGGTCTGAGTCAGAAGAAGAACGAAGAGTAAGCGCGAATATGCCTGATGGTGCAacac < 1:90213/141‑1 (MQ=255) tCGCAATTATCCCAGCACGCGCAAACTGGCAAAACGTCTCGGCGTTTCACATACCGCGATTGCCAATAAGTTGCGGGAATATGGTCTGAGTCAGAAGAAGAACGAAGAGTAAGCGCGAATATGCCAGATGGTGCAacaacctc > 2:549503/1‑138 (MQ=255) aaTTATTCCAGCACGCGCAAACTGGCAAAACGTCTCGGCGTTTCACATACCGCGATTGCCAATAAGTTGCGGGAATATGGTCTGAGTCAGAAGAAGAACGAAGAGTAAGCGCGAATATGCCTGATGGTGTTGCACCATCAGGc < 1:54091/143‑1 (MQ=255) aaTTAGTCCAGCAGGCGCAAACTGGCAAAACGTCTCGGCGTTTCACATACCGCGATTGCCAATAAGTTGCGCGAATATGGTCTGAGTCAGAAGAAGAACGAAGAGTAAGCGCGAATATGCCTGATGGTGTTGCACCATCAGGc < 2:244756/143‑1 (MQ=255) aTTCTCCCAGCGCGCGCAAACTGGCAAGACGTCTCGGCGTTTCACATACCGCGATTGCAAATAAGTTGCGGGAATATGGTCTGAGTCAGAAGAAGAACGAAGAGTAAGCGCGAATATGCCTGATGGTGCAACACCATCAGGCa < 2:482020/143‑1 (MQ=255) aaCTGGCAAAACGTCTCGGCGTTTCACATACCGCGATTGCCAATAAGTTGCGGGAATATGGTCTGAGTCAGAAGAAGAACGAAGAGTAAGCGCGAATATGCCTGATGGTGCAACACCATCAGGCATATTAAATTGTGCTTTCa > 1:73701/1‑143 (MQ=255) aCTGGCAAAACGGCTCGGCGTTTCACATACCGCGATTGCCAATAAGTTGCGGGAATATGGTCTGAGTCAGAAGAAGAACGAAGAGTAAGCGCGAATATGCCTGATGGTGCAACACCATCAGGCATATTAAATTATGCTTTCAg < 2:202838/143‑1 (MQ=255) tcAAAACGTTTCGTCGTTTCACATACCGCGATTGCCAATAAGTTGCGGGAATATGGTCTGAGTCAGCGGCGGCACGCAGAGTAAGCGCGAATATGCCTGATGGTGCAACACCATCAGGCATATTAAATTATGCTTTCAGTACa < 1:90721/142‑1 (MQ=255) gTCTCGGCGTGTCACATGCCGCGATTGCCAATAAGTTGCGGGAATATGGTCTGAGTCAGAAGAAGAACGAAGAGTAAGCGCGAATATGCCTGATGGTGCAACACCATCAGGCATATTAAATTATGCTTTCAGTACAGCCagag < 2:466644/143‑1 (MQ=255) tctcGGCGTTTCACATACCGCGATTGCCAATAAGTTGCGGGAATATGGTCTGAGTCAGAAGAAGAACGAAGAGTAAGCGCGAATATGCCTGATGGTGCAACACCATCAGGCATATTAAATTATGCTTTCAGTACAGCCAGAgc > 1:377528/1‑143 (MQ=255) aCCGCGATTGCCAATAAGTTGCGGGAATATGGTCTGAGTCAGAAGAAGAACGAAGAGTAAGCGCGAATATGCCTGATGGTGCAACACCATCAGGCATATTAAATTATGCTTTCAGTACAGCCAGAGCTGCTTCGTAATCCGGc > 1:614493/1‑143 (MQ=255) cAATAAGTTGCGGGAATATGGTCTGAGTCAGAAGAAGAACGAAGAGTAAGCGCGAATATGCCTGATGGTGTTGCACCATCAGGCATAttc > 1:500465/1‑89 (MQ=37) cAATAAGTTGCGGGAATATGGTCTGAGTCAGAAGAAGAACGAAGAGTAAGCGCGAATATGCCTGATGGTGTTGCACCATCAGGCATAttc > 1:500929/1‑89 (MQ=37) cAATAAGTTGCGGGAATATGGTCTGAGTCAGAAGAAGAACGAAGAGTAAGCGCGAATATGCCTGATGGTGTTGCACCATCAGGCATAttc < 2:500465/90‑2 (MQ=37) cAATAAGTTGCGGGAATATGGTCTGAGTCAGAAGAAGAACGAAGAGTAAGCGCGAATATGCCTGATGGTGTTGCACCATCAGGCATAttc < 2:500929/90‑2 (MQ=37) aaTAAGTTGCGGGAATATGGTCTGAGTCAGAAGAAGAACGAAGAGTAAGCGCGAATATGCCTGATGGTGCAACACCATCAGGCATATTAAATTATGCTTTCAGTACAGCCAGAGCTGCTTCGTAATCCGGCTCGGTGGTGAtt > 1:228400/1‑143 (MQ=255) tAAGTTGCGGGAATATGGTCTGAGTCAGAAGAAGAACGAAGAGTAAGCGCGAATATGCCTGATGGTGCAACACCATCAGGCATATTAAATTATGCTTTCAGTACAGCCAGAGCTGCTTCGTAATCCGGCTCGGTGGTGATTTc < 1:438757/143‑1 (MQ=255) gggAATATGGTCTGAGTCAGAAGAAGAACGAAGAGTAAGCGCGAATATGCCTGATGGTGCAACACCATCAGGCATATTAAATTATGCTTTCAGTACAGCCAGAGCTGCTTCGTAATCCGGCTCGGTGGTGATTTCATccacca > 1:324979/1‑143 (MQ=255) actatGGTCTGGGTCAGAAGAAGACCGAAGAGTAAGCGCGAATATGCCTGATGGTGCAACACCATCAGGCATATTAAATTATGCTTTCAGTACAGCCAGGGCTGCTTCGTAATCCGGCTCGGTGGTGATTTCATCCACCAgct < 2:127185/141‑1 (MQ=255) gTCTGAGTCAGAAGAAGAACGAAGAGTAAGCGCGAATATGCCTGATGGTGCAACACCATCAGGCATATTAAATTATGCATTCAGTACAGCCAGAGCTGCTTCGTAATCCGGCTCGGTGGTGATTTCATCCACCAGCTGGCTGa > 1:370051/1‑143 (MQ=255) tCTGAGTCAGAAGAAGAACGAAGAGTAAGCGCGAATATGCCTGATGGTGCAACACCATCAGACATCTTAAATTATGCTTTCAGTACAGCCGGAGCTGCTTCCTAATTAAGCGCGGTGGGCATTTCATCCACCCgctgcctcca > 2:216845/1‑140 (MQ=255) tCAGAAGAAGAACGAAGAGTAAGCGCGAATATGCCTGATGGTGCAACACCATCAGGCATATTAAATTATGCTTTCAGTACAGCCAGAGCTGCTTCGTAATCCGGCTCGGTGGTGATTTCATCCACCAGCTGGCTGAAAATcac < 1:92604/143‑1 (MQ=255) tCAGAAGAAGAACGAAGAGTAAGCGCGAATATGCCTGATGGTGCAACACCATCAGGCATATTAAATTATGCTTTCAGTACAGCCAGAGCTGCTTCGTAATCCGGCTCGGCGGTGATTCCATCACCCGACCAGCTGAAAATccc > 2:623566/1‑141 (MQ=255) aagaagaaCGAAGAGTAAGCGCGAATATGCCTGATGGTGCAACACCATCAGGCATATTAAATTATGCTTTGAGTACAGCCAGAGCTGCTTCGTAATCCGGCTAGGTGGTGATTTCATCCACCAGCTGGCTGAAAATCACATTg > 2:384531/1‑143 (MQ=255) aagaagaaCGAAGAGTAAGCGCGAATATGCCTGATGGTGCAACACCATCAGGCATATTAAATTATGCTTTCAGTACAGCCAGAGCTGCTTCGTAATCCGGCTCGGTGGTGATTTCATCCACCAGCTAGCTGAAAATCACATTg > 2:413429/1‑143 (MQ=255) cGAAGAGTAAGCGCGAATATGCCTGATGGTGCAACACCATCAGGCATATTAAATTATGCTTTCAGTACAGCCAGAGCTGCTTCGTAATCCGGCTCGGTGGTGATTTCATccacc < 2:353779/114‑1 (MQ=255) cGAAGAGTAAGCGCGAATATGCCTGATGGTGCAACACCATCAGGCATATTAAATTATGCTTTCAGTACAGCCAGAGCTGCTTCGTAATCCGGCTCGGTGGTGATTTCATccacc > 1:353779/1‑114 (MQ=255) gAAGAGTAAGCGCGAATATGCCTGATGGTGCAACACCATCAGGCATATTAAATTATGCTTTCAGTAAAGCCAGAGCTGCTTCGTAATCCGGCTCGGCGGCGATGTCTTCCGCCCCCTGGCTGAGGTCCACATTGTCATTTTcc > 1:270024/1‑142 (MQ=255) cagagTAAGCGCGGATATGCCTCACGGTGCAACGCCATCAGGCATATTAAGTTATGCTTGCAGTACAGCCAGAGCTGCTTCGTAATCCGGCTCGGTGGTGATTTCATCCACCAGCTGGCTGAAAATCACATTGTCATTTTCGt < 2:594845/142‑1 (MQ=255) aaGAGTAAGCGCGAATATGCCTGATGGTGCAACACCATCAGGCATATTAAATTATGCTTTCAGTACAGCCAGAGCTGCTTCGTAATCCGGCTCGGTGGTGATTTCATCCACCAGCTGGCTGAAAATCACATTGTCATTTTCGt < 1:234368/143‑1 (MQ=255) agagTAAGCGCGAATATGCCTGATGGTGCAACACCATCAGGCATATTAAATTATGCTTTCAGTACAGCCAGAGCTGCTTCGTAATCCGGCTCGGTGGTGATTTCATCCACCAGCTGGCTGAAAATCACATTGTCATTTTCGTc > 2:514451/1‑143 (MQ=255) agagGAAGCGCGAATATGCCTGACGGTGCAACACCATCAGGCATATTAAATTATGCTTTCAGTACAGCCAGAACAGCTTCTTAATCCGGCTCGGTGGTGATTTCATCCACCAGCTGGCTGAAAATCACATTGTCATTTTCGTc < 1:578869/143‑1 (MQ=255) tatgcgcGAATATGCTTGATGTTTCAGCACCCTCCGGCATGTTAATTTATGCTTTCAGTCCAGCCAGAGCTGCTTCTTAATCCGGCTCGTTGGTGATTTCATCCACCAGCTGGCTGAAAATCACATTGTCATTTTCGTCATTa < 2:494213/140‑1 (MQ=255) aaGCGCGAATATGCCTGATGGTGCAACACCATCAGGCATATTAAATTATGCTTTCAGTACAGCCAGAGCTGCTTCGTAACCCGGCTAGGTCGTCCGACCAGCCACCAGCTGGCTGAAGATCACATTTTCATTTTCGTCAATaa > 1:329754/1‑143 (MQ=255) aGCGCGAGTATGCCTAATGGTGCAACACCACCAGGCATATTAAATCATGCTTTCAGTACAGCCAGAGCTGCTTCGTAATCCGGCTCGGTGGTGATTTCATCCACCAGCTGGCTGAAAATCACATTGTCATTTTCGTCAATAAc < 1:198345/143‑1 (MQ=255) gAAGATGCCTGATGGTGCAACGCCATCAGGCATATTAAATTATGCTTCCAGTACAGCCAGAGCTGCTTCGTAAACCTGCGCGGTGGTGATTTCATCCACCAGCTGGCTGAAAATCACATTGTCATTTTCGTCAATAACCACaa < 1:90657/143‑1 (MQ=255) ccTGATGGTGCAACACCATCAGGCATATTAAATTATGCTTTCAGTACAGCCAGAGCTGCTTCGTAATCCGGCTCGGTGGTGATTTCATCCACCAGCTGGCTGAAAATCACCTTGTCATTTTCGTCAATAACCACACCGGcccc > 1:102113/1‑140 (MQ=255) tGATGGTGCAACACCATCAGGCATATTAAATTATGCTTTCAGTACAGCCAGAGCTGCTTCGTAATCCGGCTCGGTGGTGATTTCATCCACCAGCTGGCTGAAAATCACATTGTCATTTTCGTCAATAACCACAACGGCAcgcg > 1:518778/1‑143 (MQ=255) tGATGGTGCAACACCATCAGGCATATTAAATTATGCTTTCAGTACAGCCAGAGCTGCTTCGTAATCCGGCTCGGTGGTGATTTCATCCACCAGCTGGCTGAAAATCACATTGTCATGTTCGTCGATGACAACAACGGCAggcg > 2:577610/1‑143 (MQ=255) gATGGTGCAACACCATCAGGCATATTAAATTATGCTTTCAGTACAGCCAGAGCTGCTTCGTAATCCGGCTCGGTGGTGATTTCATCCACCAGCTGGCTGAAAATCACATTGTCATTTTCGTCAATAACCACAACGGCACgcgc > 2:528798/1‑143 (MQ=255) | TTTATCGCAATTATCCCAGCACGCGCAAACTGGCAAAACGTCTCGGCGTTTCACATACCGCGATTGCCAATAAGTTGCGGGAATATGGTCTGAGTCAGAAGAAGAACGAAGAGTAAGCGCGAATATGCCTGATGGTGCAACACCATCAGGCATATTAAATTATGCTTTCAGTACAGCCAGAGCTGCTTCGTAATCCGGCTCGGTGGTGATTTCATCCACCAGCTGGCTGAAAATCACATTGTCATTTTCGTCAATAACCACAACGGCACGCGC > NC_000913/1388146‑1388418 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |