| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_000913 | 2,568,161 | T→C | 13.0% | intergenic (‑54/+163) | eutH ← / ← eutG | putative inner membrane protein/putative alcohol dehydrogenase in ethanolamine utilization |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 2,568,161 | 0 | T | C | 13.0% | 97.5 / 6.7 | 45 | intergenic (‑54/+163) | eutH/eutG | putative inner membrane protein/putative alcohol dehydrogenase in ethanolamine utilization |
| Reads supporting (aligned to +/- strand): ref base T (20/18); new base C (2/4); total (22/23) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 6.64e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.65e-01 | |||||||||||
AACCGCCGAACTGCGACAGGATCCTGTCTACGGCAGCTATCAGCATAAAGAACATCATGATGTACATGATGATTTCGTTAATTCCCATCGCCTTTACTCCCTGTTAGTTGTTATTTATTGGCGGATGCGGCGTAAACGCCTTATCCGCCCTACATGTGCAATCCCGTAGGCTGGATAAGACGCGGCAAGCGTCGCATCCGGCAATTGCACCGCGCCACTGGCGGATGCGGCGTGAACGCCTTATCCGCCCTACATGTGCAATCCCGTAGGCTGGATAAGATGCG > NC_000913/2568020‑2568303 | aaCCGCCGAACTGCGACAGGATCCTGTCTACGGCAGCTCTCAGCATAAAGAACATCATGACGTACATGATGATTTCGTTAATTCCCATCGCCTTTACTCCCCGTTAGTTTTTATTTATTCGCGGATGCGCCGTAACCGCCTTa > 2:236261/1‑143 (MQ=255) ccgAACTGCGACAGGATCCTGTCTACGGCAGCTATCAGCATAAAGAACATCATGATGTACATGATGATTTCGTTAATTCCCATCGCCTTTACTCCCTGTTAGTTGTTATTTATTGGCGGATAAGGCGTTTACGCCGCATCCGc < 2:364887/143‑1 (MQ=255) cgAACTGCGACAGCATCCTGTCTACGGCAGCTATCAGCATAAAGAACATCATGATGTACATGATGATTTCGTTAATTCCCATCGCCTTTACTCCCTGTTAGTTGTTATTTATTGGCGGATAAGGCGTTTACGCCGCATCCGcc < 1:408949/143‑1 (MQ=255) aaCTGCGACAGGATCCTGTCTACGGCAGCTCTCAGCGTAAAGAACATCATGATGTACATGATGATTTCGTTAATGCCCATCGCCTTTACTCCCTGTTAGTTGTTATTTATTGGCGGATAAGGCGTTTACGCCGCATCCGccaa < 2:489502/143‑3 (MQ=255) caCAGGATCCTGTCTACGGCAGCTATCAGCATAAAGAACATCATGATGTACATGATGATTTCGTTAATTCCCATCGCCTTTACTCCCTGTTAGTTGTTATTTATTGGCGGATGCGGCGTAAACGCCTTATCCGCCCTACAtgt < 1:218722/142‑1 (MQ=255) aCAGGATCCTGTCTACGGCAGCTATCAGCATAAAGAACATCATGATGTACATGATGATTTCGTTAATTCCCATCGCCTTTACTCCCTGTTAGTTGTTATTTATTGGCGGATGCGGCGTAAACGCCTTATCCGCCCTACAtctg > 2:534362/1‑143 (MQ=255) ttCTACGGCAGCTATCAGCATAAAGAACATCATGATGTACATGATGATTTCGTTAATTCCCATCGCCTTTACTCCCTGTTAGTTGTTATTTATTGGCGGATGCGGCGTAAACGCCTTATCCGCCCTACATGTGCAATCCCGTa < 1:279113/142‑1 (MQ=255) gTCGACGGCAGCTATCAGCATAAAGAACATCATGATGTACATGATGATTTCGTTAATTCCCATCGCCTTTACTCCCTGTTAGTTGTTATTTATTGGCGGATGCGGCGTAAACGCCTTATCCGCCCTACATGTGCAATCCCGTa < 2:241555/143‑1 (MQ=255) gCATAAAGAACATCATGATGTACATGATGATTTCGTTAATTCCCATCGCCTTTACTCCCTGTTAGTTGTTATTTATTGGCGGATGCGGCGTAAACGCCTTATCCGCACTACATGTGCAATCCCGTAGGCTGGATAAGACGCgg > 1:86630/1‑143 (MQ=255) cATAAAGAACATCATGATGTACATGATGATTTCGTTAATTCCCATCGCCTTTACTCCCTGTTAGTTGTTATTTATTGGCGGATGCGGCGTAAACGCCTTATCCGCCCTACATGTGCAATCCCGTAGGCTGGATAAGACGCGGc > 1:311129/1‑143 (MQ=255) aTAAAGAACATCATGATGTACATGATGATTTCGTTAATTCCCATCGCCTTTACTCCCTGTTAGTTGTTATTTATTGGCGGATGCGGCGTAAACGCCTTATCCGCCCTACATGTGCAATCCCGTAGGCTGGATAAGACGCGGCa > 2:211285/1‑143 (MQ=255) aaaGAACATCATGATGTACATGATGATTTCGTTAATTCCCATCGCCTTTACTCCCTGTTAGTTGTTATTTATTGGCGGATGCGGCGTAAACGCCTTATCCGCCCTACATGTGCAATCCCGTAGGCTGGATAAGACGCGGCAAg > 2:197128/1‑143 (MQ=255) gAACAGCATGATGTACATGATGATTTCGTTAATTCCCATCGCCTTTACTCCCTGTTAGTTGTTATTTATTGGCGGATGCGGCGTAAACGCCTTATCCGCCCTACATGTGCAATCCCGTAGGCTGGATAAGACGCGGCAAGCGt < 1:104113/143‑1 (MQ=255) aCATCATGATGTACATGATGATTTCGTTAATTCCCAGCGCCTTTACTCCCTGTTAGTTGTTATTTATTGGCGGATGCGGCGTAAACGCCTTATCCGCCCTACATGTGCAATCCCGTAGGCTGGATAAGACGCGGCAAGCGTCg < 2:473207/143‑1 (MQ=255) atcatGATGTACATGATGATTTCGTTAATTCTCATCGCCTTTAATAAAAAATAAATGTAAATTATTGGCGGATGAGGCGTAAACGCCTTATCCGCCCTACATGTGCAATCCCGTATGCTGGATAAGCCGCGGCAAGCGTcaca > 1:138087/1‑143 (MQ=18) atcatGATGTACATGATGATTTCGTTAATTCCCATCGCCTTTACTCCCTGTTAGTTGTTATTTATTGGCGGATGCGGCGTAAACGCCTTATCCGCCCTACATGTGCAATCCCGTAGGCTGGATAAGACGCGGCAAGCGTCGCa > 2:123282/1‑143 (MQ=255) tcatGATGTACATGATGATTTCGTTAATTCCCATCGCCTTTACTCCCTGTTAGTTGTTATTTATTGGCGGATGCGGCGTAAACGCCTTATCCGCCCTACATGTGCAATCCCGTAGGCTGGATAAGACGCGGCAAGCGTCGCAt > 1:99954/1‑143 (MQ=255) atgatgTACATGATGATTTCGTTAATTCCCATCGCCTTTACTCCCTGTTAGTTGTTATTTATTGGCGGATGCGGCGTAAACGCATTATCCGCCCTACATGTGCAATCCCGTAGGCTGGATAAGACGCGGCAAGCGTCGCATcc < 1:135480/143‑1 (MQ=255) gatgTACATGATGATTTCGTTAATTCCCATCGCCTTTACTCCCTGTTAGTTGTTATTTATTGGCGGATGCGGCGTAAACGCCTTATCCGCCCTACATGTGCAATCCCGTAGGCTGGATAAGACGCGGCAAGCGTCGCATCCgg > 1:627298/1‑143 (MQ=255) gTACATGATGATTTCGTTAATTCCCATCGCCTTTACTCCCTGTTAGTTGTTATTTATTGGCGGATGCGGCGTAAACGCCTTATCCGCCCTACATGTGCAATCCCGTAGGCTGGATAAGACGCGGCAAGCGTCGCATCCGGCaa < 1:603911/143‑1 (MQ=255) gTACATGATGATTTCGTTAATTCCCATCGCCGTTGCTCCCTGTTAGTTGTTATTTATTGGCGGATGCGGCGTAAACGCCTTATCCGCCCTACATGTGCAATCCCGTAGGCTGGATAAGACGCGGCAAGCGTCGCATCCGGCaa < 2:222524/143‑1 (MQ=255) aCATGATGATTTCGTTAATTCCCATCGCCTTTACTCCCTGTTAGTTGTTATTTATTGGCGGATGCGGCGTAGACGCCTTATCCGCCCTACATGTGCAATCCCGTAGGCTGGATAAGACGCGGCAAGCGTCGCATCCGGCAAtt < 1:578855/143‑1 (MQ=255) atTTCGTTAATTCCCATCGCCTTTACTCCCTGTTAGTTGTTATTTATTGGCGGATGCGGCGTAAACGCCTTATCCGCCCTACATGTGCAATCCCTTAGGCTGGATAAGACGCGGACAGAGTTGCATCCGGCAGTCGCATcgcg > 1:158083/1‑143 (MQ=25) tttCGTTAATTCCCATCGCCTTTACTCCCTGTTAGTTGTTATTTATTGGCGGATGCGGCGTAAACGCCTTATCCGCCCTACATGTGCAATCCCGTAGGCTGGATAAGACGCGGCAAGCGTCGCATCCGGCAATTGCACCgcgc < 1:142816/143‑1 (MQ=255) ttCGTTAATTCCCATCGCCTTTACTCCCTGTTAGTTGTTATTTATTGGCGGATGCGGCGTACACGCCTTATCCGCCCTACATGTGCAATCCCGTAGGCTGGATAAGGCGCGGCAAGCGTCGCATCCGGCAATTGCACCGCGcc > 1:551268/1‑143 (MQ=255) ttAATTCCCATCGCCTTTACTCCCTGTTAGTTGTTATTTATTGGCGGATGCGGCGTAAACGCCTTATCCGCCCTACATGTGCAATCCCGTAGGCTGGATAAGACGCGGCAAGCGTCGCATCCGGCAATTGCACCGCGCCCCTg > 1:516203/1‑143 (MQ=255) agcGCCTTTCCTCCCTGTTAGTTGTTATTTATTGGCGGATGCGGCGTAAACGCCTTATCCGCCCTACATGTGCAATCCCGTAGGCTGGATAAGACGCGGCAAGCGTCGCATCCGGCAATTGCACCGCGCCACTGGCGGATgcg < 1:444604/141‑1 (MQ=255) cTTTACTCCCTGTTAGTTGTTATTTATTGGCGGATGCGGCGTAAACGCCTTATCCGCCCTACATGTGCAATCCCGTAGGCTGGATAAGACGCGGCAAGCGTCGCATCCGGCAATTGCACCGCGCCACTGGAGGGTGCGGCGTg > 2:35224/1‑143 (MQ=255) ttACTCCCTGTTAGTTGTTATTTATTGGCGGATGCGGCGTAAACGCCTTATCCGCCCTACATGTGCAATCCCGTAGGCTGGATAAGACGCGGCAAGCGTCGCATCCGGCAATTGCACCGCGCCACTGGCGGATGCGGCGTGaa > 2:230706/1‑143 (MQ=255) tCCCTGTTAGTTGTTATTTATTGGCGGATGCGGCGTAAACGCCTTATCCGCCCTACATGTGCAATCCCGTAGGCTGGATAAGACGCGGCAAGCGTCGCATCCGGCAATTGCACCGCGCCACTGGCGGATGCGGCGTGAACGcc < 2:615034/143‑1 (MQ=255) gTTAGTTGTTATTTATTGGCGGATGCGGCGTAAACGCCTTATCCGCCCTACATGTGCAATCCCGTAGGCTGGATAAGACGCGGCAAGCGTCGCATCCGGCAATTGCACCGCGCCACTGGCGGATGCGGCGTGAACGCCTTATc < 1:197128/143‑1 (MQ=255) aGTGGTTATTTATTGGCGGATGCGGCGTAAACGCCTCACGCGCCCTACATGTGCAATCCCGTAGGCTGGATAAGACGCGGCAAGCGTCGCATCCGGCAATTGCACCGCGCCACTGGCGGATGCGGCGTGAACGCCTTATCCGc < 1:610117/143‑1 (MQ=255) ttatttatTGGCGGATGCGGCGTAAACGCCTTATCCGCCCTACATGTGCAATCCCGTAGGCTGGATAAGACGCGGCAAGCGTCGCATCCGGCAATTGCACCGCGCCACTGGCGGATGCGGCGGGAACGCCTTATCCGCCCTAc > 1:594534/1‑143 (MQ=255) tcagtGGCTGATGCTGTGTAATCGCCTTGTCCGCCCGACATGTGCAATCCCGTAGGCTGGATAAGACGCGGCAAGCGTCGCATCCGGCAATTGCACCGCGCCACTGGCGGATGCGGCGTGAACGCCTTATCCGCCCTACAtgt < 2:549875/139‑1 (MQ=17) tagggCGGATAAGGCGTTTACGCCGCATCCGCCCTACATGTGCAATCCCGTAGGCTGGATAAGACGCGGCAAGCGTCGCATCCGGCAATTGCACCGCGCCACTGGCGGCTCCGGCGCGAACGCCTCCTCCGCCCCCCCTGTGc > 2:246532/4‑143 (MQ=17) attGGCGGATGCGGCGTAAACGCCTTATCCGCCCTACATGTGCAATCCCGTAGGCTGGATAAGACGCGGCAAGCGTCGCATc > 2:640042/1‑82 (MQ=255) attGGCGGATGCGGCGTAAACGCCTTATCCGCCCTACATGTGCAATCCCGTAGGCTGGATAAGACGCGGCAAGCGTCGCATCCGGCAATTGCACCGCGCCACTGGCGGATGCGGCGTGAACGCCTTATCCGCCCTACATGTGc < 1:304109/143‑1 (MQ=255) gggCGGATAAGGCGTTTACGCCGCATCCGCCCTACATGTGCAATCCCGTAGGCTGGATAAGACGCGGCAAGCGTCGCATCCGGCAATTGCACCGCGCCACTGGCGGATGCGGCGGGAACGCCGTATCCGCGCCACAGGTGCaa > 1:635569/2‑143 (MQ=17) gCGGATGCGGCGTATACGCCTTACCCGCCCTACATGTGCAATCCCGTAGGTTGGGTGCGGCTCCCCAAGCGTCGCATCCGGCAATTGCACCGCGCCACTGGCGGATGCGGCGTGAACGCCTTATCCGCCCTACATGTGCAATc < 1:147330/143‑1 (MQ=17) ggcggcgTGAACGCCTTATCAGCCCTACATGTGCAATCCCGTAGGCTGGATAAGACGCGGCAAGCGTCGCATCCGGCAATTGCACCGCGCCACTGGCGGATGCGGCGTGAACGCCTTATCCGCCCTACATGTGCAATCCCGTa < 1:496387/142‑1 (MQ=255) cggcgTAAACGCCTTATCCGCCCTACATGTGCACTCCCGTAGGCTGGATAAGACGCGGCAAGCGTCGCATCCGGCAATTGCACCGCGCCACTGGCGGATGCGGCGTGAACGCCTTATCCGCCCTACATGTGCAATCCCGTAgg < 1:519982/143‑1 (MQ=255) gcgTAAACGCCTTATCCGCCCTACATGTGCAATCCCGTAGGCTGGATAAGACGCGGCAAGCGTCGCATCCGGCAATTGCACCGCGCCACTGGCGGATGCGGCGCGAAGGCCTTATGCGCCCTCCAGGTGCACTCCCGCAGGca > 2:474824/1‑142 (MQ=18) aaCGCGTGACCCGCCCTACATGTGCAACCCCGTAGGCTGGATAAGACGCGGCAAGCGTCGCATCCGGCAATTGCACCGCGCCCCTGGCGGATGCGGCGTGAACGCCTTATCCGCCCTACATGTGCAATCCCGTAGGCTGGATa < 1:6518/143‑1 (MQ=18) cGCCTTATCCGCCCTACATGTGCAATCCCGTAGGCTGGATAAGACGCGGAAAGCGTCGCATCCGGGAATTGCACCGCGCCACTGGCGGATGCGGCGTGAACGGCTTATCCGCCCTACATTGGCAATCCCGTAGGCTGGAGAAg > 1:59552/1‑143 (MQ=18) cTTATCCGCCCTACATGTGCAATCCCGTAGGCTGGATAAGACGCGGCAAGCGTCGCATCCGGCAATTGCACCGCGCCACTGGCGGATGCGGCGTGAACGCCTTATCCGCCCTACATGTGCAATCCCGTAGGCTGGATAAGATg > 1:118611/1‑143 (MQ=255) tATCCGCCCTACATGTGCAATCCCGTAGGCTGGATAAGACGCGGCAAGCGTCGCATCCGGCAATTGCACCGCGCCACTGGCGGATGCGGCGTGAACGCCTTATCCGCCCTACATGTGCAATCCCGTAGGCTGGATAAGATGCg < 1:373215/143‑1 (MQ=255) | AACCGCCGAACTGCGACAGGATCCTGTCTACGGCAGCTATCAGCATAAAGAACATCATGATGTACATGATGATTTCGTTAATTCCCATCGCCTTTACTCCCTGTTAGTTGTTATTTATTGGCGGATGCGGCGTAAACGCCTTATCCGCCCTACATGTGCAATCCCGTAGGCTGGATAAGACGCGGCAAGCGTCGCATCCGGCAATTGCACCGCGCCACTGGCGGATGCGGCGTGAACGCCTTATCCGCCCTACATGTGCAATCCCGTAGGCTGGATAAGATGCG > NC_000913/2568020‑2568303 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |