| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_000913 | 2,132,787 | A→C | 100% | I204S (ATC→AGC) | wcaA ← | putative colanic acid biosynthesis glycosyl transferase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 2,132,787 | 0 | A | C | 99.9% | 67.6 / ‑7.7 | 25 | I204S (ATC→AGC) | wcaA | putative colanic acid biosynthesis glycosyl transferase |
| Reads supporting (aligned to +/- strand): ref base A (0/0); major base C (12/12); minor base G (0/1); total (12/13) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.97e-01 | |||||||||||
| Rejected as polymorphism: E-value score below prediction cutoff. | |||||||||||
| Rejected as polymorphism: Frequency below/above cutoff threshold. | |||||||||||
| Rejected as polymorphism: Variant not supported by required number of reads on each strand. | |||||||||||
TGGTAGAGGGTAAACAGCTGATATTTTTTGCTGGCGCGGTCGAATTTGTCTTTGTGCTTGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTG‑ATTGATATGCAGGATCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGAAGATGTCGTAATCCTGTGCCGCTTTCAGTTCGGTATCGAACAAACACTCTTTGAAACGCCATGCCCAGGTAAAGACCTG > NC_000913/2132652‑2132923 | tGGTAGAGGGTAAACAGCTGATATTTTTTGCTGGCGCGGTCGAATTTGTCTTTGAGCTTGCGGTAAAAGTGGAAGTACCCGGAGACTTCTTTCGGTGACGAGGTGCTCTGCCCTTCACCGTG‑CTTGCTATGCAGGCTCTGCgg > 1:336067/1‑142 (MQ=255) ggTAAACAGCTGATATTTTTTGCTGGCGCGGTCGAATTTGTCTTTGTGCTTGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTG‑ATTGATATGCAGGCTCTGCGTCGCCtctt > 1:482255/1‑143 (MQ=255) tGATATTTGTTGCTGGCGCGGTCGAATTTGTCTTTGTGCTTGCGGTAAAGGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTG‑ATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCa < 2:296185/143‑1 (MQ=255) tttGCTGGCGCGGTCGAATTTGTCTTTGTGCTTGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTG‑ATTGATATGCGGGCTGTGCGTCGGCGCGTCTACTTTCCACGGTTCGc > 1:46223/1‑143 (MQ=255) tttGCTGGCGCGGTCGAATTTGTCTTTGTGCTTGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTTCAGTGACGAGGTGATCTGCATTTCACCGGG‑ATTGCTATGCAGGCTCCGCGTCGCCTCCTCTCCTCTCCCCGGATCGc > 2:195569/1‑143 (MQ=255) tGGCGCGGTCGAATTTGTCTTTGTGCTTGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTG‑ATTGATCTGCAGGCTCTGCGTCGCCTCTTCCACTTTCCACGGTTCGCCGTAc > 2:290320/1‑143 (MQ=255) gtgtgCGGGCGGGAAACGTGGGAGTAACCGGAGAATTTTTTCGGTGACGAGGTGATCTGCAGTTCACCGTG‑ATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGaa < 2:296529/142‑1 (MQ=255) gTAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTG‑ATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGAAGACGTCGTCAt > 1:279168/1‑143 (MQ=255) gTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTG‑ATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGAAGATGTCGTAATCCtgtg > 1:247335/1‑143 (MQ=255) ggAGAAGTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTG‑ATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGAAGATGTCGTAATCCTGTGCCGCTTTCAGtt < 1:174739/143‑1 (MQ=255) ttttttCGGTGACGAGGTGATCTGCATTTCACCGTG‑ATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGAAGATGTCGTAATCCTGTGCCGCTTTCAGTTCGGTAt < 1:379993/143‑1 (MQ=255) tttttCGGTGACGTGGTGATCTGCATGTCACAGTGCAATG‑TTTGTTTTGCCTGGGTCGCCTCGTCTACTTTCCACGGTTCGCCGTACTCCACCACCGTCCGCAGGAAGATGTCGTAATCCTGTGCCGCTTTCAGTTCGGTATc < 2:282895/143‑1 (MQ=255) ttttCGGTGACGAGGTGATCTGCATTTCACCGTG‑ATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGAAGATGTCGTAATCCTGTGCCGCTTTCAGTTCGGTATCg < 1:316776/143‑1 (MQ=255) ttttCGGTGACGAGGTGATCTGCATTTCACCGTG‑ATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGAAGAAGTCGTAATCCTGTGCCGCTTTCAGTTCGGTATCg > 2:313915/1‑143 (MQ=255) ttCGGTGCCGAGGTGATCTGCCTTTCACTGTG‑ATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGAAGATGTCGTAATCCTGTGCCGCTTTCAGTTCGGTATCGaa < 2:363339/143‑1 (MQ=255) ggTGACGAGGTGATCTGCATTTCACCGTG‑ATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGAAGATGTCGTAATCCTATGCCGCTGTCAGACCGGTATCGaacaa > 2:208708/1‑143 (MQ=255) gTGACGAGGTGATCTGCATTTCACCGTG‑ATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGAAGATGTCGTAATCCTGTGCCGCTTTCAGTTCGGTATCGaacaaa > 1:344354/1‑143 (MQ=255) gTGACGAGGTGATCTGCATTTCACCGTG‑ATTGATATGCAGGCTCTGCGGCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCGACCACCATCCGCAGGAAGATGTCGTAATCCTGTGCCGCTTTCAGTTCGGTATCGaacaag > 1:36095/1‑142 (MQ=255) aCGAGGTGATCTGCATTTCACCGAG‑ATGGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCCCCATCCGCAGGAAGATGTCGTAATCCTGTGCCGCTTTCAGTTCGGTATCGAACAAacac < 2:281435/143‑1 (MQ=255) tctcCATTTCACCGGT‑ATTGTTATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGAAGATGTCGTAATCCTGTGCCGCTTTCAGTTCGGTATCGAACAAACACTCTTTGaaa < 2:362345/143‑1 (MQ=255) cATTTCACCGTG‑ATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGAAGATGTCGTAATCCTGTGCCGCTTTCAGTTCGGTATCGAACAAACACTCTTTGAAACGcc < 1:222362/143‑1 (MQ=255) aCCGTG‑ATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGGACTCCACCACCATCCGCAGGAAGATGTCGTAATCCTGTGCCGCTTTCAGTTCGGTATCGAACAAACACTCTTTGAAACGCCATGccc < 2:482255/143‑1 (MQ=255) gtgctttGCATGCTCTGCGTCGCCTCTTCTACTTTCCACGGTCCGCCGTGCTCCACCACCTTCCGCAGGAAGATGTCGTAATCCTGTGCCGCTTTCAGTTCGGTATCGAACAAACACTCTTTGAAACGCCATGCCCAGGTaaa < 2:441050/137‑1 (MQ=255) ctgatATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGAAGATGTCGTAATCCTGTGCCGCTTTCAGTTCGGTATCGAACAAACACTCTTTGAAACGCCATGCCCAGGTaaa < 1:32756/142‑1 (MQ=255) tGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGAAGATGTCGTAATCCTGTGCCGCTTTCAGTTCGGTATCGAACAAACACTCTTTGAAACGCCATGCCCAGGTAAAGACCTg > 1:198892/1‑143 (MQ=255) | TGGTAGAGGGTAAACAGCTGATATTTTTTGCTGGCGCGGTCGAATTTGTCTTTGTGCTTGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTG‑ATTGATATGCAGGATCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGAAGATGTCGTAATCCTGTGCCGCTTTCAGTTCGGTATCGAACAAACACTCTTTGAAACGCCATGCCCAGGTAAAGACCTG > NC_000913/2132652‑2132923 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |