| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_000913 | 3,041,632 | T→A | 100% | V107E (GTG→GAG) | ygfZ → | folate‑binding protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 3,041,632 | 0 | T | A | 100.0% | 117.1 / ‑6.7 | 42 | V107E (GTG→GAG) | ygfZ | folate‑binding protein |
| Reads supporting (aligned to +/- strand): ref base T (0/0); major base A (23/17); minor base C (0/1); total (23/19) | |||||||||||
| Rejected as polymorphism: E-value score below prediction cutoff. | |||||||||||
| Rejected as polymorphism: Frequency below/above cutoff threshold. | |||||||||||
| Rejected as polymorphism: Variant not supported by required number of reads on each strand. | |||||||||||
AAAGGTAAAATGTGGAGCAATTTACGTCTGTTCCGCGACGGCGATGGCTTTGCATGGATTGAACGGCGCAGCGTGCGTGAACCGCAGCTGACTGAACTGAAAAAATATGCGGTATTCTCTAAAGTGACCATCGCGCCAGACGACGAGCGTGTGCTGCTTGGTGTTGCCGGTTTTCAGGCGCGCGCCGCGCTGGCAAATCTCTTTAGCGAACTGCCTTCGAAAGAAAAACAGGTAGTCAAAGAAGGCGCGACCACTTTGCTATGG > NC_000913/3041508‑3041771 | aaaGGTAAAATGTGAAGCAATTTACGTGTGTTCCGCGACGGCGATGGCTTTGCATGGATTGAACGGCGCAGCGTGCGTGAACCGCAGCTGACTGAACTGAAAAAATATGCGGTATTCTCTAACGAGACCATCGCGCCAcacga > 2:633576/1‑143 (MQ=255) aTGTGGAGCAATTTACGTCTGTTCCGCGACGGCGATGGCTTTGCATGGATTGAACGGCGCAGCGTGCGTGAACCGCAGCTGACTGAACTGAAAAAATATGCGGTATTCTCTAAAGAGACCATCGCGGCAGACGGCGAGCgtgt > 2:250553/1‑143 (MQ=255) cAATTTAGGTCTGTTCCGGGAGGGCGATGGCTTTGCATGGATTGAACGGCGCAGCGTGCGTGAACCGCAGCTGATTGAACTGAAAAAATATGCGGTATTCTCTAAAGAGACCATCGCGCCAGACGACGAGCGTGTGCTGCTTg < 2:212696/143‑1 (MQ=255) cAATTTACGTCTGTTCCGCGACGGCGATTGCTTTGCTTGGATTGAACGGCGCAGCGTGCGTGAACCGCAGCTGACTGAACTGAAAAAATATGCGGTATTCTCTAACGAGACCATCGCGCCAGACGACGAGCGTGTGCTGCTTg < 2:453211/143‑1 (MQ=255) aTTTACGTCTGTTCCGCGACGGCGATGGCTTTGCATGGATTGAACGGCGCAGCGTGCGTGAACCGCAGCTGACTGAACTGAAAAAATATGCGGTATCGTCCAAAGAGACCATCGCGCCAGACGACGAGCGTGcgctgcccgcg > 2:475190/1‑138 (MQ=255) aTTTACGTCTGTGCCGCGACGGCGATGGCTTTGCATGGATTGAACGGCGCAGCGTGCGTGAACCGCAGCTGACTGAACTGAAAAAATATGCGGTATTCTCTAAAGAGACCATCGCGCCAGACGACGAGCGTGTGCTGCTTGgt < 2:448907/143‑1 (MQ=255) cgcgACGGCGATGGCTTTGCATGGATTGAACGGCGCAGCGTGCGTGAACCGCAGCTGACTGAACTGAAAAAATATGCGGTATTCTCTAAAGAGACCATCGCGCCAGACGACGAGCGTGTGCTGCTTGGTGTTGCCGGTTTTCa < 1:249953/143‑1 (MQ=255) gCTTTGCATGGATTGAACGGCGCAGCGTGCGTGAACCGCAGCTAAATGAACGATAAAAAGATGCGGTATTCTCTAAAGAGACCATCGCGCCAGACGACGAGCGTGTGCTGCTTGGTGTTGCCGGTTTTCAGGCGcgcgccgcg < 1:469966/143‑1 (MQ=255) cTTTGCATGGATTGAACGGCGCAGCGTGCGTGAACCGCAGCTGACTGAACTGAAAAAATATGCGGTATTCTCTAAAGAGACCATCGCGCCAGACGACGAGCGTGTGCTGCTTGGTGTTGCCGGTTTTCAGGCGcgcgccgcgc > 1:215806/1‑143 (MQ=255) aTTGAACGGCGCAGCGTGCGTGAACCGCAGCTGACTGAACTGAAAAAATATGCGGTATTCTCTAAAGAGACCATCGCGCCAGACGACGAGCGTGTGCTGCTTGGTGTTGCCGGTTTTCAGGCGCGCGCCGCGCTGGCAAAtct > 1:198914/1‑143 (MQ=255) ggAACGGCGCAGCGGGCGTGAACCGCAGCTGACTGGACTGAAAAAATATGCGGTATTCTCTAAAGAGACCATCGCGCCAGACGACGAGCGTGTGCTGCTTGGTGTTGCCGGTTTTCAGGCGCGCGCCGCGCTGGCAAATctct < 2:220033/142‑1 (MQ=255) gAACGGCGCAGCGTGCGTGAACCGCAGCTGACTGAACTGAAAAAATATGCGGTATTCTCTAAAGAGACCATCGCGCCAGACGACGAGCGTGTGCTGCTTGGTGTTGCCGGTTTTCAGGCGCGCGCCGCGCTGGCGAATCTCtt > 1:386611/1‑143 (MQ=255) gAACGGCGCAGCGTGCGTGAACCGCAGCTGACTGAACTGAAAAAATATGCGGTATTCTCTAAAGAGACCATCGCGCCAGACGACGAGCGTGTGCTGCTTGGTGTTGCCGGTTTTCAGGCGCGCGCCGCGCTGGCAAATCTCtt > 1:418699/1‑143 (MQ=255) aaCGGCGCAGCGTGCGTGAACCGCAGCTGACTGAACTGAAAAAATATGCGGTATTCTCTAAAGAGACCATCGCGCCAGACGACGAGCGTGTGCTGCTTGGTGTTGCCGGTTTTCAGGCGCGCGCCGCGCTGGCAAATCTCttt < 1:195670/143‑1 (MQ=255) ggCGCAGCGTGCGTGAACCGCAGCTGACTGAACTGAAAAAATATGCGGTATTCTCTAAAGAGACCATCGCGCCAGACGACGAGCGTGTGCTGCTTGGTGTTGCCGGTTTTCAGGCGCGCGCCGCGCTGGCAAATCTCTTTAGc > 1:229730/1‑143 (MQ=255) gcAGCGTCCGTGAACCGCAGCTGGCTGAACTGAAAAAATATGCGGTATTCTCTAAAGAGACCATCGCGCCAGACGACGAGCGTGTGCTGCTTGGTGTTGCCGGTTTTCAGGCGCGCGCCGCGCTGGCAAATCTCTTTAGCGaa < 1:583825/143‑1 (MQ=255) cgtgcgtgAACCGCAGCTGACTGAACTGAAAAAATATGCGGTATTCTCTAAAGAGACCATCGCGCCAGACGACGAGCGTGTGCTGCTTGGTGTTGCCGGTTTTCAGGCGCGCGCCGCGCTGGCAAATCTCTTTAGCGAACTGc > 1:382178/1‑143 (MQ=255) cggacgtgAACCCCCGCCGACTGAACTGAAAAAAGATGGCGTATTCCCTAAAGAGACCATCGCGCCAGACGACGAGCGCGTGCTGCTTGGTGTTGCCGGTTTTCAGGCGCGCGCCGCGCTGGCAAATCTCTTTAGCGAACTGc < 1:413122/139‑1 (MQ=255) cgtgAACCGCAGCTGACTGAACTGAAAAAATATGCGGTATTCTCTAAAGAGACCATCGCGCCAGACGACGAGCGTGTGCTGCTTGGTGTTGCCGGTTTTCAGGCGCGCGCCGCGCTGGCAAATCTCTTTAGCGAACTGCCTTc < 2:137384/143‑1 (MQ=255) cgtgAACCGCAGCTGACTGAACTGAAAAAATATGCGGTATTCTCTAAAGAGACCATCGCGCCAGACGACGAACGTGTGCTGCTTGGTGTTGCCGGTTTACAGGAGCGCGGCGCGCTGGCCAAGCGCGTTGACGAACCGccacc > 2:18642/1‑140 (MQ=255) gAACCGCAGCTGACTGAACTGAAAAAATATGCGGTATTCTCTAAAGAGACCATCGCGCCAGACGACGAGCGTGTGCTGCTTGGTGTTGCCGGTTTTCAGGCGCGCGCCGCGCTGGCAAATCTCTTTAGCGAACTGCCTTCgaa > 1:494979/1‑143 (MQ=255) aaCCGCAGCTGACTGAACTGAAAAAATATGCGGTATTCTCTAAAGAGACCATCGCGCCAGACGACGAGCGTGTGCTGCTTGGTGTTGCCGGTTTTCAGGCGCGCGCCGCGCTGGCAAATCTCTTTAGCGAACTGCCTTCgaaa < 2:364432/143‑1 (MQ=255) aaCCGCAGCTGACTGAACTGAAAAAATATGCGGTATGCTCTAAAGAGACCATCGCGCCAGACGACGAGCGTGTGCTGCTTGGTGTTGCCGGTTTTCAGGCGCGCGCCGCGCTGGCAAATCTCTTTAGCGAACGGCCTTCgaaa < 1:124895/143‑1 (MQ=255) gccGCAGCTGCCTGAACTGAAAAAATATGCGGTATTCTCTAAAGAGACCATCGCGCCTGGCGCGGGGCGTGTGCTGCTTCGTGTTGCCGGTTTTCAGGCGCGCGCCGAGCTGGCAAATCTCTTTAGCGAACTGCCTTCgaaag < 2:259678/142‑1 (MQ=255) ccGCAGCAGACCGTACTGAAAAAATATGCGGTATTCTCTAAAGAGACCATCGCGCCAGACGACGAGCGTGTGCTGCTTGGTGTTGCCGGTTTTCAGGCGCGCGCCGCGCTGGCAAATGTCCTTAGCGACCTGGCTCCgaacga > 2:289967/1‑143 (MQ=255) ctgaactgaaAAAATATGCGGTATTCTCTAAAGAGACCATCGCGCCAGACGACGAGCGTGTGCTGCTTGGTGTTGCCGGTTTTCAGGCGCGCGCCGCGCTGGCAAATCTCTTTAGCGAACTGCCTTCGCAAGAAAACCAGGTa > 2:474695/1‑143 (MQ=255) cagaactgaaAAAATATGACCAACACTCTCAAGGAACAATGCGGGCATACGGCGAGCATGTGCTGCAAGATTTTGCAGATATTCAGGCGAGCGCCGCGATGGCAAATCTCTTTAGCGAACTGCATTAGAAAGAAAAACAGGTa < 2:221997/141‑1 (MQ=255) aactgaaAAAATATGCGGTATTCTCTAAAGAGACCATCGCGCCAGACGACGCGCGTGTGCTGCTTGGTGTTGCCGGTTTTCAGGCGCGCGCCGCGCTGGGCGATGTCTTTAGCGCACTGCCGCCGTAGGACAAACAgggcgtg > 2:148194/1‑138 (MQ=255) tgaaAAAGTATGCGGTATGCTCGAAGAAGACCATGGGGCCAGACGACGAGCGTGTGCTGCTTGGTGTTGCCGGTTTTCAGGCGCGCGCCGCGCTGGCAAATCTCTTTAGCGAACTGCCTTCGAAAGAAAAACAGGTAGTCaaa < 2:502834/143‑1 (MQ=255) gaaAAAATATGCGGTATTCTCTAAAGAGACCATCGCGCCAGACGACGAGCGTGTGCTGCTTGGTGTTGCCGGTTTTCAGGCGCGCGCCGCGCTGGCAAATCTCTTTAGCGAACTGCCTTCGAAAGAAAAACAGGTAGTCAaag < 1:530437/143‑1 (MQ=255) aaaaaaTATGCGGTATTCTCTAAAGAGACCATCGCGCCAGACGACGAGCGTGTGCTGCTTGGTGTTGCCGGTTTTCAGGCGCGCGCCGCGCTGGCAAATCTCTTTAGCGAACTGCCTTCGAAAGAAAAACAGGTAGTCAaaga > 1:253276/1‑143 (MQ=255) aaaaaTATGCGGTATTCTCTAAAGAGACCATCGCGCCAGACGACGAGCGTGTGCTGCTAGGTGGTGCCGGTTTTCAGGCGCGCGCCGCGCTGGCAAATCTCTCTAGCGAACTGCCTTCGAAAGAAAAACAGGTAGTCAaagaa > 2:76481/1‑143 (MQ=255) aaaaTATGCGGTATTCTCTAATGAGACCATCGCGCCAGACGACGAGCGTGTGCTGCTTGGTGTTGGCGGTTTTCAGGCGCGCGCCGCGCTGGCAAATGTCTGTAGCGAACTGCCTTCGAAAGAACAGCAGGTAGTCAaagaga > 2:206104/1‑141 (MQ=255) aaaaTAGGCGGTAGTCGAGAAAGAGACCAGCGCGCCAGACGACGAGCGTGTGCTGCGTGGTGTTGCCGGTTTTCAGGCGCGCGCCGCGCTGGCAAATCTCTTTAGCGAACTGCCTTCGAAAGAAAAACAGGTAGTCAaagaag < 2:367028/143‑1 (MQ=255) aaTATGCGGTATTCTCGAAAGAGACCATCGCGCCAGACGACGAGCGTGTGCTGCTTGGTGTTGCCGGTTTTCAGGCGCGCGCCGCGCTGGCAAATCTCTTTAGCGAGCTGCCTTCGAAAGAAAAACAGGTAGTCAAAGAAGgc < 1:418308/143‑1 (MQ=255) ttCTCTAAAGAGACCATCGCGCCAGACGACGAGCGTGTGCTGCTTGGTGTTGCCGGTTTTCAGGCGCGCGCCGCGCTGGCAAATCGCTTTAGCGAACTGCCTTCGAAAGAAAAACAGGTAGTCAAAGAAGGCGCGACCACttt > 1:327626/1‑143 (MQ=255) tctcTAAAGCGACCATCGCGCCAGACGACCAGCGTGTGCTGCATGGTGTCGCCGGTTTTCAGGCGCGCGCCGCGCTGGCAAATCTCTTTAGCGAACTGCCTTCGAAAGAAAAACAGGTAGTCAAAGAAGGCGCGACCACTTTg < 1:403211/143‑1 (MQ=255) tctAAAGAGACCATCGCGCCAGCCGACGAGCGTGTGCTGCTTGGTGTTGCCGGTTTTCAGGCGCGCGCCGCGCTGGCAAATCTCTTTAGCGAACTGCCCTCGAGAGCAAAACAGGCAGTCACAGAAGGCCCGCCCACtcggct > 2:358100/1‑138 (MQ=255) tAAAGAGACCATCGCGCCAGACGACGAGCGTGTGCTGCTTGGTGTTGCCGGTTTTCAGGCGCGCGCCGCGCTGGCAAATCTCTTTAGCGAACTGCCTTCGCAAGAAAAAAAAGTTGTCAAAGAAGGCGCGACCACTTTGCTAt > 2:281994/1‑143 (MQ=255) tAAAGAGACCATCGCGCCAGACGACGAGCGTGTGCTGCTTGGTGTTGCCGGTTTTCAGGCGCGCGCCGCGCTGGCAAATCTCTTTAGCGAACTGCCTTCGAAAGAAAAACAGGTAGTCAAAGAAGGCGCGACCACTTTGCTAt > 2:197711/1‑143 (MQ=255) aaaGAGACCATCGCGCCAGACGACGAGCGTGTGCTGCTTGGTGTTGCCGGTTTTCAGGCGCGCGCCGCGCTGGCAAATCTCTTTAGCGAACTGCCTTCGAAAGAAAAACAGGTAGTCAAAGAAGGCGCGACCACTTTGCTATg > 1:493789/1‑143 (MQ=255) aaGAGACCATCGCGCCAGACGACGAGCGTGTGCTGCTTGGTGTTGGCGGTTTTCAGGCGCGGGCCGCGCTGGGAAATCTCTTTAGCGAACTGCCTTCGAAAGAAAAAGAAGTAGTCAAAGAAGGCGCGACCACTTTgacgtgg > 2:96402/1‑137 (MQ=255) | AAAGGTAAAATGTGGAGCAATTTACGTCTGTTCCGCGACGGCGATGGCTTTGCATGGATTGAACGGCGCAGCGTGCGTGAACCGCAGCTGACTGAACTGAAAAAATATGCGGTATTCTCTAAAGTGACCATCGCGCCAGACGACGAGCGTGTGCTGCTTGGTGTTGCCGGTTTTCAGGCGCGCGCCGCGCTGGCAAATCTCTTTAGCGAACTGCCTTCGAAAGAAAAACAGGTAGTCAAAGAAGGCGCGACCACTTTGCTATGG > NC_000913/3041508‑3041771 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |