| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_000913 | 2,761,623 | C→A | 9.4% | V640L (GTA→TTA) | abpB ← | CP4‑57 prophage; putative helicase YfjK |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 2,761,623 | 0 | C | A | 9.4% | 140.5 / 5.0 | 58 | V640L (GTA→TTA) | abpB | CP4‑57 prophage; putative helicase YfjK |
| Reads supporting (aligned to +/- strand): ref base C (32/19); new base A (2/4); total (34/24) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 2.08e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.45e-01 | |||||||||||
AGCAAGTCTACCAGTCGGTGTAACGTCTGAATGGGAATGCCAATCTCTTCTAAGGCATTGACACCTGGTGGTAGATGGAAACTTTCGAACGCCAATTTGACATGTGTGTAATCGACTTTGCTGCGTATACCACGCTTTACAGCGTGGTGTTTAACTACATCCTCCATCAAGCTGAGCACCTTAGGTAGGGTATAGCCGAAGGTATTGGTTATGAGCTTCAAATCATTGTTGAGGGCAATGGAAAGAGTACGTTTCTCCCCTTCAGAAATCCACTGTCGT > NC_000913/2761484‑2761762 | aGCAAGTCTACCAGTCGGTGTAACGTCTGAATGGGAATGCCAATCTCTTCTAAGGCATTGACACCTGGTGGTAGATGGACACTTTCGAACGCCAATTTGACATGTGTGTAATCGACTTCGCTACTTCTCCCACACTTTACAGc > 2:100895/1‑143 (MQ=255)aGCAAGTCTACCAGTCGGTGTAACGTCTGAATGGGAATGCCAATCTCTTCTAAGGCATTGACACCTGGTGGTAGATGGAAACTTTCGAACGCCAATTTGACATGTGTGTAAGCGACTTTGCTGCGTATACCACGCTTAACatc > 2:80834/1‑141 (MQ=255) aaGTCTACCAGTCGGTGTAACGTCTGAATGGGAATGCCAATCTCTTCTAAGGCATTGACACCTGGTGGTAGATGGACACTTTCGCACGCAAAATTGACATGTGTGTAATCGACTTTGCTGCGCATACCACTGCTTACAGAgtg > 2:277106/1‑143 (MQ=255) aaGTCTACCAGTCGGTGTAACGTCTGAATGGGAATGCCAATCTCTTCTAAGGCATTGACACCTGGTGGTAGATGGAAACTTTCGAACGCCAATTTGACATGTGTGTAATCGACTTTGCTGCGTATACCACGCTGTAAAGCgtg < 1:13075/143‑1 (MQ=255) aGTCTACCAGTCGGTGTAACGTCTGAATGGGAATGCCAATCTCTTCTAAGGCATTGACACCTGGTGGTAGATGGAAACTTTCGAACGCCAATTTGACATGTGTGTAATCGACTTTGCTGCGTATACCACGCTTTACAGCgtgg < 1:368750/143‑1 (MQ=255) gTCTACCAGTCGGTGTAACGTCTGAATGGGAATGCCAATCTCTTCTAAGGCATTGACACCTGGTGGTAGATGGAAACTTTCGAACGCCAATTTGACATGTGTGTAATCGACTTTGCTGCGTATACCACGCTGTAAAGCgtggt < 1:418190/143‑1 (MQ=255) cTACCAGTCGGTGTAACGTCTGAATGGGAATGCCAATCTCTTCTAAGGCATTGACACCTGGTGGTAGATGGAAACTTTCGAACGCCAATTTGACATGTGTGTAATCGACTTTGCTGCGTATACCACGCTTTACAGCGTGgtgt > 1:555960/1‑143 (MQ=255) tACCAGTCGGTGTAACGGCTGAATGGGAATGCCAATCTCTTCTAAGGCATTGACACCTGGTGGTAGAAGGCATTTTTCGAACGCCAATGTGACATGTGTGTAATCGACTTAGCTGCGTAGACCACGCTGTAAAGCgtggtata < 2:630131/143‑4 (MQ=255) cgttgctgtAGCGTGTGCATGGGAATGCCTATCTCTTCTAAGGCATCGACACCTGGTGGTAGATGGAAACTTTCGAGCGCCAATTTGACATGTGTGTAATCGACTTTGCTGCGTATACCACGCTTTACAGCGTGGTGTTTAAc < 2:329704/137‑1 (MQ=255) gTCGGTGTAACGTCTGAATGGGAATGCCAATCTCTTCTAAGGCATTGACACCTGGTGGTAGATGGACACTTTCGAACGCCAATTTGACATGTGTGTAATCGACTTTGCTGCGTATACCACGCTTTACAGCGTGGTGTTTAACt < 1:241126/143‑1 (MQ=255) tAACGTCTGAATGGGAATGCCAATCTCTTCTAAGGCATTGACACCTGGTGGTAGATGGAAACTTTCGAACGCCAATTTGACATGTGTGTAATCGACTTTGCTGCGTATACCACGCTTTACAGCGTGGTGTCTAACTAcctcct > 2:401325/1‑143 (MQ=255) aCGTCTGAATGGGAATGCCAATCTCTTCTAAGGCATTGACACCTGGTGGTAGATGGAAACTTTCGACCGCCAATTTGACATGTGTGTAATCGACTTTGCTGCGTATACCACGCTTTACAGCGTGGTGTTTAACTACAtcctcc > 2:608999/1‑143 (MQ=255) cgtcgtaaTGGGAATGCTAATCTCGTCTAAGGTATTGAATCCCTGCGGTAAATGGAAACTTTCGAACGCCAATTTGACATGTGTGTAATCGACTTTGCTGCGTATACCACGCTTTACAGCGTGGTGTTTAACTACATCCTCCa < 1:437625/137‑1 (MQ=255) gactGGGAATGCCAATCGCTTCTAAGGCATCTACACGTGGTGGTAGATGGAAACTTTCGAACGCCAATTTGACATGTGTGTAATCGACTTTGCTGCGTATACCACGCTTTACAGCGTGGTGTTTAACTACATCCTCCATCAAg < 2:175516/140‑1 (MQ=255) gAATGGGAATGCCAATCTCTTCTAAGGCATTGACACCTGGTGGTAGATGGAAACTTTCGAACGCCAATTTGACATGTGTGTAATCGACTTTGCTGCGTATACCACGCTTTACAGCGTGGTGTTTAACTACATCCTCCATcggg > 1:291353/1‑140 (MQ=255) aTGGGAATGCCAATCTCTTCTAAGGCATTGACACCTGGTGGTAGCTGGAAACTTTCGAACGCCAATTTGACATGTGTGTAATCGACTTTGCTGCGTATACCACGCTTTACAGCGTGGTGTTTAACTACATCCTCCATCAAGCt < 1:640417/143‑1 (MQ=255) aaTCTCTTCTAAGGCATTGACACCTGGTGGTAGATGGAAACTTTCGAACGCCAATTTGACATGTGTGTATTTGTCTTTGCTGCGTATACCACGCTTTACAGCGTGGTGTTTAACTACATCCTCCATCAAGCTGAGCACCTtag < 2:62825/143‑1 (MQ=255) aaTCTCTTCTAAGGCATTGACACCTGGTGGTAGATGGAAACTTTCGAACGCCAATTTGACATGTGTGTAATCGACTTTGCTGCGTATACCACGCTTTACAGCGTGGTGTTTAACTACATCCTCCATCAAGCTGAGCACCTtag < 1:444047/143‑1 (MQ=255) aaTCTCTTCTAAGGCATTGACACCTGGTGGTAGATGGAAACTTTCGAACGCCAATTTGACATGTGTGTAATCGACTTTGCTGCGTATACCACGCTTTACAGCGTGGTGTTTAACTACATCCTCCATCAAGCTGAGCAACTtag > 2:8820/1‑143 (MQ=255) tttcttctATGGCATTGACACCTGGTGGTAGATGGAAACTTTCTAACGCCAATTTGTCATGTGTGTAATCGACTTTGCTGCGTATACCACGCTTTACAGCGTGGTGTTTAACTACATCCTCCATCAAGCTGAGCACCTtaggt < 2:521736/141‑1 (MQ=255) tctcTTCTAAGGCATTGACACCTGGTGGTAGATGGAAACTTTCGAACGCCAATTTGACATGTGTGTAATCGACTTTGCTGCGTATACCACGCTTTACAGCGTGGTGTTTAACTACATCCTCCATCAAGCTGAGCACCTtaggt > 2:215088/1‑143 (MQ=255) ttctAAGGCATTGACACCTGGTGGTAGATGGAAACTTTCGAACGCCAATTTGACATGTGTGTAATCGACTTTGCTGCGTATACCACGCTTTACAGCGTGGTGTTTAACTACATCCTCCATCACGCTGATCACCTTAGGTAggg > 1:573298/1‑143 (MQ=255) ttctAAGGCATTGACACCTGGTGGTAGATGGAAACTTTCGAACGCCAATTTGACATGTGTGTAATCGACTTTGCTGCGTATACCACGCTTTACAGCGTGGTGTTTAACTACATCCTCCATCAAGCTGAGCACCTTAGGTAggg > 2:639412/1‑143 (MQ=255) tAAGGCATTGACACCTGGTGGTAGATGGAAACTTTCGAACGCCAATTTGACATGTGTGTAATCGACTTTGCTGCGTATACCACGCTTTACAGCGTGGTGTTTAACTACATCCTCCATCAAGCTGAGCACCTTAGGTAGTGtag > 2:554179/1‑142 (MQ=255) aaGGCATTGACCCCTGGTGTTAGATGGAAACGGTCGAACGCCAAGTTGACATGTGTGTAATCGACTTTGCTGCGTATACCACGCTTTACAGCGTGGTGTTTAACTACATCCTCCATCAAGCTGAGCACCTTAGGTAGGGtata < 1:131975/143‑1 (MQ=255) ggCATTGACACCCGGTGGTAGATGGAAACTTTCGAACGCCAATTTGACATGTGTGTAATCGACTTTGCTGCGTATACCACGCTTTACAGCGTGGTGTTTAACTACATCCTCCATCAAGCTGAGCACCTTAGGTAGGGTATAGc < 1:121209/143‑1 (MQ=255) gCATTGACACCTGGTGGTAGATGGAAACTTTCGAACGCCAATTTGACATGTGTGTAATCGACTTTGCTGCGTATACCACGCTTTACAGCGTGGTGTTTAAGTACATCCTCCATCAAGCTGAGCACCTTAGGTAGGGTATAGcc > 1:496188/1‑143 (MQ=255) gCATTGACACCTGGTGGTAGATGGAAACTTTCGAACGCCAATTTGACATGTGTGTAATCGACTTTGCTGCGTATACCACGCTTTACAGCGTGGTGTTTAACTACATCCTCCATCAAGCTGAGCACCTTAGGTAGGGTATAGcc > 1:201544/1‑143 (MQ=255) gCATTGACACCTGGTGGTAGATGGAAACTTTCGAACGCCAATTTGACATGTGTGTAATCGACTTTGCTGCGTATACCACGCTTTACAGCGTGGTGTTTAACTACATCCTCCATCAAGCTGAGCACCTTAGGTAGGGTATAGcc > 2:268269/1‑143 (MQ=255) gCATTGACACCTGGTGGTAGATGGAAACTTTCGAACGCCAATTTGACATGTGTGTAATCGACTTTGCTGCGTATACCACGCTTTACAGCGTGGTGTTTAACTACATCCTCCATCAAGCTGAGCACCTTAGGTAGGGTATAGcc > 1:411745/1‑143 (MQ=255) gCACTGACACGTGGTGGTAGATGGAAACTTTCGAACGCCCATTTGATATGTGTGTAATCGACTTGGCTGCGTATACCACGCTTTACAGCGTGGTGTTTAACTACATCCTCCATCAAGCTTAGCACCTTAGGTAGGGTATAGcc < 2:521844/143‑1 (MQ=255) aTTGACACCTGGTGGTAGATGGAAACTTTCGAACGCCAATTTGACATGTGTGTAATCGACTTTGCTGCGTATACCACGCTTTACAGCGTGGTGTTTAACTACATCCTCCATCAAGCTGAGCACCTTAGGTAGGGTATAGCCGa < 2:567978/143‑1 (MQ=255) tGACACCTGGTGGTAGATGGAAACTTTCGAACGCCAATTTGACATGTGTGTAATCGACTTTGCTGCGTATACCACGCTTTACAGCGTGGTGTTTAACTACATCCTCAATCAAGCTGAGCACCTTAGGTAGGGTATAGCCGAAg < 2:434574/143‑1 (MQ=255) acaaCTGGTGGTAGATGGAAACTTTCCGACGCCAATTGGACAGGTGTGTAATAAACCGATCATCGTGTAGTGCGCGCTACAGCGTGGGGTTTAAGTACATCGTCCATCAGGCTGGGTACCGTAGGTCGGGTATCGCAGAAGGt > 2:286290/1‑143 (MQ=255) acCTGGTGGTAGATGGAAACTTTCGAACGCCAATTTGACATGTGTGTAATCGACTTTGCTGCGTATACCACGCTTTACAGCGTGGTGTTTAACTACATCCTCCATCAAGCTGAGCACCTTAGGTAGGGTATAGCCGCAGGTAt > 2:362333/1‑143 (MQ=255) ccTGGTGGTAGATGGAAACTTTCGAACGCCAATTTGACATGTGTGTAATCGACTTTGCTGCGTATACCACGCTTTACAGCGTGGTGTTTATCTACGTGCTTGATAAAGCCCAGCCCCTTAGGTAGGGTATAGCCGACGGTGtt > 2:626488/1‑143 (MQ=255) ctgctgGTAGATGGAAACTTCCGAACGCCAATTTGACATGTGTGTAATCGACTTTGCTGCGTATACCACGCTTTACAGCGTGGTGTTTAACTACATCCTCCATCAGTCGCAGCACCTTAGGTAGGGTATAGCCGAAGGTATTg < 2:527780/143‑1 (MQ=255) cTGGTGGTAGATGGAAACTTTCGAACGCCAATTTGACATGTGTGTAATCGACTTTGCTGCGTATACCACGCGTTACAGCGTGGTGTTTAACTACATCCTCCATCAAGCTGAGCACCTTAGGTAGGGTATAGCCGAAGGTATTg < 2:375837/143‑1 (MQ=255) tggtggTAGATGGAAACTTTCGAACGCCAATTTGACATGTGTGTAATCGACTTTGCTGCGTATACCACGCTTTACAGCGTGGTGTTTAACTACATCCTCCATCAAGCTGAGCACCTTAGGTAGGGTATAGCCGAAGGAAttcg > 2:15868/1‑141 (MQ=255) tAGATGGAAACTTTCGAACGCCAATTTGACATGTGTGTAATCGACTTTGCTGCGTATACCACGCTTTACAGCGTGGCGTTTAACTACATCCTCCCTCAAGCTGAGCACCTTCGGTAAGGCATAGCCGAAGGTATTgataccga > 2:175081/1‑136 (MQ=255) aGATGGAAACTTTCGAACGCAAATTTGACTTGTGTGTAGTCGACTTTGCTGCTTATACCACGCTTTAGAGCGTGGTTTTTATCTACATCCTCTATCAAGCTGTGCACCTTAGGTAGGTTATAGCCGAAGGTATTGGTTATGAg < 2:247434/143‑1 (MQ=255) gAAACTTTCGAACGCCAATTTGACATGTGTGTAATCGACTTTGCTGCGTATACCACGCTTTACAGCGTGGTGTTTAACTACATCCTCCATCAAGCTGAGCACCTTAGGTAGGGTATAGCCGAAGGTATTGGTTATGAGCTTCa < 2:246428/143‑1 (MQ=255) aaaCTTTCGAACGCCAATTTGACATGTGTGTAATCGACTTTGCTGCGTATACCACGCTTTACAGCGTGGTGTTTAACTACATCCTCCATCAAGCTGAGCACCTTAGGTAGGGTATAGCCGAAGGTATAGGCGATGAGCTTCaa > 1:398704/1‑143 (MQ=255) aaCTTTCGAACGCCAATTTGACATGTGTGTAATCGACTTTGCTGCGTATACCACGCTTTACAGCGTGGTGTTTAACTACATCCTCCATCGAGCTGAGCACCTTAGGTAGGGTATAGCCGAAGGTGTTGGTTATGAGCTTCaac > 1:53912/1‑142 (MQ=255) aaCGCCAATTTGACATGTGTGTAATCGACTTTGCTGCGTATACCACGCTTTACAGCGTGGTGTTTAACTACATCCTCCATCAAGCTGAGCACCTTAGGTAGGGTATAGCCGAAGGTATTGGTTATGAGCTTCAAATCAttgtt > 1:190015/1‑143 (MQ=255) ccttttGACCTGTGTGCAATCGACTTTGCTGCGGATACCACGCTTTACAGCGTGGGGTCTACCTTCCGCCTCCCGCAAGCGGAGCACCTTCTGTAGGGTATAGCCGAAGGTATTGGTTATGAGCTTCAAATCATTGTTGAggg < 2:6660/140‑1 (MQ=255) cAATTTGACATGTGTGTAATCGACTTTGCTGCGTATACCACGCTTTACAGCGTGGTGTTTAACTACATCCTCCATCAAGCTGAGCACCTTAGGTAGGGTATAGCCGAAGGTATTGGTTATGAGCTTCAAATCATTGTTGAggg > 1:276259/1‑143 (MQ=255) tGACATGTGTGTAACCCACTTTGCTGCGTATACCACGCTTTAAAGCGTAGTGTTTAACTACAACCTTCAGCCAGCTGAGCACCTTAGGTAGGGTATAGCCGAAGGTATTGGTTATGAGCTTCAAATCATTGTTGAGGGCAATg < 1:504139/143‑1 (MQ=255) gtgtgtAATCGACTTTGCTGCGTATACCACGCTTTACAGCGTGGTGATTACCTACAGCCTCCATCAAGCTGAGCACCGTAGGTAGGGTATGGCCGAAGGTATTGGTTACGAGCTTCAAATCACTGATGAGGGCAATGGaagtc > 2:631409/1‑140 (MQ=255) tAATCGACTTTGCTGCGTATACCACGCTTTACAGCGTGGTGTTTAACTACATCCTCCATCAAGCTGAGCACCTTAGGTAGGGTATAGCCGAAGGTATTGGGTATGAGCTTCAAATCGTTGTTGGGGGCGATTGAAAGGGtgag > 2:548715/1‑140 (MQ=255) aTCGACTTTGCTGCGTATACCACGCTTTACAGCGTGGTGTTTAACTACATCCTCCATCAAGCTGAGCACCTTAGGTAGGGTATAGCCGAAGGTATTGGTTATGAGCTTCAAATCATCGTTGAGGGCAAGGGAAAGAGCACGtt > 2:448237/1‑143 (MQ=255) tCGACTTTGCTGCGTATACCACGCTTTACAGCGTGGGGTTTAACTACATCCTCCATCAAGCTGAGCACCTTAGGTAGGGTATAGCCGAAGGTATTGGTTATGAGCTTCAAATCATTGTTGAGGGCAATGGAAAGAGTAGGttt > 1:416791/1‑143 (MQ=255) tgcGTATACCACGCTTTACAGCGTGGTGTTTAACTACATCCTCCATCAAGCTGAGCACCTTAGGTAGGGTATAGCCGAAGGTATTGGTTATGCGCTTCAAATCATTGTTGAGGGCAATGGAAAGAGTACGTTTCTCCCCTTCa > 2:297648/1‑143 (MQ=255) tgcGTATACCACGCTTTAAAACGTGGTTTTTAACTACATCCTCCATCAAGCTGAGCACCTTAGGTAGGGTATAGCCGAAGGTATTGGTTATGAGCTTCAAATCATTGTTGAGGGCAATGGAAAGAGTACGTTTCTCCCCTTCa > 2:567090/1‑143 (MQ=255) ttaaacaCCACGCTGTAAAGCGTGGTGTTTAACTACATCCTCCATCAAGCTGAGCACCTTAGGTAGGGTATAGCCGAAGGTATTGGTTATGAGCGTCAAATCATTGTTGAGGGCAATGGAAAGAGTACGTTTCACCCCTGCAg > 2:265824/7‑143 (MQ=255) tataCCACGCTTTACAGCGTGGTGTTTAACTACATCCTCCATCAAGCTGAGCACCTTAGGTAGGGTATAGCCGAAGGTATTGGTTATGAGCTTCAAATCATTGTTGAGGGCAATGGAAAGAGCGCGTTTCTCACCTTCAGaaa > 2:572340/1‑143 (MQ=255) cACGCTTTACAGCGTGGTGTTTAACTACATCCTCCATCAAGCTGAGCACCTTAGGTAGGGTATAGCCGAAGGTATTGGTTATGAGCTTCAAATCATTGTTGAGGGCAATGGAAAGAGTACGTTTCTCCCCTTCCGAAATCCAc > 2:37285/1‑143 (MQ=255) ttACAGCGTGGTGTTTAACTACATTCTCCATCAAGCTGAGCACCTTAGGTAGGGTATAGCCGAAGGTATTGGTTATGAGCTTCAAATCATTGTTGAGGGCAATGGAAAGAGTACGTTTCTCCCCTTCAGAAATCCCCTGTCGt < 2:494525/143‑1 (MQ=255) | AGCAAGTCTACCAGTCGGTGTAACGTCTGAATGGGAATGCCAATCTCTTCTAAGGCATTGACACCTGGTGGTAGATGGAAACTTTCGAACGCCAATTTGACATGTGTGTAATCGACTTTGCTGCGTATACCACGCTTTACAGCGTGGTGTTTAACTACATCCTCCATCAAGCTGAGCACCTTAGGTAGGGTATAGCCGAAGGTATTGGTTATGAGCTTCAAATCATTGTTGAGGGCAATGGAAAGAGTACGTTTCTCCCCTTCAGAAATCCACTGTCGT > NC_000913/2761484‑2761762 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |