| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_000913 | 3,602,701 | T→C | 27.5% | Q16R (CAG→CGG) | ftsE ← | cell division protein FtsE |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 3,602,701 | 0 | T | C | 27.5% | 37.3 / 2.6 | 27 | Q16R (CAG→CGG) | ftsE | cell division protein FtsE |
| Reads supporting (aligned to +/- strand): ref base T (5/14); new base C (3/5); total (8/19) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 6.58e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
GCCGCTAAACCAGATTTTCCCGGCGCTGGGCCGCTCAATCCCACAGATCAGCTTCAGGAGGGTACTTTTCCCTGCGCCGGAATGACCGGTCAGAAACGCCATCTCACCCGGCTGCATATGGAACGTAACGCCCTGCAGCGCCTGTCTCCCACCGAGATAAGCCTTGCTGACATGTTCAAAGCGAATCATTGTTAATCCTCTCGGGCAAAAAGTGCCTCTATAAAGTCGTCCGCCTTAAACGGACGCAAATCCTCAATACGTTCGCCGACACCAATGTA > NC_000913/3602559‑3602836 | gCCGCTGAATCAGATTTTCCCGGCGCTGTGCCGTTCAATCCCACAGATCAGCTTCAGGAGGGTACTTTTCCCTGCGCCGGAATGACCGGTCAGAAACGCCATCTCACCCGGCTGCATATGGAACGTAACGCCCTGCAGCGCCt < 2:400539/143‑1 (MQ=255) cTAAACCAGATTTTCCCGGCGCTGGGCCGCTCAATCCCACAGATCAGCTTCAGGAGGGTACTTTTCCCTGCGCCGGAATGACCGGTCAGAAACGCCATCTCACCCGGCTGCATATGGAACGTAACGCCCTGCCGCGCCTGtct > 1:106109/1‑143 (MQ=255) ccAGATTTTCCCGGCGCTGGGCCGCTCAATCCCACAGATCAGCTTCAGGAGGGTACTTTTCCCTGCGCCGGAATGACCGGTCAGAAACGCCATCTCACCCGGCTGCATATGGAACGTAACGCCCTGCAGCGCCTGTCTCCCAc < 1:519394/143‑1 (MQ=255) cAGATTTTCCCGGCGCTGGGCCGCTCAATCCCACAGATCAGCTTCAGGAGGGTACTTTTCCCTGCGCCGGAATGCCCGGTCAGCAACGCCATCTAAGCTGGCCCCACATGGAACGTAACACCCTGCAGCGCCCGTCTcccccc > 1:639293/1‑143 (MQ=255) ccGCTCAATCCCACAGATCAGCTTCAGGAGGGTACTTTTCCCTGCGCCGGAATGACCGGTCAGAAACGCCATCTCACCCGGCTGCATATGGAACGTAACGCCCTGCAGCGCCTGTCTCCCACCGAGATAAGCCTTGCTGACAt < 1:57873/143‑1 (MQ=255) cTCAATCCCACAGATCAGCTTCAGGAGGGTACTTTTCCCTGCGCCGGAATGACCGGTCAGAAACGCCATCTCACCCGGCTGCATATGGAAGGTTACGGGGAGAAGCCCCCGTCTCCCACCGACACCACCCTTGCTGCCATgcc > 1:144072/1‑141 (MQ=255) aaccccACAGACCAGCTTCAGGAGGGTACTTTTCCCTGCGCCGGAATGACCGGTCAGAAACGCCATCTCACCCGGCTGCATATGGAACGTAACGCCCTGCAGCGCCTGTCTCCCACCGAGATAAGCCTTGCTGACATGTTCaa < 1:346849/140‑1 (MQ=255) cataTCAGCTTCAGGAGGCTACTTTTCTCTTCTCTATAATGTCCGGTCAGATACGCCATCTCCCCCGGCTGCATATGGTACGTTTTTTTTTTAATGATACGGCGACCACCGAGATAAGCCTTGCTGACATGTTCAAAGCGAAt < 2:102495/140‑1 (MQ=255) accAGCGTCAGGAGGGGACTTTGCCCGGCGCCGGAATGACCGGTCAGAACCGCCATCTCACCCGGCTGCATATGGAACGTAACGCCCTGCAGCGCCTGTCTCCCACCGAGATAAGCCTTGCTGACATGTTCAAAGCGAATCAt < 1:502560/141‑1 (MQ=255) tgaGCGTCAGGAGGGTACTTTGGCCTGCGCCGGAATGACCGGTCAGAAACGCCATCTCACCCGGCTGCATATGGAACGTATCGTTCTGAAGGGCACGGCGACCACCGAGATAAGCCTTGCTGACATGTTCAAAGCGAATCAtt < 2:351516/141‑1 (MQ=255) ccAGCGTCAGGAGGGTACTTTTCCCTGCGCCGGAATGATCGGTCAGAAACGCCATCTCACCCGGCTGCATATGGAACGTTTCGTTTTGCAGGGTCCGGCGACCACCGAGATAAGCCTTGCTGACATGTTCAAAGCGAATCAtt < 2:272282/142‑1 (MQ=255) atcTTTAGGAGGGTATTTTTCCCTGCGTCGGAATGTCCTTTCTGATTCTCCATCTCACCCGGCTGCATATGGAACGTTTTTTTTTTAATGGTACGGCGACCACCGAGATAAGCCTTGCTGACATGTTCAAAGCGAATCATTGt < 2:39343/141‑1 (MQ=255) cTTCAGGAGGGTACTTTTCCCTGCGCCGGAATGACCGGTCAGAAACGCCATCTCACCCGGCTGCATATGGAACGTAACGCCCTGCAGCGCCTGTCTCCCACCGAGATAAGCCTTGCTGACATGTTCAAAGCGAATCATTGGTa > 1:61575/1‑143 (MQ=255) aCTTTTCCCGGCGCCGGAATGACCGGTCAGAAACGCCATCTCACCCGGCTGCATATGGAACGTAACGCCCTGCAGCGCCTGTCTCCCACCGAGATAAGCCTTGCTGACATGTTCAAAGCGAATCATTGTTAATCCTCTCGGGc < 1:277895/143‑1 (MQ=255) tGCGCCGGAATGACCGGTCAGAAGCGCCAGGTCACCCGGCTGCATATGGAACGTAACGCCCTGCAGCGCCTGTCTCCCACCGAGATAAGCCTTGCTGACATGTTCAAAGCGAATCATTGTTAATCCTCTCGGGCAAAAAGTGc < 1:116707/143‑1 (MQ=255) tGCGCCGGAATGACCGGTCAGAAACGCCATCTCACCCGGCTGCATATGGAACGTAACGCCCTGCAGCGCCTGTCTCCCACCGAGATAAGCCTTGCTGACATGTTCAAAGCGAATCATTGTTAATCCTCTCGGGCAAAAAGTGc > 1:493451/1‑143 (MQ=255) tGCGCCGGAATGACCGGTCAAAAACGCCATCTGACCCGGCTGCATATGGACCGTAACGCACTGCAGCGCCTGTCTGCCACCGAGATAAGCCTTGCTGACATGGTCAAAGCGAACCATTCGTAATCATCTCGGCCaaaacgagc > 2:336513/1‑138 (MQ=255) aggACCGGTCAGGAACGCCAGCCCACCCGGCTGCATATGGAACGTAACGCCCTGCAGCGCCTGGCTCCCACCGAGATAAGTCGTGCTGACATGTTCAAAGCGAATCATTGTTAATCCTCTCGGGCAAAAAGTGCCTCTATaaa < 1:624499/141‑1 (MQ=255) aTGACCGGTCAGAAACGCCATCTCACCCGGCTGCAGATGGAACGTAACGCCCTGCAGCGCCTGTCTCCCACCGAGATAAGCCTTGCTGACATGTTCAAAGCGAATCATTGTTAATCCTCTCGGGCAAAAAGTGCCTCTATaaa < 1:328088/143‑1 (MQ=255) tGACCGGCCAGAAACGCCCTCTCACCCGGCTGCATATGGAACGTAACGCCCTGCAGCGCCTGTCTCCCACCGAGATAAGCCTTGCTGACATGTTCAAAGCGAATCATTGTTAATCCTCTCGGGCAAAAAGTGCCTCTATAAAg < 1:265712/143‑1 (MQ=255) aaCGCCATCTCACCCGGCTGCATATGGAACGTAACGCCCCGCAGCGCCCGTCTCCCACCTAGCTCAGCCTTGCCGGCCTGATCACAGCCAACCGTTCGCCGTCCTCTAGGGCGAAAAATGGCACTACAACGTCGTCAGccacc > 2:394447/1‑140 (MQ=255) gCCATCACACCCGGAAGAATATGGGACGTAACGCCCTGCAGCGCCTGTCTCCCACCGAGATAAGCCTTGCTGACATGTTCAAAGCGAATCATTGTTAATCCTCTCGGGCAAAAAGTGCCTCTATAAAGTCGTCCGCCTTAAAc < 1:497405/143‑1 (MQ=255) cgtcTGCATATTGAACATAACGCCCTGCAGCGCCTGTCTCCCACCGAGATAAGCCTTGCTGACATGTTCAAAGCGAATCATTGTTAATCCTCTCGGGCAAAAAGTGCCTCTATAAAGTCGTCCGCCTTAAACGGACGCAAATc < 1:358976/140‑1 (MQ=255) atatGGAACGTAACTCTCTTCAGCGCCCGGCTCCCACCGAGATAAGCCTTGCTGACATGTTCAAAGCGAATCATTGTTAATCCTCTCGGGCAAAAAGTGCCTCTATAAAGTCGTCCGCCTTAAACGGACGCAAATCCTCAATa < 2:149300/143‑1 (MQ=255) cGCCCCGCAGCGCCTGTCTCCCCCCGAGATAAGCCTTGCTGACATGTTCAAAGCGAATCATTGTTAATCCTCTCGGGCAAAAAGTGCCTCTATAAAGTCGTCCGCCTTAAACGGACGCAAATCCTCAATACGTTCGCCGacac < 1:124132/143‑1 (MQ=255) cTGCAGCGCCTGTCTCCCACCGAGATAAGCCTTGCTGACATGTTCAAAGCGAATCATTGTTAATCCTCTCGGGCAAAAAGTGCCTCTATAAAGTCGTCCGCCTTAAACGGACGCAAATCCTCAATACGTTCGCCGACACCAAt < 1:240698/143‑1 (MQ=255) tGCAGCGCCTGTCTCCCACCGAGATAAGCCTTGCTGACATGTTCAAAGCGAATCATTGTTAATCCTCTCGGGCCAAAAGTGCCTCTATAAAGTTGTCCGCCGAAAACCAACGCAGCTCTTTGTCACGATCGCCGACACCAATg > 1:293717/1‑143 (MQ=255) cAGCGCCTGTCTCCCACCGAGATAAGCCTTGCTGACATGTTCAAAGCGAATCATTGTTAATCCTCTCGGGCAAAAAGTGCCTCTATAAAGTCGTCCGCCTTAAACGGACGCAAATCCTCAATACGTTCGCCGACACCAATGTa < 1:374662/143‑1 (MQ=255) | GCCGCTAAACCAGATTTTCCCGGCGCTGGGCCGCTCAATCCCACAGATCAGCTTCAGGAGGGTACTTTTCCCTGCGCCGGAATGACCGGTCAGAAACGCCATCTCACCCGGCTGCATATGGAACGTAACGCCCTGCAGCGCCTGTCTCCCACCGAGATAAGCCTTGCTGACATGTTCAAAGCGAATCATTGTTAATCCTCTCGGGCAAAAAGTGCCTCTATAAAGTCGTCCGCCTTAAACGGACGCAAATCCTCAATACGTTCGCCGACACCAATGTA > NC_000913/3602559‑3602836 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |