| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_000913 | 2,484,369 | A→G | 10.2% | *205* (TAA→TAG) | evgA → | DNA‑binding transcriptional activator EvgA |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 2,484,369 | 0 | A | G | 10.2% | 104.5 / 3.0 | 44 | *205* (TAA→TAG) | evgA | DNA‑binding transcriptional activator EvgA |
| Reads supporting (aligned to +/- strand): ref base A (23/16); new base G (2/3); total (25/19) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 6.38e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.98e-01 | |||||||||||
CATTGCTGAAAAAATGTTCATCAGCAACAAAACTGTCAGCACTTATAAAAGTCGCCTGATGGAAAAATTAGAATGTAAATCACTGATGGATCTTTACACATTCGCACAACGTAACAAAATCGGCTAACCACATGAAGTTTTTACCCTATATTTTTCTTCTCTGTTGTGGTCTTTGGTCGACCATAAGTTTCGCAGACGAAGATTACATCGAATATCGTGGCATCAGTAGTAACAACCGTGTCACACTTGATCCACTACGTCTGAGCAAC > NC_000913/2484243‑2484511 | cATTGCTGAAAAAATGTTCATCAGCAACAAAACTGTCAGAACTTATAAAAGTCGCCTGATGGAAAAATTAGAATGTAAATCACTGATGGATCTTTACACATTCGCACAACGTAACAAAATCGGCTAACCACATGAAGTTTTTa < 1:250276/143‑1 (MQ=255) tGAAAAAATGTTCATCAGCAACAAAACTGTCAGCACTTATAAAAGTCGCCTGATGGAAAAATTAGAATGTAAATCACTGATGGATCTTTACACATTCGCACAACGTAACAAAATCGGCTAACCACATGAAGTTTTTACCCtct > 1:38400/1‑141 (MQ=255) tGAAAAAATGTTCATCAGCAACAAAACTGTCAGCACTTATAAAAGTCGCCTGATGGAAAAATTAGAATGTAAATCACTGATGGATCTTTACACATTCGCACAACGTAACAAAATCGGCTAACCACATGAAGTTTTTACCCtat > 1:414905/1‑143 (MQ=255) aaTGTTCATCAGCAACAAAACTGTCAGCACTTATAAAAGTCGCCTGATGGAAAAATTAGAATGTAAATCACTGATGGATCTTTACACATTCGCACAACGTAACAAAATCGGCTAGCCACATGAAGTTTTTACCCTATAttttt > 1:41334/1‑143 (MQ=255) aaTGTTCATCAGCAACAAAACTGTCAGCACTTATAAAAGTCGCCTGATGGAAAAATTAGAATGTAAATCACTGATGGATCTTTACACATTCGCACAACGTAACAAAATCGGCTAGCCACATGAAGTTTATACCCTATAttttt > 1:41313/1‑143 (MQ=255) tcatcaGCAACAAAACTGTCAGCACTTATAAAAGTCGCCTGATGGAAAAATTAGAATGTAAATAACTGATGGATATTTACACATTCGCACAACGTAACAAAATCGGCTAACCACATGAAGTTTGTACACTATATTTttctttt > 1:282107/1‑141 (MQ=255) catcaGCAACAAAACTGTCAGCACTTATAAAAGTCGCCTGATGGAAAAATTAGAATGTAAATCACTGATGGATCTTTACACATTCGCACAACGTAACAAAATCGGCTAGCCACATGAAGTTTTTACCCTATATTTTTCTtctc < 1:198634/143‑1 (MQ=255) atcaGCAACAAAACTGTCAGCACTTATAAAAGTCGCCTGATGGAAAAATTAGAATGTAAATCACTGATGGATCTTTACACATTCGCACAACGTAACAAAATCGGCTAACCACATGAGGTTTTTACCCTATATTTttcttacca > 2:587492/1‑139 (MQ=255) gtaacaaAACTGCCAGCACTTATAAAAGTCGCCTGATGCAAAAATTAGAATGTAAATCACTGATGGATCTTTACACATTCGCACAACGTAACAAAATCGGCTAACCACATGAAGTTTTTACCCTATATTTTTCTTCTCtgttg < 1:498918/141‑1 (MQ=255) aacaaAACTATCAGCACTTAGAAAAGGCGCCTGATGGAAAAATTAGAATGTAAATCACTGATGGATCTTTACACATTCGCACAACGTAACAAAATCGGCTAACCACATGAAGTTTTTACCCTATATTTTTCTTCTCTGTtgtg < 2:344543/143‑1 (MQ=255) aaaaCTGTCAGCACTTATAAAAGTCGCCTGATGGAAAAATTAGAATGTAAATCACTGATGGATCTTTACACATTCGCACAACGTAACAAAATCGGCTAACCACATGAAGTTTTTACCCTACATTTTTCTTCTCTGTTGTGGTc > 1:552598/1‑143 (MQ=255) cTGTCAGCACTTATAAAAGTCGCCTGATGGAAAAATTAGAATGTAAATCACTGATGGATCTTTACATATTCGCACAACGTAACAAAATCGGCTAACCACATGAAGTTTTTACCCTATATTTTTCTTCTCTGTTGTGGTCTTTg < 2:406701/143‑1 (MQ=255) aGCACTTATAAAAGTCGCCTGATGGAAAAATTAGAATGTAAATCACTGATGGATCTTTACACATTCGCACAACGTAACAAAATCGGCTAACCACATGAAGTTTTTACCCTATATTTTTCTTCACTGTTGTGGTCTTTGGTCGa > 2:175793/1‑143 (MQ=255) gCACTTATAAAAGTCGCCTGATGGAAAAATTAGAATGTAAATCACTGATGGATCTTTACACATTCGCACAACGTAACAAAATCGGCTAACCACATGAAGTTTTTACACTCTATTTTTCTTCTCTCTTGTGGTCTTTGGTcatc > 2:632681/1‑140 (MQ=255) aCTTATAAAAGTCGCCTGATGGAAAAATTAGAATGTAAATCACTGATGGATCTTTACACATTCGCACAACGTAACAAAATCGGCTAACCACATGAAGTTTTTACCCTATATTTTTCTTCTCTGTTGTGTTCTTTGGTCGACCa > 1:382148/1‑143 (MQ=255) ttATAAAAGTCGCCTGATGGAAAAATTAGAATGTAAATCACTGATGGATCTTTACACATTCGCACAAGGTAACAAAATCGGCTAACCACATAAAGTTTTTAACCTATATTCTTCTTCTCTGTTGTGGTCGTTGGACGACCATa > 1:604759/1‑143 (MQ=255) taAAAGTCGCCTGATGGAAAAATTAGAATGTAAATCACTGATGGATCTTTACACATTCGCACAACGTAACAAAATCGGCTAACCACATGAAGTTTTTACCCTATATTTTTCTTCTCTGTTGTGGTCTTTGGTCGACCATAAGt > 2:644930/1‑143 (MQ=255) aaGTCGCCTGATGGAAAAATTAGAATGTAAATCACTGATGGATCTTTACACATTCGCACAACGTAACAAAATCGGCTAACCACATGAAGTTTTTACCCTATATTTTTCTTCTCTGTTGTGGTCTTTGGTCGACCATAAGTTTc > 1:256402/1‑143 (MQ=255) aGTCGCCTGATGGAAAAATTAGAATGTCAATCACATCAAGAGGTTGACACATTCGCACAACGTAACAAAATCGGCTAACCACATGAAGTTTTTACCCTATATTTTTCTTCTCTGTTGTGGTCTTTGGTCGACCATAAGTTTCg < 1:117774/143‑1 (MQ=255) cGCCTGATGGAAAAATTAGAATGTAAATCACTGATGGATCTTTACACATTCGCACAACGTAACAAAATCGGCTAACCACATGAAGTTTTTACCCTATATTTTTCTTCTCTGTTGTGGTCTTTGGTCGACCATAAGTTTCGCAg < 1:246682/143‑1 (MQ=255) ccTGATGGAAAAATTAGAATGTAAATCACTGATGGATCTTTACACATTCGCACAACGTAACAAAATCGGCTAACCACATGAAGTTTTTACCCTATATTTTTCTTCTCTGTTGTGGTCTTTGGTCGACCATAAGTTTCGCAGAc > 2:632272/1‑143 (MQ=255) tGATGGAAAAATTAGAATGTAAATCACTGATGGATCTTTACACATTCGCACAACGTAACAAAATCGGCTAACCACATGAAGTTTTTACCCTATATTTTTCTTCTCTGTTGTGGTCTTTGGTCGACCATAAGTTTCGCAGACGa > 2:578747/1‑143 (MQ=255) gATGGAAAAATTAGAATGTAAATCACTGATGGATCTTTACACATTCGCACAACGTAACAAAATCGGCTAACCACATGAAGTTTTTACCCTATATTTTTCTTCTCTGTTGTGGTCTTTGGTCGACCATAAGTTTCGCAGACGaa < 1:161954/143‑1 (MQ=255) aTGGAAAAATTAGAATGTAAATCACTGATGGATCTTTACACATTCGCACAACGTAACAAAATCGGCTAACCACATGAAGTTTTTACCCTATATTTTTCTTCTCTGTTGTGGTCTTTGGTCGACCATAAGTTTCGCAGACGAAg < 1:420037/143‑1 (MQ=255) tGGAAAAATTAGAATGTAAATCACTGATGGATCTTTACACATTCGCACAACGTAACAAAATCGGCTAACCACATGAAGTTTTTACCCTATATTTTTCTTCTCTGTTGTGGTCTTTGGTCGACCATAAGTTTCGCAGACGAAGa < 1:511709/143‑1 (MQ=255) gAAAAATTAGAATGTAAATCACTGATGGATCTTTACACATTCGCACAACGTAACAAAATCGGCTAACCACATGAAGTTTTTACCCTATATTTTTCTTCTCTGTTGTGGTCTTTGGTCGATCATAAGTTTCGCAGTCTAAGagt > 2:413333/1‑141 (MQ=255) gAAAAATTAGAATGTAAATCACTGATGGATCTTTACACATTCGCACAACGTAACAAAATCGGCTAACCACATGAAGTTTTTACCCTATATTTTTCTTCTCTGTTGTGGTCTTTGGTCGACCATAAGTTTCGCAGCCGAAGact > 1:189512/1‑141 (MQ=255) gAAAAATTAGAATGTAAATCACTGATGGATCTTTACACATTCGCACAACGTAACAAAATCGGCTAACCACATGAAGTTTTTACCCTATATTTTTCTTCTCTGTTGTGGTCTTTGGTCGACCATAAGTTTCGCAGACGAAGAtt > 1:546742/1‑143 (MQ=255) ttAGAATGTAAATCACTGATGGATCTTTACACATTCGCACAACGTAACAAAATCGGCTAACCACATGACGTTTTTACCCTATATTTTTCTTCTCTGCTGTGGTCTTTGGTCGACAATAAGTTTCGCAGACGACGATTACGTcc > 2:577651/1‑142 (MQ=255) tAAATCACTGATGGATCTTTACACATTCGCACAACGTAACAAAATCGGCTAACCACATGAAGTTTTTACCCTATATTTTTCTTCTCTGTTGTGGTCTTTGGTCGACCATAAGTTTCGCAGACGAAGATTACATCGAATATCGt < 1:351112/143‑1 (MQ=255) aaaTCTCTGCTAGATGATTACACTTTCGCACAACGTAACAAAATCGGCTAACCACATGAAGTTTTTACCCTATATTTTTCTTCTCTGTTGTGGTCTTTGGTCGACCATAAGTTTCGCAGACGAAGATTACATCGAATATCGTg < 2:414905/143‑1 (MQ=255) aaaTCACTGATGGATCTTTACACATTCGCACAACGTAACAAAATCGGCTAACCACATGAAGTTTTTACCCTATATTTTTCTTCTCTGTTGTAGTCTTTGGTCGACCATAAGTTTCGCAGACGAAGATTACATCGAATATCGTg < 1:277210/143‑1 (MQ=255) aTGGATCTTTACACATTCGCACAACGTAACAAAATCGGCTAGCCACATGAAGTTTTTACCCTATATTTTTCTTCTCTGTTGTGGTCTTTGGTCGACCATAAGTTTCGCAGACAAAGATTACATCGAATATCGTGGCATCagta < 2:41334/143‑1 (MQ=255) aTGGATCTTTACACATTCGCACAACGTAACAAAATCGGCTAGCCACATGAAGTTTATACCCTATATTTTTCTTCTCTGTTGTGGTCTTTGGTCGACCATAAGTTTCGCAGACAAAGATTACATCGAATATCGTGGCATCagta < 2:41313/143‑1 (MQ=255) gATCTTTACACATTCGCACAACGTAACAAAATCGGCTAACCACATGAAGTTTTTACCCTATATTTTTCTTCTCTGTTGTGGTCTTTGGTCGACCATAAGTTTCGCAGACGAAGATTACATCGAATATCGTGGCATCAgtagta > 1:581086/1‑143 (MQ=255) tttACACATTCGCACAACGTAACAAAATCGGCTAACCACATGAAGTTTTTACCCTATATTTTTCTTCTCTGTTGTGGTCTTTGGTCGACCATAAGTTTCGCAGACGAAGATTACATCGAATATCGTGGCATCAGTAGTgacaa > 2:388039/1‑143 (MQ=255) tttACACATTCGCACAACGTAACAAAATCGGCTAACCACATGAAGTTTTTACCCTATATTTTTCTTCTCTGTTGTGGTCTTTGGTCGACCATAAGTTTCGCAGACGAAGATTACATCGAATATCGTGGCATCAGTAGTaacaa < 1:213725/143‑1 (MQ=255) cacaTTCGCACAACGTAACAAAATCGGCTAACCACATGAAGTTTTTACCCTATATTTTTCTTCTCTGTTGTGGTCTTTGCTCGACCATAAGTTTCGCAGCCGAAGATTACATCGAATATCGTGGCATCAGTAGTaagagtcgt > 2:30468/1‑136 (MQ=255) cacaTCGTAACAACATCGGCTAACCACATGAAGTTTTTACCCTATATTTTTCTTCTCTGTTGTGGTCTTTGGTCGACCATAAGTTTCGCAGACGAAGATTACATCGAATATCGTGGCATCAGTAGTAACAACCGTGTCACACt < 2:291127/143‑1 (MQ=255) aaCGTAACAAAATCGGCTAACCACATGAAGTTTTTACCCTATATTTTTCTTCTCTGTTGTGGACTGTGGTCGACGATAAGTTTCGCAGACGAAGATTACATCGAATATCGTGGCATCAGTAGTAAGAATAGGGTCACACttca > 2:63918/1‑141 (MQ=255) aCGTAACAAAATCGGCTAACCACATGAAGTTTTTACCCTATATTTTTCTTCTCTGTTGTGGTCTTTGGTCGACCATAAGTTTCGCAGACGAAGATTACATCGAATATCGTGGCATCAGTAGTAACCACCGTGTAACGCtgcat > 2:68636/1‑139 (MQ=255) aaaaTCGGCTAACCACATGAAGTTTTTACCCTATATTTTTCTTCTCTGTTGTGGTCTTTGGTCGACCATAAGTTTCGCAGACGAAGATTACATCGAATATCGTGGCATCAGTAGTAACAACCGTGTCACACTTGATCCACTAc < 1:632272/143‑1 (MQ=255) tCGGCTAACCACATGAAGTTTTTACCCTATATTTTTCTTCTCTGTTGTGGTCTTTGGTCGACCATAAGTTTCGCAGACGAAGATTACATCGAATATCGTGGCATCAGTAGTAACAACCGTGTCACACTTGATCCACTACGTCt < 1:134399/143‑1 (MQ=255) gCTAACCACATGAAGTTTTTACCCTATATTTTTCTTCTCTGTTGTGGTCTTTGGTCGACCAGAAGGTTGGCAGACGACAATTACATCGAATATCGTGGCATCAGTAGTAAAAACCGTGCCACAATTGATCCACTACGTCTGAg > 1:504766/1‑143 (MQ=255) aCCACATGAAGTTTTTACCCTATATTTTTCTTCTCTGTTGTGGTCTTTGGTCGACCATAAGTTTCGCAGACGAAGATTACATCGAATATCGTGGCATCAGTAGTAACAACCGTGTCACACTTGATCCACTACGTCTGAGcaac > 2:292859/1‑143 (MQ=255) | CATTGCTGAAAAAATGTTCATCAGCAACAAAACTGTCAGCACTTATAAAAGTCGCCTGATGGAAAAATTAGAATGTAAATCACTGATGGATCTTTACACATTCGCACAACGTAACAAAATCGGCTAACCACATGAAGTTTTTACCCTATATTTTTCTTCTCTGTTGTGGTCTTTGGTCGACCATAAGTTTCGCAGACGAAGATTACATCGAATATCGTGGCATCAGTAGTAACAACCGTGTCACACTTGATCCACTACGTCTGAGCAAC > NC_000913/2484243‑2484511 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |