| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_000913 | 475,971 | C→G | 14.4% | intergenic (+20/+11) | ybaY → / ← atl | PF09619 family lipoprotein YbaY/DNA base‑flipping protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 475,971 | 0 | C | G | 14.4% | 64.9 / 7.2 | 28 | intergenic (+20/+11) | ybaY/atl | PF09619 family lipoprotein YbaY/DNA base‑flipping protein |
| Reads supporting (aligned to +/- strand): ref base C (11/13); new base G (2/2); total (13/15) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 2.37e-01 | |||||||||||
GTGCCGGTACAGCAAACCGCCGTGCCGGTTCAGGCCAGCGGTGGCGCAACGACTACCGTACCTTCGACTTCACCAACTCAGGTGAATCCGTCTTCGGCAGTTCCAGCTCCTACGCAATATTAAGCGACGTTAACCCTCTCCGCCCGGAGAGGGTTAGTAGTTCCAGCGATAACGCTGCAAGTCGATTTGCCCGCTTCCCGATACCATCACACCTTCTGCCAGTAATGCCTGTCGCTGACGCTGTAAATCCGGTCCGGTTAGCGAAATTGTGCCGTGGCGATTAAC > NC_000913/475829‑476113 | gTGCCGGTACAGCAAACCGCCGTGCCGGTTCAGGCCAGCGGTGGCGCAACGACTACCGTACCTTCGACTTCACCAACTCAGGTGAATCCGTCTTCGGCAGGTCCAGCTCCTACGCAGTATTGAGCGCCGTTAACCCCCTCCGc > 1:161040/1‑143 (MQ=255) ggTACAGCAAACCGCCGTGCCGGTTCAGGCCAGCGGTGGCGCAACGACTACCGTACCTTCGACTTCACCAACTCAGGTGAATCCGTCTTCGGCAGTTCCAGCTCCGACGCAATATTAAGCGACGTTAACCCTCTCCGCCCggc > 1:584395/1‑142 (MQ=255) cAGCAAACCGCCGTGCCGGTTCAGGCCAGCGGTGGCGCAACGACTACCGTACCTTCGACTTCACCAACTCAGGTGAATCCGTCTTCGGCAGTTCCAGCTCCTACGCAATATTAAGCGACGTTAACCCTCTCCGCCCGGAGAgg < 1:453604/143‑1 (MQ=255) cAGCAAACCGCCGTGCCGGTTCAGGCCAGCGGTGGCGCAACGACTACCGTACCTTCGACTTCACCAACTCAGGTGAATCCGTCTTCGGCAGTTCCAGCTCCTACGCAATATTAAGCGACGTTAACCCTCTCCGCCCGGAGAgg < 1:509201/143‑1 (MQ=255) aGCAAACCGCCGTGCCGGTTCAGGCCAGCGGTGGCGCAACGACTACCGTACCTTCGACTTCACCAACTCAGGTGAATCCGTCTTCGGCAGTTCCAGCTCCTACGCAATATTAAGCGACGTTAACCCTCTCCGGGCGGAGAggg < 1:455651/143‑1 (MQ=255) cAAACCGCCGTGCCGGTTCAGGCCAGCGGTGGCGCAACGACTACCGTACCTTCGACTTCACCAACTCAGGTGAATCCGTCTTCGGCAGTTCCAGCTCCTACGCAATATTAAGCGACGTTAACCCTCTCCGCCCGGAGAGGGtt < 2:329021/143‑1 (MQ=255) cAAACCGCCGTGCCCGTTCAGGCCAGCGGTGGCGCAACGACTACCGTACCTTCGACTTCACCAACTCAGGTGAATCCGTCTTCGGCAGTTCCAGCTCCTACGCAATATTAAGCGACGTTAACCCTCTCCGGGCGGAGAGGGtt < 1:339797/143‑1 (MQ=255) atccgccgTGCCGGTTCAGGCCAGCGGTGGCGCAGCGACTACCGTACCTTCGACTTCACCAACTCATGTGCATCCGTCTTCGGCAGTTCCAGCTCCTACGCAATATTAAGCGACGTTAACCCTCTCCGCCCGGAGAGGGTtag < 2:436307/141‑1 (MQ=255) gggCCGGTTCAGGCCAGCGGTGGCGCAACGACTACCGTACCTTCGACTTCACCAACTCAGGTGAATCCGTCTTCGGCAGTTCCAGCTCCTACGCAATATTAAGCGACGTTAACCCTCTCCGCCCGGAGAGGGTTAGTAGTTcc < 2:372708/141‑1 (MQ=255) ccGGTTCAGGCCAGCGGTGGCGCAACGACTACCGTACCTTCGACTTCACCAACTCAGGTGAATCCGTCTTCGGCAGTTCCAGCTCCTACGCAATATTAAGCGACGTTAACCCTCTCCGCCCGGAGAGGGTTAGTAGTTCCAGc < 1:541214/143‑1 (MQ=255) ccAGCGGTGGCGCAACGACTACCGTACCTTCGACTTCACCAACTCAGGTGAATCCGTCTTCGGCAGTTCCAGCCCCTACGCAATATTAAGCGACGTTAACCCTCTCAGCCCGGAGAGGGCTAGTGGTCGCAGCGATGAGGCTg > 2:374515/1‑143 (MQ=255) gcAACGACTACCGTACCTTCGACTTCACGAACTCAGGTGAATCCGTCTTCGGGAGTTCCAGCGCCTACGCAATATTCAGCGACGTTATCCCTCTCCGCCGGGAGAGGGTTAGTAGTTCCAGCGATAACGCTGCAAGTCGAttt < 1:264059/143‑1 (MQ=255) cAACGACTACCGTACCTTCGACTTCACCAACTCAGGTGAATCCGTCTTCGGCAGTTCCAGCTCCTACGCAATATTAAGCGACGTTAACCCTCTCCGCCCGGAGAGGGTTAGTAGTTCCAGCGATAACGCTGCAAGTCGATTTg < 1:455852/143‑1 (MQ=255) cgtggcTTCGGCTTCTCCACCTCAGGTGAATCCGTCTTCGGCAGTTCCGGCTCCTACGCAATATTAAGCGACGTTAACCCTCTCCGCCCGGAGAGGGTTAGTAGTTCCAGCGATAACGCTGTAAGTTGATTTGCCCGCTTccc < 2:420154/138‑1 (MQ=255) ttCGACTTCACCAACTCAGGTGAATCCGTCTTCGGCAGTTCCAGCTCCTTCGCAATATTACGCGACGTTAACCATCTCCGCCCGGAGAGGGTTAGTAGTTCCAGCGATAACGCTGCAAGTCGATTTGCCCGCTTCCCGATAcc > 1:125396/1‑143 (MQ=255) ttCACCAACTCAGGTGAATCCGTCTTCGGCAGTTCCAGCTCCTACGCAATATTAAGCGACGTTAACCCTCTCCGCCCGGAGAGGGTTAGTAGTTCCAGCGATAACGCTGCAAGTCGATTTGCCCGCTTCCCGATCCCATaaca > 2:14632/1‑143 (MQ=255) ttCACCAACTCAGGTGAATCCGTCTTCGGCAGTTCCAGCTCCTACGCAATATTAAGCGACGTTAACCCTCTCCGCCCGGAGAGGGTTAGTAGTTCCAGCGATAACGCTCCACGTCGATCTTCGCGCTTCCCGCGACCATcaca > 2:280299/1‑143 (MQ=255) acctctcgTGAATTCGTCTTCGGCAGTTCCAGCTCCTACGCAATATTAAGCGACGTTAACCCTCTCCGCCCGGAGAGGGTTAGTAGTTCCAGCTATAACGCTGCAAGTCGATTTGCCCGCTTCCCGATACCATCACACCTTCt < 2:367734/136‑1 (MQ=255) aCTCAGGTCATCCCGTCTTCGGCAGTTCCAGCTCCCACGCAATATTAAGCGACTTTAACCCTCTCCGCCCGGAGAGGGTTAGTAGTTCCAGCGATAACGCTGCAAGTCGATTTGCCCGCTTCCCGATACCATCACACCTTCTg < 2:18158/143‑1 (MQ=255) cGGCAGTTCCAGCTCCTACGCAATATTAAGCGACGTTAACCCTCTCCGCCCGGAGAGGGTTAGTAGTTCCAGCGATAACGCTGCAAGTCGCTTTGCCCGCTTCCCGATACCATCACACCTTCTGCCAGTAATGCCTGTCGcgg > 1:357724/1‑141 (MQ=255) ttcTACGCAATATTAAGCGACGTTAACCCTCTCCGCCCGGAGAGGGATAGTAGTTCCAGCGATAACGCTGCAAGTCGATTTGCCCGCTTCCCGATACCATCACACCTTCTGCCAGTAATGCCTGTCGCTGACGCTGTAAAtcc > 1:443385/3‑143 (MQ=255) aCGCAATATTAAGCGACGTTAACCCTCTCCGCCCGGAGGGGGTTAGTAGTTCCAGCGATAACGCTGCAAGTCGATTTGCCCGCTTCCCGATACCATCACACCTTCTGCCAGTAATGCCTGTCGCTGACGCTGTAAAtccggtc > 1:14495/1‑143 (MQ=255) gACGTTAACCCTCTCCGCCCGGAGAGGGTTAGTAGTTCCAGCGATAACGCTGCAAGTCGATTTGCCCGCTTCCCGATACCATCACACCTTCTGCCAGTAATGCCTGTCGCTGACGCTGTAAATCCGGTCCGGTTAGCGAAAtt > 1:533684/1‑143 (MQ=255) gACGTTAACCCTCTCCGCCCGGAGAGGGTTAGTAGTTCCAGCGATAACGCTGCAAGTCGATTTGCCCGCTTCCCGATACCATCACACCTTCTGCCAGTAATGCCTGTCGCTGACGCTGTAAATCCGGTCCGGTTAGCGAAAtt < 1:498869/143‑1 (MQ=255) tgTTAACCCTCGCCGCCCGGAGAGGGTTAGTAGTTCCAGCGATAACGCTGCAAGTCGATTTGCCCGCTTCCCGATACCATCACACCTTCTGCCAGTAATGCCTGTCGCTGACGCTGTAAATCCGGTCCGGTTAGCGAAATtgt < 2:543719/142‑1 (MQ=255) tactAACCCTCTCCGGGCGGAGAGGGTTAGTAGTTCCAGCGATAACGCTGCAAGTCGATTTGCCCGCTTCCCGATACCATCACACCTTCTGCCAGTAATGCCTGTCGCTGACGCTGTAAATCCGGTCCGGTTAGCGAAATtgt > 2:36904/4‑143 (MQ=255) aaCCCTCTCCGGGCGGAGAGGGTTAGTAGTTCCAGCGATAACGCTGCAAGTCGATTTGCCCGCTTCCCGATACCATCACACCTTCTGCCAGTAATGCCTGTCGCTGACGCTGTAAATCCGGTCCGGTTAGCGAAATTGTGCCg > 2:148533/1‑143 (MQ=255) cccTCTCCGCCCGGAGAGGGTTAGTAGTTCCAGCGGTAACGCTGCAAGTCGATTTGCCCGCTTCCCGATACCATCACACCTTCTGCCAGTAATGCCTGTCGCTGACGCTGTACATCCGCTCCGGCTAGCGACATTGTGCCgcg > 2:43074/1‑141 (MQ=255) cGCCCGGAGAGGGTTAGTAGTTCCAGCGATAACGCTGCAAGTCGATTTGCCCGCTTCCCGATACCCTCCCACCGTCTGCCAGTAATGCCTGTCGCTGACGCTGTAAATCCGGTCCGGTTAGCGAAATTGTGCCGTGGCGATTa > 1:405180/1‑143 (MQ=255) cccGGAGAGGGTTAGTAGTTCCAGCGATAACGCTGCAAGTCGATTTGCCCGCTTCCCGATACCATCACACCTTCTGCCAGTCACGCCTGTCGCTGACGCTGTAAATCCGGTCCGGCTAGCGAAATTGGGCCGTGGCGATTAac > 1:642808/1‑143 (MQ=255) cccGGAGAGGGTGAGTAGTTCCAGCGATAACGCGGCAAGTCGATTTGCCCGCTTCCCGATACCATCACACCTTCTGCCAGTAATGCCTGTCGCTGACGCTGTAAATCCGGTCCGGTTAGCGAAATTGTGCCGTGGCGATTAac < 1:14632/143‑1 (MQ=255) | GTGCCGGTACAGCAAACCGCCGTGCCGGTTCAGGCCAGCGGTGGCGCAACGACTACCGTACCTTCGACTTCACCAACTCAGGTGAATCCGTCTTCGGCAGTTCCAGCTCCTACGCAATATTAAGCGACGTTAACCCTCTCCGCCCGGAGAGGGTTAGTAGTTCCAGCGATAACGCTGCAAGTCGATTTGCCCGCTTCCCGATACCATCACACCTTCTGCCAGTAATGCCTGTCGCTGACGCTGTAAATCCGGTCCGGTTAGCGAAATTGTGCCGTGGCGATTAAC > NC_000913/475829‑476113 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |