Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,886,757 T→C 9.0% intergenic (+35/‑71) yidC → / → mnmE membrane protein insertase YidC/5‑carboxymethylaminomethyluridine‑tRNA synthase GTPase subunit

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,886,7570TC 9.0% 126.4 / 3.2 50intergenic (+35/‑71)yidC/mnmEmembrane protein insertase YidC/5‑carboxymethylaminomethyluridine‑tRNA synthase GTPase subunit
Reads supporting (aligned to +/- strand):  ref base T (25/20);  new base C (2/3);  total (27/23)
Fisher's exact test for biased strand distribution p-value = 6.51e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.27e-01

CTGTACTATATCGTCAGCAACCTGGTAACCATTATTCAGCAGCAGCTGATTTACCGTGGTCTGGAAAAACGTGGCCTGCATAGCCGCGAGAAGAAAAAATCCTGATTCGGTGAGTTTTCGCTAAAATAAGGGCGGTCAGTTGACCGCCTTTTTTCTTTTCGTAGGGCGGATAAGCACCGCGCATCCGCCACACAAAGCAACAGGAACATCATGAGCGATAATGACACTATCGTAGCCCAGGCCACGCCTCCGGGACGTGGCGGCGTTGGCATCCTGC  >  NC_000913/3886618‑3886894
                                                                                                                                           |                                                                                                                                         
cTGTACTATATCGTCAGCAACCTGGTAACCATTATTCAGCAGCAGCTGATTTACCGTGGTCTGGAAAAACGTGGCCTGCATAGCCGCGAGAAGAAAAAATCCTGATTCGGTGAGTTTTCGCTAAAATAAGGGCGGTCAGTTGa                                                                                                                                        <  1:348607/143‑1 (MQ=255)
 tGTACTATATCGTGAGCAACCTGGTAACCATTATTCAGCAGCAGCTGATTTACCGTGGTCTGGAAAAACGTGGCCTGCATAGCCGCGAGAAGAAAAAATCCTGATTCGGTGAGTTTTCGCTAAAATAAGGGCGGTCAGTTGAc                                                                                                                                       <  2:276234/143‑1 (MQ=255)
  gTACTCTCTCGTCAGCAACCTCATAATCATTATTCAGCAGCAGCTGATTGACCGTGGTCTGGACAAACGTGGCCTGCATAGCCGCGAGAAGAAAAAATCCTGATTCGGTGAGTTTTCGCTAAAATAAGGGCGGTCAGTTGAcc                                                                                                                                      <  2:3921/143‑1 (MQ=255)
  gTACTATATCGTCAGCAACCTGGTAACCATTATTCAGCAGCAGCTGATTTACCGTGGTCTGGAAAAACGTGGCCTGCATAGCCGCGAGAAGAAAAAATCCTGATTCGGTGAGTTTTCGCTAAAATAAGGGCGGTCAGTTGAcc                                                                                                                                      >  2:255684/1‑143 (MQ=255)
   tACTATATCGTCAGCAACCTGGTAACCATTATTCAGCAGCAGCTGATTTACCGTGGTCTGGAAAAACGTGGCCTGCATAGCCGCGAGAAGAAACAATCCTGATTCGGTGAGTTTTCGCTAACATAAGGGCGGGCAGTTGAACg                                                                                                                                     >  2:169689/1‑143 (MQ=255)
      tataTCGTCAGCAACCTGGTAACCATTATTCAGCAGCAGCTGATTTACCGTGGTCTGGAAAAACGTGGCCTGCATAGCCGCGAGAAGAAAAAATCCTGATTCGGTGAGTTTTCGCTAAAATAAGGGCGGTCAGTTGACCGccg                                                                                                                                  >  1:335469/1‑142 (MQ=255)
      tataTCGTCAGCAACCTGGTAACCATTATTCAGCAGCAGCTGATTTACCGTGGTCTGGAAAAACGTGGCCTGCATAGCCGCGAGAAGAAAAAATCCTGATTCGGTGAGGTTTCGCTAAAATAAGGGCGGTCAGTTGACCGCCt                                                                                                                                  >  1:27675/1‑143 (MQ=255)
      tataTCGTCAGCAACCTGGTAACCATGATTCAGCAGCAGCTGATTTACCGTGGTCTGGAAAAACGTGGCCTGCATAGCCGCGAGAAGAAAAAATCCTGATTCGGTGAGTTTTCGCTAAAATAAGGGCGGTCAACTGACCGccc                                                                                                                                  <  1:47894/143‑2 (MQ=255)
       atatCGGCAGCAACCTGGTAACCATTATTCAGCAGCAGCTGATTTACCGTGGTCTGGAAAAACGTGGCCTGCATAGCCGCGAGAAGAAGAAATCCTGATTCGGTGAGTTTTCGCTAAAATAAGGGCGGTCAGTTGACCGCCtt                                                                                                                                 <  2:470442/143‑1 (MQ=255)
           cGTCAGCAACCTGGTAACCATTATTCAGCAGCAGCTGATTTACCGTGGTCTGGAAAAACGTGGCCTGCATAGCCGCGAGAAGAAAAAATCCTGATTCGGTGAGTTTTCGCTAAAATAAGGGCGGTCAACTGACCGcccttatt                                                                                                                             <  2:9023/143‑7 (MQ=255)
                 cAACCTGGTAACCATTATTCAGCAGCAGCTGATTTACCGTGGTCTGGAAAAACGTGGCCTGCATAGCCGCGAGAAGAAAAAATCCTGATTCGGTGAGTTTTCGCTAAAATAAGGGCGGTCAGTTGACCGCCTTttttcttttc                                                                                                                       <  1:103532/143‑1 (MQ=255)
                      tGGTAACCATTATTCAGCAGCAGCTGATTTACCGTGGTCTGGAAAAACGTGGCCTGCATAGCCGCGAGAAGAAAAAATCCTGATTCGGTGAGTTTTCGCTAAAATAAGGGCGGTCAGTTGACCGCCTTTTTTCTTTTCGTAgg                                                                                                                  >  1:498779/1‑143 (MQ=255)
                       ggTAACCATTATTCAGCATCAGCCGAGTTCCCGTGGTCTGGAGAAACGTGGCCTGCATAGCCGCGAGATGAAACAATCCTGATTCGGTGAGTTTTCGCTAAAATAAGGGCGGTCAGTTGACCGCCTTTTTTCTTTTCGTAggg                                                                                                                 <  2:343301/143‑1 (MQ=255)
                         tAACCATTATTCCGCAGCAGCTGATTTACCGTGGTCTGGAAAAACGTGGCCTGCATAGCCGCGAGAAGAAAAAATCCTGATTCGGTGAGTTTTCGCTAAAATAAGGGCGGTCAGTTGACCGCCTTTTTTCTTTTCGTAGGGCg                                                                                                               <  1:18244/143‑1 (MQ=255)
                            ccATTATTCAGCAGCAGCTGATTTACCGTGGTCTGGAAAAACGTGGCCTGCATAGCCGCGAGAAGAAAAAATCCTGATTCGGTGAGTTTTCGCTAAAATAAGGGCGGTCAGTTGACCGCCTTTTTTCTTTTCGTAGGGCGGAt                                                                                                            >  1:83210/1‑143 (MQ=255)
                                   tCAGCAGCAGCTGATTTACCGTGGTCTGGAAAAACGTGGCCTGCATAGCCGCGAGAAGAAAAAATCCTGATTCGGTGAGTTTTCGCTAAAATAAGGGCGGTCAGTTGACCGCCTTTTTTCTTTTCGTAGGGCGGATACGCAcc                                                                                                     >  1:187757/1‑143 (MQ=255)
                                             cTGATTTACCGTGGTCTGGAAAAACGTGGCCTGCATAGCCGCGAGAAGAAAAAATCCTGATTCGGTGAGTTTTCGCTAAAATAAGGGCGGTCAGTTGACCGCCTTTTTTCTTTTCGTAGGGCGGATAAGCACCGCGCATCCGc                                                                                           <  1:255634/143‑1 (MQ=255)
                                               gATTTACCGTGGTCTGGAAAACCGTGGCCTGCATAGCCGCGCGAAGAAAAAATCCTGATTCGGTGAGTTTTCGCTAAAATAAGGGCGGTCAGTTGACCACCTTTTTTCTTTTCGTAGGGCGGATAAGCACCGCGCATCCGCca                                                                                         <  1:301165/143‑1 (MQ=255)
                                                    aCCGGGGTCTGGAAAAACGTGGCCTGCATAGCCGCGAGAAGAAAAAATCCTGATTCGGTGAGTTTTCGCTAAAATAAGGGCGGTCAGTTGACCGCCTTTTTTCTTTTCGTAGGGCGGATAAGCACCGCGCATCCGCCACACaa                                                                                    <  1:110515/143‑1 (MQ=255)
                                                     ccGTGGTCTGGAAAAACGTGGCCTGCATAGCCGCGAGAAGAAAAAATCCTGATTCGGTGAGTTTTCGCTAAAATAAGGGCGGTCAGTTGACCGCCTTTTTTCTTTTCGTAGGGCGGATAAGCACCGCGCATCCGCCACACaaa                                                                                   <  2:134139/143‑1 (MQ=255)
                                                       gTGGTCTGGAAAAACGTGGCCTGCATAGCCGCGAGAAGAAAAAATCCTGATTCGGTGAGTTTTCGCTAAAATAAGGGCGGTCAGTTGACCGCCTTTTTTCTTTTCGTAGGGCGGCTAAGCACCGCGCATCCGCCACACAAAGc                                                                                 >  2:258455/1‑143 (MQ=255)
                                                         ggTCTGGAAAAACGTGGCCTGCATAGCCGCGAGAAGAAAAAATCCTGATTCGGTGAGTTTTCGCTAAAATAAGGGCGGTCAGTTGACCGCCTTTTTTCTTTTCGTAGGGCGGATAAGCACCGCGCATCCGCCACACAAAGCaa                                                                               <  1:354252/143‑1 (MQ=255)
                                                           tCTGGAAAACCGTGGCCTGCATAGCCGCGAGAAGAAAAAATCCTGATGCGGTGAGTCTTCGCTAAAATGAGGGCGGTCAGTTGACCGCCTTTTTTCTTTTCGTAGGGCGGATAAGCACCGCGCATCCGCCACACAAAGCAACa                                                                             <  1:10545/143‑1 (MQ=255)
                                                           tCTGGAAAAACGTGGCCTGCATAGCCGCGAGAAGAAAAAATCCTGATTCGGTGAGTTTTCGCTAAAATAAGGGCGGTAAGTGGATCTCCTTTTTTCTTTTCGTAGGGCGGATAAGCACCGCGCATCCGCCACACAAAGCAACa                                                                             >  1:147534/1‑143 (MQ=255)
                                                             tGGAAAAACGTGGCCTGCATAGCCGCGAGAAGAAAAAATCCTGATTCGGTGAGTTTTCGCTAAAATAAGGGCGGTCAGTTGACCGCCTTTTTTCTTTTCGTAGGGCGGATAAGCACCGCGCATCCGCCACACCAAGCCAcgga                                                                           >  2:213592/1‑140 (MQ=255)
                                                                aaaaaCGTGGCCTGCATAGCCGCGAGAAGAAAAAATCCTGATTCGGTGAGTTTTCGCTGAAATAAGGGCGGTCAGTTGACCGCCTTTTTTCTTTTCGTAGGGCGGATAAGCACCGCGCATCCGCCACACCACGCACCAGGAAc                                                                        >  2:425150/1‑143 (MQ=255)
                                                                aaaaaCGTGGCCTGCATAGCCGCGAGAAGAAAAAATCCTGATTCGGTGAGTTTTCGCTAAAATAAGGGCGGTCAGTTGACCGCCTTTTTTCTTTTCGTAGGGCGGATAAGCACCGCGCATCCGCCACACAAAGCAACAGGAAc                                                                        >  1:423747/1‑143 (MQ=255)
                                                                            tGCATAGCCGCGAGAAGAAAAAATCCTGATTCGGTGAGTTTTCGCTAAAATAAGGGCGGTCAGTTGACCGCCTTTTTTCTTTTCGTAGGGCGGATAAGCACCGCGCATCCGCCACACAAAGCAACAGGAACATCATGAGCGAt                                                            >  1:477781/1‑143 (MQ=255)
                                                                                 aGCCGCGAGAAGAAAAAATCCTGATTCGGTGAGTTTTCGCTAAAATAAGGGCGGTCAGTTGACCGCCTTTTTTCTTTTCGTAGGGCGGATAAGCACCGCGCATCCGCCACACAAAGCAACAGGAACATCATGAGCGATAATGa                                                       >  1:300438/1‑143 (MQ=255)
                                                                                    cgcgAGAAGAAAAAATCCTGATTCGGTGAGTTTTCGCTAAAATAAGGGCGGTCAGTTGACCGCCTTTTTTCTTTTCGTAGGGCGGATAAGCACCGCGCATCCGCCACACAAAGCAACAGGAACATCATGAGCGATAATGacgc                                                    >  1:545423/1‑141 (MQ=255)
                                                                                    cgcgAGAAGAAAAAATCCTGATTCGGTGAGTTTTCGCTAAAATAAGGGCGGTCAGTTGACCGCCTTTTTTCTTTTCGTAGGGCGGATAAGCACCGCGCATCCGCCACACAAAGCAACAGGAACATCATGAGCGATAATGacac                                                    >  1:489213/1‑143 (MQ=255)
                                                                                           agaaAAAATCCTGATTCGGTGAGTTTTCGCTAAAATAAGGGCGGTCAGTTGACCGCCTTTTTTCTTTTCGTAGGGCGGATAAGCACCGCGCATCCGCCACACAAAGCAACAGGAACATCATGAGCGATAATGACACTATCGTa                                             <  1:219251/143‑1 (MQ=255)
                                                                                             aaaaaaTCCTGATTCGGTGAGTTTTCGCTAAAATAAGGGCGGTCAGTTGACCGCCTTTTTTCTTTTCGTAGGGCGGATAAGCACCGCGCATCCGCCACACAAAGCAACAGGAACATCATGAGCGATCATGACACGATCGTAGc                                           >  1:414384/1‑143 (MQ=255)
                                                                                                aaaTCCTCATTCGGCGACTCTTCGCTAAAAGAAGGGCGGTCAGTTGACCGCCTCTTTTCTTTTCGTAGGGCGGATAAGCACCGCGCATCCGCCACACAAAGCAACAGGAACATCATGAGCGATAATGACACTATCGTAGCCCa                                        <  1:10058/143‑1 (MQ=255)
                                                                                                  aTCCTGATTCGGTGAGTTTTCGCTAAAATAAGGGCGGTCAGTTGACCGCCTTTTTTCTTTTCGTAGGGCGGATAAGCACCGCGCATCCGCCACACAAAGCAACAGGAACATCATGAGCGATAATGACACTATCGTAGCCCacg                                      >  2:636186/1‑141 (MQ=255)
                                                                                                    ccTGATTCGGTGAGTTTTCGCTAAAATAAGGGCGGTCAGTTGACCGCCTTTTTTCTTTTCGTAGGGCGGATAAGCCCCCCGCACCCGCCACACAAAGCAACAGGAACATCATGAGCGATAACGACACCATCGCGGCGCAGGcc                                    >  2:313258/1‑143 (MQ=255)
                                                                                                    ccTGATTCGGTGAGTTTTCGCTAAAATAAGGGCGGTCAGTTGACCGCCTTTTTTCTTTTCGTAGGGCGGATAAGCACCGCGCATCCGCCACACAAAGCAACAGGAACATCATGAGCGATAATGACACCATCGTAGCCCAGGcc                                    >  2:504519/1‑143 (MQ=255)
                                                                                                     cTGATTCGGTGAGTTTTCGCTAAAATAAGGGCGGTCAGTTGACCGCCTTTTTTCTTTTCGTAGGGCGGATAAACACCGCGCATCCGCCACACAAAGCAACAGGAACATCATGAGCGATAATGACACTATCGTAGCCCAGGCCa                                   <  1:367949/143‑1 (MQ=255)
                                                                                                      tGATTCGGTGAGTTTTCGCTAAAATAAGGGCGGTCAGTTGACCGCCTTTTTTCTTTTCGTAGGGCGGATAAGCACCGCGCATCCGCCACACAAAGCAACAGGAACATCATGAGCGATAATGACACTATCGTAGCCCAGGCCAc                                  <  1:564916/143‑1 (MQ=255)
                                                                                                        aTTCGGTGAGTTTTCGCTAAAATAAGGGCGGTCAGTTGACCGCCTTTTTTCTTTTCGTAGGGCGGATAAGCACCGCGCATCCGCCACACAACGCAACAGGAACATCATGATCGTTAATGACACTATTGTATCCCATGCCACgt                                >  1:217344/1‑142 (MQ=255)
                                                                                                             gTGAGTTTTCGCTAAAATAAGGGCGGTCAGTTGACCGCCTTTTTTCTTTTCGTAGGGCGGCTAAGCACCGCGCATCCGCCACACCAAGCAACAGGAACATCATGAGCGATAATGACACTATCGTAGCCCACGCCACGCCTccc                           >  1:264200/1‑142 (MQ=255)
                                                                                                              ggAGTGTTCGCTATAATAAGCGCGGTAAGCTGCCCGCCTTTTTTCTTTTCGTAGGGCGGATAAGCACCGCGCATCCGCCACACAAAGCAACAGGAACATCATGAGCGATAATGACACTATCGTAGCCCAGGCCACGCCTCCgg                          <  2:38387/142‑1 (MQ=255)
                                                                                                                            aaTAAGAGCGTACTGTTGACCGCCTTCTTTCTTTTCGTAGGGCGGATAAGCACCGCGCATCCGCCACACAAAGCAACAGGAACATCATGAGCGATAATGACACTATCGTAGCCCAGGCCACGCCTCCGGGACGTGGCGGCGtt            <  2:477781/143‑1 (MQ=255)
                                                                                                                             ctAAGGGCGGTCAGTTGACCGCCTTTTTTCTTCTCGGAGGGCGGATAAGCACCGCGCATCCGCCACACAAAGCAACAGGAACATCATGAGCGATAATGACACTATCGTAGCCCAGGCCACGCCTCCGGGACGTGGCGGCGTTg           <  2:83692/142‑1 (MQ=255)
                                                                                                                              tAAGGGCGGTCAGTTGACCGCCTTTTTTCTTTTCGTAGGGCGGATAAGCACCGCGCATCCGCCACACAAAGCAACAGGAACATCATGAGCGATAATGACACTAGCGTAGCACAGACCACACCTCCGGGACGTGGCGGAGATgg          >  1:496083/1‑143 (MQ=255)
                                                                                                                                 aggCGGTCAACTGACCGCCTTTTTTCTTTTCGTAGGGCGGATAAGCACCGCGCATCCGCCACACAAAGCAACAGGAACATCATGAGCGATAATGACACTATCGTAGCCCAGGCCACGCCTCCGGGACGTCGCGGCGTTTGcgt       >  1:588268/2‑141 (MQ=255)
                                                                                                                                  ggCGGTCAGTTGACCGCCTTTTTTCTTTTCGTAGGGCGGATAAGCACCGCGCATCCGCCACACCAAGCAACAGGAACATCATGCGCGATAATGACACTATCGTAGCCCAGGCCACGCCTCCGGGACGTGGCGGCGTTGGcccc      >  2:612626/1‑140 (MQ=255)
                                                                                                                                  ggCGGTCAACTGACCGCCTTTTTTCTTTTCGTAGGGCGGATAAGCACCGCGCATCCGCCACACAAAGCAACAGGCACATCATGAGCGATAATGACACTATCGTAGCCCACGCCACACCTCCGGGACGTGGCACCGTTGGCCTc      >  2:369810/1‑143 (MQ=255)
                                                                                                                                    cGGTCAGTTGACCGCCTTGTTTCGTTTCGCAGGGCGGATAAGCACCGCGCATCCGCCACACAAAGCAACAGGAACATCATGAGCGATAATGACACTATCGTAGCCCAGGCCACGCCTCCGGGACGTGGCGGCGTTGGCATCCt    <  2:355530/143‑1 (MQ=255)
                                                                                                                                      gTCAGTTGACCGCCTTTTTTCTTTTCGTAGGGCGGATAAGCACCGCGCATCCGCCACACAAAGCAACAGGAACAGCATGAGCGATGATGACACTATCGTAACCCAGGCCACGCCTCCGGGACGTGGCGGCGTTGGCATCCTgc  >  1:626963/1‑143 (MQ=255)
                                                                                                                                           |                                                                                                                                         
CTGTACTATATCGTCAGCAACCTGGTAACCATTATTCAGCAGCAGCTGATTTACCGTGGTCTGGAAAAACGTGGCCTGCATAGCCGCGAGAAGAAAAAATCCTGATTCGGTGAGTTTTCGCTAAAATAAGGGCGGTCAGTTGACCGCCTTTTTTCTTTTCGTAGGGCGGATAAGCACCGCGCATCCGCCACACAAAGCAACAGGAACATCATGAGCGATAATGACACTATCGTAGCCCAGGCCACGCCTCCGGGACGTGGCGGCGTTGGCATCCTGC  >  NC_000913/3886618‑3886894

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑